2,620 results match your criteria Environmental and Molecular Mutagenesis[Journal]


Comparative Mutagenic Activity of Atmospheric Particulate Matter From Limeira, Stockholm and Kyoto.

Environ Mol Mutagen 2019 Apr 9. Epub 2019 Apr 9.

Pharmaceutical Sciences Faculty, University of São Paulo (USP), São Paulo, SP, Brazil.

Atmospheric particulate matter (PM) organic fraction from urban centers are frequently mutagenic for the Salmonella/microsome assay. This mutagenicity is related to both primary and secondary pollutants, and meteorological conditions have great influence in the secondary pollutant's formation. Our objective was to compare the mutagenicity of atmospheric total suspended particulates (TSP) from three cities with marked different meteorological conditions and TSP concentrations: Limeira (Brazil), with 99. Read More

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http://dx.doi.org/10.1002/em.22293DOI Listing

Introduction: Special Issue on Transplacental/Transgenerational Mutagenesis and Carcinogenesis.

Environ Mol Mutagen 2019 Apr 5. Epub 2019 Apr 5.

Intramural Diversity Workforce Branch (IDWB), Center for Cancer Training, National Cancer Institute, National Institutes of Health, 9609 Medical Center Drive, Rockville, MD 20850.

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http://dx.doi.org/10.1002/em.22292DOI Listing

In vitro effects of 1-MHz ultrasound on the mitotic spindle.

Environ Mol Mutagen 2019 Mar 14. Epub 2019 Mar 14.

Dipartimento di Scienze, Università Roma Tre, Rome, Italy.

The effects of ultrasound on the cytoskeleton, comprising microtubules, had been studied decades ago. Nonetheless, very little attention has been paid to the effects of ultrasound on the mitotic spindle, which is also formed by microtubules. In this study, we treated human fibroblasts and human cancer cells (HeLa and MCF-7) with 1-MHz ultrasound at low intensities (70, 140, and 300 mW/cm ). Read More

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http://dx.doi.org/10.1002/em.22287DOI Listing
March 2019
1 Read

The respiratory tract microbiome and its relationship to lung cancer and environmental exposures found in rural China.

Environ Mol Mutagen 2019 Apr 3. Epub 2019 Apr 3.

Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA.

We previously reported that bacterial diversity in sputum samples from never smoking women in rural China varied by lung cancer status and household air pollution (HAP) exposure type. Here we expand upon our associations between environmental exposures and respiratory tract microbiota with an additional 90 never smoking women from Xuanwei, China. DNA from sputum samples of cases (n=45) and controls (n=45) was extracted using a multi-step enzymatic and physical lysis, followed by a standardized clean-up. Read More

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http://dx.doi.org/10.1002/em.22291DOI Listing

ImuB and ImuC contribute to UV-induced mutagenesis as part of the SOS regulon in Pseudomonas aeruginosa.

Environ Mol Mutagen 2019 Mar 28. Epub 2019 Mar 28.

Universidad Nacional de Córdoba. Facultad de Ciencias Químicas., Departamento de Química Biológica Ranwel Caputto., Córdoba, Argentina.

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http://dx.doi.org/10.1002/em.22290DOI Listing

Transplacental Carcinogenesis Induced by Antiretrovirals, Twelve Years Later.

Authors:
Ofelia A Olivero

Environ Mol Mutagen 2019 Mar 28. Epub 2019 Mar 28.

Intramural Diversity Workforce Branch, Center for Cancer Training, National Cancer Institute, NIH, Rockville, Maryland.

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http://dx.doi.org/10.1002/em.22289DOI Listing

Pregnancy drugs, fetal germline epigenome, and risks for next-generation pathology: A call to action.

Environ Mol Mutagen 2019 Mar 19. Epub 2019 Mar 19.

DES Action USA, New York, New York.

Drugs taken during pregnancy can affect three generations at once: the gestating woman (F0), her exposed fetus (F1), and the fetal germ cells that confer heritable information for the grandchildren (F2). Unfortunately, despite growing evidence for connections between F0 drug exposures and F2 pathology, current approaches to risk assessment overlook this important dimension of risk. In this commentary, we argue that the unique molecular vulnerabilities of the fetal germline, particularly with regard to global epigenomic reprogramming, combined with empirical evidence for F2 effects of F1 in utero drug and other exposures, should change the way we consider potential long-term consequences of pregnancy drugs and alter toxicology's standard somatic paradigm. Read More

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http://dx.doi.org/10.1002/em.22288DOI Listing
March 2019
1 Read

Genomic chaos in peripheral blood lymphocytes of hodgkins lymphoma patients 1 year after ABVD chemotherapy/radiotherapy.

Authors:
Sara Frias

Environ Mol Mutagen 2019 05;60(4):387-388

Laboratorio de Citogenética, INP, Insurgentes Sur 3700-C, 6o. piso, Cd. De Mexico, Mexico.

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http://dx.doi.org/10.1002/em.22279DOI Listing
May 2019
1 Read

In vivo Characterization of the Radiosensitizing Effect of a Very Low Dose of BrdU in Murine Cells Exposed to Low-Dose Radiation.

Environ Mol Mutagen 2019 Mar 9. Epub 2019 Mar 9.

