1,415,015 results match your criteria Experimental Biology and Medicine [Journal]


Strength limits in mesoscaled 3Y-TZP ceramics for micro-surgical instruments.

J Mech Behav Biomed Mater 2018 Dec 4;91:99-108. Epub 2018 Dec 4.

Department of Materials Science and Engineering, The Pennsylvania State University, University Park, PA 16802, USA; Institut fuer Struktur- und Funktionskeramik, Montanuniversitaet Leoben, Leoben 8700, Austria. Electronic address:

Micro-surgical instruments are a new application for mesoscale ceramics formed using the lost mold-rapid infiltration forming (LM-RIF) process. Instrument strength and reliability are the foremost concerns for this sensitive application. It is hypothesized that increasing grain size can improve the damage tolerance of the parts associated with the transformation toughening in the 3Y-TZP material, while retaining high strength. Read More

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December 2018

Immunophenotypic measurable residual disease (MRD) in acute myeloid leukemia: Is multicentric MRD assessment feasible?

Leuk Res 2018 Nov 27;76:39-47. Epub 2018 Nov 27.

Department of Hematology, VU University Medical Center, Amsterdam, The Netherlands.

Flow-cytometric detection of now termed measurable residual disease (MRD) in acute myeloid leukemia (AML) has proven to have an independent prognostic impact. In a previous multicenter study we developed protocols to accurately define leukemia-associated immunophenotypes (LAIPs) at diagnosis. It has, however, not been demonstrated whether the use of the defined LAIPs in the same multicenter setting results in a high concordance between centers in MRD assessment. Read More

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November 2018

Distribution of mycotoxins produced by Penicillium spp. inoculated in apple jam and crème fraiche during chilled storage.

Int J Food Microbiol 2018 Dec 7;292:13-20. Epub 2018 Dec 7.

University of Natural Resources and Life Sciences, Vienna (BOKU), Department of Agrobiotechnology (IFA-Tulln), Center for Analytical Chemistry, Konrad-Lorenz-Str. 20, 3430 Tulln, Austria.

Estimations of consumer exposure to mycotoxins through surveillance of mycotoxins in the food trade are well described, but the exposure due to mouldy food in private homes is not known, and may result from removing visible mould on food and eating the rest. In this study, we followed the growth of Penicillium expansum on the surface of apple jam and Penicillium verrucosum on crème fraiche, as well as production and distribution of fungal metabolites throughout the sample (approx. 6 cm high divided into three equal layers), using a multianalyte method, over time (up to 28 days) and at 4, 8 and 15 °C. Read More

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December 2018
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Interaction of hormonal and social environments in understanding body image concerns in adolescent girls.

J Psychiatr Res 2018 Dec 7;109:178-184. Epub 2018 Dec 7.

Department of Psychology, Michigan State University, 316 Physics Rd #262, East Lansing, MI, 48824, USA.

During adolescence, peer approval becomes increasingly important and may be perceived as contingent upon appearance in girls. Concurrently, girls experience hormonal changes, including an increase in progesterone. Progesterone has been implicated in affiliative behavior but inconsistently associated with body image concerns. Read More

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December 2018

Simultaneous quantification of the degree of hydrolysis, protein conversion rate and mean molar weight of peptides released in the course of enzymatic proteolysis.

J Chromatogr B Analyt Technol Biomed Life Sci 2018 Dec 6;1105:1-9. Epub 2018 Dec 6.

Laboratoire Réactions et Génie des Procédés, Université de Lorraine, CNRS, LRGP, F-54000 Nancy, France; Université de Lorraine, 2 Avenue de la forêt de Haye, F-54505 Vandœuvre-lès-Nancy, France. Electronic address:

This paper describes an original analytical methodology for a simultaneous measurement of the protein conversion rate, the mean molar weight of peptide and the degree of hydrolysis in the course of proteolysis by Size-Exclusion High-Performance Liquid Chromatography. Peak area of dead volume eluents reflects the non-converted protein. The protein conversion rate is thus determined by comparing the area at a given time to the initial area. Read More

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December 2018

Cladophora glomerata methanolic extract decreases oxidative stress and improves viability and mitochondrial potential in equine adipose derived mesenchymal stem cells (ASCs).

Biomed Pharmacother 2018 Dec 12;111:6-18. Epub 2018 Dec 12.

