1,812 results match your criteria Enzyme and Microbial Technology [Journal]


Comparison of two models of surface display of xylose reductase in the Saccharomyces cerevisiae cell wall.

Enzyme Microb Technol 2019 Apr 6;123:8-14. Epub 2019 Jan 6.

Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10 000 Zagreb, Croatia. Electronic address:

In order to display xylose reductase at the surface of S. cerevisiae cells two different gene constructs have been prepared. In the first, xylose reductase gene GRE3 was fused with two parts of the CCW12 gene, the N-terminal one coding for the secretion signal sequence, and the C-terminal coding for the glycosylphosphatidylinositol anchoring signal. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.005DOI Listing

An improved enzyme nanoparticles based amperometric pyruvate biosensor for detection of pyruvate in serum.

Enzyme Microb Technol 2019 Apr 11;123:30-38. Epub 2019 Jan 11.

Department of Biochemistry, M.D. University, Rohtak, Haryana, India. Electronic address:

The nanoparticles of commercially available pyruvate oxidase (POx) from Aerococcus species were prepared by desolvation method, which were then characterized and covalently immobilized onto gold electrode (AuE) to construct an improved model of amperometric pyruvate biosensor. The POxNPs/Au electrode was analyzed morphologically by scanning electron microscopy (SEM). On the other hand, cyclic voltammetry studies (CV) and electrochemical impedance spectroscopy (EIS) helped in deciphering the electrochemical properties of the electrode at different stages of construction. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.006DOI Listing
April 2019
1 Read

Protein engineering of Pseudomonas fluorescens peroxidase Dyp1B for oxidation of phenolic and polymeric lignin substrates.

Enzyme Microb Technol 2019 Apr 9;123:21-29. Epub 2019 Jan 9.

Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK. Electronic address:

Directed evolution was applied to dye-decolourizing peroxidase Dyp1B from Pseudomonas fluorescens Pf-5, in order to enhance the activity for oxidation of phenolic and lignin substrates. Saturation mutagenesis was used to generate focused libraries at 7 active site residues in the vicinity of the heme cofactor, and the libraries were screened for activity towards 2,6-dichlorophenol. Mutants N193 L and H169 L were found to show 7-8 fold enhanced k/K towards DCP, and replacements at Val205 and Ala209 also showed enhanced activity towards alkali Kraft lignin. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.002DOI Listing

A simplified method to remove fusion tags from a xylanase of Bacillus sp. HBP8 with HRV 3C protease.

Enzyme Microb Technol 2019 Apr 4;123:15-20. Epub 2019 Jan 4.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, People's Republic of China. Electronic address:

Human rhinovirus 3C protease (HRV 3C protease) is commonly used as a tool to remove fusion tags from recombinant proteins in gene engineering due to its distinguished specificity and high activity at low temperature. This paper is aimed to simplify the strategy of removing epitope tags from target proteins with HRV 3C protease. Fusion proteins composed of a xylanase from Bacillus sp. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.004DOI Listing
April 2019
2.322 Impact Factor

A mechanized urease activity assay.

Enzyme Microb Technol 2019 Apr 3;123:1-7. Epub 2019 Jan 3.

University of Warsaw, Faculty of Chemistry, L. Pasteura 1, 02-093, Warsaw, Poland.

Two fully mechanized flow analysis systems for urease activity assays have been developed, characterized and compared. Both of them are based on almost the same compact system of solenoid micropumps and microvalves controlled and actuated by highly effective, low-power and economic Arduino microcontroller. For photometric detection of ammonia formed in the course of enzymatic hydrolysis of urea, the Berthelot method and the Nessler reaction have been examined. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.01.001DOI Listing

Development of strategy for simultaneous enhanced production of alkaline xylanase-pectinase enzymes by a bacterial isolate in short submerged fermentation cycle.

Enzyme Microb Technol 2019 Mar 21;122:90-100. Epub 2018 Dec 21.

Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, India. Electronic address:

The aim of this study is to enhance the production of industrially valuable xylanase and pectinase enzymes in short duration, using agrowaste extracted substrates. Conventional cum statistical multifactor analysis approaches were used in order to evaluate the effect of crude extracted substrates, supplemented for the production of xylanase-pectinase enzymes. Incorporation of crude extracted xylan (1. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.008DOI Listing
March 2019
1 Read

Biosynthesis of (deoxy)guanosine-5'-triphosphate by GMP kinase and acetate kinase fixed on the surface of E. coli.

Enzyme Microb Technol 2019 Mar 27;122:82-89. Epub 2018 Dec 27.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. Electronic address:

(Deoxy)guanosine-5'-triphosphate (5'-(d)GTP), the precursor for synthesizing DNA or RNA in vivo, is an important raw material for various modern biotechnologies based on PCR. In this study, we investigated the application of whole-cell catalysts constructed by bacterial cell surface display in biosynthetic reactions of 5'-(d)GTP from (deoxy)guanosine-5'-monophosphate (5'-(d)GMP). By N-terminal or N- and C-terminal fusion of the ice nucleation protein, we successfully displayed the GMP kinase of Lactobacillus bulgaricus and the acetate kinase of E. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.011DOI Listing
March 2019
4 Reads

The effect of an oligosaccharide reducing-end xylanase, BhRex8A, on the synergistic degradation of xylan backbones by an optimised xylanolytic enzyme cocktail.

