368,815 results match your criteria Escherichia Coli Infections


Metabolic Fingerprinting of Bacteria Exposed to Nanomaterials, Using Online Databases, NMR, and High-Resolution Mass Spectrometry.

Methods Mol Biol 2019 ;1894:271-280

Department of Chemistry, University of Ioannina, Ioannina, Greece.

Nanomaterials are examined more and more for their antibacterial properties. Herein, we propose a method for assessing the bactericidal properties of nanomaterials against Escherichia coli and Staphylococcus aureus, as well as a method to investigate the metabolic alterations occurring to bacteria, induced by their exposure to nanomaterials. Bacterial metabolome is extracted and metabolic fingerprint is recorded by H-NMR. Read More

View Article
January 2019

Chemical transformation mediated CRISPR/Cas9 genome editing in Escherichia coli.

Biotechnol Lett 2018 Dec 13. Epub 2018 Dec 13.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang, People's Republic of China.

Objectives: To develop a convenient chemical transformation mediated CRISPR/Cas9 (CT-CRISPR/Cas9) system for genome editing in Escherichia coli.

Results: Here, we have constructed a CT-CRISPR/Cas9 system, which can precisely edit bacterial genome (replacing, deleting, inserting or point mutating a target gene) through chemical transformation. Compared with the traditional electroporation mediated CRISPR/Cas9 (ET-CRISPR/Cas9) system, genome editing with the CT-CRISPR/Cas9 system is much cheaper and simpler. Read More

View Article
December 2018

Polyphenol-associated oxidative stress and inflammation in a model of LPS-induced inflammation in glial cells: do we know enough for responsible compounding?

Inflammopharmacology 2018 Dec 13. Epub 2018 Dec 13.

Department Physiological Sciences, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch, 7602, South Africa.

Cyanidin and chlorogenic acid are polyphenols from plant origin that are present in many common fruits, particularly in berries. To corroborate the protective or detrimental effects of both compounds from a neuro-inflammatory perspective, in vitro experiments were carried out in human astrocytes (U-373). Astrocytes were pre-treated with a range of concentrations of either cyanidin, chlorogenic acid or a combined treatment for a period of 30 min, before exposure to Escherichia coli lipopolysaccharide (LPS) challenge for 23. Read More

View Article
December 2018

Dynamics of Biofilm Formation by Salmonella Typhimurium and Beef Processing Plant Bacteria in Mono- and Dual-Species Cultures.

Microb Ecol 2018 Dec 13. Epub 2018 Dec 13.

Agriculture and Agri-Food Canada Lacombe Research and Development Centre, 6000 C & E Trail, Lacombe, Alberta, T4L 1W1, Canada.

This study aimed to determine the impact of bacteria from a beef plant conveyor belt on the biofilm formation of Salmonella in dual-species cultures. Beef plant isolates (50) including 18 Gram-negative aerobes (GNA), 8 Gram-positive aerobes (GPA), 5 lactic acid bacteria (LAB), 9 Enterobacteriaceae (EB), and 10 generic Escherichia coli (GEC) were included for developing biofilms in mono- and co-culture with S. Typhimurium at 15 °C for 6 days. Read More

View Article
December 2018

Risk Factors for Short- and Long-Term Outcomes in Children With STEC-HUS/D HUS: A Single-Center Experience.

Glob Pediatr Health 2018 4;5:2333794X18816920. Epub 2018 Dec 4.

The Children's Mercy Hospitals and Clinics, Kansas City, MO, USA.

. Hemolytic uremic syndrome (HUS) is one of the common causes for acute kidney injury in childhood. . Read More

View Article
December 2018

Anaerobic Degradation of the Plant Sugar Sulfoquinovose Concomitant With HS Production: K-12 and sp. Strain DF1 as Co-culture Model.

Front Microbiol 2018 27;9:2792. Epub 2018 Nov 27.

Department of Biology, University of Konstanz, Konstanz, Germany.

Sulfoquinovose (SQ, 6-deoxy-6-sulfoglucose) is produced by plants and other phototrophs and its biodegradation is a relevant component of the biogeochemical carbon and sulfur cycles. SQ is known to be degraded by aerobic bacterial consortia in two tiers via C-organosulfonates as transient intermediates to CO, water and sulfate. In this study, we present a first laboratory model for anaerobic degradation of SQ by bacterial consortia in two tiers to acetate and hydrogen sulfide (HS). Read More

View Article
November 2018

Epidemiology of bloodstream infections in patients with chronic lymphocytic leukemia: a longitudinal nation-wide cohort study.

