1,841 results match your criteria Enzyme and Microbial Technology [Journal]


Structure and catalytic mechanistic insight into Enterobacter aerogenes acetolactate decarboxylase.

Enzyme Microb Technol 2019 Jul 5;126:9-17. Epub 2019 Mar 5.

Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning, 116023, PR China. Electronic address:

α-Acetolactate decarboxylase (ALDC) catalyses α-acetolactate into acetoin (3-hydroxy-2-butanone, AC) and is considered to be the rate-limiting enzyme in the synthesis of 2,3-butanediol. In this work, the enzymatic activity of ALDC from Enterobacter aerogenes ALDC (E.a. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183063
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http://dx.doi.org/10.1016/j.enzmictec.2019.03.005DOI Listing
July 2019
1 Read

Recombinant expression and molecular insights into the catalytic mechanism of an NADPH-dependent conjugated polyketone reductase for the asymmetric synthesis of (R)-pantolactone.

Enzyme Microb Technol 2019 Jul 2;126:77-85. Epub 2019 Apr 2.

Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, 310032, PR China. Electronic address:

(R)-pantolactone is a key chiral intermediate for synthesizing calcium (R)-pantothenate. The commercial synthesis of (R)-pantolactone is performed through the resolution of racemic pantolactone using lactonase-catalyzed enantioselective hydrolysis. The process needs highly toxic hydrogen cyanide and a tedious dynamic kinetic resolution. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229193004
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http://dx.doi.org/10.1016/j.enzmictec.2019.04.001DOI Listing
July 2019
1 Read

Fusion of the N-terminal domain of Pseudomonas sp. phytase with Bacillus sp. phytase and its effects on optimal temperature and catalytic efficiency.

Enzyme Microb Technol 2019 Jul 3;126:69-76. Epub 2019 Apr 3.

Department of Biotechnology, Pukyong National University, Busan, 48513, Republic of Korea. Electronic address:

The beta-propeller phytase (BPP) is an enzyme that hydrolyzes phyate to release inorganic phosphorus. The BPP produced by Pseudomonas sp. FB15 (PSphy) possesses an additional N-terminal domain that is not present in BPP produced by other Bacillus species. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.04.002DOI Listing

Effects of divalent copper on microbial community, enzymatic activity and functional genes associated with nitrification and denitrification at tetracycline stress.

Enzyme Microb Technol 2019 Jul 25;126:62-68. Epub 2019 Mar 25.

College of Forestry, Northeast Forestry University, Harbin, China.

The effects of divalent copper (Cu(II)) on microbial community, enzymatic activity and functional genes in a sequencing batch reactor (SBR) at tetracycline (TC) stress were investigated. The enzymatic activity and functional genes abundance associated with nitrification and denitrification at a 20 mg L TC stress were higher than those at a mixtures stress of 20 mg L TC and 10 mg L Cu(II), while they were lower than those at a mixtures stress of 20 mg L TC and 40 mg L Cu(II). Compared to lactate dehydrogenase (LDH) release and reactive oxygen species (ROS) production at a 20 mg L TC stress, they were lower at the TC stress with 10 mg L Cu(II), while they were higher at the TC stress with 40 mg L Cu(II). Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.03.007DOI Listing
July 2019
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A thermostable chitinase from the antagonistic Chromobacterium violaceum that inhibits the development of phytopathogenic fungi.

Enzyme Microb Technol 2019 Jul 1;126:50-61. Epub 2019 Apr 1.

Laboratório de Genética Molecular, Departamento de Biologia, Centro de Ciências, UFC, Fortaleza, CE, Brazil. Electronic address:

The biocontrol activity of some soil strains of Chromobacterium sp. against pathogenic fungi has been attributed to secreted chitinases. The aim of this work was to characterize biochemically a recombinant chitinase (CvChi47) from C. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.03.009DOI Listing

Enhancing thermostability of Yarrowia lipolytica lipase 2 through engineering multiple disulfide bonds and mitigating reduced lipase production associated with disulfide bonds.

Enzyme Microb Technol 2019 Jul 30;126:41-49. Epub 2019 Mar 30.

College of Animal Science, South China Agricultural University, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.

The limited thermostability of Yarrowia lipolytica lipase 2 (Lip2) hampers its industrial application. To improve its thermostability, we combined single disulfide bonds which our group identified previously. In this study, combining different regional disulfide bonds had greater effect than combining same regional disulfide bonds. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.03.008DOI Listing
July 2019
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Identification of a novel carboxypeptidase encoded by Rv3627c that plays a potential role in mycobacteria morphology and cell division.

Enzyme Microb Technol 2019 Jul 4;126:32-40. Epub 2019 Mar 4.

Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian, 116044, China. Electronic address:

Functionally uncharacterized gene Rv3627c is predicted to encode a carboxypeptidase in the pathogen of Mycobacterium tuberculosis (M. tuberculosis), which remains a major threat to human health. Here, we sought to reveal the function of Rv3627c and to elucidate its effects on mycobacterial growth. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.03.003DOI Listing

Cells-on-nanofibers: Effect of polyethyleneimine on hydrophobicity of poly-Ɛ-caprolacton electrospun nanofibers and immobilization of bacteria.

Enzyme Microb Technol 2019 Jul 2;126:24-31. Epub 2019 Mar 2.

Ege University Faculty of Science Biochemistry Department, 35100 Bornova-Izmir, Turkey. Electronic address:

Among other synthetic polymers, poly-Ɛ-caprolacton (PCL) nanofibers are one of the most popular ones, especially in tissue engineering application due to its distinct mechanical and chemical properties. However, in some cases, lacking functional group on polymer structure obstructs the covalent modification of the PCL nanofibers for the aim. Herein, polyethyleneimine (PEI) was blended with PCL polymer to provide functional amino groups on the surface of the nanofiber mat. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.03.002DOI Listing

Enhanced lipase-catalyzed synthesis of sugar fatty acid esters using supersaturated sugar solution in ionic liquids.

Enzyme Microb Technol 2019 Jul 4;126:18-23. Epub 2019 Mar 4.

Department of Biological Engineering, Inha University, Incheon, 22212, Republic of Korea. Electronic address:

A solvent-mediated method (SMM) was used to prepare supersaturated sugar solutions in hydrophobic and mixture of hydrophilic/hydrophobic ionic liquids (ILs), namely, [Bmim][TfN] and [Bmim][TfO]/[Bmim][TfN], respectively. In this method, sugars were first solubilized in a mixture of organic solvent and water (i.e. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.03.004DOI Listing

High efficiency biotransformation of bisphenol A in a fluidized bed reactor using stabilized laccase in porous silica.

Enzyme Microb Technol 2019 Jul 28;126:1-8. Epub 2019 Mar 28.

Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun 130021, China. Electronic address:

We developed a high efficiency bisphenol A (BPA) treating system in a fluidized bed reactor (FBR) using stabilized laccase (Lac) in mesoporous silica (SER/Lac). The SER/Lac, prepared by cross linking of each enzymes inside the porous silica using glutaraldehyde, presented improved stability than the free Lac over various pH and temperatures with shaking. When the SER/Lac was used for BPA biotransformation in a batch reaction, higher efficiency was achieved than the free or adsorbed Lac (ADS/Lac). Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.03.006DOI Listing
July 2019
1 Read

Site-directed mutation to improve the enzymatic activity of 5-carboxy-2-pentenoyl-CoA reductase for enhancing adipic acid biosynthesis.

Enzyme Microb Technol 2019 Jun 16;125:6-12. Epub 2019 Feb 16.

National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, China. Electronic address:

Adipate is a linear C6 dicarboxylic acid, and is a crucial commercial material mainly used to produce the polymer nylon-6,6. In this study, the pathway producing adipate via a reverse reaction of degradation pathway of adipic acid was ported from Thermobifida fusca to Escherichia coli (E. coli). Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.02.006DOI Listing

Engineered thermostable β-fructosidase from Thermotoga maritima with enhanced fructooligosaccharides synthesis.

Enzyme Microb Technol 2019 Jun 6;125:53-62. Epub 2019 Feb 6.

Grupo Tecnología de Enzimas, Dirección de Investigaciones Agropecuarias, Centro de Ingeniería Genética y Biotecnología (CIGB), Ave 31 entre 158 y 190, Apartado Postal 6162, Habana, 10600, Cuba.

The thermostable β-fructosidase (BfrA) from the bacterium Thermotoga maritima converts sucrose into glucose, fructose, and low levels of short-chain fructooligosaccharides (FOS) at high substrate concentration (1.75 M) and elevated temperatures (60-70 °C). In this research, FOS produced by BfrA were characterized by HPAE-PAD analysis as a mixture of 1-kestotriose, 6-kestotriose (neokestose), and to a major extent 6-kestotriose. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.02.002DOI Listing

Strategy and regulatory mechanisms of glutamate feeding to enhance astaxanthin yield in Xanthophyllomyces dendrorhous.

Enzyme Microb Technol 2019 Jun 28;125:45-52. Epub 2019 Feb 28.

Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, PR China. Electronic address:

Xanthophyllomyces dendrorhous is an excellent industrial source for production of natural astaxanthin, but the yield of astaxanthin is relative low due to the contradiction between biomass weight and astaxanthin accumulation. Glutamate, a metabolite connecting nitrogen and carbon metabolisms, is probably a promising entry point to interfere cellular metabolisms. Thus, the effect of glutamate on cell growth and astaxanthin accumulation in X. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.02.010DOI Listing

A novel d-2-hydroxy acid dehydrogenase with high substrate preference for phenylpyruvate originating from lactic acid bacteria: Structural analysis on the substrate specificity.

Enzyme Microb Technol 2019 Jun 23;125:37-44. Epub 2019 Feb 23.

Department of Biochemical Engineering, Gangneung-Wonju National University, 7, Jukheon-gil, Gangneung-si, Gangwon-do, 25457, Republic of Korea. Electronic address:

2-Hydroxy acid dehydrogenases (2-HADHs) have been implicated in the synthesis of 2-hydroxy acids from 2-oxo acids that are used in wide areas of industry. d-lactate dehydrogenases (d-LDHs), a subfamily of 2-HADH, have been utilized to this purpose, yet they exhibited relatively low catalytic activity to the 2-oxo acids with large functional groups at C. In this report, four putative 2-HADHs from Oenococcus oeni, Weissella confusa, Weissella koreensis and Pediococcus claussenii were examined for activity on phenylpyruvate (PPA), a substrate to 3-phenyllactic acid (PLA) with a C phenyl group. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.02.008DOI Listing

Structure-guided engineering of ChKRED20 from Chryseobacterium sp. CA49 for asymmetric reduction of aryl ketoesters.

Enzyme Microb Technol 2019 Jun 4;125:29-36. Epub 2019 Mar 4.

Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu, 610041, China. Electronic address:

ChKRED20 is a robust NADH-dependent ketoreductase identified from the genome of Chryseobacterium sp. CA49 that can use 2-propanol as the ultimate reducing agent. The wild-type can reduce over 100 g/l ketones for some pharmaceutical relevant substrates, exhibiting a remarkable potential for industrial application. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.03.001DOI Listing

Manipulating intradiol dioxygenases by C-terminus truncation.

Enzyme Microb Technol 2019 Jun 19;125:21-28. Epub 2019 Feb 19.

Biotransformation, Scion, Rotorua, New Zealand. Electronic address:

Intradiol dioxygenases (EC 1.13.11. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.02.007DOI Listing

Carbohydrate-binding property of a cell wall integrity and stress response component (WSC) domain of an alcohol oxidase from the rice blast pathogen Pyricularia oryzae.

Enzyme Microb Technol 2019 Jun 25;125:13-20. Epub 2019 Feb 25.

Research Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizugawa, Kyoto 619-0292, Japan; Division of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0101, Japan. Electronic address:

The cell wall integrity and stress response component (WSC) domain was first described in the Wsc-family protein of the yeast Saccharomyces cerevisiae, and later found in diverse eukaryotic organisms. Due solely to their presence in the Wsc-family proteins working as a plasma membrane sensor for surface stress and in a fungal β-1,3-exoglucanse, WSC domains have been presumed to possess carbohydrate-binding property without any experimental evidence. Aiming at elucidation of function(s) of WSC domains, we characterized a WSC domain-containing alcohol oxidase from the rice blast pathogen Pyricularia oryzae (PoAlcOX). Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.02.009DOI Listing

Significantly improved catalytic efficiency of caffeic acid O-methyltransferase towards N-acetylserotonin by strengthening its interactions with the unnatural substrate's terminal structure.

Enzyme Microb Technol 2019 Jun 14;125:1-5. Epub 2019 Feb 14.

Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China. Electronic address:

O-Methylation of N-acetylserotonin (NAS) has been identified as the bottleneck in melatonin biosynthesis pathway. In the present paper, caffeic acid O-methyltransferase from Arabidopsis thaliana (AtCOMT) was engineered by rational design to improve its catalytic efficiency in conversion of NAS to melatonin. Based on the notable difference in the terminal structure of caffeic acid and NAS, mutants were designed to strengthen the interactions between the substrate binding pocket of the enzyme and the terminal structure of the unnatural substrate NAS. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.02.005DOI Listing

Effects of lipopolysaccharide structure on lycopene production in Escherichia coli.

Enzyme Microb Technol 2019 May 24;124:9-16. Epub 2019 Jan 24.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, China; Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China. Electronic address:

Lipopolysaccharides, the major molecules in the outer membrane of Escherichia coli, affect the behavior of bacteria including outer membrane permeability, but its influence on lycopene production in E. coli has never been reported. In this study, the effects of lipopolysaccharides with different structures on lycopene biosynthesis were investigated. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.009DOI Listing
May 2019
1 Read

Corrigendum to "N-terminal domain of the beta-propeller phytase of Pseudomonas sp. FB15 plays a role for retention of low-temperature activity and catalytic efficiency" [Enzyme Microb. Technol. 117 (October) (2018) 84-90].

