7,972 results match your criteria Erythroleukemia


Synthesis in Escherichia coli and Characterization of Human Recombinant Erythropoietin with Additional Heparin-Binding Domain.

Biochemistry (Mosc) 2018 Oct;83(10):1207-1221

Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, 123098, Russia.

Recombinant human erythropoietin (EPO) with additional N-terminal heparin-binding protein domain (HBD) from bone morphogenetic protein 2 was synthesized in Escherichia coli cells. A procedure for HBD-EPO purification and refolding was developed for obtaining highly-purified HBD-EPO. The structure of recombinant HBD-EPO was close to that of the native EPO protein. Read More

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October 2018

Imidazo[1,2-]pyrazole-7-carboxamides Induce Apoptosis in Human Leukemia Cells at Nanomolar Concentrations.

Molecules 2018 Nov 1;23(11). Epub 2018 Nov 1.

Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary.

Leukemia, the malignancy of the hematopoietic system accounts for 10% of cancer cases with poor overall survival rate in adults; therefore, there is a high unmet medical need for the development of novel therapeutics. Eight imidazo[1,2-]pyrazole-7-carboxamides have been tested for cytotoxic activity against five leukemia cell lines: Acute promyelocytic leukemia (HL-60), acute monocytic leukemia (THP-1), acute T-lymphoblastic leukemia (MOLT-4), biphenotypic B myelomonocytic leukemia (MV-4-11), and erythroleukemia (K-562) cells in vitro. Imidazo[1,2-]pyrazole-7-carboxamides hampered the viability of all five leukemia cell lines with different potential. Read More

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November 2018
5 Reads

MRTFA augments megakaryocyte maturation by enhancing the SRF regulatory axis.

Blood Adv 2018 Oct;2(20):2691-2703

Department of Cell Biology.

Serum response factor (SRF) is a ubiquitously expressed transcription factor that binds DNA at CArG (CC[A/T]GG) domains in association with myocardin-family proteins (eg, myocardin-related transcription factor A [MRTFA]) or the ternary complex factor family of E26 transformation-specific (ETS) proteins. In primary hematopoietic cells, knockout of either SRF or MRTFA decreases megakaryocyte (Mk) maturation causing thrombocytopenia. The human erythroleukemia (HEL) cell line mimics the effects of MRTFA on Mk maturation, and MRTFA overexpression (MRTFA) in HEL cells enhances megakaryopoiesis. Read More

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October 2018
10 Reads

Design, synthesis, and biological evaluation of novel nitric oxide releasing dehydroandrographolide derivatives.

Chin J Nat Med 2018 Oct;16(10):782-790

State Key Laboratory of Natural Medicines, Center of Drug Discovery, China Pharmaceutical University, Nanjing 210009, China; Jiangsu Key Laboratory of Drug Discovery for Metabolic Disease, China Pharmaceutical University, Nanjing 210009, China. Electronic address:

A series of new hybrids of dehydroandrographolide (TAD), a biologically active natural product, bearing nitric oxide (NO)-releasing moieties were synthesized and designated as NO-donor dehydroandrographolide. The biological activities of target compounds were studied in human erythroleukemia K562 cells and breast cancer MCF-7 cells. Biological evaluation indicated that the most active compound I-5 produced high levels of NO and inhibited the proliferation of K562 (IC 1. Read More

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October 2018
1 Read

CARM1-mediated methylation of protein arginine methyltransferase 5 represses human γ-globin gene expression in erythroleukemia cells.

J Biol Chem 2018 Nov 26;293(45):17454-17463. Epub 2018 Sep 26.

From the State Key Laboratory of Pharmaceutical Biotechnology, Department of Hematology, the Affiliated Drum Tower Hospital of Nanjing University Medical School, China-Australia Center for Translational Medicine, School of Life Sciences, Nanjing University, Nanjing 210023, China,

Protein arginine methyltransferase 5 (PRMT5) is a member of the arginine methyltransferase protein family that critically mediates the symmetric dimethylation of Arg-3 at histone H4 (H4R3me2s) and is involved in many key cellular processes, including hematopoiesis. However, the post-translational modifications (PTMs) of PRMT5 that may affect its biological functions remain less well-understood. In this study, using MS analyses, we found that PRMT5 itself is methylated in human erythroleukemia Lys-562 cells. Read More

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November 2018
6 Reads

A novel role for in primitive erythropoiesis.

Development 2018 Oct 11;145(19). Epub 2018 Oct 11.

