6,865 results match your criteria Embryonal Carcinoma

Utility of frozen section in pediatric and adolescent malignant ovarian nonseminomatous germ cell tumors: A report from the children's oncology group.

Gynecol Oncol 2022 Jun 21. Epub 2022 Jun 21.

Dana-Farber/Boston Children's Cancer and Blood Disorders Center, United States of America.

Purpose: In adult women, most malignant ovarian tumors are epithelial in origin. The use of intra-operative frozen section to distinguish between benign and malignant histology is reliable in guiding operative decision-making to determine the extent of surgical staging required. Pediatric and adolescent patients with ovarian masses have a much different spectrum of pathology with most tumors arising from germ cell precursors. Read More

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Analyzing the Interaction of RBPJ with Mitotic Chromatin and Its Impact on Transcription Reactivation upon Mitotic Exit.

Methods Mol Biol 2022 ;2472:95-108

The Program in Cellular and Molecular Oncology, University of New Mexico Comprehensive Cancer Center, Albuquerque, NM, USA.

The sequence-specific transcription factor RBPJ, also known as CSL (CBF1, Su(H), Lag1), is an evolutionarily conserved protein that mediates Notch signaling to guide cell fates. When cells enter mitosis, DNA is condensed and most transcription factors dissociate from chromatin; however, a few, select transcription factors, termed bookmarking factors, remain associated. These mitotic chromatin-bound factors are believed to play important roles in maintaining cell fates through cell division. Read More

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Role of the Ubiquitin Ligase RNF149 in the Development of Rat Neonatal Gonocytes.

Front Endocrinol (Lausanne) 2022 13;13:896507. Epub 2022 May 13.

The Research Institute of the McGill University Health Centre, McGill University, Montreal, QC, Canada.

Male reproductive function depends on the formation of spermatogonial stem cells from their neonatal precursors, the gonocytes. Previously, we identified several UPS enzymes dynamically altered during gonocyte differentiation. The present work focuses on understanding the role of the RING finger protein 149 (RNF149), an E3 ligase that we found to be strongly expressed in gonocytes and downregulated in spermatogonia. Read More

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The Design and Synthesis of a New Series of 1,2,3-Triazole-Cored Structures Tethering Aryl Urea and Their Highly Selective Cytotoxicity toward HepG2.

Pharmaceuticals (Basel) 2022 Apr 20;15(5). Epub 2022 Apr 20.

Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand.

Target cancer drug therapy is an alternative treatment for advanced hepatocellular carcinoma (HCC) patients. However, the treatment using approved targeted drugs has encountered a number of limitations, including the poor pharmacological properties of drugs, therapy efficiency, adverse effects, and drug resistance. As a consequence, the discovery and development of anti-HCC drug structures are therefore still in high demand. Read More

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Fluorescent in-situ hybridization test performed on cell blocks is useful for establishing germ cell origin of metastatic tumors with unusual clinical presentation.

Diagn Cytopathol 2022 May 25. Epub 2022 May 25.

Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA.

The majority of germ cell tumors (GCTs) are characterized by iso-chromosome 12p (i12p) abnormality. The aim of this study is to review the cytomorphologic features and analyze the utility of i12p fluorescent in-situ hybridization (FISH) test in diagnosing metastatic GCTs primarily evaluated by cytologic techniques in patients without prior history of GCTs. The laboratory information system was queried over a period of 10 years to search for cases where i12p FISH test was requested on cytology material. Read More

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Expression of Zeb1 in the differentiation of mouse embryonic stem cell.

Open Life Sci 2022 9;17(1):455-462. Epub 2022 May 9.

Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, 1120 Lianhua Road, Shenzhen, Guangdong 518036, China.