Instituto Nacional de Investigaciones Nucleares, Carretera México-Toluca s/n, La Marquesa, Ocoyoacac, Estado de México C. P., 52750, Mexico.

The aim of the present study was to characterize the in vivo radiosensitizing effect of a very low dose of bromodeoxyuridine (BrdU) in mice exposed to low-dose radiation by establishing the following: (1) the radiosensitizing effect during DNA synthesis using single-cell gel electrophoresis (SCGE) in murine bone marrow cells, and (2) the number and timing of the mechanisms of genotoxicity and cytotoxicity, as well as the correlation of both end points, using flow cytometry analysis of the kinetics of micronucleus induction in reticulocytes. Groups of mice received intraperitoneal injections of 0.125 mg/g of BrdU 24 h prior to irradiation with 0. Read More

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http://dx.doi.org/10.1002/em.22284DOI Listing
March 2019
1 Read

Letter to the editor of environmental and molecular mutagenesis: In regard to Ramos et al.

Environ Mol Mutagen 2019 05;60(4):385-386

Department of Medicine, Gustave Roussy Cancer Campus, University Paris-Saclay, Villejuif, France.

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http://dx.doi.org/10.1002/em.22282DOI Listing
May 2019
2 Reads

Exploring the interaction between FGF Genes and T-box genes among chinese nonsyndromic cleft lip with or without cleft palate case-parent trios.

Environ Mol Mutagen 2019 Mar 8. Epub 2019 Mar 8.

School of Public Health, Johns Hopkins University, Baltimore, Maryland.

Nonsyndromic cleft lip with or without cleft palate (NSCL/P) is a common birth defect. Genetic variants causing syndromic orofacial clefts can also contribute to the etiology of NSCL/P. The purpose of the present study was to explore gene-gene (G × G) interaction using common single nucleotide polymorphic (SNP) markers in fibroblast growth factor (FGF) family and its receptors and T-box genes, which were associated with syndromic orofacial clefts. Read More

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http://dx.doi.org/10.1002/em.22286DOI Listing
March 2019
1 Read
2.630 Impact Factor

Genome-Wide DNA Methylation Changes in Transformed Foci Induced by Nongenotoxic Carcinogens.

Environ Mol Mutagen 2019 Mar 8. Epub 2019 Mar 8.

Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of Korea.

In vitro cell transformation assays (CTA) have been proposed as a method to identify possible nongenotoxic carcinogens. However, the current protocols do not provide information on the mechanism of action of the test articles. In this study, we combined an in vitro Bhas 42 CTA and sequencing-based DNA methylation profiling analysis to elucidate the carcinogenic mechanism associated with nongenotoxic carcinogens. Read More

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http://dx.doi.org/10.1002/em.22285DOI Listing
March 2019
5 Reads

Longitudinal study of t-cell somatic mutations conferring glycosylphosphatidylinositol-anchor deficiency in gulf war I veterans exposed to depleted uranium.

Environ Mol Mutagen 2019 Mar 8. Epub 2019 Mar 8.

Occupational Health Program, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland.

Fifty Veterans of the first Gulf War in 1991 exposed to depleted uranium (DU) were studied for glycosylphosphatidylinositol-anchor (GPIa) deficient T-cell mutants on three occasions during the years 2009, 2011, and 2013. GPIa deficiency was determined in two ways: cloning assays employing aerolysin selection and cytometry using the FLAER reagent for positive staining of GPIa cell surface proteins. Subsequent molecular analyses of deficient isolates recovered from cloning assays (Nicklas JA et al. Read More

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http://dx.doi.org/10.1002/em.22281DOI Listing
March 2019
1 Read

Genetic instability of in vitro cell lines: Implications for genetic toxicity testing.

Environ Mol Mutagen 2019 Mar 8. Epub 2019 Mar 8.

Toxicology & Environmental Research and Consulting, The Dow Chemical Company, Midland, Michigan.

Cell line-based in vitro testing has been widely used as an important component of the genotoxicity testing battery; however, the use of cell lines is constrained by several limitations, including the genetic drift and variability. A study recently reported in the literature comprehensively examined genomic changes in a large number of cell lines and reported extensive genetic variations within the same cell lines across passage numbers and laboratories, even for single-cell derived subclones. The primary objective of this communication is to raise awareness and stimulate discussion within the genotoxicity testing community of the extent of genetic variability of cell lines in general and how these variables could potentially influence the results and reproducibility of genotoxicity testing. Read More

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http://dx.doi.org/10.1002/em.22280DOI Listing
March 2019
1 Read

Molecular analysis of glycosylphosphatidylinositol anchor deficient aerolysin resistant isolates in gulf war i veterans exposed to depleted uranium.

Environ Mol Mutagen 2019 Mar 8. Epub 2019 Mar 8.

Department of Pathology, University of Vermont College of Medicine, Burlington, Vermont.

