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Pharmaceutics 2022 May 31;14(6). Epub 2022 May 31.

Saint-Petersburg Pasteur Institute, Street Mira, 14, 197101 St. Petersburg, Russia.

Bacterial infections have accompanied humanity throughout its history and became vitally important in the pandemic area. The most pathogenic bacteria are multidrug-resistant strains, which have become widespread due to their natural biological response to the use of antibiotics, including uncontrolled use. The current challenge is finding highly effective antibacterial agents of natural origin, which, however, have low solubility and consequently poor bioavailability. Read More

Pharmaceuticals (Basel) 2022 Jun 7;15(6). Epub 2022 Jun 7.

Department of Chemistry, Faculty of Science, University of Dschang, Dschang P.O. Box 67, Cameroon.

Microbial infections are leading causes of death and morbidity all over the world due to the development of the resistance to antibiotics by certain microorganisms. In this study, the chemical exploration of the ethanol (EtOH) extract of the aerial part of (Dracaenaceae) led to the isolation of one previously unreported chalcone derivative, i.e. Read More

Pathogens 2022 Jun 8;11(6). Epub 2022 Jun 8.

Department of Human Pathology, University of Messina, 98125 Messina, Italy.

Infections caused by bacteria have a major impact on public health-related morbidity and mortality. Despite major advances in the prevention and treatment of bacterial infections, the latter continue to represent a significant economic and social burden worldwide. The WHO compiled a list of six highly virulent multidrug-resistant bacteria named ESKAPE (, , , , , and species) responsible for life-threatening diseases. Read More

Microorganisms 2022 Jun 15;10(6). Epub 2022 Jun 15.

Department of Bacteriology, Microbial Ecology and Parasitology, Chair of Microbiology, Jagiellonian University Medical College, 31-121 Cracow, Poland.

Many difficult-to-treat human infections related to catheters and other indwelling devices are caused by bacteria residing in biofilms. One of the key properties of microorganisms residing in a biofilm is decreased susceptibility towards antimicrobial agents. Therefore, many different approaches have been researched to destroy or inhibit biofilm production by bacteria. Read More

Antibiotics (Basel) 2022 Jun 8;11(6). Epub 2022 Jun 8.

Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hnevotinska 3, 779 00 Olomouc, Czech Republic.

This observational retrospective study aimed to analyze whether/how the spectrum of bacterial pathogens and their resistance to antibiotics changed during the worst part of the COVID-19 pandemic (1 November 2020 to 30 April 2021) among intensive care patients in University Hospital Olomouc, Czech Republic, as compared with the pre-pandemic period (1 November 2018 to 30 April 2019). A total of 789 clinically important bacterial isolates from 189 patients were cultured during the pre-COVID-19 period. The most frequent etiologic agents causing nosocomial infections were strains of (17%), (11%), (10%), coagulase-negative staphylococci (9%), (8%), (6%), (5%), (5%) and (5%). Read More

Antibiotics (Basel) 2022 Jun 3;11(6). Epub 2022 Jun 3.

Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal.

Urinary tract infections (UTIs) are one of the most common infectious diseases at the community level. The continue misuse of antimicrobials is leading to an increase in bacterial resistance, which is a worldwide problem. The objective of this work was to study the incidence and pattern of antimicrobial resistance of the main bacteria responsible for UTI in the community of central and northern Portugal, and establish an appropriate empirical treatment. Read More

Trop Med Infect Dis 2022 Jun 12;7(6). Epub 2022 Jun 12.

Institute of Medical Microbiology and Hygiene, Saarland University, 66421 Homburg, Germany.

Urinary tract infections (UTIs) are among the most common infections in sub-Saharan Africa, but microbiological data to guide treatment decisions are limited. Hence, we investigated the bacterial aetiology and corresponding antimicrobial susceptibility patterns in outpatients with UTIs in Bagamoyo, Tanzania. Urine samples from symptomatic individuals were subjected to microbiological examinations for bacterial species identification using conventional methods and disc diffusion-based resistance testing. Read More

J Clin Microbiol 2022 Jun 23:e0002122. Epub 2022 Jun 23.

bioMérieux, La Balme les Grottes, France.

