27,795 results match your criteria Enteroviruses


Google Trends-based non-English language query data and epidemic diseases: a cross-sectional study of the popular search behaviour in Taiwan.

BMJ Open 2020 Jul 5;10(7):e034156. Epub 2020 Jul 5.

Center for Tropical Medicine and Infectious Disease Research, Kaohsiung Medical University, Kaohsiung, Taiwan

Objective: This study developed a surveillance system suitable for monitoring epidemic outbreaks and assessing public opinion in non-English-speaking countries. We evaluated whether social media reflects social uneasiness and fear during epidemic outbreaks and natural catastrophes.

Design: Cross-sectional study. Read More

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http://dx.doi.org/10.1136/bmjopen-2019-034156DOI Listing

Human antibodies neutralize enterovirus D68 and protect against infection and paralytic disease.

Sci Immunol 2020 Jul;5(49)

Department of Pediatrics (Infectious Diseases), Vanderbilt University Medical Center, Nashville, TN, USA.

Enterovirus D68 (EV-D68) causes outbreaks of respiratory illness, and there is increasing evidence that it causes outbreaks of acute flaccid myelitis (AFM). There are no licensed therapies to prevent or treat EV-D68 infection or AFM disease. We isolated a panel of EV-D68-reactive human monoclonal antibodies that recognize diverse antigenic variants from participants with prior infection. Read More

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http://dx.doi.org/10.1126/sciimmunol.aba4902DOI Listing

Identification of dibucaine derivatives as novel potent enterovirus 2C helicase inhibitors: In vitro, in vivo, and combination therapy study.

Eur J Med Chem 2020 Jun 25;202:112310. Epub 2020 Jun 25.

Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, Frontier Science Center for Immunology and Metabolism, Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China. Electronic address:

Enterovirus A71 (EV-A71) is a human pathogen causing hand, foot and mouth disease (HFMD) which seriously threatened the safety and lives of infants and young children. However, there are no licensed direct antiviral agents to cure the HFMD. In this study, a series of quinoline formamide analogues as effective enterovirus inhibitors were developed, subsequent systematic structure-activity relationship (SAR) studies demonstrated that these quinoline formamide analogues exhibited good potency to treat EV-A71 infection. Read More

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http://dx.doi.org/10.1016/j.ejmech.2020.112310DOI Listing

GABA-B Receptor Encephalitis Triggered by Enterovirus Encephalitis in a Patient With Small Cell Lung Cancer: A Case Report.

Neurologist 2020 Jul;25(4):106-108

Department of Neurology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, South Korea.

Introduction: Encephalitis with gamma-aminobutyric acid (GABA)-B receptor antibodies (GABA-B receptor encephalitis) is known to have underlying neoplastic condition in half of the cases; however, there could be an additional event that could work as a trigger factor. Here, we report a patient with GABA-B receptor encephalitis associated with small cell lung cancer, which was probably triggered by enterovirus encephalitis.

Case Report: A 53-year-old man was admitted for a seizure, following fever and headache for 3 days. Read More

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http://dx.doi.org/10.1097/NRL.0000000000000283DOI Listing

Enterovirus-related rhombencephalitis and myelitis in the third trimester of pregnancy: A case report highlighting clinico-radiological findings at diagnosis and follow-up.

Radiol Case Rep 2020 Aug 23;15(8):1323-1330. Epub 2020 Jun 23.

Dept. of Neuroradiology, National Neuroscience Institute, 11 Jln Tan Tock Seng, 308433, Singapore.

Rhombencephalitis (RE) refers to inflammatory diseases involving the brainstem and cerebellum. Although RE is a rare entity, it is associated with high morbidity and mortality. The management of such patients is often challenging in terms of identifying the etiology and defining prognosis. Read More

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http://dx.doi.org/10.1016/j.radcr.2020.05.062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322137PMC

The epidemiology of admission-requiring pediatric respiratory infections in a Japanese community hospital using multiplex PCR.

Jpn J Infect Dis 2020 Jun 30. Epub 2020 Jun 30.

Department of Pediatrics, Nara Prefecture General Medical Center, Japan.

Respiratory tract infections (RTIs) are the most common diseases globally among children. This study aimed to assess the epidemiology of admission-requiring pediatric RTI cases and to evaluate the effect of pathogen type on length of hospital stay (LOS) using the FilmArray respiratory panel, a multiplex PCR test. The age-specific distribution and seasonality of viruses were investigated between March 26, 2018 and April 12, 2019. Read More

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http://dx.doi.org/10.7883/yoken.JJID.2020.154DOI Listing

Rapid and sensitive direct detection and identification of poliovirus from stool and environmental surveillance samples using nanopore sequencing.

