8,174 results match your criteria Erythroleukemia

[Hypoxia promotes erythroid differentiation of human K562 cells by upregulating endothelial PAS domain-containing protein 1 (EPAS1) expression].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2022 Jun;38(6):494-500

Air Force Clinical College, Anhui Medical University, Beijing 100142; Department of Transfusion, Air Force Medical Center, Beijing 100142, China. *Corresponding author, E-mail:

Objective To investigate the role of endothelial PAS domain-containing protein 1 (EPAS1) gene encoded hypoxia-inducible factor 2α (HIF-2α) in erythroid differentiation of K562 human erythroleukemia cells under hypoxic conditions. Methods K562 cells were treated with 40 μmol/L of hemin and 0.1 ng/mL of cytarabine for erythroid differentiation. Read More

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Detachment of Hexokinase II From Mitochondria Promotes Collateral Sensitivity in Multidrug Resistant Chronic Myeloid Leukemia Cells.

Front Oncol 2022 26;12:852985. Epub 2022 May 26.

Laboratório de Trombose e Câncer, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.

Chronic Myeloid Leukemia is a neoplastic disease characterized by the abnormal expansion of hematopoietic cells with compromised functions. Leukemic cells often display a multidrug resistance phenotype, enabling them to evade a number of structurally unrelated cytotoxic compounds. One of those mechanisms relies on the high expression of efflux transporters, such as the ABC proteins, whose activity depends on the hydrolysis of ATP to reduce intracellular drug accumulation. Read More

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Differential characteristics of TP53 alterations in pure erythroid leukemia arising after exposure to cytotoxic therapy.

Leuk Res 2022 Jul 17;118:106860. Epub 2022 May 17.

Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX, United States. Electronic address:

Pure erythroid leukemia (PEL) is a rare acute leukemia with a dismal prognosis. TP53 mutations are a dominant feature of PEL, but the characteristics of TP53 alterations in PEL without prior exposure to cytotoxic therapy (d-PEL) or with such exposure (t-PEL) is unknown. We studied 25 patients with TP53-mutated PEL including 16 d-PEL and 9 t-PEL. Read More

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Anti-Cancer Role of Dendrosomal Nano Solanine in Chronic Myelogenous Leukemia Cell Line through Attenuation of PI3K/AKT/mTOR Signaling Pathway and Inhibition of hTERT Expression.

Curr Mol Pharmacol 2022 May 16. Epub 2022 May 16.

Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Background Solanine was primarily known as a toxic compound. Nonetheless, recently the apoptotic role of solanine through suppression of PI3K/AKT/mTOR signaling pathway has been shown against many malignancies except chronic myelogenous leukemia (CML). Sustaining aforementioned prosurvival pathway, BCR-ABL fused oncoprotein in CML activates NF-kB and c-MYC for apparent immortalizing factor hTERT. Read More

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Pre-mRNA splicing factor U2AF2 recognizes distinct conformations of nucleotide variants at the center of the pre-mRNA splice site signal.

Nucleic Acids Res 2022 05;50(9):5299-5312

Department of Biochemistry and Biophysics, and the Center for RNA Biology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.

The essential pre-mRNA splicing factor U2AF2 (also called U2AF65) identifies polypyrimidine (Py) tract signals of nascent transcripts, despite length and sequence variations. Previous studies have shown that the U2AF2 RNA recognition motifs (RRM1 and RRM2) preferentially bind uridine-rich RNAs. Nonetheless, the specificity of the RRM1/RRM2 interface for the central Py tract nucleotide has yet to be investigated. Read More

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BMP2/SMAD pathway activation in JAK2/p53-mutant megakaryocyte/erythroid progenitors promotes leukemic transformation.

Blood 2022 Jun;139(25):3630-3646

Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY.

Leukemic transformation (LT) of myeloproliferative neoplasm (MPN) has a dismal prognosis and is largely fatal. Mutational inactivation of TP53 is the most common somatic event in LT; however, the mechanisms by which TP53 mutations promote LT remain unresolved. Using an allelic series of mouse models of Jak2/Trp53 mutant MPN, we identify that only biallelic inactivation of Trp53 results in LT (to a pure erythroleukemia [PEL]). Read More

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Current insights into the role of Fli-1 in hematopoiesis and malignant transformation.

