242,184 results match your criteria Erythrocyte Alloimmunization and Pregnancy


Geodorum densiflorum rhizome lectin inhibits Ehrlich ascites carcinoma cell growth by inducing apoptosis through the regulation of BAX, p53 and NF-κB genes expression.

Int J Biol Macromol 2018 Dec 4. Epub 2018 Dec 4.

Department of Biochemistry and Molecular Biology, Faculty of Science, University of Rajshahi, Rajshahi 6205, Bangladesh. Electronic address:

A lectin with a molecular mass of 12 ± 1 kDa was isolated for the first time from Geodorum densiflorum (Lam.) rhizome (GDL). The lectin exhibited hemagglutination activity both in mice and human erythrocytes which was inhibited by 4-nitrophenyl-β-D-glucopyranoside among the tested 26 sugars. Read More

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December 2018

Global analysis of erythroid cells redox status reveals the involvement of Prdx1 and Prdx2 in the severity of beta thalassemia.

PLoS One 2018 6;13(12):e0208316. Epub 2018 Dec 6.

Universidade Federal de São Carlos (UFSCar), Departamento de Genética e Evolução, São Carlos, Brazil.

β-thalassemia is a worldwide distributed monogenic red cell disorder, characterized by an absent or reduced beta globin chain synthesis. The unbalance of alpha-gamma chain and the presence of pathological free iron promote severe oxidative damage, playing crucial a role in erythrocyte hemolysis, exacerbating ineffective erythropoiesis and decreasing the lifespan of red blood cells (RBC). Catalase, glutathione peroxidase and peroxiredoxins act together to protect RBCs from hydrogen peroxide insult. Read More

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December 2018

Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution.

PLoS One 2018 6;13(12):e0207950. Epub 2018 Dec 6.

Gynecological Endocrinology and Reproductive Medicine, Medical University Innsbruck, Innsbruck, Austria.

Introduction: Extracellular vesicles (EV) are shed from a broad variety of cells and play an important role in activation of coagulation, cell to cell interaction and transport of membrane components. They are usually measured as circulating EV in peripheral blood (PB) and other body fluids. However, little is known about the distribution, presence and impact of EV and their subpopulations in bone marrow (BM). Read More

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December 2018

Septic Arthritis of the Hip-Risk Factors Associated With Secondary Surgery.

J Am Acad Orthop Surg 2018 Dec 4. Epub 2018 Dec 4.

From the Department of Orthopaedics (Dr. R. F. Murphy, Dr. Plumblee, Dr. Barfield, and Dr. Dow), Medical University of South Carolina, Charleston SC, Children's Orthopaedics of Atlanta, Atlanta, GA (Dr. J. S. Murphy, Mr. Fuerstenau), the Department of Orthopaedics and Biomedical Engineering, University of Tennessee-Campbell Clinic, Memphis, TN (Dr. Spence and Dr. Kelly), and Shriner's Hospital for Children, Springfield, MA (Dr. Mooney).

Introduction: Septic arthritis of the hip (SAH) is a common condition encountered by pediatric orthopaedic surgeons and is treated with arthrotomy and irrigation. Depending on the response to initial treatment, some patients require surgical treatment beyond the index procedure. The purpose of this study was to investigate risk factors for repeat surgical intervention after initial arthrotomy for presumed SAH. Read More

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December 2018
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Trisomy silencing by XIST normalizes Down syndrome cell pathogenesis demonstrated for hematopoietic defects in vitro.

Nat Commun 2018 Dec 5;9(1):5180. Epub 2018 Dec 5.

Department of Neurology and Pediatrics, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.

We previously demonstrated that an integrated XIST transgene can broadly repress one chromosome 21 in Down syndrome (DS) pluripotent cells. Here we address whether trisomy-silencing can normalize cell function and development sufficiently to correct cell pathogenesis, tested in an in vitro model of human fetal hematopoiesis, for which DS cellular phenotypes are best known. XIST induction in four transgenic clones reproducibly corrected over-production of megakaryocytes and erythrocytes, key to DS myeloproliferative disorder and leukemia. Read More

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December 2018

The lipid peroxidation in patients with nephrolithiasis before and after extracorporeal shock wave lithotripsy.

Future Med Chem 2018 Dec 6. Epub 2018 Dec 6.

Department of General Biochemistry, Faculty of Biology & Environmental Protection, University of Łódź, Pomorska 141/3, 90-236 Łódź, Poland.

Aim: To evaluate the level of lipid peroxidation in patients with nephrolithiasis before and after extracorporeal shock wave lithotripsy (ESWL).

Materials & Methods: Isoprostane concentration (8-isoPGF) was measured in urine, and thiobarbituric acid reactive substance production in serum and erythrocytes. In addition, the concentrations of selected compounds (uric acid, glucose and creatinine) were measured in serum. Read More

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December 2018

Evaluation of Antioxidant Capacity, Protective Effect on Human Erythrocytes and Phenolic Compound Identification in Two Varieties of Plum Fruit (Spondias spp.) by UPLC-MS.

