2,395 results match your criteria Eukaryotic Cell[Journal]


Correction for Liang et al., A Novel Function for Hog1 Stress-Activated Protein Kinase in Controlling White-Opaque Switching and Mating in Candida albicans.

Eukaryot Cell 2015 Dec;14(12):iii-iv

Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei, Taiwan.

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http://dx.doi.org/10.1128/EC.00005-16DOI Listing
December 2015
4 Reads

Retraction for Chavez-Dozal et al. Functional Analysis of the Exocyst Subunit Sec15 in Candida albicans.

Eukaryot Cell 2015 Dec;14(12):ii

Section of Infectious Diseases, New Mexico VA Healthcare System, Albuquerque, New Mexico, USA Division of Infectious Diseases, University of New Mexico Health Science Center, Albuquerque, New Mexico, USA.

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http://ec.asm.org/lookup/doi/10.1128/EC.00002-16
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http://dx.doi.org/10.1128/EC.00002-16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025947PMC
December 2015
4 Reads

Retraction for Chavez-Dozal et al., The Candida albicans Exocyst Subunit Sec6 Contributes to Cell Wall Integrity and Is a Determinant of Hyphal Branching.

Eukaryot Cell 2015 Dec;14(12)

Section of Infectious Diseases, New Mexico VA Healthcare System, Albuquerque, New Mexico, USA Division of Infectious Diseases, University of New Mexico Health Science Center, Albuquerque, New Mexico, USA.

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http://ec.asm.org/lookup/doi/10.1128/EC.00001-16
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http://dx.doi.org/10.1128/EC.00001-16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025946PMC
December 2015
10 Reads

The New Shape of EC.

Authors:
Aaron P Mitchell

Eukaryot Cell 2015 Dec;14(12):1151-2

Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA

The journal Eukaryotic Cell has served the eukaryotic microbiology community since 2002. It will continue to do so as it merges into the new broad-scope open-access journal mSphere in 2016. Read More

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http://dx.doi.org/10.1128/EC.00139-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664875PMC
December 2015
12 Reads

Erratum for Yamamoto et al., Novel 44-Kilodalton Subunit of Axonemal Dynein Conserved from to Mammals.

Eukaryot Cell 2015 Dec 15;14(12). Epub 2017 Mar 15.

Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033.

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http://dx.doi.org/10.1128/EC.00001-17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362130PMC
December 2015
5 Reads

N-Terminal Presequence-Independent Import of Phosphofructokinase into Hydrogenosomes of Trichomonas vaginalis.

Eukaryot Cell 2015 Dec 16;14(12):1264-75. Epub 2015 Oct 16.

Department of Parasitology, Charles University in Prague, Faculty of Science, Prague, Czech Republic

Mitochondrial evolution entailed the origin of protein import machinery that allows nuclear-encoded proteins to be targeted to the organelle, as well as the origin of cleavable N-terminal targeting sequences (NTS) that allow efficient sorting and import of matrix proteins. In hydrogenosomes and mitosomes, reduced forms of mitochondria with reduced proteomes, NTS-independent targeting of matrix proteins is known. Here, we studied the cellular localization of two glycolytic enzymes in the anaerobic pathogen Trichomonas vaginalis: PPi-dependent phosphofructokinase (TvPPi-PFK), which is the main glycolytic PFK activity of the protist, and ATP-dependent PFK (TvATP-PFK), the function of which is less clear. Read More

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http://dx.doi.org/10.1128/EC.00104-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664869PMC
December 2015
7 Reads

Functional Analysis of the Exocyst Subunit Sec15 in Candida albicans.

Eukaryot Cell 2015 Dec 9;14(12):1228-39. Epub 2015 Oct 9.

Section of Infectious Diseases, New Mexico VA Healthcare System, Albuquerque, New Mexico, USA Division of Infectious Diseases, University of New Mexico Health Science Center, Albuquerque, New Mexico, USA

In prior studies of exocyst-mediated late secretion in Candida albicans, we have determined that Sec6 contributes to cell wall integrity, secretion, and filamentation. A conditional mutant lacking SEC6 expression exhibits markedly reduced lateral hyphal branching. In addition, lack of the related t-SNAREs Sso2 and Sec9 also leads to defects in secretion and filamentation. Read More

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http://dx.doi.org/10.1128/EC.00147-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664874PMC
December 2015
19 Reads

Depletion of UBC9 Causes Nuclear Defects during the Vegetative and Sexual Life Cycles in Tetrahymena thermophila.

Eukaryot Cell 2015 Dec 9;14(12):1240-52. Epub 2015 Oct 9.