Leukemia 2018 Dec 13. Epub 2018 Dec 13.

Department of Haematology, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark.

Patients with chronic lymphocytic leukemia (CLL) have a high risk of bloodstream infections (BSI). BSI cause significant morbidity and mortality among CLL patients; approximately one-third of fatalities in CLL list infections as cause of death. All CLL patients in Denmark diagnosed between 2008 and 2016 were followed through registries for the event of a BSI. Read More

View Article
December 2018

An automated bacterial concentration and recovery system for pre-enrichment required in rapid Escherichia coli detection.

Sci Rep 2018 Dec 13;8(1):17808. Epub 2018 Dec 13.

Jiangsu Delin Environmental Protection Technology Co., Ltd., Jiangsu, China.

One of the biggest challenges in rapid low concentration bacterial detection is the pre-concentration or pre-enrichment, which aims to increase bacteria concentration and reduce sample volume for easy bacterial detection. In practical bacterial detection, large-volume water samples with a pathogenic bacterial concentration of less than 1 CFU/mL have to be tested rapidly. The reported biosensors either have insufficient detection limit or have limited capability of handling a sufficiently large water sample. Read More

View Article
December 2018

.

J Biol Chem 2018 Dec 13. Epub 2018 Dec 13.

Biozentrum, University of Basel, Switzerland.

Substitution of protium (H) for deuterium (D) strongly affects biological systems. Whereas higher eukaryotes such as plants and mammals hardly survive a deuterium content of > 30%, many microorganisms can grow on fully deuterated media, albeit at reduced rates. Very little is known about how the H/D replacement influences life at the systems level. Read More

View Article
December 2018

An EmrB multidrug efflux pump in with unexpected roles in antibiotic resistance.

J Biol Chem 2018 Dec 13. Epub 2018 Dec 13.

Biological Sciences, Louisiana State University, United States.

The antibiotic trimethoprim is frequently used to manage infections, and members of the resistance-nodulation-division (RND) family of efflux pumps have been implicated in multidrug resistance of this species complex. We show here that a member of the distinct multidrug resistance B (EmrB) family is a primary exporter of trimethoprim in , as evidenced by increased trimethoprim sensitivity after inactivation of , the gene that encodes EmrB. We also found that the gene is up-regulated following addition of gentamicin and that this up-regulation is due to repression of the gene encoding OstR, a member of the multiple antibiotic resistance regulator (MarR) family. Read More

View Article
December 2018

Corrigendum to "Prophage phiv142-3 enhances the colonization and resistance to environmental stresses of avian pathogenic Escherichia coli" [Vet. Microbiol. 218 (2018) 70-77].

Vet Microbiol 2018 Dec 10. Epub 2018 Dec 10.

MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, China. Electronic address:

View Article
December 2018

α-Chymotrypsin Immobilized on a Low-Density Polyethylene Surface Successfully Weakens Biofilm Formation.

Int J Mol Sci 2018 Dec 12;19(12). Epub 2018 Dec 12.

Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, Milano 20133, Italy.

The protease α-chymotrypsin (α-CT) was covalently immobilized on a low-density polyethylene (LDPE) surface, providing a new non-leaching material (LDPE-α-CT) able to preserve surfaces from biofilm growth over a long working timescale. The immobilized enzyme showed a transesterification activity of 1.24 nmol/h, confirming that the immobilization protocol did not negatively affect α-CT activity. Read More

View Article
December 2018

Synthesis, Antibacterial, and Anti HepG2 Cell Line Human Hepatocyte Carcinoma Activity of Some New Potentially Benzimidazole-5-(Aryldiazenyl)Thiazole Derivatives.

Molecules 2018 Dec 11;23(12). Epub 2018 Dec 11.

Department of Biochemistry, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.

The paper describes the synthesis and biological evaluation of some new benzimidazole derivatives as potent clinical drugs that are useful in the treatment of some microbial infections and tumor inhibition. The starting compound 2-(bromomethyl)-1-benzimidazole () was prepared, and hence underwent interesting functionalization reactions to afford several series of benzimidazole-5-(aryldiazenyl)thiazole derivatives: ⁻, ⁻, and ⁻. The antibacterial activities of the synthesized compounds were evaluated by calculation of the inhibition zone diameter (mm) and the determination of minimum inhibitory concentration (µg/mL) against selected pathogenic bacteria (Gram-positive bacteria) and (Gram-negative bacteria). Read More

View Article
December 2018

High-Throughput Screen Identifying the Thiosemicarbazone NSC319726 Compound as a Potent Antimicrobial Lead Against Resistant Strains of Escherichia coli.