Enzyme Microb Technol 2019 May 10;124:84. Epub 2019 Feb 10.

Department of Biotechnology, Pukyong National University, Busan, 48513, Republic of Korea. Electronic address:

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.013DOI Listing
May 2019
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Hydrogen-bond-based protein engineering for the acidic adaptation of Bacillus acidopullulyticus pullulanase.

Enzyme Microb Technol 2019 May 30;124:79-83. Epub 2019 Jan 30.

College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu, 241000, China. Electronic address:

Pullulanase is a starch-debranching enzyme that is generally employed to efficiently break down starch for the production of high-glucose syrup. Acidic adaptation of pullulanases is of special interest. In this study, we conducted protein engineering to improve the acidic adaptation of Bacillus acidopullulyticus pullulanase (BaPul) and used a hydrogen-bond-based approach to identify promising residues that may change the deprotonation constants (pK) of the catalytic residues. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.010DOI Listing
May 2019
9 Reads

Cloning and expression of a β-mannanase gene from Bacillus sp. MK-2 and its directed evolution by random mutagenesis.

Enzyme Microb Technol 2019 May 7;124:70-78. Epub 2019 Feb 7.

Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Electronic address:

A β-mannanase gene was cloned from Bacillus sp. MK-2 and expressed in Bacillus subtilis WB800. The ORF of the β-mannanase gene was 1104 bp in length, encoding 367 aa. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.02.003DOI Listing

Effect of intense pulsed light on the deactivation of lipase: Enzyme-deactivation kinetics and tertiary structural changes by fragmentation.

Enzyme Microb Technol 2019 May 5;124:63-69. Epub 2019 Feb 5.

Department of Agricultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea; Center for Food and Bioconvergence, Seoul National University, Seoul, 08826, Republic of Korea; Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea. Electronic address:

The effect of intense pulsed light (IPL) irradiation on Chromobacterium viscosum lipase was investigated with a primary focus on catalytic activity and molecular structure. During IPL irradiation, lipase activity decreased significantly with increasing pulse fluence (F) and exposure time (t). IPL-induced deactivation kinetics were further elucidated based on a two-step series-type deactivation model (constant deactivation rate k >k). Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.02.001DOI Listing
May 2019
1 Read

Molecular engineering of chitinase from Bacillus sp. DAU101 for enzymatic production of chitooligosaccharides.

Enzyme Microb Technol 2019 May 30;124:54-62. Epub 2019 Jan 30.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China. Electronic address:

Enzymatic production of chitooligosaccharides has high value in medicine and other fields. However, low chitinase activity and yield of chitooligosaccharides limit the production and application. Herein, we used a series of molecular biology strategies to increase the expression of chitinase in Bacillus subtilis WB600. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.012DOI Listing

Biochemical characterization of peptidylarginine deiminase-like orthologs from thermotolerant Emericella dentata and Aspergillus nidulans.

Enzyme Microb Technol 2019 May 7;124:41-53. Epub 2019 Feb 7.

Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.

Peptidylarginine deiminases (PADs) are a group of hydrolases, mediating the deimination of peptidylarginine residues into peptidyl-citrulline. Equivocal protein citrullination by PADs of fungal pathogens has a strong relation to the progression of multiple human diseases, however, the biochemical properties of fungal PADs remain ambiguous. Thus, this is the first report exploring the molecular properties of PAD from thermotolerant fungi, to imitate the human temperature. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.02.004DOI Listing
May 2019
3 Reads

Identification of enzyme(s) capable of degrading endosulfan and endosulfan sulfate using in silico techniques.

Enzyme Microb Technol 2019 May 4;124:32-40. Epub 2019 Jan 4.

Environmental Toxicology and Soil Microbial Ecology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007, India.

Endosulfan is one of the most widely used organochlorine cyclodiene insecticides. Microbial oxidation of endosulfan forms endosulfan sulfate, which is more or less toxic and persistent as endosulfan. Due to lack of specificity and efficiency of microbial bioremediation technique in the field conditions, enzymatic bioremediation is receiving huge attention to clean-up the environment. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.003DOI Listing
May 2019
1 Read

The modular arabinanolytic enzyme Abf43A-Abf43B-Abf43C from Ruminiclostridium josui consists of three GH43 modules classified in different subfamilies.

