57,010 results match your criteria Escherichia Coli Infections


Does concomitant bacteraemia hide the fungi in blood cultures? An in vitro study.

J Med Microbiol 2020 Jun 3. Epub 2020 Jun 3.

Department of Microbiology, Faculty of Medicine, University of Eskisehir Osmangazi, Eskisehir, Turkey.

Polymicrobial infections including yeasts and bacteria are not rare and patients with polymicrobial bloodstream infection have higher early and overall case fatality rates. The diagnosis of invasive fungal and bacterial infections is mainly based on blood culture. The aim was to reveal the effect of concomitant bacteraemia on the detection of fungi from blood cultures in the presence of polymicrobial bloodstream infections involving and non- fungi and to show the superiority of blood culture bottles including selective fungal media in such situations. Read More

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http://dx.doi.org/10.1099/jmm.0.001210DOI Listing

co-infection with Enterohemorrhagic (EHEC)-a potentially fatal combination.

IDCases 2020 8;21:e00797. Epub 2020 May 8.

Department of Gastroenterology, Bassett Medical Center, New York, USA.

There has been a significant increase in hospital and community acquired infection in the past decade. In addition to CDI, the rate of infection with enterohemorrhagic (EHEC) has also increased by 28% in United States in the last five years. Concomitant CDI and EHEC infections are rare and if not identified early such co-infections can be fatal. Read More

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http://dx.doi.org/10.1016/j.idcr.2020.e00797DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256656PMC

The influence of dietary supplementation with the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on the chemotaxis, phagocytosis and respiratory burst of peripheral blood granulocytes and monocytes in calves.

BMC Vet Res 2020 Jun 1;16(1):171. Epub 2020 Jun 1.

Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719, Olsztyn, Poland.

Background: A healthy immune system plays a particularly important role in newborns, including in calves that are far more susceptible to infections (viral, bacterial and other) than adult individuals. Therefore, the present study aimed to evaluate the influence of HMB on the chemotactic activity (MIGRATEST® kit), phagocytic activity (PHAGOTEST® kit) and oxidative burst (BURSTTEST® kit) of monocytes and granulocytes in the peripheral blood of calves by flow cytometry.

Results: An analysis of granulocyte and monocyte chemotactic activity and phagocytic activity revealed significantly higher levels of phagocytic activity in calves administered HMB than in the control group, expressed in terms of the percentage of phagocytising cells and mean fluorescence intensity (MFI). Read More

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http://dx.doi.org/10.1186/s12917-020-02389-1DOI Listing

Virulence Potential of a Multidrug-Resistant Strain Belonging to the Emerging Clonal Group ST101-B1 Isolated from Bloodstream Infection.

Microorganisms 2020 May 30;8(6). Epub 2020 May 30.

Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia-DMIP, Universidade Federal de São Paulo-Escola Paulista de Medicina (UNIFESP-EPM), São Paulo-SP 04023-062, Brazil.

Escherichia coli EC121 is a multidrug-resistant (MDR) strain isolated from a bloodstream infection of an inpatient with persistent gastroenteritis and T-zone lymphoma that died due to septic shock. Despite causing an extraintestinal infection, previous studies showed that it did not have the usual characteristics of an extraintestinal pathogenic Instead, it belonged to phylogenetic group B1 and harbored few known virulence genes. To evaluate the pathogenic potential of strain EC121, an extensive genome sequencing and in vitro characterization of various pathogenicity-associated properties were performed. Read More

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http://dx.doi.org/10.3390/microorganisms8060827DOI Listing

Chalcone coating on cotton cloth - an approach to reduce attachment of live microbes.

Biomater Sci 2014 Jul 26;2(7):990-995. Epub 2014 Feb 26.

Department of Biotechnology, Indian Institute of Technology Madras, Adyar, Chennai 600036, India.

Drug resistant bacteria are a major threat to humans, especially those which mediate nosocomial infections. In this paper, three different 4-sulfonylmethyl chalcones are coated onto cotton cloths with acacia as the binder using a padding mangle to make them antibacterial. A 99% reduction in the adhesion of three slime producing organisms, namely Staphylococcus aureus NCIM5021, Escherichia coli NCIM2931 and Pseudomonas aeruginosa NCIM2901, on these surfaces was observed. Read More

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http://dx.doi.org/10.1039/c3bm60312aDOI Listing

Phage-delivered sensitisation with subsequent antibiotic treatment reveals sustained effect against antimicrobial resistant bacteria.

Theranostics 2020 15;10(14):6310-6321. Epub 2020 May 15.

Academy of Military Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, China.