Department of Biochemistry, Purdue University, West Lafayette, Indiana, USA

Ubc9p is the sole E2-conjugating enzyme for SUMOylation, and its proper function is required for regulating key nuclear events such as transcription, DNA repair, and mitosis. In Tetrahymena thermophila, the genome is separated into a diploid germ line micronucleus (MIC) that divides by mitosis and a polyploid somatic macronucleus (MAC) that divides amitotically. This unusual nuclear organization provides novel opportunities for the study of SUMOylation and Ubc9p function. Read More

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http://dx.doi.org/10.1128/EC.00115-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664876PMC
December 2015
5 Reads

Characterization of a Novel Prevacuolar Compartment in Neurospora crassa.

Eukaryot Cell 2015 Dec 9;14(12):1253-63. Epub 2015 Oct 9.

Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, California, USA.

Using confocal microscopy, we observed ring-like organelles, similar in size to nuclei, in the hyphal tip of the filamentous fungus Neurospora crassa. These organelles contained a subset of vacuolar proteins. We hypothesize that they are novel prevacuolar compartments (PVCs). Read More

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http://dx.doi.org/10.1128/EC.00128-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664880PMC
December 2015
10 Reads

Virulence-Associated Enzymes of Cryptococcus neoformans.

Eukaryot Cell 2015 Dec 9;14(12):1173-85. Epub 2015 Oct 9.

Department of Microbiology and Immunology, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York, USA Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA

Enzymes play key roles in fungal pathogenesis. Manipulation of enzyme expression or activity can significantly alter the infection process, and enzyme expression profiles can be a hallmark of disease. Hence, enzymes are worthy targets for better understanding pathogenesis and identifying new options for combatting fungal infections. Read More

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http://ec.asm.org/lookup/doi/10.1128/EC.00103-15
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http://dx.doi.org/10.1128/EC.00103-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664877PMC
December 2015
12 Reads

Adaptations of the Secretome of Candida albicans in Response to Host-Related Environmental Conditions.

Eukaryot Cell 2015 Dec 9;14(12):1165-72. Epub 2015 Oct 9.

Department of Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.

The wall proteome and the secretome of the fungal pathogen Candida albicans help it to thrive in multiple niches of the human body. Mass spectrometry has allowed researchers to study the dynamics of both subproteomes. Here, we discuss some major responses of the secretome to host-related environmental conditions. Read More

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http://dx.doi.org/10.1128/EC.00142-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664879PMC
December 2015
4 Reads

Yeast Integral Membrane Proteins Apq12, Brl1, and Brr6 Form a Complex Important for Regulation of Membrane Homeostasis and Nuclear Pore Complex Biogenesis.

Eukaryot Cell 2015 Dec 2;14(12):1217-27. Epub 2015 Oct 2.

Department of Biology, University of Fribourg, Fribourg, Switzerland

Proper functioning of intracellular membranes is critical for many cellular processes. A key feature of membranes is their ability to adapt to changes in environmental conditions by adjusting their composition so as to maintain constant biophysical properties, including fluidity and flexibility. Similar changes in the biophysical properties of membranes likely occur when intracellular processes, such as vesicle formation and fusion, require dramatic changes in membrane curvature. Read More

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http://ec.asm.org/lookup/doi/10.1128/EC.00101-15
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http://dx.doi.org/10.1128/EC.00101-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664871PMC
December 2015
14 Reads

Saccharomyces cerevisiae Is Dependent on Vesicular Traffic between the Golgi Apparatus and the Vacuole When Inositolphosphorylceramide Synthase Aur1 Is Inactivated.

Eukaryot Cell 2015 Dec 2;14(12):1203-16. Epub 2015 Oct 2.

Division of Biochemistry, Department of Biology, University of Fribourg, Fribourg, Switzerland

Inositolphosphorylceramide (IPC) and its mannosylated derivatives are the only complex sphingolipids of yeast. Their synthesis can be reduced by aureobasidin A (AbA), which specifically inhibits the IPC synthase Aur1. AbA reportedly, by diminishing IPC levels, causes endoplasmic reticulum (ER) stress, an increase in cytosolic calcium, reactive oxygen production, and mitochondrial damage leading to apoptosis. Read More

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http://dx.doi.org/10.1128/EC.00117-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664882PMC
December 2015
11 Reads

Comparison of Switching and Biofilm Formation between MTL-Homozygous Strains of Candida albicans and Candida dubliniensis.

Eukaryot Cell 2015 Dec 2;14(12):1186-202. Epub 2015 Oct 2.

The Developmental Studies Hybridoma Bank, Department of Biological Sciences, The University of Iowa, Iowa City, Iowa, USA

Candida albicans and Candida dubliniensis are highly related species that share the same main developmental programs. In C. albicans, it has been demonstrated that the biofilms formed by strains heterozygous and homozygous at the mating type locus (MTL) differ functionally, but studies rarely identify the MTL configuration. Read More

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http://dx.doi.org/10.1128/EC.00146-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664870PMC
December 2015
5 Reads

The ABCs of Candida albicans Multidrug Transporter Cdr1.

Eukaryot Cell 2015 Dec 25;14(12):1154-64. Epub 2015 Sep 25.

Membrane Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.