Biomolecules 2018 Dec 7;8(4). Epub 2018 Dec 7.

Zoetis Global Therapeutics Research, 333 Portage Street, Kalamazoo, 49007 MI, USA.

Antibiotic discovery is vital when considering the increasing antimicrobial resistance threat. The aim of this work was to provide a high-throughput screen (HTS) assay using multidrug-resistant strains to enable further research into antimicrobial lead discovery and identify novel antimicrobials. This study describes a primary HTS of a diverse library of 7884 small molecules against a susceptible strain. Read More

View Article
December 2018

Effects of Rich-Polyphenols Extract of Dendrobium loddigesii on Anti-Diabetic, Anti-Inflammatory, Anti-Oxidant, and Gut Microbiota Modulation in db/db Mice.

Molecules 2018 Dec 7;23(12). Epub 2018 Dec 7.

School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.

is a traditional Chinese herb with anti-diabetic effects and has diverse bibenzyls as well as phenanthrenes. Little is known about polyphenols anti-diabetic activities, so, a rich-polyphenols extract of (DJP) was used for treatment of diabetic db/db mice; the serum biochemical index and tissue appearance were evaluated. In order to gain an insight into the anti-diabetic mechanism, the oxidative stress index, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and gut microbiota modulation were determined by ELISA, immunohistochemistry or high throughput sequencing 16S rRNA gene. Read More

View Article
December 2018

Detailed Mode of Action of Arabinan-Debranching α--Arabinofuranosidase GH51 from .

J Microbiol Biotechnol 2018 Dec 7. Epub 2018 Dec 7.

Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Republic of Korea.

The gene encoding an α--arabinofuranosidase (BvAF) GH51 from FZB42 was cloned and expressed in . The corresponding open reading frame consists of 1,491 nucleotides which encodes 496 amino acids with the molecular mass of 56.9 kDa. Read More

View Article
December 2018

Isolation and characterization of an eosinophilic GH 16 β-agarase (AgaDL6) from an agar-degrading marine bacterium sp. HQM9.

J Microbiol Biotechnol 2018 Dec 7. Epub 2018 Dec 7.

College of Marine Science, Shandong University, Weihai 264209, China; Xiaoxu Tian, Chao Peng, National Facility for Protein Science in Shanghai, Zhangjiang Lab, Shanghai, 201210, China.

A special eosinophilic agarase exo-type β-agarase gene, , was cloned from a marine agar-degrading bacterium, sp. HQM9. The gene comprised 1383-bp nucleotides encoding a putative agarase AgaDL6 of 461 amino acids with a calculated molecular mass of 52. Read More

View Article
December 2018

The application of antibiotics in broiler production and the resulting antibiotic resistance in Escherichia coli: A global overview.

Poult Sci 2018 Dec 13. Epub 2018 Dec 13.

Department of Food Science and Technology, Institute of Food Science, BOKU-University of Natural Resources and Life Sciences, 1190 Vienna, Austria.

The increase in antibiotic resistance is a global concern for human and animal health. Resistant microorganisms can spread between food-producing animals and humans. The objective of this review was to identify the type and amount of antibiotics used in poultry production and the level of antibiotic resistance in Escherichia coli isolated from broilers. Read More

View Article
December 2018
1 Read

A role for 3' exonucleases at the final stages of chromosome duplication in Escherichia coli.

Nucleic Acids Res 2018 Dec 14. Epub 2018 Dec 14.

Division of Biosciences, College of Health and Life Sciences, Brunel University London, Uxbridge UB8 3PH, UK.

Chromosome duplication initiates via the assembly of replication fork complexes at defined origins, from where they proceed in opposite directions until they fuse with a converging fork. Recent work highlights that the completion of DNA replication is highly complex in both pro- and eukaryotic cells. In this study we have investigated how 3' and 5' exonucleases contribute towards the successful termination of chromosome duplication in Escherichia coli. Read More

View Article
December 2018

Evaluation of temocillin for phenotypic carbapenemase screening of Escherichia coli and Salmonella enterica isolates in relation to the presence of genes encoding ESBLs and carbapenemase production.

J Antimicrob Chemother 2018 Dec 13. Epub 2018 Dec 13.

