26,893 results match your criteria European Journal of Biochemistry[Journal]


Structure of the core oligosaccharide of a rough-type lipopolysaccharide of Pseudomonas syringae pv. phaseolicola.

Eur J Biochem 2004 Dec;271(23-24):4968-77

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences Moscow, Russia.

The core structure of the lipopolysaccharide (LPS) isolated from a rough strain of the phytopathogenic bacterium Pseudomonas syringae pv. phaseolicola, GSPB 711, was investigated by sugar and methylation analyses, Fourier transform ion-cyclotron resonance ESI MS, and one- and two-dimensional 1H-, 13C- and 31P-NMR spectroscopy. Strong alkaline deacylation of the LPS resulted in two core-lipid A backbone undecasaccharide pentakisphosphates in the ratio approximately 2. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/j.1432-1033.2004.04467.x
Publisher Site
http://dx.doi.org/10.1111/j.1432-1033.2004.04467.xDOI Listing
December 2004
17 Reads

Monitoring ligand-mediated nuclear receptor-coregulator interactions by noncovalent mass spectrometry.

Eur J Biochem 2004 Dec;271(23-24):4958-67

Laboratoire de Spectrométrie de Masse Bio-Organique, CNRS UMR 7509, ECPM, Strasbourg, France.

Retinoid receptors are ligand-dependent transcription factors belonging to the nuclear receptor superfamily. Retinoic acid (RARalpha, beta, gamma) and retinoid X (RXRalpha, beta, gamma) receptors mediate the retinoid/rexinoid signal to the transcriptional machineries by interacting at the first level with coactivators or corepressors, which leads to the recruitment of enzymatically active noncovalent complexes at target gene promoters. It has been shown that the interaction of corepressors with nuclear receptors involves conserved LXXI/HIXXXI/L consensus sequences termed corepressor nuclear receptor (CoRNR) boxes. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/j.1432-1033.2004.04466.x
Publisher Site
http://dx.doi.org/10.1111/j.1432-1033.2004.04466.xDOI Listing
December 2004
32 Reads

Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy.

Eur J Biochem 2004 Dec;271(23-24):4950-7

Institute of Biochemistry & Biophysics, University of Tehran, Iran.

The NMR solution structures of NTX-1 (PDB code 1W6B and BMRB 6288), a long neurotoxin isolated from the venom of Naja naja oxiana, and the molecular dynamics simulation of these structures are reported. Calculations are based on 1114 NOEs, 19 hydrogen bonds, 19 dihedral angle restraints and secondary chemical shifts derived from 1H to 13C HSQC spectrum. Similar to other long neurotoxins, the three-finger like structure shows a double and a triple stranded beta-sheet as well as some flexible regions, particularly at the tip of loop II and the C-terminal tail. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04465.xDOI Listing
December 2004
16 Reads

Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure is alpha-D-Gal-(1-->3)-alpha-D-GalNAc.

Eur J Biochem 2004 Dec;271(23-24):4939-49

The Clayton Foundation Laboratories for Peptide Biology, The Salk Institute, La Jolla, CA, USA.

The 13-amino acid glycopeptide tx5a (Gla-Cys-Cys-Gla-Asp-Gly-Trp*-Cys-Cys-Thr*-Ala-Ala-Hyp-OH, where Trp* = 6-bromotryptophan and Thr* = Gal-GalNAc-threonine), isolated from Conus textile, causes hyperactivity and spasticity when injected intracerebral ventricularly into mice. It contains nine post-translationally modified residues: four cysteine residues, two gamma-carboxyglutamic acid residues, and one residue each of 6-bromotryptophan, 4-trans-hydroxyproline and glycosylated threonine. The chemical nature of each of these has been determined with the exception of the glycan linkage pattern on threonine and the stereochemistry of the 6-bromotryptophan residue. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/j.1432-1033.2004.04464.x
Publisher Site
http://dx.doi.org/10.1111/j.1432-1033.2004.04464.xDOI Listing
December 2004
17 Reads
3 Citations

Trans-splicing of a mutated glycosylasparaginase mRNA sequence by a group I ribozyme deficient in hydrolysis.

Eur J Biochem 2004 Dec;271(23-24):4932-8

Department of Molecular Biotechnology, RNA Research group, Institute of Medical Biology, University of Tromsø, Norway.