Molecules 2018 Dec 4;23(12). Epub 2018 Dec 4.

Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora. Encinas y Rosales s/n, 83000 Hermosillo, Sonora, México.

Plum edible part was used to obtained extracts by during a 4 h maceration process using three different solvents (ethanol, methanol and water) for the determination of total phenols and flavonoids, antioxidant capacity by (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hemolysis inhibition in human blood assays. Subsequently, phenolic compounds were identified using ultra-performance liquid chromatography (UPLC-MS). The results indicated that the ethanolic extract of plum fruit being a good source of phenolic (12⁻18 mg GAE/g FW) and flavonoids (2. Read More

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December 2018
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Molecular genotyping of G6PD mutations and Duffy blood group in Afro-descendant communities from Brazilian Amazon.

Genet Mol Biol 2018 Nov 29. Epub 2018 Nov 29.

Laboratório de Genética Humana e Médica, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.

Glucose-6-phosphate dehydrogenase deficiency (G6PDd) and Duffy-negative blood group are two red blood cells variants that confer protection against malaria. In this study, the distribution of the most common G6PD variants (G6PD*A-, GGPD*A and G6PD Mediterranean) and the major alleles of the Duffy blood group (FY*A, FY*B and FY*BES) were investigated in an Afro-descendant population from state of Pará, Brazilian Amazon. G6PD variants and Duffy blood group alleles were determined by TaqMan SNP genotyping assay. Read More

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November 2018

Post Dengue Fever Epidural Abscess With or Without Spondylodiscitis: A Case Series of Eight Patients in a Single Season in a Single Center: Does the Dengue Have a Predilection for the Neural Tissue?

Spine (Phila Pa 1976) 2018 Dec;43(24):E1454-E1462

Aster MIMS Hospital, Calicut, Kerala, India.

Study Design: A retrospective case series.

Objective: The aim of this study was to report the immunosuppression and secondary bacterial infection after dengue viral infection and the predilection of these infections in the spine. This can trigger further research in to this fact. Read More

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December 2018

Low-molecular-weight heparin followed by rivaroxaban or not for the prevention of deep venous thromboembolism after total knee arthroplasty.

Blood Coagul Fibrinolysis 2018 Nov 29. Epub 2018 Nov 29.

Department of Orthopedics, The Second Xiangya Hospital.

: To observe the effect of LMWH followed by rivaroxaban for the prevention of DVT after TKA. Between June 2015 and November 2017 as well as in accordance with the inclusion criteria, 118 patients undergoing TKA were randomly allocated to two groups. Patients in group A were applied LMWH, whereas patients in group B received LMWH followed by rivaroxaban postoperatively. Read More

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November 2018
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Quantification of thiopurine nucleotides in erythrocytes and clinical application to pediatric acute lymphoblastic leukemia.

Ther Drug Monit 2018 Nov 28. Epub 2018 Nov 28.

Department of Laboratory Medicine.

Introduction: Concentrations of 6-thioguanine (6TG) nucleotides and 6-methylmercaptopurine (6MMP) nucleotides in RBCs were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS). This assay was validated for clinical use and was applied to blood samples from patients taking mercaptopurine (6MP).

Methods: RBCs were hemolyzed and deproteinized using perchloric acid, followed by heating for the hydrolysis of nucleotides, and the resultant base was measured using LC-MS/MS. Read More

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November 2018

Genetic variants of erythropoietin (EPO) and EPO receptor genes in familial erythrocytosis.

Int J Lab Hematol 2018 Dec 3. Epub 2018 Dec 3.

Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.

Objectives: Erythrocytosis is characterized by the expansion of erythrocyte compartment including elevated red blood cell number, hematocrit, and hemoglobin content. Familial erythrocytosis (FE) is a congenital disorder with different genetic background. Type 1 FE is primary FE caused by mutation in erythropoietin receptor gene (EPOR). Read More

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December 2018

Cholesterol and Cardiolipin Importance in Local Anesthetics-Membrane Interactions: The Langmuir Monolayer Study.

J Membr Biol 2018 Nov 30. Epub 2018 Nov 30.

Department of General Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387, Kraków, Poland.

Local anesthetics (LAs) are known to act on membrane level; however, the molecular mechanism of their activity is still not fully understood. One hypothesis holds that these drugs can incorporate into lipid membrane of nerve cells and in this way change conformation of channel proteins responsible for transport of sodium ions. However, the action of anesthetics is not limited to nerve cells. Read More

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November 2018

The Association of Serum Leptin Level and Anthropometric Measures With the Severity of Diabetic Retinopathy in Type 2 Diabetes Mellitus.