Cell Mol Life Sci 2022 Feb 28;79(3):163. Epub 2022 Feb 28.

Department of Medicine, University of Toronto, Toronto, ON, Canada.

Fli-1, a member of the ETS family of transcription factors, was discovered in 1991 through retroviral insertional mutagenesis as a driver of mouse erythroleukemias. In the past 30 years, nearly 2000 papers have defined its biology and impact on normal development and cancer. In the hematopoietic system, Fli-1 controls self-renewal of stem cells and their differentiation into diverse mature blood cells. Read More

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February 2022

Flumioxazin, a PPO inhibitor: A weight-of-evidence consideration of its mode of action as a developmental toxicant in the rat and its relevance to humans.

Toxicology 2022 04 30;472:153160. Epub 2022 Mar 30.

Consultant, Harrington House, Brighton BN1 6RE, UK.

Flumioxazin, is a herbicide that has inhibitory activity on protoporphyrinogen oxidase (PPO), a key enzyme in the biosynthetic pathway for heme. Flumioxazin induces anemia and developmental toxicity in rats, including ventricular septal defect and embryofetal death. Studies to elucidate the mode of action (MOA) of flumioxazin as a developmental toxicant and to evaluate its relevance to humans have been undertaken. Read More

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[Molecular pathogenesis and therapeutic targets in acute erythroid leukemia].

June Takeda

Rinsho Ketsueki 2022 ;63(2):121-133

Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University.

Acute erythroid leukemia (AEL) is a unique subtype of acute myeloid leukemia characterized by erythroid predominance and dysplasia. It is classified into two subtypes: pure erythroid (PEL) and erythroid/myeloid (EML) phenotypes. To understand the mechanism of the erythroid dominant phenotype of AEL and identify potential therapeutic targets for AEL, we analyzed 105 AEL and 214 non-AEL cases using whole-genome/exome and/or targeted-capture sequencing, with SNP probes for detecting copy number abnormalities. Read More

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A Rare STXBP2 Mutation in Severe COVID-19 and Secondary Cytokine Storm Syndrome.

Life (Basel) 2022 Jan 20;12(2). Epub 2022 Jan 20.

Department of Pediatrics, Division of Rheumatology, University of Alabama at Birmingham, Birmingham, AL 35233-1711, USA.

Background: Primary (familial) hemophagocytic lymphohistiocytosis (pHLH) is a potentially lethal syndrome of infancy, caused by genetic defects in natural killer (NK) cell and CD8 T cell cytotoxicity, leading to hyperinflammation, elevated cytokine levels, and a disorganized immune response resulting in multi-organ system failure and frequently death. Secondary HLH (sHLH) can be triggered in the setting of malignances, diseases of chronic immune system activation, or by infectious etiologies. While pHLH is usually a result of homozygous gene mutations, monoallelic hypomorphic and dominant-negative mutations in pHLH genes have been implicated in sHLH. Read More

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January 2022

Erythroid differentiation in mouse erythroleukemia cells depends on Tmod3-mediated regulation of actin filament assembly into the erythroblast membrane skeleton.

FASEB J 2022 03;36(3):e22220

Department of Biological Sciences, University of Delaware, Newark, Delaware, USA.

Erythroid differentiation (ED) is a complex cellular process entailing morphologically distinct maturation stages of erythroblasts during terminal differentiation. Studies of actin filament (F-actin) assembly and organization during terminal ED have revealed essential roles for the F-actin pointed-end capping proteins, tropomodulins (Tmod1 and Tmod3). Tmods bind tropomyosins (Tpms), which enhance Tmod capping and F-actin stabilization. Read More

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Specific effects of somatic GATA2 zinc finger mutations on erythroid differentiation.

Exp Hematol 2022 04 16;108:26-35. Epub 2022 Feb 16.