Statens Serum Institut, Copenhagen, Denmark.

Background: The expression of enzymes of the OXA-48 carbapenemase group is difficult to detect by phenotypic methods owing to frequent low levels of carbapenem resistance and negative results with some screening methods. Temocillin has been shown to be a good option for phenotypic screening as it is hydrolysed by the OXA-48-group enzymes, whereas ESBLs, AmpC and some other carbapenemases have a lower hydrolytic effect on this antimicrobial. However, no epidemiological cut-off for temocillin is available. Read More

View Article
December 2018

Discovery of potent and selective inhibitors of the Escherichia coli M1-aminopeptidase via multicomponent solid-phase synthesis of tetrazole-peptidomimetics.

Eur J Med Chem 2018 Dec 1;163:481-499. Epub 2018 Dec 1.

Center for Natural Products Research, Faculty of Chemistry, University of Havana, Zapata y G, 10400, La Habana, Cuba; Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle/Saale, Germany. Electronic address:

The Escherichia coli neutral M1-aminopeptidase (ePepN) is a novel target identified for the development of antimicrobials. Here we describe a solid-phase multicomponent approach which enabled the discovery of potent ePepN inhibitors. The on-resin protocol, developed in the frame of the Distributed Drug Discovery (D3) program, comprises the implementation of parallel Ugi-azide four-component reactions with resin-bound amino acids, thus leading to the rapid preparation of a focused library of tetrazole-peptidomimetics (TPMs) suitable for biological screening. Read More

View Article
December 2018

Association between submerged aquatic vegetation and elevated levels of Escherichia coli and potential bacterial pathogens in freshwater lakes.

Sci Total Environ 2018 Dec 2;657:319-324. Epub 2018 Dec 2.

BioTechnology Institute, University of Minnesota, St. Paul, MN, USA; Department of Soil, Water, and Climate, University of Minnesota, St. Paul, MN, USA; Department of Plant, and Microbial Biology, University of Minnesota, St. Paul, MN, USA. Electronic address:

Fecal indicator bacteria such as Escherichia coli have been reported to persist and potentially grow in a wide variety of secondary habitats, such as water, beach sand, sediment, periphyton and some algae. However, little is known about their association with submerged macrophytes and how this may influence water quality. In this study, we examined the association of E. Read More

View Article
December 2018

Molecular characterization, expression and functional analysis of cystatin C in Japanese flounder (Paralichthys olivaceus).

Fish Shellfish Immunol 2018 Dec 10. Epub 2018 Dec 10.

Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, 266003, Qingdao, Shandong, China. Electronic address:

Cystatins are natural tight-binding reversible inhibitors of cysteine proteases found in a wide arrange of organisms. Studies have shown that cystatins play important roles under both physiological and pathological conditions in mammals. However, much less is known about fish cystatins. Read More

View Article
December 2018

Antibacterial Activity of Discaria americana Gillies ex Hook (Rhamnaceae).

J Ethnopharmacol 2018 Dec 10. Epub 2018 Dec 10.

Departamento de Química, Universidade Federal de Santa Maria,Santa Maria, RS, Brazil. Electronic address:

Ethnopharmacology Relevance: Discaria americana Gillies ex Hook (sin. Discaria febrifuga and Discaria longispina) (Rhamnaceae) is a plant native from Rio Grande do Sul (Southern Brazil), Uruguay and Argentine, and has been used in Brazilian traditional medicine as antipyretic agent, and for stomach disorders. In Rio Grande do Sul, Uruguay and Argentine, the roots, in decoction, are used as tonic and febrifuge. Read More

View Article
December 2018

Composite scaffold obtained by electro-hydrodynamic technique for infection prevention and treatment in bone repair.

Int J Pharm 2018 Dec 10. Epub 2018 Dec 10.

Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Rio Ebro-Edificio I+D, C/ Mariano Esquillor S/N, 50018 Zaragoza, Spain; Aragon Health Research Institute (IIS Aragon), 50009 Zaragoza, Spain. Electronic address:

Bone infection is a devastating condition resulting from implant or orthopaedic surgery. Therapeutic strategies are extremely complicated and may result in serious side effects or disabilities. The development of enhanced 3D scaffolds, able to promote efficient bone regeneration, combined with targeted antibiotic release to prevent bacterial colonization, is a promising tool for the successful repair of bone defects. Read More

View Article
December 2018

Use of a Water-Based Probiotic to Treat Common Gut Pathogens.