Department of Experimental Biology, Faculty of Biology and Animal Science, Wrocław University of Environmental and Life Sciences, Norwida 27B, 50-375 Wrocław, Poland; Faculty of Veterinary Medicine, Equine Clinic - Equine Surgery, Justus-Liebig-University, 35392 Gießen, Germany. Electronic address:

Reactive oxygen species (ROS) are key mediators of several cellular damage and thus associated with equine diseases such as inflammation and metabolic syndrome. This study aimed to evaluate the protective and antioxidant activities of methanolic extract prepared from Cladophora glomerata (C. glomerata) biomass, on equine adipose derived mesenchymal stem cells (EqASCs), under experimental oxidative stress induced by HO. Read More

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December 2018

Harmonization of pipeline for preclinical multicenter plasma protein and miRNA biomarker discovery in a rat model of post-traumatic epileptogenesis.

Epilepsy Res 2018 Nov 26;149:92-101. Epub 2018 Nov 26.

A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland.

The Epilepsy Bioinformatics Study for Antiepileptogenic Therapy (EpiBioS4Rx) is an international, multicenter, multidisciplinary study aimed at preventing epileptogenesis (EpiBioS4Rx: https://epibios.loni.usc. Read More

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November 2018
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Noble metal nanoparticle-functionalized Zr-metal organic frameworks with excellent photocatalytic performance.

J Colloid Interface Sci 2018 Dec 6;538:569-577. Epub 2018 Dec 6.

College of Chemical Engineering, Jiangsu Key Lab for the Chemistry & Utilization of Agricultural and Forest Biomass, Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, Jiangsu 210037, China. Electronic address:

Noble metal nanoparticles (NPs) functionalized MOFs are attractive materials for photocatalytic reactions, and outstanding catalytic performances can be expected, especially when they have intrinsically matched crystal faces between metals and MOFs. This study discovered that the photocatalytic oxidation of aromatic alcohols to aldehydes was enhanced over Au/UiO-66-NH or Au/UiO-66 but suppressed over Pt loaded counterparts, whereas the reduction of Cr(VI) was boosted over both two catalysts. In reactions, the conversion of benzyl alcohol was 17. Read More

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December 2018

Enhanced volatile fatty acids production from waste activated sludge anaerobic fermentation by adding tofu residue.

Bioresour Technol 2018 Dec 7;274:430-438. Epub 2018 Dec 7.

College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.

In this study, an economical and eco-friendly strategy (i.e., adding tofu residue (TR) into waste activated sludge (WAS)) to enhance volatile fatty acid (VFA) production was reported. Read More

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December 2018

A decade of Nucleic Acid Programmable Protein Arrays (NAPPA) availability: News, actors, progress, prospects and access.

J Proteomics 2018 Dec 12. Epub 2018 Dec 12.

Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain; Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain. Electronic address:

Understanding the dynamic of the proteome is a critical challenge because it requires high sensitive methodologies in high-throughput formats in order to decipher its modifications and complexity. While molecular biology provides relevant information about cell physiology that may be reflected in post-translational changes, High-Throughput (HT) experimental proteomic techniques are essential to provide valuable functional information of the proteins, peptides and the interconnections between them. Hence, many methodological developments and innovations have been reported during the last decade. Read More

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December 2018

Physical exercise enhances adult cortical plasticity in a neonatal rat model of hypoxic-ischemic injury: Evidence from BOLD-fMRI and electrophysiological recordings.

Neuroimage 2018 Dec 12. Epub 2018 Dec 12.

Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, 06351, South Korea; Department of Radiology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 06351, South Korea; Center for Neuroscience Imaging Research, Institute for Basic Science (IBS), Suwon, South Korea; Animal Research and Molecular Imaging, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, 06351, South Korea; Department of Global Biomedical Engineering, Sungkyunkwan University, Suwon, Republic of Korea. Electronic address:

Neuroplasticity is considered essential for recovery from brain injury in developing brains. Recent studies indicate that it is especially effective during early postnatal development and during the critical period. The current study used functional magnetic resonance imaging (fMRI) and local field potential (LFP) electrophysiological recordings in rats that experienced neonatal hypoxic-ischemic (HI) injury during the critical period to demonstrate that physical exercise (PE) can improve cortical plasticity even when performed during adulthood, after the critical period. Read More

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December 2018

Variety matters: Diverse functions of monocyte subtypes in vascular inflammation and atherogenesis.

Vascul Pharmacol 2018 Dec 12. Epub 2018 Dec 12.

Cardiology and Angiology, Philipps-University Marburg, Marburg, Germany. Electronic address:

Monocytes are important mediators of the innate immunity by recognizing and attacking especially bacterial pathogens but also play crucial roles in various inflammatory diseases, including vascular inflammation and atherosclerosis. Maturation, differentiation and function of monocytes have been intensively explored for a long time in innumerable experimental and clinical studies. Monocytes do not represent a uniform cell type but could be further subdivided into subpopulations with distinct features and functions. Read More

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December 2018

Alpha-synuclein is highly prone to distribution in the hippocampus and midbrain in tree shrews, and its fibrils seed Lewy body-like pathology in primary neurons.