Temperate phages integrated with clustered regularly interspaced short palindromic repeat (CRISPR)/Cas systems have been gaining attention as potential strategies for combating bacteria resistant to antimicrobials. To further advance this technology, phage recombination procedure should be improved, and the bactericidal effect should be examined in detail and compared with conventional lytic phage strategy. The possibility of the emergence of mutational resistance, a phenomenon commonly observed with lytic phage therapy, should be illustrated. Read More

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http://dx.doi.org/10.7150/thno.42573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255016PMC

Colibactin DNA-damage signature indicates mutational impact in colorectal cancer.

Nat Med 2020 Jun 1. Epub 2020 Jun 1.

Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany.

The mucosal epithelium is a common target of damage by chronic bacterial infections and the accompanying toxins, and most cancers originate from this tissue. We investigated whether colibactin, a potent genotoxin associated with certain strains of Escherichia coli, creates a specific DNA-damage signature in infected human colorectal cells. Notably, the genomic contexts of colibactin-induced DNA double-strand breaks were enriched for an AT-rich hexameric sequence motif, associated with distinct DNA-shape characteristics. Read More

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http://dx.doi.org/10.1038/s41591-020-0908-2DOI Listing

Bacteriophage K1F targets Escherichia coli K1 in cerebral endothelial cells and influences the barrier function.

Sci Rep 2020 Jun 1;10(1):8903. Epub 2020 Jun 1.

School of Life Sciences, University of Warwick, Gibbet Hill Road, CV4 7AL, Coventry, UK.

Bacterial neonatal meningitis results in high mortality and morbidity rates for those affected. Although improvements in diagnosis and treatment have led to a decline in mortality rates, morbidity rates have remained relatively unchanged. Bacterial resistance to antibiotics in this clinical setting further underlines the need for developing other technologies, such as phage therapy. Read More

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http://dx.doi.org/10.1038/s41598-020-65867-4DOI Listing

The limited knowledge of placental damage due to neglected infections: ongoing problems in Latin America.

Syst Biol Reprod Med 2020 Jun;66(3):151-169

Laboratory of Vascular Biology and Histopathology, Institute of Health Sciences and Health, Federal University of Mato Grosso , Barra Do Garcas, Brazil.

The placenta works as a selective barrier, protecting the fetus from potential infections that may affect the maternal organism during pregnancy. In this review, we will discuss several challenging infections that are common within Latin American countries and that may affect the maternal-fetal interface and pose risks to fetal development. Specifically, we will focus on emerging infectious diseases including the arboviruses, malaria, leishmaniasis, and the bacterial foodborne disease caused by Shiga toxin-producing . Read More

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http://dx.doi.org/10.1080/19396368.2020.1753850DOI Listing

Unsymmetrical cationic porphyrin-cyclodextrin bioconjugates for photoinactivation of Escherichia coli.

Photodiagnosis Photodyn Ther 2020 May 29:101788. Epub 2020 May 29.

LAQV-REQUIMTE and Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address:

Photodynamic inactivation (PDI) of microorganisms have been used for the treatment of bacterial infections. PDI is based on the combination of three non-toxic elements: a photosensitizer (PS), light and molecular oxygen, which lead to the formation of reactive oxygen species (ROS) that cause lethal oxidative damage into the target pathogenic bacteria. For that, clinical approved tetrapyrrolic macrocycles, with particular emphasis on photoactive porphyrin (Por) dyes, which have been used as PS in PDI for different biomedical applications. Read More

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http://dx.doi.org/10.1016/j.pdpdt.2020.101788DOI Listing

Characterization of ESBL/pAmpC-producing Escherichia coli isolates from long-term recurrent bloodstream infections.

Int J Antimicrob Agents 2020 May 29:106041. Epub 2020 May 29.

Department of Bacteria, Parasites & Fungi, Infectious Disease Preparedness, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark; Department of Cellular and Molecular Medicine, Center for Peptide-Based Antibiotics, The Panum Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark.

The aim of the study was to investigate recurrent infections within individual patients caused by Extended-spectrum beta-lactamase and plasmid-AmpC-producing Escherichia coli (ESBL/pAmpC Ec) isolates, with more than one year interval. The national collection of ESBL/pAmpC Ec isolates collected from January 2014 through June 2017, where screened for patients with multiple isolates with more than one year between the episodes. The isolates were whole genome sequenced and analysed for resistance genes, virulence genes and MLST. Read More

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http://dx.doi.org/10.1016/j.ijantimicag.2020.106041DOI Listing

Widespread high-risk clones of multidrug-resistant ESBL-producing Escherichia coli B2-ST131 and F-ST648 in public aquatic environments.

Int J Antimicrob Agents 2020 May 29:106040. Epub 2020 May 29.

Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto - Universidade de São Paulo (USP) - Ribeirão Preto, Brasil. Electronic address:

Aquatic environments are considered reservoir and disseminators of multidrug-resistant (MDR) bacteria, principally Escherichia coli, with the consequent spread of acquired antimicrobial resistance genes (ARGs) and widespread high-risk clones of MDR E. coli are responsible for human infections worldwide. This study aimed to characterize through whole-genome sequencing (WGS) MDR ARGs-producing E. Read More

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http://dx.doi.org/10.1016/j.ijantimicag.2020.106040DOI Listing

Application of Oleanolic Acid and Its Analogues in Combating Pathogenic Bacteria / by a Two-Pronged Strategy of β-Lactamases and Hemolysins.

ACS Omega 2020 May 12;5(20):11424-11438. Epub 2020 May 12.

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.

The rapid spread of β-lactamase-producing bacteria in clinical practice has increasingly deteriorated the performance of β-lactam antibiotics against such resistant strains. Thus, novel agents or strategies for the war against β-lactamase-producing bacteria, especially hypervirulent resistant bacteria (such as toxin-secreting ) carrying complex β-lactamases, are urgently needed. In this study, we found that the natural compound oleanolic acid (OA) and its analogues (especially corosolic acid (CA)) significantly inhibited the activity of important β-lactamases (NDM-1, KPC-2, and VIM-1) in Enterobacteriaceae and β-lactamases (β-lactamase N1) in . Read More

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http://dx.doi.org/10.1021/acsomega.0c00460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254530PMC

Effect of bismuth subnitrate on in vitro growth of major mastitis pathogens.

J Dairy Sci 2020 May 28. Epub 2020 May 28.

School of Veterinary Science, Massey University, Palmerston North 4410, New Zealand.

The mode of action of bismuth subnitrate in teat sealant formulations as a preventative for intramammary infections during the dry period is unknown. Although previous studies proposed an action mechanism-creating a physical barrier in the teat canal to prevent bacterial invasion-it has not been proven experimentally. We hypothesized that bismuth subnitrate has an inhibitory effect on bacterial growth, in addition to its barrier effect. Read More

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http://dx.doi.org/10.3168/jds.2019-17830DOI Listing

In Vitro Activity of Aztreonam-Avibactam against Metallo-β-lactamase producing Enterobacteriaceae - a Multi-center study in China.

Int J Infect Dis 2020 May 27. Epub 2020 May 27.

Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. Electronic address:

Objectives: To study the molecular epidemiology of clinical MBL-Enterobacteriaceae isolates in China and to evaluate the antimicrobial susceptibility of aztreonam-avibactam against MBL-Enterobacteriaceae isolates.

Methods: Bacterial speciation was determined using the MALDI-TOF MS apparatus. PCR was used to screen common carbapenemase genes. Read More

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http://dx.doi.org/10.1016/j.ijid.2020.05.075DOI Listing

Development and selection of low-level multi-drug resistance over an extended range of sub-inhibitory ciprofloxacin concentrations in Escherichia coli.

Sci Rep 2020 May 29;10(1):8754. Epub 2020 May 29.

Boston University, Department of Biomedical Engineering, Boston, MA, USA.

To better combat bacterial antibiotic resistance, a growing global health threat, it is imperative to understand its drivers and underlying biological mechanisms. One potential driver of antibiotic resistance is exposure to sub-inhibitory concentrations of antibiotics. This occurs in both the environment and clinic, from agricultural contamination to incorrect dosing and usage of poor-quality medicines. Read More

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http://dx.doi.org/10.1038/s41598-020-65602-zDOI Listing

The Gut of Healthy Infants in the Community as a Reservoir of ESBL and Carbapenemase-Producing Bacteria.

Antibiotics (Basel) 2020 May 27;9(6). Epub 2020 May 27.

Department of Pediatrics, Duke University, 2301 Erwin Rd, Durham, NC 27710, USA.

The recent rapid rise of multi-drug resistant Enterobacteriaceae (MDR-E) is threatening the treatment of common infectious diseases. Infections with such strains lead to increased mortality and morbidity. Using a cross-sectional study, we aimed to estimate the prevalence of gut colonization with extended spectrum beta-lactamase (ESBL) producing Enterobacteriaceae among healthy infants born in Pakistan, a setting with high incidence of MDR-E infections. Read More

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http://dx.doi.org/10.3390/antibiotics9060286DOI Listing

Comparative Study of the Antimicrobial Effects of Tungsten Nanoparticles and Tungsten Nanocomposite Fibres on Hospital Acquired Bacterial and Viral Pathogens.

Nanomaterials (Basel) 2020 May 26;10(6). Epub 2020 May 26.

