7,996 results match your criteria Erythroleukemia


Di--lauroyl-decitabine-lipid nanocapsules: toward extending decitabine activity.

Int J Nanomedicine 2019 26;14:2091-2102. Epub 2019 Mar 26.

Micro & Nanomédecines Translationelles - MINT, UNIV Angers, INSERM 1066, CNRS 6021, University of Angers, MINT IBS-CHU, Larrey, 49933 Angers, France,

Background: Acute myeloid leukemia mainly affects adult patients. Complete remission for patients younger than 60 years, who are candidates for standard induction therapy, is achieved in 60%-80% of cases. However, the prognosis is still poor for older patients, who are unfit for intensive chemotherapy, and only a few therapies are available. Read More

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https://www.dovepress.com/di-o-lauroyl-decitabine-lipid-nano
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http://dx.doi.org/10.2147/IJN.S190482DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440450PMC
March 2019
1 Read

Growth Factor Independence (GFI) 1B-mediated transcriptional repression and lineage allocation require Lysine Specific Demethylase (LSD)1-dependent recruitment of the BHC complex.

Mol Cell Biol 2019 Apr 15. Epub 2019 Apr 15.

Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City, UT, U.S.A.

Growth Factor Independence (GFI)1B coordinates assembly of transcriptional repressor complexes comprised of co-repressors and histone modifying enzymes to control gene expression programs governing lineage allocation in hematopoiesis. Enforced expression of GFI1B in K562 erythroleukemia cells favors erythroid over megakaryocytic differentiation, providing a platform to define molecular determinants of binary fate decisions triggered by GFI1B. We deployed proteome-wide proximity labeling to identify factors whose inclusion in GFI1B complexes depends upon GFI1B's obligate effector, Lysine Specific Demethylase (LSD)1. Read More

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http://dx.doi.org/10.1128/MCB.00020-19DOI Listing

Design, synthesis and structure-activity relationship study of aminopyridine derivatives as novel inhibitors of Janus kinase 2.

Bioorg Med Chem Lett 2019 Apr 6. Epub 2019 Apr 6.

Shanghai Key Laboratory of New Drug Design, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science & Technology, Shanghai 200237, China. Electronic address:

Janus Kinase 2 (JAK2) is a kind of intracellular non-receptor protein tyrosine kinase and has been certified as an important target for the treatment of myeloproliferative neoplasms and rheumatoid arthritis. However, the low selectivity and potential safety issues restrict the clinical applications of JAK2 inhibitors. Here we found that crizotinib showed good inhibitory activity against JAK2 by enzymatic assays (IC = 27 nM). Read More

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http://dx.doi.org/10.1016/j.bmcl.2019.04.011DOI Listing

Genomic Analysis Provides Insights into Acute Erythroleukemia.

Authors:

Cancer Discov 2019 Apr 12. Epub 2019 Apr 12.

Acute erythroleukemia (AEL) is a genetically distinct malignancy from AML or MDS. Read More

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http://dx.doi.org/10.1158/2159-8290.CD-RW2019-054DOI Listing

Modulation of the spacer in N,N-bis(alkanol)amine aryl ester heterodimers led to the discovery of a series of highly potent P-glycoprotein-based multidrug resistance (MDR) modulators.

Eur J Med Chem 2019 Mar 27;172:71-94. Epub 2019 Mar 27.

Department of Neuroscience, Psychology, Drug Research and Child's Health - Section of Pharmaceutical and Nutraceutical Sciences, University of Florence, via Ugo Schiff 6, 50019 Sesto Fiorentino (FI), Italy.

In this study, a new series of N,N-bis(alkanol)amine aryl ester heterodimers was synthesized and studied. The new compounds were designed based on the structures of our previous arylamine ester derivatives endowed with high P-gp-dependent multidrug resistance reversing activity on a multidrug-resistant leukemia cell line. All new compounds were active in the pirarubicin uptake assay on the doxorubicin-resistant erythroleukemia K562 cells (K562/DOX). Read More

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http://dx.doi.org/10.1016/j.ejmech.2019.03.054DOI Listing
March 2019
2 Reads

Insertional mutagenesis using the Sleeping Beauty transposon system identifies drivers of erythroleukemia in mice.

Sci Rep 2019 Apr 2;9(1):5488. Epub 2019 Apr 2.

Divisions of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA.

Insertional mutagenesis is a powerful means of identifying cancer drivers in animal models. We used the Sleeping Beauty (SB) transposon/transposase system to identify activated oncogenes in hematologic cancers in wild-type mice and mice that express a stabilized cyclin E protein (termed cyclin ET74AT393A). Cyclin E governs cell division and is misregulated in human cancers. Read More

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http://www.nature.com/articles/s41598-019-41805-x
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http://dx.doi.org/10.1038/s41598-019-41805-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445099PMC
April 2019
3 Reads

Recombinant Human Erythropoietin Proteins Synthesized in Escherichia coli Cells: Effects of Additional Domains on the in vitro and in vivo Activities.

Biochemistry (Mosc) 2019 Jan;84(1):20-32

Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Healthcare of the Russian Federation, Moscow, 123098, Russia.