Med Hypothesis Discov Innov Ophthalmol 2018 ;7(4):156-162

Poostchi Ophthalmology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

This study was performed to determine the association amongst serum leptin level and anthropometric measures with the severity of Diabetic Retinopathy (DR) in subjects with type 2 Diabetes Mellitus (DM). This case-controlled study was conducted within a one-year period, during year 2016, at outpatient retina ophthalmology clinics of Shiraz, southern Iran. Eighty-three patients with type 2 DM, referring for ophthalmoscopy evaluation, were included. Read More

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January 2018

A Simple Noninvasive Score Predicts Disease Activity and Deep Remission in Ulcerative Colitis.

Inflamm Intest Dis 2018 Nov 24;3(1):16-24. Epub 2018 Jul 24.

Internal Medicine Department, Hepatogastroenterology Division, Zagazig University, Al Ahrar Hospital, Zagazig, Egypt.

Background: There is a need to use noninvasive markers in refining the management of ulcerative colitis to reduce the number of unnecessary colonoscopies, which facilitates the follow-up of activity and the response to treatment.

Aim: Postulation of a sensitive, specific, simple and noninvasive score to monitor disease activity in ulcerative colitis.

Methods: A case-control study was conducted: 168 patients with ulcerative colitis, 40 healthy individuals, and 60 patients for validation. Read More

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November 2018
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Comparison of the delta neutrophil index with procalcitonin, erythrocyte sedimentation rate, and C-reactive protein as predictors of sepsis in patients with acute prostatitis.

Prostate Int 2018 Dec 1;6(4):157-161. Epub 2018 Jun 1.

Department of Urology, Yonsei University College of Medicine, Seoul, Korea.

Background: We investigated the usefulness of inflammatory markers including the delta neutrophil index (DNI), erythrocyte sedimentation rate, C-reactive protein, and procalcitonin as early predictors of sepsis in patients with acute prostatitis (AP). In addition, we evaluated the efficacy of intermittent catheterization for the initial management of acute urinary retention (AUR) in patients with AP.

Materials And Methods: All patients who presented to the emergency department and were admitted to the urology department from January 2011 to December 2013 were retrospectively reviewed. Read More

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December 2018

Comparison of Direct and Remote Ischaemic Preconditioning of Renal Ischaemia Reperfusion Injury in Rats.

Turk J Anaesthesiol Reanim 2018 Dec 6;46(6):453-461. Epub 2018 Sep 6.

Department of Anaesthesiology and Reanimation, Ereğli Hospital, Zonguldak, Turkey.

Objective: One of the methods that can be used to prevent ischaemia reperfusion (IR) injury is ischaemic preconditioning. The aim of this study was to evaluate and compare the effects of remote and direct ischaemic preconditioning (RIPC and DIPC) histopathologically in the rat renal IR injury model.

Methods: After obtaining an approval from the Dokuz Eylül University School of Medicine Ethics Committee, 28 Wistar Albino male rats were divided into four groups. Read More

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December 2018

Erythrocyte Aging, Protection via Vesiculation: An Analysis Methodology via Oscillatory Flow.

Front Physiol 2018 16;9:1607. Epub 2018 Nov 16.

Biological Engineering, University of California, San Diego, La Jolla, CA, United States.

We demonstrate that erythrocyte deformations, specifically of a type as occur in splenic flow (Zhu et al., 2017), and of the type that promote vesiculation can be caused by simple, yet tailored, oscillatory shear flow. We show that such oscillatory shear flow provides an ideal environment to explore a wide variety of metabolic and biochemical effects that promote erythrocyte vesiculation. Read More

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November 2018

Cost-effective Tapering Algorithm in Rheumatoid Arthritis Patients: Combination of Multibiomarker Disease Activity Score and Autoantibody Status.

J Rheumatol 2018 Dec 1. Epub 2018 Dec 1.