Fosfomycin is a phosphonic acid derivative active against a wide spectrum of Gram-positive and Gram-negative pathogens. It is used for the treatment of uncomplicated urinary tract infections (uUTI) or severe infections by oral or intravenous (i.v. Read More

J Am Acad Orthop Surg Glob Res Rev 2022 Jun 21;6(6). Epub 2022 Jun 21.

From the Orthopedic Department, Henri Mondor Hospital, Aurillac, France (Deluzarches); the Research & Development Department, I.Ceram, Limoges, France (Poli, and Barriere); and the Infectious Diseases Department, ELSAN Polyclinique de Limoges, Limoges, France (Denes).

To describe the use of a porous alumina ceramic loaded with antibiotics for the reconstruction of bilateral tibial fractures in a patient who presented with bone loss and infection after a motorcycle road injury. A 70-year-old man presented open fractures of his both tibiae (proximal involvement on the right side and diaphyseal on the left side). After initial treatment with multiple débridements and the placement of bilateral external fixators, he had bone loss to both tibiae and had developed infections of both legs with multiple organisms identified (Stenotrophomonas maltophilia, Enterobacter cloacae, and Pseudomonas aeruginosa). Read More

JAC Antimicrob Resist 2022 Jun 16;4(3):dlac059. Epub 2022 Jun 16.

Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Medical faculty and University Hospital of Cologne, Cologne, Germany.

Background: With increasing resistance to common antibiotics the treatment of urinary tract infections has become challenging and alternative therapeutic options are needed. In the present study, we evaluate the activity of three older and less frequently used antibiotics against MDR Enterobacterales.

Methods: Susceptibility of mecillinam, temocillin and nitroxoline was assessed in Enterobacterales isolated from urinary specimens with elevated MICs of third-generation cephalosporins. Read More

Int J Antimicrob Agents 2022 Jun 16:106617. Epub 2022 Jun 16.

Departments of Laboratory Medicine and Internal Medicine, China Medical University Hospital, School of Medicine, China Medical University, Taichung, Taiwan; School of Medicine, China Medical University, Taichung, Taiwan; Ph.D Program for Aging, School of Medicine, China Medical University, Taichung, Taiwan; Departments of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan. Electronic address:

In total, 74,570 potentially naturally inducible chromosomal AmpC-producing (PNIC-AmpC) Enterobacterales isolates included in the Antimicrobial Testing Leadership and Surveillance Programme were obtained worldwide from 2012 to 2019 (22,503 from 2012 to 2014 and 52,067 from 2015 to 2019). One hundred seventeen and 711 isolates obtained in 2012-2014 and 2015-2019, respectively, were carbapenem-resistant Enterobacterales (PNIC-AmpC-CRE). The minimum inhibitory concentrations of ceftazidime-avibactam for these isolates against were determined using the broth microdilution method. Read More

Front Med (Lausanne) 2022 30;9:869822. Epub 2022 May 30.

Department of Internal Medicine, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan City, Taiwan.

Introduction: Bloodstream infections are associated with high mortality rates and contribute substantially to healthcare costs, but a consensus on the prognostic benefits of appropriate empirical antimicrobial therapy (EAT) for bacteraemia is lacking.

Methods: We performed a systematic search of the PubMed, Cochrane Library, and Embase databases through July 2021. Studies comparing the mortality rates of patients receiving appropriate and inappropriate EAT were considered eligible. Read More

AMB Express 2022 Jun 15;12(1):77. Epub 2022 Jun 15.

Department of Microbiology and Immunology, National Research Centre, Dokki, 12622, Cairo, Egypt.

Infectious diseases are the most important cause of death worldwide. Many of these diseases show great resistance to drugs and antibiotics with long-term use. Extracts of some medicinal plants have antimicrobial properties, which can treat and overcome these diseases. Read More

J Orthop Trauma 2022 06;36(6):309-316

Department of Biological Sciences, Dartmouth, Hanover, NH.

Objectives: Describe co-occurrence or clustering of microbial taxa in fracture-related infections to inform further exploration of infection-related interactions among them.

Design: Retrospective review.

Setting: Level 1 trauma center. Read More

Microbiol Spectr 2022 Jun 15:e0096422. Epub 2022 Jun 15.

Institute of Integrative Biology of the Cell (I2BC), CNRS, CEA, Paris-Saclay University, Gif-sur-Yvette, France.

