3,284,647 results match your criteria Enzyme and Microbial Technology [Journal]


Novel endotypes in heart failure: effects on guideline-directed medical therapy.

Eur Heart J 2018 Dec 13. Epub 2018 Dec 13.

Department of Cardiology, University of Groningen, Hanzeplein 1, Groningen, the Netherlands.

Aims: We sought to determine subtypes of patients with heart failure (HF) with a distinct clinical profile and treatment response, using a wide range of biomarkers from various pathophysiological domains.

Methods And Results: We performed unsupervised cluster analysis using 92 established cardiovascular biomarkers to identify mutually exclusive subgroups (endotypes) of 1802 patients with HF and reduced ejection fraction (HFrEF) from the BIOSTAT-CHF project. We validated our findings in an independent cohort of 813 patients. Read More

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December 2018

Advances and opportunities for the design of self-sufficient and spatially organized cell-free biocatalytic systems.

Curr Opin Chem Biol 2018 Dec 11;49:97-104. Epub 2018 Dec 11.

Heterogeneous biocatalysis laboratory, Instituto de Síntesis Química y Catálisis Homogénea (ISQCH-CSIC), University of Zaragoza, C/ Pedro Cerbuna 12, 50009, Zaragoza, Spain; ARAID, Aragon I+D foundation, Zaragoza, Spain. Electronic address:

During the past decades, biocatalysis has made important contributions to chemical manufacturing by using both whole-cell and cell-free biotransformation reactions. More recently, multi-enzyme systems that can run step-wise reactions in one-pot with high selectivity are increasingly being developed. The use of multiple isolated enzymes to perform a series of reactions offers operational and process advantages over the use of living or resting cells, but such cell free processes need to be optimized to meet industrial productivity and titer requirements. Read More

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December 2018
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Enhancement of excess sludge hydrolysis and decomposition with different lysozyme dosage.

J Hazard Mater 2018 Dec 3;366:395-401. Epub 2018 Dec 3.

School of Environment, Harbin Institute of Technology, Harbin 150090, China.

The performance of the lysozyme catalysis on excess sludge (ES) hydrolysis and decomposition was investigated in this study. For this purpose, the release of soluble organic matters from sludge flocs, extracellular polymeric substances (EPS) changes in composition and distribution and the quantity variations of microorganisms were monitored. Results indicated that lysozyme boosted the ES hydrolysis significantly with approximately 236. Read More

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December 2018

Investigating the physiological mechanisms underlying Salicornia ramosissima response to atmospheric CO enrichment under coexistence of prolonged soil flooding and saline excess.

Plant Physiol Biochem 2018 Dec 6;135:149-159. Epub 2018 Dec 6.

Departamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, 1095, 41080, Sevilla, Spain.

A 45-days long climatic chamber experiment was design to evaluate the effect of 400 and 700 ppm atmospheric CO treatments with and without soil water logging in combination with 171 and 510 mM NaCl in the halophyte Salicornia ramosissima. In order to ascertain the possible synergetic impact of these factors associate to climatic change in this plant species physiological and growth responses. Our results indicated that elevated atmospheric CO concentration improved plant physiological performance under suboptimal root-flooding and saline conditions plants. Read More

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December 2018

A tick beat in the electrocardiogram: Persistent third degree block as only manifestation of Lyme disease.

J Electrocardiol 2018 Dec 6;52:109-111. Epub 2018 Dec 6.

Internal Medicine Department, Hospital Universitario, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.

Lyme disease is the most common tick-borne illness in North America. A 23-year-old female presented to our emergency department with a chief complaint of sudden dyspnea and chest pain. An electrocardiogram revealed a third degree heart block. Read More

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December 2018

Biotinidase deficiency should be considered in individuals thought to have multiple sclerosis and related disorders.

Authors:
Barry Wolf

Mult Scler Relat Disord 2018 Nov 28;28:26-30. Epub 2018 Nov 28.

Division of Genetics, Birth Defects and Metabolism, Department of Pediatrics, Ann and Robert H. Lurie Children's Hospital of Chicago and Northwestern Feinberg School of Medicine Chicago, IL 60611, USA. Electronic address:

Background: Multiple sclerosis is a disorder of the central and peripheral nervous system of young and old adults that is characterized by muscle, coordination and vision abnormalities. Multiple sclerosis is likely due to numerous causes.

Methods: Recently, adolescents and adults with ophthalmological and or neurological findings have been diagnosed with biotinidase deficiency. Read More

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November 2018
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Highly Efficient Production of Chiral Amines in Batch and Continuous Flow by Immobilized ω-Transaminases on Controlled Porosity Glass Metal-Ion Affinity Carrier.