RNA reprogramming represents a new concept in correcting genetic defects at the RNA level. However, for the technique to be useful for therapy, the level of reprogramming must be appropriate. To improve the efficiency of group I ribozyme-mediated RNA reprogramming, when using the Tetrahymena ribozyme, regions complementary to the target RNA have previously been extended in length and accessible sites in the target RNAs have been identified. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04462.xDOI Listing
December 2004
24 Reads

Aptamers to Escherichia coli core RNA polymerase that sense its interaction with rifampicin, sigma-subunit and GreB.

Eur J Biochem 2004 Dec;271(23-24):4921-31

Public Health Research Institute, Newark, New Jersey, USA.

Bacterial RNA polymerase (RNAP) is the central enzyme of gene expression that is responsible for the synthesis of all types of cellular RNAs. The process of transcription is accompanied by complex structural rearrangements of RNAP. Despite the recent progress in structural studies of RNAP, detailed mechanisms of conformational changes of RNAP that occur at different stages of transcription remain unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04461.xDOI Listing
December 2004
26 Reads

Structure, expression and regulation of the cannabinoid receptor gene (CB1) in Huntington's disease transgenic mice.

Eur J Biochem 2004 Dec;271(23-24):4909-20

Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia, Canada.

Loss of cannabinoid receptors (CB1) occurs prior to neurodegeneration in Huntington's disease (HD). The levels and distribution of CB1 RNA were equivalent in 3-week-old mice regardless of genotype demonstrating that the specific factors and appropriate chromatin structure that lead to the transcription of CB1 were present in the striatum of young R6/2 and R6/1 transgenic HD mice. The expression of the mutant HD transgene led progressively to decreased steady-state levels of CB1 mRNA in neurons of the lateral striatum, which was dependent on the size of the CAG repeat and relative expression of the gene encoding mutant huntingtin (HD). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04460.xDOI Listing
December 2004
15 Reads

Brain succinic semialdehyde dehydrogenase. Reactions of sulfhydryl residues connected with catalytic activity.

Eur J Biochem 2004 Dec;271(23-24):4903-8

Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon, Korea.

Incubation of an NAD+-dependent succinic semialdehyde dehydrogenase from bovine brain with 4-dimethylaminoazobenzene-4-iodoacetamide (DABIA) resulted in a time-dependent loss of enzymatic activity. This inactivation followed pseudo first-order kinetics with a second-order rate constant of 168 m(-1).min(-1). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04459.xDOI Listing
December 2004
24 Reads

Interaction of an approximately 40 kDa protein from regenerating rat liver with the -148 to -124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver.

Eur J Biochem 2004 Dec;271(23-24):4892-902

Gene Regulation Laboratory, Center for Biotechnology, Jawaharlal Nehru University, New Delhi, India.

The c-jun belongs to the family of proto-oncogenes and encodes for the protein Jun, a component of transcription factor AP-1 involved in regulation of the expression of genes indispensable for cell proliferation and differentiation. While the role of c-jun in the regulation of such genes has been well examined, the regulation of c-jun in proliferating cells is not fully understood. We have earlier reported that the -148 to -124 region of c-jun is involved in the positive regulation of c-jun transcription, and interacts with a positive regulatory factor (rat liver jun regulatory protein; RLjunRP) present in rat liver. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04458.xDOI Listing
December 2004
17 Reads

Mechanistic studies on bovine cytosolic 5'-nucleotidase II, an enzyme belonging to the HAD superfamily.

Eur J Biochem 2004 Dec;271(23-24):4881-91

Dipartimento di Scienze del Farmaco, Università di Sassari, Italy.

Cytosolic 5'-nucleotidase/phosphotransferase specific for 6-hydroxypurine monophosphate derivatives (cN-II), belongs to a class of phosphohydrolases that act through the formation of an enzyme-phosphate intermediate. Sequence alignment with members of the P-type ATPases/L-2-haloacid dehalogenase superfamily identified three highly conserved motifs in cN-II and other cytosolic nucleotidases. Mutagenesis studies at specific amino acids occurring in cN-II conserved motifs were performed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04457.xDOI Listing
December 2004
13 Reads

Mercury(II) binding to metallothioneins. Variables governing the formation and structural features of the mammalian Hg-MT species.

Eur J Biochem 2004 Dec;271(23-24):4872-80

Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, Spain.