Australian Centre for Blood Diseases, Monash University, Melbourne, VIC 3004, Australia

Stem cell leukemia ( or ) and lymphoblastic leukemia 1 () encode highly related members of the basic helix-loop-helix family of transcription factors that are co-expressed in the erythroid lineage. Previous studies have suggested that is essential for primitive erythropoiesis. However, analysis of single-cell RNA-seq data of early embryos showed that primitive erythroid cells express both and Therefore, to determine whether can function in primitive erythropoiesis, we crossed conditional knockout mice with mice expressing a Cre recombinase under the control of the Epo receptor, active in erythroid progenitors. Read More

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October 2018
1 Read
6.460 Impact Factor

Reduction of Thermotolerance by Heat Shock Protein 90 Inhibitors in Murine Erythroleukemia Cells.

Biol Pharm Bull 2018 ;41(9):1393-1400

Department of Chemistry, Faculty of Science, Fukuoka University.

Cells induce heat shock proteins (HSPs) against various stress. However, murine erythroleukemia (MEL) cells do not express HSP72, a heat-inducible member of HSP70 family. So, it is of interest to examine how MEL cells respond to heat stress (44°C, 30 min). Read More

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November 2018
1 Read

Knockdown of Long Noncoding RNA Plasmacytoma Variant Translocation 1 with Antisense Locked Nucleic Acid GapmeRs Exerts Tumor-Suppressive Functions in Human Acute Erythroleukemia Cells Through Downregulation of C-MYC Expression.

Cancer Biother Radiopharm 2018 Aug 24. Epub 2018 Aug 24.

Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences , Isfahan, Iran .

Objective: Acute erythroleukemia (AEL) is a subtype of acute myeloid leukemia (AML), with no specific treatment. Up- or downregulation of long noncoding RNAs (lncRNAs) is strongly associated with the formation and progression of many malignancies. Plasmacytoma variant translocation 1 (PVT1) is a significantly upregulated lncRNA in AML. Read More

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August 2018
14 Reads

Perylenequione Derivatives with Anticancer Activities Isolated from the Marine Sponge-Derived Fungus, sp. SCSIO41014.

Mar Drugs 2018 Aug 14;16(8). Epub 2018 Aug 14.

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.

Seven new secondary metabolites classified as two perylenequinone derivatives ( and ), an altenusin derivative (), two phthalide racemates ( and ), and two phenol derivatives ( and ), along with twenty-one known compounds (⁻) were isolated from cultures of the sponge-derived fungus, sp. SCSIO41014. The structures and absolute configurations of these new compounds (⁻) were determined by spectroscopic analysis, X-ray single crystal diffraction, chiral-phase HPLC separation, and comparison of ECD spectra to calculations. Read More

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August 2018
5 Reads

Interleukin 4 is inactivated via selective disulfide-bond reduction by extracellular thioredoxin.

Proc Natl Acad Sci U S A 2018 08 13;115(35):8781-8786. Epub 2018 Aug 13.

Department of Chemistry, Stanford University, Stanford, CA 94305;

Thioredoxin 1 (TRX), an essential intracellular redox regulator, is also secreted by mammalian cells. Recently, we showed that TRX activates extracellular transglutaminase 2 via reduction of an allosteric disulfide bond. In an effort to identify other extracellular substrates of TRX, macrophages derived from THP-1 cells were treated with NP161, a small-molecule inhibitor of secreted TRX. Read More

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August 2018
6 Reads

Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells.

Cytotechnology 2018 Dec 2;70(6):1509-1518. Epub 2018 Aug 2.

Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Science, Isfahan, 81744-176, Iran.

New achievements in the field of cancer treatment are results of recent advances in molecular medicine and gene therapy. Usage of microRNAs (miRNAs) which are small noncoding RNAs is one of the molecular research lines for the diagnosis and treatment of cancers. miRNAs have an important role in post-transcriptional regulation of the gene expression and are involved in cellular activities such as growth, differentiation, cell death and cancer development. Read More

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December 2018
8 Reads

Lam. Decreases Oxidative Stress in Blood Cells and Prevents Doxorubicin-Induced Cardiotoxicity.

Oxid Med Cell Longev 2018 28;2018:2935051. Epub 2018 Jun 28.

Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil.