During the First Gulf War (1991) over 100 servicemen sustained depleted uranium (DU) exposure through wound contamination, inhalation, and shrapnel. The Department of Veterans Affairs has a surveillance program for these Veterans which has included genotoxicity assays. The frequencies of glycosylphosphatidylinositol anchor (GPIa) negative (aerolysin resistant) cells determined by cloning assays for these Veterans are reported in Albertini RJ et al. Read More

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http://doi.wiley.com/10.1002/em.22283
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http://dx.doi.org/10.1002/em.22283DOI Listing
March 2019
16 Reads

Critical review of styrene genotoxicity focused on the mutagenicity/clastogenicity literature and using current organization of economic cooperation and development guidance.

Environ Mol Mutagen 2019 Feb 20. Epub 2019 Feb 20.

Ramboll, 124 West Capitol Avenue, Suite 1605, Little Rock, Arkansas.

Styrene is an important high production volume chemical used to manufacture polymeric products. In 2018, International Agency for Research on Cancer classified styrene as probably carcinogenic to humans; National Toxicology Program lists styrene as reasonably anticipated to be a human carcinogen. The genotoxicity literature for styrene and its primary metabolite, styrene 7,8-oxide (SO), begins in the 1970s. Read More

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http://dx.doi.org/10.1002/em.22278DOI Listing
February 2019
1 Read

The development and prevalidation of an in vitro mutagenicity assay based on MutaMouse primary hepatocytes, Part II: Assay performance for the identification of mutagenic chemicals.

Environ Mol Mutagen 2019 05 25;60(4):348-360. Epub 2019 Feb 25.

Environmental Health Science and Research Bureau, Health Canada, Ottawa, Ontario, Canada.

As demonstrated in Part I, cultured MutaMouse primary hepatocytes (PHs) are suitable cells for use in an in vitro gene mutation assay due to their metabolic competence, their "normal" phenotype, and the presence of the MutaMouse transgene for reliable mutation scoring. The performance of these cells in an in vitro gene mutation assay is evaluated in this study, Part II. A panel of 13 mutagenic and nonmutagenic compounds was selected to investigate the performance of the MutaMouse PH in vitro gene mutation assay. Read More

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http://dx.doi.org/10.1002/em.22277DOI Listing
May 2019
1 Read

Prenatal exposure to particulate matter and ozone: Bulky DNA adducts, plasma isoprostanes, allele risk variants, and neonate susceptibility in the Mexico City Metropolitan Area.

Environ Mol Mutagen 2019 Jan 31. Epub 2019 Jan 31.

Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de México, México.

Mexico City's Metropolitan Area (MCMA) includes Mexico City and 60 municipalities of the neighbor states. Inhabitants are exposed to emissions from over five million vehicles and stationary sources of air pollutants such as particulate matter (PM) and ozone. MCMA PM contains elemental carbon and organic carbon (OC). Read More

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http://doi.wiley.com/10.1002/em.22276
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http://dx.doi.org/10.1002/em.22276DOI Listing
January 2019
7 Reads

Particulate matter-associated micronuclei frequencies in maternal and cord blood lymphocytes.

Environ Mol Mutagen 2019 Jan 31. Epub 2019 Jan 31.

Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de México, Mexico.

Studies associate particulate matter (PM) exposure with pulmonary, cardiovascular, and neurologic diseases. Elevated levels of coarse (PM10) and fine (PM2.5) PM have been reported in the Mexico City metropolitan area during the last two decades. Read More

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http://dx.doi.org/10.1002/em.22275DOI Listing
January 2019
5 Reads

Predictions of genotoxic potential, mode of action, molecular targets, and potency via a tiered multiflow® assay data analysis strategy.

Environ Mol Mutagen 2019 Jan 31. Epub 2019 Jan 31.

Institute of Life Science, Swansea University Medical School, Swansea University, Swansea, Wales, United Kingdom.

The in vitro MultiFlow® DNA Damage Assay multiplexes γH2AX, p53, phospho-histone H3, and polyploidization biomarkers into a single flow cytometric analysis. The current report describes a tiered sequential data analysis strategy based on data generated from exposure of human TK6 cells to a previously described 85 chemical training set and a new pharmaceutical-centric test set (n = 40). In each case, exposure was continuous over a range of closely spaced concentrations, and cell aliquots were removed for analysis following 4 and 24 hr of treatment. Read More

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http://doi.wiley.com/10.1002/em.22274
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http://dx.doi.org/10.1002/em.22274DOI Listing
January 2019
22 Reads
2.630 Impact Factor

Expression levels of circulating microRNAs-126, -155, and -145 in Mexican women exposed to polycyclic aromatic hydrocarbons through biomass fuel use.

Environ Mol Mutagen 2019 Jan 30. Epub 2019 Jan 30.

Laboratorio de Toxicología Molecular, Centro de Investigación Aplicada en Ambiente y Salud (CIAAS), Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luis Potosí., San Luis Potosí, Mexico.

Human exposure to polycyclic aromatic hydrocarbons (PAHs) has been considered a risk determinant for the development of cardiovascular diseases (CVD). Therefore, the aim of this study was to assess expression levels of vascular-related miRNAs, miR-126, miR-155, and miR-145, in plasma from women (aged 19-81 years) exposed (n = 100) and non-exposed (n = 20) to PAHs via biomass combustion smoke.1-hydroxypyrene (1-OHP) was determined in urine as a biomarker of exposure to PAHs using high-resolution liquid chromatography. Read More

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http://dx.doi.org/10.1002/em.22273DOI Listing
January 2019

DNA polymerase kappa counteracts inflammation-induced mutagenesis in multiple organs of mice.