Int J Pharm 2018 Dec 10. Epub 2018 Dec 10.

UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London, WC1N 1AX, UK. Electronic address:

This work reports the anti-pathogenic effect of a commercially available water-based probiotic suspension, Symprove™, against three commonly encountered infectious organisms; Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA) and Shigella sonnei. An isothermal calorimetric assay was used to the monitor growth of the species individually and in binary combinations, while colony plate counting was used to enumerate viable cell numbers. It was observed that all pathogenic species were faster growing than the probiotic bacteria in Symprove™ after inoculation into growth medium yet in all instances bacterial enumeration at the end of the experiments revealed a significant reduction in the pathogen population compared with the controls. Read More

View Article
December 2018

A conformational switch from a closed apo- to an open holo-form equips the acyl carrier protein for acyl chain accommodation.

Biochim Biophys Acta Proteins Proteom 2018 Dec 10. Epub 2018 Dec 10.

Department of Biochemistry, University of Delhi South Campus, New Delhi 110021, India. Electronic address:

Acyl carrier proteins (ACPs) play crucial roles in the biosynthesis of fatty acids, non-ribosomal polypeptides and polyketides. The three-dimensional NMR structure of Leishmania major holo-LmACP, belonging to the type II pathway, has been reported previously, but the structure of its apo-form and its conformational differences with the holo-form remain to be explored. Here we report the crystal structures of apo-LmACP (wild-type and S37A mutant) at 2. Read More

View Article
December 2018

Host restriction of Escherichia coli recurrent urinary tract infection occurs in a bacterial strain-specific manner.

PLoS Pathog 2018 Dec 13;14(12):e1007457. Epub 2018 Dec 13.

Department of Molecular Microbiology and Center for Women's Infectious Disease Research, Washington University School of Medicine, St. Louis, Missouri, United States of America.

Urinary tract infections (UTI) are extremely common and can be highly recurrent, with 1-2% of women suffering from six or more recurrent episodes per year. The high incidence of recurrent UTI, including recurrent infections caused by the same bacterial strain that caused the first infection, suggests that at least some women do not mount a protective adaptive immune response to UTI. Here we observed in a mouse model of cystitis (bladder infection) that infection with two different clinical uropathogenic Escherichia coli (UPEC) isolates, UTI89 or CFT073, resulted in different kinetics of bacterial clearance and different susceptibility to same-strain recurrent infection. Read More

View Article
December 2018

Sensitivity and specificity of recombinant proteins in Toxocara spp. for serodiagnosis in humans: Differences in adult and child populations.

PLoS One 2018 13;13(12):e0208991. Epub 2018 Dec 13.

Department of Biotecnologia, Universidade Federal de Pelotas, RS, Brasil.

Toxocariasis is a neglected zoonosis that affects children and adults. Recombinant proteins have been widely investigated for diagnosis, achieving high sensitivity and specificity in an overall population; however, little is known about age as a factor in its application. This study aims to investigate the diagnostic potential of Toxocara canis TES-30 and TES-120 recombinant proteins in humans, differentiating between its performance in children and adults. Read More

View Article
December 2018

Binding of the bacterial adhesin FimH to its natural, multivalent high-mannose type glycan targets.

J Am Chem Soc 2018 Dec 13. Epub 2018 Dec 13.

Multivalent carbohydrate-lectin interactions at host-pathogen interfaces play a crucial role in the establishment of infections. Although competitive antagonists that prevent pathogen adhesion are promising anti-microbial drugs, the molecular mechanisms underlying these complex adhesion processes are still poorly understood. Here, we characterize the interactions between the fimbrial adhesin FimH from uropathogenic Escherichia coli strains and its natural high-mannose type N-glycan binding epitopes on uroepithelial glycoproteins. Read More

View Article
December 2018

Experiences in fosfomycin susceptibility testing and resistance mechanism determination in Escherichia coli from urinary tract infections in the UK.

J Med Microbiol 2018 Dec 13. Epub 2018 Dec 13.

3​Norwich Medical School, Norwich Research Park, Colney Lane, Norwich NR4 7TJ, UK.

Purpose: With an increase in the numbers of bacterial isolates resistant to first-line antibiotics, there has been a revival in the use of older drugs including fosfomycin with novel mechanisms of action. We aimed to investigate the prevalence and genotypic nature of fosfomycin resistance in Escherichia coli from urinary tract infections (UTIs) using the various methods available in the clinical microbiology laboratory.

