51,410 results match your criteria Escherichia Coli Infections


Genetic analysis of invasive Escherichia coli in Scotland reveals determinants of healthcare-associated versus community-acquired infections.

Microb Genom 2018 Jun 22. Epub 2018 Jun 22.

1​Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, UK.

Bacteraemia caused by Escherichia coli is a growing problem with a significant mortality. The factors that influence the acquisition and outcome of these infections are not clear. Here, we have linked detailed genetic data from the whole-genome sequencing of 162 bacteraemic isolates collected in Scotland, UK, in 2013-2015, with clinical data in order to delineate bacterial and host factors that influence the acquisition in hospital or the community, outcome and antibiotic resistance. Read More

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Clinical and microbiological characteristics of peritoneal dialysis-related peritonitis caused by Escherichia coli in southern Taiwan.

Eur J Clin Microbiol Infect Dis 2018 Jun 21. Epub 2018 Jun 21.

Department of Biotechnology and Laboratory Science in Medicine, School of Biomedical Science and Engineering, National Yang Ming University, No. 155, Section 2, Li-Nong Street, Taipei, 11221, Taiwan.

Peritonitis is a serious complication and major cause of treatment failure in patients undergoing peritoneal dialysis (PD). Escherichia coli is the major pathogen in extraintestinal Gram-negative infections, including PD-related peritonitis. The outcomes of E. Read More

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Antibacterial Activity of Thai Medicinal Plant Extracts Against Microorganism Isolated from Post-Weaning Diarrhea in Piglets.

J Med Assoc Thai 2016 Jul;99 Suppl 4:S203-10

Background: Post-weaning bacterial infections in piglets caused by resistant strains have increased dramatically. Thai medicinal plants such as Piper retrofractum, Piper leptostachyum, Piper sarmentosum, Zingiber officinale, Plumbago indica, Piper betle, Caesalpinia sappan, Garcinia mangostana etc have been widely used in Thai traditional medicine to treat diarrhea. Thus, these plants were investigated antimicrobial activity against gram negative strain isolated from piglets with diarrhea. Read More

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The Role of Ion Transporters in the Pathophysiology of Infectious Diarrhea.

Cell Mol Gastroenterol Hepatol 2018 5;6(1):33-45. Epub 2018 Mar 5.

Department of Medicine, University of California San Diego School of Medicine, La Jolla, California.

Every year, enteric infections and associated diarrhea kill millions of people. The situation is compounded by increases in the number of enteric pathogens that are acquiring resistance to antibiotics, as well as (hitherto) a relative paucity of information on host molecular targets that may contribute to diarrhea. Many forms of diarrheal disease depend on the dysregulation of intestinal ion transporters, and an associated imbalance between secretory and absorptive functions of the intestinal epithelium. Read More

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High molecular weight hyaluronic acid: a two-pronged protectant against infection of the urogenital tract?

Clin Transl Immunology 2018 7;7(6):e1021. Epub 2018 Jun 7.

Institute of Cell and Molecular Biosciences Medical School Newcastle University Newcastle upon Tyne UK.

Objectives: Recurrent urinary tract infections are associated with uropathogenic (UPEC) ascending and infecting the urinary tract. Antibiotics provide only symptomatic relief, not prevent recurrence. Clinical evidence suggests that intravesical glycosaminoglycan therapy, such as hyaluronic acid (HA), helps reduce UTI recurrence. Read More

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Synergy between conventional antibiotics and anti-biofilm peptides in a murine, sub-cutaneous abscess model caused by recalcitrant ESKAPE pathogens.

PLoS Pathog 2018 Jun 21;14(6):e1007084. Epub 2018 Jun 21.

Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.

With the antibiotic development pipeline running dry, many fear that we might soon run out of treatment options. High-density infections are particularly difficult to treat due to their adaptive multidrug-resistance and currently there are no therapies that adequately address this important issue. Here, a large-scale in vivo study was performed to enhance the activity of antibiotics to treat high-density infections caused by multidrug-resistant Gram-positive and Gram-negative bacteria. Read More

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Contribution of Healthy Chickens to Antimicrobial-Resistant Escherichia coli Associated with Human Extraintestinal Infections in Egypt.

Vector Borne Zoonotic Dis 2018 Jun 21. Epub 2018 Jun 21.

