27,628 results match your criteria Epigenetics & Chromatin [Journal]


CARM1 and Paraspeckles Regulate Pre-implantation Mouse Embryo Development.

Cell 2018 Dec;175(7):1902-1916.e13

Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3EG, UK. Electronic address:

Nuclear architecture has never been carefully examined during early mammalian development at the stages leading to establishment of the embryonic and extra-embryonic lineages. Heterogeneous activity of the methyltransferase CARM1 during these stages results in differential methylation of histone H3R26 to modulate establishment of these two lineages. Here we show that CARM1 accumulates in nuclear granules at the 2- to 4-cell stage transition in the mouse embryo, with the majority corresponding to paraspeckles. Read More

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December 2018

Special Issue: (Bio)Chemical Aspects of Epigenetics.

Authors:
Takayoshi Suzuki

Chem Rec 2018 Dec;18(12):1659

Graduate School of Medical Science, Kyoto Prefectural University of Medicine 1-5 Shimogamohangi-Cho, Sakyo-Ku, Kyoto, 606-0823, Japan.

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December 2018

Genetic variants in DNMT1 and the risk of cardiac autonomic neuropathy in females with Type 1 diabetes.

J Diabetes Investig 2018 Dec 11. Epub 2018 Dec 11.

Laboratório de Carboidratos e Radioimunoensaio LIM-18, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR. Av. Dr. Arnaldo, 455São Paulo, SP, BR.

Aims/introduction: Epigenetics participate in the pathogenesis of metabolic memory, a situation in which hyperglycemia exerts prolonged deleterious effects even after its normalization. We tested the hypothesis that genetic variants in an epigenetic gene could predispose to diabetes complications.

Material And Methods: We assessed the frequency of 5 single nucleotide polymorphisms (SNPs) in the gene encoding DNA methytransferase 1 (DNMT1; rs8112895, rs7254567, rs11085721, rs17291414, and rs10854076) and their associations with diabetic kidney disease, retinopathy, distal polyneuropathy and autonomic cardiovascular neuropathy (CAN) in 359 long-term Type 1 diabetes individuals. Read More

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December 2018

Programmed death ligand-1 is associated with tumor infiltrating lymphocytes and poorer survival in urothelial cell carcinoma of the bladder.

Cancer Sci 2018 Dec 12. Epub 2018 Dec 12.

Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen (Zhongshan) University, Guangzhou, China.

Drugs blocking programmed death ligand-1 (PD-L1) have shown unprecedented activity in metastatic and unresectable bladder cancer. The purpose of the present study was to investigate the expression, clinical significance and association of PD-L1 with tumor-infiltrating lymphocytes (TIL) in resectable urothelial cell carcinoma of the bladder (UCB). In this retrospective study, 248 UCB patients who received radical cystectomy or transurethral resection were examined. Read More

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December 2018

Associations of sex hormone-binding globulin and testosterone with genome-wide DNA methylation.

BMC Genet 2018 Dec 14;19(1):113. Epub 2018 Dec 14.

Population Health Sciences, Bristol Medical School, University of Bristol, Bristol, UK.

Background: Levels of sex hormone-binding globulin (SHBG) and the androgen testosterone have been associated with risk of diseases throughout the lifecourse. Although both SHBG and testosterone have been shown to be highly heritable, only a fraction of that heritability has been explained by genetic studies. Epigenetic modifications such as DNA methylation may explain some of the missing heritability and could potentially inform biological knowledge of endocrine disease mechanisms involved in development of later life disease. Read More

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December 2018

Deriving Neural Cells from Pluripotent Stem Cells for Nanotoxicity Testing.

Methods Mol Biol 2019 ;1894:57-72

College of Veterinary Medicine, Western University of Health Sciences, Pomona, CA, USA.

Stem cells are undifferentiated biological cells that can differentiate into all lineages under defined control condition. Stem cell neuronal differentiation can faithfully recapitulate stages of neural development and generate neuronal progenitors, mature neurons, and glial cells. Stem cell technology will largely allow for the replacement of animal studies and reduce costs, and will provide a new paradigm for in toxic genomics, bioinformatics, systems biology, and epigenetics studies. Read More

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January 2019

Treatment of metastatic non-small cell lung cancer with NY-ESO-1 specific TCR engineered-T cells in a phase I clinical trial: A case report.