Embryonic stem cells (ESCs) differentiation is a process of replication and refinement, and the directional lineage differentiation of ESCs involves the epithelial-mesenchymal transition (EMT)- mesenchymal-epithelial transition (MET) process. A previous study revealed that Zinc finger E-box-binding homeobox 1 (Zeb1) plays a vital role in EMT, which could repress E-cadherin promoter and induce an EMT in cells. To verify the expression of Zeb1 and its correlation with Lin28a in mouse ESCs differentiation, we performed qRT-PCR and western blots to detect the expression of Lin28a mRNA and protein after Zeb1 knockdown. Read More

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Reduced CDH16 expression is linked to poor prognosis in clear cell renal cell carcinoma 16.

Urol Oncol 2022 07 21;40(7):348.e1-348.e8. Epub 2022 May 21.

Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Objective: Cadherin-16 (CDH16) is a member of the cadherin superfamily which is also termed kidney specific cadherin (ksp-cadherin) because of its preferred expression in the normal kidney where it plays a pivotal role in tubulus formation during embryonal development. However, little is known about the prognostic role of CDH16 in renal cell carcinomas.

Methods And Material: CDH16 protein was analyzed by immunohistochemistry in more than 1,300 renal cell carcinomas (RCCs) in a tissue microarray format. Read More

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Decoding the dynamic H3K9cr landscapes during neural commitment of P19 embryonal carcinoma cells.

Biochem Biophys Res Commun 2022 Jul 13;613:187-192. Epub 2022 May 13.

School of Life Sciences and Medicine, Shandong University of Technology, Zibo, Shandong, China.

Histone lysine crotonylation (Kcr) is a novel hydrophobic histone acylation modification, and we recently report its crucial roles in neural differentiation. However, it is still unclear how histone Kcr involve in early neural commitment. Here, we systematically investigate the H3K9cr landscapes during neuroectodermal differentiation of pluripotent P19 embryonal carcinoma cells (ECCs). Read More

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A Case of Concurrent Disseminated Coccidioidomycosis and Embryonal Carcinoma When Lice and Fleas Coexist.

J Investig Med High Impact Case Rep 2022 Jan-Dec;10:23247096221098339

Department of Internal Medicine, Division of Infectious Diseases, UCLA at Kern Medical Center, Bakersfield, CA, USA.

Coccidioidomycosis (CM) is a fungal infection endemic to the southwestern United States with a wide range of clinical presentations depending on the infected organ systems. Most infections are asymptomatic. Coccidioidomycosis causes a primary pulmonary infection and when symptoms occur, they most often resemble community-acquired pneumonia. Read More

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Prdm12 regulates inhibitory neuron differentiation in mouse embryonal carcinoma cells.

Cytotechnology 2022 Apr 2;74(2):329-339. Epub 2022 Feb 2.

Molecular Gene Technics, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka-shi, Fukuoka, 8190395 Japan.

The epigenetic regulatory system significant influences the fate determination of cells during developmental processes. Prdm12 is a transcriptional regulator that modulates gene expression epigenetically. The gene has been shown to be expressed in neural tissues, specifically during development, but its detailed function is not fully understood. Read More

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Subfamily-specific differential contribution of individual monomers and the tether sequence to mouse L1 promoter activity.

Mob DNA 2022 Apr 20;13(1):13. Epub 2022 Apr 20.

Department of Pharmaceutical Sciences, South Dakota State University, Brookings, SD, 57007, USA.

Background: The internal promoter in L1 5'UTR is critical for autonomous L1 transcription and initiating retrotransposition. Unlike the human genome, which features one contemporarily active subfamily, four subfamilies (A_I, Gf_I and Tf_I/II) have been amplifying in the mouse genome in the last one million years. Moreover, mouse L1 5'UTRs are organized into tandem repeats called monomers, which are separated from ORF1 by a tether domain. Read More

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Morphological spectrum and molecular features of somatic malignant transformation in germ cell tumours.

Histopathology 2022 Jul 5;81(1):84-98. Epub 2022 May 5.

Department of Pathology and Molecular Pathology, University Hospital Zurich, Schmelzbergstrasse 12, Zurich, Switzerland.