Enzyme Microb Technol 2019 Mar 19;122:74-81. Epub 2018 Dec 19.

Enzyme Science Programme (ESP), Department of Biochemistry and Microbiology, Rhodes University, Grahamstown 6140, Eastern Cape, South Africa. Electronic address:

Xylan, the most abundant hemicellulose in lignocellulosic biomass, requires a consortium of xylanolytic enzymes to achieve its complete de-polymerisation. As global interest in using xylan-containing lignocellulosic feedstocks for biofuel production increases, an accompanying knowledge on how to efficiently depolymerise these feedstocks into fermentable sugars is required. Since it has been observed that the same enzyme [i. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.010DOI Listing
March 2019
1 Read

Optimization and modelling of enzymatic polymerization of ε-caprolactone to polycaprolactone using Candida Antartica Lipase B with response surface methodology and artificial neural network.

Enzyme Microb Technol 2019 Mar 5;122:7-18. Epub 2018 Dec 5.

Department of Chemical and Environmental Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500, Semenyih, Selangor Darul Ehsan, Malaysia. Electronic address:

Recently enzymatic catalysts have replaced organic and organometallic catalysts in the synthesis of bio-resorbable polymers. Enzymatic polymerization is considered as an alternative to conventional polymerization as they are less toxic, environmental friendly and can operate under mild conditions. In this research, the enzymatic ring-opening polymerization (e-ROP) of e-caprolactone (e-CL) using Candida Antartica Lipase B (CALB) as catalyst to produce the Polycaprolactone. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.001DOI Listing
March 2019
9 Reads

Laccase activity measurement by FTIR spectral fingerprinting.

Enzyme Microb Technol 2019 Mar 18;122:64-73. Epub 2018 Dec 18.

Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs. Lyngby, 2800, Denmark. Electronic address:

Laccases (EC 1.10.3. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.009DOI Listing
March 2019
8 Reads

Functional expression of porcine interferon-α using a combinational strategy in Pichia pastoris GS115.

Enzyme Microb Technol 2019 Mar 10;122:55-63. Epub 2018 Dec 10.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Engineering Research Center for Bio-enzyme Catalysis, Hubei Key Laboratory of Industrial Biotechnology, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, College of Life Sciences, Hubei University, Wuhan, 430062, China. Electronic address:

Porcine interferon-α (pIFN-α) could be used as the vaccine adjuvant to enhance the antiviral ability of porcine in swine industry. In here, a combinational strategy integrating codon optimization, multiple gene insertion, strong AOX1 promoter, and efficient secretion signal sequence was developed to obtain high-level secreted pIFN-α in Pichia pastoris GS115. The codon optimized pIFN-α shared 76% sequence identity with the original pIFN-α, which was inserted into the P. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183050
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http://dx.doi.org/10.1016/j.enzmictec.2018.12.005DOI Listing
March 2019
4 Reads

Identification and characterization of a sterically robust phenylalanine ammonia-lyase among 481 natural isoforms through association of in silico and in vitro studies.

Enzyme Microb Technol 2019 Mar 12;122:36-54. Epub 2018 Dec 12.

Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran; Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address:

The enzyme phenylalanine ammonia lyase (PAL) is of special importance for the treatment of phenylketonuria patients. The aim of this study was to find a stable recombinant PAL with suitable kinetic properties among all natural PAL producing species using in silico and experimental approaches. To find such a stable PAL among 481 natural isoforms, 48,000 of 3-D models were predicted using the Modeller 9. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.006DOI Listing
March 2019
2 Reads

Horseradish peroxidase immobilized on the magnetic composite microspheres for high catalytic ability and operational stability.

Enzyme Microb Technol 2019 Mar 13;122:26-35. Epub 2018 Dec 13.

School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, China.

In the present study, a novel and efficient immobilization for horseradish peroxidase (HRP) had been developed by using 6-arm magnetic composite microsphere (FeO@PAA-6-arm-PEG-NH) containing 6-arm polyethylene glycol (6-arm-PEG-NH) and FeO. The morphology and chemical properties of FeO@PAA-6-arm-PEG-NH were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectra (FTIR), X-ray powder diffraction (XRD), vibrating sample magnetometer (VSM) and thermogravimetric analysis (TGA). The immobilization conditions and loading capacity of the carrier toward HRP were also investigated. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.007DOI Listing
March 2019
2 Reads

Fructosylation of phenolic compounds by levansucrase from Gluconacetobacter diazotrophicus.

Enzyme Microb Technol 2019 Mar 4;122:19-25. Epub 2018 Dec 4.