J Clin Microbiol 2020 Jul 1. Epub 2020 Jul 1.

Department of Infectious Disease Epidemiology, Imperial College London, London, W2 1PG, UK.

Global poliovirus surveillance involves virus isolation from stool and environmental samples, intratypic differential (ITD) by PCR and sequencing of the VP1 region to distinguish vaccine (Sabin), vaccine-derived and wild-type polioviruses and ensure an appropriate response. This cell-culture algorithm takes 2-3 weeks on average between sample receipt and sequencing. Direct detection of viral RNA using PCR allows faster detection but has traditionally faced challenges related to poor sensitivity and difficulties in sequencing common samples containing poliovirus and enterovirus mixtures. Read More

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http://dx.doi.org/10.1128/JCM.00920-20DOI Listing

A Mouse Model for Infection with Enterovirus A71 in Small Extracellular Vesicles.

mSphere 2020 Jul 1;5(4). Epub 2020 Jul 1.

Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China

Enterovirus A71 (EV-A71) is the major pathogen of hand, foot, and mouth disease (HFMD); in some severe cases, it could develop into central nervous system (CNS) disease such as aseptic meningitis, encephalitis, and neurogenic pulmonary edema in children under 5 years. The EV-A71 pathogenesis which is involved with the CNS is unclear due to the lack of a simple and reliable mouse model thus far. Most clinical EV-A71 isolates could not effectively infect the neonatal mouse, which used to be an EV-A71 infection model. Read More

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http://dx.doi.org/10.1128/mSphere.00377-20DOI Listing

Experimental immunization of mice with a recombinant bovine enterovirus vaccine expressing BVDV E0 protein elicits a long-lasting serologic response.

Virol J 2020 Jul 1;17(1):88. Epub 2020 Jul 1.

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2, Yuan Ming Yuan West Road Haidian District, Beijing, 100193, China.

Background: Bovine viral diarrhea virus (BVDV) is a cause of substantial economic loss to the cattle industry worldwide, and there are currently no effective treatment or preventive measures. Bovine enterovirus (BEV) has a broad host range with low virulence and is a good candidate as a viral vaccine vector. In this study, we explored new insertion sites for the expression of exogenous genes in BEV, and developed a recombinant infectious cDNA clone for BEV BJ101 strain expressing BVDV E0 protein. Read More

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http://dx.doi.org/10.1186/s12985-020-01338-6DOI Listing

The Potential of Plant-made Vaccines to Fight Picornavirus.

Expert Rev Vaccines 2020 Jul 1. Epub 2020 Jul 1.

Laboratorio de Biofarmacéuticos recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico; Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí , San Luis Potosí, Mexico.

Introduction: Several are pathogens that generate serious problems for human and animal health worldwide. Vaccination is an attractive approach to fight against picornaviruses. In this regard, the development of low-cost vaccines is a priority to ensure coverage; especially in developing and low-income countries. Read More

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http://dx.doi.org/10.1080/14760584.2020.1791090DOI Listing

Outcomes of patients with Severe Acute Respiratory Infections (SARI) admitted to the intensive care unit: results from the Egyptian Surveillance Study 2010-2014.

Multidiscip Respir Med 2020 Jan 9;15(1):465. Epub 2020 Jun 9.

Department of Clinical Pathology, Faculty of Medicine, Mansura University, Mansura, Egypt.

Background: Few data exist about respiratory viral infections in Egyptian patients. Hereby we describe the outcomes of hospitalized Egyptian patients with severe acute respiratory infections (SARI) admitted to the ICU.

Methods: A prospective study in which all hospitalized patients meeting the WHO case definition for SARI and admitted to the ICU, during the period 2010-2014, were enrolled. Read More

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http://dx.doi.org/10.4081/mrm.2020.465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296279PMC
January 2020

Multiplexed High-Throughput Serological Assay for Human Enteroviruses.

Microorganisms 2020 Jun 26;8(6). Epub 2020 Jun 26.

Faculty of Medicine and Health Technology, Tampere University, 33520 Tampere, Finland.

Immunological assays detecting antibodies against enteroviruses typically use a single enterovirus serotype as antigen. This limits the ability of such assays to detect antibodies against different enterovirus types and to detect possible type-specific variation in antibody responses. We set out to develop a multiplexed assay for simultaneous detection of antibodies against multiple enterovirus and rhinovirus types encompassing all human infecting species. Read More

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http://dx.doi.org/10.3390/microorganisms8060963DOI Listing

Intratonsillar Detection of 27 Distinct Viruses: A Cross-Sectional Study.

