63,291 results match your criteria Escherichia Coli Infections

Pogostone Enhances the Antibacterial Activity of Colistin against MCR-1-Positive Bacteria by Inhibiting the Biological Function of MCR-1.

Molecules 2022 Apr 28;27(9). Epub 2022 Apr 28.

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.

The emergence of the plasmid-mediated colistin resistance gene has resulted in the loss of available treatments for certain severe infections. Here we identified a potential inhibitor of MCR-1 for the treatment of infections caused by MCR-1-positive drug-resistant bacteria, especially MCR-1-positive carbapenem-resistant (CRE). A checkerboard minimum inhibitory concentration (MIC) test, a killing curve test, a growth curve test, bacterial live/dead assays, scanning electron microscope (SEM) analysis, cytotoxicity tests, molecular dynamics simulation analysis, and animal studies were used to confirm the in vivo/in vitro synergistic effects of pogostone and colistin. Read More

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Molecular and Serological Characteristics of Avian Pathogenic Isolated from Various Clinical Cases of Poultry Colibacillosis in Poland.

Animals (Basel) 2022 Apr 22;12(9). Epub 2022 Apr 22.

Department of Veterinary Prevention and Avian Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland.

infections are a major problem in modern poultry production. Avian pathogenic (APEC) strains have several mechanisms that enable them to colonize various ecosystems. In this study, 290 isolates were recovered from clinical cases of colibacillosis in chicken and turkey broilers and from laying and breeding hens. Read More

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Molecular Epidemiology of Antibiotic Resistance Genes and Virulence Factors in Multidrug-Resistant Isolated from Rodents, Humans, Chicken, and Household Soils in Karatu, Northern Tanzania.

Int J Environ Res Public Health 2022 Apr 28;19(9). Epub 2022 Apr 28.

SACIDS Foundation for One Health, Sokoine University of Agriculture, P.O. Box 3297, Morogoro 67125, Tanzania.

The interaction of rodents with humans and chicken in the household environment can facilitate transmission of multidrug-resistant (MDR) (), causing infections that are difficult to treat. We investigated the presence of genes encoded for carbapenem, extended spectrum beta-lactamases (ESBL), tetracycline and quinolones resistance, and virulence among 50 MDR isolated from human ( = 14), chicken ( = 12), rodent ( = 10), and soil ( = 14) samples using multiplex polymerase chain reaction (PCR). Overall, the antimicrobial resistance genes (ARGs) detected were: 23/50 (46%), 13/50 (26%), 23/50 (46%), 7/50 (14%), 12/50 (24%), 4/50 (8%), 6/50 (12%), and 3/50 (6%), while , , and were not found. Read More

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RNase 7 Inhibits Uropathogenic -Induced Inflammation in Bladder Cells under a High-Glucose Environment by Regulating the JAK/STAT Signaling Pathway.

Int J Mol Sci 2022 May 5;23(9). Epub 2022 May 5.

Department of Molecular Parasitology and Tropical Diseases, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.

Antimicrobial peptides (AMPs), which are natural antibiotics, protect against pathogens invading the urinary tract. RNase 7 with antimicrobial properties has rapid and powerful suppressive effects against Gram-positive and Gram-negative bacterial infections. However, its detailed antibacterial mechanisms have not been fully determined. Read More

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Impact of Bacterial Infections and SBP Prophylaxis on Phage-Bacterial Dynamics in Cirrhosis.

Hepatology 2022 May 13. Epub 2022 May 13.

George Mason University, Manassas, Virginia, USA.

Gut microbiota, including bacteria and phages are altered in cirrhosis, but their role during infections and SBP prophylaxis is unclear. Aim: Determine metagenomic changes in gut bacteria, phages and their linkages centered around gram-negative and positive pathobionts in patients with cirrhosis with/without infections or SBP prophylaxis. We included uninfected (n=231) and infected (n=30, SBP n=19 and UTI n=11, before antibiotics) patients who gave stool for bacterial and phage metagenomics. Read More

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ARGs Prevalence Among Hospitalized COVID-19 Patients with Bacterial Infections and Resistome abundance.