Exp Gerontol 2018 Dec 12. Epub 2018 Dec 12.

Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China; Medical Primate Research Center & Neuroscience Center, Chinese Academy of Medical Sciences, Beijing 100005, China; Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Diseases, Kunming 650118, China. Electronic address:

The Chinese tree shrew (TS) has many unique advantages that make it suitable for use as an experimental animal model for human disease including moderate body size, low cost of feeding, short reproductive cycle and lifespan, and close phylogenetic relationship to primates. Our previous studies have shown that TS treated with the mitochondrial inhibitor MPTP displayed classic Parkinsonian symptoms. Additionally, the structure of TS alpha-synuclein (α-syn) is highly homologous to that found in humans. Read More

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December 2018

Chitosan-hydroxypropyl methylcellulose tioconazole films: A promising alternative dosage form for the treatment of vaginal candidiasis.

Int J Pharm 2018 Dec 12. Epub 2018 Dec 12.

Instituto de Química Rosario (IQUIR, CONICET-UNR), Suipacha 531, Rosario S2002LRK, Argentina; Área Técnica Farmacéutica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario S2002LRK, Argentina. Electronic address:

Vaginal candidiasis is considered a frequent opportunistic mucosal infection and the second most common cause of vaginitis after bacterial vaginosis. In this work, different vaginal films based on chitosan, hydroxypropyl methylcellulose and blends of these polymers containing tioconazole, were developed and thoroughly characterized to improve the conventional therapeutics of vaginal candidiasis. Mechanical properties, swelling, adhesiveness, morphology, antifungal activity, hemocompatibility and cytotoxicity were evaluated. Read More

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December 2018

Preclinical Evaluation of Dose-Volume Effects and Lung Toxicity Occurring in- and out-of-field.

Int J Radiat Oncol Biol Phys 2018 Dec 12. Epub 2018 Dec 12.

Centre for Cancer Research and Cell Biology, Queen's University Belfast, Northern Ireland, UK.

Purpose: The aim of this study was to define the dose and dose-volume relationship of radiation induced pulmonary toxicities occurring in and out-of-field in mouse models of early inflammatory and late fibrotic response.

Materials And Methods: Early radiation induced inflammation and fibrosis were investigated in C3H/NeJ and C57BL/6J mice respectively. Animals were irradiated with 20 Gy delivered to the upper region of the right lung as a single fraction or as three consecutive fractions using the Small Animal Radiation Research Platform (SARRP, Xstrahl Inc. Read More

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December 2018

Efficacy and Safety of Non-Invasive Intravesical Instillation of Onabotulinum Toxin-A for Overactive Bladder and Interstitial Cystitis/Bladder Pain Syndrome: Systematic Review and Meta-analysis.

Urology 2018 Dec 12. Epub 2018 Dec 12.

Department of Urology, Soonchunhyang University Seoul Hospital, Soonchunhyang University Medical College, Seoul, South Korea. Electronic address:

Objective: To investigate the efficacy and safety of non-invasive intravesical instillation of OBTX-A through systematic review and meta-analysis. Recently, several studies of non-invasive intravesical instillation of Onabotulinum Toxin-A (OBTX-A) have been published. However, its efficacy is not well validated yet compared to well-known efficacy of minimally invasive intravesical injection of OBTX-A. Read More

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December 2018
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The influence of the OPRM1 (A118G) polymorphism on behavioral and neural correlates of aggression in healthy males.

Neuropharmacology 2018 Dec 12. Epub 2018 Dec 12.

Department of Psychiatry, Psychotherapy and Psychosomatics, Faculty of Medicine, RWTH Aachen University, 52074, Aachen, Germany; JARA Institute Brain Structure Function Relationship Institute for Neuroscience and Medicine (INM 10), Forschungszentrum Jülich, 52425 Jülich, Germany.

Current models of aggression suggest that in addition to personality traits and environmental factors, biological vulnerability associated with genetics substantially impacts aggressive behavior. In a functional imaging study, we investigated the influence of the single nucleotide polymorphism of the mu 1 subtype opioid receptor gene (OPRM1), implicated in sociability, on correlates of trait and state aggression to delineate the function of these influences in aggression. A key aim was further to differentiate different aspects of aggressive reactions - namely, the reaction to provocation and the decision to punish an opponent. Read More

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December 2018

Oxidative stress in the choroid plexus contributes to blood-cerebrospinal fluid barrier disruption during sepsis development.