The aim of this work was to compare biological activities of three variants of bacterially expressed human recombinant erythropoietin (EPO) with additional protein domains: 6His-s-tag-EPO protein carrying the s-tag (15-a.a. oligopeptide from bovine pancreatic ribonuclease A) at the N-terminus and HBD-EPO and EPO-HBD proteins containing heparin-binding protein domains (HBD) of the bone morphogenetic protein 2 from Danio rerio at the N- and C-termini, respectively. Read More

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http://dx.doi.org/10.1134/S0006297919010036DOI Listing
January 2019
1 Read

Genomic subtyping and therapeutic targeting of acute erythroleukemia.

Nat Genet 2019 04 29;51(4):694-704. Epub 2019 Mar 29.

Department of Pathology, St. Jude Children's Research Hospital, Memphis, TN, USA.

Acute erythroid leukemia (AEL) is a high-risk leukemia of poorly understood genetic basis, with controversy regarding diagnosis in the spectrum of myelodysplasia and myeloid leukemia. We compared genomic features of 159 childhood and adult AEL cases with non-AEL myeloid disorders and defined five age-related subgroups with distinct transcriptional profiles: adult, TP53 mutated; NPM1 mutated; KMT2A mutated/rearranged; adult, DDX41 mutated; and pediatric, NUP98 rearranged. Genomic features influenced outcome, with NPM1 mutations and HOXB9 overexpression being associated with a favorable prognosis and TP53, FLT3 or RB1 alterations associated with poor survival. Read More

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http://dx.doi.org/10.1038/s41588-019-0375-1DOI Listing
April 2019
1 Read
29.352 Impact Factor

The 2016 WHO classification of acute myeloid leukemia: What the practicing clinician needs to know.

Authors:
Daniel A Arber

Semin Hematol 2019 Apr 22;56(2):90-95. Epub 2018 Aug 22.

Department of Pathology, University of Chicago, Chicago, IL. Electronic address:

In 2016 a revision of the World Health Organization (WHO) classification of acute myeloid leukemia (AML) was introduced that included changes to several disease categories. The WHO approach results in disease categories that are defined by a combination of clinical, morphologic, immunophenotypic, and genetic features in an attempt to define clinically relevant, biologic entities. This review summarizes the WHO approach as well as the priority of specific features for disease classification. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00371963183009
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http://dx.doi.org/10.1053/j.seminhematol.2018.08.002DOI Listing
April 2019
3 Reads

Indole derivatives as multifunctional drugs: Synthesis and evaluation of antioxidant, photoprotective and antiproliferative activity of indole hydrazones.

Bioorg Chem 2019 Apr 10;85:568-576. Epub 2019 Feb 10.

Department of Life and Environmental Sciences, Unit of Pharmaceutical, Pharmacological and Nutraceutical Sciences, University of Cagliari, via Ospedale 72, Cagliari I-09124, Italy. Electronic address:

Two series of indole derivatives 4-17, 20-22 were easily prepared and assayed for their radical-scavenging ability. Arylidene-1H-indole-2-carbohydrazones showed different extent antioxidant activity in DPPH, FRAP and ORAC assays. Good antioxidant activity is related to the number and position of hydroxyl groups on the arylidene moiety as well as to the presence of methoxy or 4-(diethylamino) group. Read More

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http://dx.doi.org/10.1016/j.bioorg.2019.02.007DOI Listing
April 2019
1 Read

In vitro modulation of multidrug resistance by pregnane steroids and in vivo inhibition of tumour development by 7α-OBz-11α(R)-OTHP-5β-pregnanedione in K562/R7 and H295R cell xenografts.

J Enzyme Inhib Med Chem 2019 Dec;34(1):684-691

a ISPB-Faculté de Pharmacie , Université de Lyon, Université Lyon 1 , Lyon , France.

Synthetic progesterone and 5α/β-pregnane-3,20-dione derivatives were evaluated as in vitro and in vivo modulators of multidrug-resistance (MDR) using two P-gp-expressing human cell lines, the non-steroidogenic K562/R7 erythroleukaemia cells and the steroidogenic NCI-H295R adrenocortical carcinoma cells, both resistant to doxorubicin. The maximal effect in both cell lines was observed for 7α-O-benzoyloxy,11α(R)-O-tetrahydropyranyloxy-5β-pregnane-3,20-dione 4. This modulator co-injected with doxorubicin significantly decreased the tumour size and increased the survival time of immunodeficient mice xenografted with NCI-H295R or K562/R7 cells. Read More

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http://dx.doi.org/10.1080/14756366.2019.1575825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383615PMC
December 2019
3 Reads

Interaction between nectin-1 and the human natural killer cell receptor CD96.

PLoS One 2019 13;14(2):e0212443. Epub 2019 Feb 13.

Department of Biological Sciences, Rowan University, Glassboro, New Jersey, United States of America.

Regulation of Natural Killer (NK) cell activity is achieved by the integration of both activating and inhibitory signals acquired at the immunological synapse with potential target cells. NK cells express paired receptors from the immunoglobulin family which share common ligands from the nectin family of adhesion molecules. The activating receptor CD226 (DNAM-1) binds to nectin-2 and CD155, which are also recognized by the inhibitory receptor TIGIT. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212443PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373967PMC
February 2019
3 Reads

Identification of diterpenoid compounds that interfere with Fli-1 DNA binding to suppress leukemogenesis.