Mahmoud Aly

FASEB J 2022 May;36 Suppl 1

Biological Studies, York College of Pennsylvania, York, PA.

Background: The burden of antimicrobial resistance AMR worldwide is substantial and is growing. Many factors play a role in the emergence of resistance. Our microbiome is a significant reservoir for antimicrobial resistance genes (ARGs). Read More

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Optimizing the production, purification, characterization of E. coli OMVs.

FASEB J 2022 May;36 Suppl 1

Rochester Institute of Technology, Rochester, NY.

Outer membrane vesicles (OMVs) are 20-250 nm particles released from Gram-negative bacteria, including commensal and pathogenic Escherichia coli(E. coli). OMVs contain outer membrane and periplasmic proteins, toxins, nucleic acids, and pieces of peptidoglycan. Read More

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Treatment of Multidrug-Resistant Bacterial Infections Using Quantum Dots.

FASEB J 2022 May;36 Suppl 1

University of Colorado Boulder, Boulder, CO.

Multidrug-resistant (MDR) bacterial infections remain a major threat to public health, despite strides made in antibiotic development in the past century. The rise of MDR infections has demonstrated the dire need for new treatment strategies against infectious disease. MDR bacteria are predicted to cause ten million deaths annually worldwide by 2050, more than all cancers combined, unless significant strides are made in treatment options. Read More

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Resistance profile of bacteria to antibiotics in city laboratories.

FASEB J 2022 May;36 Suppl 1

HMIMV, Rabat, Rabat.

Introduction: Bacteria are the causative agents most frequently described in infections. Therefore, antibiotic resistance is a real public health problem. Thus, bacterial infections are becoming one of the major threats to public health in the world. Read More

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Inhibition of Escherichia coliATP synthase by dietary pomegranate phenolics.

FASEB J 2022 May;36 Suppl 1

Biochemistry, A.T. Still University, Kirksville, MO.

Background: A wide range of natural and synthetic compounds bind and inhibit ATP synthase. Dietary polyphenols found in fruits, vegetables, and spices such as tea, olives, grapes, cantaloupes, garlic, and pomegranate possess antimicrobial activities. Dietary pomegranate polyphenols (DPPs) have been shown to exhibit anticancer, antioxidant, and antimicrobial properties. Read More

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Metabolic Regulation of Quiescence and Antibiotic Tolerance in Uropathogenic Escherichia coli.

FASEB J 2022 May;36 Suppl 1

Cell and Molecular Biology, University of Rhode Island, Kingston, RI.

Urinary tract infections (UTIs) pose a global public health threat. Uropathogenic Escherichia coli (UPEC) cause the majority of UTIs and a quarter of infections are recurrent within six months. This may be attributed to UPEC entering a non-proliferative, or quiescent state that allows some cell populations to evade the host immune response and survive exposure to antibiotics, thus causing reinfection upon cessation of antibiotic treatment. Read More

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Manganese Homeostasis in Bacteria: Interaction of the Small Protein MntS and Manganese Exporter MntP in E. coli.

FASEB J 2022 May;36 Suppl 1

University of Wisconsin Oshkosh, Oshkosh, WI.

Manganese (Mn) serves as a vital cofactor for bacterial survival, protecting bacteria from reactive oxygen species, radiation, and acidic environments. Bacterial metal ion regulation is associated with pathogenicity, as many virulent strains of bacteria upregulate their ability to obtain metal ions from host species. However, excess metal ions quickly become toxic for most bacteria, resulting in tightly regulated cytoplasmic metal ion concentrations. Read More

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CTX-M-producing strains: resistance to temocillin, fosfomycin, nitrofurantoin and biofilm formation.

Future Microbiol 2022 May 13. Epub 2022 May 13.

Institute of Cardiology, Lithuanian University of Health Sciences, Kaunas, LT-50161, Lithuania.

ESBL-producing and bacterial biofilms-forming are associated with antimicrobial treatment failure. This study aimed to investigate the phenotypic resistance mechanisms of CTX-M against old antibiotics - cell wall synthesis inhibitors temocillin, nitrofurantoin and fosfomycin. Susceptibility to old antibiotics testing was performed using disk diffusion method, biofilm formation was evaluated spectrophotometrically, and PCR was used for the determination of CTX-M type. Read More

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Main-Chain Sulfonium-Containing Homopolymers with Negligible Hemolytic Toxicity for Eradication of Bacterial and Fungal Biofilms.