2 Bacterial Epidemiology and Antimicrobial Resistance Research Unit, U.S. National Poultry Research Center , USDA-ARS, Athens, Georgia .

Background: Chickens are considered potential reservoirs for human extraintestinal infections with pathogenic Escherichia coli. However, information about genetic relatedness between E. coli from healthy chickens and human patients is still limited. Read More

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Collagen VI Contains Multiple Host Defense Peptides with Potent In Vivo Activity.

J Immunol 2018 Jun 20. Epub 2018 Jun 20.

Division of Infection Medicine, Department of Clinical Sciences, Lund University, 221 84 Lund, Sweden.

Collagen VI is a ubiquitous extracellular matrix component that forms extensive microfibrillar networks in most connective tissues. In this study, we describe for the first time, to our knowledge, that the collagen VI von Willebrand factor type A-like domains exhibit a broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria in human skin infections in vivo. In silico sequence and structural analysis of VWA domains revealed that they contain cationic and amphipathic peptide sequence motifs, which might explain the antimicrobial nature of collagen VI. Read More

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Bacterial RecA Protein Promotes Adenoviral Recombination during Infection.

mSphere 2018 Jun 20;3(3). Epub 2018 Jun 20.

Department of Ophthalmology, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, USA

Adenovirus infections in humans are common and sometimes lethal. Adenovirus-derived vectors are also commonly chosen for gene therapy in human clinical trials. We have shown in previous work that homologous recombination between adenoviral genomes of human adenovirus species D (HAdV-D), the largest and fastest growing HAdV species, is responsible for the rapid evolution of this species. Read More

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June 2018
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Febrile Neutropenia following Parvovirus B19 Infection and Cross Anti-Kell Reaction to E. Coli in Pregnancy.

Rev Bras Ginecol Obstet 2018 Jun 20. Epub 2018 Jun 20.

Department of Obstetrics and Gynecology, Centro Hospitalar Tâmega e Sousa, Porto, Portugal.

Parvovirus B19 has tropism for red line blood cells, causing immune hydrops during pregnancy. A positive anti-Kell Coombs reaction usually happens during pregnancy when there is production of antibodies that target Kell antigens, but cross reactions to other antigens may occur. A 24-year-old Gypsy primigravida, 0 Rhesus positive, presented with persistent isolated hyperthermia for 2 weeks and a positive indirect Coombs test result with anti-Kell antibodies at routine tests. Read More

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[Sensitivity to Fosfomycin in Extended-spectrum Betalactamase-producing Escherichia coli].

Rev Peru Med Exp Salud Publica 2018 Jan-Mar;35(1):68-71

Hospital Nacional Hipólito Unanue. Lima, Perú.

Urinary infections are caused mainly by Escherichia coli (E. coli); indiscriminate use of antibiotics has caused an increase in infections due to extended-spectrum beta-lactamase (ESBL)-producing strains. Aiming to determine the sensitivity to fosfomycin, a study was conducted in ESBL-producing E. Read More

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January 2018

Direct disk testing versus isolation and antimicrobial susceptibility testing of urine from urinary tract infection.

Iran J Microbiol 2018 Feb;10(1):37-44

Department of Clinical Biochemistry, College of Pharmacy, University of Sulaimani, Sulaymaniyah, Iraq.

Background And Objectives: Urinary tract infections are common infections that can be caused by many bacterial pathogens. The susceptibility of such pathogens to antimicrobial agents is identified by different methods including disk diffusion test, direct sensitivity testing and determination minimum inhibitory concentration. The present study was conducted to isolate and identify bacteria cultured from urine samples and compare the results of direct sensitivity test (DST) against Kirby-Bauer's disk diffusion antimicrobial sensitivity (AST) with respect to reliability, time and cost. Read More

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February 2018

Analysis of consumer food purchase data used for outbreak investigations, a review.

Euro Surveill 2018 Jun;23(24)

Department of Infectious Disease Epidemiology and Prevention, Statens Serum Institut, Copenhagen, Denmark.

BackgroundInvestigations of food-borne outbreaks are frequently unsuccessful and new investigation methods should be welcomed. Describe the use of consumer purchase datasets in outbreak investigations and consider methodological and practical difficulties. We reviewed published papers describing the use of consumer purchase datasets, where electronic data on the foods that case-patients had purchased before onset of symptoms were obtained and analysed as part of outbreak investigations. Read More

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[The profile of antibiotic resistantpathogens isolated from ascites fluid patients in intensive care unit during past 12 years.

