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BMJ Open Ophthalmol 2018 10;3(1):e000173. Epub 2018 Nov 10.

Institute for Transfusion Medicine, University Tissue Bank, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Objective: This validation study investigates the treatment of cornea organ culture medium (Modified Eagle Medium, Biochrom GmbH, Berlin, Germany) with RESEP, a new medical device for antibiotics removal, before microbiological testing with BACTEC blood culture bottles.

Methods And Analysis: 10-100 colony forming units of and were inoculated in 9mL of cornea organ culture medium. In group A, the medium was withdrawn with RESEP and treated for 20 min at room temperature, and then inoculated in BACTEC Plus Aerobic/F/Anaerobic/F blood culture bottles. Read More

Front Cell Infect Microbiol 2018 21;8:410. Epub 2018 Nov 21.

Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, United States.

The SOS response is a conserved response to DNA damage that is found in Gram-negative and Gram-positive bacteria. When DNA damage is sustained and severe, activation of error-prone DNA polymerases can induce a higher mutation rate than is normally observed, which is called the SOS mutator phenotype or hypermutation. We previously showed that zinc blocked the hypermutation response induced by quinolone antibiotics and mitomycin C in and . Read More

FEMS Microbiol Ecol 2018 Dec 3. Epub 2018 Dec 3.

Delft University of technology, Department of Biotechnology, Van der Maasweg 9, 2629 HZ Delft, the Netherlands.

A mechanistic understanding of microbial community establishment and product formation in open fermentative systems can aid the development of bioprocesses utilising organic waste. Kinetically, a single rate-limiting substrate is expected to result in one dominant species. Four enrichment cultures were operated to ferment either xylose or glucose in a sequencing batch reactor (SBR) or a continuous-flow stirred tank reactor (CSTR) mode. Read More

World J Microbiol Biotechnol 2018 Nov 30;34(12):186. Epub 2018 Nov 30.

Embrapa Semiárido, BR 428, km 152, s/n, Petrolina, PE, Brazil.

Sorghum [Sorghum bicolor (L.) Moench] is a multipurpose grass cultivated in drylands due to its adaptation to drought. However the characteristics of sorghum-associated bacteria are not known in the Brazilian drylands. Read More

Zhonghua Er Ke Za Zhi 2018 Dec;56(12):907-914

Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China.

To investigate the prevalence and resistance changes of carbapenem-resistant (CRE) strains isolated from children patients of Chinese Bacterial Resistance Surveillance Network (CHINET) from 2005 to 2017. Antimicrobial susceptibility testing was carried out by disk diffusion method (KB method) and automated systems. Results were analyzed according to the Clinical and Laboratory Standards Institute (CLSI) 2017 edition standards. Read More

World Neurosurg 2018 Nov 27. Epub 2018 Nov 27.

Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, USA.

Background: Gram-negative surgical site infections (SSI) following spine surgery are becoming increasingly more common due to a broad perioperative antibiotics usage targeting gram-positive organisms. Enterobacter species have been reported to be the most common gram-negative bacteria following spine surgery.

Methods: We queried our institutional database for Enterobacter SSIs after spine surgery at a single institution from 2009 to 2016. Read More

Adv Clin Exp Med 2018 Nov 30. Epub 2018 Nov 30.

Department of Bacteriology, National Institute of Public Health-National Institute of Hygiene, Warszawa, Poland.

Background: Plasmid-mediated extended-spectrum β-lactamases (ESBLs), 16S rRNA methylases and quinolone resistance mechanisms (PMQRs) are well-known agents conferring resistance to more than 1 antimicrobial in its group. The accumulation of these agents poses, therefore, a serious risk to public health.

Objectives: The objective of this study was to investigate the presence of common ß-lactamases and 16S rRNA methylases in Qnr-producing Enterobacteriaceae and their genetic relatedness. Read More

Lett Appl Microbiol 2018 Nov 29. Epub 2018 Nov 29.

College of Life Sciences, Agricultural University of Hebei, Baoding, 071001, China.

Medicago sativa L. (alfalfa) is an important forage crop throughout the world. Despite the abiotic nutritional components of alfalfa having been extensively studied, there is only limited information on alfalfa endophytes. Read More

Zhonghua Shao Shang Za Zhi 2018 Nov;34(11):802-808

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, the First Affiliated Hospital of Army Medical University (the Third Military Medical University), Chongqing 400038, China.