J Med Virol 2020 Jun 30. Epub 2020 Jun 30.

Department of Pediatrics and Adolescent Medicine, Turku University Hospital and University of Turku, Turku, Finland.

Background: Palatine tonsils have been observed to harbor several distinct respiratory and herpes viruses in separate studies. In this study the presence of these viruses in palatine tonsils was comprehensively studied in both children and adults.

Methods: A cross-sectional analysis of 181 patients (median age 22 years, range 2. Read More

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http://dx.doi.org/10.1002/jmv.26245DOI Listing

Novel phosphatidylinositol 4-kinases III beta (PI4KIIIβ) inhibitors discovered by virtual screening using free energy models.

J Comput Aided Mol Des 2020 Jun 30. Epub 2020 Jun 30.

LASSBio - Laboratório de Avaliação e Síntese de Substâncias Bioativas, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, CCS, Cidade Universitária, Avenida Carlos Chagas Filho 373, Rio de Janeiro, RJ, ZIP 21941-910, Brazil.

Herein, the LASSBio Chemical Library is presented as a valuable source of compounds for screening to identify hits suitable for subsequent hit-to-lead optimization stages. A feature of the LASSBio Chemical Library worth highlighting is the fact that it is a smart library designed by medicinal chemists with pharmacological activity as the main priority. The great majority of the compounds part of this library have shown in vivo activity in animal models, which is an indication that they possess overall favorable bioavailability properties and, hence, adequate pharmacokinetic profiles. Read More

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http://dx.doi.org/10.1007/s10822-020-00327-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324290PMC

Long-Term Outcomes of Pediatric Enterovirus Infection in Taiwan: A Population-Based Cohort Study.

Front Pediatr 2020 12;8:285. Epub 2020 Jun 12.

Children's Medical Center, Taichung Veterans General Hospital, Taichung, Taiwan.

The major burden of diseases in childhood has shifted from infectious diseases to chronic health conditions in recent decades. Although the rates of infectious diseases have decreased, the incidence of chronic diseases stemming from infectious agents continues to grow. Enterovirus is a major infectious disease of childhood and has been linked to numerous chronic diseases. Read More

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http://dx.doi.org/10.3389/fped.2020.00285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303813PMC

Remdesivir (GS-5734) Impedes Enterovirus Replication Through Viral RNA Synthesis Inhibition.

Front Microbiol 2020 12;11:1105. Epub 2020 Jun 12.

Department of Microbiology, School of Preclinical Medicine, Fourth Military Medical University, Xi'an, China.

Human enteroviruses are responsible for diverse diseases, from mild respiratory symptoms to fatal neurological complications. Currently, no registered antivirals have been approved for clinical therapy. Thus, a therapeutic agent for the enterovirus-related disease is urgently needed. Read More

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http://dx.doi.org/10.3389/fmicb.2020.01105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304253PMC

A somatic coliphage threshold approach to improve the management of activated sludge wastewater treatment plant effluents in resource-limited regions.

Appl Environ Microbiol 2020 Jun 26. Epub 2020 Jun 26.

Health Sciences Research Institute (Instituto de Investigaciones en Salud (INISA)), Universidad de Costa Rica, Montes de Oca, Costa Rica.

Effective wastewater management is crucial to ensure the safety of water reuse projects and effluent discharge into surface waters. Multiple studies have demonstrated that municipal wastewater treatment with conventional activated sludge processes is inefficient for the removal of the wide spectrum of viruses in sewage. In this study, a well-accepted statistical approach was used to investigate the relationship between viral indicators and human enteric viruses during wastewater treatment in a resource-limited region. Read More

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http://dx.doi.org/10.1128/AEM.00616-20DOI Listing

Diagnostic accuracy of Vidisca-Ngs In patients with suspected central nervous system infections.

Clin Microbiol Infect 2020 Jun 23. Epub 2020 Jun 23.

Amsterdam UMC, University of Amsterdam, Department of Neurology, Center of Infection and Immunity Amsterdam (CINIMA), Meibergdreef 9, Amsterdam, the Netherlands. Electronic address:

Objectives: Confirming the diagnosis in viral central nervous system (CNS) infections can be difficult with current available diagnostic tools. Virus discovery cDNA-AFLP next generation sequencing (VIDISCA-NGS) is a promising viral metagenomic technique, which enables detection of all viruses in a single assay. We performed a retrospective study on diagnostic accuracy of VIDISCA-NGS in cerebrospinal fluid (CSF) of patients with suspected CNS infections. Read More

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http://dx.doi.org/10.1016/j.cmi.2020.06.012DOI Listing

Stop, think SCORTCH: rethinking the traditional 'TORCH' screen in an era of re-emerging syphilis.