J Biotechnol 2018 Dec 11. Epub 2018 Dec 11.

Van' t Hoff Institute for Molecular Sciences, HIMS-Biocat & HetCat, University of Amsterdam, Science Park 904, 1098 XH, The Netherlands. Electronic address:

In this study, two stereocomplementary ω-transaminases from Arthrobacter sp. (AsR-ωTA) and Chromobacterium violaceum (Cv-ωTA) were immobilized via iron cation affinity binding onto polymer-coated controlled porosity glass beads (EziG). The immobilization procedure was studied with different types of carrier materials and immobilization buffers of varying compositions, concentrations, pHs and cofactor (PLP) concentrations. Read More

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December 2018

Research Update and Opportunity of Non-hormonal Male Contraception: Histone Demethylase KDM5B-Based Targeting.

Pharmacol Res 2018 Dec 11. Epub 2018 Dec 11.

Department of Animal Science & Technology and BET Research Institute, Chung-Ang University, Anseong, Gyeonggi-do, 17546, Republic of Korea. Electronic address:

With the continued increase in global human population, diverse contraception approaches have become increasingly essential, including non-hormonal male contraception. Non-hormonal approaches to contraception are very convenient; however, such options are limited because data regarding the identification and characterization of tissue/cell-specific targets and appropriate small molecule candidate contraceptives are lacking. Based on in-silico studies of genomics, transcriptomics, and proteomics, performed by mining datasets in PubMed, we first reviewed testis-, epididymis-, and germline cell-specific genes/proteins, with the aim of presenting evidence that many of these could become 'druggable' targets for the development of non-hormonal male contraceptives in the future. Read More

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December 2018

Multivariate analysis and modeling of soil quality indicators in long-term management systems.

Sci Total Environ 2018 Dec 4;657:457-465. Epub 2018 Dec 4.

Department of Plant Production, São Paulo State University-UNESP, 14884-900 Jaboticabal, SP, Brazil. Electronic address:

Soil management systems, as well as the long-term application of nitrogen fertilization, might promote changes in soil quality (SQ). The knowledge of how agronomic practices influence SQ is the main factor in the development of most sustainable management systems. Thus, the aim of this study was to evaluate the influence of long-term management systems on SQ through the analysis of 10 soil quality indicators (SQIs), to select the most sensitive SQIs through principal components analysis (PCA) and to propose a mathematical model that could estimate the activities of enzymes based on SQI values with simple and low-cost procedures in relation to enzyme measurement. Read More

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December 2018

Uridine diphosphate release mechanism in O-N-acetylglucosamine (O-GlcNAc) transferase catalysis.

Biochim Biophys Acta Gen Subj 2018 Dec 11. Epub 2018 Dec 11.

The Key Laboratory of Natural Medicine and Immuno-Engineering, Henan University, Kaifeng 475004, People's Republic of China. Electronic address:

O-linked N-acetylglucosamine transferase (OGT) is an essential enzyme that catalyzes the covalent bonding of N-acetylglucosamine (GlcNAc) to the hydroxyl group of a serine or threonine in the target protein. It plays an important role in many important cellular physiological catalytic reactions. Here, we determine the binding mode and the binding free energy of the OGT product (uridine diphosphate, UDP) as well as the hydrogen-bond-dependent release mechanism using extensive molecular dynamic simulations. Read More

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December 2018

Dimethyl fumarate ameliorates acetaminophen-induced hepatic injury in mice dependent of Nrf-2/HO-1 pathway.

Life Sci 2018 Dec 11. Epub 2018 Dec 11.

Department of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt. Electronic address:

Drug-induced liver toxicity is the most frequent cause of acute liver failure worldwide. Hepatotoxicity caused by acetaminophen (ACT) overdose is mediated by its metabolic product promoting oxidative stress and activation of inflammatory mediators. Nuclear factor erythroid-related factor-2 (Nrf-2) induces the release of cytoprotective enzymes in response to electrophilic or oxidative stress and is considered a promising therapeutic target. Read More

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December 2018

The IRS/PI3K/Akt signaling pathway mediates olanzapine-induced hepatic insulin resistance in male rats.

Life Sci 2018 Dec 11. Epub 2018 Dec 11.

Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Endocrinology and Metabolism Group, Sydney Institute of Health Sciences, Sydney Institute of Traditional Chinese Medicine, Sydney, NSW 2000, Australia. Electronic address:

Aims: Chronic treatment with antipsychotics, especially most of atypical ones, leads to development of metabolic abnormalities. Olanzapine is an atypical antipsychotic widely used in the treatment of schizophrenia and bipolar disorder. The mechanisms underlying olanzapine-induced metabolic adverse effects in the liver, however, remain unclear. Read More

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December 2018

USP14-mediated IκBα degradation exacerbates NF-κB activation and IL-1β-stimulated chondrocyte dedifferentiation.