With the aim of extending our knowledge on the reaction pathways of Zn-metallothionein (MT) and apo-MT species in the presence of Hg(II), we monitored the titration of Zn7-MT, Zn4-alphaMT and Zn3-betaMT proteins, at pH 7 and 3, with either HgCl2 or Hg(ClO4)2 by CD and UV-vis spectroscopy. Detailed analysis of the optical data revealed that standard variables, such as the pH of the solution, the binding ability of the counter-ion (chloride or perchlorate), and the time elapsed between subsequent additions of Hg(II) to the protein, play a determinant role in the stoichiometry, stereochemistry and degree of folding of the Hg-MT species. Despite the fact that the effect of these variables is unquestionable, it is difficult to generalize. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/j.1432-1033.2004.04456.x
Publisher Site
http://dx.doi.org/10.1111/j.1432-1033.2004.04456.xDOI Listing
December 2004
13 Reads

Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains.

Eur J Biochem 2004 Dec;271(23-24):4865-71

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

ATP-dependent Lon proteases belong to the superfamily of AAA+ proteins. Until recently, the identity of the residues involved in their proteolytic active sites was not elucidated. However, the putative catalytic Ser-Lys dyad was recently suggested through sequence comparison of more than 100 Lon proteases from various sources. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04452.xDOI Listing
December 2004
18 Reads

Transcriptional responses to glucose at different glycolytic rates in Saccharomyces cerevisiae.

Eur J Biochem 2004 Dec;271(23-24):4855-64

Department of Chemistry and Bioscience-Molecular Biotechnology, Chalmers University of Technology, Göteborg, Sweden.

The addition of glucose to Saccharomyces cerevisiae cells causes reprogramming of gene expression. Glucose is sensed by membrane receptors as well as (so far elusive) intracellular sensing mechanisms. The availability of four yeast strains that display different hexose uptake capacities allowed us to study glucose-induced effects at different glycolytic rates. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04451.xDOI Listing
December 2004
7 Reads

Cloning, over-expression, purification and characterization of Plasmodium falciparum enolase.

Eur J Biochem 2004 Dec;271(23-24):4845-54

Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India.

We have cloned, over-expressed and purified enolase from Plasmodium falciparum strain NF54 in Escherichia coli in active form, as an N-terminal His6-tagged protein. The sequence of the cloned enolase from the NF54 strain is identical to that of strain 3D7 used in full genome sequencing. The recombinant enolase (r-Pfen) could be obtained in large quantities (approximately 50 mg per litre of culture) in a highly purified form (> 95%). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04450.xDOI Listing
December 2004
14 Reads

Methylthioadenosine phosphorylase from the archaeon Pyrococcus furiosus. Mechanism of the reaction and assignment of disulfide bonds.

Eur J Biochem 2004 Dec;271(23-24):4834-44

Dipartimento di Biochimica e Biofisica F. Cedrangolo, Seconda Università di Napoli, Naples, Italy.

The extremely heat-stable 5'-methylthioadenosine phosphorylase from the hyperthermophilic archaeon Pyrococcus furiosus was cloned, expressed to high levels in Escherichia coli, and purified to homogeneity by heat precipitation and affinity chromatography. The recombinant enzyme was subjected to a kinetic analysis including initial velocity and product inhibition studies. The reaction follows an ordered Bi-Bi mechanism and phosphate binding precedes nucleoside binding in the phosphorolytic direction. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/j.1432-1033.2004.04449.x
Publisher Site
http://dx.doi.org/10.1111/j.1432-1033.2004.04449.xDOI Listing
December 2004
20 Reads

Antimicrobial activity of histones from hemocytes of the Pacific white shrimp.

Eur J Biochem 2004 Dec;271(23-24):4825-33

Department of Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston, SC 29425, USA.

The role of vertebrate histone proteins or histone derived peptides as innate immune effectors has only recently been appreciated. In this study, high levels of core histone proteins H2A, H2B, H3 and H4 were found in hemocytes from the Pacific white shrimp, Litopenaeus vannamei. The proteins were identified by in-gel digestion, mass spectrometry analysis, and homology searching. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04448.xDOI Listing
December 2004
19 Reads

Solution structure of the active-centre mutant I14A of the histidine-containing phosphocarrier protein from Staphylococcus carnosus.

Eur J Biochem 2004 Dec;271(23-24):4815-24

Institute of Biophysics and Physical Biochemistry, University of Regensburg, Germany.

High-pressure NMR experiments performed on the histidine-containing phosphocarrier protein (HPr) from Staphylococcus carnosus have shown that residue Ile14, which is located in the active-centre loop, exhibits a peculiarly small pressure response. In contrast, the rest of the loop shows strong pressure effects as is expected for typical protein interaction sites. To elucidate the structural role of this residue, the mutant protein HPr(I14A), in which Ile14 is replaced by Ala, was produced and studied by solution NMR spectroscopy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04447.xDOI Listing
December 2004
8 Reads

pH dependence, substrate specificity and inhibition of human kynurenine aminotransferase I.