Arch Dis Child 2020 Jun 25. Epub 2020 Jun 25.

Department of Paediatric Infectious Diseases, Imperial College Healthcare NHS Trust, London, UK.

Background: The epidemiology of congenital infections is ever changing, with a recent resurgence in syphilis infection rates seen in the UK. Identification of congenital infection is often delayed; early recognition and management of congenital infections is important. Testing modalities and investigations are often limited, leading to missed diagnostic opportunities. Read More

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http://dx.doi.org/10.1136/archdischild-2020-318841DOI Listing

Non-polio enterovirus infections in children with central nervous system disorders in Shanghai, 2016-2018: Serotypes and clinical characteristics.

J Clin Virol 2020 Jun 17;129:104516. Epub 2020 Jun 17.

Department of Infectious Diseases, Children's Hospital of Fudan University, Shanghai, 201102, China. Electronic address:

Background: Non-polio enrerovirus causes a wide spectrum of neurologic syndromes. The epidemiological and clinical profiles of non-polio enrerovirus-associated central nervous system infections vary by regions and over year.

Objectives: This study aimed to understand the prevalence, serotypes and clinical characteristics of enterovirus-associated aseptic meningitis, encephalitis and meningo-encephalitis in children in Shanghai during 2016-2018. Read More

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http://dx.doi.org/10.1016/j.jcv.2020.104516DOI Listing

Fatal underhanded chronic enterovirus infection associated with anti-CD20 monotherapy for central nervous system demyelinating disease.

Mult Scler 2020 Jun 25:1352458520923978. Epub 2020 Jun 25.

Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), Marseille, France; Laboratoire de microbiologie (Assistance Publique - Hôpitaux de Marseille; IHU Méditerranée Infection) Marseille, France.

We report a fatal case of coxsackievirus B4 chronic infection in a 30-year-old woman with a diagnosis of myelin oligodendrocyte glycoprotein antibody-associated disorder controlled by rituximab monotherapy for 3 years. Initially presenting as self-limited meningitis, the infection remained silent for 8 months before the sudden onset of fulminant myocarditis. Analysis of the complete genome showed that the same virus was responsible for both episodes. Read More

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http://dx.doi.org/10.1177/1352458520923978DOI Listing

Uncovering the underhanded: B-cell depletion therapies and severe viral infections - Commentary on 'Fatal underhanded chronic enterovirus infection associated with anti-CD20 monotherapy for central nervous system demyelinating disease'.

Mult Scler 2020 Jun 25:1352458520933541. Epub 2020 Jun 25.

Department of Neurology, Queen's Medical Centre, Nottingham University Hospitals NHS Trust, Nottingham, UK/Division of Clinical Neuroscience, University of Nottingham, Nottingham, UK.

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http://dx.doi.org/10.1177/1352458520933541DOI Listing

Aquaporin‑4 deletion ameliorates enterovirus 71 infection in mice.

Mol Med Rep 2020 Jun 23. Epub 2020 Jun 23.

Department of Neonatal, Lianshui People's Hospital, Huaian, Jiangsu 223400, P.R. China.

Aquaporin-4 (AQP4) is a major water channel of the central nervous system. The present study was designed to determine whether AQP4 deletion could ameliorate enterovirus (EV) 71 infection‑induced hand, foot and mouth disease (HFMD) by inhibiting inflammation and apoptosis in mice. EV 71 strains were injected into neonatal BALB/c mice to induce HFMD. Read More

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http://dx.doi.org/10.3892/mmr.2020.11265DOI Listing

Antiviral and Immunomodulatory Properties of Antimicrobial Peptides Produced by Human Keratinocytes.

Front Microbiol 2020 3;11:1155. Epub 2020 Jun 3.

Laboratoire de Virologie et Mycobactériologie, CHU de Poitiers, Poitiers, France.

Keratinocytes, the main cells of the epidermis, are the first site of replication as well as the first line of defense against many viruses such as arboviruses, enteroviruses, herpes viruses, human papillomaviruses, or vaccinia virus. During viral replication, these cells can sense virus associated molecular patterns leading to the initiation of an innate immune response composed of pro-inflammatory cytokines, chemokines, and antimicrobial peptides. Human keratinocytes produce and secrete at least nine antimicrobial peptides: human cathelicidin LL-37, types 1-4 human β-defensins, S100 peptides such as psoriasin (S100A7), calprotectin (S100A8/9) and koebnerisin (S100A15), and RNase 7. Read More

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http://dx.doi.org/10.3389/fmicb.2020.01155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283518PMC

Regulation of Apoptosis by Enteroviruses.