Oncol Lett 2018 Dec 1;16(6):6998-7007. Epub 2018 Oct 1.

Department of Research and Development, Shenzhen Institute for Innovation and Translational Medicine, Shenzhen, Guangdong 518120, P.R. China.

This article presented a case of a human leukocyte antigen (HLA)-A2-positive patient with advanced cancer/testis antigen New York esophageal squamous cell carcinoma-1 (NY-ESO-1) expressing lung adenocarcinoma (LADC) who received adoptive cell therapy of T cell receptor engineered-T cells (TCR-T cells) targeting the cancer-testis antigen NY-ESO-1. The appropriate clinical and laboratory assessments were conducted to investigate the safety and efficacy of this therapy for this lung cancer patient. The patient had a clinical response to and was well-tolerated with this therapy in the clinical trial. Read More

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December 2018

Global long terminal repeat activation participates in establishing the unique gene expression programme of classical Hodgkin lymphoma.

Leukemia 2018 Dec 13. Epub 2018 Dec 13.

Institute for Cancer and Genomic Sciences, University of Birmingham, College of Medical and Dental Sciences, Birmingham, B152TT, UK.

Long terminal repeat (LTR) elements are wide-spread in the human genome and have the potential to act as promoters and enhancers. Their expression is therefore under tight epigenetic control. We previously reported in classical Hodgkin Lymphoma (cHL) that a member of the THE1B class of LTR elements acted as a promoter for the proto-oncogene and growth factor receptor gene CSF1R and that expression of this gene is required for cHL tumour survival. Read More

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December 2018

Analysis of differentially methylated regions in great apes and extinct hominids provides support for the evolutionary hypothesis of schizophrenia.

Schizophr Res 2018 Dec 10. Epub 2018 Dec 10.

NORMENT - K.G. Jebsen Center for Psychosis Research, Department of Clinical Science, University of Bergen, Bergen, Norway; Dr. Einar Martens Research Group for Biological Psychiatry, Department of Medical Genetics, Haukeland University Hospital, Bergen, Norway. Electronic address:

Introduction: The persistence of schizophrenia in human populations separated by geography and time led to the evolutionary hypothesis that proposes schizophrenia as a by-product of the higher cognitive abilities of modern humans. To explore this hypothesis, we used here an evolutionary epigenetics approach building on differentially methylated regions (DMRs) of the genome.

Methods: We implemented a polygenic enrichment testing pipeline using the summary statistics of genome-wide association studies (GWAS) of schizophrenia and 12 other phenotypes. Read More

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December 2018

Significantly altered peripheral blood cell DNA methylation profile as a result of immediate effect of metformin use in healthy individuals.

Clin Epigenetics 2018 Dec 13;10(1):156. Epub 2018 Dec 13.

Latvian Biomedical Research and Study Centre, Ratsupites Str. 1 k-1, Riga, LV-1067, Latvia.

Background: Metformin is a widely prescribed antihyperglycemic agent that has been also associated with multiple therapeutic effects in various diseases, including several types of malignancies. There is growing evidence regarding the contribution of the epigenetic mechanisms in reaching metformin's therapeutic goals; however, the effect of metformin on human cells in vivo is not comprehensively studied. The aim of our study was to examine metformin-induced alterations of DNA methylation profiles in white blood cells of healthy volunteers, employing a longitudinal study design. Read More

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December 2018
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Machine learning selected smoking-associated DNA methylation signatures that predict HIV prognosis and mortality.

Clin Epigenetics 2018 Dec 13;10(1):155. Epub 2018 Dec 13.

Department of Psychiatry, Yale School of Medicine, 300 George Street, 950 Campbell Ave, West Haven, New Haven, CT, 06511, USA.