Aims: Somatic malignant transformation (SMT) arising in germ cell tumours (GCTs) is an infrequent, but clinically relevant event. There is only limited knowledge on the morphological spectrum of SMT, and the therapeutic management of these patients is poorly defined. In this work we revisit two consecutive case series (n = 756) of GCTs. Read More

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An unusual testicular mass in a young male: Metastasis from occult gastric carcinoma.

Indian J Pathol Microbiol 2022 Apr-Jun;65(2):440-443

Department of Pathology, All India Institute of Medical Sciences, New Delhi, India.

Metastases to the testis are uncommon. Signet-ring cell carcinomas from the gastrointestinal tract (GIT) can rarely disseminate to the testicles, mimicking primary testicular malignancies with signet-ring cells. We hereby describe a case of a 26-year-old male who presented with left testicular swelling, multiple lymphadenopathies, and normal serum tumor markers. Read More

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RANKL regulates testicular cancer growth and Denosumab treatment has suppressive effects on GCNIS and advanced seminoma.

Br J Cancer 2022 Apr 13. Epub 2022 Apr 13.

Group of Skeletal, Mineral, and Gonadal Endocrinology, Department of Growth and Reproduction, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark.

Background: Testicular germ cell tumours (TGCTs) have a high sensitivity to chemotherapy and a high cure rate, although with serious adverse effects. In the search for tumour suppressive drugs, the RANKL inhibitor Denosumab, used to treat osteoporosis, came up as a candidate since RANKL signalling was recently identified in the testis.

Methods: Expression of RANKL, RANK and OPG, and the effects of RANKL inhibition were investigated in human TGCTs, TGCT-derived cell-lines, and TGCT-xenograft models. Read More

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Differential expression of preferentially expressed antigen in melanoma (PRAME) in testicular germ cell tumors - A comparative study with SOX17.

Exp Mol Pathol 2022 Jun 4;126:104761. Epub 2022 Apr 4.

Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455, USA; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA. Electronic address:

The accurate identification of different components in testicular germ cell tumors (GCT) is essential for tailoring treatment and informing the clinical prognosis. PRAME (preferentially expressed antigen in melanoma), a member in the family of cancer testis antigens, plays critical roles in regulating pluripotency and suppressing somatic/germ cell differentiation in seminomas (SEM). To investigate the potential diagnostic value of PRAME in testicular GCT, here we comparatively examined the expression patterns of PRAME and SOX17 by immunohistochemistry in both pure and mixed GCT. Read More

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Single-course bleomycin, etoposide, and cisplatin (1xBEP) as adjuvant treatment in testicular nonseminoma clinical stage 1: outcome, safety, and risk factors for relapse in a population-based study.

Ther Adv Med Oncol 2022 31;14:17588359221086813. Epub 2022 Mar 31.

Department of Medical Oncology, Wilhelminenspital, Vienna, Austria.

Introduction: Clinical stage 1 (CS1) nonseminomatous (NS) germ cell tumors involve a 30% probability of relapse upon surveillance. Adjuvant chemotherapy with one course of bleomycin, etoposide, and cisplatin (1xBEP) can reduce this risk to <5%. However, 1xBEP results are based solely on five controlled trials from high-volume centers. Read More

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Metastatic germ cell tumour in effusion cytology.

Cytopathology 2022 Jul 9;33(4):493-498. Epub 2022 Apr 9.

Department of Cytology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Background: Germ cell tumours infrequently metastasise to body cavities, where early detection on fluid samples is possible and can spearhead early treatment and survival.

Materials And Methods: A total of seven cases of metastatic germ cell tumours were retrieved out of 7500 effusion samples received for cytopathological examination from 2015 to 2021. Detailed cytological features of metastatic germ cell tumours in effusion samples were studied, along with a correlation between clinical, radiological, and histopathological features. Read More

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In high-grade ovarian carcinoma, platinum-sensitive tumor recurrence and acquired-resistance derive from quiescent residual cancer cells that overexpress CRYAB, CEACAM6, and SOX2.