In the light of multidrug resistance (MDR) among pathogenic microbes and cancer cells, membrane transporters have gained profound clinical significance. Chemotherapeutic failure, by far, has been attributed mainly to the robust and diverse array of these proteins, which are omnipresent in every stratum of the living world. Candida albicans, one of the major fungal pathogens affecting immunocompromised patients, also develops MDR during the course of chemotherapy. Read More

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http://dx.doi.org/10.1128/EC.00137-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4664872PMC
December 2015
3 Reads

Evidence that the Entamoeba histolytica Mitochondrial Carrier Family Links Mitosomal and Cytosolic Pathways through Exchange of 3'-Phosphoadenosine 5'-Phosphosulfate and ATP.

Eukaryot Cell 2015 Nov 18;14(11):1144-50. Epub 2015 Sep 18.

Department of Parasitology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan

Entamoeba histolytica, a microaerophilic protozoan parasite, possesses mitosomes. Mitosomes are mitochondrion-related organelles that have largely lost typical mitochondrial functions, such as those involved in the tricarboxylic acid cycle and oxidative phosphorylation. The biological roles of Entamoeba mitosomes have been a long-standing enigma. Read More

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http://dx.doi.org/10.1128/EC.00130-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621310PMC
November 2015
9 Reads

The Cch1-Mid1 High-Affinity Calcium Channel Contributes to the Virulence of Cryptococcus neoformans by Mitigating Oxidative Stress.

Eukaryot Cell 2015 Nov 18;14(11):1135-43. Epub 2015 Sep 18.

Department of Pharmacology, School of Medicine, University of California, Genome and Biomedical Sciences Facility, Davis, California, USA

Pathogenic fungi have developed mechanisms to cope with stresses imposed by hosts. For Cryptococcus spp., this implies active defense mechanisms that attenuate and ultimately overcome the onslaught of oxidative stresses in macrophages. Read More

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http://dx.doi.org/10.1128/EC.00100-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621313PMC
November 2015
6 Reads

pH Regulates White-Opaque Switching and Sexual Mating in Candida albicans.

Eukaryot Cell 2015 Nov 4;14(11):1127-34. Epub 2015 Sep 4.

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

As a successful commensal and pathogen of humans, Candida albicans encounters a wide range of environmental conditions. Among them, ambient pH, which changes frequently and affects many biological processes in this species, is an important factor, and the ability to adapt to pH changes is tightly linked with pathogenesis and morphogenesis. In this study, we report that pH has a profound effect on white-opaque switching and sexual mating in C. Read More

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http://dx.doi.org/10.1128/EC.00123-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621312PMC
November 2015
2 Reads

Function and Regulation of Cph2 in Candida albicans.

Eukaryot Cell 2015 Nov 4;14(11):1114-26. Epub 2015 Sep 4.

Department of Biological Chemistry, University of California, Irvine, California, USA Institute for Genomics and Bioinformatics, University of California, Irvine, California, USA

Candida albicans is associated with humans as both a harmless commensal organism and a pathogen. Cph2 is a transcription factor whose DNA binding domain is similar to that of mammalian sterol response element binding proteins (SREBPs). SREBPs are master regulators of cellular cholesterol levels and are highly conserved from fungi to mammals. Read More

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http://dx.doi.org/10.1128/EC.00102-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621314PMC
November 2015
21 Reads

A Novel Type Pathway-Specific Regulator and Dynamic Genome Environments of a Solanapyrone Biosynthesis Gene Cluster in the Fungus Ascochyta rabiei.

Eukaryot Cell 2015 Nov 4;14(11):1102-13. Epub 2015 Sep 4.

Department of Plant Pathology, Washington State University, Pullman, Washington, USA USDA-ARS Grain Legume Genetics and Physiology Research Unit, Pullman, Washington, USA

Secondary metabolite genes are often clustered together and situated in particular genomic regions, like the subtelomere, that can facilitate niche adaptation in fungi. Solanapyrones are toxic secondary metabolites produced by fungi occupying different ecological niches. Full-genome sequencing of the ascomycete Ascochyta rabiei revealed a solanapyrone biosynthesis gene cluster embedded in an AT-rich region proximal to a telomere end and surrounded by Tc1/Mariner-type transposable elements. Read More

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http://dx.doi.org/10.1128/EC.00084-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621316PMC
November 2015
10 Reads

Unfolded Protein Response Pathways in Bloodstream-Form Trypanosoma brucei?

Eukaryot Cell 2015 Nov 28;14(11):1094-101. Epub 2015 Aug 28.

Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York, USA

The unfolded protein response (UPR) is a stress mechanism to cope with misfolded proteins in the early secretory pathway, the hallmark being transcriptional upregulation of endoplasmic reticulum (ER) molecular chaperones such as BiP and protein disulfide isomerase. Despite the lack of transcriptional regulation and the absence of the classical UPR machinery, African trypanosomes apparently respond to persistent ER stress by a UPR-like response, including upregulation of BiP, and a related spliced leader silencing (SLS) response whereby SL RNA transcription is shut down. Initially observed by knockdown of the secretory protein translocation machinery, both responses are also induced by chemical agents known to elicit UPR in mammalian cells (H. Read More

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http://dx.doi.org/10.1128/EC.00118-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621318PMC
November 2015
5 Reads

A MORN Repeat Protein Facilitates Protein Entry into the Flagellar Pocket of Trypanosoma brucei.

Eukaryot Cell 2015 Nov 28;14(11):1081-93. Epub 2015 Aug 28.

Department of Cell Biology and Ultrastructure Research, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria

The parasite Trypanosoma brucei lives in the bloodstream of infected mammalian hosts, fully exposed to the adaptive immune system. It relies on a very high rate of endocytosis to clear bound antibodies from its cell surface. All endo- and exocytosis occurs at a single site on its plasma membrane, an intracellular invagination termed the flagellar pocket. Read More

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http://dx.doi.org/10.1128/EC.00094-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621311PMC
November 2015
3 Reads

Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus.

Eukaryot Cell 2015 Nov 28;14(11):1073-80. Epub 2015 Aug 28.

Department of Genetics, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA

Low rates of homologous recombination have broadly encumbered genetic studies in the fungal pathogen Aspergillus fumigatus. The CRISPR/Cas9 system of bacteria has recently been developed for targeted mutagenesis of eukaryotic genomes with high efficiency and, importantly, through a mechanism independent of homologous repair machinery. As this new technology has not been developed for use in A. Read More

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http://dx.doi.org/10.1128/EC.00107-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621320PMC
November 2015
49 Reads

MIG1 Regulates Resistance of Candida albicans against the Fungistatic Effect of Weak Organic Acids.

Eukaryot Cell 2015 Oct 21;14(10):1054-61. Epub 2015 Aug 21.

Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore

Candida albicans is the leading cause of fungal infections; but it is also a member of the human microbiome, an ecosystem of thousands of microbial species potentially influencing the outcome of host-fungal interactions. Accordingly, antibacterial therapy raises the risk of candidiasis, yet the underlying mechanism is currently not fully understood. We hypothesize the existence of bacterial metabolites that normally control C. Read More

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http://dx.doi.org/10.1128/EC.00129-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588629PMC
October 2015
4 Reads

Differences in Host Innate Responses among Coccidioides Isolates in a Murine Model of Pulmonary Coccidioidomycosis.

Eukaryot Cell 2015 Oct 14;14(10):1043-53. Epub 2015 Aug 14.

Northern Arizona Center for Valley Fever Research, Division of Pathogen Genomics, Translational Genomics Research Institute North, Flagstaff, Arizona, USA Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, USA Valley Fever Center for Excellence, University of Arizona, Tucson, Arizona, USA

Coccidioides immitis and Coccidioides posadasii are soil-dwelling fungi and the causative agents of coccidioidomycosis, a mycosis endemic to certain semiarid regions in the Americas. The most common route of infection is by inhalation of airborne Coccidioides arthroconidia. Once a susceptible host inhales the conidia, a transition to mature endosporulated spherules can occur within the first 5 days of infection. Read More

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http://dx.doi.org/10.1128/EC.00122-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588628PMC
October 2015
10 Reads

Eisosome Ultrastructure and Evolution in Fungi, Microalgae, and Lichens.

Eukaryot Cell 2015 Oct 7;14(10):1017-42. Epub 2015 Aug 7.

Department of Biology, Washington University, St. Louis, Missouri, USA

Eisosomes are among the few remaining eukaryotic cellular differentations that lack a defined function(s). These trough-shaped invaginations of the plasma membrane have largely been studied in Saccharomyces cerevisiae, in which their associated proteins, including two BAR domain proteins, have been identified, and homologues have been found throughout the fungal radiation. Using quick-freeze deep-etch electron microscopy to generate high-resolution replicas of membrane fracture faces without the use of chemical fixation, we report that eisosomes are also present in a subset of red and green microalgae as well as in the cysts of the ciliate Euplotes. Read More

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http://dx.doi.org/10.1128/EC.00106-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588622PMC
October 2015
7 Reads

ERG2 and ERG24 Are Required for Normal Vacuolar Physiology as Well as Candida albicans Pathogenicity in a Murine Model of Disseminated but Not Vaginal Candidiasis.

Eukaryot Cell 2015 Oct 31;14(10):1006-16. Epub 2015 Jul 31.

Department of Microbiology, Immunology and Parasitology, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA Department of Clinical Pharmacy, Division of Clinical and Experimental Therapeutics, College of Pharmacy, University of Tennessee Health Sciences Center, Memphis, Tennessee, USA

Several important classes of antifungal agents, including the azoles, act by blocking ergosterol biosynthesis. It was recently reported that the azoles cause massive disruption of the fungal vacuole in the prevalent human pathogen Candida albicans. This is significant because normal vacuolar function is required to support C. Read More

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http://dx.doi.org/10.1128/EC.00116-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588627PMC
October 2015
7 Reads

The Octatricopeptide Repeat Protein Raa8 Is Required for Chloroplast trans Splicing.