Life Sci 2018 Dec 11. Epub 2018 Dec 11.

Rehabilitation Department, the Third Hospital of Xingtai City, Xingtai City, Hebei 054000, China.

Osteoarthritis (OA) is an inflammatory joint disease. USP14, a deubiquitinating enzyme critical for ubiquitin-mediated proteasomal protein degradation, is implicated in inflammation regulation. However, its role and mechanism in OA are poorly understood. Read More

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December 2018

Role of the androgen receptor gene CAG repeat polymorphism on the sequence of pubertal events and adiposity in girls with high dehydroepiandrosterone sulfate.

J Pediatr Adolesc Gynecol 2018 Dec 11. Epub 2018 Dec 11.

Institute of Maternal and Child Research, School of Medicine, University of Chile, Santiago, Chile. Electronic address:

Study Objective: The Androgen Receptor (AR) harbors a variable repeat of a variable number of glutamine residues [(CAG)n] which seems to affect inversely AR transcriptional activity. We assessed whether (CAG)n affect the sequence of the androgen-sensitive pubertal events and body composition in prepubertal girls.

Design: and participants: Nested case-control study within the Growth and Obesity Cohort Study of 1196 low-middle income children (≈50% girls) from Santiago, Chile. Read More

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December 2018

Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua).

Int J Biol Macromol 2018 Dec 11. Epub 2018 Dec 11.

Faculty of Food Science and Nutrition, School of Health Sciences, University of Iceland. University of Iceland, Sæmundargata 2, 101 Reykjavik, Iceland. Electronic address:

Atlantic cod trypsin ZT is biochemically characterized for the first time in this report in comparison to a group I trypsin (cod trypsin I). To our knowledge, trypsin ZT is the first thoroughly characterized group III trypsin. A more detailed understanding of trypsin ZT biochemistry may give insight into its physiological role as well as its potential use within the biotechnology sector. Read More

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December 2018

Deletion the C-terminal peptides of Vibrio harveyi phospholipase D significantly improved its enzymatic properties.

Int J Biol Macromol 2018 Dec 11. Epub 2018 Dec 11.

School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address:

A novel phospholipase D that originate from marine Vibrio harveyi (VhPLD) was recombinant expressed and biochemically characterized. Moreover, effects of C-terminal peptides on catalytic and interfacial binding properties of VhPLD were investigated by constructing two truncated mutants (VhPLD-Δ(472-483) and VhPLD-Δ(437-483)). Optimal reaction temperature and pH value for wild-type VhPLD (VhPLD-WT) was 45 °C and pH 8. Read More

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December 2018

Predicting drug-target interactions using Lasso with random forest based on evolutionary information and chemical structure.

Genomics 2018 Dec 11. Epub 2018 Dec 11.

College of Mathematics and Physics, Qingdao University of Science and Technology, Qingdao 266061, China; Artificial Intelligence and Biomedical Big Data Research Center, Qingdao University of Science and Technology, Qingdao 266061, China; School of Life Sciences, University of Science and Technology of China, Hefei 230027, China. Electronic address:

The identification of drug-target interactions has great significance for pharmaceutical scientific research. Since traditional experimental methods identifying drug-target interactions is costly and time-consuming, the use of machine learning methods to predict potential drug-target interactions has attracted widespread attention. This paper presents a novel drug-target interactions prediction method called LRF-DTIs. Read More

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December 2018

A structural and functional perspective on the enzymes of Mycobacterium tuberculosis involved in the L-rhamnose biosynthesis pathway.

Prog Biophys Mol Biol 2018 Dec 11. Epub 2018 Dec 11.

School of Chemistry, University of Hyderabad, Hyderabad, Telangana, 500046, India. Electronic address:

Tuberculosis is one of the leading causes of death from bacterial infections. The multi-drugresistant strain has warranted the development of new drug molecules which can inhibit the growth of Mycobacterium tuberculosis. Most of the known drugs inhibit the enzymes in the cell wall biosynthesis. Read More

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December 2018

A novel catalase mimicking nanocomposite of Mn(II)-poly-L-histidine-carboxylated multi walled carbon nanotubes and the application to hydrogen peroxide sensing.

Anal Biochem 2018 Dec 11. Epub 2018 Dec 11.