The genus Enterobacter includes species responsible for nosocomial outbreaks in fragile patients, especially in neonatal intensive care units (NICUs). Determining the primary source of infection is critical to outbreak management and patient outcomes. In this investigation, we report the management and control measures implemented during an Enterobacter outbreak of bloodstream infections in premature babies. Read More

Adv Drug Deliv Rev 2022 Jun 4;187:114378. Epub 2022 Jun 4.

Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia; Department of Microbiology, Monash University, Clayton, Victoria, Australia. Electronic address:

Antibiotic therapy has become increasingly ineffective against bacterial infections due to the rise of resistance. In particular, ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) have caused life-threatening infections in humans and represent a major global health threat due to a high degree of antibiotic resistance. To respond to this urgent call, novel strategies are urgently needed, such as bacteriophages (or phages), phage-encoded enzymes, immunomodulators and monoclonal antibodies. Read More

J Glob Antimicrob Resist 2022 Jun 3. Epub 2022 Jun 3.

Department of Microbiology, Faculty of Medicine, University of Colombo, Sri Lanka.

Objective: Although Sri Lanka belongs to a region with a high prevalence of extended-spectrum β-lactamase (ESBL), AmpC β-lactamase and carbapenemase-producing Enterobacteriaceae, data regarding antimicrobial resistance (AMR) is limited. We studied the prevalence and diversity of β-lactamases produced by Enterobacteriaceae urinary pathogens from two hospitals in the Western Province of Sri Lanka.

Methods: ESBL, AmpC β-lactamase and carbapenemase production was detected by phenotypic testing followed by genotyping. Read More

JAC Antimicrob Resist 2022 Jun 30;4(3):dlac050. Epub 2022 May 30.

Oxford University Clinical Research Unit, Patan Academy of Health Sciences, Kathmandu, Nepal.

Objectives: Community-onset bloodstream infections (BSIs) caused by carbapenemase-producing complex (ECC) species are increasing internationally. This observation suggests that ECC are emerging pathogens, requiring for detailed understanding on their genomic epidemiology including transmission dynamics and antimicrobial resistance profiles.

Patients And Methods: We performed WGS on 79 spp. Read More

Microb Drug Resist 2022 Jun 30;28(6):698-709. Epub 2022 May 30.

Department of Laboratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.

Infection caused by carbapenem-resistant (CRE) is a global public health problem. We performed whole-genome sequencing to investigate the molecular epidemiological characteristics of local CRE infections and understand the prevalence of hypervirulent carbapenem-resistant (CRKP). Analysis of multiLocus sequence typing (MLST), antibiotic resistance genes, plasmid replicons, virulence genes, and the genetic environment was also performed. Read More

Vaccines (Basel) 2022 Apr 22;10(5). Epub 2022 Apr 22.

Department of Health and Biological Sciences, Abasyn University, Peshawar 25000, Pakistan.

is involved in multiple hospital-associated infections and is resistant to beta-lactam and tetracycline antibiotics. Due to emerging antibiotics resistance in and lack of licensed vaccine availability, efforts are required to overcome the antibiotics crisis. In the current research study, a multi-epitope-based vaccine against was designed using reverse vaccinology and immunoinformatic approaches. Read More

Microorganisms 2022 May 17;10(5). Epub 2022 May 17.

Federal Research Center for Virology and Microbiology, Branch in Saratov, 410028 Saratov, Russia.

The emergence of multidrug-resistant (MDR) bacterial strains is one of the significant global challenges with regard to bacterial drug-resistance control. organisms belong to the complex (ECC) and are commonly recognized as causative agents for hospital infections. Recently, a few MDR strains associated with infection in piglets, calves, and a fox were reported, highlighting the important role of animals and livestock in the emergence and spread of antimicrobial resistance. Read More

Int J Environ Res Public Health 2022 05 16;19(10). Epub 2022 May 16.

Department of Infectology and Travel Medicine, Faculty of Medicine, Louis Pasteur University Hospital, Pavol Jozef Šafarik University, 041 90 Košice, Slovakia.

Inanimate surfaces are often referred to as nosocomial bacterial reservoirs and represent an important vector in the process of spreading pathogens to patients. Most gram-negative rods can survive on inanimate surfaces for several months. The aim of this study is to determine the prevalence and resistance of gram-negative bacteria isolated from the inanimate surfaces of two selected hospital departments. Read More

Int J Environ Res Public Health 2022 05 11;19(10). Epub 2022 May 11.