Eukaryot Cell 2015 Oct 24;14(10):998-1005. Epub 2015 Jul 24.

Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr-University Bochum, Bochum, Germany

The mRNA maturation of the tripartite chloroplast psaA gene from the green alga Chlamydomonas reinhardtii depends on various nucleus-encoded factors that participate in trans splicing of two group II introns. Recently, a multiprotein complex was identified that is involved in processing the psaA precursor mRNA. Using coupled tandem affinity purification (TAP) and mass spectrometry analyses with the trans-splicing factor Raa4 as a bait protein, we recently identified a multisubunit ribonucleoprotein (RNP) complex comprising the previously characterized trans-splicing factors Raa1, Raa3, Raa4, and Rat2 plus novel components. Read More

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http://dx.doi.org/10.1128/EC.00096-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588620PMC
October 2015
23 Reads

Transcriptome Analysis of Aspergillus flavus Reveals veA-Dependent Regulation of Secondary Metabolite Gene Clusters, Including the Novel Aflavarin Cluster.

Eukaryot Cell 2015 Oct 24;14(10):983-97. Epub 2015 Jul 24.

Department of Biological Sciences, Northern Illinois University, DeKalb, Illinois, USA

The global regulatory veA gene governs development and secondary metabolism in numerous fungal species, including Aspergillus flavus. This is especially relevant since A. flavus infects crops of agricultural importance worldwide, contaminating them with potent mycotoxins. Read More

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http://dx.doi.org/10.1128/EC.00092-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588625PMC
October 2015
5 Reads

Analysis of the Aspergillus fumigatus Biofilm Extracellular Matrix by Solid-State Nuclear Magnetic Resonance Spectroscopy.

Eukaryot Cell 2015 Nov 10;14(11):1064-72. Epub 2015 Jul 10.

Department of Chemistry, Stanford University, Stanford, California, USA

Aspergillus fumigatus is commonly responsible for lethal fungal infections among immunosuppressed individuals. A. fumigatus forms biofilm communities that are of increasing biomedical interest due to the association of biofilms with chronic infections and their increased resistance to antifungal agents and host immune factors. Read More

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http://ec.asm.org/content/14/11/1064.full.pdf
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http://ec.asm.org/content/early/2015/07/08/EC.00050-15.full.
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http://ec.asm.org/lookup/doi/10.1128/EC.00050-15
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http://dx.doi.org/10.1128/EC.00050-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621319PMC
November 2015
14 Reads

Activation of Autophagy by Metals in Chlamydomonas reinhardtii.

Eukaryot Cell 2015 Sep 10;14(9):964-73. Epub 2015 Jul 10.

Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas (CSIC)-Universidad de Sevilla, Seville, Spain

Autophagy is an intracellular self-degradation pathway by which eukaryotic cells recycle their own material in response to specific stress conditions. Exposure to high concentrations of metals causes cell damage, although the effect of metal stress on autophagy has not been explored in photosynthetic organisms. In this study, we investigated the effect of metal excess on autophagy in the model unicellular green alga Chlamydomonas reinhardtii. Read More

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http://dx.doi.org/10.1128/EC.00081-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551596PMC
September 2015
7 Reads

Characterization of Virulence-Related Phenotypes in Candida Species of the CUG Clade.

Eukaryot Cell 2015 Sep 6;14(9):931-40. Epub 2015 Jul 6.

Department of Microbiology and Molecular Genetics and the Graduate School of Biomedical Sciences, University of Texas Health Science Center at Houston, Houston, Texas, USA

Candida species cause a variety of mucosal and invasive infections and are, collectively, the most important human fungal pathogens in the developed world. The majority of these infections result from a few related species within the "CUG clade," so named because they use a nonstandard translation for that codon. Some members of the CUG clade, such as Candida albicans, present significant clinical problems, whereas others, such as Candida (Meyerozyma) guilliermondii, are uncommon in patients. Read More

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http://dx.doi.org/10.1128/EC.00062-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551586PMC
September 2015
5 Reads

A Cyclic GMP-Dependent K+ Channel in the Blastocladiomycete Fungus Blastocladiella emersonii.

Eukaryot Cell 2015 Sep 6;14(9):958-63. Epub 2015 Jul 6.

Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brazil

Phototaxis in flagellated zoospores of the aquatic fungus Blastocladiella emersonii depends on a novel photosensor, Blastocladiella emersonii GC1 (BeGC1), comprising a type I (microbial) rhodopsin fused to a guanylyl cyclase catalytic domain, that produces the conserved second messenger cyclic GMP (cGMP). The rapid and transient increase in cGMP levels during the exposure of zoospores to green light was shown to be necessary for phototaxis and dependent on both rhodopsin function and guanylyl cyclase activity. It is noteworthy that BeGC1 was localized to the zoospore eyespot apparatus, in agreement with its role in the phototactic response. Read More

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http://dx.doi.org/10.1128/EC.00087-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551585PMC
September 2015
15 Reads

Conserved Mode of Interaction between Yeast Bro1 Family V Domains and YP(X)nL Motif-Containing Target Proteins.