Department of Medicine III, University Hospital, LMU Munich, Munich, Germany; German Cancer Consortium, Partner Site Munich, Munich, Germany; German Cancer Research Center, Heidelberg, Germany. Electronic address:

GATA2 zinc-finger (ZF) mutations are associated with distinct entities of myeloid malignancies. The specific distribution of these mutations points toward different mechanisms of leukemogenesis depending on the ZF domain affected. In this study, we compared recurring somatic mutations in ZF1 and ZF2. Read More

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The role of the atypical chemokine receptor CCRL2 in myelodysplastic syndrome and secondary acute myeloid leukemia.

Sci Adv 2022 02 18;8(7):eabl8952. Epub 2022 Feb 18.

Division of Hematological Malignancies, Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University Hospital, Baltimore, MD, USA.

The identification of new pathways supporting the myelodysplastic syndrome (MDS) primitive cells growth is required to develop targeted therapies. Within myeloid malignancies, men have worse outcomes than women, suggesting male sex hormone-driven effects in malignant hematopoiesis. Androgen receptor promotes the expression of five granulocyte colony-stimulating factor receptor-regulated genes. Read More

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February 2022

Functional Evaluation of KEL as an Oncogenic Gene in the Progression of Acute Erythroleukemia.

Oxid Med Cell Longev 2022 30;2022:5885342. Epub 2022 Jan 30.

Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, China.

Acute erythroleukemia (AEL) is an infrequent subtype of acute myeloid leukemia (AML) with worse prognosis. Though the last decade has seen major advances in the novel features and genomic landscape in AEL, there is still a lack of specific therapeutic targets and effective treatment approaches for this disease. Here, we found a novel oncogene KEL that specifically and aberrantly expressed in patients with AEL. Read More

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A rare type of acute leukemia in peripheral blood smear.

Hematol Transfus Cell Ther 2022 Jan 25. Epub 2022 Jan 25.

Hospital Universitario Fundación Jiménez Díaz, Madrid, Spain.

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January 2022

FLI1 regulates inflammation-associated genes to accelerate leukemogenesis.

Cell Signal 2022 04 29;92:110269. Epub 2022 Jan 29.

State Key Laboratory for Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, Guizhou Province, People's Republic of China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academic of Sciences, Guiyang 550014, Guizhou Province, People's Republic of China. Electronic address:

Inflammation plays a critical role in cancer initiation and progression, and is induced by inflammatory factors that are direct target of oncogenes and tumor suppressors. The ETS related transcription factor Fli-1 is involved in the induction and progression of various cancers; yet its role in inflammation is not well-defined. Using RNAseq analysis, we herein demonstrate that FLI1 induces the inflammatory pathway in erythroleukemia cells. Read More

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Quantifying RNA synthesis at rate-limiting steps of transcription using nascent RNA-sequencing data.

STAR Protoc 2022 03 5;3(1):101036. Epub 2022 Jan 5.

KTH Royal Institute of Technology, Department of Gene Technology, Science for Life Laboratory, Stockholm, Sweden.

Nascent RNA-sequencing tracks transcription at nucleotide resolution. The genomic distribution of engaged transcription complexes, in turn, uncovers functional genomic regions. Here, we provide analytical steps to (1) identify transcribed regulatory elements genome-wide, (2) quantify engaged transcription complexes at enhancers, promoter-proximal regions, divergent transcripts, gene bodies, and termination windows, and (3) measure distribution of transcription machineries and regulatory proteins across functional genomic regions. Read More

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GAPDH is involved in the heme-maturation of myoglobin and hemoglobin.

FASEB J 2022 02;36(2):e22099

Department of Inflammation and Immunity, Lerner Research Institute, The Cleveland Clinic, Cleveland, Ohio, USA.

GAPDH, a heme chaperone, has been previously implicated in the incorporation of heme into iNOS and soluble guanylyl cyclase (sGC). Since sGC is critical for myoglobin (Mb) heme-maturation, we investigated the role of GAPDH in the maturation of this globin, as well as hemoglobins α, β, and γ. Utilizing cell culture systems, we found that overexpression of wild-type GAPDH increased, whereas GAPDH mutants H53A and K227A decreased, the heme content of Mb and Hbα and Hbβ. Read More

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February 2022

Use of Stingless Bee Propolis and Geopropolis against Cancer-A Literature Review of Preclinical Studies.