Environ Mol Mutagen 2019 05 21;60(4):320-330. Epub 2019 Jan 21.

Biological Safety Research Center, National Institute of Health Sciences, Kawasaki-ku, Kawasaki-shi, Kanagawa, Japan.

In vitro studies indicate that DNA polymerase kappa (Polκ) is able to accurately and efficiently perform DNA synthesis using templates containing various types of DNA damage, including benzo[a]pyrene (BP)-induced N -deoxyguanosine adducts. In this study, we examined sensitivity of inactivated Polk knock-in (Polk ) mice to BP carcinogenicity in the colon by administering an oral dose of BP plus dextran sulfate sodium (DSS), an inflammation causing promoter of carcinogenesis. Although colon cancer was successfully induced by BP plus DSS, there was no significant difference in tumor incidence or multiplicity between Polk and Polk mice. Read More

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http://doi.wiley.com/10.1002/em.22272
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http://dx.doi.org/10.1002/em.22272DOI Listing
May 2019
6 Reads

Integrated In Vivo Genotoxicity Assessment of Procarbazine Hydrochloride Demonstrates Induction of Pig-a and LacZ Mutations, and Micronuclei, in MutaMouse Hematopoietic Cells.

Environ Mol Mutagen 2018 Dec 28. Epub 2018 Dec 28.

Environmental Health Science and Research Bureau, Health Canada, Ottawa, Ontario, Canada.

Procarbazine hydrochloride (PCH) is a DNA-reactive hematopoietic carcinogen with potent and well-characterized clastogenic activity. However, there is a paucity of in vivo mutagenesis data for PCH, and in vitro assays often fail to detect the genotoxic effects of PCH due to the complexity of its metabolic activation. We comprehensively evaluated the in vivo genotoxicity of PCH on hematopoietic cells of male MutaMouse transgenic rodents using a study design that facilitated assessments of micronuclei and Pig-a mutation in circulating erythrocytes, and lacZ mutant frequencies in bone marrow. Read More

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http://dx.doi.org/10.1002/em.22271DOI Listing
December 2018
3 Reads

The development and prevalidation of an in vitro mutagenicity assay based on MutaMouse primary hepatocytes, Part I: Isolation, structural, genetic, and biochemical characterization.

Environ Mol Mutagen 2019 05 27;60(4):331-347. Epub 2018 Dec 27.

Environmental Health Science and Research Bureau, Health Canada, Ottawa, Ontario, Canada.

To develop an improved in vitro mammalian cell gene mutation assay, it is imperative to address the known deficiencies associated with existing assays. Primary hepatocytes isolated from the MutaMouse are ideal for an in vitro gene mutation assay due to their metabolic competence, their "normal" karyotype (i.e. Read More

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http://doi.wiley.com/10.1002/em.22253
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http://dx.doi.org/10.1002/em.22253DOI Listing
May 2019
4 Reads

Relative telomere length and gene expression of shelterin complex proteins among vinyl chloride monomer-exposed workers in China.

Environ Mol Mutagen 2019 05 13;60(4):361-367. Epub 2019 Jan 13.

Department of Occupational Health and Toxicology, School of Public Health, Fudan University, and Key Laboratory of Public Health and Safety of Ministry of Education of China, 138 Yixueyuan Road, Shanghai, 200032, China.

Vinyl chloride monomer (VCM) is a confirmed carcinogen. The effects of VCM on telomeres and the gene expression of telomere complex proteins, shelterin, have not been well studied but could be of potential relevance to the carcinogenic mechanism of VCM and the health surveillance of VCM-exposed workers. A group of 241 VCM-exposed workers and 101 internal controls from the same plant in Shandong, China were recruited and quantitative polymerase chain reaction was preformed to measure relative telomere length (RTL) and gene expression of shelterin proteins. Read More

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http://dx.doi.org/10.1002/em.22270DOI Listing
May 2019
3 Reads

Differentiation of Aneugens and Clastogens in the In Vitro Micronucleus Test by Kinetochore Scoring Using Automated Image Analysis.

Environ Mol Mutagen 2019 04 18;60(3):227-242. Epub 2018 Dec 18.

Bayer AG, Investigational Toxicology, Berlin, Germany.

The in vitro micronucleus test according to OECD Test Guideline 487 (TG 487) is widely used to investigate the genotoxic potential of drugs. Besides the identification of in vitro genotoxicants, the assay can be complemented with kinetochore staining for the differentiation between clastogens and aneugens. This differentiation constitutes a major contribution to risk assessment as especially aneugens show a threshold response. Read More

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http://dx.doi.org/10.1002/em.22259DOI Listing
April 2019
2 Reads

JAK2 regulates mismatch repair protein-mediated epigenetic alterations in response to oxidative damage.

Environ Mol Mutagen 2019 05 7;60(4):308-319. Epub 2019 Jan 7.

Medical Sciences, Indiana University School of Medicine, Bloomington, Indiana.