LISBP, Université de Toulouse, CNRS, INRA, INSA, LISBP-INSA Toulouse 135 Avenue de Rangueil, 31077, Toulouse, France. Electronic address:

Fructosylation can significantly improve the solubility, stability and bioactivity of phenolic compounds, increasing their health benefits. Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183053
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http://dx.doi.org/10.1016/j.enzmictec.2018.12.004DOI Listing
March 2019
3 Reads

The effects of NaCl on enzyme encapsulation by zeolitic imidazolate frameworks-8.

Enzyme Microb Technol 2019 Mar 3;122:1-6. Epub 2018 Dec 3.

Nanjing University of Science and Technology, Department of Bioengineering, Xiaolingwei 200, 210094, Nanjing, China.

Metal-organic framework (MOF) has generated a lot of research interests for it can be employed as immobilization material for enzyme. There are many small molecules in enzyme solution during the extraction process, such as NaCl. It is important to study the effects of the small molecules on MOFs. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.12.003DOI Listing
March 2019
2 Reads

Simultaneous synthesis of l-DOPA and oxidation of d-amino acid by specific coupling of a peroxidase to d-amino acid oxidase.

Enzyme Microb Technol 2019 Feb 29;121:8-16. Epub 2018 Oct 29.

Department of Biochemical Engineering, Beijing University of Chemical Technology, Beijing, China. Electronic address:

3,4 Dihydroxy phenyl l-alanine (L-DOPA) is the gold standard Parkinson's disease therapy. A heme-dependent peroxidase (HDP) catalyzes the ortho-hydroxylation of l-tyrosine to l-DOPA using HO as the co-substrate. d-amino acid oxidase (DAAO) catalyzes the oxidative deamination of d-amino acids (e. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183052
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http://dx.doi.org/10.1016/j.enzmictec.2018.10.013DOI Listing
February 2019
4 Reads
2.322 Impact Factor

An efficient thermostabilization strategy based on self-assembling amphipathic peptides for fusion tags.

Enzyme Microb Technol 2019 Feb 17;121:68-77. Epub 2018 Nov 17.

National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China; School of Biotechnology, Jiangnan University, Wuxi, 214122, China. Electronic address:

Self-assembling amphipathic peptides (SAPs) have been used as stabilization tags to improve enzyme stability but do not function uniformly well with all target enzymes. Here, the key factors involved in SAPs stabilization were identified as the SAP length and linker length and flexibility, using S1 (AEAEAKAK) as an originated SAP and polygalacturonate lyase (PGL) as model protein. Biochemical analysis demonstrated that SAPs could induce loose protein oligomerization via intermolecular hydrophobic interactions. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183050
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http://dx.doi.org/10.1016/j.enzmictec.2018.11.004DOI Listing
February 2019
6 Reads

Efficient immobilization of firefly luciferase in a metal organic framework: Fe-MIL-88(NH) as a mighty support for this purpose.

Enzyme Microb Technol 2019 Feb 29;121:59-67. Epub 2018 Oct 29.

Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address:

We investigated the mechanism of luciferase immobilization on a solid surface through therphtalaldehyde in order to understand the role of this linker on stability and activity of luciferase. Metal organic frameworks (MOFs) are capable supports for the stabilization of some proteins and biomolecules, therefore, in this context for the first time, we report a light emmiting enzyme immobillization on one of these supports and then outline chemical developments in this process. To this end, Fe-MIL-88(NH2) was used to immobilize native luciferase and therphtalaldehyde linker was attached to the framework as an activated group. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.10.011DOI Listing
February 2019
1 Read

Convenient preparation of sagittatoside B, a rare bioactive secondary flavonol glycoside, by recyclable and integrated biphase enzymatic hydrolysis.

Enzyme Microb Technol 2019 Feb 3;121:51-58. Epub 2018 Dec 3.

School of Pharmacy, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China; School of Traditional Chinese Pharmacy, China Pharmaceutical University, 639 Longmian Avenue, Nanjing, 211198, China. Electronic address:

Sagittatoside B, a rare secondary flavonol glycoside in Epimedii Folium, has much better in vivo bioactivities than its original glycoside epimedin B. Its preparation methods, such as acidic hydrolysis, are of low efficiency, and byproducts are generated. The objective of this study was to establish a novel catalysis system for convenient preparation of this compound based on recyclable and integrated biphase enzymatic hydrolysis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183041
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http://dx.doi.org/10.1016/j.enzmictec.2018.12.002DOI Listing
February 2019
17 Reads

Effect of glucose depletion during the synthesis of galactooligosaccharides using a trienzymatic system.

Enzyme Microb Technol 2019 Feb 23;121:45-50. Epub 2018 Oct 23.