From the Friedrich-Alexander-University Erlangen-Nuremberg (FAU), Department of Internal Medicine 3 - Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen, Germany; St. Vincent Hospital, Vinforce Study Group, Medical University of Vienna, Vienna, Austria; Institutio de Rheumatologia, São Paolo, Brazil; University of Würzburg, Internal Medicine 2, Würzburg; University Medical Center Freiburg, Rheumatology and Clinical Immunology, Freiburg; Rheumatology Clinical Practice Erlangen, Erlangen; Rheumatology Practice and Department of Internal Medicine 2, Clinic Burghausen, Burghausen; Asklepios Medical Center, Department of Rheumatology and clinical Immunology, Bad Abbach; Rheumatology Practice Bamberg, Bamberg; Rheumatology Practice Bayreuth, Bayreuth; University of Heidelberg, Medicine 5, Heidelberg; Rheumatology Practice Nuremberg, Nuremberg; Schlosspark Klinik, Internal Medicine/Rheumatology, Berlin; University of Tübingen, Centre for Interdisciplinary Clinical Immunology, Tübingen; Praxiszentrum St. Bonifatius, Munich, Germany. M. Hagen, BSc, CandMed, FAU, Department of Internal Medicine 3 - Rheumatology and Immunology, Universitätsklinikum Erlangen; M. Englbrecht, PhD, FAU, Department of Internal Medicine 3 - Rheumatology and Immunology, Universitätsklinikum Erlangen; J. Haschka, MD, St. Vincent Hospital, Vinforce Study Group, Medical University of Vienna; M. Reiser, FAU, Department of Internal Medicine 3 - Rheumatology and Immunology, Universitätsklinikum Erlangen; A. Kleyer, MD, FAU, Department of Internal Medicine 3 - Rheumatology and Immunology, Universitätsklinikum Erlangen; A. Hueber, MD, FAU, Department of Internal Medicine 3 - Rheumatology and Immunology, Universitätsklinikum Erlangen; B. Manger, MD, FAU, Department of Internal Medicine 3 - Rheumatology and Immunology, Universitätsklinikum Erlangen; C. Figueiredo, MD, Institutio de Rheumatologia; J. Fogagnolo Cobra, MD, Institutio de Rheumatologia; H.P. Tony, MD, University of Würzburg, Internal Medicine 2; S. Finzel, MD, University Medical Center Freiburg, Rheumatology and Clinical Immunology; S. Kleinert, MD, Rheumatology Clinical Practice Erlangen; J. Wendler, MD, Rheumatology Clinical Practice Erlangen; F. Schuch, MD, Rheumatology Clinical Practice Erlangen; M. Ronneberger, MD, Rheumatology Clinical Practice Erlangen; M. Feuchtenberger, MD, Rheumatology Practice and Department of Internal Medicine 2, Clinic Burghausen; M. Fleck, MD, Asklepios Medical Center, Department of Rheumatology and clinical Immunology; K. Manger, MD, Rheumatology Practice Bamberg; W. Ochs, MD, Rheumatology Practice Bayreuth; H.M. Lorenz, MD, University of Heidelberg, Medicine 5; H. Nüsslein, MD, Rheumatology Practice Nuremberg; R. Alten, MD, Schlosspark Klinik, Internal Medicine/Rheumatology; J. Henes, MD, University of Tübingen, Centre for Interdisciplinary Clinical Immunology; K. Krüger, MD, Praxiszentrum St. Bonifatius; G. Schett, MD, FAU, Department of Internal Medicine 3 - Rheumatology and Immunology, Universitätsklinikum Erlangen; J. Rech, MD, FAU, Department of Internal Medicine 3 - Rheumatology and Immunology, Universitätsklinikum Erlangen. Address correspondence to Dr. J. Rech, Friedrich-Alexander-University Erlangen-Nuremberg, Department of Internal Medicine 3 - Rheumatology and Immunology, Universitätsklinikum Erlangen, Ulmenweg 18, 91054 Erlangen, Germany. E-mail: Accepted for publication September 7, 2018.

Objective: To analyze the effect of a risk-stratified disease-modifying antirheumatic drug (DMARD)-tapering algorithm based on multibiomarker disease activity (MBDA) score and anticitrullinated protein antibodies (ACPA) on direct treatment costs for patients with rheumatoid arthritis (RA) in sustained remission.

Methods: The study was a posthoc retrospective analysis of direct treatment costs for 146 patients with RA in sustained remission tapering and stopping DMARD treatment, in the prospective randomized RETRO study. MBDA scores and ACPA status were determined in baseline samples of patients continuing DMARD (arm 1), tapering their dose by 50% (arm 2), or stopping after tapering (arm 3). Read More

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December 2018
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Effects of B-cell directed therapy on the preclinical stage of rheumatoid arthritis: the PRAIRI study.

Ann Rheum Dis 2018 Dec 1. Epub 2018 Dec 1.

Department of Clinical immunology and Rheumatology, Amsterdam Rheumatology and Immunology Center, Amsterdam University Medical Centre, Amsterdam, The Netherlands

Objectives: We explored the effects of B-cell directed therapy in subjects at risk of developing autoantibodypositive rheumatoid arthritis (RA), who never experienced inflammatory arthritis before, and explored biomarkers predictive of arthritis development.

Methods: Individuals positive for both anti-citrullinated peptide antibodies and rheumatoid factor but without arthritis were included in a randomised, double-blind, placebo-controlled study to receive a single infusion of 1000 mg rituximab or placebo.

Results: Eighty-one individuals received treatment and were followed up for a mean of 29. Read More

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December 2018
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Relationship Between Preoperative Erythrocyte Sedimentation Rate and Survival After Surgery in Patients with Colorectal Cancer.

Anticancer Res 2018 Dec;38(12):6783-6788

Department of Gastroenterological Surgery, Dokkyo Medical University, Tochigi, Japan.

Aim: To investigate the relationship between erythrocyte sedimentation rate (ESR) and postoperative survival of patients with colorectal cancer (CRC).