To analyze the distribution and drug resistance of pathogens from the wounds of thermal burn patients, so as to provide reliable basis for the rational use of antibiotics and the effective control over nosocomial infection. Wound samples of 1 310 thermal burn patients admitted into our burn wards from January 2012 to December 2017 were collected and retrospectively analyzed. API bacteria identification panels and automatical bacteria identification equipment were used to identify pathogens. Read More

Environ Int 2018 Nov 24;123:1-9. Epub 2018 Nov 24.

Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China. Electronic address:

In nature, microbes use extracellular electron transfer (EET) to recover noble metals. Most attention has been paid to the biorecovery process occurring intracellularly and on the cell surface. In this work, we report that Pd nanorods could be biosynthesized by Enterobacter cloacae SgZ-5T in the extracellular space. Read More

Nat Commun 2018 Nov 27;9(1):5010. Epub 2018 Nov 27.

Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, 77030, TX, USA.

Neonatal rotavirus infections are predominantly asymptomatic. While an association with gastrointestinal symptoms has been described in some settings, factors influencing differences in clinical presentation are not well understood. Using multidisciplinary approaches, we show that a complex interplay between human milk oligosaccharides (HMOs), milk microbiome, and infant gut microbiome impacts neonatal rotavirus infections. Read More

J Bacteriol 2018 Nov 26. Epub 2018 Nov 26.

Department of Chemistry, State University of New York, College of Environmental Science and Forestry, Syracuse, New York, USA

Dimethylsulfide (DMS) is a volatile sulfur compound produced mainly from the degradation of dimethylsulfoniopropionate (DMSP) in marine environments. DMS undergoes oxidation to form dimethylsulfoxide (DMSO), dimethylsulfone (DMSO) and methanesulfonate (MSA), all of which occur in terrestrial environments and are accessible for consumption by various microorganisms. The purpose of the current study was to determine how the enhancer-binding proteins SfnR1 and SfnR2 contribute to the utilization of DMS and its derivatives in PAO1. Read More

J Infect Public Health 2018 Nov 23. Epub 2018 Nov 23.

Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia. Electronic address:

Introduction: Nanoparticles (NPs) have become very important owing to their various uses. In this research, an environmentally friendly biological technique was used to synthesize silver nanoparticles with Coriandrum sativum L. The objective of this research to use the source for the fabrication of silver NPs from C. Read More

High Throughput 2018 Nov 23;7(4). Epub 2018 Nov 23.

Laboratorio de Medicina Genómica, Hospital Regional "Primero de Octubre", ISSSTE, Av. Instituto Politécnico Nacional 1669, Lindavista, Gustavo A. Madero, Ciudad de Mexico 07300, Mexico.

Beta-lactam resistant bacteria, which are commonly resident in tertiary hospitals, have emerged as a worldwide health problem because of ready-to-eat vegetable intake. We aimed to characterize the genes that provide resistance to beta-lactam antibiotics in Enterobacteriaceae, isolated from five commercial salad brands for human consumption in Mexico City. In total, twenty-five samples were collected, grown in blood agar plates, and the bacteria were biochemistry identified and antimicrobial susceptibility testing was done. Read More

FEMS Microbiol Lett 2018 Nov 26. Epub 2018 Nov 26.

Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece.

2,3-Butanediol (BDO) is an important platform chemical with wide range of applications in various industries. In the present study, a newly isolated wild Enterobacter sp. strain (FMCC-208) was evaluated towards its ability to produce BDO on media composed of sugars derived from sucrose refinery plant. Read More

Case Rep Nephrol 2018 24;2018:9074245. Epub 2018 Oct 24.

Pediatric Nephrology Center of Excellence, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.

Neonatal jaundice is considered one of the most common reasons for admission to the pediatric medical ward. We report a case of a 1-month-old infant who presented with jaundice but no fever or any other signs of systemic illnesses. Laboratory test results revealed high direct hyperbilirubinemia, and urine culture showed a urinary tract infection with as the causative agent. Read More

Pak J Pharm Sci 2018 Nov;31(6):2341-2346

Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

Present research work was aimed to investigate the biological activities i.e. antibacterial, antifungal, antioxidant, cytotoxic and antitumor activities of crude methanolic extract of Anagallis arvensis L. Read More

Int J Antimicrob Agents 2018 Nov 22. Epub 2018 Nov 22.

Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Laboratory affiliated to Istituto Pasteur Italia - Fondazione Cenci Bolognetti, Rome, Italy. Electronic address:

The rise in antibacterial resistance jeopardizes current therapeutic strategies to control infections, soliciting the development of novel antibacterial drugs with new mechanisms of action. In this work we report the discovery of a potent and selective anti-staphylococcal activity of 3-bromopyruvate (3BP), an antimetabolite in preclinical development phase as an anticancer drug. 3BP showed bactericidal activity against Staphylococcus aureus, with active concentrations comparable to those reported to be effective against cancer cells. Read More

Int J Antimicrob Agents 2018 Nov 22. Epub 2018 Nov 22.

Department of Microbiology, Universitary Hospital 12 de Octubre, Madrid, Spain.; Research Institute Hospital 12 de Octubre (i+12), Universitary Hospital 12 de Octubre, Madrid, Spain.

BMC Microbiol 2018 Nov 23;18(1):175. Epub 2018 Nov 23.

Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, M/S 89-2 4800 Oak Grove Dr, Pasadena, CA, 91109, USA.

Background: The antimicrobial resistance (AMR) phenotypic properties, multiple drug resistance (MDR) gene profiles, and genes related to potential virulence and pathogenic properties of five Enterobacter bugandensis strains isolated from the International Space Station (ISS) were carried out and compared with genomes of three clinical strains. Whole genome sequences of ISS strains were characterized using the hybrid de novo assembly of Nanopore and Illumina reads. In addition to traditional microbial taxonomic approaches, multilocus sequence typing (MLST) analysis was performed to classify the phylogenetic lineage. Read More

Molecules 2018 Nov 20;23(11). Epub 2018 Nov 20.

Department of Process Engineering and Chemical Technology, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland.

The need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products. Hence, in this work a two-step approach for the pre-treatment of energy poplar, including alkaline pre-treatment and enzymatic saccharification followed by fermentation has been studied. Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification. Read More

Arch Argent Pediatr 2018 Dec;116(6):e744-e748

Servicio de Infectología, Hospital de Niños Sor María Ludovica.

Chronic granulomatous disease is an uncommon primary immunodeficiency due to a defect of the killing activity of phagocytes, caused by mutations in any of the genes encoding subunits of the superoxide-generating phagocyte NADPH oxidase system. The incidence is 1 in 250 000 live births. It can occur from infancy to adulthood, usually in children under 2 years. Read More

Biotechnol Biofuels 2018 8;11:305. Epub 2018 Nov 8.

1CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No.189 Songling Road, Laoshan District, Qingdao, 266101 China.

Background: 2-Phenylethanol (2-PE) is a higher aromatic alcohol that is widely used in the perfumery, cosmetics, and food industries and is also a potentially valuable next-generation biofuel. In our previous study, a new strain sp. CGMCC 5087 was isolated to produce 2-PE from glucose through the phenylpyruvate pathway. Read More

Microbiol Res 2019 Jan 6;218:76-86. Epub 2018 Oct 6.

Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico. Electronic address:

Legumes establish symbiotic relationships with different microorganisms, which could function as plant growth promotion microorganisms (PGPM). The finding of new PGPM strains is important to increase plant production avoiding or diminishing the use of industrial fertilizers. Thus, in this work we evaluated the plant growth promotion traits of ten strains isolated from Mimosa pudica root nodules. Read More

F1000Res 2018 1;7:521. Epub 2018 May 1.

J Craig Venter Institute, Rockville, MD, 20850, USA.

The predominant species in clinical isolates is . Many articles, clinicians, and GenBank submissions misname these strains as . The lack of sequenced type strains or named species/subspecies for some clades in the complex complicate the issue. Read More

J Glob Antimicrob Resist 2018 Nov 15;15:290-291. Epub 2018 Nov 15.

Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address:

Objectives: The widespread dissemination of extended-spectrum β-lactamase-producing Enterobacteriaceae has become a major issue in veterinary medicine. However, until now, there has been no report of bacteria with such a phenotype in infected snakes. The aim of this study was to report the first draft genome sequence of an Enterobacter cloacae isolate (SERP1) recovered from a snake with infectious stomatitis. Read More

Acta Biochim Pol 2018 11 17. Epub 2018 Nov 17.

Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy.

