- Rita de Cássia Nasser Cubel Garcia,
- Renata Freire Alves Pereira,
- Kátia Martins Lopes de Azevedo,
- Tatiana Xavier de Castro,
- Francisco C A Mello,
- Sérgio Setubal,
- Marilda M Siqueira,
- David Brown,
- Solange Artimos de Oliveira
Braz J Infect Dis 2017 Jan - Feb;21(1):102-106. Epub 2016 Nov 30.
Universidade Federal Fluminense, Faculdade de Medicina, Programa de Pós Graduação em Ciências Médicas, Niterói, RJ, Brazil; Universidade Federal Fluminense, Faculdade de Medicina, Disciplina de Doenças Infecciosas e Parasitárias, Niterói, RJ, Brazil.
This study was conducted to provide information on the genetic diversity of human parvovirus B19 (B19V) circulating in the municipality of Niterói, Rio de Janeiro, Southeast Brazil during 1996-2006, a period with two distinct outbreaks of B19V infection: 1999-2000 and 2004-2005. A total of 27 sera from patients with erythema infectiosum and five sera from HIV-infected patients that tested positive for B19V DNA during the study period were analyzed. To genotype B19V strains, a semi-nested PCR for partial amplification of the capsid gene was performed and sequence analysis revealed that 31 sequences belonged to subgenotype 1a (G1a) of the main genotype 1 and one sequence was characterized as subgenotype 3b (G3b). Read More