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2016 Mar;28(3):211-6

Objective: To investigate the profile and antibiotic resistance of bacteria in patients with ascites infection in intensive care unit (ICU) patients in order to provide a reference for rational clinical use of antibiotics.

Methods: A retrospective analysis was conducted. The bacteria isolated from ascetic fluid patients admitted from January 1st, 2004 to October 31st, 2015 to ICU of the First Affiliated Hospital of Anhui Medical University were identified, and their susceptibility to antibiotics was analyzed. Read More

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A rare case of peritonitis due to .

J Community Hosp Intern Med Perspect 2018 12;8(3):161-162. Epub 2018 Jun 12.

Department of Medicine, Internal Medicine Residency Program, Greater Baltimore Medical Center, Baltimore, MD, USA.

Healthcare associated infections due to gram negative bacilli are a major concern among healthcare institutions. While and species are known major organisms; other lesser known species can also cause primary infection. We present a case of peritonitis in a patient on a peritoneal dialysis regimen due to Read More

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June 2018
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Treatment Options for Carbapenem- Resistant Gram-Negative Infections.

Dtsch Arztebl Int 2018 May;115(20-21):345-352

German Center for Infection Research (DZIF); Institute for Medical Microbiology, University of Gießen; Department of Urology, Pediatric Urology, and Andrology, University of Gießen; Clinical Infectiology, Department of Medicine II, University of Gießen; German Center for Lung Research (DZL); Department of General Pediatrics and Neonatology, Center for Pediatric and Adolescent Medicine, University of Gießen.

Background: Rates of colonization and infection with carbapenem-resistant Gram-negative pathogens are on the rise, particularly in southeastern European countries, and this is increasingly true in Germany as well. The organisms in question include enterobacteriaceae such as Klebsiella pneumoniae and Escherichia coli and non-fermenting bacteria such as Pseudomonas aeruginosa and Acinetobacter baumannii. As the carbapenems have been the gold standard to date for the systemic treatment of serious infections with Gram-negative bacteria, carbapenem resistance presents new and difficult challenges in therapeutic decision-making, particularly because of the high frequency of coresistance. Read More

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Importance of case age in the purported association between phylogenetics and hemolytic uremic syndrome in Escherichia coli O157:H7 infections.

Epidemiol Infect 2018 Jun 19:1-6. Epub 2018 Jun 19.

Department of Epidemiology,University of Washington,Seattle,Washington,USA.

Escherichia coli O157:H7 is the largest cause of hemolytic uremic syndrome (HUS). Previous studies proposed that HUS risk varies across the E. coli O157:H7 phylogenetic tree (hypervirulent clade 8), but the role of age in the association is unknown. Read More

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Phylotyping based virulence associated genes and drug susceptibility of Uropathogenic in urinary tract infection patients.

J Microbiol Biotechnol 2018 May 8. Epub 2018 May 8.

Dept. of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Different phylogenetic groups (A, B1, B2 and D) through possess four functional groups; (I) , (II) , (III) , and (IV) can be caused urinary tract infections. Special phylogenetic group with high virulence content become a worldwide health concern. Resistance to antimicrobial agents increasingly complicates the management of extraintestinal infections due to , which are a major source of illness, death, and increased healthcare costs. Read More

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Fecal carriage of antimicrobial-resistant in healthy Korean adults.

J Microbiol Biotechnol 2018 May 8. Epub 2018 May 8.

Department of Molecular Cell Biology and Samsung Medical Center, Sungkyunkwan University School of Medicine, Suwon, Korea.

The spread of antibiotic-resistant the community is one of the main challenges for antibiotic treatment of community-onset infections. We evaluated the microbiological and molecular characteristics of stool samples from adults with comprehensive health examinations. Of 109 fecal samples, bacterial growth was observed in 86 samples and 61 Gram-negative bacterial isolates were identified, of which 45 were isolates (73. Read More

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In vitro Activity of Sitafloxacin and Other Antibiotics against Bacterial Isolates from HRH Princess Maha Chakri Sirindhorn Medical Center, Srinakharinwirot University and Samitivej Sukhumvit Hospital.