Anhalt University of Applied Sciences, Department of Applied Biosciences and Process Engineering, Bernburger Str. 55, 06366, Köthen, Germany. Electronic address:

In this study the effect of glucose depletion using glucose oxidase and catalase, simultaneously to the synthesis of prebiotic galactooligosaccharides (GOS) by β-galactosidase was studied. Considering total GOS yield, a strong dependency on the source of β-galactosidase was found. Using an Aspergillus oryzae lactase, a small increase in GOS yield (from 50. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183037
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http://dx.doi.org/10.1016/j.enzmictec.2018.10.009DOI Listing
February 2019
4 Reads

Protein engineering to enhance keratinolytic protease activity and excretion in Escherichia coli and its scale-up fermentation for high extracellular yield.

Enzyme Microb Technol 2019 Feb 10;121:37-44. Epub 2018 Nov 10.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; School of Biotechnology, Jiangnan University, Wuxi 214122, China.

Escherichia coli is one kind of the simple and excellent biosystem to overexpress heterologous enzymes, such as keratinolytic protease, an excellent enzyme to hydrolyze keratin substrate for broad industrial application. However, protein expression in E. coli frequently faces some problems such as inactive and inclusion body formation. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.11.003DOI Listing
February 2019
1 Read

Deciphering the inhibition effect of thymoquinone on xanthine oxidase activity using differential pulse voltammetry in combination with theoretical studies.

Enzyme Microb Technol 2019 Feb 2;121:29-36. Epub 2018 Nov 2.

Department of Chemistry, Faculty of Science, Yazd University, Yazd, 89195-741, Iran.

Xanthine oxidase (XO) catalyzes the oxidation of xanthine to uric acid. Over-production of uric acid is a risk factor for hyperuricemia and other diseases. Although allopurinol decreases uric acid levels, it causes severe adverse effects. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.11.002DOI Listing
February 2019
2 Reads

Modeling of an electrochemical nanobiosensor in COMSOL Multiphysics to determine phenol in the presence of horseradish peroxidase enzyme.

Enzyme Microb Technol 2019 Feb 3;121:23-28. Epub 2018 Nov 3.

Department of Chemistry, Yazd University, Yazd, 89195-741, Iran. Electronic address:

Horseradish peroxidase enzyme selectively oxidizes phenol to o-quinone that can be reduced electrochemically to catechol and generating a current response which is directly proportional to phenol concentration. In order to investigate the o-quinone enzymatic production and its electrochemical behavior, a 2-D model was developed for a nanochip biosensor in COMSOL Multiphysics. The oxidation rate of phenol to o-quinone was predicted by the developed model based on Michaelis-Menten equation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183042
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http://dx.doi.org/10.1016/j.enzmictec.2018.11.001DOI Listing
February 2019
6 Reads

Secretory overexpression of the endoglucanase by Saccharomyces cerevisiae via CRISPR-δ-integration and multiple promoter shuffling.

Enzyme Microb Technol 2019 Feb 31;121:17-22. Epub 2018 Oct 31.

Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, Japan.

Various recombinant proteins can be produced by the yeast Saccharomyces cerevisiae cell factories; therefore, efficient recombinant protein production techniques are desirable. In this study, to establish an efficient recombinant protein production technique in S. cerevisiae, the secretory production of recombinant protein endoglucanase II (TrEG) was tested. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183045
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http://dx.doi.org/10.1016/j.enzmictec.2018.10.014DOI Listing
February 2019
5 Reads

Cell entry of BmCPV can be promoted by tyrosine-protein kinase Src64B-like protein.

Enzyme Microb Technol 2019 Feb 29;121:1-7. Epub 2018 Oct 29.

School of Biology and Basic Medical Sciences, Soochow University, Suzhou, 215123, China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, 215123, China; Agricultural Biotechnology Research Institute, Agricultural biotechnology and Ecological Research Institute, Soochow University, Suzhou, 215123, China. Electronic address:

Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is a non-enveloped dsRNA virus, which specifically infect the midgut epithelium of B. mori. BmCPV enters permissive cells via clathrin-dependent endocytosis employing β1 integrin mediated internalization. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.10.012DOI Listing
February 2019
2 Reads
2.322 Impact Factor

Bioprocess optimization for the overproduction of catalytic domain of ubiquitin-like protease 1 (Ulp1) from S. cerevisiae in E. coli fed-batch culture.

Enzyme Microb Technol 2019 Jan 21;120:98-109. Epub 2018 Oct 21.

Department of Microbiology, University of Delhi South Campus, New Delhi, 110 021, India. Electronic address:

The exploitation of SUMO (small ubiquitin-related modifier) fusion technology at a large scale for the production of therapeutic proteins with an authentic N-terminus is majorly limited due to the higher cost of ScUlp1 protease. Therefore, the cost-effective production of Saccharomyces cerevisiae Ulp1 protease catalytic domain (402-621 aa) was targeted via its cloning under strong T7 promoter with and without histidine tag. The optimization of cultivation conditions at shake flask resulted in ScUlp1 expression of 195 mg/L in TB medium with a specific product yield of 98 mg/g DCW. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183045
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http://dx.doi.org/10.1016/j.enzmictec.2018.10.008DOI Listing
January 2019
10 Reads

A single point mutation in hmgA leads to melanin accumulation in Bacillus thuringiensis BMB181.