Eur J Biochem 2004 Dec;271(23-24):4804-14

Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL 61802, USA.

Human kynurenine aminotransferase I/glutamine transaminase K (hKAT-I) is an important multifunctional enzyme. This study systematically studies the substrates of hKAT-I and reassesses the effects of pH, Tris, amino acids and alpha-keto acids on the activity of the enzyme. The experiments were comprised of functional expression of the hKAT-I in an insect cell/baculovirus expression system, purification of its recombinant protein, and functional characterization of the purified enzyme. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04446.xDOI Listing
December 2004
34 Reads

Molecular identification of monomeric aspartate racemase from Bifidobacterium bifidum.

Eur J Biochem 2004 Dec;271(23-24):4798-803

Department of Bioresources Science, Kochi University, Nankoku, Kochi, Japan.

Bifidobacterium bifidum is a useful probiotic agent exhibiting health-promoting properties and contains d-aspartate as an essential component of the cross-linker moiety in the peptidoglycan. To help understand D-aspartate biosynthesis in B. bifidum NBRC 14252, aspartate racemase, which catalyzes the racemization of D- and L-aspartate, was purified to homogeneity and characterized. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04445.xDOI Listing
December 2004
14 Reads

Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae.

Eur J Biochem 2004 Dec;271(23-24):4788-97

Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark.

Saccharomyces cerevisiae is a widely used host in the production of therapeutic peptides and proteins. Here we report the identification of a novel endoprotease in S. cerevisiae. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04443.xDOI Listing
December 2004
13 Reads

Trigger factor interacts with the signal peptide of nascent Tat substrates but does not play a critical role in Tat-mediated export.

Eur J Biochem 2004 Dec;271(23-24):4779-87

Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, Amsterdam, The Netherlands.

Twin-arginine translocation (Tat)-mediated protein transport across the bacterial cytoplasmic membrane occurs only after synthesis and folding of the substrate protein that contains a signal peptide with a characteristic twin-arginine motif. This implies that premature contact between the Tat signal peptide and the Tat translocon in the membrane must be prevented. We used site-specific photo-crosslinking to demonstrate that the signal peptide of nascent Tat proteins is in close proximity to the chaperone and peptidyl-prolyl isomerase trigger factor (TF). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04442.xDOI Listing
December 2004
21 Reads

Escherichia coli cyclopropane fatty acid synthase.

Eur J Biochem 2004 Dec;271(23-24):4769-78

Laboratoire de Chimie Organique Biologique, UMR7613 CNRS, Université Pierre et Marie Curie, Paris, France.

Escherichia coli fatty acid cyclopropane synthase (CFAS) was overproduced and purified as a His6-tagged protein. This recombinant enzyme is as active as the native enzyme with a Km of 90 microm for S-AdoMet and a specific activity of 5 x 10(-2) micromol.min(-1). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04441.xDOI Listing
December 2004
22 Reads

Universal positions in globular proteins.

Eur J Biochem 2004 Dec;271(23-24):4762-8

Laboratory of Genetics, Agricultural University of Athens, Greece.

The description of globular protein structures as an ensemble of contiguous 'closed loops' or 'tightened end fragments' reveals fold elements crucial for the formation of stable structures and for navigating the very process of protein folding. These are the ends of the loops, which are spatially close to each other but are situated apart in the polypeptide chain by 25-30 residues. They also correlate with the locations of highly conserved hydrophobic residues (referred to as topohydrophobic), in a structural alignment of the members of a protein family. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04440.xDOI Listing
December 2004
10 Reads

Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus.

Eur J Biochem 2004 Dec;271(23-24):4753-61

Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Cuernavaca, Mexico.

A peptide called phaiodotoxin was isolated from the venom of the scorpion Anuroctonus phaiodactylus. It is lethal to crickets, but non toxic to mice at the doses assayed. It has 72 amino acid residues, with a molecular mass of 7971 atomic mass units. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04439.xDOI Listing
December 2004
16 Reads

Radical-induced oxidation of metformin.

Eur J Biochem 2004 Dec;271(23-24):4745-52

Laboratoire de Chimie Physique UMR 8601-CNRS, Université Paris 5, France.