J Med Assoc Thai 2017 Apr;100(4):469-78

Background: Sitafloxacin is a newly approved oral fluoroquinolone in Thailand for treatment of respiratory tract and urinary tract infections. Initial in vitro susceptibility testing showed its effect on Escherichia coli with extended-spectrum betalactamases (ESBL), Klebsiella pneumoniae with ESBL, Pseudomonas aeruginosa, and carbapenem resistant Acinetobacter baumannii

Objective: To retrospectively review in vitro susceptibility to sitafloxacin on clinical isolates from HRH Princess Maha Chakri Sirindhorn Medical Center, Srinakharinwirot University (SWU) and Samitivej Sukhumvit Hospital (SVH).

Material And Method: Between January 2014 and June 2015, all clinical isolates from SWU and SVH that were added to test in vitro susceptibility to sitafloxacin were included in the present study. Read More

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A retrospective study on mcr-1 in clinical Escherichia coli and Klebsiella pneumoniae isolates in China from 2007 to 2016.

J Antimicrob Chemother 2018 Apr 17. Epub 2018 Apr 17.

Institute of Clinical Pharmacology, Peking University First Hospital, Beijing, China.

Objectives: To evaluate the prevalence of clinical mcr-1-positive Escherichia coli and Klebsiella pneumoniae and characterize the antimicrobial resistance profiles of mcr-1-positive E. coli and mcr-1-negative E. coli in China. Read More

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April 2018
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Insights into mechanism and functional consequences of heme binding to hemolysin-activating lysine acyltransferase HlyC from Escherichia coli.

Biochim Biophys Acta 2018 Jun 14. Epub 2018 Jun 14.

Pharmaceutical Biochemistry and Bioanalytics, Pharmaceutical Institute, University of Bonn, 53121 Bonn, Germany. Electronic address:

Background: Tight regulation of heme homeostasis is a critical mechanism in pathogenic bacteria since heme functions as iron source and prosthetic group, but is also toxic at elevated concentrations. Hemolysin-activating lysine-acyltransferase (HlyC) from Escherichia coli is crucial for maturation of hemolysin A, which lyses several mammalian cells including erythrocytes liberating large amounts of heme for bacterial uptake. A possible impact and functional consequences of the released heme on events employing bacterial HlyC have remained unexplored. Read More

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June 2018
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Action mechanism of melittin-derived antimicrobial peptides, MDP1 and MDP2, de novo designed against multidrug resistant bacteria.

Amino Acids 2018 Jun 15. Epub 2018 Jun 15.

Venom and Biotherapeutics Molecules Lab, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.

The emergence and dissemination of multidrug resistant (MDR) bacteria are major challenges for antimicrobial chemotherapy of bacterial infections. In this critical condition, cationic antimicrobial peptides are 'novel' promising candidate antibiotics to overcome the issue. In this study, we investigated the antibacterial mechanism of new melittin-derived peptides (i. Read More

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June 2018
3 Reads

Longer Duration of Urinary Catheterization Increases Catheter-Associated Urinary Tract Infection in PICU.

Pediatr Crit Care Med 2018 Jun 13. Epub 2018 Jun 13.

Division of Infectious Diseases, Department of Pediatrics, Tokyo Metropolitan Children's Medical Center, Tokyo, Japan.

Objectives: Catheter-associated urinary tract infections account for 30% of healthcare-associated infections. To date, few studies have addressed pediatric catheter-associated urinary tract infection in PICUs. The aim of our study was to assess the risk of catheter-associated urinary tract infection in relation to the duration of catheterization in the PICU. Read More

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The SiLuDrain Trial: a prospective randomized controlled trial comparing standard versus silver-impregnated lumbar drains.

J Neurosurg 2018 Jun 15:1-8. Epub 2018 Jun 15.

Department of Neurosurgery and.

OBJECTIVE Catheter-associated cerebrospinal fluid (CSF) infection remains a serious event, especially for patients in neurocritical care units. The use of external ventricular drain (EVD) catheters impregnated with antimicrobial substances has led to a significant reduction of infection rates. This study was undertaken to compare the use of antimicrobial, silver-impregnated external lumbar drains (si-ELDs) and conventional ELDs. Read More

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June 2018
2 Reads

Toxins of Locus of Enterocyte Effacement-Negative Shiga Toxin-Producing .