Doxorubicin (DOX) is an efficient chemotherapeutic agent, but its clinical application is limited by its cardiotoxicity associated with increased oxidative stress. Thus, the combination of DOX and antioxidants has been encouraged. In this study, we evaluated (I) the chemical composition and antioxidant capacity of aqueous extracts from stem bark (GUEsb) and leaves (GUEl) in 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, 2,2'-azobis(2-amidinopropane) dihydrochloride- (AAPH-) or DOX-induced lipid peroxidation inhibition in human blood cells, and intracellular reactive oxygen species (ROS) quantification using the fluorescent probe dichloro-dihydro-fluorescein diacetate (DCFH-DA) in K562 erythroleukemia cells incubated with GUEsb and stimulated with hydrogen peroxide; (II) the viability of K562 cells and human leukocytes treated with GUEsb in the absence or presence of DOX using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; (III) the acute toxicity of GUEsb; and (IV) the cardioprotective effect of GUEsb in C57Bl/6 mice treated with DOX. Read More

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October 2018
3 Reads

Induction of apoptosis and necrosis in human acute erythroleukemia cells by inhibition of long non-coding RNA PVT1.

Mol Biol Res Commun 2018 Jun;7(2):89-96

Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Recent advances in molecular medicine have proposed new therapeutic strategies for cancer. One of the molecular research lines for the diagnosis and treatment of cancer is the use of long non-coding RNAs (LncRNAs) which are a class of non-coding RNA molecules longer than 200 base pairs in length that act as the key regulator of gene expression. Different aspects of cellular activities like cell growth, proliferation, differentiation, apoptosis and migration are regulated by lncRNAs. Read More

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June 2018
1 Read

Response to erythropoietin in pediatric patients with chronic kidney disease: insights from an in vitro bioassay.

Pediatr Nephrol 2018 Nov 20;33(11):2123-2129. Epub 2018 Jul 20.

Department of Pediatrics C, Schneider Children's Medical Center of Israel, Sackler Faculty of Medicine, Tel-Aviv University, 14 Kaplan St., Petah Tikva, Israel.

Background: Decreased production of erythropoietin (EPO) is a major cause of anemia associated with chronic kidney disease (CKD). Treatment with recombinant human EPO (rHuEPO) improves patients' quality of life and survival; however, there is a marked variability in response to rHuEPO. At present, no available laboratory test is capable of evaluating responsiveness to EPO treatment. Read More

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November 2018
2 Reads

Identification of a novel enhancer/chromatin opening element associated with high-level γ-globin gene expression.

Mol Cell Biol 2018 Jul 16. Epub 2018 Jul 16.

Department of Biochemistry and Molecular Biology, College of Medicine, Health Cancer Center, Genetics Institute, Center for Epigenetics, University of Florida, Gainesville, Florida, 32610, U.S.A.

The organization of the five β-type globin genes on chromosome 11 reflects the timing of expression during erythroid development, with the embryonic ε-globin gene located at the 5'end, followed by the two fetal γ-globin genes, and the adult β- and δ- globin genes at the 3'end. Here, we functionally characterized a DNase I hypersensitive site located 4 kb upstream of the Gγ-globin gene (HBG-4kb HS). This site is occupied by transcription factors USF1, USF2, EGR1, MafK, and NF-E2 in the human erythroleukemia cell line K562 and exhibits histone modifications typical for enhancers. Read More

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July 2018
17 Reads

Benzofuran hydrazones as potential scaffold in the development of multifunctional drugs: Synthesis and evaluation of antioxidant, photoprotective and antiproliferative activity.

Eur J Med Chem 2018 Aug 2;156:118-125. Epub 2018 Jul 2.

Department of Life and Environmental Sciences, Unit of Pharmaceutical, Pharmacological and Nutraceutical Sciences, University of Cagliari, Via Ospedale 72, Cagliari, I-09124, Italy. Electronic address:

New benzofuranhydrazones 3-12 were easily prepared and assayed for their radical-scavenging ability. Hydrazones 3-12 showed different extent antioxidant activity in DPPH, FRAP and ORAC assays. Good antioxidant activity is related to the number and position of hydroxyl groups on the arylidene moiety. Read More

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August 2018
2 Reads

A novel synthesized 3', 5'-diprenylated chalcone mediates the proliferation of human leukemia cells by regulating apoptosis and autophagy pathways.

Biomed Pharmacother 2018 Oct 11;106:794-804. Epub 2018 Jul 11.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province, Chinese Academy of Sciences, Guiyang, 550014, China. Electronic address:

Leukemia is a hematologic malignancy with poor prognosis in humans and chemotherapy is the main strategy for treating leukemia patients. Novel drugs with better selectivity and lower toxicity are required for the treatment of patients. A novel 3',5'-diprenylated chalcone, (E)-1-(2-hydroxy-4-methoxy-3,5-diprenyl) phenyl-3-(3- pyridinyl)-propene-1-one (C10) is a potential new anti-leukemia agent. Read More

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October 2018
16 Reads

C-phycocyanin to overcome the multidrug resistance phenotype in human erythroleukemias with or without interaction with ABC transporters.