Front Microbiol 2020 3;11:1145. Epub 2020 Jun 3.

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

Enterovirus infection can cause a variety of diseases and severely impair the health of humans, animals, poultry, and other organisms. To resist viral infection, host organisms clear infected cells and viruses via apoptosis. However, throughout their long-term competition with host cells, enteroviruses have evolved a series of mechanisms to regulate the balance of apoptosis in order to replicate and proliferate. Read More

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http://dx.doi.org/10.3389/fmicb.2020.01145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283464PMC

Risk Assessment and Virological Monitoring Following an Accidental Exposure to Concentrated Sabin Poliovirus Type 3 in France, November 2018.

Vaccines (Basel) 2020 Jun 22;8(2). Epub 2020 Jun 22.

Centre National de Référence des Entérovirus et Parechovirus, WHO National Poliovirus Laboratory, Institut des Agents Infectieux, Groupement Hospitalier Nord, Hospices Civils de Lyon, 69317 Lyon, France.

The safe and secure containment of infectious poliovirus (PV) in facilities where live PV are handled is the condition to achieve and maintain poliomyelitis eradication. Despite precautions to minimize the risk of release of PV from such facilities to the environment, breaches of containment have already been documented. Here, we report the management of an incident that occurred on 30 November 2018 in a French vaccine manufacturing plant. Read More

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http://dx.doi.org/10.3390/vaccines8020331DOI Listing

Enteric viruses circulating in undiagnosed central nervous system infections at tertiary hospital in São José do Rio Preto, São Paulo, Brazil.

J Med Virol 2020 Jun 24. Epub 2020 Jun 24.

São José do Rio Preto School of Medicine (FAMERP), São José do Rio Preto, São Paulo, Brazil.

Enterovirus (EV) is commonly associated with central nervous system (CNS) syndromes. Recently, gastroenteric viruses, including rotavirus (RVA), human astrovirus (HAstV) and norovirus (NoV), have also been associated with CNS neurological disorders. The aim of the present study was to investigate the presence of EV, RVA, HAstV and NoV associated to CNS infections with undiagnosed etiology in Northwest region of São Paulo State, Brazil, and to conduct the molecular characterization of the positive samples detected. Read More

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http://dx.doi.org/10.1002/jmv.26216DOI Listing

Junctional ectopic tachycardia in neonatal enterovirus myocarditis.

Clin Case Rep 2020 Jun 6;8(6):987-990. Epub 2020 Apr 6.

Department for Pediatric Cardiology University of Leipzig - Heart Center Leipzig Germany.

Differential diagnosis is challenging in poor conditioned neonates referred to the emergency room. Infectious disease is common, yet tachycardia should alert the clinician to look for cardiac arrhythmia and comprise. Tachycardia can lead to cardiomyopathy and should warrant further diagnostics for myocarditis, especially in rare or unusual combination of arrhythmias. Read More

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http://dx.doi.org/10.1002/ccr3.2796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303854PMC

Clinical Characteristics of 5 COVID-19 Cases With Non-respiratory Symptoms as the First Manifestation in Children.

Front Pediatr 2020 12;8:258. Epub 2020 May 12.

Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

An outbreak of the novel coronavirus disease 2019 (COVID-19) occurred in Wuhan, China, in December 2019, which then rapidly spread to more than 80 countries. However, detailed information on the characteristics of COVID-19 in children is still scarce. Five patients with non-respiratory symptoms as the first manifestation were hospitalized from the emergency department, and were later confirmed to have COVID-19, between 23 January and 20 February 2020, at the Wuhan Children's Hospital. Read More

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http://dx.doi.org/10.3389/fped.2020.00258DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235428PMC

[Clinical features of neonatal enterovirus infection].

Zhongguo Dang Dai Er Ke Za Zhi 2020 Jun;22(6):638-642

National Clinical Research Center for Child Health/Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, China.

Objective: To study the clinical features of neonatal enterovirus infection, especially severe enterovirus infection.

Methods: A retrospective analysis was performed for the clinical data of 244 neonates with enterovirus infection. According to the severity of infection, they were divided into a common infection group with 231 neonates and a severe infection group with 13 neonates. Read More

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Detection of enteroviruses in urban sewage by next generation sequencing and its application in environmental surveillance.

Sci Total Environ 2020 Aug 22;728:138818. Epub 2020 Apr 22.