Eukaryot Cell 2015 Oct 6;14(10):976-82. Epub 2015 Jul 6.

Laboratory of Protein Metabolism, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.

Yeast Bro1 and Rim20 belong to a family of proteins which possess a common architecture of Bro1 and V domains. Alix and His domain protein tyrosine phosphatase (HD-PTP), mammalian Bro1 family proteins, bind YP(X)nL (n = 1 to 3) motifs in their target proteins through their V domains. In Alix, the Phe residue, which is located in the hydrophobic groove of the V domain, is critical for binding to the YP(X)nL motif. Read More

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http://ec.asm.org/content/14/10/976.full.pdf
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http://ec.asm.org/lookup/doi/10.1128/EC.00091-15
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http://dx.doi.org/10.1128/EC.00091-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588623PMC
October 2015
7 Reads

Ineffective Phosphorylation of Mitogen-Activated Protein Kinase Hog1p in Response to High Osmotic Stress in the Yeast Kluyveromyces lactis.

Eukaryot Cell 2015 Sep 6;14(9):922-30. Epub 2015 Jul 6.

Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México D.F., México

When treated with a hyperosmotic stimulus, Kluyveromyces lactis cells respond by activating the mitogen-activated protein kinase (MAPK) K. lactis Hog1 (KlHog1) protein via two conserved branches, SLN1 and SHO1. Mutants affected in only one branch can cope with external hyperosmolarity by activating KlHog1p by phosphorylation, except for single ΔKlste11 and ΔKlste50 mutants, which showed high sensitivity to osmotic stress, even though the other branch (SLN1) was intact. Read More

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http://ec.asm.org/lookup/doi/10.1128/EC.00048-15
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http://dx.doi.org/10.1128/EC.00048-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551593PMC
September 2015
14 Reads

Interplay between Gliotoxin Resistance, Secretion, and the Methyl/Methionine Cycle in Aspergillus fumigatus.

Eukaryot Cell 2015 Sep 6;14(9):941-57. Epub 2015 Jul 6.

Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland

Mechanistic studies on gliotoxin biosynthesis and self-protection in Aspergillus fumigatus, both of which require the gliotoxin oxidoreductase GliT, have revealed a rich landscape of highly novel biochemistries, yet key aspects of this complex molecular architecture remain obscure. Here we show that an A. fumigatus ΔgliA strain is completely deficient in gliotoxin secretion but still retains the ability to efflux bisdethiobis(methylthio)gliotoxin (BmGT). Read More

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http://dx.doi.org/10.1128/EC.00055-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551589PMC
September 2015
14 Reads

The Centriole Cartwheel Protein SAS-6 in Trypanosoma brucei Is Required for Probasal Body Biogenesis and Flagellum Assembly.

Eukaryot Cell 2015 Sep 26;14(9):898-907. Epub 2015 Jun 26.

Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, Houston, Texas, USA

The centriole in eukaryotes functions as the cell's microtubule-organizing center (MTOC) to nucleate spindle assembly, and its biogenesis requires an evolutionarily conserved protein, SAS-6, which assembles the centriole cartwheel. Trypanosoma brucei, an early branching protozoan, possesses the basal body as its MTOC to nucleate flagellum biogenesis. However, little is known about the components of the basal body and their roles in basal body biogenesis and flagellum assembly. Read More

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http://dx.doi.org/10.1128/EC.00083-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551587PMC
September 2015
4 Reads

Functional Analysis of Sterol Transporter Orthologues in the Filamentous Fungus Aspergillus nidulans.

Eukaryot Cell 2015 Sep 26;14(9):908-21. Epub 2015 Jun 26.

Karlsruhe Institute of Technology, Institute for Applied Biosciences, Department of Microbiology, Karlsruhe, Germany University of Tsukuba, Faculty of Life and Environmental Sciences, Tsukuba, Ibaraki, Japan

Polarized growth in filamentous fungi needs a continuous supply of proteins and lipids to the growing hyphal tip. One of the important membrane compounds in fungi is ergosterol. At the apical plasma membrane ergosterol accumulations, which are called sterol-rich plasma membrane domains (SRDs). Read More

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http://dx.doi.org/10.1128/EC.00027-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551590PMC
September 2015
3 Reads

Auxotrophic Mutations Reduce Tolerance of Saccharomyces cerevisiae to Very High Levels of Ethanol Stress.

Eukaryot Cell 2015 Sep 26;14(9):884-97. Epub 2015 Jun 26.

Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Leuven-Heverlee, Flanders, Belgium Department of Molecular Microbiology, VIB, Leuven-Heverlee, Flanders, Belgium

Very high ethanol tolerance is a distinctive trait of the yeast Saccharomyces cerevisiae with notable ecological and industrial importance. Although many genes have been shown to be required for moderate ethanol tolerance (i.e. Read More

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http://dx.doi.org/10.1128/EC.00053-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551594PMC
September 2015
63 Reads

Comparative Analysis of Transmembrane Regulators of the Filamentous Growth Mitogen-Activated Protein Kinase Pathway Uncovers Functional and Regulatory Differences.

Eukaryot Cell 2015 Sep 26;14(9):868-83. Epub 2015 Jun 26.

Department of Biological Sciences at the University at Buffalo, Buffalo, New York, USA

Filamentous growth is a microbial differentiation response that involves the concerted action of multiple signaling pathways. In budding yeast, one pathway that regulates filamentous growth is a Cdc42p-dependent mitogen-activated protein kinase (MAPK) pathway. Several transmembrane (TM) proteins regulate the filamentous growth pathway, including the signaling mucin Msb2p, the tetraspan osmosensor Sho1p, and an adaptor Opy2p. Read More

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http://dx.doi.org/10.1128/EC.00085-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551592PMC
September 2015
124 Reads
1 Citation

Developmental Coordination of Gamete Differentiation with Programmed Cell Death in Sporulating Yeast.

Eukaryot Cell 2015 Sep 19;14(9):858-67. Epub 2015 Jun 19.

Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada

The gametogenesis program of the budding yeast Saccharomyces cerevisiae, also known as sporulation, employs unusual internal meiotic divisions, after which all four meiotic products differentiate within the parental cell. We showed previously that sporulation is typically accompanied by the destruction of discarded immature meiotic products through their exposure to proteases released from the mother cell vacuole, which undergoes an apparent programmed rupture. Here we demonstrate that vacuolar rupture contributes to de facto programmed cell death (PCD) of the meiotic mother cell itself. Read More

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http://dx.doi.org/10.1128/EC.00068-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551598PMC
September 2015
3 Reads

Acetylcholine Protects against Candida albicans Infection by Inhibiting Biofilm Formation and Promoting Hemocyte Function in a Galleria mellonella Infection Model.

Eukaryot Cell 2015 Aug 19;14(8):834-44. Epub 2015 Jun 19.

Infection and Immunity Research Group, Glasgow Dental School, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom

Both neuronal acetylcholine and nonneuronal acetylcholine have been demonstrated to modulate inflammatory responses. Studies investigating the role of acetylcholine in the pathogenesis of bacterial infections have revealed contradictory findings with regard to disease outcome. At present, the role of acetylcholine in the pathogenesis of fungal infections is unknown. Read More

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http://dx.doi.org/10.1128/EC.00067-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519745PMC
August 2015
8 Reads

Secretion of Polypeptide Crystals from Tetrahymena thermophila Secretory Organelles (Mucocysts) Depends on Processing by a Cysteine Cathepsin, Cth4p.

Eukaryot Cell 2015 Aug 19;14(8):817-33. Epub 2015 Jun 19.

Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois, USA

In many organisms, sophisticated mechanisms facilitate release of peptides in response to extracellular stimuli. In the ciliate Tetrahymena thermophila, efficient peptide secretion depends on specialized vesicles called mucocysts that contain dense crystalline cores that expand rapidly during exocytosis. Core assembly depends of endoproteolytic cleavage of mucocyst proproteins by an aspartyl protease, cathepsin 3 (CTH3). Read More

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http://dx.doi.org/10.1128/EC.00058-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519746PMC
August 2015
8 Reads

A Minimal Set of Glycolytic Genes Reveals Strong Redundancies in Saccharomyces cerevisiae Central Metabolism.

Eukaryot Cell 2015 Aug 12;14(8):804-16. Epub 2015 Jun 12.

Department of Biotechnology, Delft University of Technology, Delft, The Netherlands

As a result of ancestral whole-genome and small-scale duplication events, the genomes of Saccharomyces cerevisiae and many eukaryotes still contain a substantial fraction of duplicated genes. In all investigated organisms, metabolic pathways, and more particularly glycolysis, are specifically enriched for functionally redundant paralogs. In ancestors of the Saccharomyces lineage, the duplication of glycolytic genes is purported to have played an important role leading to S. Read More

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http://dx.doi.org/10.1128/EC.00064-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519752PMC
August 2015
13 Reads

The N-Linked Outer Chain Mannans and the Dfg5p and Dcw1p Endo-α-1,6-Mannanases Are Needed for Incorporation of Candida albicans Glycoproteins into the Cell Wall.

Eukaryot Cell 2015 Aug 5;14(8):792-803. Epub 2015 Jun 5.