Metformin (1,1-dimethylbiguanide) is an antihyperglycaemic drug used to normalize glucose concentrations in type 2 diabetes. Furthermore, antioxidant benefits have been reported in diabetic patients treated with metformin. This work was aimed at studying the scavenging capacity of this drug against reactive oxygen species (ROS) like *OH and (O2*-)-free radicals. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04438.xDOI Listing
December 2004
9 Reads

Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation.

Eur J Biochem 2004 Dec;271(23-24):4737-44

Laboratoire Génétique et Membranes, Département Biologie Cellulaire, Institut Jacques Monod, UMR 7592 CNRS, Universités Paris VI-VII, Paris, France.

The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the chloroplast of Chlamydomonas reinhardtii is part of a complex that also includes phosphoribulokinase (PRK) and CP12. We identified two residues of GAPDH involved in protein-protein interactions in this complex, by changing residues K128 and R197 into A or E. K128A/E mutants had a Km for NADH that was twice that of the wild type and a lower catalytic constant, whatever the cofactor. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/j.1432-1033.2004.04437.x
Publisher Site
http://dx.doi.org/10.1111/j.1432-1033.2004.04437.xDOI Listing
December 2004
16 Reads

Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus.

Eur J Biochem 2004 Dec;271(23-24):4725-36

Institute of Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China.

The structure and membrane interaction of the internal fusion peptide (IFP) fragment of the avian sarcoma and leucosis virus (ASLV) envelope glycoprotein was studied by an array of biophysical methods. The peptide was found to induce lipid mixing of vesicles more strongly than the fusion peptide derived from the N-terminal fusion peptide of influenza virus (HA2-FP). It was observed that the helical structure was enhanced in association with the model membranes, particularly in the N-terminal portion of the peptide. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04436.xDOI Listing
December 2004
24 Reads

Complex transcriptional and translational regulation of iPLAgamma resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization.

Eur J Biochem 2004 Dec;271(23-24):4709-24

Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.

Membrane-associated calcium-independent phospholipase A2gamma (iPLA2gamma) contains four potential in-frame methionine start sites (Mancuso, D.J. Jenkins, C. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04435.xDOI Listing
December 2004
26 Reads

1,5-diamino-2-pentyne is both a substrate and inactivator of plant copper amine oxidases.

Eur J Biochem 2004 Dec;271(23-24):4696-708

Department of Biochemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic.

1,5-diamino-2-pentyne (DAPY) was found to be a weak substrate of grass pea (Lathyrus sativus, GPAO) and sainfoin (Onobrychis viciifolia, OVAO) amine oxidases. Prolonged incubations, however, resulted in irreversible inhibition of both enzymes. For GPAO and OVAO, rates of inactivation of 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04434.xDOI Listing
December 2004
15 Reads

The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB.

Eur J Biochem 2004 Dec;271(23-24):4685-95

Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada.

In an attempt to isolate lipopolysaccharide from Spirochaeta aurantia, Darveau-Hancock extraction of the cell mass was performed. While no lipopolysaccharide was found, two carbohydrate-containing compounds were detected. They were resolved by size-exclusion chromatography into high molecular mass (LGLA) and low molecular mass (LGLB) fractions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04433.xDOI Listing
December 2004
12 Reads

A novel gamma-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1.

Eur J Biochem 2004 Dec;271(23-24):4677-84

Institute of Biochemistry and Molecular Biology, University of Freiburg, Freiburg, Germany.

Nicotine catabolism, linked in Arthrobacter nicotinovorans to the presence of the megaplasmid pAO1, leads to the formation of gamma-N-methylaminobutyrate from the pyrrolidine ring of the alkaloid. Until now the metabolic fate of gamma-N-methylaminobutyrate has been unknown. pAO1 carries a cluster of ORFs with similarity to sarcosine and dimethylglycine dehydrogenases and oxidases, to the bifunctional enzyme methylenetetrahydrofolate dehydrogenase/cyclohydrolase and to formyltetrahydrofolate deformylase. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/j.1432-1033.2004.04432.x
Publisher Site
http://dx.doi.org/10.1111/j.1432-1033.2004.04432.xDOI Listing
December 2004
10 Reads

Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone.

Eur J Biochem 2004 Dec;271(23-24):4666-76

Department of Biomedical Sciences, University of Teramo, Italy.