Department of Mechanical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.

A significant proportion of patients acquire hospital associated infections as a result of care within the NHS each year. Numerous antimicrobial strategies, such as antibiotics and surface modifications to medical facilities and instruments, have been devised in an attempt to reduce the incidence of nosocomial infections, but most have been proven unsuccessful and unsustainable due to antibiotic resistance. Therefore, the need to discover novel materials that can combat pathogenic microorganisms is ongoing. Read More

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http://dx.doi.org/10.3390/nano10061017DOI Listing

Analysis of Susceptibility to Selected Antibiotics in and Causing Urinary Tract Infections in Kidney Transplant Recipients over 8 Years: Single-Center Study.

Antibiotics (Basel) 2020 May 26;9(6). Epub 2020 May 26.

Department of Transplantation Medicine, Nephrology, Internal Diseases, T. Orłowski Institute of Transplantation, Medical University of Warsaw, 59 Nowogrodzka Street, 02-006 Warsaw, Poland.

Urinary tract infections (UTIs) are the most common bacterial infections among kidney transplant (KTX) recipients. The purpose of this study was to analyze antimicrobial resistance (AMR) in four most common pathogens responsible for UTIs in KTX recipients and determine risk factors (RF) for resistance in the same group. Analyzed antibiograms were based on urine samples positive for bacterial growth of 10 colony-forming units (CFU)/mL obtained from hospitalized adult KTX recipients presenting with UTI symptoms upon admission to the center in years 2011-2018. Read More

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http://dx.doi.org/10.3390/antibiotics9060284DOI Listing

Natural Cyanobacterial Polymer-Based Coating as a Preventive Strategy to Avoid Catheter-Associated Urinary Tract Infections.

Mar Drugs 2020 May 26;18(6). Epub 2020 May 26.

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal.

Catheter-associated urinary tract infections (CAUTIs) represent about 40% of all healthcare-associated infections. Herein, the authors report the further development of an infection preventive anti-adhesive coating (CyanoCoating) meant for urinary catheters, and based on a natural polymer released by a marine cyanobacterium. CyanoCoating performance was assessed against relevant CAUTI etiological agents, namely Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae, methicillin resistant Staphylococcus aureus (MRSA), and Candida albicans in the presence of culture medium or artificial urine, and under biofilm promoting settings. Read More

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http://dx.doi.org/10.3390/md18060279DOI Listing
May 2020
2.853 Impact Factor

Antimicrobial and Antifungal Activities of Terpene-Derived Palladium Complexes.

Antibiotics (Basel) 2020 May 25;9(5). Epub 2020 May 25.

Community for Open Antimicrobial Drug Discovery (CO-ADD), Centre for Superbug Solutions, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia.

In an era of multidrug-resistant bacterial infections overshadowed by a lack of innovation in the antimicrobial drug development pipeline, there has been a resurgence in multidisciplinary approaches aimed at tackling this global health problem. One such approach is to use metal complexes as a framework for new antimicrobials. Indeed, in this context, bismuth-, silver- and gold-derived compounds in particular have displayed demonstrable antimicrobial activity. Read More

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http://dx.doi.org/10.3390/antibiotics9050277DOI Listing

In Vitro Activity of Essential Oils Against Planktonic and Biofilm Cells of Extended-Spectrum β-Lactamase (ESBL)/Carbapenamase-Producing Gram-Negative Bacteria Involved in Human Nosocomial Infections.

Antibiotics (Basel) 2020 May 25;9(5). Epub 2020 May 25.

Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, Italy.

The aim of this study was to analyze the antibacterial activity of four essential oils (EOs), , , and in preventing the development and spread of extended-spectrum β-lactamase (ESBL)-producing and , metallo-beta-lactamase (MBL)-producing and carbapenemase (KPC)-producing . A total of 60 strains were obtained from the stock collection from the Microbiology Laboratory of Hesperia Hospital, Modena, Italy. Twenty ESBL-producing , 5 , 13 KPC-producing and 20 MBL-producing were cultured and reconfirmed as ESBL and carbapenamase producers. Read More

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http://dx.doi.org/10.3390/antibiotics9050272DOI Listing

Comparison of Escherichia coli antibiotic-resistance profiles and incidence of betalactamase phenotypes in three private health facilities in Perú

Biomedica 2020 05 1;40(Supl. 1):139-147. Epub 2020 May 1.

Laboratorio de Microbiología, Hospital Nacional Docente "Madre Niño San Bartolomé", Lima, Perú.