Cell Death Dis 2019 Feb 11;10(2):117. Epub 2019 Feb 11.

State Key Laboratory for Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550025, China.

The ETS transcription factor Fli-1 controls the expression of genes involved in hematopoiesis including cell proliferation, survival, and differentiation. Dysregulation of Fli-1 induces hematopoietic and solid tumors, rendering it an important target for therapeutic intervention. Through high content screens of a library of chemicals isolated from medicinal plants in China for inhibitors of a Fli-1 transcriptional reporter cells, we hereby report the identification of diterpenoid-like compounds that strongly inhibit Fli-1 transcriptional activity. Read More

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http://dx.doi.org/10.1038/s41419-019-1363-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370842PMC
February 2019
5 Reads

Undergraduate lab series using the K562 human leukemia cell line: Model for cell growth, death, and differentiation in an advanced cell biology course.

Biochem Mol Biol Educ 2019 Feb 6. Epub 2019 Feb 6.

Department of Biology, Fairfield University, Fairfield, Connecticut, 06824.

This sequence of labs was developed for an upper level undergraduate cell biology course at Fairfield University. The labs are based on the use of the K562 human erythroleukemia cell line, a model system that is exceptionally amenable to an undergraduate cell biology lab course due to its ease of maintenance and propagation and usefulness for studies of growth, death, and differentiation. The sequence of labs is conducted over a 6-week period, following a series of weekly cell biology labs covering basic cell and molecular biology techniques. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/bmb.21222
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http://dx.doi.org/10.1002/bmb.21222DOI Listing
February 2019
7 Reads

Discovery and evaluation of ZT55, a novel highly-selective tyrosine kinase inhibitor of JAK2 against myeloproliferative neoplasms.

J Exp Clin Cancer Res 2019 Feb 4;38(1):49. Epub 2019 Feb 4.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China.

Background: The JAK2-STAT signaling pathway plays a critical role in myeloproliferative neoplasms (MPN). An activating mutation in JAK2 (V617F) is present in ~ 95% of polycythemia vera, essential thrombocythemia, and primary myelofibrosis cases. This study aims to explore the selective JAK2 inhibitor, evaluate the efficacy and possible mechanism of ZT55 on MPN. Read More

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http://dx.doi.org/10.1186/s13046-019-1062-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360668PMC
February 2019
11 Reads

[Erythroleukemias. Recategorization according to the World Health Organization's criteria].

Medicina (B Aires) 2019 ;79(1):1-5

Servicio de Hematología y Oncología, Hospital de Pediatría Prof. Dr. Juan P. Garrahan, Buenos Aires, Argentina.

Acute leukemia is the most frequent malignant disease in childhood. Acute lymphoblastic leukemia represents 75% and acute myeloblastic leukemia 25% of them. Erythroleukemia is a rare entity, corresponding to less than 5% of acute myeloblastic leukemia. Read More

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January 2019
3 Reads

CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells.

PeerJ 2019 16;7:e6284. Epub 2019 Jan 16.

Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, Spanish National Research Council (CSIC), Madrid, Spain.

Wiskott-Aldrich syndrome (WAS) is a recessive X-linked inmmunodeficiency caused by loss-of-function mutations in the gene encoding the WAS protein (WASp). WASp plays an important role in the polymerization of the actin cytoskeleton in hematopoietic cells through activation of the Arp2/3 complex. In a previous study, we found that actin cytoskeleton proteins, including WASp, were silenced in murine erythroleukemia cells defective in differentiation. Read More

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http://dx.doi.org/10.7717/peerj.6284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339507PMC
January 2019
3 Reads

A recycling anti-transferrin receptor-1 monoclonal antibody as an efficient therapy for erythroleukemia through target up-regulation and antibody-dependent cytotoxic effector functions.

MAbs 2019 04 18;11(3):593-605. Epub 2019 Feb 18.

a IRCM, Institut de Recherche en Cancérologie de Montpellier ; INSERM, U1194, Université de Montpellier, Montpellier , France.

Targeting transferrin receptor 1 (TfR1) with monoclonal antibodies is a promising therapeutic strategy in cancer as tumor cells often overexpress TfR1 and show increased iron needs. We have re-engineered six anti-human TfR1 single-chain variable fragment (scFv) antibodies into fully human scFv-Fcγ1 and IgG1 antibodies. We selected the more promising candidate (H7), based on its ability to inhibit TfR1-mediated iron-loaded transferrin internalization in Raji cells (B-cell lymphoma). Read More

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http://dx.doi.org/10.1080/19420862.2018.1564510DOI Listing
April 2019
7 Reads

Multidrug resistance phenotype: Relation between phenotype induction and its characteristics in erythroleukemia cells.

Cell Biol Int 2019 Feb;43(2):214-219

Programa de Pós-Graduação em Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande- FURG, Av. Itália, Km 8, CEP 96203-900 Rio Grande, RS, Brasil.