We have shown recently that in human T lymphocytes, leptin stimulates activity and expression of the endocannabinoid-degrading enzyme fatty acid amide hydrolase (FAAH), through STAT3 (signal transducer and activator of transcription 3) and its CRE (cAMP response element)-like transcriptional target in the FAAH promoter [Maccarrone, M., Di Rienzo, M., Finazzi-Agro, A. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/j.1432-1033.2004.04427.x
Publisher Site
http://dx.doi.org/10.1111/j.1432-1033.2004.04427.xDOI Listing
December 2004
14 Reads

Stoichiometry of LHCI antenna polypeptides and characterization of gap and linker pigments in higher plants Photosystem I.

Eur J Biochem 2004 Dec;271(23-24):4659-65

Dipartimento Scientifico e Tecnologico, Università di Verona, Verona, Italy.

We report on the results obtained by measuring the stoichiometry of antenna polypeptides in Photosystem I (PSI) from Arabidopsis thaliana. This analysis was performed by quantification of Coomassie blue binding to individual LHCI polypeptides, fractionation by SDS/PAGE, and by the use of recombinant light harvesting complex of Photosystem I (Lhca) holoproteins as a standard reference. Our results show that a single copy of each Lhca1-4 polypeptide is present in Photosystem I. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/j.1432-1033.2004.04426.x
Publisher Site
http://dx.doi.org/10.1111/j.1432-1033.2004.04426.xDOI Listing
December 2004
19 Reads

Regulation of the expression and subcellular localization of the taurine transporter TauT in mouse NIH3T3 fibroblasts.

Eur J Biochem 2004 Dec;271(23-24):4646-58

The August Krogh Institute, Biochemical Department, Universitetsparken 13, Copenhagen, Denmark.

The cellular level of the organic osmolyte taurine is a balance between active uptake and passive leak via a volume sensitive pathway. Here, we demonstrate that NIH3T3 mouse fibroblasts express a saturable, high affinity taurine transporter (TauT, Km = 18 microm), and that taurine uptake via TauT is a Na+- and Cl(-)-dependent process with an apparent 2.5 : 1 : 1 Na+/Cl-/taurine stoichiometry. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04420.xDOI Listing
December 2004
14 Reads

Kinetic analysis of zymogen autoactivation in the presence of a reversible inhibitor.

Eur J Biochem 2004 Dec;271(23-24):4638-45

National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing, PR China.

Limited proteolysis is a highly specific irreversible process, which can serve to initiate physiological function by converting a precursor protein into a biologically active form. When the activating enzyme and the activated enzyme coincide, the process is an autocatalytic zymogen activation (i.e. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04416.xDOI Listing
December 2004
9 Reads

NARG2 encodes a novel nuclear protein with (S/T)PXX motifs that is expressed during development.

Eur J Biochem 2004 Dec;271(23-24):4629-37

Department of Cell Biology and Anatomy, LSU Health Sciences Center, New Orleans, LA 70112, USA.

We previously identified a partial expressed sequence tag clone corresponding to NARG2 in a screen for genes that are expressed in developing neurons and misexpressed in transgenic mice that lack functional N-methyl-d-aspartate receptors. Here we report the first characterization of the mouse and human NARG2 genes, cDNAs and the proteins that they encode. Mouse and human NARG2 consist of 988 and 982 amino acids, respectively, and share 74% identity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04414.xDOI Listing
December 2004
6 Reads

Weapons of STAT destruction. Interferon evasion by paramyxovirus V protein.

Authors:
Curt M Horvath

Eur J Biochem 2004 Dec;271(23-24):4621-8

Department of Medicine, Evanston Northwestern Healthcare Research Institute, Northwestern University, Evanston, IL, USA.

The signal transducer and activator of transcription (STAT) family of proteins function to activate gene transcription downstream of myriad cytokine and growth factor signals. The prototype STAT proteins, STAT1 and STAT2, are required for innate and adaptive antimicrobial immune responses that result from interferon signal transduction. While many viruses have evolved the ability to avoid these antiviral cytokines, the Paramyxoviruses are distinct in their abilities to interfere directly with STAT proteins. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04425.xDOI Listing
December 2004
17 Reads

Regulation of STAT signalling by proteolytic processing.

Eur J Biochem 2004 Dec;271(23-24):4613-20

Peter Gorer Department of Immunobiology, Programme in Infection and Immunity, King's College, London, UK.

Interaction of cytokines with their cognate receptors leads to the activation of latent transcription factors, the signal transducer and activator of transcription (STAT) proteins. Numerous studies have identified the critical roles played by STAT proteins in regulating cell proliferation, differentiation and survival. Consequently, the activity of STAT proteins is negatively regulated by a variety of different mechanisms, which include alternative splicing, covalent modifications, protein-protein interactions with negative regulatory proteins and proteolytic processing by proteases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04424.xDOI Listing
December 2004
19 Reads

Nucleocytoplasmic shuttling of STAT transcription factors.