Biomed Pharmacother 2018 Oct 11;106:532-542. Epub 2018 Jul 11.

Laboratório de Cultura Celular, ICB, FURG, RS, Brazil; Programa de Pós-Graduação em Ciências Fisiológicas, ICB, FURG, RS, Brazil.

The phenotype of multidrug resistance (MDR) is one of the main causes of chemotherapy failure. Our study investigated the effect of C-phycocyanin (C-PC) in three human erythroleukemia cell lines with or without the MDR phenotype: K562 (non-MDR; no overexpression of drug efflux proteins), K562-Lucena (MDR; overexpression of ATP-binding cassette, sub-family B/ABCB1), and FEPS (MDR; overexpression of ABCB1 and ATP-binding cassette, sub-family C/ABCC1). Using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, we showed that 20 and 200 μg/mL C-PC decreased K562 viable cells after 24 h and 200 μg/mL C-PC decreased K562-Lucena cell proliferation after 48 h. Read More

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October 2018

Pure erythroid leukemia in a polymyositis patient treated with azathioprine.

Rare Tumors 2018 14;10:2036361318773847. Epub 2018 May 14.

Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Kagawa University, Miki, Japan.

Acute erythroid leukemia, also known as acute myeloid leukemia-M6, may be associated with previous chemotherapy or immunosuppressive therapy. For 10 years, a 69-year-old Japanese female patient with pure erythroid leukemia (or acute myeloid leukemia-M6b) was treated for polymyositis with 50-100 mg/day azathioprine. She complained of dyspnea with low-grade fever and was diagnosed as having pure erythroid leukemia. Read More

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May 2018
2 Reads

Selective dissociation between LSD1 and GFI1B by a LSD1 inhibitor NCD38 induces the activation of super-enhancer in erythroleukemia cells.

Oncotarget 2018 Apr 20;9(30):21007-21021. Epub 2018 Apr 20.

Department of Medicine, Shiga University of Medical Science, Otsu, Shiga, Japan.

Lysine-specific demethylase 1 (LSD1) is a histone modifier for transcriptional repression involved in the regulation of hematopoiesis. We previously reported that a LSD1 inhibitor NCD38 induces transdifferentiation from erythroid lineage to granulomonocytic lineage and exerts anti-leukemia effect through de-repression of the specific super-enhancers of hematopoietic regulators including in a human erythroleukemia cell line, HEL. However, the mechanistic basis for this specificity of NCD38 has remained unclear. Read More

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April 2018
8 Reads

Shifting of erythroleukemia to myelodysplastic syndrome according to the revised WHO classification: Biologic and cytogenetic features of shifted erythroleukemia.

Leuk Res 2018 Apr 30;70:13-19. Epub 2018 Apr 30.

Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea. Electronic address:

The 2016 revision of the World Health Organization (WHO) classification of tumours of haematopoietic and lymphoid tissues was published. According to 2016 WHO criteria, diagnostic criteria of acute erythroid leukemia was revised. We reassessed 34 de novo acute erythroid leukemia (AEL) diagnosed by 2008 WHO criteria, according to 2016 WHO criteria. Read More

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April 2018
4 Reads

Psychoneuroimmunology and Natural Killer Cells: The Chromium-Release Whole-Blood Assay.

Methods Mol Biol 2018 ;1781:209-220

Institute for Neuro-Immune Medicine, Nova Southeastern University, Ft. Lauderdale, FL, USA.

Natural killer (NK) cells are an essential component of innate immunity. These lymphocytes are also sensitive barometers of the effects of endogenous and exogenous stressors on the immune system. This chapter describes a chromium (Cr)-release bioassay designed to measure to the target cell killing capacity of NK cells (NKCC). Read More

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January 2018
2 Reads

Modulating the expression of Chtop, a versatile regulator of gene-specific transcription and mRNA export.

RNA Biol 2018 11;15(7):849-855. Epub 2018 May 11.

a Department of Applied Biological Science , United Graduate School of Agriculture, Tokyo University of Agriculture and Technology , Fuchu , Tokyo , Japan.