Background: The effects of tobacco smoking on epigenome-wide methylation signatures in white blood cells (WBCs) collected from persons living with HIV may have important implications for their immune-related outcomes, including frailty and mortality. The application of a machine learning approach to the analysis of CpG methylation in the epigenome enables the selection of phenotypically relevant features from high-dimensional data. Using this approach, we now report that a set of smoking-associated DNA-methylated CpGs predicts HIV prognosis and mortality in an HIV-positive veteran population. Read More

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December 2018

The epigenetically downregulated factor CYGB suppresses breast cancer through inhibition of glucose metabolism.

J Exp Clin Cancer Res 2018 Dec 13;37(1):313. Epub 2018 Dec 13.

Chongqing Key Laboratory of Molecular Oncology and Epigenetics, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: Recent studies suggested the globin family member cytoglobin (CYGB) as a potential tumor suppressor; however, the mechanism by which CYGB suppresses cancer is elusive. We investigated the role and mechanism of CYGB in suppressing breast cancer.

Methods: CYGB expression was examined by reverse transcription PCR, quantitative reverse transcription PCR and open database analysis. Read More

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December 2018

Activity Landscape and Molecular Modeling to Explore the SAR of Dual Epigenetic Inhibitors: A Focus on G9a and DNMT1.

Molecules 2018 Dec 11;23(12). Epub 2018 Dec 11.

Department of Pharmacy, School of Chemistry, National Autonomous University of Mexico, 04510 Mexico City, Mexico.

In this work we discuss the insights from activity landscape, docking and molecular dynamics towards the understanding of the structure-activity relationships of dual inhibitors of major epigenetic targets: lysine methyltransferase (G9a) and DNA methyltranferase 1 (DNMT1). The study was based on a novel data set of 50 published compounds with reported experimental activity for both targets. The activity landscape analysis revealed the presence of activity cliffs, e. Read More

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December 2018

The Epigenetic Regulation of HCC Metastasis.

Int J Mol Sci 2018 Dec 10;19(12). Epub 2018 Dec 10.

Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.

Epigenetic alterations, such as histone modification, DNA methylation, and miRNA-mediated processes, are critically associated with various mechanisms of proliferation and metastasis in several types of cancer. To overcome the side effects and limited effectiveness of drugs for cancer treatment, there is a continuous need for the identification of more effective drug targets and the execution of mechanism of action (MOA) studies. Recently, epigenetic modifiers have been recognized as important therapeutic targets for hepatocellular carcinoma (HCC) based on their reported abilities to suppress HCC metastasis and proliferation in both in vivo and in vitro studies. Read More

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December 2018

Mechanisms of DNA Methyltransferase Recruitment in Mammals.

Genes (Basel) 2018 Dec 10;9(12). Epub 2018 Dec 10.

Epigenetics and Cell Fate, UMR7216 CNRS, University Paris Diderot, Sorbonne Paris Cité, 75013 Paris, France.

DNA methylation is an essential epigenetic mark in mammals. The proper distribution of this mark depends on accurate deposition and maintenance mechanisms, and underpins its functional role. This, in turn, depends on the precise recruitment and activation of de novo and maintenance DNA methyltransferases (DNMTs). Read More

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December 2018

miRNA Signature in NAFLD: A Turning Point for a Non-Invasive Diagnosis.

Int J Mol Sci 2018 Dec 10;19(12). Epub 2018 Dec 10.

General Medicine and Metabolic Diseases, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milano 20122, Italy.

Nonalcoholic fatty liver disease (NAFLD) defines a wide pathological spectrum ranging from simple steatosis to nonalcoholic steatohepatitis (NASH) which may predispose to liver cirrhosis and hepatocellular carcinoma. It represents the leading cause of hepatic damage worldwide. Diagnosis of NASH still requires liver biopsy but due to the high prevalence of NAFLD, this procedure, which is invasive, is not practicable for mass screening. Read More

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December 2018

Repetitive Fragile Sites: Centromere Satellite DNA As a Source of Genome Instability in Human Diseases.

Genes (Basel) 2018 Dec 7;9(12). Epub 2018 Dec 7.

Laboratory of Chromosome and Cell Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.

Maintenance of an intact genome is essential for cellular and organismal homeostasis. The centromere is a specialized chromosomal locus required for faithful genome inheritance at each round of cell division. Human centromeres are composed of large tandem arrays of repetitive alpha-satellite DNA, which are often sites of aberrant rearrangements that may lead to chromosome fusions and genetic abnormalities. Read More

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December 2018

The involvement of epigenetics in vascular disease development.