Microvasc Res 2018 Dec 12. Epub 2018 Dec 12.

Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Graduate Program in Health Sciences, Health Sciences Unit, University of South Santa Catarina, Tubarão, SC, Brazil. Electronic address:

Background: The choroid plexus (CP), main component of blood-cerebrospinal fluid barrier (BCSFB), protects the brain from peripheral inflammation similar to the blood-brain barrier. Thus, CP is considered a critical target site of oxidative damage, which in sepsis oxidative stress is likely to be a major step in the development of brain damage. Functional alterations in CP may be associated with sepsis-induced brain injury. Read More

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December 2018
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Astaxanthin ameliorates scopolamine-induced spatial memory via reduced cortical-striato-hippocampal oxidative stress.

Brain Res 2018 Dec 12. Epub 2018 Dec 12.

Department of General and Experimental Psychology, Ludwig-Maximilians-Universität München, Germany. Electronic address:

Alzheimer's disease is characterized by progressive disruption of cholinergic neurotransmission and impaired cognitive functions. In rodents, scopolamine has been used to induce cholinergic dysfunction resulting in cognitive impairments and an increment of oxidative stress in the brain. Here we tested whether oxidative stress can be attenuated via an antioxidant (astaxanthin) to rescue scopolamine-induced spatial memory. Read More

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December 2018

Cardio-omentopexy Reduces Cardiac Fibrosis and Heart Failure after Experimental Pressure Overload.

Ann Thorac Surg 2018 Dec 12. Epub 2018 Dec 12.

Department of Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL; Division of Cardiovascular-Thoracic Surgery, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, IL. Electronic address:

Background: The pedicled greater omentum has been shown to offer benefit in ischemic heart disease for both animal models and human patients. The impact of cardio-omentopexy in a pressure overload model of left ventricular hypertrophy (LVH) is unknown.

Methods: LVH was created in rats by banding the ascending aorta after right thoracotomy (n=23). Read More

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December 2018

The DAHANCA 32 study: Electrochemotherapy for recurrent mucosal head and neck cancer.

Head Neck 2018 Dec 15. Epub 2018 Dec 15.

Department of Otorhinolaryngology, Head & Neck Surgery and Audiology, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark.

Background: Electrochemotherapy is an established treatment for cutaneous tumors. This study aimed at determining efficacy of electrochemotherapy in recurrent head and neck cancer.

Methods: Phase II clinical trial in patients with recurrent head and neck carcinomas with no curative treatment options. Read More

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December 2018

Receptor tyrosine kinase MET as potential target of multi-kinase inhibitor and radiosensitizer sorafenib in HNSCC.

Head Neck 2018 Dec 15. Epub 2018 Dec 15.

Laboratory of Radiobiology and Experimental Radiation Oncology, Hubertus Wald Tumorzentrum-University Cancer Center Hamburg, Hamburg-Eppendorf, Germany.

Background: The multi-kinase inhibitor sorafenib displays antitumoral effects in head and neck squamous cell carcinoma (HNSCC); however, the targeted kinases are unknown. Here we aimed to identify those kinases to determine the mechanism of sorafenib-mediated effects and establish candidate biomarkers for patient stratification.

Methods: The effects of sorafenib and MET inhibitors crizotinib and SU11274 were analyzed using a slide-based antibody array, Western blotting, proliferation, and survival assays. Read More

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December 2018

Photoacoustic response induced by nanoparticle-mediated photothermal bubbles beyond the thermal expansion for potential theranostics.

J Biomed Opt 2018 Dec;23(12):1-12

Xi'an Jiaotong University, School of Life Science and Technology, Key Laboratory of Biomedical Infor, China.

Photoacoustic responses induced by laser-excited photothermal bubbles (PTBs) in colloidal gold solutions are relevant to the theranostics quality in biomedical applications. Confined to the complexity of nonstationary, multiscale events, and multiphysical parameters of PTBs, systematic studies of the photoacoustic effects remain obscure. Photoacoustic effects mediated by PTB dynamics and a physical mechanism are studied based on a proof-of-principle multimodal platform integrating side-scattering imaging, time-resolved optical response, and acoustic detection. Read More

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December 2018

How Do Family Strengths-Oriented Therapeutic Conversations (FAM-SOTC) Advance Psychiatric Nursing Practice?

J Nurs Scholarsh 2018 Dec 15. Epub 2018 Dec 15.

Psychiatric Clinical Nurse Specialist, Landspitali University Hospital, Landspitali-The National University Hospital in Iceland, Child and Adolescent Psychiatric Division, Reykjavik, Iceland.