Enzyme Microb Technol 2019 Jan 19;120:91-97. Epub 2018 Oct 19.

Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071, China; Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300071, China. Electronic address:

Bacillus thuringiensis BMB181 (Bt BMB181), with high melanin production, is an ideal candidate for industrial scale production of light-stable insecticides. However, its melanogenic pathways remain unclear. In the present study, we demonstrated that Bt BMB181 failed to produce melanin after treatment with mesotrione, an inhibitor of 4-hydroxyphenylpyruvate dioxygenase in the homogentisic acid pathway of melanin synthesis. Read More

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https://www.researchgate.net/publication/24184839_Insecticid
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https://www.researchgate.net/publication/24245124_Inactivati
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https://linkinghub.elsevier.com/retrieve/pii/S01410229183047
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http://dx.doi.org/10.1016/j.enzmictec.2018.10.007DOI Listing
January 2019
10 Reads

High-efficiency enzymatic production of α-isoquercitrin glucosides by amylosucrase from Deinococcus geothermalis.

Enzyme Microb Technol 2019 Jan 19;120:84-90. Epub 2018 Oct 19.

Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 17104, Republic of Korea; Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 17104, Republic of Korea. Electronic address:

Isoquercitrin (IQ, quercetin-3-O-β-d-glucopyranoside) has diverse biological functions, such as anti-oxidant and anti-cancer activity, but its use is limited by poor solubility and bioavailability. Enzymatically modified IQ (EMIQ) is a mixture of transglycosylated IQs that have better solubility and bioavailability than do quercetin and IQ. Two different enzymes, cyclodextrin glucanotransferase (CGTase) and amylosucrase (ASase), have the transglycosylation activity to produce EMIQ. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183049
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http://dx.doi.org/10.1016/j.enzmictec.2018.10.006DOI Listing
January 2019
5 Reads

A novel low pH fermentation process for the production of acetate and propylene glycol from carbohydrate wastes.

Enzyme Microb Technol 2019 Jan 21;120:8-15. Epub 2018 Sep 21.

Department of Civil Engineering, Kansas State University, Fiedler Hall, 1701C Platt Street, Manhattan, KS, 66502, United States. Electronic address:

A novel low pH fermentation process was studied for the conversion of lactose using Lactobacillus plantarum and Lactobacillus buchneri under anoxic conditions in single co-culture, and two-stage sequential fermentations. This is aimed at producing acetate and propylene glycol (PG) as environmentally benign substitutes for currently used road and aircraft deicing chemicals. The results indicate that in the case of two-stage fermentation with immobilized L. Read More

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https://www.sciencedirect.com/science/article/pii/S014102291
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https://linkinghub.elsevier.com/retrieve/pii/S01410229183051
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http://dx.doi.org/10.1016/j.enzmictec.2018.09.006DOI Listing
January 2019
7 Reads

Bilirubin oxidase-based silica macrocellular robust catalyst for on line dyes degradation.

Enzyme Microb Technol 2019 Jan 11;120:77-83. Epub 2018 Oct 11.

CNRS, Univ. Bordeaux, CRPP, UMR5031, 115 Avenue Albert Schweitzer, F-33600, Pessac, France. Electronic address:

We present a new heterogeneous biocatalyst based on the grafting of Bilirubin Oxidase from Bacillus pumilus into macrocellular Si(HIPE) materials dedicated to water treatment. Due to the host intrinsic high porosity and monolithic character, on-line catalytic process is reached. We thus used this biocatalyst toward uni-axial flux decolorizations of Congo Red and Remazol Brilliant Blue (RBBR) at two different pH (4 and 8. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183034
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http://dx.doi.org/10.1016/j.enzmictec.2018.10.004DOI Listing
January 2019
8 Reads

Glucansucrase catalyzed synthesis and functional characterization of nordihydroguaiaretic acid glucosides.

Enzyme Microb Technol 2019 Jan 5;120:69-76. Epub 2018 Oct 5.

Faculty of Applied Natural Sciences, TH Köln-Campus Leverkusen, Chempark Leverkusen E39, Kaiser-Wilhelm-Allee, 51368, Leverkusen, Germany. Electronic address:

Nordihydroguaiaretic acid (NDGA) is the major lignan of the creosote bush Larrea tridentata known for its antioxidative and pharmacological properties. Here we present the identification of glucansucrases for NDGA glucosylation and the physicochemical and biological characterization of the glucosides. Extracellular glucansucrase of L. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183043
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http://dx.doi.org/10.1016/j.enzmictec.2018.10.002DOI Listing
January 2019
9 Reads

Functional analysis of active amino acid residues of the mercaptosuccinate dioxygenase of Variovorax paradoxus B4.

Enzyme Microb Technol 2019 Jan 21;120:61-68. Epub 2018 Sep 21.

Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität, D-48149 Münster, Germany; Environmental Science Department, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address:

Thiol dioxygenases are non-heme mononuclear-iron proteins and belong to the cupin superfamily. In 2014, mercaptosuccinate dioxygenase (Msdo) of Variovorax paradoxus B4 was identified as another bacterial cysteine dioxygenase (Cdo) homolog catalyzing the conversion of mercaptosuccinate (MS) into succinate and sulfite. To gain further insights into potentially important amino acid residues for enzyme activity, seven enzyme variants were generated and analyzed. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183031
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http://dx.doi.org/10.1016/j.enzmictec.2018.09.007DOI Listing
January 2019
10 Reads

Enzymatic synthesis of sitagliptin intermediate using a novel ω-transaminase.

Enzyme Microb Technol 2019 Jan 6;120:52-60. Epub 2018 Oct 6.

Department of Systems Biotechnology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. Electronic address:

Enantiopure β-amino acids are essential precursors of various pharmaceuticals, agrochemicals and other industrially important chemicals. In this study, we selected sixteen potential ω-Transaminases (ω-TAs) by BLAST and phylogenetic tree analysis. These ω-TAs were cloned, purified and tested for their reactivity for the synthesis of model β-amino acid (R)-3-amino-4-(2,4,5-triflurophenyl) butanoic acid [3-ATfBA], a key precursor for sitagliptin. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183045
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http://dx.doi.org/10.1016/j.enzmictec.2018.10.003DOI Listing
January 2019
6 Reads

Early biofouling detection using fluorescence-based extracellular enzyme activity.

Enzyme Microb Technol 2019 Jan 5;120:43-51. Epub 2018 Oct 5.

Water Desalination and Reuse Center (WDRC), Biological and Environmental Science & Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Membrane-based filtration technologies have seen rapid inclusion in a variety of industrial processes, especially production of drinking water by desalination. Biological fouling of membranes is a challenge that leads to increased costs from efficiency reductions, membrane damage and ultimately, membrane replacement over time. Such costs can be mitigated by monitoring and optimizing cleaning processes for better prognosis. Read More

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https://www.researchgate.net/publication/328110746_Early_bio
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https://linkinghub.elsevier.com/retrieve/pii/S01410229183018
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http://dx.doi.org/10.1016/j.enzmictec.2018.10.001DOI Listing
January 2019
8 Reads

A facile method to oriented immobilization of His-tagged BirA on Co-NTA agarose beads.

Enzyme Microb Technol 2019 Jan 18;120:36-42. Epub 2018 Sep 18.

School of Life Science and Biotechnology, Dalian University of Technology, No. 2 Linggong Road, Dalian, Liaoning, 116023, China. Electronic address:

A facile and economical method was established for the oriented immobilization of biotin ligase (BirA) on Co-NTA sepharose through HO oxidation of Co and His-tag. His-tag of the BirA were designed at both N-terminal (His-BirA) and C-terminal (BirA-His), respectively. Immobilization of the His-BirA was performed, realized to 92. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.09.004DOI Listing
January 2019
6 Reads

Functional characterization and comparative analysis of two heterologous endoglucanases from diverging subfamilies of glycosyl hydrolase family 45.

Enzyme Microb Technol 2019 Jan 27;120:23-35. Epub 2018 Sep 27.

Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena, SP, Brazil. Electronic address:

Lignocellulosic materials are abundant, renewable and are emerging as valuable substrates for many industrial applications such as the production of second-generation biofuels, green chemicals and pharmaceuticals. However, the recalcitrance and the complexity of cell wall polysaccharides require multiple enzymes for their complete conversion to oligo- and monosaccharides. The endoglucanases from GH45 family are a small and relatively poorly studied group of enzymes with potential industrial application. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.09.005DOI Listing
January 2019
8 Reads

Xylanase from Aspergillus tamarii shows different kinetic parameters and substrate specificity in the presence of ferulic acid.

Enzyme Microb Technol 2019 Jan 28;120:16-22. Epub 2018 Sep 28.

Enzymology Laboratory, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF, 70910-900, Brazil.

A 22 kDa xylanase (AtXyl1) from Aspergillus tamarii was purified by two chromatographic steps and presented preference for oat spelt (OSX), birchwood (BrX) and beechwood (BeX) xylans respectively, as substrates. AtXyl1 displays the highest activity at pH 5.5 and 55 °C and showed tolerance over a range of different phenolic compounds. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.09.009DOI Listing
January 2019
16 Reads

Synergetic effect of Ni and 5-acrylamidobenzoboroxole functional groups anchoring on magnetic nanoparticles for enhanced immobilization of horseradish peroxidase.

Enzyme Microb Technol 2019 Jan 15;120:136-143. Epub 2018 Jun 15.

School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, PR China.