At sites of chronic inflammation epithelial cells undergo aberrant DNA methylation that contributes to tumorigenesis. Inflammation is associated with an increase in reactive oxygen species (ROS) that cause oxidative DNA damage, which has also been linked to epigenetic alterations. We previously demonstrated that in response to ROS, mismatch repair proteins MSH2 and MSH6 recruit epigenetic silencing proteins DNA methyltransferase 1 (DNMT1) and polycomb repressive complex 2 (PRC2) members to sites of DNA damage, resulting in transcriptional repression of tumor suppressor genes (TSGs). Read More

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http://dx.doi.org/10.1002/em.22269DOI Listing
May 2019
2 Reads

Is chronic use of hydroxyurea safe for patients with sickle cell anemia? An account of genotoxicity and mutagenicity.

Environ Mol Mutagen 2019 04 7;60(3):302-304. Epub 2018 Dec 7.

Faculty of Pharmacy, Federal University of Ceará, Fortaleza, Brazil.

Sickle cell anemia (SCA) is a hereditary hematological disease that is characterized by a point mutation in the beta globin S gene and has no specific treatment; hydroxyurea (HU) is the only therapeutic agent used in clinical practice. In the present study, we evaluated the deoxyribonucleic acid (DNA) damage index (DI) and chromosomal damage in leukocytes of adult patients with SCA with and without HU. The DI was assessed by the comet assay and chromosomal damage by the leukocyte micronucleus test of adult patients treated with HU (SCA-HU) and without the use of HU (SCA-NoHU). Read More

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http://dx.doi.org/10.1002/em.22260DOI Listing
April 2019
16 Reads

Understanding the importance of low-molecular weight (ethylene oxide- and propylene oxide-induced) DNA adducts and mutations in risk assessment: Insights from 15 years of research and collaborative discussions.

Environ Mol Mutagen 2019 03 10;60(2):100-121. Epub 2018 Dec 10.

University of North Carolina, Chapel Hill, North Carolina, USA.

The interpretation and significance of DNA adduct data, their causal relationship to mutations, and their role in risk assessment have been debated for many years. An extended effort to identify key questions and collect relevant data to address them was focused on the ubiquitous low MW N7-alkyl/hydroxyalkylguanine adducts. Several academic, governmental, and industrial laboratories collaborated to gather new data aimed at better understanding the role and potential impact of these adducts in quantifiable genotoxic events (gene mutations/micronucleus). Read More

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http://dx.doi.org/10.1002/em.22248DOI Listing
March 2019
3 Reads

Effects of Acute Fluorene-9-Bisphenol Exposure on Mouse Oocyte in vitro Maturation and Its Possible Mechanisms.

Environ Mol Mutagen 2019 04 30;60(3):243-253. Epub 2018 Nov 30.

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.

Fluorene-9-bisphenol (BHPF), a substitute of bisphenol A (BPA) used in the production of the so-called "BPA-free" plastics, has now been shown to be released from commercial plastic bottles into drinking water and has strong anti-estrogenic activity in mice, which suggests that BHPF is also an environmental toxin. However, whether BHPF exposure has effects on mouse oocyte development is unknown. In this study, the influence of acute exposure to BHPF (50-150 μM, 12 hr) on mouse oocyte maturation and its possible mechanisms were investigated. Read More

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http://dx.doi.org/10.1002/em.22258DOI Listing
April 2019
3 Reads
2.630 Impact Factor

LINE-1 hypomethylation is associated with radiation-induced genomic instability in industrial radiographers.

Environ Mol Mutagen 2019 03 29;60(2):174-184. Epub 2018 Nov 29.

School of Public Health, Seoul National University, Seoul, South Korea.

Global DNA hypomethylation is proposed as a potential biomarker for cancer risk associated with genomic instability, which is an important factor in radiation-induced cancer. However, the associations among radiation exposure, changes in DNA methylation, and carcinogenesis are unclear. The aims of this study were (1) to examine whether low-level occupational radiation exposure induces genomic DNA hypomethylation; and (2) to determine the relationships between radiation exposure, genomic DNA hypomethylation and radiation-induced genomic instability (RIGI) in industrial radiographers. Read More

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http://dx.doi.org/10.1002/em.22237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363886PMC
March 2019
8 Reads

Assessment of the performance of the TGx-DDI biomarker to detect DNA damage-inducing agents using quantitative RT-PCR in TK6 cells.

Environ Mol Mutagen 2019 03 29;60(2):122-133. Epub 2018 Nov 29.

Environmental Health Science and Research Bureau, Health Canada, Ottawa, Ontario, Canada.

Gene expression biomarkers are now available for application in the identification of genotoxic hazards. The TGx-DDI transcriptomic biomarker can accurately distinguish DNA damage-inducing (DDI) from non-DDI exposures based on changes in the expression of 64 biomarker genes. The 64 genes were previously derived from whole transcriptome DNA microarray profiles of 28 reference agents (14 DDI and 14 non-DDI) after 4 h treatments of TK6 human lymphoblastoid cells. Read More

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http://dx.doi.org/10.1002/em.22257DOI Listing
March 2019
4 Reads

Mutagenicity of Ayahuasca and Their Constituents to the Salmonella/Microsome Assay.