Purpose: Family nursing interventions, focusing on therapeutic conversations, have been found to benefit primary caregivers dealing with chronic and acute illnesses. Less is known, however, about the benefit of these interventions for partner caregivers. The aims of this study were to develop and test the Family Strengths-Oriented Therapeutic Conversation (FAM-SOTC) intervention for partner caregivers of young individuals with eating disorders (EDs). Read More

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December 2018

Effects of isoquinoline alkaloids from Macleaya cordata on physiological, immunological and inflammatory parameters in healthy beagles: Alkaloids in dog nutrition.

J Anim Physiol Anim Nutr (Berl) 2018 Dec 14. Epub 2018 Dec 14.

Institute of Animal Nutrition, Freie Universität Berlin, Berlin, Germany.

The aim of the study was to investigate nutritional, physiological and immunological effects of a plant-derived blend of isoquinoline alkaloids (Sangrovit® Extra) in healthy dogs. Two groups of healthy, adult beagles (N = 10) were tested in a cross-over experiment, lasting two consecutive three-week periods. The experimental group received 1. Read More

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December 2018

The effect of inulin supply to high-fat diet rich in saturated fatty acids on pork quality and profile of sarcoplasmic protein in meat exudate.

J Anim Physiol Anim Nutr (Berl) 2018 Dec 14. Epub 2018 Dec 14.

Department of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences - SGGW, Warsaw, Poland.

The aim of the study was to evaluate the influence of inulin supply to high-fat diet rich in saturated fatty acids (SFA) on pork quality and profile of sarcoplasmic protein in drip loss. At 50 days of age, twenty cross-bred pigs (gilts) were randomly allotted to four groups: the control (C) group fed a standard diet, and three experimental (D1, D2 and D3) groups fed a high-fat diet rich in SFA. Moreover, pigs from the groups D2 and D3 consumed an extra inulin supply (7% of daily feed intake) from 85 to 120 days of age (for 5 weeks) and from 50 to 120 days of age (for 10 weeks) respectively. Read More

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December 2018

Genetically engineered animal models of Parkinson's disease: from worm to rodent.

Eur J Neurosci 2018 Dec 14. Epub 2018 Dec 14.

Laboratory of Functional Chemistry, IRCCS Mondino Foundation, Pavia, 27100, Italy.

Parkinson's disease (PD) is a progressive neurological disorder characterised by aberrant accumulation of insoluble proteins, including alpha-synuclein, and a loss of dopaminergic neurons in the substantia nigra. The extended neurodegeneration leads to a drop of striatal dopamine levels, responsible for disabling motor and non-motor impairments. Although the causes of the disease remain unclear, it is well accepted among the scientific community that the disorder may also have a genetic component. Read More

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December 2018
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Non-adherence to psychotropic medication assessed by plasma levels in newly admitted psychiatric patients.

Psychiatry Clin Neurosci 2018 Dec 15. Epub 2018 Dec 15.

Medical University Innsbruck, Department of Psychiatry, Psychotherapy and Psychosomatics, Division of experimental Psychiatry and Psychotherapy, Austria.

Aims: Non-adherence or partial adherence to psychotropic medication is found in 18-70% of patients. However, many previously used methods for the assessment of adherence (e.g. Read More

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December 2018

HBcAg-induced up-regulated 4-1BB ligand on B cells contributes to B cell hyperactivation during chronic hepatitis B infection.

J Med Virol 2018 Dec 14. Epub 2018 Dec 14.

Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.

During the natural history of chronic hepatitis B infection (CHB) the function of B cells is still obscure. Several limited studies have suggested that B cells are highly active in CHB patients. In present work, we reported that the 4-1BB ligand (4-1BBL) expression on B cells was significantly higher in CHB patients than that in healthy subjects, meanwhile the CHB patients had higher serological IgG levels. Read More

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December 2018
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Sympatric diploid and tetraploid cytotypes of Centaurea stoebe s.l. do not differ in arbuscular mycorrhizal communities and mycorrhizal growth response.

Am J Bot 2018 Dec 14. Epub 2018 Dec 14.

Department of Botany, Faculty of Science, Charles University, Benátská 2, CZ-128 01, Prague, Czech Republic.

Premise Of The Study: Genome duplication is associated with multiple changes at different levels, including interactions with pollinators and herbivores. Yet little is known whether polyploidy may also shape belowground interactions.

Methods: To elucidate potential ploidy-specific interactions with arbuscular mycorrhizal fungi (AMF), we compared mycorrhizal colonization and assembly of AMF communities in roots of diploid and tetraploid Centaurea stoebe s. Read More

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December 2018

From avatars to body swapping: The use of virtual reality for assessing and treating body-size distortion in individuals with anorexia.