Chemotherapy may be followed by multiple drug resistance (MDR). This is an obstacle in the treatment of cancer. It is therefore essential to understand the mechanisms underlying tumor resistance, especially those involved in the cell target/MDR relationship. Read More

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http://dx.doi.org/10.1002/cbin.11095DOI Listing
February 2019
5 Reads

A microfluidic platform utilizing anchored water-in-oil-in-water double emulsions to create a niche for analyzing single non-adherent cells.

Lab Chip 2019 01;19(3):422-431

Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Non-adherent cells play key roles in various biological processes. Studies on this type of cell, especially at single-cell resolution, help reveal molecular mechanisms underlying many biological and pathological processes. The emerging microfluidics technology has developed effective methods for analyzing cells. Read More

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http://dx.doi.org/10.1039/c8lc01130cDOI Listing
January 2019
7 Reads

IL-6 stimulation of DNA replication is JAK1/2 mediated in cross-talk with hyperactivated ERK1/2 signaling.

Cell Biol Int 2019 Feb 7;43(2):192-206. Epub 2019 Jan 7.

Department of Molecular Oncology, Institute for Medical Research, University of Belgrade, Belgrade, Serbia.

Myeloproliferative neoplasms (MPNs) are developing resistance to therapy by JAK1/2 inhibitor ruxolitinib. To explore the mechanism of ruxolitinib's limited effect, we examined the JAK1/2 mediated induction of proliferation related ERK1/2 and AKT signaling by proinflammatory interleukin-6 (IL-6) in MPN granulocytes and JAK2V617F mutated human erythroleukemia (HEL) cells. We found that JAK1/2 or JAK2 inhibition prevented the IL-6 activation of STAT3 and AKT pathways in polycythemia vera and HEL cells. Read More

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http://dx.doi.org/10.1002/cbin.11084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347554PMC
February 2019
6 Reads
1.933 Impact Factor

Synthesis in Escherichia coli and Characterization of Human Recombinant Erythropoietin with Additional Heparin-Binding Domain.

Biochemistry (Mosc) 2018 Oct;83(10):1207-1221

Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, 123098, Russia.

Recombinant human erythropoietin (EPO) with additional N-terminal heparin-binding protein domain (HBD) from bone morphogenetic protein 2 was synthesized in Escherichia coli cells. A procedure for HBD-EPO purification and refolding was developed for obtaining highly-purified HBD-EPO. The structure of recombinant HBD-EPO was close to that of the native EPO protein. Read More

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http://dx.doi.org/10.1134/S0006297918100061DOI Listing
October 2018
2 Reads

Imidazo[1,2-]pyrazole-7-carboxamides Induce Apoptosis in Human Leukemia Cells at Nanomolar Concentrations.

Molecules 2018 Nov 1;23(11). Epub 2018 Nov 1.

Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary.

Leukemia, the malignancy of the hematopoietic system accounts for 10% of cancer cases with poor overall survival rate in adults; therefore, there is a high unmet medical need for the development of novel therapeutics. Eight imidazo[1,2-]pyrazole-7-carboxamides have been tested for cytotoxic activity against five leukemia cell lines: Acute promyelocytic leukemia (HL-60), acute monocytic leukemia (THP-1), acute T-lymphoblastic leukemia (MOLT-4), biphenotypic B myelomonocytic leukemia (MV-4-11), and erythroleukemia (K-562) cells in vitro. Imidazo[1,2-]pyrazole-7-carboxamides hampered the viability of all five leukemia cell lines with different potential. Read More

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http://www.mdpi.com/1420-3049/23/11/2845
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http://dx.doi.org/10.3390/molecules23112845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278434PMC
November 2018
16 Reads

MRTFA augments megakaryocyte maturation by enhancing the SRF regulatory axis.

Blood Adv 2018 Oct;2(20):2691-2703

Department of Cell Biology.

Serum response factor (SRF) is a ubiquitously expressed transcription factor that binds DNA at CArG (CC[A/T]GG) domains in association with myocardin-family proteins (eg, myocardin-related transcription factor A [MRTFA]) or the ternary complex factor family of E26 transformation-specific (ETS) proteins. In primary hematopoietic cells, knockout of either SRF or MRTFA decreases megakaryocyte (Mk) maturation causing thrombocytopenia. The human erythroleukemia (HEL) cell line mimics the effects of MRTFA on Mk maturation, and MRTFA overexpression (MRTFA) in HEL cells enhances megakaryopoiesis. Read More

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http://dx.doi.org/10.1182/bloodadvances.2018019448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6199649PMC
October 2018
13 Reads

Design, synthesis, and biological evaluation of novel nitric oxide releasing dehydroandrographolide derivatives.

Chin J Nat Med 2018 Oct;16(10):782-790

State Key Laboratory of Natural Medicines, Center of Drug Discovery, China Pharmaceutical University, Nanjing 210009, China; Jiangsu Key Laboratory of Drug Discovery for Metabolic Disease, China Pharmaceutical University, Nanjing 210009, China. Electronic address:

A series of new hybrids of dehydroandrographolide (TAD), a biologically active natural product, bearing nitric oxide (NO)-releasing moieties were synthesized and designated as NO-donor dehydroandrographolide. The biological activities of target compounds were studied in human erythroleukemia K562 cells and breast cancer MCF-7 cells. Biological evaluation indicated that the most active compound I-5 produced high levels of NO and inhibited the proliferation of K562 (IC 1. Read More

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http://dx.doi.org/10.1016/S1875-5364(18)30118-3DOI Listing
October 2018
7 Reads

CARM1-mediated methylation of protein arginine methyltransferase 5 represses human γ-globin gene expression in erythroleukemia cells.

