The Origin, Dynamic Morphology, and PI4P-Independent Formation of Encephalomyocarditis Virus Replication Organelles.
- C E Melia,
- H M van der Schaar,
- A W M de Jong,
- H R Lyoo,
- E J Snijder,
- A J Koster,
- F J M van Kuppeveld,
- M Bárcena
MBio 2018 Apr 17;9(2). Epub 2018 Apr 17.
Department of Cell and Chemical Biology, Section Electron Microscopy, Leiden University Medical Center, Leiden, The Netherlands
Picornaviruses induce dramatic rearrangements of endomembranes in the cells that they infect to produce dedicated platforms for viral replication. These structures, termed replication organelles (ROs), have been well characterized for the genus of the However, it is unknown whether the diverse RO morphologies associated with enterovirus infection are conserved among other picornaviruses. Here, we use serial electron tomography at different stages of infection to assess the three-dimensional architecture of ROs induced by encephalomyocarditis virus (EMCV), a member of the genus of the family of picornaviruses that is distantly related. Read More