Eur J Biochem 2004 Dec;271(23-24):4606-12

Abteilung Zelluläre Signalverarbeitung, Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Freie Universität, Berlin, Germany.

The signal transducer and activator of transcription (STAT) proteins have initially been described as cytoplasmic proteins that enter the nucleus only after cytokine treatment of cells. Contrary to this assumption, it was demonstrated that STATs are constantly shuttling between nucleus and cytoplasm irrespective of cytokine stimulation. This happens both via carrier-dependent as well as carrier-independent transportation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04423.xDOI Listing
December 2004
7 Reads

Symmetric fluoro-substituted diol-based HIV protease inhibitors. Ortho-fluorinated and meta-fluorinated P1/P1'-benzyloxy side groups significantly improve the antiviral activity and preserve binding efficacy.

Eur J Biochem 2004 Nov;271(22):4594-602

Department of Cell and Molecular Biology, BMC, Uppsala University, Sweden.

HIV-1 protease is a pivotal enzyme in the later stages of the viral life cycle which is responsible for the processing and maturation of the virus particle into an infectious virion. As such, HIV-1 protease has become an important target for the treatment of AIDS, and efficient drugs have been developed. However, negative side effects and fast emerging resistance to the current drugs have necessitated the development of novel chemical entities in order to exploit different pharmacokinetic properties as well as new interaction patterns. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04431.xDOI Listing
November 2004
10 Reads

Inhibition of pea ferredoxin-NADP(H) reductase by Zn-ferrocyanide.

Eur J Biochem 2004 Nov;271(22):4582-93

Molecular Biology Division, IBR (Instituto de Biología Molecular y Celular de Rosario), Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina.

Ferredoxin-NADP(H) reductases (FNRs) represent a prototype of enzymes involved in numerous metabolic pathways. We found that pea FNR ferricyanide diaphorase activity was inhibited by Zn2+ (Ki 1.57 microM). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04430.xDOI Listing
November 2004
14 Reads
1 Citation

Regulation of the actin-myosin interaction by titin.

Eur J Biochem 2004 Nov;271(22):4572-81

CRBM-CNRS, Montpellier, France.

Titin is known to interact with actin thin filaments within the I-band region of striated muscle sarcomeres. In this study, we have used a titin fragment of 800 kDa (T800) purified from striated skeletal muscle to measure the effect of this interaction on the functional properties of the actin-myosin complex. MALDI-TOF MS revealed that T800 contains the entire titin PEVK (Pro, Glu, Val, Lys-rich) domain. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04429.xDOI Listing
November 2004
26 Reads

The mechanism of alpha-proton isotope exchange in amino acids catalysed by tyrosine phenol-lyase. What is the role of quinonoid intermediates?

Eur J Biochem 2004 Nov;271(22):4565-71

Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, Russia.

To shed light on the mechanism of isotopic exchange of alpha-protons in amino acids catalyzed by pyridoxal phosphate (PLP)-dependent enzymes, we studied the kinetics of quinonoid intermediate formation for the reactions of tyrosine phenol-lyase with L-phenylalanine, L-methionine, and their alpha-deuterated analogues in D2O, and we compared the results with the rates of the isotopic exchange under the same conditions. We have found that, in the L-phenylalanine reaction, the internal return of the alpha-proton is operative, and allowing for its effect, the exchange rate is accounted for satisfactorily. Surprisingly, for the reaction with L-methionine, the enzymatic isotope exchange went much faster than might be predicted from the kinetic data for quinonoid intermediate formation. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/j.1432-1033.2004.04428.x
Publisher Site
http://dx.doi.org/10.1111/j.1432-1033.2004.04428.xDOI Listing
November 2004
29 Reads

Age-related impairment of mitochondrial matrix aconitase and ATP-stimulated protease in rat liver and heart.

Eur J Biochem 2004 Nov;271(22):4559-64

Laboratoire de Biologie et Biochimie Cellulaire du Vieillissement, Université Paris 7-Denis Diderot, Paris, France.