Methodology: In total, 1000 culture-positive urine samples were assessed for the presence of E. Read More

View Article
December 2018

Investigation of the effect of β-lactam antibiotics and serum on growth and gene expression in Escherichia coli strain JJ1886

Turk J Med Sci 2018 12 12;48(6):1340-1350. Epub 2018 Dec 12.

Section of Basic and Industrial Microbiology, Department of Biology, Faculty of Science, İstanbul University, İstanbul, Turkey

Background/aim: β-Lactamase-producing Escherichia coli strain JJ1886 is an epidemic clone with high virulence properties. Because this strain can survive in the bloodstream, we aimed here to understand how β-lactam antibiotics and human serum affect the growth and gene expression of this bacterium.

Materials And Methods: We report the time-dependent growth effect of normal human serum and heat-inactivated serum, together with β-lactam antibiotics (including cefotaxime, ceftazidime, and carbenicillin), for E. Read More

View Article
December 2018

Antimicrobial Activity and Cytotoxicity of Ag(I) and Au(I) Pillarplexes.

Front Chem 2018 27;6:584. Epub 2018 Nov 27.

Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Oslo, Norway.

The biological activity of four pillarplex compounds featuring different metals and anions was investigated. The toxicity of the compounds against four bacterial strains [ (ATCC6633), (ATCC6538), (UVI isolate), ], one fungus (), and a human cell line (HepG2) was determined. Additionally, a UV-Vis titration study of the pillarplexes was carried out to check for stability depending on pH- and chloride concentration changes and evaluate the applicability in physiological media. Read More

View Article
November 2018

Antimicrobial resistance in bacterial pathogens among hospitalised patients with severe invasive infections.

Exp Ther Med 2018 Dec 14;16(6):4499-4510. Epub 2018 Sep 14.

Department of Clinical Pharmacy, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania.

The most severe infections are invasive infections, due to the fact that the germs can accumulate in multiple sites and produce a body-wide infection known as sepsis. Septic shock has the highest mortality rate among non-traumatic medical conditions. In this study, we aimed to evaluate the incidence and prevalence of invasive infections in a hospital environment. Read More

View Article
December 2018

Reversible senescence of human colon cancer cells after blockage of mitosis/cytokinesis caused by the CNF1 cyclomodulin from Escherichia coli.

Sci Rep 2018 Dec 12;8(1):17780. Epub 2018 Dec 12.

Department of Molecular Biology and The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, SE-90187, Umeå, Sweden.

Cytotoxic necrotizing factor 1 (CNF1), a protein toxin produced by extraintestinal pathogenic Escherichia coli, activates the Rho-family small GTPases in eukaryotic cell, thereby perturbing multiple cellular functions. Increasing epidemiological evidence suggests a link between CNF1 and human inflammatory bowel disease and colorectal cancer. At the cellular level, CNF1 has been hypothesized to reprogram cell fate towards survival due to the role in perturbing cell cycle and apoptosis. Read More

View Article
December 2018

Comparative transcriptome analysis revealed the improved β-carotene production in Sporidiobolus pararoseus yellow mutant MuY9.

J Gen Appl Microbiol 2018 Dec 13. Epub 2018 Dec 13.

College of Land and Environment, Shenyang Agricultural University.

Carotenoids are ubiquitous constituents of living organisms. These structurally diverse pigments have received considerable attention due to their biotechnological applications and potential beneficial effects on human health. In this study, we characterized an over-producing β-carotene mutant of Sporidiobolus pararoseus, obtained by ultraviolet mutagenesis, named MuY9. Read More

View Article
December 2018

HflX protein protects from manganese stress.

J Biosci 2018 Dec;43(5):1001-1013

Department of Biochemistry, Bose Institute, P-1/12, C.I.T. Scheme VIIM, Kolkata 700 054, India.

The ribosome-binding GTPase HflX is required for manganese homeostasis in . While under normal conditions ΔhflX cells behave like wild type with respect to growth pattern and morphology, deletion of makes cells extremely sensitive to manganese, characterized by arrested cell growth and filamentation. Here we demonstrate that upon complementation by , manganese stress is relieved. Read More

View Article
December 2018

Baseplate variability of chemoreceptor arrays.

Proc Natl Acad Sci U S A 2018 Dec 12. Epub 2018 Dec 12.