J Clin Psychol 2018 Dec 15. Epub 2018 Dec 15.

Department of Psychology, Università Cattolica del Sacro Cuore, Milan, Italy.

In the last 30 years, virtual reality (VR) has offered innovative solutions for assessing and treating body representation disturbances in anorexia nervosa (AN). The most recent and innovative trend is the exploitation of the so-called VR-based body swapping illusion. The aim of this case study was to report the use of this VR protocol within a multidisciplinary treatment of AN. Read More

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December 2018

Alternative transcript imbalance underlying breast cancer susceptibility in a family carrying PALB2 c.3201+5G>T.

Breast Cancer Res Treat 2018 Dec 14. Epub 2018 Dec 14.

Oncogenetics Group, Vall d'Hebron Institute of Oncology, VHIO, 08035, Barcelona, Spain.

Purpose: Disruption of splicing motifs by genetic variants can affect the correct generation of mature mRNA molecules leading to aberrant transcripts. In some cases, variants may alter the physiological transcription profile composed of several transcripts, and an accurate in vitro evaluation is crucial to establish their pathogenicity. In this study, we have characterized a novel PALB2 variant c. Read More

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December 2018

Cell-Based Model of the Generation and Maintenance of the Shape and Structure of the Multilayered Shoot Apical Meristem of Arabidopsis thaliana.

Bull Math Biol 2018 Dec 14. Epub 2018 Dec 14.

Department of Mathematics, University of California, Riverside, USA.

One of the central problems in animal and plant developmental biology is deciphering how chemical and mechanical signals interact within a tissue to produce organs of defined size, shape, and function. Cell walls in plants impose a unique constraint on cell expansion since cells are under turgor pressure and do not move relative to one another. Cell wall extensibility and constantly changing distribution of stress on the wall are mechanical properties that vary between individual cells and contribute to rates of expansion and orientation of cell division. Read More

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December 2018

Molecular mimicry, genetic homology, and gene sharing proteomic "molecular fingerprints" using an EBV (Epstein-Barr virus)-derived microarray as a potential diagnostic method in autoimmune disease.

Immunol Res 2018 Dec 15. Epub 2018 Dec 15.

Rheumatology, Boston University School of Medicine, Arthritis Center, 72 E. Concord Street, E-5, Boston, MA, 02118, USA.

EBV (Epstein-Barr Virus) and other human DNA viruses are associated with autoimmune syndromes in epidemiologic studies. In this work, immunoglobulin G response to EBV-encoded proteins which share regions with human immune response proteins from the human host including ZEBRA (BZLF-1 encoded protein), BALF-2 recombinase expressed primarily during the viral lytic replication cycle, and EBNA-1 (Epstein-Barr Virus Nuclear Antigen) expressed during the viral latency cycle respectively were characterized using a laser-printed micro-array ( PEPperprint.com ). Read More

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December 2018
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FcRγ deficiency improves survival in experimental sepsis by down-regulating TLR4 signaling pathway.

Immunol Res 2018 Dec 14. Epub 2018 Dec 14.

Department of Oncology, General Hospital of Chinese PLA, 28 Fuxing Road, Beijing, 100853, China.

Fc receptor common γ signaling chain (FcRγ), a common subunit shared by Fc receptors (FcγRI, III, IV, FcαRI, and FcεRI), is an important immune regulator both in innate and adaptive immunity. Previous studies have shown that FcRγ was a potential target of inflammatory diseases, whereas the role of FcRγ in sepsis has been poorly understood. In this study, we found that deficiency of FcRγ resulted in increased survival in lipopolysaccharide (LPS)/D-galactosamine and E. Read More

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December 2018

Sensitivity of physiological and biochemical endpoints in early ontogenetic stages of crops under diclofenac and paracetamol treatments.

Environ Sci Pollut Res Int 2018 Dec 14. Epub 2018 Dec 14.

Laboratory of Metabolomics and Isotope Analyses, Global Change Research Institute, Czech Academy of Sciences, Bělidla 986/4a, 603 00, Brno, Czech Republic.

Early stages of ontogenesis determining subsequent growth, development, and productivity of crops can be affected by wastewater and sludge contaminated with pharmaceuticals. Diclofenac (DCF) and paracetamol (PCT; both 0.0001 to 10 mg/L) did not affect seed germination and primary root length of onion, lettuce, pea, and tomato. Read More

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December 2018
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Fulminant mucormycosis after a traffic accident: a case report.

Folia Microbiol (Praha) 2018 Dec 14. Epub 2018 Dec 14.