The Key Laboratory of Bioactive Materials Ministry of Education, College of Life Science, Nankai University, Weijin Road No.94, Tianjin, 300071, China. Electronic address:

In this work, a novel enzyme-mimicking nanocomposite of Mn(II)-poly-L-histidine (PLH) functionalized carboxylated multi walled carbon nanotubes (CMWCNTs) was designed and synthesized. Based on the catalase-like activity of the nanocomposite, a non-enzymatic hydrogen peroxide (H2O2) biosensor was then established and explored for H2O2 electrochemical detection. The nanocomposite was characterized by Fourier transform infrared spectra, Raman spectroscopy, and transmission electron microscopy. Read More

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December 2018
2.219 Impact Factor

The cytochrome b lysine 329 residue is critical for ubihydroquinone oxidation and proton release at the Q site of bacterial cytochrome bc.

Biochim Biophys Acta Bioenerg 2018 Dec 11. Epub 2018 Dec 11.

Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address:

The ubihydroquinone:cytochrome (cyt) c oxidoreductase (or cyt bc) is an important enzyme for photosynthesis and respiration. In bacteria like Rhodobacter capsulatus, this membrane complex has three subunits, the iron‑sulfur protein (ISP) with its FeS cluster, cyt c and cyt b, forming two catalytic domains, the Q (hydroquinone (QH) oxidation) and Q (quinone (Q) reduction) sites. At the Q site, the electron transfer pathways originating from QH oxidation are known, but their associated proton release routes are less well defined. Read More

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December 2018

Structural and functional insight into serine hydroxymethyltransferase from Helicobacter pylori.

PLoS One 2018 14;13(12):e0208850. Epub 2018 Dec 14.

Laboratory of Optics and Biosciences, Ecole polytechnique, CNRS, INSERM, Université Paris Saclay, Palaiseau, France.

Serine hydroxymethyltransferase (SHMT), encoded by the glyA gene, is a ubiquitous pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes the formation of glycine from serine. The thereby generated 5,10-methylene tetrahydrofolate (MTHF) is a major source of cellular one-carbon units and a key intermediate in thymidylate biosynthesis. While in virtually all eukaryotic and many bacterial systems thymidylate synthase ThyA, SHMT and dihydrofolate reductase (DHFR) are part of the thymidylate/folate cycle, the situation is different in organisms using flavin-dependent thymidylate synthase ThyX. Read More

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December 2018

Influence of pathway topology and functional class on the molecular evolution of human metabolic genes.

PLoS One 2018 14;13(12):e0208782. Epub 2018 Dec 14.

Institute of Systems Biology, Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain.

Metabolic networks comprise thousands of enzymatic reactions functioning in a controlled manner and have been shaped by natural selection. Thanks to the genome data, the footprints of adaptive (positive) selection are detectable, and the strength of purifying selection can be measured. This has made possible to know where, in the metabolic network, adaptive selection has acted and where purifying selection is more or less strong and efficient. Read More

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December 2018

Reaction Mechanism of a Nonheme Iron Enzyme Catalyzed Oxidative Cyclization via C-C Bond Formation.

Org Lett 2018 Dec 14. Epub 2018 Dec 14.

Department of Chemistry , National Taiwan Normal University , Taipei 11677 , Taiwan.

A complementary study including design of mechanistic probes, biochemical assays, model analysis, and liquid chromatography coupled mass spectrometry was conducted to establish the reaction mechanism for a nonheme iron enzyme catalyzed (-)-podophyllotoxin formation. Our results indicate that the originally proposed hydroxylated intermediate is unlikely to be involved in this reaction. Instead, the formation of benzylic radical/carbocation intermediate can be utilized to trigger the C-C bond formation to construct the C-ring of (-)-podophyllotoxin. Read More

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December 2018

Towards multi-scale modeling of proteins and bio-agglomerates: An orientation-sensitive diffusion model for the integration of Molecular Dynamics and the Discrete Element Method.

J Chem Inf Model 2018 Dec 14. Epub 2018 Dec 14.

Most processes involved in biological and biotechnological systems spread over many scales in space and time. For example, the interaction of multiple enzymes in heterogenous enzymatic agglomerates or clusters, necessary for efficient enzymatic conversion, is of high interest for research and enzyme engineering. In order to understand and predict their overall behavior and performance, it is important to describe these scales as completely as possible, known as multi-scale modeling. Read More

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December 2018

A Special Risk Group for Hepatitis E Infection: The First Record of North Cyprus.

Pol J Microbiol 2018 ;67(4):525-528

Department of Biophysics, Faculty of Medicine, Near East University , Nicosia , Cyprus.