J Pathol 2022 Jul 20;257(3):367-378. Epub 2022 Apr 20.

IRCM U1194, INSERM, ICM, University of Montpellier, Montpellier, France.

Most high-grade ovarian carcinomas (HGOCs) are sensitive to carboplatin (CBP)-based chemotherapy but frequently recur within 24 months. Recurrent tumors remain CBP-sensitive and acquire resistance only after several treatment rounds. Recurrences arise from a small number of residual tumor cells not amenable to investigation in patients. Read More

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MAPK activation drives male and female mouse teratocarcinomas from late primordial germ cells.

J Cell Sci 2022 04 20;135(8). Epub 2022 Apr 20.

Department of Biomedicine and Prevention, University of Rome Tor Vergata, Rome, Italy.

Germ cell tumors (GCTs) are rare tumors that can develop in both sexes, peaking in adolescents. To understand the mechanisms that underlie germ cell transformation, we established a GCT mouse model carrying a germ-cell-specific BRafV600E mutation with or without heterozygous Pten deletion. Both male and female mice developed monolateral teratocarcinomas containing embryonal carcinoma (EC) cells that showed an aggressive phenotype and metastatic ability. Read More

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Overview of the 2022 WHO Classification of Thyroid Neoplasms.

Endocr Pathol 2022 Mar 14;33(1):27-63. Epub 2022 Mar 14.

Department of Pathology, University Health Network, University of Toronto, Toronto, ON, Canada.

This review summarizes the changes in the 5th edition of the WHO Classification of Endocrine and Neuroendocrine Tumors that relate to the thyroid gland. The new classification has divided thyroid tumors into several new categories that allow for a clearer understanding of the cell of origin, pathologic features (cytopathology and histopathology), molecular classification, and biological behavior. Follicular cell-derived tumors constitute the majority of thyroid neoplasms. Read More

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Bisphenol A induces apoptosis in response to DNA damage through c-Abl/YAP/ p73 pathway in P19 embryonal carcinoma stem cells.

Toxicology 2022 03 24;470:153138. Epub 2022 Feb 24.

College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, Shanxi 030801, China. Electronic address:

Bisphenol A (2,2-bis(4'-hydroxyphenyl) propane, BPA) is a well-known endocrine-disrupting compound that is widely used in various daily products and exhibits embryonic development toxicity and genotoxicity. However, the affected signaling pathways involved in embryonic development especially the interactions of involved proteins remain unclear. In our previous study (Ge et al. Read More

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New insights in gastrointestinal "pediatric" neoplasms in adult patients: pancreatoblastoma, hepatoblastoma and embryonal sarcoma of the liver. A practical approach by GIPPI-GIPAD Groups.

Pathologica 2022 Feb;114(1):64-78

Department of Pathology, Azienda Ospedaliera Universitaria di Padova, Padova, Italy.

Pediatric solid neoplasms are rare and very different from those observed in adults. The majority of them are referred to as embryonal because they arise as a result of alterations in the processes of organogenesis or normal growth and are characterized by proliferation of primitive cells, reproducing the corresponding tissue at various stages of embryonic development. This review will focus on embryonal gastrointestinal pediatric neoplasms in adult patients, including pancreatoblastoma, hepatoblastoma, and embryonal sarcoma of the liver. Read More

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February 2022

Roles of Tumor Markers in Central Nervous System Germ Cell Tumors Revisited with Histopathology-Proven Cases in a Large International Cohort.

Cancers (Basel) 2022 Feb 15;14(4). Epub 2022 Feb 15.

Department of Neurologic Surgery, Mayo Clinic, Rochester, MN 55905, USA.