Chtop binds competitively to the arginine methyltransferases PRMT1 and PRMT5, thereby promoting the asymmetric or symmetric methylation of arginine residues, respectively. In cooperation with PRMT1, Chtop activates transcription of certain gene groups, such as the estrogen-inducible genes in breast cancer cells, the 5-hydroxymethylcytosine-modified genes involved in glioblastomagenesis, or the Zbp-89-dependent genes in erythroleukemia cells. Chtop also represses expression of the fetal γ-globin gene. Read More

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May 2018
12 Reads

Defective RAB1B-related megakaryocytic ER-to-Golgi transport in RUNX1 haplodeficiency: impact on von Willebrand factor.

Blood Adv 2018 04;2(7):797-806

Sol Sherry Thrombosis Research Center and.

Patients with RUNX1 haplodeficiency have thrombocytopenia, platelet dysfunction, and deficiencies of α-granules and dense granules. Platelet expression profiling of a patient with a heterozygous mutation (c.969-323G>T) revealed decreased , which encodes a small G protein. Read More

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April 2018
2 Reads

Drug-DNA adducts as biomarkers for metabolic activation of the nitro-aromatic nitrogen mustard prodrug PR-104A.

Biochem Pharmacol 2018 Aug 7;154:64-74. Epub 2018 Apr 7.

Auckland Cancer Society Research Centre, The University of Auckland, Auckland, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland, New Zealand.

PR-104A is a clinical-stage nitrogen mustard prodrug that is activated for DNA alkylation by reduction of a nitro group to the corresponding hydroxylamine (PR-104H) or amine (PR-104M). Metabolic reduction is catalysed by flavoreductases such as cytochrome P450 oxidoreductase (POR) under hypoxia, or by aldo-ketoreductase 1C3 (AKR1C3) independently of hypoxia. The unstable reduced metabolites are challenging to measure in biological samples, and biomarkers of the metabolic activation of PR-104A have not been used in the clinical evaluation of PR-104 to date. Read More

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August 2018
7 Reads

Molecular evidence of JAK2 p.V617F mutated pure erythroid leukemia arising from polycythemia vera.

Virchows Arch 2018 07 2;473(1):131-135. Epub 2018 Apr 2.

Department of Pathology, The Johns Hopkins University School of Medicine, 600 N. Wolfe St., Pathology Building, Room 401, Baltimore, MD, 21287, USA.

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July 2018
4 Reads

NF90/ILF3 is a transcription factor that promotes proliferation over differentiation by hierarchical regulation in K562 erythroleukemia cells.

PLoS One 2018 28;13(3):e0193126. Epub 2018 Mar 28.

Pulmonary and Critical Care Medicine, Stanford University School of Medicine, Stanford, California, United States of America.

NF90 and splice variant NF110 are DNA- and RNA-binding proteins encoded by the Interleukin enhancer-binding factor 3 (ILF3) gene that have been established to regulate RNA splicing, stabilization and export. The roles of NF90 and NF110 in regulating transcription as chromatin-interacting proteins have not been comprehensively characterized. Here, chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) identified 9,081 genomic sites specifically occupied by NF90/NF110 in K562 cells. Read More

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June 2018
6 Reads

High-level embryonic globin production with efficient erythroid differentiation from a K562 erythroleukemia cell line.

Exp Hematol 2018 06 7;62:7-16.e1. Epub 2018 Mar 7.

Sickle Cell Branch, National Heart Lung and Blood Institute/National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.

A reliable cell line capable of robust in vitro erythroid differentiation would be useful to investigate red blood cell (RBC) biology and genetic strategies for RBC diseases. K562 cells are widely utilized for erythroid differentiation; however, current differentiation methods are insufficient to analyze globin proteins. In this study, we sought to improve erythroid differentiation from K562 cells to enable protein-level globin analysis. Read More

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June 2018
6 Reads

ChIP-seq and ChIP-exo profiling of Pol II, H2A.Z, and H3K4me3 in human K562 cells.

Sci Data 2018 03 6;5:180030. Epub 2018 Mar 6.

Department of Molecular Physiology and Biophysics, Vanderbilt Genetics Institute, Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, TN 37232, USA.

The human K562 chronic myeloid leukemia cell line has long served as an experimental paradigm for functional genomic studies. To systematically and functionally annotate the human genome, the ENCODE consortium generated hundreds of functional genomic data sets, such as chromatin immunoprecipitation coupled to sequencing (ChIP-seq). While ChIP-seq analyses have provided tremendous insights into gene regulation, spatiotemporal insights were limited by a resolution of several hundred base pairs. Read More

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Riluzole Inhibits Proliferation, Migration and Cell Cycle Progression and Induces Apoptosis in Tumor Cells of Various Origins.