Enzyme Microb Technol 2019 May 30;124:23-31. Epub 2019 Jan 30.

Graduate School of Bioresources, Mie University, Tsu, 514-8507, Japan. Electronic address:

The abnA gene from Ruminiclostridium josui encodes the large modular arabinanolytic enzyme, Abf43A-Abf43B-Abf43C, consisting of an N-terminal signal peptide, a Laminin_G_3 module, a GH43_22 module, a Laminin_G_3 module, a Big_4 module, a GH43_26 module, a GH43_34 module and a dockerin module in order with a calculated molecular weight of 204,108. Three truncated enzymes were recombinantly produced in Escherichia coli and biochemically characterized, RjAbf43A consisting of the first Laminin_G_3 module and GH43_22 module, RjAbf43B consisting of the second Laminin_G_3 module, Big_4 module and GH43_26 module, and RjAbf43C consisting of the GH43_34 module. RjAbf43A showed a strong α-l-arabinofuranosidase activity toward sugar beet arabinan, highly branched arabinan but not linear arabinan, thus it acted in the removal of arabinose side chains from sugar beet arabinan. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.011DOI Listing
May 2019
2 Reads

Producing alcohol and salt stress tolerant strain of Saccharomyces cerevisiae by heterologous expression of pprI gene.

Enzyme Microb Technol 2019 May 23;124:17-22. Epub 2019 Jan 23.

Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran.

Introduction: Ethanol is considered a comparatively clean biofuel, and its large scale production has been a long time concern. Saccharomyces cerevisiae has proven to be the suitable microorganism for large scale ethanol production, but production of other alcohols like butanol and using lignocellulosic substrates is restricted due to lacking tolerance toward toxicity of alcohols, and compounds released from substrates. This study aimed to produce a tolerant strain by using pprI gene of Deinococcus radiodurans. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.008DOI Listing
May 2019
2 Reads

Xerogel based catalyst for improved cathode performance in microbial fuel cells.

Enzyme Microb Technol 2019 May 17;124:1-8. Epub 2019 Jan 17.

Department of Biotechnology, Indian Institute of Technology Madras, Chennai, 600 036, India. Electronic address:

In a microbial fuel cell (MFC) the reduction reaction at cathode has been a limiting factor in achieving maximum power density, and numerous strategies have been implemented in an attempt to overcome this. Herein, we demonstrate that carbon xerogel (CX) doped with iron (Fe) and nitrogen (N) followed by modification with graphene oxide (GO) is an efficient catalyst for MFCs. The CXFeNGO catalyst was characterized using a scanning electron microscope, and X-ray diffraction, and the catalytic activity was confirmed using cyclic voltammetry studies. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.007DOI Listing
May 2019
1 Read

Comparison of two models of surface display of xylose reductase in the Saccharomyces cerevisiae cell wall.

Enzyme Microb Technol 2019 Apr 6;123:8-14. Epub 2019 Jan 6.

Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10 000 Zagreb, Croatia. Electronic address:

In order to display xylose reductase at the surface of S. cerevisiae cells two different gene constructs have been prepared. In the first, xylose reductase gene GRE3 was fused with two parts of the CCW12 gene, the N-terminal one coding for the secretion signal sequence, and the C-terminal coding for the glycosylphosphatidylinositol anchoring signal. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.005DOI Listing

An improved enzyme nanoparticles based amperometric pyruvate biosensor for detection of pyruvate in serum.

Enzyme Microb Technol 2019 Apr 11;123:30-38. Epub 2019 Jan 11.

Department of Biochemistry, M.D. University, Rohtak, Haryana, India. Electronic address:

The nanoparticles of commercially available pyruvate oxidase (POx) from Aerococcus species were prepared by desolvation method, which were then characterized and covalently immobilized onto gold electrode (AuE) to construct an improved model of amperometric pyruvate biosensor. The POxNPs/Au electrode was analyzed morphologically by scanning electron microscopy (SEM). On the other hand, cyclic voltammetry studies (CV) and electrochemical impedance spectroscopy (EIS) helped in deciphering the electrochemical properties of the electrode at different stages of construction. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.006DOI Listing
April 2019
7 Reads

Protein engineering of Pseudomonas fluorescens peroxidase Dyp1B for oxidation of phenolic and polymeric lignin substrates.

Enzyme Microb Technol 2019 Apr 9;123:21-29. Epub 2019 Jan 9.

Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK. Electronic address:

Directed evolution was applied to dye-decolourizing peroxidase Dyp1B from Pseudomonas fluorescens Pf-5, in order to enhance the activity for oxidation of phenolic and lignin substrates. Saturation mutagenesis was used to generate focused libraries at 7 active site residues in the vicinity of the heme cofactor, and the libraries were screened for activity towards 2,6-dichlorophenol. Mutants N193 L and H169 L were found to show 7-8 fold enhanced k/K towards DCP, and replacements at Val205 and Ala209 also showed enhanced activity towards alkali Kraft lignin. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.002DOI Listing
April 2019
1 Read

A simplified method to remove fusion tags from a xylanase of Bacillus sp. HBP8 with HRV 3C protease.

Enzyme Microb Technol 2019 Apr 4;123:15-20. Epub 2019 Jan 4.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, People's Republic of China. Electronic address:

Human rhinovirus 3C protease (HRV 3C protease) is commonly used as a tool to remove fusion tags from recombinant proteins in gene engineering due to its distinguished specificity and high activity at low temperature. This paper is aimed to simplify the strategy of removing epitope tags from target proteins with HRV 3C protease. Fusion proteins composed of a xylanase from Bacillus sp. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.004DOI Listing
April 2019
2 Reads
2.322 Impact Factor

A mechanized urease activity assay.

Enzyme Microb Technol 2019 Apr 3;123:1-7. Epub 2019 Jan 3.

University of Warsaw, Faculty of Chemistry, L. Pasteura 1, 02-093, Warsaw, Poland.

Two fully mechanized flow analysis systems for urease activity assays have been developed, characterized and compared. Both of them are based on almost the same compact system of solenoid micropumps and microvalves controlled and actuated by highly effective, low-power and economic Arduino microcontroller. For photometric detection of ammonia formed in the course of enzymatic hydrolysis of urea, the Berthelot method and the Nessler reaction have been examined. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.001DOI Listing

Development of strategy for simultaneous enhanced production of alkaline xylanase-pectinase enzymes by a bacterial isolate in short submerged fermentation cycle.

Enzyme Microb Technol 2019 Mar 21;122:90-100. Epub 2018 Dec 21.

Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, India. Electronic address:

The aim of this study is to enhance the production of industrially valuable xylanase and pectinase enzymes in short duration, using agrowaste extracted substrates. Conventional cum statistical multifactor analysis approaches were used in order to evaluate the effect of crude extracted substrates, supplemented for the production of xylanase-pectinase enzymes. Incorporation of crude extracted xylan (1. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.008DOI Listing
March 2019
1 Read

Biosynthesis of (deoxy)guanosine-5'-triphosphate by GMP kinase and acetate kinase fixed on the surface of E. coli.

Enzyme Microb Technol 2019 Mar 27;122:82-89. Epub 2018 Dec 27.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. Electronic address:

(Deoxy)guanosine-5'-triphosphate (5'-(d)GTP), the precursor for synthesizing DNA or RNA in vivo, is an important raw material for various modern biotechnologies based on PCR. In this study, we investigated the application of whole-cell catalysts constructed by bacterial cell surface display in biosynthetic reactions of 5'-(d)GTP from (deoxy)guanosine-5'-monophosphate (5'-(d)GMP). By N-terminal or N- and C-terminal fusion of the ice nucleation protein, we successfully displayed the GMP kinase of Lactobacillus bulgaricus and the acetate kinase of E. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.011DOI Listing
March 2019
11 Reads

The effect of an oligosaccharide reducing-end xylanase, BhRex8A, on the synergistic degradation of xylan backbones by an optimised xylanolytic enzyme cocktail.

Enzyme Microb Technol 2019 Mar 19;122:74-81. Epub 2018 Dec 19.

Enzyme Science Programme (ESP), Department of Biochemistry and Microbiology, Rhodes University, Grahamstown 6140, Eastern Cape, South Africa. Electronic address:

Xylan, the most abundant hemicellulose in lignocellulosic biomass, requires a consortium of xylanolytic enzymes to achieve its complete de-polymerisation. As global interest in using xylan-containing lignocellulosic feedstocks for biofuel production increases, an accompanying knowledge on how to efficiently depolymerise these feedstocks into fermentable sugars is required. Since it has been observed that the same enzyme [i. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.010DOI Listing
March 2019
1 Read

Optimization and modelling of enzymatic polymerization of ε-caprolactone to polycaprolactone using Candida Antartica Lipase B with response surface methodology and artificial neural network.

Enzyme Microb Technol 2019 Mar 5;122:7-18. Epub 2018 Dec 5.