ACS Macro Lett 2021 Dec 10;10(12):1643-1649. Epub 2021 Dec 10.

Hubei Key Laboratory of Material Chemistry and Service Failure, Hubei Engineering Research Center for Biomaterials and Medical Protective Materials, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, Hubei 430074, People's Republic of China.

Antimicrobials against planktonic cells and established biofilms at low doses are in increasing demand to tackle antibiotic-resistant biofilm infections. As a promising alternative to antibiotics, cationic polymers can effectively kill planktonic microbes but usually require high concentrations to eradicate the established biofilms. Herein, we developed a series of sulfonium-based homopolymers with cationic sulfoniums and alkane spacers in the main chain. Read More

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December 2021

Bacteriophage Cocktail and Microcin-Producing Probiotic Protect Mice Against Gut Colonization With Multidrug-Resistant Sequence Type 131.

Front Microbiol 2022 25;13:887799. Epub 2022 Apr 25.

Minneapolis VA Health Care System, Veterans Health Administration, United States Department of Veterans Affairs, Minneapolis, MN, United States.

Non-antibiotic measures are needed to reduce the rate of infections due to multidrug-resistant organisms (MDROs), including by eliminating the commensal reservoir that underlies such strains' dissemination and leads to recurrent infections. Here, we tested a cocktail of pre-selected bacteriophages and an engineered microcin C7-producing probiotic Nissle-1917 strain for their ability to reduce gut colonization by an strain from sequence type 131 (ST131)-30R, which is the major clonal group of MDROs among extraintestinal clinical isolates. Although the bacteriophage cocktail was highly effective against ST131-30R strains both and in a murine model of subcutaneous sepsis, it was only weakly and transiently effective against gut colonization by the target ST131-30R strain (0. Read More

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Study of Bacterial Contamination in Operating Theatres at Al-Hussein Teaching Hospital in Al-Samawah, Iraq.

Arch Razi Inst 2021 Dec 30;76(6):1671-1676. Epub 2021 Dec 30.

Al-Muthanna Governorate Health Department, Samawah, Iraq.

The continuing bacterial contamination in hospitals operating units and theaters has an important role in the spread of hospital infections. The current study evaluated the level of bacterial contamination in the operating theatres at Al-Hussein Teaching Hospital in Al-Samawah, Iraq. For the purpose of sample collection from surgical equipment surfaces, the swab plate method conducted by nurses and laboratory workers was used to collect the samples in the operating theater. Read More

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December 2021

Urachal Actinomycosis Presenting with Recurrent Urinary Tract Infections in a Middle-aged Woman: A Rare Case Report.

J Coll Physicians Surg Pak 2022 May;32(5):668-670

Department of Pathology, Hisar Intercontinental Hospital, Istanbul, Turkey.

The urachal cyst, a remnant of allantois sac during embryogenesis, is a rare condition in adulthood. Urachus is an embryologic remnant degenerating after birth. Abberrant obliteration of the urachus causes urachal abnormalities. Read More

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Persisting uropathogenic Escherichia coli lineages show signatures of niche-specific within-host adaptation mediated by mobile genetic elements.

Cell Host Microbe 2022 May 9. Epub 2022 May 9.

The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA; Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA. Electronic address:

Large-scale genomic studies have identified within-host adaptation as a hallmark of bacterial infections. However, the impact of physiological, metabolic, and immunological differences between distinct niches on the pathoadaptation of opportunistic pathogens remains elusive. Here, we profile the within-host adaptation and evolutionary trajectories of 976 isolates representing 119 lineages of uropathogenic Escherichia coli (UPEC) sampled longitudinally from both the gastrointestinal and urinary tracts of 123 patients with urinary tract infections. Read More

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Expression analysis of TLR signaling pathway genes under lipopolysaccharide-induced and F17-infected sheep intestinal epithelial cells.

Anim Biotechnol 2022 May 11:1-7. Epub 2022 May 11.