Mitochondrial matrix proteins are sensitive to oxidative inactivation, and oxidized proteins are known to accumulate during ageing. The Lon protease is believed to play an important role in the degradation of oxidized matrix proteins such as oxidized aconitase. We reported previously that an age-related accumulation of altered proteins occurs in the liver matrix of rats and that the ATP-stimulated proteolytic activity, referred as to Lon-like protease activity, decreases considerably in 27 month-old rats, whereas no concomitant changes in the levels of Lon protein expression occur in the liver. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/j.1432-1033.2004.04422.x
Publisher Site
http://dx.doi.org/10.1111/j.1432-1033.2004.04422.xDOI Listing
November 2004
9 Reads

Suppression of heat- and polyglutamine-induced cytotoxicity by nonsteroidal anti-inflammatory drugs.

Eur J Biochem 2004 Nov;271(22):4552-8

Department of Biochemistry, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto, Japan.

We have shown that sodium salicylate activates the heat shock promoter and induces the expression of heat shock proteins (hsps), with a concomitant increase in the thermotolerance of cells. To determine whether these effects are generally displayed by nonsteroidal anti-inflammatory drugs (NSAIDs), we examined the effects of a cyclooxygenase inhibitor, indomethacin, and a lipoxygenase inhibitor, nordihydroguaiaretic acid. Both inhibitors up-regulated the hsp promoter at 37 degrees C through the activation of heat shock factors, and increased cellular levels of hsps in mammalian cells, although the degree of the expression of hsps and thermotolerance of cells differed depending on the drugs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04419.xDOI Listing
November 2004
12 Reads

Structural determination of the polar glycoglycerolipids from thermophilic bacteria Meiothermus taiwanensis.

Eur J Biochem 2004 Nov;271(22):4545-51

Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.

The polar glycolipids were isolated from the thermophilic bacteria Meiothermus taiwanensis ATCC BAA-400 by ethanol extraction and purified by Sephadex LH-20 and silica gel column chromatography. The fatty acid composition of O-acyl groups in the glycolipids was obtained by gas chromatography mass spectroscopy analysis on their methyl esters derived from methanolysis and was made mainly of C(15:0) (34.0%) and C(17:0) (42. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04415.xDOI Listing
November 2004
13 Reads

Functional dissection of a small anaerobically induced bZIP transcription factor from tomato.

Eur J Biochem 2004 Nov;271(22):4534-44

Institut für Genetik, Technische Universität Braunschweig, Germany.

A small anaerobically induced tomato transcription factor was isolated from a subtractive library. This factor, designated ABZ1 (anaerobic basic leucine zipper), is anaerobically induced in fruits, leaves and roots and encodes a nuclear localized protein. ABZ1 shares close structural and sequence homology with the S-family of small basic leucine zipper (bZIP) transcription factors that are implicated in stress response. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04413.xDOI Listing
November 2004
13 Reads

Surface exposed amino acid differences between mesophilic and thermophilic phosphoribosyl diphosphate synthase.

Eur J Biochem 2004 Nov;271(22):4526-33

Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, Denmark.

The amino acid sequence of 5-phospho-alpha-D-ribosyl 1-diphosphate synthase from the thermophile Bacillus caldolyticus is 81% identical to the amino acid sequence of 5-phospho-alpha-D-ribosyl 1-diphosphate synthase from the mesophile Bacillus subtilis. Nevertheless the enzyme from the two organisms possesses very different thermal properties. The B. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/j.1432-1033.2004.04412.x
Publisher Site
http://dx.doi.org/10.1111/j.1432-1033.2004.04412.xDOI Listing
November 2004
13 Reads

Crystal structure of heme oxygenase-1 from cyanobacterium Synechocystis sp. PCC 6803 in complex with heme.

Eur J Biochem 2004 Nov;271(22):4517-25

Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan.

Heme oxygenase (HO) catalyzes the oxidative degradation of heme utilizing molecular oxygen and reducing equivalents. In photosynthetic organisms, HO functions in the biosynthesis of such open-chain tetrapyrroles as phyto-chromobilin and phycobilins, which are involved in the signal transduction for light responses and light harvesting for photosynthesis, respectively. We have determined the first crystal structure of a HO-1 from a photosynthetic organism, Synechocystis sp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04411.xDOI Listing
November 2004
16 Reads

Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions.

Eur J Biochem 2004 Nov;271(22):4507-16

Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada.

Aeromonas salmonicida is a pathogenic aquatic bacterium and the causal agent of furunculosis in salmon. In the course of this study, it was found that when grown in vitro on tryptic soy agar, A. salmonicida strain 80204-1 produced a capsular polysaccharide with the identical structure to that of the lipopolysaccharide O-chain polysaccharide. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1432-1033.2004.04410.xDOI Listing
November 2004
25 Reads