Department of Chemical and Environmental Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500, Semenyih, Selangor Darul Ehsan, Malaysia. Electronic address:

Recently enzymatic catalysts have replaced organic and organometallic catalysts in the synthesis of bio-resorbable polymers. Enzymatic polymerization is considered as an alternative to conventional polymerization as they are less toxic, environmental friendly and can operate under mild conditions. In this research, the enzymatic ring-opening polymerization (e-ROP) of e-caprolactone (e-CL) using Candida Antartica Lipase B (CALB) as catalyst to produce the Polycaprolactone. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.001DOI Listing
March 2019
9 Reads

Laccase activity measurement by FTIR spectral fingerprinting.

Enzyme Microb Technol 2019 Mar 18;122:64-73. Epub 2018 Dec 18.

Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs. Lyngby, 2800, Denmark. Electronic address:

Laccases (EC 1.10.3. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.009DOI Listing
March 2019
8 Reads

Functional expression of porcine interferon-α using a combinational strategy in Pichia pastoris GS115.

Enzyme Microb Technol 2019 Mar 10;122:55-63. Epub 2018 Dec 10.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Engineering Research Center for Bio-enzyme Catalysis, Hubei Key Laboratory of Industrial Biotechnology, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, College of Life Sciences, Hubei University, Wuhan, 430062, China. Electronic address:

Porcine interferon-α (pIFN-α) could be used as the vaccine adjuvant to enhance the antiviral ability of porcine in swine industry. In here, a combinational strategy integrating codon optimization, multiple gene insertion, strong AOX1 promoter, and efficient secretion signal sequence was developed to obtain high-level secreted pIFN-α in Pichia pastoris GS115. The codon optimized pIFN-α shared 76% sequence identity with the original pIFN-α, which was inserted into the P. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183050
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http://dx.doi.org/10.1016/j.enzmictec.2018.12.005DOI Listing
March 2019
15 Reads

Identification and characterization of a sterically robust phenylalanine ammonia-lyase among 481 natural isoforms through association of in silico and in vitro studies.

Enzyme Microb Technol 2019 Mar 12;122:36-54. Epub 2018 Dec 12.

Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran; Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address:

The enzyme phenylalanine ammonia lyase (PAL) is of special importance for the treatment of phenylketonuria patients. The aim of this study was to find a stable recombinant PAL with suitable kinetic properties among all natural PAL producing species using in silico and experimental approaches. To find such a stable PAL among 481 natural isoforms, 48,000 of 3-D models were predicted using the Modeller 9. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.006DOI Listing
March 2019
3 Reads

Horseradish peroxidase immobilized on the magnetic composite microspheres for high catalytic ability and operational stability.

Enzyme Microb Technol 2019 Mar 13;122:26-35. Epub 2018 Dec 13.

School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, China.

In the present study, a novel and efficient immobilization for horseradish peroxidase (HRP) had been developed by using 6-arm magnetic composite microsphere (FeO@PAA-6-arm-PEG-NH) containing 6-arm polyethylene glycol (6-arm-PEG-NH) and FeO. The morphology and chemical properties of FeO@PAA-6-arm-PEG-NH were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectra (FTIR), X-ray powder diffraction (XRD), vibrating sample magnetometer (VSM) and thermogravimetric analysis (TGA). The immobilization conditions and loading capacity of the carrier toward HRP were also investigated. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.007DOI Listing
March 2019
2 Reads

Fructosylation of phenolic compounds by levansucrase from Gluconacetobacter diazotrophicus.

Enzyme Microb Technol 2019 Mar 4;122:19-25. Epub 2018 Dec 4.

LISBP, Université de Toulouse, CNRS, INRA, INSA, LISBP-INSA Toulouse 135 Avenue de Rangueil, 31077, Toulouse, France. Electronic address:

Fructosylation can significantly improve the solubility, stability and bioactivity of phenolic compounds, increasing their health benefits. Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183053
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http://dx.doi.org/10.1016/j.enzmictec.2018.12.004DOI Listing
March 2019
12 Reads

The effects of NaCl on enzyme encapsulation by zeolitic imidazolate frameworks-8.

Enzyme Microb Technol 2019 Mar 3;122:1-6. Epub 2018 Dec 3.

Nanjing University of Science and Technology, Department of Bioengineering, Xiaolingwei 200, 210094, Nanjing, China.

Metal-organic framework (MOF) has generated a lot of research interests for it can be employed as immobilization material for enzyme. There are many small molecules in enzyme solution during the extraction process, such as NaCl. It is important to study the effects of the small molecules on MOFs. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.003DOI Listing
March 2019
3 Reads

Simultaneous synthesis of l-DOPA and oxidation of d-amino acid by specific coupling of a peroxidase to d-amino acid oxidase.

Enzyme Microb Technol 2019 Feb 29;121:8-16. Epub 2018 Oct 29.