Hepatitis E virus (HEV) is transmitted by a fecal oral route from animals to humans following exposure to the body fluids of infected animals. We investigated the seroprevalence of anti-hepatitis E (anti-HEV) antibodies by monitoring IgG and IgM virus antibodies amongst employees in the animal industry in North Cyprus through a cross-sectional study. Samples were taken from individuals without occupational exposure to animals and from those who worked with animals (doing animal husbandry, veterinary work or butchery). Read More

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January 2018

[Effect of butylphthalide on oxidative stress and cognitive function in old obstructive sleep apnea hypopnea syndrome patients].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2018 Sep;32(18):1422-1425

Department of Respiration of North China University of Science and Technology Affiliated Hospital, 063000, China.

To explore the effect of butylphthalide on oxidative stress and cognitive function in old obstructive sleep apnea hypopnea syndrome (OSAHS)patients.A total of 90 old OSAHS patients with cognitive impairment were choosed with Polysomnography(PSG). By random number table method OSAHS patients were randomly divided into non-invasive ventilator group (=30), butylphthalide with non-invasive ventilator group(=30) and treatment group of butylphthalide(=30). Read More

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September 2018

[The study of patterns of development of resistance of Staphylococcus aureus to triclosan].

Klin Lab Diagn 2018 ;63(1):60-64

The Federal budget institution of science "The state scientifc center of applied microbiology and biotechnology" of the Rospotrebnadzor, 142279, Obolensk, Moscow oblast, Russia.

The article presents the results of studying molecular genetic mechanisms of development of resistance to antiseptic Triclosan in strain Staphylococcus aureus АТСС25923. The modifcations of strain S. aureus АТСС25923 (Tr1, Tr2, Tr1С и Tr2С) are obtained resistant to 64 mg/l of Triclosan and stably preserving the given characteristic under cultivation in absence of selective pressure. Read More

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January 2018

[The clinical laboratory characteristic of primary acute cytomegalovirus infection in non-immunocompromised adult patients].

Klin Lab Diagn 2018 ;63(1):51-54

The state budget institution of health care "The clinical center of prevention and struggle with AIDS" of the Minzdrav of the Krasnodar kraii, 350015, Krasnodar, Russia.

The purpose of study is the clinical laboratory characteristic of acquired acute cytomegalovirus infection in nonimmunocompromised adult patients. The sampling included 75 patients with acquired acute cytomegalovirus infection admitted to the specialized clinical infection hospital of the Minzdrav of the Krasnodar kraii. The verifcation of diagnosis was implemented using serological tests and polymerase chain reaction. Read More

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January 2018

[The synchronous detection of serological markers of main herpes viral human infections].

Klin Lab Diagn 2018 ;63(1):35-40

The closed corporation "ECOlab", Elektrogorsk, Moscow oblast, Russia.

The new original national set of reagents "Line-Blot human herpesvirus-profle" was developed to detect antibodies (IgG - set 1 and IgM - set 2) to main agents of herpesvirus infections (HSV-1, HSV-2, VZV, EBV, CMV) in the format of linear immunoblotting. The preliminary clinical trials were implemented using 319 samples of blood serum: HIV-infected patients (n=128), pregnant women (n=86) and persons undergoing treatment or diagnostic examination (n=105). In the case of every infection agent analyzes were implemented using enzyme-linked immunosorbent assay and linear immunoblotting with new set and its analogues of German manufacturer. Read More

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January 2018

[The becoming of reactions of lipolysis in phylogenesis. The palmitic and oleic triglycerides as substrates. Insulin, condition of normolipemia and formation of hyper lipoproteinemia type IIb, IV and V].

Klin Lab Diagn 2018 ;63(1):4-15

The Federal state budget scientifc institution "The research institute of general pathology and pathophysiology" of the Russian academy of sciences, 125315, Moscow, Russia.

According to phylogenetic theory of general pathology, when living in ocean all were carnivorous (piscivorous) fatty acids transferring to cells in form of non-polar triglycerides nitially began apoB-48 chylomicrons, continued lipoproteins of very low and low density and fnalized its apoB-100 endocytosis. The fatty acids are transferred by chylomicrons + lipoproteins of very low density + lipoproteins of low density and non-polar triglycerides are hydrolyzed by hepatic glycerolhydrogenase and co-enzyme apoC-III; according WHO classifcation, hyperlipoproteinemia corresponds to type V. On land, in herbivorous who are not yet synthesized insulin, apoB-48 and chylomicrons left process of non-polar triglycerides transferring. Read More

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January 2018

Bayesian latent class analysis of ELISA and RT-rPCR diagnostic accuracy for subclinical Renibacterium salmoninarum infection in Atlantic salmon (Salmo salar) broodstock.