Introduction: The appearance of multidrug-resistant and beta-lactamase producing enterobacteria in outpatient care facilities represent a public health problem in Perú. Objective: To compare the resistance profiles of uropathogenic Escherichia coli and to identify extended-spectrum beta-lactamase-producing phenotypes in three private health facilities located in the Peruvian coast, Andean and jungle regions. Materials and methods: We conducted a descriptive study on 98 urine samples from Lima (coast), Juliaca (Andean region) and Iquitos (jungle region) during 2016. Read More

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http://dx.doi.org/10.7705/biomedica.4772DOI Listing

Horizontal Gene Transfer of Antibiotic Resistance from Acinetobacter baylyi to Escherichia coli on Lettuce and Subsequent Antibiotic Resistance Transmission to the Gut Microbiome.

mSphere 2020 May 27;5(3). Epub 2020 May 27.

Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA

Agricultural use of antibiotics is recognized by the U.S. Centers for Disease Control and Prevention as a major contributor to antibiotic-resistant infections. Read More

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http://dx.doi.org/10.1128/mSphere.00329-20DOI Listing

[Laboratory errors for identification of the escherichia coli strains of the serological groups O6 and O25 as acute intestinal infections.]

Klin Lab Diagn 2020 ;65(6):368-374

Saint-Petersburg Pasteur Institute, 197101, Saint-Petersburg, Russia.

Were studied the genes encoding the virulence factors of 221 strains: E. coli O:H (194) and E. coli O:H (27), isolated in 2014-2018 from stool samples of children and adults examined according to epidemic indications. Read More

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http://dx.doi.org/10.18821/0869-2084-2020-65-6-368-374DOI Listing
January 2020

bactericidal activity of a carbohydrate polymer with zinc oxide for the treatment of chronic wounds.

J Med Microbiol 2020 May 27. Epub 2020 May 27.

Direction of Research, Hospital Juárez de México, Cuidad de México, Mexico.

Biological adhesives and effective topical therapeutic agents that improve wound healing are urgently required for the treatment of chronic ulcers. A biodegradable adhesive based on a carbohydrate polymer with zinc oxide (CPZO) was shown to possess anti-inflammatory activity and enhance wound healing, but its bactericidal activity was unknown. To investigate the bactericidal activity of CPZO against bacteria commonly present as infectious agents in chronic wounds. Read More

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http://dx.doi.org/10.1099/jmm.0.001204DOI Listing

Etiology of Pediatric Meningitis in West Africa Using Molecular Methods in the Era of Conjugate Vaccines against Pneumococcus, Meningococcus, and Type b.

Am J Trop Med Hyg 2020 May 26. Epub 2020 May 26.

Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia.

Despite the implementation of effective conjugate vaccines against the three main bacterial pathogens that cause meningitis, , type b (Hib), and serogroup A, the burden of meningitis in West Africa remains high. The relative importance of other bacterial, viral, and parasitic pathogens in central nervous system infections is poorly characterized. Cerebrospinal fluid (CSF) specimens were collected from children younger than 5 years with suspected meningitis presenting at pediatric teaching hospitals across West Africa in five countries including Senegal, Ghana, Togo, Nigeria, and Niger. Read More

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http://dx.doi.org/10.4269/ajtmh.19-0566DOI Listing

Genomic analysis of fluoroquinolone-susceptible phylogenetic group B2 extraintestinal pathogenic Escherichia coli causing infections in cats.

Vet Microbiol 2020 Jun 18;245:108685. Epub 2020 Apr 18.

School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA, Australia; Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, SA, Australia.

Extraintestinal pathogenic Escherichia coli (ExPEC) can cause urinary tract and other types of infection in cats, but the relationship of cat ExPEC to human ExPEC remains equivocal. This study investigated the prevalence of ExPEC-associated sequence types (STs) from phylogenetic group B2 among fluoroquinolone-susceptible cat clinical isolates. For this, 323 fluoroquinolone-susceptible cat clinical E. Read More

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http://dx.doi.org/10.1016/j.vetmic.2020.108685DOI Listing

Molecular Biology of Shiga Toxins' Effects on Mammalian Cells.

Authors:
Christian Menge

Toxins (Basel) 2020 May 23;12(5). Epub 2020 May 23.

Friedrich-Loeffler-Institut/Federal Research Institute for Animal Health, Institute of Molecular Pathogenesis, Naumburger Str. 96a, D-07743 Jena, Germany.

Shiga toxins (Stxs), syn. Vero(cyto)toxins, are potent bacterial exotoxins and the principal virulence factor of enterohemorrhagic (EHEC), a subset of Shiga toxin-producing (STEC). EHEC strains, e. Read More

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http://dx.doi.org/10.3390/toxins12050345DOI Listing

Targeting the Early Endosome-to-Golgi Transport of Shiga Toxins as a Therapeutic Strategy.