In this work, a novel Ni&AABOB bifunctional core-shell magnetic composite nanoparticles (MNPs), FeO@p(AABOB-co-AIM-Ni), were synthesized for the immobilization of horseradish peroxidase (HRP). Two kinds of interaction are simultaneously incorporated to improve the stability and reusability of immobilized HRP: one is the coordination between Ni and amide group in HRP, and the other is the interaction of the wulff-type boronic acid group with cis-diol-containing HRP which belongs to a type of glycoproteins. Accordingly, the proposed approach achieved high enrichment capacity (174. Read More

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https://www.sciencedirect.com/journal/enzyme-and-microbial-t
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http://dx.doi.org/10.1016/j.enzmictec.2018.06.002DOI Listing
January 2019
8 Reads

Screening of novel feruloyl esterases from Talaromyces wortmannii for the development of efficient and sustainable syntheses of feruloyl derivatives.

Enzyme Microb Technol 2019 Jan 30;120:124-135. Epub 2018 Aug 30.

Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, SE-97187, Luleå, Sweden. Electronic address:

The feruloyl esterases Fae125, Fae7262 and Fae68 from Talaromyces wortmannii were screened in 10 different solvent: buffer systems in terms of residual hydrolytic activity and of the ability for the transesterification of vinyl ferulate with prenol or l-arabinose. Among the tested enzymes, the acetyl xylan-related Fae125 belonging to the phylogenetic subfamily 5 showed highest yield and selectivity for both products in alkane: buffer systems (n-hexane or n-octane). Response surface methodology, based on a 5-level and 6-factor central composite design, revealed that the substrate molar ratio and the water content were the most significant variables for the bioconversion yield and selectivity. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.08.007DOI Listing
January 2019
10 Reads

Evaluation of angiotensin converting enzyme inhibitors by SPR biosensor and theoretical studies.

Enzyme Microb Technol 2019 Jan 23;120:117-123. Epub 2018 Oct 23.

Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 14176-53955, Iran; Drug Design and Development Research Center, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, 14176-53955, Iran. Electronic address:

Surface plasmon resonance (SPR) biosensor has been utilized for monitoring analyte-ligand interactions in modern drug discovery processes. SPR biosensors measure the change in refractive indexes over the course of analyte molecules' binding to a specific immobilized ligand on sensor chip. This effort highlights a comprehensive SPR study besides enzymatic assay for discovery of new Angiotensin Converting Enzyme (ACE) inhibitors via screening of medicinal plants. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.10.010DOI Listing
January 2019
12 Reads

The role of enzyme adsorption in the enzymatic degradation of an aliphatic polyester.

Enzyme Microb Technol 2019 Jan 21;120:110-116. Epub 2018 Oct 21.

Laboratory of Plastics and Rubber Technology, Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, H-1521 Budapest, P.O. Box 91, Hungary; Polymer Physics Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, H-1519 Budapest, P.O. Box 286, Hungary. Electronic address:

The enzyme catalyzed degradation of poly(3-hydroxybutyrate) (PHB) is a two-step process consisting of the adsorption of the enzyme on the surface of a PHB substrate and the cleavage of ester bonds. A deactivated enzyme was prepared by point mutagenesis to separate the two steps from each other. Measurements carried out with active and inactive enzymes on PHB particles proved that mutagenesis was successful and the modified enzyme did not catalyze degradation. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.10.005DOI Listing
January 2019
2 Reads

Structural characterization and biological evaluation of Staphylosan (dimannooleate), a new glycolipid surfactant produced by a marine Staphylococcus saprophyticus SBPS-15.

Enzyme Microb Technol 2019 Jan 21;120:1-7. Epub 2018 Sep 21.

Faculty of Marine Sciences, Annamalai University, Porto Novo, Tamil Nadu 608502, India.

Marine microbes have gained significant attention as potential biofactories for broad spectrum bioactive compounds. In the recent years, bioactive biosurfactants have warranted renewed interest from both environmental and clinical sectors as anti-biofilm agents due to their excellent properties of dispersing microbial biofilms. The present study explores a new glycolipid biosurfactant produced by a marine Staphylococcus saprophyticus exhibiting interesting biological activities. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.09.008DOI Listing
January 2019
9 Reads

Directed evolution of a penicillin V acylase from Bacillus sphaericus to improve its catalytic efficiency for 6-APA production.

Enzyme Microb Technol 2018 Dec 24;119:65-70. Epub 2018 Aug 24.

Hunan Agricultural University, Changsha 410128, China. Electronic address:

Penicillin acylase is commonly used to produce the medical intermediates of 6-Aminopenicillanic acid (6-APA) and 7-Aminodesacetoxycephalosporanic acid (7-ADCA) in industrial process. Nowadays, Penicillin G acylase (PGA) has been widely applied for making pharmaceutical intermediates, while penicillin V acylase (PVA) has been less used for that due to its low activity and poor conversion. In this study, a PVA from Bacillus sphaericus (BspPVA) was employed for directed evolution study with hoping to increase its catalytic efficiency. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.08.006DOI Listing
December 2018
3 Reads

Polymerization of dopamine catalyzed by laccase: Comparison of enzymatic and conventional methods.

Enzyme Microb Technol 2018 Dec 10;119:58-64. Epub 2018 Sep 10.

Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, 1800 Lihu Ave., Wuxi, 214122, Jiangsu, PR China.