Shandong Center for Disease Control and Prevention, 16992 Jingshi Road, Jinan 250014, China; School of Public Health, Shandong University, 44 Wenhuaxi Road, Jinan 250012, China. Electronic address:

Environmental surveillance has been used successfully in monitoring enterovirus (EV) circulation; however cell culture method may introduce a selective bias in those EV strains that are recovered from the environment. In this study, urban sewage samples were collected monthly in Jinan, China in 2018 and concentrated via membrane adsorption/elution method. A P1 seminested RT-PCR (RT-snPCR) and NGS method was developed, by which amplicons of 4000 nucleotide in length covering the entire P1 region of EVs were obtained from sewage concentrates and were further analyzed by next generation sequencing (NGS). Read More

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http://dx.doi.org/10.1016/j.scitotenv.2020.138818DOI Listing

Characterization of a new anti-enterovirus 68 compound purified from avocado.

ACS Infect Dis 2020 Jun 22. Epub 2020 Jun 22.

One of the major challenges in development of anti-enterovirus (EV) drug is in the safety of the drug. Here, we attempt to identity anti-EV compound from an edible plant extract library, and identify potent anti-enterovirus 68 (EV-D68) activity in avocado (Persea americana). The purified identity is determined as 2,4-(12Z,15Z)-heneicosa-12,15-diene-1,2,4-triol, and named avoenin. Read More

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http://dx.doi.org/10.1021/acsinfecdis.0c00404DOI Listing

Rac1-dependent endocytosis and Rab5-dependent intracellular trafficking are required by Enterovirus A71 and Coxsackievirus A10 to establish infections.

Biochem Biophys Res Commun 2020 Aug 6;529(1):97-103. Epub 2020 Jun 6.

Shanghai Public Health Clinical Center & Institutes of Biomedical Sciences, Fudan University, Shanghai, China. Electronic address:

Enterovirus A71 (EVA71) and Coxsackievirus A10 (CVA10) are representative types of Enterovirus A. Dependent on the host cell types, the EVA71 entry may utilize clathrin-, caveola-, and endophilin-A2-mediated endocytosis. However, the cell-entry and intracellular trafficking pathways of CVA10, using KREMEN1 as its receptor, are unclear. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.058DOI Listing

Development of broad-spectrum enterovirus antivirals based on quinoline scaffold.

Bioorg Chem 2020 Jun 1;101:103981. Epub 2020 Jun 1.

Department of Pharmacology and Toxicology, College of Pharmacy, The University of Arizona, Tucson, AZ 85721, United States. Electronic address:

Non-polio enteroviruses such as enterovirus A71 (EV-A71), EV-D68, and coxsackievirus B3 (CVB3) are significant human pathogens with disease manifestations ranging from mild flu-like symptoms to more severe encephalitis, myocarditis, acute flaccid paralysis/myelitis, and even death. There is currently no effective antivirals to prevent or treat non-polio enterovirus infection. In this study, we report our progress in developing potent and broad-spectrum antivirals against these non-polio enteroviruses. Read More

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http://dx.doi.org/10.1016/j.bioorg.2020.103981DOI Listing

Low function of natural killer cells in treated classic Menkes disease.

Turk J Pediatr 2020 ;62(3):498-500

Division of Pediatric Neurology, University of South Alabama Children's and Women's Hospital, Mobile, Alabama.

Background: Menkes disease (MD) is a rare lethal X-linked, multisystem disorder of copper metabolism resulting from mutations in the ATP7A gene. Features such as Ehlers- Danlos syndrome, trichopoliodystrophy, urologic and skeletal changes have been reported. We present a case of classic MD treated with copper infusions who suffered from persistent natural killer (NK) cell dysfunction. Read More

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http://dx.doi.org/10.24953/turkjped.2020.03.021DOI Listing
January 2020

Vitamin D receptor gene methylation in patients with hand, foot, and mouth disease caused by enterovirus 71.

Arch Virol 2020 Jun 18. Epub 2020 Jun 18.

Department of Infectious Diseases, Xi'an Jiaotong University Second Affiliated Hospital, No. 157 Xiwu Road, Xi'an, 710004, China.

To evaluate the epigenetic regulation of the VDR gene in enterovirus 71 (EV71)-associated severe hand, foot, and mouth disease (HFMD), a total of 116 patients with EV71-HFMD, including 58 with mild EV71-HFMD and 58 with severe EV71-HFMD, as well as 60 healthy controls, were enrolled in this study. Quantitative real-time PCR was used to measure the relative levels of VDR mRNA expression, and the methylation status of the VDR promoter was assessed using a MethylTarget™ assay. The DNA methylation levels of the VDR promoter in children with EV71-associated severe HFMD were lower than those in the healthy controls and in children with mild HFMD (P < 0. Read More

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http://dx.doi.org/10.1007/s00705-020-04701-8DOI Listing

The Function and Mechanism of Enterovirus 71 (EV71) 3C Protease.