Int J Biochem Cell Biol 2018 Dec 10. Epub 2018 Dec 10.

Humanitas Research Hospital, Rozzano, Milan, Italy; Humanitas University, Rozzano, Milan, Italy. Electronic address:

Cardiovascular diseases are a major cause of death and disability. Despite enormous progress in diagnosis, prevention, and treatment over the years, the incidence of this group of pathologies continues to increase worldwide. An important step in reversing this situation is filling in the gaps we have in our understanding of cardiovascular homeostasis and of the pathogenic processes leading to disease. Read More

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December 2018

Ex Vivo Transposon-Mediated Genetic Screens for Cancer Gene Discovery.

Methods Mol Biol 2019 ;1907:145-157

Department of Molecular Biology, UT Southwestern Medical Center, Dallas, TX, USA.

Transposon mutagenesis has emerged as a powerful methodology for functionally annotating cancer genomes. Although in vivo transposon-mediated forward genetic screens have proven to be valuable for cancer gene identification, they are also time consuming and resource intensive. To facilitate the rapid and cost-effective identification of genes that regulate tumor-promoting pathways, we developed a complementary ex vivo transposon mutagenesis approach wherein human or mouse cells growing in culture are mutagenized and screened for the acquisition of specific phenotypes in vitro or in vivo, such as growth factor independence or tumor-forming ability. Read More

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January 2019

Epigenetic suppression of E-cadherin expression by Snail2 during the metastasis of colorectal cancer.

Clin Epigenetics 2018 Dec 12;10(1):154. Epub 2018 Dec 12.

National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin province, People's Republic of China.

Background: The transcription factor Snail2 is a repressor of E-cadherin expression during carcinogenesis; however, the specific mechanisms involved in this process in human colorectal cancer (CRC) remain largely unknown.

Method: We checked the expression of Snail2 in several clinical CRC specimens. Then, we established Snail2-overexpressing and knockdown cell lines to determine the function of Snail2 during EMT and metastasis processes in CRC. Read More

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December 2018

5-Hydroxytryptamine (5-HT)-exacerbated DSS-induced colitis is associated with elevated NADPH oxidase expression in the colon.

J Cell Biochem 2018 Dec 7. Epub 2018 Dec 7.

Department of Gastroenterology and Hepatology, The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China.

Aim: This study investigated the impact of 5-hydroxytryptamine (5-HT) on the expression of NOXs in dextran sulfate sodium (DSS)-induced colitis in mice.

Methods: C57BL/6J (B6) mice at 6 to 8 weeks of age were treated with 5-HT, DSS, or DSS + 5-HT. After 6-day treatment, the severity of colitis, infiltration of leukocytes, and messenger RNA (mRNA) and/or protein levels of Nox1, Nox2, Nox4, and Duox2 were analyzed in the colon by real-time quantitative polymerase chain reaction (qPCR), immunohistochemistry (IHC), and Western blot analysis. Read More

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December 2018

Epigenetic inhibitors for the precision treatment of -mutant ovarian cancers: what are the next steps?

Expert Rev Precis Med Drug Dev 2018 25;3(4):233-236. Epub 2018 Jul 25.

Department of Obstetrics & Gynecology, University of Colorado Anschutz Medical Campus, Research Complex-2; MS8613, 12700 East 19th Avenue, Aurora, Colorado 80045.

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Rapid Synthesis of -Tosylhydrazones under Solvent-Free Conditions and Their Potential Application Against Human Triple-Negative Breast Cancer.

ChemistryOpen 2018 Dec 21;7(12):977-983. Epub 2018 Nov 21.

College of Basic Medicine Jining Medical University Jining 272067 China.

Some -tosylhydrazone derivatives were effectively synthesized under solvent-free conditions by using a grinding method at room temperature. The short reaction time, clean and mild process with simple workup and easy purification of the target compounds were salient features of the present protocol, which enables straightforward access to -tosylhydrazones. Among the tosylhydrazone derivatives evaluated, compound exhibits excellent apoptosis-promoting and anticancer potential against triple-negative breast cancer (TNBC) cell lines. Read More

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December 2018

Metformin in Reproductive Biology.