Anticancer Agents Med Chem 2018 ;18(4):565-572

Department of Medical Biology, Institute of Agricultural Medicine, 20-090 Lublin, Poland.

Background: Regardless of contemporary improvements in cancer treatment, the results of drug treatment are not always efficacious. Thus, the development of novel approaches that affect cancer cell-specific metabolic pathways is needed. Since much evidence has shown that tumor cell proliferation and motility are stimulated by glutamate via activation of its receptors, use of antagonists to these receptors may be the key to control cancer cell progression. Read More

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January 2018
17 Reads

Design and synthesis of new potent N,N-bis(arylalkyl)piperazine derivatives as multidrug resistance (MDR) reversing agents.

Eur J Med Chem 2018 Mar 5;147:7-20. Epub 2018 Feb 5.

Department of Neuroscience, Psychology, Drug Research and Child's Health - Section of Pharmaceutical and Nutraceutical Sciences, University of Florence, via Ugo Schiff 6, 50019 Sesto Fiorentino (FI), Italy. Electronic address:

A series of 1,4-substituted arylalkyl piperazine derivatives were synthesized and studied with the aim to obtain potent P-gp-dependent multidrug-resistant (MDR) reversers. The new compounds were designed on the basis of the structures of our previous arylamine ester derivatives endowed with high P-gp-dependent multidrug resistance reversing activity. All new compounds were active in the pirarubicin uptake assay on the doxorubicin-resistant erythroleukemia K562 cells (K562/DOX). Read More

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March 2018
25 Reads

Cooperation between Hsp90 and mortalin/GRP75 in resistance to cell death induced by complement C5b-9.

Cell Death Dis 2018 Feb 2;9(2):150. Epub 2018 Feb 2.

Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel.

Cancer cells are commonly more resistant to cell death activated by the membranolytic protein complex C5b-9. Several surface-expressed and intracellular proteins that protect cells from complement-dependent cytotoxicity (CDC) have been identified. In this study, we investigated the function of heat shock protein 90 (Hsp90), an essential and ubiquitously expressed chaperone, overexpressed in cancer cells, in C5b-9-induced cell death. Read More

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February 2018
8 Reads

Disulfide-masked iron prochelators: Effects on cell death, proliferation, and hemoglobin production.

J Inorg Biochem 2018 03 4;180:186-193. Epub 2018 Jan 4.

Department of Chemistry and Biochemistry, The University of Arizona, United States. Electronic address:

The iron metabolism of malignant cells, which is altered to ensure higher acquisition and utilization, motivates the investigation of iron chelation strategies in cancer treatment. In a prochelation approach aimed at increasing intracellular specificity, disulfide reduction/activation switches are incorporated on iron-binding scaffolds resulting in intracellularly activated scavengers. Herein, this strategy is applied to several tridentate donor sets including thiosemicarbazones, aroylhydrazones and semicarbazones. Read More

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March 2018
1 Read

Complete disappearance of ABO antigen-a cause of ABO discrepancy.

Transfusion 2018 01;58(1):5-6

Department of Medical Oncology, Jawaharlal Institute of Post-graduate Medical Education and Research, Puducherry, India.

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January 2018
4 Reads

Comparison of anticancer effect of Pleurotus ostreatus extract with doxorubicin hydrochloride alone and plus thermotherapy on erythroleukemia cell line.

J Complement Integr Med 2017 Dec 19;15(2). Epub 2017 Dec 19.

Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Background Recent studies have introduced Pleurotus ostreatus (Pleurotaceae) as a herbal medicine for treating different types of cancer. This survey utilizes P. ostreatus and doxorubicin hydrochloride (DOX) alone and then with hyperthermia to investigate the erythroleukemia cell line. Read More

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December 2017
13 Reads

Allosteric inhibitor remotely modulates the conformation of the orthestric pockets in mutant IDH2/R140Q.

Sci Rep 2017 Nov 28;7(1):16458. Epub 2017 Nov 28.

Key Laboratory of Drug Targets and Drug Leads for Degenerative Diseases, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.

Neomorphic mutation R140Q in the metabolic enzyme isocitrate dehydrogenase 2 (IDH2) is found to be a driver mutation in cancers. Recent studies revealed that allosteric inhibitors could selectively inhibit IDH2/R140Q and induce differentiation of TF-1 erythroleukemia and primary human AML cells. However, the allosteric inhibition mechanism is not very clear. Read More

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November 2017
14 Reads

Design and synthesis of aminoester heterodimers containing flavone or chromone moieties as modulators of P-glycoprotein-based multidrug resistance (MDR).