Toxins (Basel) 2020 May 22;12(5). Epub 2020 May 22.

Division of Pharmacology & Toxicology, College of Pharmacy, Institute for Cellular & Molecular Biology, and Institute for Neuroscience, The University of Texas at Austin, Austin, TX 78712, USA.

Shiga toxin (STx) produced by and closely related Shiga toxin 1 and 2 (STx1 and STx2) synthesized by Shiga toxin-producing (STEC) are bacterial AB toxins. All three toxins target kidney cells and may cause life-threatening renal disease. While infections can be treated with antibiotics, resistance is increasing. Read More

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http://dx.doi.org/10.3390/toxins12050342DOI Listing

Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria.

Microorganisms 2020 May 22;8(5). Epub 2020 May 22.

Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de México, México City CP 04510, Mexico.

Background: and are etiologic agents of intestinal infections. A previous study showed the presence of shared epitopes between lipopolysaccharides (LPSs) of O157 and .

Aim: Using phage display, the aim of this study is to identify mimotopes of shared epitopes in different enterobacterial LPSs. Read More

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http://dx.doi.org/10.3390/microorganisms8050780DOI Listing

Application of phage therapy: Synergistic effect of phage EcSw (ΦEcSw) and antibiotic combination towards antibiotic-resistant Escherichia coli.

Transbound Emerg Dis 2020 May 26. Epub 2020 May 26.

College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon, 34134, Republic of Korea.

Bacteriophage therapy is acknowledged as a potential tool to prevent or treat multidrug resistant bacterial infections. In this study, our major focus was on the bacteriolytic activity of phage EcSw (ΦEcSw) against the emergence of the clinically important Escherichia coli Sw1 and E. coli O157:H7. Read More

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http://dx.doi.org/10.1111/tbed.13646DOI Listing

High Burden of Serious Bacterial Infections in African Children Treated for Cancer.

Pediatr Infect Dis J 2020 May 21. Epub 2020 May 21.

From the Medical Research Council: Respiratory and Meningeal Pathogens Research Unit, Paediatric Oncology Unit, Chris Hani Baragwanath Academic Hospital.

Background: Infections in children treated for cancer contribute to morbidity and mortality. There is a paucity of studies on the incidence, etiology, risk factors and outcome of bacterial infections in African children treated for cancer. The aim of the study was to delineate the epidemiology of infectious morbidity and mortality in children with cancer. Read More

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http://dx.doi.org/10.1097/INF.0000000000002758DOI Listing
May 2020
2.723 Impact Factor

Recombination of T4-like Phages and Its Activity against Pathogenic Escherichia coli in Planktonic and Biofilm Forms.

Virol Sin 2020 May 25. Epub 2020 May 25.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.

The increasing emergence of multi-drug resistant Escherichia coli (E. coli) has become a global concern, primarily due to the limitation of antimicrobial treatment options. Phage therapy has been considered as a promising alternative for treating infections caused by multi-drug resistant E. Read More

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http://dx.doi.org/10.1007/s12250-020-00233-2DOI Listing

Reagents for detection of Rift Valley fever virus infection in sheep.

J Vet Diagn Invest 2020 May 25:1040638720926476. Epub 2020 May 25.

CSIRO-Australian Animal Health Laboratory, East Geelong, Victoria, Australia.

Rift Valley fever virus (RVFV) causes Rift Valley fever (RVF), resulting in morbidity and mortality in humans and ruminants. Evidence of transboundary outbreaks means that RVFV remains a threat to human health and livestock industries in countries that are free from the disease. To enhance surveillance capability, methods for detection of RVFV are required. Read More

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http://dx.doi.org/10.1177/1040638720926476DOI Listing

Urinary tract infections in children.

Lancet 2020 05;395(10237):1659-1668

Children's Hospital of Pittsburgh, Pittsburgh, PA, USA.

Urinary tract infections (UTIs) in children are among the most common bacterial infections in childhood. They are equally common in boys and girls during the first year of life and become more common in girls after the first year of life. Dividing UTIs into three categories; febrile upper UTI (acute pyelonephritis), lower UTI (cystitis), and asymptomatic bacteriuria, is useful for numerous reasons, mainly because it helps to understand the pathophysiology of the infection. Read More

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http://dx.doi.org/10.1016/S0140-6736(20)30676-0DOI Listing

The Use of Bacteriophages in the Poultry Industry.

Animals (Basel) 2020 May 18;10(5). Epub 2020 May 18.

Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c St., 02-776 Warsaw, Poland.