Department of Microbiology, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, 9 M. Sklodowska-Curie Street, 85-094, Bydgoszcz, Poland.

Mucormycosis is a rare fungal infection in immunocompetent patients, whereas in immunocompromised, it may be systemic and disseminated infection associated with high mortality. Mucormycosis is one of the most rapidly progressing and fulminant forms of fungal infections; Mucor circinelloides is rarely isolated species, also from immunocompromised patients. The reported case of mucormycosis after a traffic accident indicates that it may be the result of a contamination of wound by M. Read More

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December 2018

The influence of age on the psychological profile of patients with cardiac implantable electronic devices: results from the Italian population in a multicenter study conducted by the European Heart Rhythm Association.

Aging Clin Exp Res 2018 Dec 14. Epub 2018 Dec 14.

Department of Electrophysiology, Heart Center of Leipzig, Leipzig, Germany.

Background: Cardiac implantable electronic devices (CIEDs) are widely used to treat bradyarrhythmias or improve the prognosis of patients with heart failure (HF).

Aims: To evaluate age-related (≤ 75 vs. > 75 years) attitudes, worries, psychological effects and needs in an Italian CIEDs population. Read More

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December 2018

Therapeutic potentials of the Rho kinase inhibitor Fasudil in experimental autoimmune encephalomyelitis and the related mechanisms.

Metab Brain Dis 2018 Dec 14. Epub 2018 Dec 14.

Institute of Brain Science, Shanxi Datong University, Datong, China.

Multiple sclerosis (MS), Parkinson's disease (PD), Alzheimer's disease (AD), and other neurodegenerative diseases of central nervous system (CNS) disorders are serious human health problems. Rho-kinase (ROCK) is emerging as a potentially important therapeutic target relevant to inflammatory neurodegeneration diseases. This is supported by studies showing the beneficial effects of fasudil, a ROCK inhibitor, in inflammatory neurodegeneration diseases. Read More

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December 2018

JPlogP: an improved logP predictor trained using predicted data.

J Cheminform 2018 Dec 14;10(1):61. Epub 2018 Dec 14.

Lhasa Limited, Granary Wharf House, 2 Canal Wharf, Leeds, LS11 5PS, UK.

The partition coefficient between octanol and water (logP) has been an important descriptor in QSAR predictions for many years and therefore the prediction of logP has been examined countless times. One of the best performing models is to predict the logP using multiple methods and average the result. We have used those averaged predictions to develop a training-set which was able to distil the information present across the disparate logP methods into one single model. Read More

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December 2018

Reliability of effective arterial elastance using peripheral arterial pressure as surrogate for left ventricular end-systolic pressure.

J Clin Monit Comput 2018 Dec 14. Epub 2018 Dec 14.

Department Anaesthesia and Intensive Care Units, Humanitas Research Hospital, Humanitas University, Milan, Italy.

To compare the effective arterial elastance (Ea) obtained from the arterial pressure with Ea calculated from left-ventricular (LV) pressure-volume analysis. Experimental study. LV pressure-volume data was obtained with a conductance catheter and arterial pressures were measured via a fluid-filled catheter placed in the proximal aorta, femoral and radial arteries. Read More

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December 2018

A note on evolution of pressure and flow within an evaporating capillary bridge.

Eur Phys J E Soft Matter 2018 Dec 5;41(12):140. Epub 2018 Dec 5.

Duke University, Mechanical Engineering and Materials Science Dept., Durham, NC, USA.

Experiments with evaporation of capillary bridges between two glass spheres show that the bridge gorge radius decreases much faster than the contact radius, distorting the original constant mean curvature bridge shape. In addition, the Laplace pressure calculated from local principal curvatures exhibits high gradients along the bridge moving external surface, most commonly with a high suction near the triple phase contact and positive pressure near the gorge. The high suction results from a negative external curvature at contact. Read More

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December 2018

Microvascular obstruction extent predicts major adverse cardiovascular events in patients with acute myocardial infarction and preserved ejection fraction.

Eur Radiol 2018 Dec 14. Epub 2018 Dec 14.

Department of Radiological, Oncological and Pathological Sciences, Sapienza University of Rome "Sapienza", Viale Regina Elena 324, 00161, Rome, Italy.

Objectives: To investigate the prognostic role of early post-infarction cardiac magnetic resonance (CMR) on long-term risk stratification of ST segment elevation myocardial infarction (STEMI) patients with preserved left ventricular ejection fraction (LVEF).