Materials And Methods: Relationships between clinical characteristics and overall survival (OS) of patients with CRC were investigated using multivariate analysis. Receiver operating characteristics curve analysis was performed to decide the ideal cut-off values of clinical characteristics to divide patients into two groups, which were then compared using a survival curve analysis. Read More

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December 2018

Complement-driven anemia: more than just paroxysmal nocturnal hemoglobinuria.

Hematology Am Soc Hematol Educ Program 2018 Nov;2018(1):371-376

Division of Hematology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD.

Atypical hemolytic uremic syndrome (aHUS); hemolysis, elevated liver function tests, and low platelets syndrome; and transplant-associated thrombotic microangiopathy are related conditions, in that many patients harbor germline heterozygous mutations in genes that regulate the alternative pathway of complement (APC). Penetrance is variable because development of clinically significant disease appears to require supervention of a process such as inflammation. Complement activation on the endothelial surfaces leads to endothelial damage, platelet consumption, microthrombi, and a mechanical hemolytic anemia with schistocytes. Read More

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November 2018

miR-181b regulates ER stress induced neuron death through targeting Heat Shock Protein A5 following intracerebral haemorrhage.

Immunol Lett 2018 Nov 29. Epub 2018 Nov 29.

Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160, China. Electronic address:

Endoplasmic reticulum (ER) stress acts as a protein folding and contributes to neuronal damage and neurological deterioration following intracerebral hemorrhage (ICH). Heat Shock Protein A5 (HSPA5) serves as an essential regulator of the endoplasmic reticulum (ER) stress response. However, the specific mechanism has not been will identified. Read More

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November 2018

Nano- and microscale mechanical properties of erythrocytes in hereditary spherocytosis.

J Biomech 2018 Nov 16. Epub 2018 Nov 16.

Research Institute for Applied Problems of Mathematics and Informatics, Belarusian State University, Nezavisimosti Ave., 4, 220030 Minsk, Belarus.

Hereditary spherocytosis (HS), an erythrocyte membranopathy, is a heterogeneous disease, even at the level of the erythrocyte population. The paper aims at studying the mechanical properties (the Young's modulus, median and RMS roughness of friction force maps; fractal dimension, lacunarity and spatial distribution parameters of lateral force maps) of the cell surface layer of the erythrocytes of two different morphologies (discocytes and spherocytes) in HS using atomic force microscopy. The results of spatial-spectral and fractal analysis showed that the mechanical property maps of the HS spherocyte surface were more structurally homogeneous compared to the maps of HS discocytes. Read More

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November 2018

Synthesis of new betulinic acid/betulin-derived dimers and hybrids with potent antimalarial and antiviral activities.

Bioorg Med Chem 2018 Nov 14. Epub 2018 Nov 14.

Organic Chemistry Chair I and Interdisciplinary Center for Molecular Materials (ICMM), Friedrich-Alexander University of Erlangen-Nürnberg, Nikolaus-Fiebiger-Straße 10, 91058 Erlangen, Germany. Electronic address:

Severe malaria and viral infections cause life-threatening diseases in millions of people worldwide every year. In search for effective bioactive hybrid molecules, which may possess improved properties compared to their parent compounds, a series of betulinic acid/betulin based dimer and hybrid compounds carrying ferrocene and/or artesunic acid moieties, was designed and, synthesized de novo. Furthermore, they were analyzed in vitro against malaria parasites (growth inhibition of 3D7-strain P. Read More

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November 2018

Cytochemical patterns of the peripheral blood cells in Chinese alligator (Alligator sinensis).

Tissue Cell 2018 Dec 29;55:71-76. Epub 2018 Oct 29.

Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, 241000, China. Electronic address:

The Chinese alligator (Alligator sinensis) is an endemic rare crocodilian species in China. In this study, we investigated the cytochemical patterns of peripheral blood cells in Chinese alligators for the first time by a range of cytochemical staining techniques including periodic acid-Schiff(PAS), sudan black B(SBB), peroxidase(POX), alkaline phosphatase(AKP), acid phosphatase(ACP), chloroacetic acid AS-D naphthol esterase(AS-D) and α-naphthol acetate esterase(α-NAE) staining. Erythrocytes were positive for PAS, and negative for all the other staining; heterophils were strongly positive for SBB, POX, ACP, AKP, AS-D and α-NAE, while weakly positive for PAS staining; eosinophils were strongly positive for PAS, POX, AKP, ACP and AS-D, and weakly positive for SBB and α-NAE staining; basophils were strongly positive for PAS, positive for POX, ACP, AKP and α-NAE, and weakly positive for AS-D, while negative for SBB staining; monocytes were weakly positive for PAS, ACP, AKP and α-NAE, while negative for SBB, POX and AS-D staining; lymphocytes were weakly positive for PAS and α-NAE, negative for all the other staining; thrombocytes were weakly positive for PAS, and negative for all the other staining. Read More

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December 2018
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Histograms of Complete Blood Counts in Dogs: Maximizing Diagnostic Information.