Pharmaceuticals (Basel) 2021 Nov 14;14(11). Epub 2021 Nov 14.

Laboratório de Farmacognosia, Departamento de Farmácia, Campus Bacanga, Universidade Federal do Maranhão, Av. dos Portugueses, 1966, São Luís 65080-805, Maranhão, Brazil.

Cancer is one of the major maladies affecting humankind and remains one of the leading causes of death worldwide. The investigation of the biological activities of stingless bee products, especially propolis and geopropolis, has revealed promising therapeutic properties, especially in the research on new antineoplastic agents. This literature review of preclinical trials, involving biological assays of antitumor activity and identification of the chemical composition of propolis and geopropolis of stingless bee species, describes the cytotoxicity in tumor lineages (breast, lung, ovarian, liver, mouth, pharynx, larynx, colon, stomach, colorectal, cervix, kidney, prostate, melanoma, human glioblastoma, canine osteosarcoma, erythroleukemia, human chronic myelocytic leukemia, and human promyelocytic leukemia) of propolis and geopropolis of 33 species of stingless bees. Read More

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November 2021

The Y14-p53 regulatory circuit in megakaryocyte differentiation and thrombocytopenia.

iScience 2021 Nov 29;24(11):103368. Epub 2021 Oct 29.

Institute of Biomedical Sciences, Academia Sinica, 128 Academia Road, Section 2, Nangang, Taipei 11529, Taiwan.

Thrombocytopenia-absent radius (TAR) syndrome is caused by RBM8A insufficiency. We generated megakaryocyte-specific knockout (KO) mice that exhibited marked thrombocytopenia, internal hemorrhage, and splenomegaly, providing evidence that genetic deficiency of causes a disorder of platelet production. KO mice accumulated low-ploidy immature megakaryocytes in the bone marrow and exhibited defective platelet activation and aggregation. Read More

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November 2021

Identification of Spiro-Fused Pyrrolo[3,4-]pyrrolizines and Tryptanthrines as Potential Antitumor Agents: Synthesis and In Vitro Evaluation.

Int J Mol Sci 2021 Nov 5;22(21). Epub 2021 Nov 5.

Saint-Petersburg National Research Academic University of the Russian Academy of Sciences, 194021 Saint-Petersburg, Russia.

A series of heterocyclic compounds containing a spiro-fused pyrrolo[3,4-]pyrrolizine and tryptanthrin framework have been synthesized and studied as potential antitumor agents. Cytotoxicity of products was screened against human erythroleukemia (K562) and human cervical carcinoma (HeLa) cell lines. Among the screened compounds. Read More

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November 2021

NKL Homeobox Genes NKX2-3 and NKX2-4 Deregulate Megakaryocytic-Erythroid Cell Differentiation in AML.

Int J Mol Sci 2021 Oct 22;22(21). Epub 2021 Oct 22.

Department of Human and Animal Cell Lines, Leibniz-Institute DSMZ, German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany.

NKL homeobox genes encode transcription factors that impact normal development and hematopoietic malignancies if deregulated. Recently, we established an NKL-code that describes the physiological expression pattern of eleven NKL homeobox genes in the course of hematopoiesis, allowing evaluation of aberrantly activated NKL genes in leukemia/lymphoma. Here, we identify ectopic expression of NKL homeobox gene NKX2-4 in an erythroblastic acute myeloid leukemia (AML) cell line OCI-M2 and describe investigation of its activating factors and target genes. Read More

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October 2021

Sildenafil Citrate Downregulates PDE5A mRNA Expression in Women with Recurrent Pregnancy Loss without Altering Angiogenic Factors-A Preliminary Study.

J Clin Med 2021 Oct 29;10(21). Epub 2021 Oct 29.

Department of Biochemistry and Pharmacogenomics, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, Poland.