J Biol Chem 2018 11 26;293(45):17454-17463. Epub 2018 Sep 26.

From the State Key Laboratory of Pharmaceutical Biotechnology, Department of Hematology, the Affiliated Drum Tower Hospital of Nanjing University Medical School, China-Australia Center for Translational Medicine, School of Life Sciences, Nanjing University, Nanjing 210023, China,

Protein arginine methyltransferase 5 (PRMT5) is a member of the arginine methyltransferase protein family that critically mediates the symmetric dimethylation of Arg-3 at histone H4 (H4R3me2s) and is involved in many key cellular processes, including hematopoiesis. However, the post-translational modifications (PTMs) of PRMT5 that may affect its biological functions remain less well-understood. In this study, using MS analyses, we found that PRMT5 itself is methylated in human erythroleukemia Lys-562 cells. Read More

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http://dx.doi.org/10.1074/jbc.RA118.004028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231142PMC
November 2018
20 Reads
4.573 Impact Factor

A novel role for in primitive erythropoiesis.

Development 2018 10 11;145(19). Epub 2018 Oct 11.

Australian Centre for Blood Diseases, Monash University, Melbourne, VIC 3004, Australia

Stem cell leukemia ( or ) and lymphoblastic leukemia 1 () encode highly related members of the basic helix-loop-helix family of transcription factors that are co-expressed in the erythroid lineage. Previous studies have suggested that is essential for primitive erythropoiesis. However, analysis of single-cell RNA-seq data of early embryos showed that primitive erythroid cells express both and Therefore, to determine whether can function in primitive erythropoiesis, we crossed conditional knockout mice with mice expressing a Cre recombinase under the control of the Epo receptor, active in erythroid progenitors. Read More

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http://dx.doi.org/10.1242/dev.162990DOI Listing
October 2018
3 Reads
6.462 Impact Factor

Reduction of Thermotolerance by Heat Shock Protein 90 Inhibitors in Murine Erythroleukemia Cells.

Biol Pharm Bull 2018 ;41(9):1393-1400

Department of Chemistry, Faculty of Science, Fukuoka University.

Cells induce heat shock proteins (HSPs) against various stress. However, murine erythroleukemia (MEL) cells do not express HSP72, a heat-inducible member of HSP70 family. So, it is of interest to examine how MEL cells respond to heat stress (44°C, 30 min). Read More

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http://dx.doi.org/10.1248/bpb.b18-00190DOI Listing
November 2018
4 Reads

Knockdown of Long Noncoding RNA Plasmacytoma Variant Translocation 1 with Antisense Locked Nucleic Acid GapmeRs Exerts Tumor-Suppressive Functions in Human Acute Erythroleukemia Cells Through Downregulation of C-MYC Expression.

Cancer Biother Radiopharm 2018 Aug 24. Epub 2018 Aug 24.

Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences , Isfahan, Iran .

Objective: Acute erythroleukemia (AEL) is a subtype of acute myeloid leukemia (AML), with no specific treatment. Up- or downregulation of long noncoding RNAs (lncRNAs) is strongly associated with the formation and progression of many malignancies. Plasmacytoma variant translocation 1 (PVT1) is a significantly upregulated lncRNA in AML. Read More

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https://www.liebertpub.com/doi/10.1089/cbr.2018.2510
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http://dx.doi.org/10.1089/cbr.2018.2510DOI Listing
August 2018
29 Reads

Perylenequione Derivatives with Anticancer Activities Isolated from the Marine Sponge-Derived Fungus, sp. SCSIO41014.

Mar Drugs 2018 Aug 14;16(8). Epub 2018 Aug 14.

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.

Seven new secondary metabolites classified as two perylenequinone derivatives ( and ), an altenusin derivative (), two phthalide racemates ( and ), and two phenol derivatives ( and ), along with twenty-one known compounds (⁻) were isolated from cultures of the sponge-derived fungus, sp. SCSIO41014. The structures and absolute configurations of these new compounds (⁻) were determined by spectroscopic analysis, X-ray single crystal diffraction, chiral-phase HPLC separation, and comparison of ECD spectra to calculations. Read More

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http://dx.doi.org/10.3390/md16080280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117713PMC
August 2018
8 Reads

Interleukin 4 is inactivated via selective disulfide-bond reduction by extracellular thioredoxin.

Proc Natl Acad Sci U S A 2018 08 13;115(35):8781-8786. Epub 2018 Aug 13.

Department of Chemistry, Stanford University, Stanford, CA 94305;

Thioredoxin 1 (TRX), an essential intracellular redox regulator, is also secreted by mammalian cells. Recently, we showed that TRX activates extracellular transglutaminase 2 via reduction of an allosteric disulfide bond. In an effort to identify other extracellular substrates of TRX, macrophages derived from THP-1 cells were treated with NP161, a small-molecule inhibitor of secreted TRX. Read More

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http://dx.doi.org/10.1073/pnas.1805288115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126747PMC
August 2018
13 Reads

Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells.