The emergence of multidrug-resistant infections and antibiotic failures have raised concerns over human and veterinary medicine worldwide. Poultry production has had to confront the problems of an alarming increase in bacterial resistance, including zoonotic pathogens. According to the European Food Safety Authority (EFSA), campylobacteriosis and salmonellosis have been the most frequently reported human foodborne diseases linked to poultry. Read More

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http://dx.doi.org/10.3390/ani10050872DOI Listing

Phenotypic and genotypic quinolone resistance in Escherichia coli underlining GyrA83/87 mutations as a target to detect ciprofloxacin resistance.

J Antimicrob Chemother 2020 May 22. Epub 2020 May 22.

Université de Reims-Champagne-Ardenne, SFR CAP-Santé, Inserm UMR-S 1250 P3Cell, 51097, Reims, France.

Background: Quinolone resistance (QR) is one component of the MDR emerging in Escherichia coli and is of particular concern given the widespread use of fluoroquinolones.

Objectives: To characterize the QR phenotypes and genotypes in E. coli responsible for bloodstream infections and to propose molecular determinants that could be targeted to predict ciprofloxacin resistance. Read More

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http://dx.doi.org/10.1093/jac/dkaa189DOI Listing

Antibacterial-coated silk surgical sutures by ex situ deposition of silver nanoparticles synthesized with Eucalyptus camaldulensis eradicates infections.

J Microbiol Methods 2020 May 20;174:105955. Epub 2020 May 20.

Excellence Research Laboratory on Natural Products, Department of Microbiology, Faculty of Science and Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand. Electronic address:

Surgical site infection arising from microbial contamination of surgical wounds is a major cause of surgical complications and prolong hospital stay. In this study, silver nanoparticles (AgNPs) biosynthesized using Eucalyptus camaldulensis extract were deposited on silk surgical sutures by ex situ method. Adherence of AgNPs to the surface of sutures was observed, with significantly reduced surface roughness (323. Read More

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http://dx.doi.org/10.1016/j.mimet.2020.105955DOI Listing

Prevalence of Extended-Spectrum Beta-Lactamase-Producing Causing Bloodstream Infections in Cancer Patients from Southwest of Iran.

Infect Drug Resist 2020 6;13:1319-1326. Epub 2020 May 6.

Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Introduction: This study aimed to evaluate the frequency rate of extended-spectrum beta-lactamase-producing (ESBL-PE) causing bloodstream infections (BSIs) in cancer patients referred to one of the major referral hospitals in Ahvaz city, southwest Iran.

Materials And Methods: In this study, 1700 blood cultures were collected from 610 cancer patients suspected to have BSI from October 2016 to August 2017 referred to the Shafa cancer hospital, Ahvaz, southwest of Iran. The blood culture bottles were incubated aerobically at 35-37ºC for 24 hours and then sub-cultured on routine microbiology culture media. Read More

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http://dx.doi.org/10.2147/IDR.S254357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212769PMC

Topical Estrogen Treatment Augments the Vaginal Response to Escherichia coli Flagellin.

Sci Rep 2020 May 21;10(1):8473. Epub 2020 May 21.

Institute for Cell & Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK.

The female climacteric or menopausal process characterised by reduced estrogen, associates with an increased risk of recurrent urinary tract infections (rUTIs) linked to uropathogenic Escherichia coli (UPEC). Clinically, topical vaginal estrogen treatment has a prophylactic effect against such infections. The aim of this study was to investigate, in vitro, the effects of a topical estrogen treatment on vaginal epithelial responses following challenge with E. Read More

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http://dx.doi.org/10.1038/s41598-020-64291-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242342PMC

Complete Genome Sequence of Escherichia coli GW-AmxH19, Isolated from Hospital Wastewater in Greifswald, Germany.

Microbiol Resour Announc 2020 May 21;9(21). Epub 2020 May 21.

Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August University of Göttingen, Göttingen, Germany

The Gram-negative and rod-shaped strain GW-AmxH19 was isolated from university hospital wastewater in Greifswald, Germany. The genome consists of two replicons, including one circular chromosome (5.04 Mb) and a circular plasmid (126. Read More

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http://dx.doi.org/10.1128/MRA.00279-20DOI Listing

whISOBAX Inhibits Bacterial Pathogenesis and Enhances the Effect of Antibiotics.

Antibiotics (Basel) 2020 May 19;9(5). Epub 2020 May 19.

Department of Chemistry and Food Science, Framingham State University, Framingham, MA 01701, USA.

As bacteria are becoming more resistant to commonly used antibiotics, alternative therapies are being sought. whISOBAX (WH) is a witch hazel extract that is highly stable (tested up to 2 months in 37 °C) and contains a high phenolic content, where 75% of it is hamamelitannin and traces of gallic acid. Phenolic compounds like gallic acid are known to inhibit bacterial growth, while hamamelitannin is known to inhibit staphylococcal pathogenesis (biofilm formation and toxin production). Read More

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http://dx.doi.org/10.3390/antibiotics9050264DOI Listing

Microbiological profile of infections of the hip joint: An Indian perspective.