Curr Microbiol 2020 Jun 15. Epub 2020 Jun 15.

Department of Microbiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, People's Republic of China.

Enterovirus 71 (EV71) is the main pathogen of the hand, foot, and mouth disease. It was firstly isolated from sputum specimens of infants with central nervous system diseases in California in 1969, and has been repeatedly reported in various parts of the world, especially in the Asia-Pacific region. EV71 3C protein is a 183 amino acid cysteine protease that can cleave most structural and non-structural proteins of EV71. Read More

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http://dx.doi.org/10.1007/s00284-020-02082-4DOI Listing

Enterovirus D68-associated respiratory infection in southern Brazil, 2018 - A population-based laboratory surveillance.

J Clin Virol 2020 Jun 11;129:104503. Epub 2020 Jun 11.

Virology Laboratory, Universidade Federal do Paraná, Curitiba, Brazil.

Enterovirus D68 (EV-D68) strain was confirmed in 36/69-52.2% of enterovirus-positive samples collected through surveillance networks for severe acute respiratory infections (SARI) and influenza-like illness (ILI) in southern Brazil in 2018. This finding settles the sustained circulation of EV-D68 in southern Brazil. Read More

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http://dx.doi.org/10.1016/j.jcv.2020.104503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289115PMC

Genetic diversity of enterovirus G detected in faecal samples of wild boars in Japan: identification of novel genotypes carrying a papain-like cysteine protease sequence.

J Gen Virol 2020 Jun 17. Epub 2020 Jun 17.

Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.

The genetic diversity of enterovirus G (EV-G) was investigated in the wild-boar population in Japan. EV-G-specific reverse transcription PCR demonstrated 30 (37.5 %) positives out of 80 faecal samples. Read More

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http://dx.doi.org/10.1099/jgv.0.001446DOI Listing

Therapeutic targets for enterovirus infections.

Expert Opin Ther Targets 2020 Jun 30:1-13. Epub 2020 Jun 30.

Department of Biological and Environmental Science/Nanoscience Center, University of Jyväskylä , Jyväskylä, Finland.

Introduction: Enteroviruses are among the most common viruses causing a huge number of acute and chronic infections leading to high economic costs. Novel nontoxic antivirals that reduce the virus load in acutely infected individuals and from various surfaces are needed to efficiently combat these viruses.

Areas Covered: This review summarizes the recent findings of compounds and tools targeting the enteroviruses and host cell molecules that are crucial for virus infection. Read More

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http://dx.doi.org/10.1080/14728222.2020.1784141DOI Listing

[Surveillance of acute flaccid paralysis in Ivory Coast between 2007 and 2016: Significance and epidemiological profile of non-poliovirus enteroviruses].

Sante Publique 2019 November December;31(6):837-843

Introduction: Côte d'Ivoire's status as a polio-free country requires high quality surveillance of acute flaccid paralysis. Our study aims to determine the prevalence of non-poliovirus enteroviruses found in the surveillance of Acute Flaccid Paralysis (AFP) in Côte d'Ivoire and to study their distribution according to individual characteristics and associated factors.

Method: We conducted an exhaustive descriptive and analytical cross-sectional retrospective study on 3597 cases of acute flaccid paralysis notified in the context of surveillance of AFP from 2007 to 2016 in Côte d'Ivoire. Read More

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http://dx.doi.org/10.3917/spub.196.0837DOI Listing

DNA microarray analysis of dendritic cells infected by .

J Biol Regul Homeost Agents 2020 Mar-Apr;34(2):577-583

Viral immunology Laboratory, Tianjin Second People's Hospital and Tianjin Institute of Hepatology, Tianjin, China.

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http://dx.doi.org/10.23812/19-409-L-52DOI Listing

Inhibition of Enterovirus 71 by Selenium Nanoparticles Loaded with siRNA through Bax Signaling Pathways.

ACS Omega 2020 Jun 13;5(21):12495-12500. Epub 2020 May 13.

Center Laboratory, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No 318 Renminzhong Road, Yuexiu District, Guangzhou 510120, Guangdong, People's Republic of China.