Front Endocrinol (Lausanne) 2018 22;9:675. Epub 2018 Nov 22.

Unité de Physiologie de la Reproduction et des Comportements, Centre Val de Loire, Institut National de la Recherche Agronomique, UMR85, Nouzilly, France.

Initially produced in Europe in 1958, metformin is still one of the most widely prescribed drugs to treat type II diabetes and other comorbidities associated with insulin resistance. Metformin has been shown to improve fertility outcomes in females with insulin resistance associated with polycystic ovary syndrome (PCOS) and in obese males with reduced fertility. Metformin treatment reinstates menstrual cyclicity, decreases the incidence of cesareans, and limits the number of premature births. Read More

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November 2018

Molecular Pathology and Pharmacological Treatment of Autism Spectrum Disorder-Like Phenotypes Using Rodent Models.

Front Cell Neurosci 2018 20;12:422. Epub 2018 Nov 20.

Institute of Neuroscience, National Yang-Ming University, Taipei, Taiwan.

Autism spectrum disorder (ASD) is a heterogeneous neurodevelopmental disorder with a high prevalence rate. The core symptoms of ASD patients are impaired social communication and repetitive behavior. Genetic and environmental factors contribute to pathophysiology of ASD. Read More

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November 2018

Integrated comparison of the miRNAome and mRNAome in muscles of dermatomyositis and polymyositis reveals common and specific miRNA-mRNAs.

Epigenomics 2018 Dec 7. Epub 2018 Dec 7.

Department of Rheumatology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan 410008, PR China.

Aim: Dermatomyositis (DM) and polymyositis (PM) are refractory systemic autoimmune diseases with unknown pathogenesis. miRNAs is an important epigenetic mechanism to regulate gene expression.

Methods: We performed whole miRNAs analysis, transcription analysis and the association between miRNAome and mRNAome. Read More

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December 2018

Neurocognitive Impairment and Associated Genetic Aspects in HIV Infection.

Curr Top Behav Neurosci 2018 Dec 7. Epub 2018 Dec 7.

Department of Psychiatry, University of Alberta, Edmonton, AB, Canada.

HIV enters the central nervous system (CNS) early after infection. HIV-associated neurocognitive disorders (HAND) remain a serious complication of HIV infection despite available antiretroviral therapy (ART). Neurocognitive deficits observed in HAND are heterogeneous, suggesting a variability in individuals' susceptibility or resiliency to the detrimental CNS effects of HIV infection. Read More

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December 2018

Prognostic and Predictive Epigenetic Biomarkers in Oncology.

Mol Diagn Ther 2018 Dec 6. Epub 2018 Dec 6.

Molecular Oncology and Genetics Department, Innovative Medical Forum, The F. Lukaszczyk Oncology Center, Bydgoszcz, Poland.

Epigenetic patterns, such as DNA methylation, histone modifications, and non-coding RNAs, can be both driver factors and characteristic features of certain malignancies. Aberrant DNA methylation can lead to silencing of crucial tumor suppressor genes or upregulation of oncogene expression. Histone modifications and chromatin spatial organization, which affect transcription, regulation of gene expression, DNA repair, and replication, have been associated with multiple tumors. Read More

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December 2018

Interplay between exercise and BMI; results from an equal access, racially diverse biopsy study.

Cancer Causes Control 2018 Dec 6. Epub 2018 Dec 6.

Division of Urology, Veterans Affairs Medical Center, Durham, NC, USA.

Purpose: It is unclear if exercise and BMI interact to influence prostate cancer (PC) risk. We hypothesized BMI is linked with increased aggressive PC risk but this link will be attenuated with increased exercise.

Methods: Men undergoing prostate biopsy completed a questionnaire and metabolic equivalent (MET) hours of exercise was calculated. Read More

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December 2018
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The emerging link between cancer, metabolism, and circadian rhythms.

Nat Med 2018 Dec 6;24(12):1795-1803. Epub 2018 Dec 6.

Department of Biological Chemistry, Center for Epigenetics and Metabolism, INSERM U1233, University of California Irvine, Irvine, CA, USA.