Department of Biological Sciences, SUNY University at Buffalo, Buffalo, New York, USA

A biochemical pathway for the incorporation of cell wall protein into the cell wall of Neurospora crassa was recently proposed. In this pathway, the DFG-5 and DCW-1 endo-α-1,6-mannanases function to covalently cross-link cell wall protein-associated N-linked galactomannans, which are structurally related to the yeast outer chain mannans, into the cell wall glucan-chitin matrix. In this report, we demonstrate that the mannosyltransferase enzyme Och1p, which is needed for the synthesis of the N-linked outer chain mannan, is essential for the incorporation of cell wall glycoproteins into the Candida albicans cell wall. Read More

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http://ec.asm.org/content/early/2015/06/01/EC.00032-15.full.
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http://ec.asm.org/content/14/8/792.full.pdf
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http://ec.asm.org/lookup/doi/10.1128/EC.00032-15
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http://dx.doi.org/10.1128/EC.00032-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519743PMC
August 2015
15 Reads

Evidence for Extracellular ATP as a Stress Signal in a Single-Celled Organism.

Eukaryot Cell 2015 Aug 5;14(8):775-82. Epub 2015 Jun 5.

School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, United Kingdom

ATP is omnipresent in biology and acts as an extracellular signaling molecule in mammals. Information regarding the signaling function of extracellular ATP in single-celled eukaryotes is lacking. Here, we explore the role of extracellular ATP in cell volume recovery during osmotic swelling in the amoeba Dictyostelium. Read More

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http://dx.doi.org/10.1128/EC.00066-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519744PMC
August 2015
3 Reads

Deletion of the DNA Ligase IV Gene in Candida glabrata Significantly Increases Gene-Targeting Efficiency.

Eukaryot Cell 2015 Aug 5;14(8):783-91. Epub 2015 Jun 5.

Department of Molecular Microbiology, VIB, KU Leuven, Leuven, Belgium Laboratory of Molecular Cell Biology, KU Leuven, Leuven, Belgium

Candida glabrata is reported as the second most prevalent human opportunistic fungal pathogen in the United States. Over the last decades, its incidence increased, whereas that of Candida albicans decreased slightly. One of the main reasons for this shift is attributed to the inherent tolerance of C. Read More

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http://dx.doi.org/10.1128/EC.00281-14DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519754PMC
August 2015
5 Reads

Lipid Biosynthetic Genes Affect Candida albicans Extracellular Vesicle Morphology, Cargo, and Immunostimulatory Properties.

Eukaryot Cell 2015 Aug 29;14(8):745-54. Epub 2015 May 29.

Department of Microbiology, Johns Hopkins School of Public Health, Baltimore, Maryland, USA

Microbial secretion is integral for regulating cell homeostasis as well as releasing virulence factors during infection. The genes encoding phosphatidylserine synthase (CHO1) and phosphatidylserine decarboxylase (PSD1 and PSD2) are Candida albicans genes involved in phospholipid biosynthesis, and mutations in these genes affect mitochondrial function, cell wall thickness, and virulence in mice. We tested the roles of these genes in several agar-based secretion assays and observed that the cho1Δ/Δ and psd1Δ/Δ psd2Δ/Δ strains manifested less protease and phospholipase activity. Read More

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http://dx.doi.org/10.1128/EC.00054-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519749PMC
August 2015
8 Reads

Candida albicans Kinesin Kar3 Depends on a Cik1-Like Regulatory Partner Protein for Its Roles in Mating, Cell Morphogenesis, and Bipolar Spindle Formation.

Eukaryot Cell 2015 Aug 29;14(8):755-74. Epub 2015 May 29.

Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada

Candida albicans is a major fungal pathogen whose virulence is associated with its ability to transition from a budding yeast form to invasive hyphal filaments. The kinesin-14 family member CaKar3 is required for transition between these morphological states, as well as for mitotic progression and karyogamy. While kinesin-14 proteins are ubiquitous, CaKar3 homologs in hemiascomycete fungi are unique because they form heterodimers with noncatalytic kinesin-like proteins. Read More

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http://dx.doi.org/10.1128/EC.00015-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519750PMC
August 2015
9 Reads

Rgc2 Regulator of Glycerol Channel Fps1 Functions as a Homo- and Heterodimer with Rgc1.

Eukaryot Cell 2015 Jul 29;14(7):719-25. Epub 2015 May 29.

Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts, USA Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts, USA

The plasma membrane aquaglyceroporin Fps1 is responsible for glycerol transport in yeast in response to changes in extracellular osmolarity. Fps1 functions as a homotetramer, and control of its channel activity in response to hyperosmotic shock involves a redundant pair of fungus-specific regulators, Rgc1 and Rgc2 (regulators of the glycerol channel), and the mitogen-activatd protein kinase (MAPK) Hog1 (high-osmolarity glycerol response). Rgc1 and Rgc2 maintain Fps1 in an open-channel state by binding to its C-terminal cytoplasmic domain. Read More

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http://dx.doi.org/10.1128/EC.00073-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4486678PMC
July 2015
6 Reads