Enterovirus 71 (EV71) is the principal pathogen leading to severe cases of hand, foot, and mouth disease (HFMD). Specific drugs for EV71 are not discovered currently. Small interfering RNA (siRNA) provides a promising antiviral treatment pathway, but it is difficult to cross cell membranes and is easy to degrade. Read More

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http://dx.doi.org/10.1021/acsomega.0c01382DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271353PMC

Prevalence and Management of Severe Hand, Foot, and Mouth Disease in Xiangyang, China from 2008-2013.

Authors:
Jian Liu Jing Qi

J Med Virol 2020 Jun 16. Epub 2020 Jun 16.

Department of Neurology, Affiliated Shenzhen Baoan Hospital, Southern Medical University.

Therapeutic strategies for severe hand, foot, and mouth disease (HFMD) are currently either inconsequent or deficient in evidence. We retrospectively surveyed HFMD outbreaks in Xiangyang from June 2008 to December 2013. HFMD is staged form I to V according to clinical severity and the case with central nervous system involvement is defined as a severe one. Read More

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http://dx.doi.org/10.1002/jmv.26186DOI Listing
June 2020
2.347 Impact Factor

Impact of 5'terminally deleted Enterovirus-B genome on type I IFN response in human cardiomyocytes.

Virologie (Montrouge) 2020 Apr;24(2):126-128

Laboratoire de Virologie, EA4684, CardioVir, Faculté de médecine, Université de Reims Champagne Ardenne, 51 rue Cognacq Jay, 51100 Reims, France, CHU Robert Debré, Laboratoire de Virologie médicale et moléculaire.

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http://dx.doi.org/10.1684/vir.2020.0836DOI Listing

[Practice and thinking of acute respiratory infection surveillance for the response of emerging respiratory diseases in Shanghai].

Zhonghua Liu Xing Bing Xue Za Zhi 2020 Jun 16;41(0):E073. Epub 2020 Jun 16.

Shanghai Institute of Prevention Medicine, Shanghai 200336, China; School of Public Health,Fudan University,Shanghai 200032. China.

Shanghai Municipal Center for Disease Control and Prevention has implemented an active comprehensive surveillance project of acute respiratory infections in adults in Shanghai, including influenza like illness (ILI) and severe acute respiratory infection (SARI). By testing and identifying a variety of respiratory pathogens, it was found that influenza viruses were the main pathogens in 172 ILI cases in 2019. The positive rates of influenza A (H1N1) pdm09 virus, influenza A (H3N2) virus and influenza B virus Victoria lineage were 30. Read More

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http://dx.doi.org/10.3760/cma.j.cn112338-20200421-00616DOI Listing

Virological investigation of genetic variation of enterovirus type 71 in hand, foot and mouth disease.

Exp Ther Med 2020 Jul 7;20(1):543-549. Epub 2020 May 7.

Department of Laboratory, Kunming Yanan Hospital, Kunming, Yunnan 650051, P.R. China.

The aim of the present study was to analyze the sequence of the VP1 gene in enterovirus 71 (EV71) isolates and to explore their genetic evolution, so as to provide a scientific basis for the clinical prevention and treatment of hand, foot and mouth disease. The fecal samples of 590 patients with suspected hand, foot and mouth disease treated at Yan'an Hospital (Kunming, China) between January 2015 and December 2016 were collected and EV71 nucleic acid was detected by fluorescence PCR. The viral RNA of EV71-positive samples was extracted, the VP1 gene was amplified by PCR and the products were sequenced. Read More

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http://dx.doi.org/10.3892/etm.2020.8728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281934PMC

Double-Membrane Vesicles as Platforms for Viral Replication.

Trends Microbiol 2020 Jun 11. Epub 2020 Jun 11.

Section Electron Microscopy, Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, The Netherlands. Electronic address:

Viruses, as obligate intracellular parasites, exploit cellular pathways and resources in a variety of fascinating ways. A striking example of this is the remodelling of intracellular membranes into specialized structures that support the replication of positive-sense ssRNA (+RNA) viruses infecting eukaryotes. These distinct forms of virus-induced structures include double-membrane vesicles (DMVs), found during viral infections as diverse and notorious as those of coronaviruses, enteroviruses, noroviruses, or hepatitis C virus. Read More

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http://dx.doi.org/10.1016/j.tim.2020.05.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289118PMC

A Severe Case of Enterovirus A71 Acute Flaccid Myelitis With Encephalitis.

J Pediatric Infect Dis Soc 2020 Jun 14. Epub 2020 Jun 14.

Department of Pediatrics (Neurology), Vanderbilt University Medical Center, Nashville, Tennessee, USA.

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http://dx.doi.org/10.1093/jpids/piaa067DOI Listing