Bioorg Med Chem 2018 01 10;26(1):50-64. Epub 2017 Nov 10.

Department of Neuroscience, Psychology, Drug Research and Child's Health - Section of Pharmaceutical and Nutraceutical Sciences, University of Florence, via Ugo Schiff 6, 50019 Sesto Fiorentino (FI), Italy.

In this study, a new series of heterodimers was synthesized. These derivatives are N,N-bis(alkanol)amine aryl esters or N,N-bis(ethoxyethanol)amine aryl esters carrying a methoxylated aryl residue combined with a flavone or chromone moiety. The new compounds were studied to evaluate their P-gp modulating activity on a multidrug-resistant leukemia cell line. Read More

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January 2018
3 Reads

Anti-invasive and antiproliferative effects of Pleurotus ostreatus extract on acute leukemia cell lines.

J Basic Clin Physiol Pharmacol 2018 Jan;29(1):95-102

Department of Biostatistics, Faculty of Medical Sciences, Baqiyatallah University, Tehran, Iran.

Background: Currently, mushrooms have been used in traditional and folk medicines for their therapeutic activities, such as antibiotic, antitumor, anti-inflammatory, anticancer, antileukemic and immunomodulatory actions. This investigation evaluates the anti-invasive, antiproliferative and cytotoxic effects of Pleurotus ostreatus (Pleurotaceae) on leukemia cell lines.

Methods: The proliferation of KG-1 cells was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay after treatment with gradient dilutions of P. Read More

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January 2018
14 Reads

Combined effect of gene dosage and process optimization strategies on high-level production of recombinant human interleukin-3 (hIL-3) in Pichia pastoris fed-batch culture.

Int J Biol Macromol 2018 Mar 4;108:999-1009. Epub 2017 Nov 4.

Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India. Electronic address:

In this work, the combined effects of gene dosage and process optimization strategies were studied to achieve higher hIL-3 expression in Pichia system. The in-vitro multimerization method was used to generate various Pichia X-33 transformants having multi-copy expression cassettes. The quantitative polymerase chain reaction (qPCR) strategy was used to further confirm the genome integration of hIL-3 expression cassette. Read More

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March 2018
6 Reads

CRISPR/Cas9 knockouts reveal genetic interaction between strain-transcendent erythrocyte determinants of invasion.

Proc Natl Acad Sci U S A 2017 10 19;114(44):E9356-E9365. Epub 2017 Oct 19.

Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, MA 02115;

During malaria blood-stage infections, parasites interact with the RBC surface to enable invasion followed by intracellular proliferation. Critical factors involved in invasion have been identified using biochemical and genetic approaches including specific knockdowns of genes of interest from primary CD34 hematopoietic stem cells (cRBCs). Here we report the development of a robust in vitro culture system to produce RBCs that allow the generation of gene knockouts via CRISPR/Cas9 using the immortal JK-1 erythroleukemia line. Read More

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October 2017
11 Reads

Genome Editing of Erythroid Cell Culture Model Systems.

Methods Mol Biol 2018 ;1698:245-257

School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.

Genome editing to introduce specific mutations or to knock out genes in model cell systems has become an efficient platform for research in the fields of molecular biology, genetics, and cell biology. With recent rapid improvements in genome editing techniques, bench-top manipulation of the genome in cell culture has become progressively easier. The application of this knowledge to erythroid cell culture systems now allows the rapid analysis of the downstream effects of virtually any engineered gene disruption or modification in cell systems. Read More

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June 2018
10 Reads

[Anti-Tumor Effect of Rigosertib on HEL and K562 Cells and Its Related Mechanism].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2017 Oct;25(5):1362-1366

Department of Hematology, Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, China.

Objective: To investigate the effects of rigosertib on the apoptosis, proliferation and cell cycle of HEL and K562 cells.

Methods: The HEL and K562 cells were treated with different concentration of rigosertib at different time points, the cell apoptosis, proliferation and cycle were determined by using flow cytometry with Annexin V/PI double staining, WST-1 method and 7-AAD assay, respectively. Intracellular signaling proteins were detected by flow cytometry (FCM). Read More

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October 2017
13 Reads

Dimethyl fumarate increases fetal hemoglobin, provides heme detoxification, and corrects anemia in sickle cell disease.