Institute of Biology, Leiden University, 2333 BE Leiden, The Netherlands;

The chemoreceptor array, a remarkably ordered supramolecular complex, is composed of hexagonally packed trimers of receptor dimers networked by a histidine kinase and one or more coupling proteins. Even though the receptor packing is universal among chemotactic bacteria and archaea, the array architecture has been extensively studied only in selected model organisms. Here, we show that even in the complete absence of the kinase, the cluster II arrays in retain their native spatial localization and the iconic hexagonal packing of the receptors with 12-nm spacing. Read More

View Article
December 2018

On mechanisms of colicin import: the outer membrane quandary.

Biochem J 2018 Dec 12;475(23):3903-3915. Epub 2018 Dec 12.

Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, U.S.A.

Current problems in the understanding of colicin import across the outer membrane (OM), involving a range of cytotoxic mechanisms, are discussed: (I) Crystal structure analysis of colicin E3 (RNAase) with bound OM vitamin B receptor, BtuB, and of the N-terminal translocation (T) domain of E3 and E9 (DNAase) inserted into the OM OmpF porin, provide details of the initial interaction of the colicin central receptor (R)- and N-terminal T-domain with OM receptors/translocators. (II) Features of the translocon include: (a) high-affinity ( ≈ 10 M) binding of the E3 receptor-binding R-domain E3 to BtuB; (b) insertion of disordered colicin N-terminal domain into the OmpF trimer; (c) binding of the N-terminus, documented for colicin E9, to the TolB protein on the periplasmic side of OmpF. Reinsertion of the colicin N-terminus into the second of the three pores in OmpF implies a colicin anchor site on the periplasmic side of OmpF. Read More

View Article
December 2018

Biomimetic recyclable microgels for on-demand generation of hydrogen peroxide and antipathogenic application.

Acta Biomater 2019 Jan 26;83:109-118. Epub 2018 Oct 26.

Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA. Electronic address:

Microgels that can generate antipathogenic levels of hydrogen peroxide (HO) through simple rehydration in solutions with physiological pH are described herein. HO is a widely used disinfectant but the oxidant is hazardous to store and transport. Catechol, an adhesive moiety found in mussel adhesive proteins, was incorporated into microgels, which generated 1-5 mM of HO for up to four days as catechol autoxidized. Read More

View Article
January 2019

Resistance profile of clinically relevant bacterial isolates against fluoroquinolone in Ethiopia: a systematic review and meta-analysis.

BMC Pharmacol Toxicol 2018 Dec 12;19(1):86. Epub 2018 Dec 12.

Department of Medical Laboratory Sciences, College of Health and Medical Sciences, Haramaya University, P.O.Box 235, Harar, Ethiopia.

Background: Fluoroquinolones are among the most frequently utilized antibacterial agents in developing countries like Ethiopia. Ciprofloxacin has become the most prescribed drug within this class and remains as one of the top three antibacterial agents prescribed in Ethiopia. However, several studies indicated that there is a gradual increase of antibacterial resistance. Read More

View Article
December 2018

Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis.

Virol J 2018 Dec 12;15(1):189. Epub 2018 Dec 12.

Laboratório de Imunologia Aplicada, Centro de Desenvolvimento Tecnológico - Núcleo de Biotecnologia, Pelotas, RS, Brazil.

Background: The avian infectious bronchitis virus (IBV) remains a significant source of loss in the poultry industry and early diagnosis is required to prevent the disease from spreading. This study examined the combined use of an ELISA and Western blot (WB) to detect antibodies against the nucleocapsid protein (N) of IBV. The coding sequence for N was amplified by RT-PCR and expressed in Escherichia coli. Read More

View Article
December 2018

Infectious complications following probiotic ingestion: a potentially underestimated problem? A systematic review of reports and case series.

BMC Complement Altern Med 2018 Dec 12;18(1):329. Epub 2018 Dec 12.

National Institute of Cardiology, Rio de Janeiro/RJ, Brazil.

Background: Little is studied about complications related to probiotic ingestion. This study proposes to present a synthesis and critical evaluation of the reports and series of cases on the infectious complications related to the ingestion of probiotics, which can raise awareness for the prescribing and use of probiotics for certain groups of patients.

Methods: Systematic review of reports and series of cases researched in the PubMed, SciELO and Scopus databases published until August 2018. Read More

View Article
December 2018

Occurrence and Antibiotic Resistance of Enterobacteriaceae in Acute Leukemia Patients.