Environ Mol Mutagen 2019 04 29;60(3):269-276. Epub 2018 Nov 29.

Department of Physiological Sciences, Santa Casa de São Paulo School of Medical Sciences, São Paulo, Brazil.

Ayahuasca is a beverage used in religious rituals of indigenous and nonindigenous groups, and its therapeutic potential has been investigated. Ayahuasca is obtained by decoction of the Banisteriopsis caapi that contains β-carbolines (harmine, harmaline, and tetrahydroharmine) plus Psychotria viridis that contains N,N-dimethyltryptamine. Although plants used in folk medicine are recognized as safe, many of them have genotoxic potential. Read More

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http://doi.wiley.com/10.1002/em.22263
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http://dx.doi.org/10.1002/em.22263DOI Listing
April 2019
7 Reads

Leukocyte Telomere Length in Patients with Radiographic Knee Osteoarthritis.

Environ Mol Mutagen 2019 04 29;60(3):298-301. Epub 2018 Nov 29.

INIBIC-Complexo Hospitalario Universitario A Coruña (CHUAC), Genetics Unit, A Coruña, Spain.

Relative mean telomere sequence amount was determined by quantitative PCR (qPCR) of peripheral blood leukocyte (PBL) samples obtained at recruitment (n = 310) from individuals from the Osteoarthritis (OA) Initiative consortium. Knees were radiologically evaluated according to the Kellgren-Lawrence (KL) score, ranging from 0 to 4, considering a KL grade ≥ 2 as radiographic evidence of OA (n = 124). Telomere size decreased as baseline KL score increased, being significantly shorter in subjects with KL ≥2 (Mann-Whitney U-test, P < 0. Read More

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http://dx.doi.org/10.1002/em.22247DOI Listing
April 2019
18 Reads

Inhalation exposure to low levels of ethyl tertiary butyl ether: Its genetic effects were significantly modified by ALDH2 activity.

Environ Mol Mutagen 2019 03 25;60(2):145-153. Epub 2018 Nov 25.

National Institute of Occupational Safety and Health, Kawasaki, Japan.

Previous experiments showed that high concentrations of ethyl tertiary butyl ether (ETBE) exposure (500-5,000 ppm) significantly resulted in DNA damages in aldehyde dehydrogenase 2 (Aldh2) knockout (KO) mice. This study was aimed to verify the genotoxic effects in three genetic types, Aldh2 KO, heterogeneous (HT), and wild type (WT), of mice exposed to lower concentrations of ETBE (50-500 ppm) by inhalation. Histopathology assessments in the livers, measurements of genotoxic biomarkers in blood and livers, and urinary 8-hydroxydeoxyguanosion (8-OH-dG) for the oxidative DNA damage of whole body were performed. Read More

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http://dx.doi.org/10.1002/em.22256DOI Listing
March 2019
1 Read

Exposure to the Fungicide Captan Induces DNA Base Alterations and Replicative Stress in Mammalian Cells.

Environ Mol Mutagen 2019 04 19;60(3):286-297. Epub 2018 Dec 19.

Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse, France.

The classification of the fungicide captan (CAS Number: 133-06-2) as a carcinogen agent is presently under discussion. Despite the mutagenic effect detected by the Ames test and carcinogenic properties observed in mice, the genotoxicity of this pesticide in humans is still unclear. New information is needed about its mechanism of action in mammalian cells. Read More

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http://dx.doi.org/10.1002/em.22268DOI Listing
April 2019
18 Reads
2.630 Impact Factor

The SOS system: A complex and tightly regulated response to DNA damage.

Environ Mol Mutagen 2019 05 7;60(4):368-384. Epub 2019 Jan 7.

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.

Genomes of all living organisms are constantly threatened by endogenous and exogenous agents that challenge the chemical integrity of DNA. Most bacteria have evolved a coordinated response to DNA damage. In Escherichia coli, this inducible system is termed the SOS response. Read More

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http://doi.wiley.com/10.1002/em.22267
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http://dx.doi.org/10.1002/em.22267DOI Listing
May 2019
19 Reads

Cytome micronucleus assays with a metabolically competent human derived liver cell line (Huh6): A promising approach for routine testing of chemicals?

Environ Mol Mutagen 2019 03 8;60(2):134-144. Epub 2018 Nov 8.

Institute of Cancer Research, Department of Internal Medicine 1, Medical University of Vienna, Vienna, Austria.

One of the main problems of in vitro genotoxicity tests is the inadequate representation of drug metabolizing enzymes in most indicator cell lines which are currently used. We identified recently a human derived liver cell line (Huh6) which detected induction of DNA damage by representatives of different groups of promutagens without enzyme mix and showed that these cells are more suitable in terms of reproducibility and sensitivity as other currently used liver derived lines. We developed a protocol for micronucleus (MN) cytome assays with these cells and validated the procedure in experiments with representatives of different groups of directly and indirectly acting genotoxic carcinogens (MMS, cisplatin, PhIP, IQ, NDMA, B(a)P, AFB1, etoposide, and H O ). Read More

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http://doi.wiley.com/10.1002/em.22254
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http://dx.doi.org/10.1002/em.22254DOI Listing
March 2019
30 Reads

Geraniin Differentially Modulates Chromosome Stability of Colon Cancer and Noncancerous Cells by Oppositely Regulating their Spindle Assembly Checkpoint.