Indian J Med Microbiol 2019 Oct-Dec;37(4):509-513

Department of Orthopaedics, Christian Medical College, Vellore, Tamil Nadu, India.

Background: Knowledge of the local microbiological epidemiology helps in formulating protocols for appropriate treatment of hip infections. The aim of this study was to profile the organisms cultured from infected hips.

Methods: The microbiological profile and sensitivity pattern of organisms in eighty infected hips were reviewed. Read More

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http://dx.doi.org/10.4103/ijmm.IJMM_20_31DOI Listing

Comparative evaluation of microscan walkaway 96 plus ID/AST system and mikrolatest broth microdilution kit in assessing colistin susceptibility of carbapenem-resistant clinical gram-negative bacterial isolates: Experience from a tertiary care teaching hospital in Rishikesh, Uttarakhand.

Indian J Med Microbiol 2019 Oct-Dec;37(4):502-508

Department of Microbiology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India, IndiaIndia.

Context: As reports on colistin resistance are slowly emerging from different parts of the world, it is imperative that the clinical microbiology laboratories should generate accurate in vitro colistin susceptibility results.

Aim: The aim is to generate preliminary data on the diagnostic utility of MicroScan WalkAway 96 Plus Identification ID/ Antimicrobial susceptibility testing AST system in determining in vitro colistin susceptibility of carbapenem-resistant clinical Gram-negative bacterial isolates.

Settings And Design: A pilot study was conducted in a tertiary care teaching hospital located in Rishikesh, Uttarakhand, between May and June 2019. Read More

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http://dx.doi.org/10.4103/ijmm.IJMM_19_437DOI Listing

Invasive Infections in <3-Month-Old Infants in France: Clinical and Laboratory Features.

Front Pediatr 2020 6;8:204. Epub 2020 May 6.

Université de Paris, IAME, INSERM, Paris, France.

Few data are available on invasive group A (GAS) infections (IGASIs) in infants. We described initial clinical and laboratory features and outcomes of <3-month-old infants hospitalized for an IGASI between 2007 and 2016 in France. Patients were identified from the French National Reference Centre for streptococci. Read More

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http://dx.doi.org/10.3389/fped.2020.00204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217982PMC

Treatment Strategies for Infections With Shiga Toxin-Producing .

Front Cell Infect Microbiol 2020 6;10:169. Epub 2020 May 6.

Institute for Infectiology, University of Münster, Münster, Germany.

Infections with Shiga toxin-producing (STEC) cause outbreaks of severe diarrheal disease in children and the elderly around the world. The severe complications associated with toxin production and release range from bloody diarrhea and hemorrhagic colitis to hemolytic-uremic syndrome, kidney failure, and neurological issues. As the use of antibiotics for treatment of the infection has long been controversial due to reports that antibiotics may increase the production of Shiga toxin, the recommended therapy today is mainly supportive. Read More

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http://dx.doi.org/10.3389/fcimb.2020.00169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218068PMC

TRPV4 Mediates Acute Bladder Responses to Bacterial Lipopolysaccharides.

Front Immunol 2020 6;11:799. Epub 2020 May 6.

Laboratory for Ion Channel Research, Department of Cellular and Molecular Medicine, VIB Center for Brain & Disease Research, Leuven, Belgium.

Urinary tract infections (UTI) affect a large proportion of the population, causing among other symptoms, more frequent and urgent micturition. Previous studies reported that the gram-negative bacterial wall component lipopolysaccharides (LPS) trigger acute epithelial and bladder voiding responses, but the underlying mechanisms remain unknown. The cation channel TRPV4 is implicated in the regulation of the bladder voiding. Read More

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http://dx.doi.org/10.3389/fimmu.2020.00799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218059PMC

Rough-type and loss of the LPS due to lpx genes deletions are associated with colistin resistance in multidrug-resistant clinical Escherichia coli isolates not harbouring mcr genes.

PLoS One 2020 20;15(5):e0233518. Epub 2020 May 20.

Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

The emergence of multidrug-resistant Escherichia coli has become a great challenge in treating nosocomial infections. The polymyxin antibiotic colistin is used as a 'last-line' therapy for such strains, but resistance to colistin is increasingly emerging all over the world. In this study, we investigated lipopolysaccharides (LPS) of colistin-resistant isolates and examined mutations in lpx genes in strains not harbouring mcr genes. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233518PLOS
May 2020
3.234 Impact Factor