The circadian clock is a complex cellular mechanism that, through the control of diverse metabolic and gene expression pathways, governs a large array of cyclic physiological processes. Epidemiological and clinical data reveal a connection between the disruption of circadian rhythms and cancer that is supported by recent preclinical data. In addition, results from animal models and molecular studies underscore emerging links between cancer metabolism and the circadian clock. Read More

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December 2018

A DNA methylation reader complex that enhances gene transcription.

Science 2018 12;362(6419):1182-1186

Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.

DNA methylation generally functions as a repressive transcriptional signal, but it is also known to activate gene expression. In either case, the downstream factors remain largely unknown. By using comparative interactomics, we isolated proteins in that associate with methylated DNA. Read More

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December 2018

Gestational diabetes mellitus alters DNA methylation profiles in pancreas of the offspring mice.

J Diabetes Complications 2018 Nov 9. Epub 2018 Nov 9.

Department of Endocrinology, Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian, China; Department of Scientific Research, Fujian Academy of Medical Sciences, Fuzhou, Fujian, China. Electronic address:

Gestational diabetes mellitus (GDM), which has an increasing global prevalence, contributes to the susceptibility to metabolic dysregulation and obesity in the offspring via epigenetic modifications. However, the underlying mechanism remains largely obscure. The current study established a GDM mice model to investigate the alternations in the metabolic phenotypes and genomic DNA methylation in the pancreas of the offspring. Read More

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November 2018

Physiological effects of KDM5C on neural crest migration and eye formation during vertebrate development.

Epigenetics Chromatin 2018 Dec 6;11(1):72. Epub 2018 Dec 6.

KNU-Center for Nonlinear Dynamics, CMRI, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, Daegu, 41566, South Korea.

Background: Lysine-specific histone demethylase 5C (KDM5C) belongs to the jumonji family of demethylases and is specific for the di- and tri-demethylation of lysine 4 residues on histone 3 (H3K4 me2/3). KDM5C is expressed in the brain and skeletal muscles of humans and is associated with various biologically significant processes. KDM5C is known to be associated with X-linked mental retardation and is also involved in the development of cancer. Read More

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December 2018

Correction to: Prediction of smoking by multiplex bisulfite PCR with long amplicons considering allele-specific effects on DNA methylation.

Clin Epigenetics 2018 Dec 6;10(1):151. Epub 2018 Dec 6.

Clinical Genetics Laboratory, Mental Health Research Center, Moscow, Russia.

Upon publication of the original article [1], it was noticed that the Figure captions of Figs. 2 and 3 were incorrectly given. The correct Figure captions are given below. Read More

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December 2018

EZH2 variants differentially regulate polycomb repressive complex 2 in histone methylation and cell differentiation.

Epigenetics Chromatin 2018 Dec 6;11(1):71. Epub 2018 Dec 6.

Department of Genetics, and Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599-7264, USA.

Background: Polycomb repressive complex 2 (PRC2) is responsible for establishing and maintaining histone H3K27 methylation during cell differentiation and proliferation. H3K27 can be mono-, di-, or trimethylated, resulting in differential gene regulation. However, it remains unknown how PRC2 specifies the degree and biological effects of H3K27 methylation within a given cellular context. Read More

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December 2018

Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming.

Cell Rep 2018 Dec;25(11):3059-3073.e10

Department of Biology, University of Rome Tor Vergata, Rome, Italy; IRCCS, Fondazione Santa Lucia, Rome, Italy. Electronic address:

Mitochondria are key players in the regulation of T cell biology by dynamically responding to cell needs, but how these dynamics integrate in T cells is still poorly understood. We show here that the mitochondrial pro-fission protein Drp1 fosters migration and expansion of developing thymocytes both in vitro and in vivo. In addition, we find that Drp1 sustains in vitro clonal expansion and cMyc-dependent metabolic reprogramming upon activation, also regulating effector T cell numbers in vivo. Read More

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December 2018
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Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring.