Cytotechnology 2018 Dec 2;70(6):1509-1518. Epub 2018 Aug 2.

Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Science, Isfahan, 81744-176, Iran.

New achievements in the field of cancer treatment are results of recent advances in molecular medicine and gene therapy. Usage of microRNAs (miRNAs) which are small noncoding RNAs is one of the molecular research lines for the diagnosis and treatment of cancers. miRNAs have an important role in post-transcriptional regulation of the gene expression and are involved in cellular activities such as growth, differentiation, cell death and cancer development. Read More

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http://link.springer.com/10.1007/s10616-018-0241-5
Publisher Site
http://dx.doi.org/10.1007/s10616-018-0241-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269365PMC
December 2018
17 Reads

Lam. Decreases Oxidative Stress in Blood Cells and Prevents Doxorubicin-Induced Cardiotoxicity.

Oxid Med Cell Longev 2018 28;2018:2935051. Epub 2018 Jun 28.

Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil.

Doxorubicin (DOX) is an efficient chemotherapeutic agent, but its clinical application is limited by its cardiotoxicity associated with increased oxidative stress. Thus, the combination of DOX and antioxidants has been encouraged. In this study, we evaluated (I) the chemical composition and antioxidant capacity of aqueous extracts from stem bark (GUEsb) and leaves (GUEl) in 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, 2,2'-azobis(2-amidinopropane) dihydrochloride- (AAPH-) or DOX-induced lipid peroxidation inhibition in human blood cells, and intracellular reactive oxygen species (ROS) quantification using the fluorescent probe dichloro-dihydro-fluorescein diacetate (DCFH-DA) in K562 erythroleukemia cells incubated with GUEsb and stimulated with hydrogen peroxide; (II) the viability of K562 cells and human leukocytes treated with GUEsb in the absence or presence of DOX using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; (III) the acute toxicity of GUEsb; and (IV) the cardioprotective effect of GUEsb in C57Bl/6 mice treated with DOX. Read More

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http://dx.doi.org/10.1155/2018/2935051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046128PMC
October 2018
5 Reads

Induction of apoptosis and necrosis in human acute erythroleukemia cells by inhibition of long non-coding RNA PVT1.

Mol Biol Res Commun 2018 Jun;7(2):89-96

Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Recent advances in molecular medicine have proposed new therapeutic strategies for cancer. One of the molecular research lines for the diagnosis and treatment of cancer is the use of long non-coding RNAs (LncRNAs) which are a class of non-coding RNA molecules longer than 200 base pairs in length that act as the key regulator of gene expression. Different aspects of cellular activities like cell growth, proliferation, differentiation, apoptosis and migration are regulated by lncRNAs. Read More

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http://dx.doi.org/10.22099/mbrc.2018.29081.1316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054779PMC
June 2018
3 Reads

Response to erythropoietin in pediatric patients with chronic kidney disease: insights from an in vitro bioassay.

Pediatr Nephrol 2018 Nov 20;33(11):2123-2129. Epub 2018 Jul 20.

Department of Pediatrics C, Schneider Children's Medical Center of Israel, Sackler Faculty of Medicine, Tel-Aviv University, 14 Kaplan St., Petah Tikva, Israel.

Background: Decreased production of erythropoietin (EPO) is a major cause of anemia associated with chronic kidney disease (CKD). Treatment with recombinant human EPO (rHuEPO) improves patients' quality of life and survival; however, there is a marked variability in response to rHuEPO. At present, no available laboratory test is capable of evaluating responsiveness to EPO treatment. Read More

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http://dx.doi.org/10.1007/s00467-018-4016-1DOI Listing
November 2018
4 Reads

Identification of a Novel Enhancer/Chromatin Opening Element Associated with High-Level γ-Globin Gene Expression.

Mol Cell Biol 2018 Oct 14;38(19). Epub 2018 Sep 14.

Department of Biochemistry and Molecular Biology, College of Medicine, Health Cancer Center, Genetics Institute, Center for Epigenetics, University of Florida, Gainesville, Florida, USA

The organization of the five β-type globin genes on chromosome 11 reflects the timing of expression during erythroid cell development, with the embryonic ε-globin gene being located at the 5' end, followed by the two fetal γ-globin genes, and with the adult β- and δ-globin genes being located at the 3' end. Here, we functionally characterized a DNase I-hypersensitive site (HS) located 4 kb upstream of the Gγ-globin gene (HBG-4kb HS). This site is occupied by transcription factors USF1, USF2, EGR1, MafK, and NF-E2 in the human erythroleukemia cell line K562 and exhibits histone modifications typical for enhancers. Read More

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http://dx.doi.org/10.1128/MCB.00197-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6146835PMC
October 2018
23 Reads

Benzofuran hydrazones as potential scaffold in the development of multifunctional drugs: Synthesis and evaluation of antioxidant, photoprotective and antiproliferative activity.

Eur J Med Chem 2018 Aug 2;156:118-125. Epub 2018 Jul 2.