In our previous study, we showed that sildenafil citrate (SC), a selective PDE5A blocker, modulated NK cell activity in patients with recurrent pregnancy loss, which correlated with positive pregnancy outcomes. It was found that NK cells had a pivotal role in decidualization, angiogenesis, spiral artery remodeling, and the regulation of trophoblast invasion. Thus, in the current study, we determined the effects of SC on angiogenic factor expression and production, as well as idNK cell activity in the presence of nitric synthase blocker L-NMMA. Read More

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October 2021

Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture.

Nat Commun 2021 10 29;12(1):6241. Epub 2021 Oct 29.

Division of Hematology/Oncology, Boston Children's Hospital and Department of Pediatric Oncology, Dana-Farber Cancer Institute (DFCI), Harvard Stem Cell Institute, Harvard Medical School, Boston, MA, USA.

Precise control of gene expression during differentiation relies on the interplay of chromatin and nuclear structure. Despite an established contribution of nuclear membrane proteins to developmental gene regulation, little is known regarding the role of inner nuclear proteins. Here we demonstrate that loss of the nuclear scaffolding protein Matrin-3 (Matr3) in erythroid cells leads to morphological and gene expression changes characteristic of accelerated maturation, as well as broad alterations in chromatin organization similar to those accompanying differentiation. Read More

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October 2021

EloA promotes HEL polyploidization upon PMA stimulation through enhanced ERK1/2 activity.

Platelets 2022 Jul 26;33(5):755-763. Epub 2021 Oct 26.

Laboratory of Radiation Biology, Department of Blood Transfusion, Laboratory Medicine Center, the Second Affiliated Hospital, Army Medical University, Chongqing, China.

Megakaryocytes (MKs) are the unique non-pathological cells that undergo polyploidization in mammals. The polyploid formation is critical for understanding the MK biology, and transcriptional regulation is involved in the differentiation and maturation of MKs. However, little is known about the functions of transcriptional elongation factors in the MK polyploidization. Read More

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Transcriptomic profile of human erythroleukemia cells in response to Sargassum fusiforme polysaccharide and its structure analysis.

Chin J Nat Med 2021 Oct;19(10):784-795

College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China. Electronic address:

Sargassum fusiforme (S. fusiforme) has been used as an ingredient in Chinese herbal medicine for thousands of years. However, there are a limited number of studies concerning its therapeutic mechanism. Read More

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October 2021

Treatment of acute erythroleukaemia with high-dose cytarabine in a cat with feline leukaemia virus infection.

Vet Med Sci 2022 01 2;8(1):9-13. Epub 2021 Oct 2.

College of Veterinary Medicine, Seoul National University, Seoul, Korea.

Erythroleukaemia is a malignant neoplasm of the erythroid lineage that rarely occurs in cats. It is associated with the feline leukaemia virus (FeLV), and owing to the poor prognosis, treatment is rarely reported. A 4-year-old female Korean domestic shorthair cat was presented with hyporexia, fever, lethargy, severe anaemia and rubricytosis. Read More

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January 2022

A rare de novo pure erythroid leukemia with JAK2 R683S mutation.

Ann Hematol 2022 Apr 4;101(4):921-922. Epub 2021 Sep 4.

Department of Pathology and Laboratory Medicine, Division of Hematopathology, Royal Jubilee Hospital, 5th floor, 1952 Bay Street, British Columbia, V8R 1J8, Victoria, Canada.

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Loss of a 7q gene, CUX1, disrupts epigenetically driven DNA repair and drives therapy-related myeloid neoplasms.

Blood 2021 09;138(9):790-805

Department of Pathology.

Therapy-related myeloid neoplasms (t-MNs) are high-risk late effects with poorly understood pathogenesis in cancer survivors. It has been postulated that, in some cases, hematopoietic stem and progenitor cells (HSPCs) harboring mutations are selected for by cytotoxic exposures and transform. Here, we evaluate this model in the context of deficiency of CUX1, a transcription factor encoded on chromosome 7q and deleted in half of t-MN cases. Read More

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September 2021