CESAM (Centre for Marine and Environmental Studies), University of Aveiro, 3810-193 Aveiro, Portugal.

Carbapenems are antibiotics of pivotal importance in human medicine, the efficacy of which is threatened by the increasing prevalence of carbapenem-resistant (CRE). Urban ponds may be reservoirs of CRE, although this hypothesis has been poorly explored. We assessed the proportion of CRE in urban ponds over a one-year period and retrieved 23 isolates. Read More

Medicine (Baltimore) 2022 May 20;101(20):e29217. Epub 2022 May 20.

Centre for Liver Disease Research, Faculty of Medicine, the University of Queensland, Brisbane, QLD, Australia.

Abstract: Spontaneous bacterial peritonitis (SBP), a common infection in patients with cirrhosis and ascites, is associated with high morbidity and mortality. The aim of this study was to investigate changes in the epidemiology of ascites fluid infections over time in an Australian population, including patient demographics, trends in mortality, length of hospital stay and the nature and antibiotic resistance profile of causative organisms.An observational descriptive population-based epidemiological study of patients with cirrhosis admitted to public hospitals in Queensland during 2008-2017 was performed, linking demographic/clinical and microbiology data. Read More

J Med Microbiol 2022 May;71(5)

Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, Reference Services Division, UK Health Security Agency, London, UK.

Increasing numbers of carbapenemase-producing (CPE), which can be challenging to treat, have been referred to the national reference laboratory in England since the early 2000s. Previous studies on CPE in the UK have focussed on localized outbreaks. We applied whole-genome sequencing (WGS) to isolates referred to the national reference laboratory over 30 months to inform our understanding of CPE epidemiology in England. Read More

Front Cell Infect Microbiol 2022 6;12:879409. Epub 2022 May 6.

Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Mobile colistin resistance () genes represent an emerging threat to public health. Reports on the prevalence, antimicrobial profiles, and clonality of MCR-9-producing complex (ECC) isolates on a national scale in China are limited. We screened 3,373 samples from humans, animals, and the environment and identified eleven MCR-9-positive ECC isolates. Read More

Infect Dis Ther 2022 May 21. Epub 2022 May 21.

Department of Orthopaedic Surgery, University of Occupational and Environmental Health, Kitakyushu, Japan.

Introduction: Fracture-related infections (FRIs) are challenging for orthopedic surgeons, as conventional surgical treatment and systemic antimicrobial therapy cannot completely control local infections. Continuous local antibiotic perfusion (CLAP) is a novel and innovative therapy for bone and soft-tissue infections, and is expected to eradicate biofilms by maintaining a sustained high concentration of antimicrobial agents at the infected site. If CLAP therapy can eradicate infection even in cases with implants while preserving the implants, it would be an ideal and effective treatment for local refractory infections. Read More

Med Trop Sante Int 2021 06 7;1(2). Epub 2021 Apr 7.

Service des maladies infectieuses et tropicales, CHU de Fann, BP 5035, Dakar, Sénégal.

Introduction: Bacteremia is associated with high lethality in HIV-infected patients. The widespread use of cotrimoxazole prophylaxis and misuse of antibiotics promote antibacterial resistance. Only few studies have considered this issue in HIV-infected patients. Read More

Virus Res 2022 Jul 13;316:198800. Epub 2022 May 13.

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 501301, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, China.

Enterobacter cloacae is an opportunistic pathogen that widely distributes in various environments, often causing hospital and community-acquired infections. The limited phage resources and poor genomic data hinder the understanding of the lysis mechanism of Enterobacter cloacae phages. In the present study, we characterized a newly discovered lytic phage PZJ0206 that infects an E. Read More

Infect Genet Evol 2022 Aug 11;102:105301. Epub 2022 May 11.

Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:

Objectives: The Enterobacter cloacae complex is responsible for a variety of infections in hospitalized patients and is resistant to β-lactam antibiotics owing to the expression of AmpC β-lactamase. We report emerging resistance in Enterobacter roggenkampii exposed to ceftriaxone and explore the mechanism underlying mutations responsible for this resistance.

Methods: Three strains were derived from different samples from one patient (blood and liver abscess fluid). Read More