The major acid-soluble protein components of the mussel Mytilus galloprovincialis sperm chromatin consist of the protamine-like proteins PL-II, PL-III and PL-IV, an intermediate group of sperm nuclear basic proteins between histones and protamines. The aim of this study was to investigate the bactericidal activity of these proteins since, to date, there are reports on bactericidal activity of protamines and histones, but not on protamine-like proteins. We tested the bactericidal activity of these proteins against Gram-positive bacteria: Enterococcus faecalis and two different strains of Staphylococcus aureus, as well as Gram-negative bacteria: Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella typhmurium, Enterobacter aerogenes, Enterobacter cloacae, and Escherichia coli. Read More

J Antimicrob Chemother 2018 Nov 14. Epub 2018 Nov 14.

Ecotoxicol Environ Saf 2018 Nov 13;169:161-168. Epub 2018 Nov 13.

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China; National Engineering Laboratory for VOCs Pollution Control Material & Technology, University of Chinese Academy of Sciences, Beijing 101408, PR China. Electronic address:

The characteristics of bioaerosol emissions from wastewater treatment plants (WWTPs) have attracted extensive attention. The anaerobic-anoxic-oxic (AO) process, which uses the activated sludge approach to wastewater treatment, is the most widely used process in WWTPs. Concentration, size distribution, population, and exposure risk from bacteria and fungi in bioaerosols of WWTPs using the AO process were studied in this work. Read More

G Chir 2018 Sep-Oct;34(5):284-290

Aim: Few series of osteomyelitis due to multi-drug (MDR) or extensively-drug resistant (XDR) gram-negative bacteria exist. A retrospective study of MDR and XDR gram-negative osteomyelitis cases was performed, aiming to investigate causative organisms, proper surgical and medical management, as well as outcome.

Patients And Methods: All patients, treated at the University hospital of Crete between 2007 and 2016 for acute osteomyelitis, due to MDR or XDR gram-negative pathogens were evaluated. Read More

J Interdiscip Nanomed 2018 Sep 4;3(3):111-121. Epub 2018 Sep 4.

Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Sciences Queen's University Belfast Belfast UK.

One of the most important health concerns in society is the development of nosocomial infections caused by multidrug-resistant pathogens. The purpose of this review is to discuss the issues in current antibiotic therapies and the ongoing progress of developing new strategies for the treatment of ESKAPE pathogen infections, which is acronymized for , Staphylococcus aureus, Klebsiella pneumoniae, , , and species. We not only examine the current issues caused by multidrug resistance but we also examine the barrier effects such as biofilm and intracellular localization exploited by these pathogens to avoid antibiotic exposure. Read More

PLoS One 2018 15;13(11):e0207403. Epub 2018 Nov 15.

Department of Biological Sciences, Masinde Muliro University of Science and Technology, Kakamega, Kenya.

Biological nitrogen fixation (BNF) in legumes plays a critical role in improving soil fertility. Despite this vital role, there is limited information on the genetic diversity and BNF of bacteria nodulating common bean (Phaseolus vulgaris L.). Read More

Bioresour Technol 2018 Nov 2;273:144-152. Epub 2018 Nov 2.

School of Life Sciences, Jilin University, Changchun, Jilin 130012, PR China; National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin 130012, PR China; Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Jilin 130012, PR China. Electronic address:

The objective of this study was to develop a new type of feedstuff by utilizing probiotics to transform agricultural byproducts to meet the livestock feed shortage. In this study, peanut hull powder (PHP) was used as the fermentation substrate, which was inoculated with 18 kinds of feed probiotics combined with two kinds of nutritional additives. The desired value formula was used to select the strains suitable for fermentation of PHP, and the strains were combined as multi-culture starters. Read More

PLoS One 2018 14;13(11):e0207280. Epub 2018 Nov 14.

Floral and Nursery Plants Research Unit, U. S. National Arboretum, U. S. Dept. of Agriculture-Agricultural Research Service, Beltsville, MD, United States of America.

The cool virulent Ralstonia solanacearum race 3 biovar 2 (r3b2) strains cause destructive brown rot of potato. They are quarantined pathogens in Europe and Canada and select agent pathogens in the United States. We previously identified r3b2 (sequevars 1 and 2)-unique fragments that clustered into 32 regions in the genome of R. Read More

Biomed Res Int 2018 22;2018:2723157. Epub 2018 Oct 22.

Department of Microbiology, Federal University of Viçosa, 36570-000 Viçosa, MG, Brazil.