Top Companion Anim Med 2018 Dec 3;33(4):141-146. Epub 2018 Jun 3.

Companion Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.

Histograms, which are an integral part of the automated complete blood count, are now available through most of the automatic hematology analyzers used in veterinary clinical practice. Data concerning the size and number of blood cells are graphically presented in histograms, and their variations are also illustrated. Important information that is not apparent from numerical results are sometimes provided by histograms. Read More

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December 2018

Purification and characterizations of a new mannose specific lectin from Bangladeshi cultivated sword bean with antiproliferative activities against Ehrlich ascites carcinoma cells in vivo in mice.

Int J Biol Macromol 2018 Nov 28. Epub 2018 Nov 28.

Department of Pharmacy, Faculty of Biomedical Sciences, Khwaja Yunus Ali University, Sirajgonj, Bangladesh.

A new mannose specific sword bean seed lectin (designated as SBSL) has been purified from the seeds of Canavalia gladiata by affinity chromatographic technique with the molecular weight of 28 ± 1 kDa. The lectin is stable up to 60 °C and showed maximum haemagglutinating activity at pH 7.5. Read More

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November 2018

Assessing prognosis and prediction of treatment response in early rheumatoid arthritis: systematic reviews.

Health Technol Assess 2018 Nov;22(66):1-294

School of Health and Related Research (ScHARR), University of Sheffield, Sheffield, UK.

Background: Rheumatoid arthritis (RA) is a chronic, debilitating disease associated with reduced quality of life and substantial costs. It is unclear which tests and assessment tools allow the best assessment of prognosis in people with early RA and whether or not variables predict the response of patients to different drug treatments.

Objective: To systematically review evidence on the use of selected tests and assessment tools in patients with early RA (1) in the evaluation of a prognosis (review 1) and (2) as predictive markers of treatment response (review 2). Read More

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November 2018

[Relationship between Iron Metabolism and Platelet Parameters in Patients with Iron Deficiency Anemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2018 Dec;26(6):1738-1741

Department of Hematology,The First Affiliated Hospital,Harbin Medical University,Harbin 150000,Heilongjiang

Objective: To investigate the relationship between iron metabolism and thrombocytosis.

Methods: iron metabolism indexes , erythrocyte and platelet parameters of iron deficiency anemia patients with thrombocytosis were collected, the correlation of platelet parameters with iron metabolism indexes and erythrocyte parameters was analysed; the difference in erythrocyte,platelet and iron parameters between severe anemia group (hemoglobin<60 g / L) and mild and moderate anemia group (hemoglobin≥60 g / L) were compared; the changes of platelet count before and after treatment were observed.

Results: There was a significant negative correlation between serum iron and platelet count (r=-0. Read More

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December 2018

The Substantial Improvement of Amphotericin B Selective Toxicity upon Modification of Mycosamine with Bulky Substituents.

Med Chem 2018 Dec 2. Epub 2018 Dec 2.

Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdań sk University of Technology, 11/12 Narutowicza St., 80-233 Gdańsk. Poland.

Background It is assumed that the unfavorable selective toxicity of an antifungal drug Amphotericin B (AmB) can be improved upon chemical modification of the antibiotic molecule. Objective The aim of this study was verification of the hypothesis that introduction of bulky substituents at the amino sugar moiety of the antibiotic may result in diminishment of mammalian in vitro toxicity of thus prepared AmB derivatives. Methods Twenty eight derivatives of AmB were obtained upon chemical modification of the amino group of mycosamine residue. Read More

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December 2018

Essentiality of Plasmodium falciparum plasmepsin V.

PLoS One 2018 5;13(12):e0207621. Epub 2018 Dec 5.

Malaria Biochemistry Laboratory, The Francis Crick Institute, London, United Kingdom.

The malaria parasite replicates within erythrocytes. The pathogenesis of clinical malaria is in large part due to the capacity of the parasite to remodel its host cell. To do this, intraerythrocytic stages of Plasmodium falciparum export more than 300 proteins that dramatically alter the morphology of the infected erythrocyte as well as its mechanical and adhesive properties. Read More

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December 2018
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Pulmonary high-resolution computed tomography findings in patients with synovitis, acne, pustulosis, hyperostosis and osteitis syndrome.

PLoS One 2018 5;13(12):e0206858. Epub 2018 Dec 5.

Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Objective: To characterize the high-resolution computed tomography (HRCT) pulmonary abnormalities in patients with synovitis, acne, pustulosis, hyperostosis, and osteitis (SAPHO) syndrome.

Methods: Pulmonary HRCT images were reconstructed from whole-spine computed tomography (CT) images of 67 patients with SAPHO syndrome. HRCT images of 58 healthy controls were also obtained and reviewed. Read More

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December 2018

Routine Diagnostic Tests for Periprosthetic Joint Infection Demonstrate a High False-Negative Rate and Are Influenced by the Infecting Organism.