Neural Regen Res 2019 Mar;14(3):491-500

Programa de Pós-Graduação em Neurociências; Laboratório de Histofisiologia Comparada, Departamento de Ciências Morfológicas, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

It is widely known that maternal physical exercise is able to induce beneficial improvements in offspring cognition; however, the effects of paternal exercise have not been explored in detail. The present study was designed to evaluate the impact of paternal physical exercise on memory and learning, neuroplasticity and DNA methylation levels in the hippocampus of male offspring. Adult male Wistar rats were divided into two groups: sedentary or exercised fathers. Read More

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Enhancer of Zeste Homologue 2 Inhibition Attenuates TGF-β Dependent Hepatic Stellate Cell Activation and Liver Fibrosis.

Cell Mol Gastroenterol Hepatol 2018 Sep 15;7(1):197-209. Epub 2018 Sep 15.

Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota. Electronic address:

Background & Aims: Transdifferentiation of hepatic stellate cells (HSCs) into myofibroblasts is a key event in the pathogenesis of liver fibrosis. Transforming growth factor β (TGF-β) and platelet-derived growth factor (PDGF) are canonical HSC activators after liver injury. The aim of this study was to analyze the epigenetic modulators that differentially control TGF-β and PDGF signaling pathways. Read More

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September 2018

Detection and Quantification of Pseudouridine in RNA.

Methods Mol Biol 2019 ;1870:219-235

Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY, USA.

Pseudouridylation is the most abundant of all RNA modifications. Pseudouridylation is dynamic and widespread among many different types of RNAs in living organisms, thus drawing a lot of recent interest from the RNA and epigenetics communities. To successfully carry out an investigation into RNA pseudouridylation, it is desirable to have a convenient and effective method capable of detection and quantification of pseudouridylation. Read More

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January 2019

Identification of Methylated Transcripts Using the TRIBE Approach.

Methods Mol Biol 2019 ;1870:89-106

Laboratory of RNA Epigenetics, Institute of Molecular Biology (IMB), Mainz, Germany.

mA is the most abundant internal modification on mRNA. Recent improvements of high-throughput sequencing techniques enables its detection at the transcriptome level, even at the nucleotide resolution. However most current techniques require large amounts of starting material to detect the modification. Read More

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January 2019

The methylation and telomere landscape in two families of marsupials with different rates of chromosome evolution.

Chromosome Res 2018 Dec 12. Epub 2018 Dec 12.

Institute for Applied Ecology, University of Canberra, Canberra, ACT, 2617, Australia.

Two marsupial families exemplify divergent rates of karyotypic change. The Dasyurid family has an extremely conserved karyotype. In contrast, there is significant chromosomal variation within the Macropodidae family, best exemplified by members of the genus Petrogale (rock-wallabies). Read More

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December 2018

Invasive breast cancers in adolescent and young adult women show more aggressive immunohistochemical and clinical features than those in women aged 40-44 years.

Breast Cancer 2018 Dec 11. Epub 2018 Dec 11.

Department of Breast and Medical Oncology, National Cancer Center Hospital, Tokyo 5-1-1 Tsukiji, Chuo-ku, Tokyo, 104-0045, Japan.

Background: Limited knowledge exists concerning the clinicopathological features of breast cancers (BCs) occurring in adolescent and young adult (AYA) women. We evaluated tumor characteristics in AYA women in comparison with those in middle-aged premenopausal women.

Methods: From consecutive AYA patients (< 35-year-old) with invasive BC in a single institute, 82 patients first treated with surgery were examined. Read More

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December 2018

FOXG1 Regulates PRKAR2B Transcriptionally and Posttranscriptionally via miR200 in the Adult Hippocampus.

Mol Neurobiol 2018 Dec 11. Epub 2018 Dec 11.

Institute of Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine, Albert-Ludwigs-University Freiburg, 79104, Freiburg, Germany.

Rett syndrome is a complex neurodevelopmental disorder that is mainly caused by mutations in MECP2. However, mutations in FOXG1 cause a less frequent form of atypical Rett syndrome, called FOXG1 syndrome. FOXG1 is a key transcription factor crucial for forebrain development, where it maintains the balance between progenitor proliferation and neuronal differentiation. Read More

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December 2018

Plant natural modulators in breast cancer prevention: status quo and future perspectives reinforced by predictive, preventive, and personalized medical approach.