The 16S rDNA of six psychrotrophic Enterobacteriaceae isolated from cold raw milk were sequenced and the isolate 039 was identified as sp., isolates 059, 068, and 071 were identified as , 067 was identified as sp., and 099 was identified as . Read More

Infect Drug Resist 2018 17;11:1881-1891. Epub 2018 Oct 17.

Departments of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan,

Objectives: This study investigated the prevalence rates of carbapenemase positivity, antibiotic susceptibility, and independent predictors of carbapenemase producers among the Enterobacteriaceae isolates recovered from patients with intra-abdominal infections (IAI) in the Asia-Pacific region between 2008 and 2014.

Materials And Methods: Multiplex PCR was used for the detection of specific β-lactamases, while the broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of antibiotics among the IAI-related Enterobacteriaceae isolates. We studied the abovementioned parameters in 484 ertapenem-non-susceptible (Erta-NS) isolates and explored the independent predictors of carbapenemase-producing Enterobacteriaceae (CPE) isolates. Read More

Euro Surveill 2018 Nov;23(45)

Clinical Microbiology and Virology Unit, Careggi University Hospital, Florence, Italy.

BackgroundThe gene is a transferable resistance determinant against colistin, a last-resort antimicrobial for infections caused by multi-resistant Gram-negatives.AimTo study carriage of antibiotic-resistant bacteria in healthy school children as part of a helminth control and antimicrobial resistance survey in the Bolivian Chaco region.MethodsFrom September to October 2016 we collected faecal samples from healthy children in eight rural villages. Read More

Clin Infect Dis 2018 Nov;67(suppl_2):S196-S205

Department of Clinical Laboratory, Peking University People's Hospital, Beijing.

Background: Carbapenem-resistant Enterobacteriaceae (CRE) strains are a major threat to global health. The development of effective control measures requires more detailed phenotypic and genotypic characterization of CRE.

Methods: CRE isolates were collected from 65 hospitals in 25 provinces across China between January 1, 2012, and December 31, 2016. Read More

Cornea 2018 Nov 12. Epub 2018 Nov 12.

Ophthalmic Research Center, Department of Ophthalmology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Purpose: To investigate the rate and agents of contamination in bandage soft contact lenses fitted for management of persistent corneal epithelial defects.

Methods: This prospective comparative case series enrolled 57 consecutive eyes fitted with bandage contact lenses for treatment of persistent corneal epithelial defects. The lenses were collected at the time of epithelial closure or when it was necessary to exchange contact lenses and were immediately placed in sterile tubes containing an enriched thioglycolate liquid medium. Read More

Environ Monit Assess 2018 Nov 12;190(12):716. Epub 2018 Nov 12.

Institute of Soil and Environmental Sciences, University of Agriculture Faisalabad, Faisalabad, 38040, Pakistan.

Microorganisms have great potential to control environmental pollution, particularly industrial sources of water pollution. Currently, leather industry is regarded as the most polluting and suffering from negative impacts due to the pollution it adds to the environment. Chromium, one of the hazardous pollutants discharged from tanneries, is highly toxic and carcinogenic in nature. Read More

Pak J Biol Sci 2018 Jan;21(8):369-375

Background And Objectives: Herbal medicines are getting more importance in the treatment of infections ailments because most of the synthetic drugs have side effects. A large proportion of the developing countries populations depend on herbal remedy for their physical and psychological health needs depend. Hence, the anti-bacterial activity of the mixture of the extracts of Solenostemon monostachyus and Vernonia amygdalina, common medicinal plants use in Africa, Asia and Europe on some Gram-negative bacteria was investigated. Read More

Am J Transl Res 2018 15;10(10):3171-3185. Epub 2018 Oct 15.

Department of Thoracic Surgery, The Second Hospital of Shandong University Shandong, P. R. China.

Emerging evidence suggests the microbiome may affect a number of diseases, including lung cancer. However, the direct relationship between gut bacteria and lung cancer remains uncharacterized. In this study, we directly sequenced the hypervariable V1-V2 regions of the 16S rRNA gene in fecal samples from patients with lung cancer and healthy volunteers. Read More

Mikrochim Acta 2018 Nov 10;185(12):542. Epub 2018 Nov 10.

Department of Chemistry, Shanghai Stomatological Hospital, Fudan University, Shanghai, 200000, China.