Polydopamine (PDA) films are generally produced by spontaneous oxidation of dopamine in basic medium, which is a highly convenient method for surface modification. This method is, however, not suitable for alkaline sensitive materials, which have poor stability in alkali medium and need over-long reaction time. Herein, we compared two dopamine polymerization methods: conventional method (induced by O at pH 8. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01410229183028
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http://dx.doi.org/10.1016/j.enzmictec.2018.09.003DOI Listing
December 2018
3 Reads

Altering the sensitivity of Escherichia coli pyruvate dehydrogenase complex to NADH inhibition by structure-guided design.

Enzyme Microb Technol 2018 Dec 5;119:52-57. Epub 2018 Sep 5.

Binzhou Medical University, Yantai, China. Electronic address:

A sufficient supply of reducing equivalents is essential for obtaining the maximum yield of target products in anaerobic fermentation. The pyruvate dehydrogenase (PDH) complex controls the critical step in pyruvate conversion to acetyl-CoA and NADH. However, in anaerobic Escherichia coli, PDH residing in the dihydrolipoamide dehydrogenase (LPD) component is normally inactive due to inhibition by NADH. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.09.002DOI Listing
December 2018
1 Read

Production of (Z)-11-(heptanoyloxy)undec-9-enoic acid from ricinoleic acid by utilizing crude glycerol as sole carbon source in engineered Escherichia coli expressing BVMO-ADH-FadL.

Enzyme Microb Technol 2018 Dec 5;119:45-51. Epub 2018 Sep 5.

Department of Environmental Engineering, College of Engineering, Ajou University, Suwon, Gyeonggi-do, South Korea. Electronic address:

Production of (Z)-11-(heptanoyloxy)undec-9-enoic acid from recinoleic acid was achieved by whole-cell biotransformation by Escherichia coli, utilizing crude glycerol as the sole carbon source. Whole-cell biotransformation resulted in ∼93% conversion of the substrate ricinoleic acid to (Z)-11-(heptanoyloxy)undec-9-enoic acid. We replaced the inducer-dependent promoter system (T7 and Rhm promotors) with a constitutive promoter system. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.09.001DOI Listing
December 2018
2 Reads

A sensitive and simple method for detecting Cu in plasma using fluorescent Bacillus amyloliquefaciens containing intracellularly biosynthesized CdSe quantum dots.

Enzyme Microb Technol 2018 Dec 1;119:37-44. Epub 2018 Sep 1.

Department of Analytical Chemistry, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, 24 Tongjia Lane, Gulou District, Nanjing 210009, China. Electronic address:

Bacillus amyloliquefaciens containing intracellularly biosynthesized cadmium selenide (CdSe) quantum dots (QDs) was used as a fluorescent bioprobe. Several parameters in the QD biosynthesis process were systematically optimized. The optimized protocol for producing high-quality CdSe QDs in B. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.08.009DOI Listing
December 2018
3 Reads

Cloning and protein expression of the sn-1(3) regioselective lipase from Cordyceps militaris.

Enzyme Microb Technol 2018 Dec 25;119:30-36. Epub 2018 Aug 25.

Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea; Center for Food and Bioconvergence, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea. Electronic address:

In this study, the gene of a novel lipase with sn-1(3) regioselectivity (i.e., sn-1 or sn-3 specific) from Cordyceps militaris was successfully expressed by a heterologous expression system. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.08.008DOI Listing
December 2018
2 Reads

Kinetic analysis of cellulose synthase of Gluconacetobacter hansenii in whole cells and in purified form.

Enzyme Microb Technol 2018 Dec 23;119:24-29. Epub 2018 Aug 23.

Department of Biochemistry and Molecular Biology, 305 South Frear, University Park, The Pennsylvania State University, PA, 16802, USA. Electronic address:

The Gram-negative bacterium, Gluconacetobacter hansenii, has been long studied and is a model for cellulose synthesis. It produces cellulose, using the enzyme AcsA-AcsB, of exceptionally high crystallinity in comparison to the cellulose of higher plants. We determined the rate of cellulose synthesis in whole cells measured as moles of glucose incorporated into cellulose per second per mole of enzyme. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.08.005DOI Listing
December 2018
2 Reads

Graphene enhanced transformation of lignin in laccase-ABTS system by accelerating electron transfer.

Enzyme Microb Technol 2018 Dec 18;119:17-23. Epub 2018 Aug 18.

Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, China.

The degradation of lignin has attracted much attention since it represents approximately 30% of all non-fossil carbon sources and constitutes a sustainable bio-resource for fuels and aromatic derivatives. Here we investigated the degradation of lignin by laccase-catalysed reactions using 2,2'-Azino-bis(3-ethybenzothiazoline-6-sulfonic acid) (ABTS) as a mediator coupled with the carbon material graphene. Results indicated that there was a significant, two-fold, increase in the catalytic activity of lignin degradation in laccase-ABTS systems in the presence of graphene. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.08.004DOI Listing
December 2018
10 Reads
2.320 Impact Factor