J Bone Joint Surg Am 2018 Dec;100(23):2057-2065

The Rothman Institute at Thomas Jefferson University, Philadelphia, Pennsylvania.

Background: Current guidelines recommend serum erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) as the first-line testing for evaluation of suspected periprosthetic joint infection, in addition to synovial white blood-cell (WBC) count and polymorphonuclear percentage. However, the sensitivity and other diagnostic measures of these tests using a standardized definition of periprosthetic joint infection and the influence of organisms on these inflammatory markers remain inadequately investigated.

Methods: A retrospective review of an institutional database of 549 periprosthetic joint infection cases and 653 aseptic total joint arthroplasty revisions was performed. Read More

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December 2018
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Is tranexamic acid safe and reliable during tibial intramedullary nailing?

Ulus Travma Acil Cerrahi Derg 2018 Nov;24(6):575-580

Department of Orthopedics and Traumatology, Health Sciences University Ümraniye and Training and Research Hospital, İstanbul-Turkey.

Background: The aim of this study was to determine if the use of tranexamic acid (TXA) during intramedullary reaming treatment for tibial diaphyseal fractures was safe, reduced blood loss, or affected cost effectiveness.

Methods: A total of 70 patients with a tibia diaphysis fracture were randomized into 2 groups and prospectively followed for data on blood loss, thrombosis, and fracture healing. Preoperative TXA was administered intravenously to Group A, and Group B served as the control group. Read More

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November 2018

Impact of ultrasound and conventional extraction techniques on bioactive compounds and biological activities of blue butterfly pea flower (Clitoria ternatea L.).

Ultrason Sonochem 2019 Mar 12;51:12-19. Epub 2018 Oct 12.

Beijing Advance Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China; Beijing Engineering and Technology Research Center of Food Additives, School of Food and Chemical Technology, Beijing Technology and Business University, Beijing 100048, China. Electronic address:

The present study was conducted to evaluate the influence of ultrasound on bioactive compounds and biological activities of blue butterfly pea flower (Clitoria ternatea L.). For this purpose, optimized conditions (temperature 50 °C, time 150 min, solid to liquid ratio 1 g:15 ml, 70% amplitude and 240 W, 20 kHz frequency, 3 s on and 3 s off) of ultrasound (US) and conventional extraction (AGE: Agitation, water bath for 150 min, 50 °C at 150 rmp) were used. Read More

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Accuracy of One Step malaria rapid diagnostic test (RDT) in detecting Plasmodium falciparum placental malaria infection in women living in Yaoundé, Cameroon.

Malar J 2018 Dec 4;17(1):450. Epub 2018 Dec 4.

The Biotechnology Center, University of Yaoundé I, P.O. Box 3851, Messa, Yaoundé, Cameroon.

Background: Plasmodium falciparum infected erythrocytes sequestering in placental tissue release Plasmodium lactate dehydrogenase (pLDH) and histidine-rich protein-II (HRP-II). These proteins can be detected in peripheral blood using monoclonal antibody-based rapid diagnostic tests (RDTs). Nevertheless, studies to evaluate the reliability of RDTs in detecting placental malaria compared with microscopy of placental tissue impression smear (PTIS) as the gold standard are scarce. Read More

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December 2018

ANNALS EXPRESS: Hematopoietic alterations in neonates with congenital diaphragmatic hernia receiving extracorporeal membrane oxygenation support.

Ann Clin Biochem 2018 Dec 4:4563218820052. Epub 2018 Dec 4.

Department of Neonatology and Pediatric Critical Care Medicine, University of Bonn Children's Hospital.

Background Extracorporeal membrane oxygenation procedure (ECMO) has been established in the therapy of respiratory insufficient infants with congenital diaphragmatic hernia (CDH). In CDH newborns, a delay in transfer to an ECMO center is associated with a sharp increase in mortality. Predictive factors for ECMO support are urgently needed. Read More

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December 2018

[Application of cortical bone trajectory screw in elderly patients with lumbar tuberculosis].

Zhongguo Gu Shang 2018 Nov;31(11):1012-1016

Department of Orthopaedics, Integrated Chinese and Western Medicine Hospital of Zhejiang Province, Hangzhou 310003, Zhejiang, China.

Objective: To explore the clinical effect of cortical bone trajectory screw technology combined with anterior mini-open debridement and prop graft for lumbar tuberculosis in elderly.

Methods: The clinical data of 22 patients with lumbar tuberculosis treated by cortical bone trajectory screw technology combined with anterior mini-open debridement and prop graft from February 2015 to December 2016 were retrospectively analyzed. There were 13 males and 9 females with an average age of (73. Read More

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November 2018
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[Treatment of senile spinal tuberculosis with posterior percutaneous pedicle screw fixation combined with local percutaneous endoscopic debridement].