Klin Onkol 2018 ;31(4):282-288

Background: Acute leukemia (AL) is a heterogeneous group of malignant hematopoietic diseases and is divided into two basic types: acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). Patients with these diseases are highly immunosuppressed and therefore at a high risk of serious infections. This study aimed to perform active surveillance of enterobacteria, which cause these infections, and to determine their antibiotic resistance in patients with AL who were hospitalized at the Hemato-Oncology Center of University Hospital Olomouc. Read More

View Article
January 2018

Study of class 1 integrons in multidrug-resistant uropathogenic isolated from different hospitals in Karachi.

Iran J Basic Med Sci 2018 Oct;21(10):1079-1082

Dada Bhoy Institute of Higher Education Commission, Karachi, Pakistan.

Objectives: is the key pathogen in the family producing ESBL (extended spectrum β-lactamase) and associated with community-acquired infections. Therefore, this study was planned to determine the antibiotic susceptibility pattern of uropathogenic , prevalence of the ESBL gene group and class 1 integrons.

Materials And Methods: Clinical isolates of uropathogenic were isolated from different hospitals of Karachi. Read More

View Article
October 2018

A decrease in anaerobic bacteria promotes overgrowth while β-glucan treatment restores the gut microbiota and attenuates colitis.

Gut Pathog 2018 3;10:50. Epub 2018 Dec 3.

1U995/Team2, INSERM, 59000 Lille, France.

Background: The intestinal microbiota plays a crucial role in the maintenance of gut homeostasis. Changes in crosstalk between the intestinal epithelial cells, immune cells and the microbiota are critically involved in the development of inflammatory bowel disease. In the experimental mouse model, the development of colitis induced by dextran sulfate sodium (DSS) promotes overgrowth of the opportunistic yeast pathogen . Read More

View Article
December 2018

Characterization of a New Cyclohexylamine Oxidase From sp. YT-02.

Front Microbiol 2018 22;9:2848. Epub 2018 Nov 22.

School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China.

Cyclohexylamine (CHAM) is widely used in various industries, but it is harmful to human beings and the environment. sp. YT-02 can degrade CHAM via cyclohexanone as an intermediate. Read More

View Article
November 2018

Clever Cooperation: Interactions Between EspF and Host Proteins.

Front Microbiol 2018 22;9:2831. Epub 2018 Nov 22.

Department of Microbiology, School of Public Health, Southern Medical University, Guangzhou, China.

EspF is a central effector protein of enterohemorrhagic (EHEC), enteropathogenic (EPEC), and (CR) that is secreted through the type III secretion system to host cells. The interaction between EspF and host proteins plays an important role in bacterial pathogenesis. EspF protein binds to host SNX9 and N-WASP proteins to promote the colonization of pathogenic bacteria in intestinal epithelial cells; combines with cytokeratin 18, actin, 14-3-3ζ, Arp2/3, profilin, and ZO-1 proteins to intervene in the redistribution of intermediate filaments, the rearrangement of actin, and the disruption of tight junctions; acts together with Abcf2 to boost host cell intrinsic apoptosis; and collaborates with Anxa6 protein to inhibit phagocytosis. Read More

View Article
November 2018

Improved Microbial Safety of Direct Ozone-Depurated Shellstock Eastern Oysters () by Superchilled Storage.

Front Microbiol 2018 22;9:2802. Epub 2018 Nov 22.

Doctorate Program in Agricultural Sciences, Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Veracruz, Mexico.

The effect of superchilled storage at -1°C on the microbial safety of oyster depurated with 0.2, 0.4, and 0. Read More

View Article
November 2018

A Metabolomics Approach Reveals Immunomodulatory Effects of Proteinaceous Molecules Derived From Gut Bacteria Over Human Peripheral Blood Mononuclear Cells.

Front Microbiol 2018 13;9:2701. Epub 2018 Nov 13.

Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Food Science, University of Vigo, Ourense, Spain.

There are strong evidences that probiotics influence the immune status of the host, in a strain-specific manner, acting in the gastrointestinal tract. On the hypothesis that certain extracellular proteins and peptides from gut bacteria may mediate part of this immunomodulation and assuming they are able to diffuse through the mucus layer and interact with immune cells we have developed this work. Our study attempts to understand the immunomodulatory mechanisms of (i) Pext, the extracellular protein fraction of DSM20079, (ii) HM14, a peptide encrypted in an extracellular glycoside hydrolase from NCIMB 8809 and (iii) O111:B4 lipopolysaccharide (LPS), a well-known pro-inflammatory molecule, over human peripheral blood mononuclear cells (PBMCs). Read More

View Article
November 2018