J Fish Dis 2018 Dec 14. Epub 2018 Dec 14.

Marine Harvest Canada, Campbell River, British Columbia, Canada.

Renibacterium salmoninarum infection causes bacterial kidney disease (BKD) in salmonid freshwater and saltwater life stages, with potentially severe financial loss for the aquaculture industry. Preventing vertical transmission, from infected broodstock to eggs, is key to disease management. As there is no perfect reference standard for detecting R. Read More

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December 2018

MicroRNAs: Unfolding the link between inositol-requiring enzyme 1α and fatty liver.

Hepatology 2018 Dec 14. Epub 2018 Dec 14.

Northwestern University Feinberg School of Medicine.

Nonalcoholic fatty liver disease (NAFLD) is currently the most common cause of abnormal liver chemistry tests in the United States and western world. The mechanisms underlying hepatic steatosis and the progression to nonalcoholic steatohepatitis (NASH) remain incompletely understood. Multiple cellular stress pathways are believed to contribute to the pathogenesis of fatty liver, including endoplasmic reticulum (ER) stress and its adaptive unfolded protein response (UPR). Read More

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December 2018

Guided cobamide biosynthesis for heterologous production of reductive dehalogenases.

Microb Biotechnol 2018 Dec 13. Epub 2018 Dec 13.

Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, Philosophenweg 12, D-07743, Jena, Germany.

Cobamides (Cbas) are essential cofactors of reductive dehalogenases (RDases) in organohalide-respiring bacteria (OHRB). Changes in the Cba structure can influence RDase function. Here, we report on the cofactor versatility or selectivity of Desulfitobacterium RDases produced either in the native organism or heterologously. Read More

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December 2018

Several Polyphosphate Kinase 2 Enzymes Produce Adenosine 5'-Polyphosphates.

Chembiochem 2018 Dec 14. Epub 2018 Dec 14.

GERMANY.

Polyphosphate kinases (PPK) are involved in many metabolic processes; enzymes of the second family (PPK2) are responsible for nucleotide synthesis under the consumption of inorganic polyphosphate. They phosphorylate nucleotides with a varying number of phosphate residues, such as monophosphates or diphosphates. Hence, these enzymes are promising candidates for cofactor regeneration systems. Read More

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December 2018

Facile formation of β-thioGlcNAc linkages to thiol-containing sugars, peptides, and proteins using a mutant GH20 hexosaminidase.

Angew Chem Int Ed Engl 2018 Dec 13. Epub 2018 Dec 13.

University of British Columbia, Chemistry, 2036 Main Mall, V6T 1Z1, Vancouver, CANADA.

Thioglycosides are hydrolase-resistant mimics of O-linked glycosides that can serve as valuable probes for studying the role of glycosides in biological processes. Traditional chemical syntheses of thioglycoside analogues of O-GlcNAc-modified peptides and proteins require lengthy, multi-step approaches. We report the development of efficient, enzyme-mediated syntheses of thioglycosides, including S-GlcNAcylated proteins, using a thioglycoligase derived from a GH20 hexosaminidase from Streptomyces plicatus in which the catalytic acid/base glutamate has been replaced with an alanine (SpHex E314A). Read More

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December 2018

Enkephalinase inhibitors, potential therapeutics for the future treatment of diarrhea predominant functional gastrointestinal disorders.

Neurogastroenterol Motil 2018 Dec 14:e13526. Epub 2018 Dec 14.

Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland.

The endogenous opioid system (EOS) is considered being a crucial element involved in the pathophysiology of irritable bowel syndrome (IBS) as it regulates gastrointestinal (GI) homeostasis through modulation of motility and water and ion secretion/absorption. Along with opioid receptors (ORs), the following components of EOS can be distinguished: 1. endogenous opioid peptides (EOPs), namely enkephalins, endorphins, endomorphins and dynorphins, and 2. Read More

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December 2018

Clinical value of enzyme immunoassay detecting rubella-specific immunoglobulin M immediately after the disease onset.

Microbiol Immunol 2018 Dec 14. Epub 2018 Dec 14.

National Hospital Organization, Nagoya Medical Center, 4-1-1 Sannomaru, Naka-ku, Nagoya, 460-0001, Japan.

A total of 300 nucleic acid test (NAT)-confirmed rubella patients, mostly adults, were examined to address clinical value of rubella specific IgM test using an enzyme immunoassay (EIA) kit. The IgM titer increased after rash onset, and the median IgM titer at 3 days post-onset was significantly higher than those at previous days (p < 0.0001). Read More

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December 2018

An assessment of the impact of green tea extract on palbociclib pharmacokinetics using a validated UHPLC-QTOF-MS method.