Environ Mol Mutagen 2019 04 26;60(3):254-268. Epub 2018 Dec 26.

School of Life Sciences, The Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Yunnan Normal University, Kunming, Yunnan, China.

Geraniin has been reported to specifically induce apoptosis in multiple human cancers, but the underlying mechanism is poorly defined. The spindle assembly checkpoint (SAC) is a surveillance system to ensure high-fidelity chromosome segregation during mitosis. Weakening of SAC to enhance chromosome instability (CIN) can be therapeutic because very high levels of CIN are lethal. Read More

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http://doi.wiley.com/10.1002/em.22265
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http://dx.doi.org/10.1002/em.22265DOI Listing
April 2019
16 Reads
2.630 Impact Factor

Bioflavonoids promote stable translocations between MLL-AF9 breakpoint cluster regions independent of normal chromosomal context: Model system to screen environmental risks.

Environ Mol Mutagen 2019 03 2;60(2):154-167. Epub 2018 Nov 2.

Department of Biological Sciences, University of North Carolina at Charlotte, 9201 University City Boulevard, Charlotte, North Carolina.

Infant acute leukemias are aggressive and characterized by rapid onset after birth. The majority harbor translocations involving the MLL gene with AF9 as one of its most common fusion partners. MLL and AF9 loci contain breakpoint cluster regions (bcrs) with sequences hypothesized to be targets of topoisomerase II inhibitors that promote translocation formation. Read More

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http://doi.wiley.com/10.1002/em.22245
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http://dx.doi.org/10.1002/em.22245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6363851PMC
March 2019
9 Reads

Mutagenic Activity of N-Nitrosodiethylamine in Cell Lines Expressing Human CYP2E1-Adequacy of Dimethylsulfoxide as Solvent.

Environ Mol Mutagen 2019 04 29;60(3):214-226. Epub 2018 Nov 29.

Department of Toxicology, School of Public Health, Southern Medical University, Guangzhou, People's Republic of China.

Human CYP2E1 metabolizes many xenobiotics of low-molecular weight, thereby activating various promutagens/procarcinogens. In toxicological studies in vitro, dimethylsulfoxide (DMSO) is a common vehicle for organic compounds. However, it was observed to potently inhibit CYP2E1 activity. Read More

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http://doi.wiley.com/10.1002/em.22264
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http://dx.doi.org/10.1002/em.22264DOI Listing
April 2019
13 Reads

Genetic variation associated with chromosomal aberration frequency: A genome-wide association study.

Environ Mol Mutagen 2019 01 3;60(1):17-28. Epub 2018 Oct 3.

Department of Molecular Genetic Epidemiology, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 580, 69120, Heidelberg, Germany.

Chromosomal aberrations (CAs) in human peripheral blood lymphocytes (PBL) measured with the conventional cytogenetic assay have been used for human biomonitoring of genotoxic exposure for decades. CA frequency in peripheral blood is a marker of cancer susceptibility. Previous studies have shown associations between genetic variants in metabolic pathway, DNA repair and major mitotic checkpoint genes and CAs. Read More

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http://doi.wiley.com/10.1002/em.22236
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http://dx.doi.org/10.1002/em.22236DOI Listing
January 2019
32 Reads
2.630 Impact Factor

In vitro exposure to CPF affects bovine sperm epigenetic gene methylation pattern and the ability of sperm to support fertilization and embryo development.

Environ Mol Mutagen 2019 01 26;60(1):85-95. Epub 2018 Oct 26.

Dipartimento di Biologia, Università di Napoli Federico II, Naples, Italy.

Several studies have demonstrated that overexposure to pesticides can reduce mammalian sperm quality, impairing male fertility. Chlorpyrifos (CPF), a widely used organophosphate pesticide, was shown to impair spermatogenesis by inducing the formation of highly reactive toxic intermediates. To gain further insight into the mechanisms underlying the cytotoxicity and genotoxicity of CPF, bovine spermatozoa were exposed in vitro to environmental CPF concentrations and the motility, in vitro fertilization rates, DNA fragmentation, chromatin alterations, and methylation patterns were assessed. Read More

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http://doi.wiley.com/10.1002/em.22242
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http://dx.doi.org/10.1002/em.22242DOI Listing
January 2019
13 Reads

Evaluation of U. S. National Toxicology Program (NTP) mouse lymphoma assay data using International Workshop on Genotoxicity Tests (IWGT) and the Organization for Economic Co-Operation and Development (OECD) criteria.

Environ Mol Mutagen 2018 12 25;59(9):829-841. Epub 2018 Oct 25.

Ramboll, Little Rock, Arkansas.

The forward gene mutation mouse lymphoma assay (MLA) is widely used, as part of a regulatory test battery, to identify the genotoxic potential of chemicals. It identifies mutagens capable of inducing a variety of genetic events. During the 1980s and early 1990s, the U. Read More

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http://doi.wiley.com/10.1002/em.22250
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http://dx.doi.org/10.1002/em.22250DOI Listing
December 2018
6 Reads

Toxic and Genotoxic Effects of Silver Nanoparticles in Drosophila.