Toxins (Basel) 2018 Jun 14;10(6). Epub 2018 Jun 14.

Department of Food Microbiology and Hygiene, Institute of Food Science and Biotechnology, Garbenstrasse 28, University of Hohenheim, 70599 Stuttgart, Germany.

Studies on Shiga toxin-producing (STEC) typically examine and classify the virulence gene profiles based on genomic analyses. Among the screened strains, a subgroup of STEC which lacks the locus of enterocyte effacement (LEE) has frequently been identified. This raises the question about the level of pathogenicity of such strains. Read More

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Outbreak of E. coli O157:H7 Infections Associated with Exposure to Animal Manure in a Rural Community - Arizona and Utah, June-July 2017.

MMWR Morb Mortal Wkly Rep 2018 Jun 15;67(23):659-662. Epub 2018 Jun 15.

On June 26, 2017, a hospital in southern Utah notified the Utah Department of Health of Shiga toxin-producing Escherichia coli (STEC) O157:H7 infections in two children from a small community on the Arizona-Utah border. Both children developed hemolytic uremic syndrome, characterized by hemolytic anemia, acute kidney failure, and thrombocytopenia and died within a few days of illness onset. Over the next few days, several more STEC-associated illnesses were reported in residents of the community. Read More

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June 2018
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Escherichia coli and Staphylococcus aureus: leading bacterial pathogens of health-care associated infections and bacteremia at older age.

Expert Rev Vaccines 2018 Jun 14. Epub 2018 Jun 14.

b Vaccine Research and Development , Pfizer Inc . 401 N. Middletown Road, Pearl River, NY 10965 , USA .

Introduction: Bacteremia and healthcare-associated infections (HAI) continue to rise in industrialized countries, in line with population ageing and increased healthcare needs. Escherichia coli and Staphylococcus aureus are the most common pathogens causing HAI and bacteremia. Areas covered: This review discusses risk factors in the healthcare setting contributing to the rise in bacteremia and other invasive bacterial HAI in older populations in general, and attributed to E. Read More

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Data showing levels of interleukin-1β and nitric oxide in the plasma of uropathogenic infected UTI patients.

Data Brief 2018 Aug 18;19:526-529. Epub 2018 May 18.

Dr. B. R. Ambedkar Center for Biomedical Research (ACBR), University of Delhi, Delhi 110007, India.

Urinary tract infections (UTI) are a major cause of morbidity, affecting at least four million women worldwide, 65-75% of these infections are caused by Uropathogenic (UPEC) (Foxman, 2010) [1]. Repertoire of virulence factors carried by UPEC provides the ability to precede urinary tract and additionally they provoke pro-inflammatory responses (Cirl et al., 2008; Verma et al. Read More

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August 2018
3 Reads

Combinatorial drug discovery in nanoliter droplets.

Proc Natl Acad Sci U S A 2018 Jun 13. Epub 2018 Jun 13.

Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139;

Combinatorial drug treatment strategies perturb biological networks synergistically to achieve therapeutic effects and represent major opportunities to develop advanced treatments across a variety of human disease areas. However, the discovery of new combinatorial treatments is challenged by the sheer scale of combinatorial chemical space. Here, we report a high-throughput system for nanoliter-scale phenotypic screening that formulates a chemical library in nanoliter droplet emulsions and automates the construction of chemical combinations en masse using parallel droplet processing. Read More

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LMB-1, a novel family of class B3 MBLs from an isolate of Enterobacter cloacae.

J Antimicrob Chemother 2018 Jun 11. Epub 2018 Jun 11.

Department for Infection Control and Infectious Diseases, University Medical Center Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany.

Objectives: To identify and characterize a novel MBL gene conferring carbapenem resistance to an isolate of Enterobacter cloacae from Austria.

Methods: The novel MBL gene was heterologously expressed in Escherichia coli TOP10 to conduct comparative MIC studies and biochemical assays. Furthermore, WGS was performed using Illumina MiSeq and Oxford Nanopore MinION instruments to analyse the genetic environment of the novel MBL gene. Read More

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June 2018
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Complete genomic characterization of two Escherichia coli lineages responsible for a cluster of carbapenem-resistant infections in a Chinese hospital.

J Antimicrob Chemother 2018 Jun 11. Epub 2018 Jun 11.