A method is described for fast identification of bacteria by combining (a) the enrichment of bacterial cells by using magnetite (FeO) magnetic beads modified with human IgG (IgG@FeO) and (b) MALDI-TOF MS analysis. IgG has affinity to protein A, protein G, protein L and glycans on the surface of bacterial cells, and IgG@FeO. It therefore is applicable to the preconcentration of a range of bacterial species. Read More

Int J Antimicrob Agents 2018 Nov 8. Epub 2018 Nov 8.

Texas Tech University Health Sciences Center, Department of Pharmacy Practice, 5920 Forest Park Rd, Suite 400, Dallas, TX 75235, USA; VA North Texas Health Care System, 4500 S Lancaster, Dallas, TX 75216, USA; University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 7539, USA. Electronic address:

Piperacillin/tazobactam has been associated with nephrotoxicity in patients receiving vancomycin. Its impact on nephrotoxicity in patients with gram-negative bacteremia (GNB) is unclear. This study aims to evaluate piperacillin/tazobactam's impact on nephrotoxicity in patients with GNB. Read More

Microbiologyopen 2018 Nov 9:e737. Epub 2018 Nov 9.

Gastro intestinal Microbiology and Biotechnology, Agricultural Research Council- Animal Production, Irene, South Africa.

Management and disposal of pig farm seepage constitute a serious environmental challenge, and seepage discharge from agricultural waste-water is considered to be one of the greatest contributors of organic substances, bacterial pathogens, and antibiotic resistance genes into the environment. The objectives of this study were to assess the level of bacteriological pollution and to identify the resident antibiotic-resistant genes of culturable bacteria from a studied pig farm seepage. Enumeration of the viable bacterial cell of plated bacteria suspensions (10 to 10  cfu/mL) was performed; also, identification of pure bacterial colonies was done using an API 20E bacterial identification kit. Read More

J Antimicrob Chemother 2018 Nov 8. Epub 2018 Nov 8.

Laboratory of Bacteriology, University Hospital of Dijon, Plateau technique de Biologie, BP 37013, Dijon cedex, France.

Objectives: To characterize the structure of a variant of Acinetobacter genomic island 1 (AGI1) in the Enterobacter cloacae clinical isolate EclC2185 responsible for an 8 year outbreak in Dijon University Hospital.

Methods: The genome was sequenced (Miseq) and de novo assembled (Velvet). PCR and Sanger sequencing were performed to determine the sequence of the genomic island. Read More

Beilstein J Org Chem 2018 17;14:2651-2664. Epub 2018 Oct 17.

Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706, USA.

Quorum sensing (QS) allows many common bacterial pathogens to coordinate group behaviors such as virulence factor production, host colonization, and biofilm formation at high population densities. This cell-cell signaling process is regulated by acyl L-homoserine lactone (AHL) signals, or autoinducers, and LuxR-type receptors in Gramnegative bacteria. SdiA is an orphan LuxR-type receptor found in genera that responds to AHL signals produced by other species and regulates genes involved in several aspects of host colonization. Read More

Microbiome 2018 Nov 8;6(1):201. Epub 2018 Nov 8.

J. Craig Venter Institute, 4120 Capricorn Lane, La Jolla, CA, 92037, USA.

Background: Travelers' diarrhea (TD) is often caused by enterotoxigenic Escherichia coli, enteroaggregative E. coli, other bacterial pathogens, Norovirus, and occasionally parasites. Nevertheless, standard diagnostic methods fail to identify pathogens in more than 40% of TD patients. Read More

Crit Care Med 2018 Nov 7. Epub 2018 Nov 7.

UMR 1137, IAME, Université Paris Diderot, Paris, France.

Objectives: To investigate the respective impact of ventilator-associated pneumonia and ICU-hospital-acquired pneumonia on the 30-day mortality of ICU patients.

Design: Longitudinal prospective studies.

Setting: French ICUs. Read More

Clin Infect Dis 2018 Nov 8. Epub 2018 Nov 8.

Center for Disease Dynamics, Economics & Policy, Washington, DC, USA.

Background: The threat posed by antibiotic resistance is of increasing concern in low- and middle-income countries (LMICs) as their rates of antibiotic use increase. However, an understanding of the burden of resistance is lacking in LMICs, particularly for multi-drug resistant (MDR) pathogens.

Methods: We conducted a retrospective, ten hospital study of the relationship between MDR pathogens and mortality in India. Read More