Zhongguo Gu Shang 2018 Nov;31(11):1005-1011

Department of Surgery, Ningbo No.6 Hospital, Ningbo 315040, Zhejiang, China;

Objective: To explore the short-term efficacy of posterior percutaneous screw fixation combined with local percutaneous endoscopic debridement in treating senile spinal tuberculosis.

Methods: The clinical data of 19 senile patients with spinal tuberculosis underwent surgical treatment from January 2015 to September 2016 were retrospectively analyzed. There were 13 males and 6 females, aged from 60 to 73 years old with an average of (66. Read More

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November 2018

[Feasibility study of preoperative percutaneous catheter drainage in the treatment of lumbar tuberculosis with psoas abscess].

Zhongguo Gu Shang 2018 Nov;31(11):998-1004

Department of Orthopaedics, Hospital of Integrated Traditional Chinese and Western Medicine of Zhejiang Province, Hangzhou 310003, Zhejiang, China.

Objective: To investigate the effect of preoperative percutaneous drainage in lumbar tuberculosis with psoas abscess, through compare the clinical efficacy of two different methods.

Methods: Thirty-six patients with lumbar tuberculosis complicated with psoas abscess in corresponding with the inclusion criteria were admitted in Zhejiang Province Traditional Chinese Medicine Hospital from January 2015 to January 2017. The patients were divided into group A and group B according to the different therapeutic methods. Read More

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November 2018

[Treatment of tuberculosis of lumbar spine in elderly patients with percataneous endoscopic focal cleaning and drainage combined with posterior internal fixation].

Zhongguo Gu Shang 2018 Nov;31(11):987-992

Department of Orthopaedics, Hangzhou Red Cross Hospital, Hangzhou, 310003, Zhejiang, China.

Objective: To explore the clinical effects and advantages of percataneous endoscopic focal cleaning and drainage combined with posterior internal fixation in the treatment of tuberculosis of lumbar spine in elderly.

Methods: The clinical data of 32 patients with tuberculosis of lumbar spine received percataneous endoscopic focal cleaning and drainage combined with posterior internal fixation from May 2013 to May 2016 were retrospectively analyzed. There were 13 males and 19 females, aged from 62 to 85 years old with an average of 75 years. Read More

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November 2018
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Buccal Bullfrog ( Shaw) Oil Emulsion: A Mucoadhesive System Intended for Treatment of Oral Candidiasis.

Pharmaceutics 2018 Dec 3;10(4). Epub 2018 Dec 3.

Graduate Program in Health Sciences, Federal University of Rio Grande do Norte (UFRN), Av. General Gustavo Cordeiro de Faria-SN-Petrópolis, Natal 59012-570, Brazil.

Oral candidiasis (OC) is an infectious disease caused by microorganisms of the genus , leading to lesions in the buccal cavity. Its treatment consists of the administration of topical or systemic antifungal agents, which may compromise the patient compliance due to its side effects, highlighting the need for alternative treatments. In this scenario, bullfrog oil, an animal oil composed of a pool of saturated and unsaturated fatty acids, is introduced as a potential antifungal raw material. Read More

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December 2018

Modulation of Human Complement System by Antimicrobial Peptide Arenicin-1 from .

Mar Drugs 2018 Dec 1;16(12). Epub 2018 Dec 1.

Institute of Experimental Medicine, Acad. Pavlov Str. 12, Saint Petersburg 197376, Russia.

Antimicrobial peptides from marine invertebrates are known not only to act like cytotoxic agents, but they also can display some additional activities in mammalian organisms. In particular, these peptides can modulate the complement system as was described for tachyplesin, a peptide from the horseshoe crab. In this work, we investigated the influence on complement activation of the antimicrobial peptide arenicin-1 from the marine polychaete . Read More

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December 2018

Altered blood rheology and impaired pressure-induced cutaneous vasodilation in a mouse model of combined type 2 diabetes and sickle cell trait.

Microvasc Res 2018 Dec 1. Epub 2018 Dec 1.

UMR 5305 CNRS, Lyon, France; Université de Lyon 1, UMR 5305, Lyon, France.

Objective: Type 2 diabetes (T2D)-related vascular dysfunction and hemorheological abnormalities could possibly be amplified by sickle cell trait (SCT). These alterations could potentially increase the risk of vascular complications in individuals with combined T2D and SCT. Therefore, this study used a mouse model to determine whether vascular function and blood rheology were more severely altered in combined T2D and SCT than in T2D or SCT alone. Read More

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December 2018

Contribution of Plasmodium immunomics: potential impact for serological testing and surveillance of malaria.

Expert Rev Proteomics 2018 Dec 4. Epub 2018 Dec 4.

a Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health , National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, National Centre for International Research on Tropical Diseases, WHO Collaborating Center for Tropical Diseases , Shanghai 200025 , People's Republic of China.

Introduction: Plasmodium vivax and P. knowlesi account together for a considerable share of the global burden of malaria, along with P. falciparum. Read More

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December 2018
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