Institute of Microbiology and Infection, College of Medical and Dental Science, University of Birmingham, Birmingham B15 2TT, UK.

Objectives: The increase in infections as a result of MDR strains of Escherichia coli is a global health crisis. The emergence of globally disseminated lineages of E. coli carrying ESBL genes has been well characterized. Read More

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June 2018
3 Reads

4-Thiazolidinone coumarin derivatives as two-component NS2B/NS3 DENV flavivirus serine protease inhibitors: synthesis, molecular docking, biological evaluation and structure-activity relationship studies.

Chem Cent J 2018 Jun 12;12(1):69. Epub 2018 Jun 12.

School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia.

A series of novel 4-thiazolidinone inhibitors SKYa-SKYg, containing coumarin as a core structure were synthesized via facile and efficient method. The structures of the synthesized compounds were established by extensive spectroscopic studies (FT IR, 1D NMR, 2D NMR, LC-MS) and elemental analysis. All the synthesized hybrids were further evaluated for their potential as anti-tubercular agents against Mycobacterium tuberculosis H37Rv ATCC 25618, and anti-bacterial agents against Escherichia coli, Enterobacter aerogenes, Salmonella typhi, Streptococcus pneumoniae and Staphylococcus aureus. Read More

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June 2018
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Detection of Antimicrobial Susceptibility and Integrons Among Extended-spectrum β-lactamase Producing Uropathogenic Isolates in Southwestern Iran.

Oman Med J 2018 May;33(3):218-223

Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Objectives: Urinary tract infections (UTIs) are one of the most prevalent infectious diseases and can lead to a high rate of morbidity and mortality. The emergence of multiple-drug resistant strains, particularly extended-spectrum beta-lactamases (ESBLs) producing strains, has become a global healthcare concern. Our study sought to investigate the antimicrobial resistance pattern and presence of integrons and determinants among uropathogenic (UPEC) isolates obtained from hospitalized Iranian patients. Read More

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The Lectin Complement Pathway Is Involved in Protection Against Enteroaggregative Infection.

Front Immunol 2018 29;9:1153. Epub 2018 May 29.

Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, University Hospital of Copenhagen, Copenhagen, Denmark.

Enteroaggregative (EAEC) causes acute and persistent diarrhea worldwide. Still, the involvement of host factors in EAEC infections is unresolved. Binding of recognition molecules from the lectin pathway of complement to EAEC strains have been observed, but the importance is not known. Read More

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May 2018
2 Reads

Reassessing the Role of Type II Toxin-Antitoxin Systems in Formation of Escherichia coli Type II Persister Cells.

MBio 2018 Jun 12;9(3). Epub 2018 Jun 12.

Cellular and Molecular Microbiology (CM2), Faculté des Sciences, Université Libre de Bruxelles (ULB), Gosselies, Belgium

Persistence is a reversible and low-frequency phenomenon allowing a subpopulation of a clonal bacterial population to survive antibiotic treatments. Upon removal of the antibiotic, persister cells resume growth and give rise to viable progeny. Type II toxin-antitoxin (TA) systems were assumed to play a key role in the formation of persister cells in based on the observation that successive deletions of TA systems decreased persistence frequency. Read More

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Emergence of mcr-1 plasmid-mediated colistin-resistant Escherichia coli isolates from seawater.

Sci Total Environ 2018 Jun 9;642:90-94. Epub 2018 Jun 9.

Unité Environnement, Institut Pasteur d'Algérie, Alger 16000, Algeria.

Colistin is currently regarded as one of the 'last-resort' antibiotics used for the treatment of critical infections caused by multidrug-resistant Gram-negative pathogens. Recently, there have been numerous reports of the emergence of a transferable plasmid-mediated colistin resistance gene, mcr-1 in patients, animals, food, and environment. Here, we characterize the support of colistin resistance among environmental isolates collected from seawater of Algiers coast. Read More

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June 2018
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Complete genome sequence of uropathogenic Escherichia coli isolate UPEC 26-1.

Genes Genomics 2018 Jun 14;40(6):643-655. Epub 2018 Feb 14.

Department of Microbiology and Medical Science, Chungnam National University School of Medicine, 266 Munwha-ro, Jung-gu, Daejeon, 35015, Republic of Korea.