Methods: Seventy-seven STEMI patients treated by primary percutaneous coronary intervention (PCI) and LVEF > 50% at CMR were included. The median time between STEMI and CMR was 5 days (IQR 2-8). Read More

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December 2018

In vitro-to-in vivo extrapolation (IVIVE) by PBTK modeling for animal-free risk assessment approaches of potential endocrine-disrupting compounds.

Arch Toxicol 2018 Dec 14. Epub 2018 Dec 14.

BASF SE, Experimental Toxicology and Ecology, Carl Bosch Straße 38, 67056, Ludwigshafen, Germany.

While in vitro testing is used to identify hazards of chemicals, nominal in vitro assay concentrations may misrepresent potential in vivo effects and do not provide dose-response data which can be used for a risk assessment. We used reverse dosimetry to compare in vitro effect concentrations-to-in vivo doses causing toxic effects related to endocrine disruption. Ten compounds (acetaminophen, bisphenol A, caffeine, 17α-ethinylestradiol, fenarimol, flutamide, genistein, ketoconazole, methyltestosterone, and trenbolone) have been tested in the yeast estrogen screening (YES) or yeast androgen-screening (YAS) assays for estrogen and androgen receptor binding, as well as the H295R assay (OECD test guideline no. Read More

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December 2018

How many cadherins do human endothelial cells express?

Cell Mol Life Sci 2018 Dec 14. Epub 2018 Dec 14.

Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, C/Nicolás Cabrera 1, Cantoblanco, 28049, Madrid, Spain.

The vasculature is the paradigm of a compartment generated by parallel cellular barriers that aims to transport oxygen, nutrients and immune cells in complex organisms. Vascular barrier dysfunction leads to fatal acute and chronic inflammatory diseases. The endothelial barrier lines the inner side of vessels and is the main regulator of vascular permeability. Read More

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December 2018

Adsorptive Removal of Tetracyclines and Fluoroquinolones Using Yak Dung Biochar.

Bull Environ Contam Toxicol 2018 Dec 14. Epub 2018 Dec 14.

Key Laboratory of Comprehensive and Highly Efficient Utilization of Salt Lake Resources, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences, Xining, 810008, Qinghai, China.

This study investigated the adsorptive removal of target fluoroquinolones and tetracyclines by biochar made by yak dung collected from the Qinghai-Tibet Plateau. Yak dung biochar pyrolyzed at 700 °C possessed surface area of 198.745 m g and multiple functional groups. Read More

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December 2018

Application of anisotropic NMR parameters to the confirmation of molecular structure.

Nat Protoc 2018 Dec 14. Epub 2018 Dec 14.

Structure Elucidation Group, Process and Analytical Research and Development, Merck Research Laboratories, Rahway, NJ, USA.

The use of anisotropic NMR data, such as residual dipolar couplings (RDCs) and residual chemical shift anisotropies (RCSAs), has emerged as a powerful technique for structural characterization of organic small molecules. RDCs typically report the relative orientations of different H-C bonds; RCSAs report the relative orientations of different carbon chemical shielding tensors and hence are more useful for proton-deficient molecules. This information is complementary to that obtained from conventional NMR data such as J couplings, isotropic chemical shifts, and nuclear Overhauser effects (NOEs)/rotational frame nuclear Overhauser effects (ROEs). Read More

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December 2018

Refining AML outcome prediction.

Leukemia 2018 Dec 14. Epub 2018 Dec 14.

Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.

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December 2018

Upregulation of MLK4 promotes migratory and invasive potential of breast cancer cells.

Oncogene 2018 Dec 14. Epub 2018 Dec 14.

Laboratory of Experimental Medicine, Centre of New Technologies, University of Warsaw, Warsaw, Poland.

Metastasis to distant organs is a major cause for solid cancer mortality, and the acquisition of migratory and invasive phenotype is a key factor in initiation of malignancy. In this study we investigated the contribution of Mixed-Lineage Kinase 4 (MLK4) to aggressive phenotype of breast cancer cells. Our TCGA cancer genomic data analysis revealed that amplification or mRNA upregulation of MLK4 occurred in 23% of invasive breast carcinoma cases. Read More

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December 2018

On the role of eye contact in gaze cueing.

Sci Rep 2018 Dec 14;8(1):17842. Epub 2018 Dec 14.

Istituto Italiano di Tecnologia, Social Cognition in Human-Robot Interaction, via Enrico Melen 83, 16152, Genova, Italy.

Most experimental protocols examining joint attention with the gaze cueing paradigm are "observational" and "offline", thereby not involving social interaction. We examined whether within a naturalistic online interaction, real-time eye contact influences the gaze cueing effect (GCE). We embedded gaze cueing in an interactive protocol with the iCub humanoid robot. Read More

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December 2018
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