College of Animal Science and Technology, Yangzhou University, Yangzhou, PR China.

() F17 is one of the main pathogens causing diarrhea in young livestock. The specific F17 fimbriae and lipopolysaccharide (LPS) in the surface components of F17 induces immune activation interacting with the intestinal epithelial cells (IECs)-expressed innate immune toll-like receptors (TLRs) signaling pathway. In this study, the expression patterns of eight canonical genes from the TLR signaling pathway (, , , , , , and ) were analyzed in LPS-induced IECs, F17-infected IECs and ileum tissue of F17-infected lambs. Read More

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Antimicrobial resistance genes and clonal success in Escherichia coli isolates causing bloodstream infection.

Lancet Microbe 2021 Oct 12;2(10):e492. Epub 2021 Aug 12.

Nuffield Department of Medicine, University of Oxford, Oxford OX3 9DU, UK; Oxford University Hospitals NHS Foundation Trust, Oxford, UK.

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October 2021

Emergence and dissemination of antimicrobial resistance in Escherichia coli causing bloodstream infections in Norway in 2002-17: a nationwide, longitudinal, microbial population genomic study.

Lancet Microbe 2021 Jul 10;2(7):e331-e341. Epub 2021 May 10.

Department of Biostatistics, University of Oslo, Oslo, Norway; Parasites and Microbes, Wellcome Sanger Institute, Cambridge, UK.

Background: The clonal diversity underpinning trends in multidrug resistant Escherichia coli causing bloodstream infections remains uncertain. We aimed to determine the contribution of individual clones to resistance over time, using large-scale genomics-based molecular epidemiology.

Methods: This was a longitudinal, E coli population, genomic, cohort study that sampled isolates from 22 512 E coli bloodstream infections included in the Norwegian surveillance programme on resistant microbes (NORM) from 2002 to 2017. Read More

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Profiling the plasmid conjugation potential of urinary .

Microb Genom 2022 May;8(5)

Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL 60153, USA.

is often associated with urinary tract infection (UTI). Antibiotic resistance in is an ongoing challenge in managing UTI. Extrachromosomal elements - plasmids - are vectors for clinically relevant traits, such as antibiotic resistance, with conjugation being one of the main methods for horizontal propagation of plasmids in bacterial populations. Read More

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Characterization and Utilization of Methyltransferase for Apramycin Production in Streptoalloteichus tenebrarius.

J Ind Microbiol Biotechnol 2022 May 10. Epub 2022 May 10.

School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, People's Republic of China.

A structurally unique aminoglycoside produced in Streptoalloteichus tenebrarius, Apramycin is used in veterinary medicine or the treatment of Salmonella, Escherichia coli and Pasteurella multocida infections. Although apramycin was discovered nearly 50 years ago, many biosynthetic steps of apramycin remain unknown. In this study, we identified a HemK family methyltransferase, AprI, to be the 7'-N-methyltransferase in apramycin biosynthetic pathway. Read More

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Phage Cocktail Targeting STEC O157:H7 Has Comparable Efficacy and Superior Recovery Compared with Enrofloxacin in an Enteric Murine Model.

Microbiol Spectr 2022 May 10:e0023222. Epub 2022 May 10.

MOE Joint International Research Laboratory of Animal Health and Food Safety; Key Laboratory of Animal Bacteriology, Ministry of Agriculture; and College of Veterinary Medicine, Nanjing Agricultural Universitygrid.27871.3b, Nanjing, China.

O157:H7 is the most important Shiga toxin-producing Escherichia coli (STEC) serotype in relation to public health. Given that antibiotics may contribute to the exacerbation of STEC-related disease and an increased frequency of antibiotic-resistant strains, bacteriophage (phage) therapy is considered a promising alternative. However, phage therapy targeting enteric pathogens is still underdeveloped with many confounding effects from the microbiota. Read More

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Microbiome Profiling of Enterotoxigenic Escherichia coli (ETEC) Carriers Highlights Signature Differences between Symptomatic and Asymptomatic Individuals.

mBio 2022 May 10:e0015722. Epub 2022 May 10.

Cambridge Institute for Therapeutic Immunology and Infectious Disease (CITIID), Department of Medicine, University of Cambridge, Cambridge, United Kingdom.

Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrhea in children in low- and middle-income countries (LMICs). However, large-scale pathogen burden studies in children have identified ETEC in the guts of both symptomatic patients and controls. The factors that influence this balance are poorly understood, but it is postulated that the gut microbiome may play a role in either resistance or progression to disease. Read More

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Methenamine hippurate compared with antibiotic prophylaxis to prevent recurrent urinary tract infections in women: the ALTAR non-inferiority RCT.

Health Technol Assess 2022 May;26(23):1-172

Urology Department, Pinderfields Hospital, Wakefield, UK.

Background: Daily, low-dose antibiotic prophylaxis is the current standard care for women with recurrent urinary tract infection. Emerging antimicrobial resistance is a global health concern, prompting research interest in non-antibiotic agents such as methenamine hippurate, but comparative data on their efficacy and safety are lacking.

Objective: To assess the clinical effectiveness and cost-effectiveness of methenamine hippurate (Hiprex; Mylan NV, Canonsburg, PA, USA) compared with current standard care (antibiotic prophylaxis) for recurrent urinary tract infection prevention in adult women. Read More

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Modulating the evolutionary trajectory of tolerance using antibiotics with different metabolic dependencies.

Nat Commun 2022 May 9;13(1):2525. Epub 2022 May 9.

Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.

Antibiotic tolerance, or the ability of bacteria to survive antibiotic treatment in the absence of genetic resistance, has been linked to chronic and recurrent infections. Tolerant cells are often characterized by a low metabolic state, against which most clinically used antibiotics are ineffective. Here, we show that tolerance readily evolves against antibiotics that are strongly dependent on bacterial metabolism, but does not arise against antibiotics whose efficacy is only minimally affected by metabolic state. Read More

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High prevalence of small intestine bacteria overgrowth and asymptomatic carriage of enteric pathogens in stunted children in Antananarivo, Madagascar.

PLoS Negl Trop Dis 2022 May 9;16(5):e0009849. Epub 2022 May 9.

Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, France.

Environmental Enteric Dysfunction (EED) refers to an incompletely defined syndrome of inflammation, reduced absorptive capacity, and reduced barrier function in the small intestine. It is widespread among children and adults in low- and middle-income countries and is also associated with poor sanitation and certain gut infections possibly resulting in an abnormal gut microbiota, small intestinal bacterial overgrowth (SIBO) and stunting. We investigated bacterial pathogen exposure in stunted and non-stunted children in Antananarivo, Madagascar by collecting fecal samples from 464 children (96 severely stunted, 104 moderately stunted and 264 non-stunted) and the prevalence of SIBO in 109 duodenal aspirates from stunted children (61 from severely stunted and 48 from moderately stunted children). Read More

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Anti-biofilm potential of Y3 culture and its cell-free supernatant against multidrug-resistant uropathogen U12.

Saudi J Biol Sci 2022 Apr 15;29(4):2989-2997. Epub 2022 Jan 15.

Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

Uropathogens develop biofilms on urinary catheters, resulting in persistent and chronic infections that are associated with resistance to antimicrobial therapy. Therefore, the current study was performed to control biofilm-associated urinary tract infections through assaying the anti-biofilm ability of lactic acid bacteria (LAB) against multidrug-resistant (MDR) uropathogens. Twenty LAB were obtained from pickles and fermented dairy products, and screened for their anti-biofilm and antimicrobial effects against MDR U12 (ECU12). Read More

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Prevalence, antimicrobial resistance profile, and characterization of multi-drug resistant bacteria from various infected wounds in North Egypt.

Saudi J Biol Sci 2022 Apr 15;29(4):2978-2988. Epub 2022 Jan 15.

Polymer Materials Research Department, Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, P.O. Box: 21934, Alexandria, Egypt.

Multi-drug resistant (MDR) bacteria associated with wounds are extremely escalating. This study aims to survey different wounds in Alexandria hospitals, North Egypt, to explore the prevalence and characteristics of MDR bacteria for future utilization in antibacterial wound dressing designs. Among various bacterial isolates, we determined 22 MDR bacteria could resist different classes of antibiotics. Read More

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