Urinary tract infections (UTIs) are among the most common infections in humans, predominantly caused by uropathogenic Escherichia coli (UPEC). The diverse genomes of UPEC strains mostly impede disease prevention and control measures. In this study, we comparatively analyzed the whole genome sequence of a highly virulent UPEC strain, namely UPEC 26-1, which was isolated from urine sample of a patient suffering from UTI in Korea. Read More

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Virulence properties of uropathogenic Escherichia coli isolated from children with urinary tract infection in Korea.

Genes Genomics 2018 Jun 14;40(6):625-634. Epub 2018 Feb 14.

Department of Microbiology and Medical Science, Chungnam National University School of Medicine, 266 Munwha-ro, Jung-gu, Daejeon, 35015, Republic of Korea.

Urinary tract infections (UTIs) are one of the most common types of bacterial infection in humans in various parts of the world and are caused mainly by uropathogenic Escherichia coli (UPEC). A total of 58 UPEC isolates from urine were characterized by serotyping and pulsed-field gel electrophoresis (PFGE). The majority of the UPEC strains belonged to serogroups O2 and O6. Read More

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Selected microRNA-192 mutant indicates association with several function genes in bovine cells.

Genes Genomics 2018 Apr 14;40(4):361-371. Epub 2017 Dec 14.

Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, MD, USA.

MicroRNAs are implicated in many cellular processes such as cell differentiation and development, tumorigenesis, and immune regulation. In this study, miR192 was detected using quantitative real-time polymerase chain reaction (qRT-PCR) when MDBK cells were exposed to Escherichia coli. Cells with malfunction of bta-miR-192 were established using transcription activator-like effector nuclease (TALEN) technology. Read More

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Immunomodulation of Host Chitinase 3-Like 1 During a Mammary Pathogenic Infection.

Front Immunol 2018 28;9:1143. Epub 2018 May 28.

Laboratory of Biochemistry, Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

Chitin is a -acetyl-d-glucosamine biopolymer that can be recognized by chitin-binding proteins. Although mammals lack chitin synthase, they induce proteins responsible for detecting chitin in response to bacterial infections. Our aim was to investigate whether chitinase 3-like 1 (CHI3L1) has a potential role in the innate immunity of the () infected mammary gland. Read More

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May 2018
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Urine cytokine and chemokine levels predict urinary tract infection severity independent of uropathogen, urine bacterial burden, host genetics, and host age.

Infect Immun 2018 Jun 11. Epub 2018 Jun 11.

Department of Microbiology and Immunology; University of Michigan Medical School; Ann Arbor, MI 48109; United States.

Urinary tract infections (UTIs) are among the most common infections worldwide. Diagnosing UTIs in older adults poses a significant challenge as asymptomatic colonization is common. Identification of a non-invasive profile that predicts likelihood of progressing from urine colonization to severe disease would provide a significant advantage in clinical practice. Read More

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Evaluation of the Antimicrobial Activity and Cytotoxicity of Different Components of Natural Origin Present in Essential Oils.

Molecules 2018 Jun 8;23(6). Epub 2018 Jun 8.

Department of Chemical and Environmental Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Río Ebro-Edificio I+D, C/Poeta Mariano Esquillor S/N, 50018 Zaragoza, Spain.

Even though essential oils (EOs) have been used for therapeutic purposes, there is now a renewed interest in the antimicrobial properties of phytochemicals and EOs in particular. Their demonstrated low levels of induction of antimicrobial resistance make them interesting for bactericidal applications, though their complex composition makes it necessary to focus on the study of their main components to identify the most effective ones. Herein, the evaluation of the antimicrobial action of different molecules present in EOs against planktonic and biofilm-forming Gram-positive () and Gram-negative () bacteria was assessed. Read More

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Genomic Insertion of a Heterologous Acetyltransferase Generates a New Lipopolysaccharide Antigenic Structure in and .

Front Microbiol 2018 25;9:1092. Epub 2018 May 25.

Instituto de Salud Tropical, Instituto de Investigación Sanitaria de Navarra, Departamento de Microbiología y Parasitología, Universidad de Navarra, Pamplona, Spain.