Department of Life and Environmental Sciences, Unit of Pharmaceutical, Pharmacological and Nutraceutical Sciences, University of Cagliari, Via Ospedale 72, Cagliari, I-09124, Italy. Electronic address:

New benzofuranhydrazones 3-12 were easily prepared and assayed for their radical-scavenging ability. Hydrazones 3-12 showed different extent antioxidant activity in DPPH, FRAP and ORAC assays. Good antioxidant activity is related to the number and position of hydroxyl groups on the arylidene moiety. Read More

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http://dx.doi.org/10.1016/j.ejmech.2018.07.001DOI Listing
August 2018
7 Reads

A novel synthesized 3', 5'-diprenylated chalcone mediates the proliferation of human leukemia cells by regulating apoptosis and autophagy pathways.

Biomed Pharmacother 2018 Oct 11;106:794-804. Epub 2018 Jul 11.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province, Chinese Academy of Sciences, Guiyang, 550014, China. Electronic address:

Leukemia is a hematologic malignancy with poor prognosis in humans and chemotherapy is the main strategy for treating leukemia patients. Novel drugs with better selectivity and lower toxicity are required for the treatment of patients. A novel 3',5'-diprenylated chalcone, (E)-1-(2-hydroxy-4-methoxy-3,5-diprenyl) phenyl-3-(3- pyridinyl)-propene-1-one (C10) is a potential new anti-leukemia agent. Read More

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http://dx.doi.org/10.1016/j.biopha.2018.06.153DOI Listing
October 2018
22 Reads

C-phycocyanin to overcome the multidrug resistance phenotype in human erythroleukemias with or without interaction with ABC transporters.

Biomed Pharmacother 2018 Oct 11;106:532-542. Epub 2018 Jul 11.

Laboratório de Cultura Celular, ICB, FURG, RS, Brazil; Programa de Pós-Graduação em Ciências Fisiológicas, ICB, FURG, RS, Brazil.

The phenotype of multidrug resistance (MDR) is one of the main causes of chemotherapy failure. Our study investigated the effect of C-phycocyanin (C-PC) in three human erythroleukemia cell lines with or without the MDR phenotype: K562 (non-MDR; no overexpression of drug efflux proteins), K562-Lucena (MDR; overexpression of ATP-binding cassette, sub-family B/ABCB1), and FEPS (MDR; overexpression of ABCB1 and ATP-binding cassette, sub-family C/ABCC1). Using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, we showed that 20 and 200 μg/mL C-PC decreased K562 viable cells after 24 h and 200 μg/mL C-PC decreased K562-Lucena cell proliferation after 48 h. Read More

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http://dx.doi.org/10.1016/j.biopha.2018.06.145DOI Listing
October 2018
3 Reads

Pure erythroid leukemia in a polymyositis patient treated with azathioprine.

Rare Tumors 2018 14;10:2036361318773847. Epub 2018 May 14.

Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Kagawa University, Miki, Japan.

Acute erythroid leukemia, also known as acute myeloid leukemia-M6, may be associated with previous chemotherapy or immunosuppressive therapy. For 10 years, a 69-year-old Japanese female patient with pure erythroid leukemia (or acute myeloid leukemia-M6b) was treated for polymyositis with 50-100 mg/day azathioprine. She complained of dyspnea with low-grade fever and was diagnosed as having pure erythroid leukemia. Read More

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http://dx.doi.org/10.1177/2036361318773847DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5954579PMC
May 2018
6 Reads

Selective dissociation between LSD1 and GFI1B by a LSD1 inhibitor NCD38 induces the activation of super-enhancer in erythroleukemia cells.

Oncotarget 2018 Apr 20;9(30):21007-21021. Epub 2018 Apr 20.

Department of Medicine, Shiga University of Medical Science, Otsu, Shiga, Japan.

Lysine-specific demethylase 1 (LSD1) is a histone modifier for transcriptional repression involved in the regulation of hematopoiesis. We previously reported that a LSD1 inhibitor NCD38 induces transdifferentiation from erythroid lineage to granulomonocytic lineage and exerts anti-leukemia effect through de-repression of the specific super-enhancers of hematopoietic regulators including in a human erythroleukemia cell line, HEL. However, the mechanistic basis for this specificity of NCD38 has remained unclear. Read More

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http://dx.doi.org/10.18632/oncotarget.24774DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940392PMC
April 2018
13 Reads

Shifting of erythroleukemia to myelodysplastic syndrome according to the revised WHO classification: Biologic and cytogenetic features of shifted erythroleukemia.

Leuk Res 2018 07 30;70:13-19. Epub 2018 Apr 30.

Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea. Electronic address:

The 2016 revision of the World Health Organization (WHO) classification of tumours of haematopoietic and lymphoid tissues was published. According to 2016 WHO criteria, diagnostic criteria of acute erythroid leukemia was revised. We reassessed 34 de novo acute erythroid leukemia (AEL) diagnosed by 2008 WHO criteria, according to 2016 WHO criteria. Read More

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http://dx.doi.org/10.1016/j.leukres.2018.04.015DOI Listing
July 2018
9 Reads

Psychoneuroimmunology and Natural Killer Cells: The Chromium-Release Whole-Blood Assay.