Biomed Chromatogr 2018 Dec 13:e4469. Epub 2018 Dec 13.

Drug Metabolism and Interactions Research Lab, Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, Telangana, India.

Green tea extracts (GTE) has been reported to be a kinase inhibitor and modulator for various drug metabolizing enzymes. It may give synergetic antioncogenic effect, but with a possibility of pharmacokinetic interactions with various co-administered anticancer agents like palbociclib (PAL), a selective inhibitor of CDK-4/6 primarily metabolized by CYP3A4 enzyme. To explore the impact of GTE on PAL pharmacokinetics in Sprague Dawley rats, a rapid and sensitive UHPLC-QTOF-MS method was established. Read More

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December 2018

The effect of carbohydrases or prebiotic oligosaccharides on growth performance, nutrient utilisation and the development of the small intestine and immune organs in broilers fed nutrient-adequate diets based on either wheat or barley.

J Sci Food Agric 2018 Dec 13. Epub 2018 Dec 13.

Scotland's Rural College, West Mains Road Edinburgh, EH9 3JG, UK.

Background: Non-starch polysaccharides (NSP) are large complex molecules and are found in cereal grains. This study was conducted to investigate the effect of carbohydrase enzymes or prebiotic oligosaccharides on growth performance, nutrient utilisation and the weight of organs associated with the immune system in broilers fed wheat or barley based diets.

Results: In wheat based diets, feed intake was lower following (P < 0. Read More

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December 2018

Wnt signaling reprograms metabolism in dental pulp stem cells.

J Cell Physiol 2018 Dec 13. Epub 2018 Dec 13.

Department of Cell Biology and Histology, University of the Basque Country (UPV/EHU), Barrio Sarriena, Leioa, Spain.

Human dental pulp stem cells (DPSCs) can differentiate to a wide range of different cell lineages, and share some gene expression and functional similarities with pluripotent stem cells. The stemness of DPSCs can also be pharmacologically enhanced by the activation of canonical Wnt signaling. Here, we examined the metabolic profile of DPSCs during reprogramming linked to Wnt activation, by a short (48 hr) exposure to either the GSK3-β inhibitor BIO (6-bromoindirubin-3´-oxine) or human recombinant protein WNT-3A. Read More

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December 2018

New treatment method for mucopolysaccharidosis type VI by liver transplantation.

Pediatr Int 2018 Dec 13. Epub 2018 Dec 13.

Department of Clinical Diagnosis, National Center for Child Health and Development, Tokyo.

Background: Mucopolysaccharidosis (MPS) VI is a rare, autosomal recessive congenital metabolic disorder caused by deficient activity of the lysosomal metabolic enzyme, N-acetylgalactosamine 4-sulfatase. Enzyme replacement therapy (ERT) is current therapeutic treatment for MPS VI, while involves limited compliance to the therapy and high costs. This study aimed to develop a new method of treatment by conducting an orthotopic liver transplantation using an animal model of human MPS VI, and to evaluate and examine its effectiveness for treating MPS VI. Read More

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December 2018

LC/MS detection of oligogalacturonic acids obtained from tragacanth degradation by pectinase producing bacteria.

J Basic Microbiol 2018 Dec 13. Epub 2018 Dec 13.

School of Chemical and Biological Engineering, Seoul National University, Gwanak-gu, Seoul, Republic of Korea.

Tragacanth, a highly branched carbohydrate polymer isolated from Astragalus, is one of the most commonly used gums in food industry. The primary structure of tragacanth is composed of galacturonic acid monomers connected with α 1-4 links, and it is very similar to the pectin. Tragacanth degradation by microorganisms is significant in two aspects: first, food preservation and microbial growth control due to too much use of tragacanth in the food industry, second, therapeutic and pharmaceutical potential of obtained oligosaccharides. Read More

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December 2018

Hepatocyte Injury Induced by Contrast-Enhanced Diagnostic Ultrasound.

J Ultrasound Med 2018 Dec 13. Epub 2018 Dec 13.

Department of Radiology, University of Michigan Health System, Ann Arbor, Michigan, USA.

Objectives: Contrast-enhanced diagnostic ultrasound (US) has a potential to induce localized biological effects. The potential for contrast-enhanced diagnostic US bioeffects in liver were researched, with guidance from a report by Yang et al (Ultrasonics 2012; 52:1065-1071).

Methods: Contact and standoff scanning was performed for 10 minutes with a diagnostic US phased array at 1. Read More

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December 2018

Production and characterization of β-cyclodextrin glucanotransferase from Bacillus sp. ND1.