JCI Insight 2017 Oct 19;2(20). Epub 2017 Oct 19.

Hematology Research, Bioverativ, Waltham, Massachusetts, USA.

Sickle cell disease (SCD) results from a point mutation in the β-globin gene forming hemoglobin S (HbS), which polymerizes in deoxygenated erythrocytes, triggering recurrent painful vaso-occlusive crises and chronic hemolytic anemia. Reactivation of fetal Hb (HbF) expression ameliorates these symptoms of SCD. Nuclear factor (erythroid derived-2)-like 2 (Nrf2) is a transcription factor that triggers cytoprotective and antioxidant pathways to limit oxidative damage and inflammation and increases HbF synthesis in CD34+ stem cell-derived erythroid progenitors. Read More

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October 2017
29 Reads

MYCN contributes to the malignant characteristics of erythroleukemia through EZH2-mediated epigenetic repression of p21.

Cell Death Dis 2017 10 12;8(10):e3126. Epub 2017 Oct 12.

Department of Hematology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai 200233, China.

MYC proto-oncogene family including c-myc and n-myc (MYCN) are critical for normal cell development and tumorigenesis. Overexpression of c-myc causes acute erythroleukemia in vivo. However, the role of MYCN in acute erythroleukemia remains poorly understood. Read More

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October 2017
27 Reads

Erythroleukemia-historical perspectives and recent advances in diagnosis and management.

Blood Rev 2018 Mar 18;32(2):96-105. Epub 2017 Sep 18.

Department of Leukemia, The University of Texas, MD Anderson Cancer Center, Houston, TX, USA. Electronic address:

Acute erythroleukemia is a rare form of acute myeloid leukemia recognized by its distinct phenotypic attribute of erythroblastic proliferation. After a century of its descriptive history, many diagnostic, prognostic, and therapeutic implications relating to this unique leukemia subset remain uncertain. The rarity of the disease and the simultaneous involvement of its associated myeloid compartment have complicated in vitro studies of human erythroleukemia cell lines. Read More

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March 2018
20 Reads

"Hof" in pronormoblasts: pure erythroid leukemia mimicking plasma cell myeloma.

Blood 2017 09;130(13):1600

University of Texas MD Anderson Cancer Center.

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September 2017
12 Reads

[Chemical constituents from Phellinus igniarius and their anti-tumor activity in vitro].

Zhongguo Zhong Yao Za Zhi 2016 Aug;41(16):3042-3048

School of Pharmacy, Anhui Medical University, Hefei 230032, China.

Eleven compounds were isolated and purified from Phellinus igniarius by column chromatography on silica gel, Sephedax LH-20, RP-8, MCI and preparative TLC. Their structures were identified as 3α-hydroxyfriedel-2-one (1), 3-hydroxyfriedel-3-en-2-one (2), ergosta-4, 6, 8 (14), 22-tetraen-3-one (3), ergosterol peroxide (4), uracil (5), uridine (6), 4-(3, 4-dihydroxyphenyl)-3-butene-2-one (7), protocatechualdehyde (8), inotilone (9), inoscavinA (10) and phellibaumin E (11), respectively, on the basis of NMR and MS data analysis. Among them, compounds 1, 2, 5, and 6 were firstly obtained from this genus. Read More

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August 2016
13 Reads

Senescence is a Spi1-induced anti-proliferative mechanism in primary hematopoietic cells.

Haematologica 2017 11 14;102(11):1850-1860. Epub 2017 Sep 14.

Institut Gustave Roussy, Université Paris-Saclay, Villejuif, France

Transcriptional deregulation caused by epigenetic or genetic alterations is a major cause of leukemic transformation. The Spi1/PU.1 transcription factor is a key regulator of many steps of hematopoiesis, and limits self-renewal of hematopoietic stem cells. Read More

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November 2017
15 Reads

Antioxidant and cytotoxic activity of propolis of Plebeia droryana and Apis mellifera (Hymenoptera, Apidae) from the Brazilian Cerrado biome.

PLoS One 2017 12;12(9):e0183983. Epub 2017 Sep 12.

Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil.

Propolis is a complex bioactive mixture produced by bees, known to have different biological activities, especially in countries where there is a rich biodiversity of plant species. The objective of this study was to determine the chemical composition and evaluate the antioxidant and cytotoxic properties of Brazilian propolis from the species Plebeia droryana and Apis mellifera found in Mato Grosso do Sul, Brazil. In the ethanolic extracts of P. Read More

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October 2017
17 Reads