Methods Mol Biol 2018 ;1781:209-220

Institute for Neuro-Immune Medicine, Nova Southeastern University, Ft. Lauderdale, FL, USA.

Natural killer (NK) cells are an essential component of innate immunity. These lymphocytes are also sensitive barometers of the effects of endogenous and exogenous stressors on the immune system. This chapter describes a chromium (Cr)-release bioassay designed to measure to the target cell killing capacity of NK cells (NKCC). Read More

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http://dx.doi.org/10.1007/978-1-4939-7828-1_12DOI Listing
February 2019
4 Reads

Modulating the expression of Chtop, a versatile regulator of gene-specific transcription and mRNA export.

RNA Biol 2018 11;15(7):849-855. Epub 2018 May 11.

a Department of Applied Biological Science , United Graduate School of Agriculture, Tokyo University of Agriculture and Technology , Fuchu , Tokyo , Japan.

Chtop binds competitively to the arginine methyltransferases PRMT1 and PRMT5, thereby promoting the asymmetric or symmetric methylation of arginine residues, respectively. In cooperation with PRMT1, Chtop activates transcription of certain gene groups, such as the estrogen-inducible genes in breast cancer cells, the 5-hydroxymethylcytosine-modified genes involved in glioblastomagenesis, or the Zbp-89-dependent genes in erythroleukemia cells. Chtop also represses expression of the fetal γ-globin gene. Read More

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https://www.tandfonline.com/doi/full/10.1080/15476286.2018.1
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http://dx.doi.org/10.1080/15476286.2018.1465795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161726PMC
December 2018
24 Reads

Defective RAB1B-related megakaryocytic ER-to-Golgi transport in RUNX1 haplodeficiency: impact on von Willebrand factor.

Blood Adv 2018 04;2(7):797-806

Sol Sherry Thrombosis Research Center and.

Patients with RUNX1 haplodeficiency have thrombocytopenia, platelet dysfunction, and deficiencies of α-granules and dense granules. Platelet expression profiling of a patient with a heterozygous mutation (c.969-323G>T) revealed decreased , which encodes a small G protein. Read More

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http://dx.doi.org/10.1182/bloodadvances.2017014274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894258PMC
April 2018
5 Reads

Drug-DNA adducts as biomarkers for metabolic activation of the nitro-aromatic nitrogen mustard prodrug PR-104A.

Biochem Pharmacol 2018 08 7;154:64-74. Epub 2018 Apr 7.

Auckland Cancer Society Research Centre, The University of Auckland, Auckland, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland, New Zealand.

PR-104A is a clinical-stage nitrogen mustard prodrug that is activated for DNA alkylation by reduction of a nitro group to the corresponding hydroxylamine (PR-104H) or amine (PR-104M). Metabolic reduction is catalysed by flavoreductases such as cytochrome P450 oxidoreductase (POR) under hypoxia, or by aldo-ketoreductase 1C3 (AKR1C3) independently of hypoxia. The unstable reduced metabolites are challenging to measure in biological samples, and biomarkers of the metabolic activation of PR-104A have not been used in the clinical evaluation of PR-104 to date. Read More

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http://dx.doi.org/10.1016/j.bcp.2018.04.004DOI Listing
August 2018
9 Reads

Molecular evidence of JAK2 p.V617F mutated pure erythroid leukemia arising from polycythemia vera.

Virchows Arch 2018 07 2;473(1):131-135. Epub 2018 Apr 2.

Department of Pathology, The Johns Hopkins University School of Medicine, 600 N. Wolfe St., Pathology Building, Room 401, Baltimore, MD, 21287, USA.

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http://dx.doi.org/10.1007/s00428-018-2347-8DOI Listing
July 2018
6 Reads

NF90/ILF3 is a transcription factor that promotes proliferation over differentiation by hierarchical regulation in K562 erythroleukemia cells.

PLoS One 2018 28;13(3):e0193126. Epub 2018 Mar 28.

Pulmonary and Critical Care Medicine, Stanford University School of Medicine, Stanford, California, United States of America.

NF90 and splice variant NF110 are DNA- and RNA-binding proteins encoded by the Interleukin enhancer-binding factor 3 (ILF3) gene that have been established to regulate RNA splicing, stabilization and export. The roles of NF90 and NF110 in regulating transcription as chromatin-interacting proteins have not been comprehensively characterized. Here, chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) identified 9,081 genomic sites specifically occupied by NF90/NF110 in K562 cells. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0193126PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5873942PMC
June 2018
11 Reads

High-level embryonic globin production with efficient erythroid differentiation from a K562 erythroleukemia cell line.

Exp Hematol 2018 06 7;62:7-16.e1. Epub 2018 Mar 7.

Sickle Cell Branch, National Heart Lung and Blood Institute/National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.

A reliable cell line capable of robust in vitro erythroid differentiation would be useful to investigate red blood cell (RBC) biology and genetic strategies for RBC diseases. K562 cells are widely utilized for erythroid differentiation; however, current differentiation methods are insufficient to analyze globin proteins. In this study, we sought to improve erythroid differentiation from K562 cells to enable protein-level globin analysis. Read More

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http://dx.doi.org/10.1016/j.exphem.2018.02.007DOI Listing
June 2018
12 Reads