J Basic Microbiol 2018 Dec 13. Epub 2018 Dec 13.

School of Life Sciences, Jaipur National University, Jaipur, Rajasthan, India.

A potent β-CGTase producing bacterium ND1 has been isolated from sugarcane field soil in India. The biochemical, physiologicaland phylogenetic analyses based on 16S rRNA gene suggest that the isolate belongs to Bacillus cereus group. The enzyme β-CGTase produced from isolate ND1 catalyzes production of β-cyclodextrin utilizing starch as a substrate which has diverse applications in various fields. Read More

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December 2018
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The construction of an in vitro synthetic enzymatic biosystem that facilitates laminaribiose biosynthesis from maltodextrin and glucose.

Biotechnol J 2018 Dec 13:e1800493. Epub 2018 Dec 13.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, People's Republic of China.

Laminaribiose is a reducing glucobiose linked by a β-1,3 glycosidic bond; it is also a precursor for building blocks in the pharmaceutical industry, a powerful germinating agent and antiseptic, as well as a potential prebiotic. In this study, an in vitro enzymatic biosystem composed of α-glucan phosphorylase, laminaribiose phosphorylase, isoamylase and 4-glucanotransferase was designed for the one-pot synthesis of laminaribiose from low-cost maltodextrin and glucose. Through condition optimization, 51 mM laminaribiose was produced from 10 g/L maltodextrin (55. Read More

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December 2018

Clinical indices and local levels of inflammatory biomarkers in per-implant health of obese and nonobese individuals.

Clin Implant Dent Relat Res 2018 Dec 13. Epub 2018 Dec 13.

Department of Periodontology, Faculty of Dentistry, Ziauddin University, Karachi, Pakistan.

Background: Obesity seem to regulate peri-implant health. It is proposed that peri-implant crevicular fluid (PICF) levels of interleukin (IL)-1β and IL-6 are higher in obese as compared to nonobese individuals.

Objective: The purpose of the present clinico-laboratory study is to estimate and compare the clinical and radiographic indices and PICF levels of IL-1β and IL-6 among obese and nonobese patients. Read More

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December 2018

The effect of early growth response 1 on levels of Amyloid-β 40 peptide in U87MG cells.

J Cell Biochem 2018 Dec 12. Epub 2018 Dec 12.

Department of Neurology, The Frist Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

A recent study has shown that early growth response 1 (EGR1) plays a critical role in the β-amyloid cascade and tau hypotheses. In addition, evidence has suggested that EGR1 can regulate levels of amyloid-beta peptides, key molecules in the pathogenesis of Alzheimer's disease (AD). However, whether EGR1 is a deleterious or protective factor in the AD is still controversial. Read More

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December 2018

Asperyellone prevents HDF cells from UVB irradiation damages: An elaborated study.

J Cell Biochem 2018 Dec 12. Epub 2018 Dec 12.

Microbiology Division, CSIR-CLRI, Chennai, Tamil Nadu, India.

The current study explores the photo-protective effect of asperyellone (AY) (a fungal secondary metabolite), assessed under in vitro condition using human dermal fibroblast cell line. AY was isolated from Aspergillus sp. during the resting phase and purified. Read More

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December 2018
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Methylene blue regulates inflammatory response in osteoarthritis by noncoding long chain RNA CILinc02.

J Cell Biochem 2018 Dec 12. Epub 2018 Dec 12.

The Second Department of Orthopedics, Zhang Zhou Zhengxing Hospital, Zhangzhou, China.

Long noncoding RNAs serve as novel regulatory molecules involved in various biological processes, but their role in osteoarthritis (OA) remains unknown. Therefore, we aimed to reveal the inflammatory mechanisms regulated by CILinc02 and methylene blue in human osteoarthritic cartilage and to explore the potential functions of CILinc02 and methylene blue in OA. The expression level of CILinc02 in OA cartilage tissues and primary cells was obtained using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Read More

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December 2018

Secondary structure analysis of peptides with relevance to iron-sulfur cluster nesting.

J Comput Chem 2019 Jan;40(2):515-526

Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, 59718.

Peptides coordinated to iron-sulfur clusters, referred to as maquettes, represent a synthetic strategy for constructing biomimetic models of iron-sulfur metalloproteins. These maquettes have been successfully employed as building blocks of engineered heme-containing proteins with electron-transfer functionality; however, they have yet to be explored in reactivity studies. The concept of iron-sulfur nesting in peptides is a leading hypothesis in Origins-of-Life research as a plausible path to bridge the discontinuity between prebiotic chemical transformations and extant enzyme catalysis. Read More

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January 2019
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