Brucellosis is a bacterial zoonosis of worldwide distribution caused by bacteria of the genus . In and , the major species infecting domestic ruminants, the smooth lipopolysaccharide (S-LPS) is a virulence factor. This S-LPS carries a -formyl-perosamine homopolymer -polysaccharide that is the major antigen in serodiagnostic tests and is required for virulence. Read More

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Real-time assessment of bacteriophage T3-derived antimicrobial activity against planktonic and biofilm-embedded Escherichia coli by isothermal microcalorimetry.

Res Microbiol 2018 Jun 7. Epub 2018 Jun 7.

Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany; Berlin-Brandenburg Center for Regenerative Therapies Charité - University Medicine Berlin, Augustenburger Platz 1 (Südstraße 2) 13353 Berlin, Germany.

Bacterial biofilms, highly resistant to the conventional antimicrobial therapy, remain an unresolved challenge pressing the medical community to investigate new and alternative strategies to fight chronic implant-associated infections. Recently, strictly lytic bacteriophages have been revalued as powerful agents to kill antibiotic-resistant bacteria even in biofilm. Here, the interaction of T3 bacteriophage and planktonic and biofilm Escherichia coli TG1, respectively, was evaluated using isothermal microcalorimetry. Read More

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Toxic epidermal necrolysis data from the CELESTE multinational registry. Part I: Epidemiology and general microbiological characteristics.

Burns 2018 Jun 6. Epub 2018 Jun 6.

Department of Burns and Reconstructive Surgery, Teaching Hospital Brno, Czech Republic; Faculty of Medicine, Masaryk University Brno, Czech Republic.

Introduction: Toxic epidermal necrolysis (TEN) is a rare, life-threatening autoimmune disease predominantly manifested in the skin and mucous membranes. Today, infectious complications have the dominant share in mortality of TEN patients. Due to the nature of the therapy and administration of immunosuppressive medications, a wide range of potentially pathogenic microorganisms, which cause infectious complications in different compartments in these patients, is not surprising. Read More

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Near-infrared-triggered antibacterial and antifungal photodynamic therapy based on lanthanide-doped upconversion nanoparticles.

Nanoscale 2018 Jun 8. Epub 2018 Jun 8.

State Key Laboratory of Structural Chemistry, CAS Key Laboratory of Design and Assembly of Functional Nanostructures, and Fujian Key Laboratory of Nanomaterials, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China. and University of Chinese Academy of Sciences, Beijing 100049, China.

An alarming worldwide increase in microbial resistance to traditional drugs and classical pharmacophores has spurred the search for new antimicrobial compounds. Antimicrobial photodynamic therapy (aPDT) has recently emerged as an effective modality for the selective destruction of bacteria and other pathogenic microorganisms. However, some of the factors, including the aggregation of the hydrophobic photosensitizer (PS) in aqueous media and the inefficient biodistribution of PS limit its expansion to clinical conditions. Read More

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June 2018
2 Reads

A novel nitroreductase-enhanced MRI contrast agent and its potential application in bacterial imaging.

Acta Pharm Sin B 2018 May 6;8(3):401-408. Epub 2017 Dec 6.

State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China.

Nitroreductases (NTRs) are known to be able to metabolize nitro-substituted compounds in the presence of reduced nicotinamide adenine dinucleotide (NADH) as an electron donor. NTRs are present in a wide range of bacterial genera and, to a lesser extent, in eukaryotes hypoxic tumour cells and tumorous tissues, which makes it an appropriate biomarker for an imaging target to detect the hypoxic status of cancer cells and potential bacterial infections. To evaluate the specific activation level of NTR, great efforts have been devoted to the development of fluorescent probes to detect NTR activities using fluorogenic methods to probe its behaviour in a cellular context; however, NTR-responsive MRI contrast agents are still by far underexplored. Read More

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Bacteriological profile and antimicrobial susceptibility patterns of urine culture isolates from patients in Ndjamena, Chad.

Pan Afr Med J 2017 23;28:258. Epub 2017 Nov 23.

Department of Medical Microbiology and Immunology, School of Health Sciences-Catholic University of Central Africa, Yaoundé, Cameroon.

Introduction: Bacteriological profile and antimicrobial susceptibility patterns of urine culture isolates were determined among patients in the Ndjamena General Hospital, a National Reference centre.

Methods: A cross-sectional study was carried out from July to November 2014. Six hundred and sixty patients were enrolled, to whom a cytobacteriological examination of urine was prescribed. Read More

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June 2018
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