Department of Biochemical Engineering, Beijing University of Chemical Technology, Beijing, China. Electronic address:

3,4 Dihydroxy phenyl l-alanine (L-DOPA) is the gold standard Parkinson's disease therapy. A heme-dependent peroxidase (HDP) catalyzes the ortho-hydroxylation of l-tyrosine to l-DOPA using HO as the co-substrate. d-amino acid oxidase (DAAO) catalyzes the oxidative deamination of d-amino acids (e. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183052
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http://dx.doi.org/10.1016/j.enzmictec.2018.10.013DOI Listing
February 2019
11 Reads
2.322 Impact Factor

An efficient thermostabilization strategy based on self-assembling amphipathic peptides for fusion tags.

Enzyme Microb Technol 2019 Feb 17;121:68-77. Epub 2018 Nov 17.

National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China; School of Biotechnology, Jiangnan University, Wuxi, 214122, China. Electronic address:

Self-assembling amphipathic peptides (SAPs) have been used as stabilization tags to improve enzyme stability but do not function uniformly well with all target enzymes. Here, the key factors involved in SAPs stabilization were identified as the SAP length and linker length and flexibility, using S1 (AEAEAKAK) as an originated SAP and polygalacturonate lyase (PGL) as model protein. Biochemical analysis demonstrated that SAPs could induce loose protein oligomerization via intermolecular hydrophobic interactions. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183050
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http://dx.doi.org/10.1016/j.enzmictec.2018.11.004DOI Listing
February 2019
9 Reads

Efficient immobilization of firefly luciferase in a metal organic framework: Fe-MIL-88(NH) as a mighty support for this purpose.

Enzyme Microb Technol 2019 Feb 29;121:59-67. Epub 2018 Oct 29.

Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address:

We investigated the mechanism of luciferase immobilization on a solid surface through therphtalaldehyde in order to understand the role of this linker on stability and activity of luciferase. Metal organic frameworks (MOFs) are capable supports for the stabilization of some proteins and biomolecules, therefore, in this context for the first time, we report a light emmiting enzyme immobillization on one of these supports and then outline chemical developments in this process. To this end, Fe-MIL-88(NH2) was used to immobilize native luciferase and therphtalaldehyde linker was attached to the framework as an activated group. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.10.011DOI Listing
February 2019
1 Read

Convenient preparation of sagittatoside B, a rare bioactive secondary flavonol glycoside, by recyclable and integrated biphase enzymatic hydrolysis.

Enzyme Microb Technol 2019 Feb 3;121:51-58. Epub 2018 Dec 3.

School of Pharmacy, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China; School of Traditional Chinese Pharmacy, China Pharmaceutical University, 639 Longmian Avenue, Nanjing, 211198, China. Electronic address:

Sagittatoside B, a rare secondary flavonol glycoside in Epimedii Folium, has much better in vivo bioactivities than its original glycoside epimedin B. Its preparation methods, such as acidic hydrolysis, are of low efficiency, and byproducts are generated. The objective of this study was to establish a novel catalysis system for convenient preparation of this compound based on recyclable and integrated biphase enzymatic hydrolysis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183041
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http://dx.doi.org/10.1016/j.enzmictec.2018.12.002DOI Listing
February 2019
33 Reads

Effect of glucose depletion during the synthesis of galactooligosaccharides using a trienzymatic system.

Enzyme Microb Technol 2019 Feb 23;121:45-50. Epub 2018 Oct 23.

Anhalt University of Applied Sciences, Department of Applied Biosciences and Process Engineering, Bernburger Str. 55, 06366, Köthen, Germany. Electronic address:

In this study the effect of glucose depletion using glucose oxidase and catalase, simultaneously to the synthesis of prebiotic galactooligosaccharides (GOS) by β-galactosidase was studied. Considering total GOS yield, a strong dependency on the source of β-galactosidase was found. Using an Aspergillus oryzae lactase, a small increase in GOS yield (from 50. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183037
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http://dx.doi.org/10.1016/j.enzmictec.2018.10.009DOI Listing
February 2019
4 Reads

Protein engineering to enhance keratinolytic protease activity and excretion in Escherichia coli and its scale-up fermentation for high extracellular yield.

Enzyme Microb Technol 2019 Feb 10;121:37-44. Epub 2018 Nov 10.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; School of Biotechnology, Jiangnan University, Wuxi 214122, China.

Escherichia coli is one kind of the simple and excellent biosystem to overexpress heterologous enzymes, such as keratinolytic protease, an excellent enzyme to hydrolyze keratin substrate for broad industrial application. However, protein expression in E. coli frequently faces some problems such as inactive and inclusion body formation. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.11.003DOI Listing
February 2019
1 Read