EPMA J 2018 Dec 12;9(4):403-419. Epub 2018 Nov 12.

1Department of Obstetrics and Gynecology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovakia.

In contrast to the genetic component in mammary carcinogenesis, epigenetic alterations are particularly important for the development of sporadic breast cancer (BC) comprising over 90% of all BC cases worldwide. Most of the DNA methylation processes are physiological and essential for human cellular and tissue homeostasis, playing an important role in a number of key mechanisms. However, if dysregulated, DNA methylation contributes to pathological processes such as cancer development and progression. Read More

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December 2018

Histone demethylase LSD1 is required for germinal center formation and BCL6-driven lymphomagenesis.

Nat Immunol 2019 Jan 10;20(1):86-96. Epub 2018 Dec 10.

Department of Medicine, Division of Hematology & Medical Oncology, Weill Cornell Medicine, New York, NY, USA.

Germinal center (GC) B cells feature repression of many gene enhancers to establish their characteristic transcriptome. Here we show that conditional deletion of Lsd1 in GCs significantly impaired GC formation, associated with failure to repress immune synapse genes linked to GC exit, which are also direct targets of the transcriptional repressor BCL6. We found that BCL6 directly binds LSD1 and recruits it primarily to intergenic and intronic enhancers. Read More

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January 2019

Myocardial infarction cell by cell.

Nat Immunol 2019 Jan;20(1):7-9

Department for Genomics & Immunoregulation, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany.

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January 2019

Publisher Correction: Tracing the transitions from pluripotency to germ cell fate with CRISPR screening.

Nat Commun 2018 12 11;9(1):5328. Epub 2018 Dec 11.

Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.

Ufuk Günesdogan was incorrectly associated with Center for Genetic Analysis of Behaviour, National Institute for Physiological Sciences, 5-1 Higashiyama Myodaiji, Okazaki, Aichi 444-8787, Japan and Toshihiro Kobayashi was incorrectly associated with Department of Developmental Biology, University of Göttingen, Göttingen Center for Molecular Biosciences, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany. This has now been corrected in both the PDF and HTML versions of the Article. Read More

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December 2018

Social status alters chromatin accessibility and the gene regulatory response to glucocorticoid stimulation in rhesus macaques.

Proc Natl Acad Sci U S A 2018 Dec 11. Epub 2018 Dec 11.

Department of Evolutionary Anthropology, Duke University, Durham, NC 27708;

Low social status is an important predictor of disease susceptibility and mortality risk in humans and other social mammals. These effects are thought to stem in part from dysregulation of the glucocorticoid (GC)-mediated stress response. However, the molecular mechanisms that connect low social status and GC dysregulation to downstream health outcomes remain elusive. Read More

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December 2018

Tet2-dependent hydroxymethylome plasticity reduces melanoma initiation and progression.

Cancer Res 2018 Dec 11. Epub 2018 Dec 11.

Laboratory of Cancer Epigenetics, Free University of Brussels

Although numerous epigenetic aberrancies accumulate in melanoma, their contribution to initiation and progression remain unclear. The epigenetic mark 5-hydroxymethylcytosine (5hmC), generated through TET-mediated DNA modification, is now referred to as the sixth base of DNA and has recently been reported as a potential biomarker for multiple types of cancer. Loss of 5hmC is an epigenetic hallmark of melanoma, but whether a decrease in 5hmc levels contributes directly to pathogenesis or whether it merely results from disease-progression-associated epigenetic remodelling remains to be established. Read More

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December 2018

HDAC is indispensable for IFN-γ-induced B7-H1 expression in gastric cancer.

Clin Epigenetics 2018 Dec 11;10(1):153. Epub 2018 Dec 11.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Background: B7 homolog 1 (B7-H1) overexpression on tumor cells is an important mechanism of immune evasion in gastric cancer (GC). Elucidation of the regulation of B7-H1 expression is urgently required to guide B7-H1-targeted cancer therapy. Interferon gamma (IFN-γ) is thought to be the main driving force behind B7-H1 expression, and epigenetic factors including histone acetylation are recently linked to the process. Read More

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December 2018
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