Environ Mol Mutagen 2019 04 29;60(3):277-285. Epub 2018 Nov 29.

Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain.

The in vivo model Drosophila melanogaster was used here to determine the detrimental effects induced by silver nanoparticles (AgNPs) exposure. The main aim was to explore its interaction with the intestinal barrier and the genotoxic effects induced in hemocytes. The observed effects were compared with those obtained by silver nitrate, as an agent acting via the release of silver ions. Read More

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http://doi.wiley.com/10.1002/em.22262
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http://dx.doi.org/10.1002/em.22262DOI Listing
April 2019
11 Reads

In Utero Exposure to Benzo[a]pyrene Induces Ovarian Mutations at Doses That Deplete Ovarian Follicles in Mice.

Environ Mol Mutagen 2018 Oct 24. Epub 2018 Oct 24.

Environmental Health Science and Research Bureau, Health Canada, Ottawa, Ontario, Canada.

Polycyclic aromatic hydrocarbons like benzo[a]pyrene (BaP) are ubiquitous environmental contaminants formed during incomplete combustion of organic materials. Our prior work showed that transplacental exposure to BaP depletes ovarian follicles and increases prevalence of epithelial ovarian tumors later in life. We used the MutaMouse transgenic rodent model to address the hypothesis that ovarian mutations play a role in tumorigenesis caused by prenatal exposure to BaP. Read More

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http://dx.doi.org/10.1002/em.22261DOI Listing
October 2018
1 Read

Pilot studies evaluating the nongenotoxic rodent carcinogens phenobarbital and clofibrate in the rat Pig-a assay.

Environ Mol Mutagen 2019 01 19;60(1):42-46. Epub 2018 Oct 19.

Toxicology and Environmental Research and Consulting, The Dow Chemical Company, Midland, Michigan.

The Pig-a assay is an emerging and promising in vivo method to determine mutagenic potential of chemicals. Since its development in 2008, remarkable progress has been made in harmonizing and characterizing the test procedures, primarily using known mutagenic chemicals. The purpose of the present study was to evaluate specificity of the Pig-a assay using two nongenotoxic and well-characterized rodent liver carcinogens, phenobarbital and clofibrate, in male F344/DuCrl rats. Read More

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http://dx.doi.org/10.1002/em.22232DOI Listing
January 2019
2 Reads

Using a gene expression biomarker to identify DNA damage-inducing agents in microarray profiles.

Environ Mol Mutagen 2018 12 17;59(9):772-784. Epub 2018 Oct 17.

Environmental Health Science and Research Bureau, Health Canada, Ottawa, Ontario, Canada.

High-throughput transcriptomic technologies are increasingly being used to screen environmental chemicals in vitro to provide mechanistic context for regulatory testing. The TGx-DDI biomarker is a 64-gene expression profile generated from testing 28 model chemicals or treatments (13 that cause DNA damage and 15 that do not) in human TK6 cells. While the biomarker is very accurate at predicting DNA damage inducing (DDI) potential using the nearest shrunken centroid method, the broad utility of the biomarker using other computational methods is not fully known. Read More

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http://doi.wiley.com/10.1002/em.22243
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http://dx.doi.org/10.1002/em.22243DOI Listing
December 2018
4 Reads

Intestinal polycyclic aromatic hydrocarbon-DNA adducts in a population of beluga whales with high levels of gastrointestinal cancers.

Environ Mol Mutagen 2019 01 11;60(1):29-41. Epub 2018 Oct 11.

Canadian Wildlife Health Cooperative, Département de pathologie et microbiologie, Faculté de médecine vétérinaire, Université de Montréal, St. Hyacinthe, PQ, J2S2M2, Canada.

Carcinogenic polycyclic aromatic hydrocarbons (PAHs) were disposed directly into the Saguenay River of the St. Lawrence Estuary (SLE) by local aluminum smelters (Quebec, Canada) for 50 years (1926-1976). PAHs in the river sediments are likely etiologically related to gastrointestinal epithelial cancers observed in 7% of 156 mature (>19-year old) adult beluga found dead along the shorelines. Read More

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http://doi.wiley.com/10.1002/em.22251
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http://dx.doi.org/10.1002/em.22251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320268PMC
January 2019
13 Reads

Gamma-H2AX immunofluorescence for the detection of tissue-specific genotoxicity in vivo.

Environ Mol Mutagen 2019 01 11;60(1):4-16. Epub 2018 Oct 11.

Novartis Institutes for BioMedical Research, 4002 Basel, Switzerland.

The phosphorylation of histone H2AX in Serine 139 (gamma-H2AX) marks regions of DNA double strand breaks and contributes to the recruitment of DNA repair factors to the site of DNA damage. Gamma-H2AX is used widely as DNA damage marker in vitro, but its use for genotoxicity assessment in vivo has not been extensively investigated. Here, we developed an image analysis system for the precise quantification of the gamma-H2AX signal, which we used to monitor DNA damage in animals treated with known genotoxicants (EMS, ENU and doxorubicin). Read More

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http://dx.doi.org/10.1002/em.22238DOI Listing
January 2019
12 Reads