The central nervous system germ cell tumor (CNS GCT) is a rare and incompletely understood disease. A major outstanding question in the 2015 consensus document for CNS GCT management was the utility and interpretation of the tumor markers human chorionic gonadotropin (HCG) and alpha fetoprotein (AFP) in the diagnosis of malignant non-germinomatous GCTs (hereafter NGGCTs) prior to treatment. In the current study, we assembled two geographically and ethnically different clinical cohorts from the Mayo Clinic (1988-2017) and the intracranial GCT Genome Analysis Consortium (iGCT Consortium) in Japan to address this question. Read More

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February 2022

Non-Epithelial Ovarian Cancers: How Much Do We Really Know?

Int J Environ Res Public Health 2022 01 19;19(3). Epub 2022 Jan 19.

Department of Medical Oncology, Medway NHS Foundation Trust, Windmill Road, Kent ME7 5NY, UK.

Non-epithelial ovarian cancers (NEOC) are a group of uncommon malignancies that mainly includes germ cell tumours (GCT), sex cord-stromal tumours (SCST), and some extremely rare tumours, such as small cell carcinomas and sarcomas. Each of these classifications encompasses multiple histologic subtypes. The aetiology and molecular origins of each sub-group of NEOC require further investigation, and our understanding on the genetic changes should be optimised. Read More

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January 2022

Management of Residual Disease After First-line Chemotherapy in a Patient With a Nonseminomatous Germ Cell Tumor.

Oncology (Williston Park) 2021 12 17;35(12):816-819. Epub 2021 Dec 17.

Hematology-Oncology Department, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.

The case An 18-year-old male presented with a one-month history of a nonpainful right testicular enlargement. He had no family history of neoplasia, nor any relevant past medical history. The physical examination was only remarkable for an enlarged right testicle. Read More

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December 2021

Rare "Burned-Out" Testicular Mixed Germ Cell Tumor on 18F-FDG PET/CT.

Clin Nucl Med 2022 Jan 25. Epub 2022 Jan 25.

From the Department of Nuclear Medicine and PET Center, China Medical University Hospital, Taichung City Department of Nuclear Medicine, National Taiwan University Hospital School of Medicine, National Taiwan University, Taipei City Graduate Institute of Biomedical Sciences, China Medical University Center of Augmented Intelligence in Healthcare, China Medical University Hospital Department of Bioinformatics and Medical Engineering, Asia University Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung City, Taiwan.

Abstract: Spontaneous regression of testicular mixed germ cell tumor is rare and is also called burned-out testicular tumor. We herein present the case of a 20-year-old man who was initially diagnosed with metastatic embryonal carcinoma. 18F-FDG PET/CT demonstrated apparent metastases in the lymph node regions and both lungs. Read More

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January 2022

Selective elimination of pluripotent stem cells by PIKfyve specific inhibitors.

Stem Cell Reports 2022 02 20;17(2):397-412. Epub 2022 Jan 20.

National Institute of Child Health & Human Development, National Institutes of Health, Bldg. 6A/3A15, 6 Center Drive, Bethesda, MD 20892-2790, USA. Electronic address:

Inhibition of PIKfyve phosphoinositide kinase selectively kills autophagy-dependent cancer cells by disrupting lysosome homeostasis. Here, we show that PIKfyve inhibitors can also selectively eliminate pluripotent embryonal carcinoma cells (ECCs), embryonic stem cells, and induced pluripotent stem cells under conditions where differentiated cells remain viable. PIKfyve inhibitors prevented lysosome fission, induced autophagosome accumulation, and reduced cell proliferation in both pluripotent and differentiated cells, but they induced death only in pluripotent cells. Read More

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February 2022

MicroRNA 630 Represses NANOG Expression through Transcriptional and Post-Transcriptional Regulation in Human Embryonal Carcinoma Cells.

Int J Mol Sci 2021 Dec 21;23(1). Epub 2021 Dec 21.

Healthy Aging Research Center, Chang Gung University, Taoyuan 333, Taiwan.

The pluripotent transcription factor NANOG is essential for maintaining embryonic stem cells and driving tumorigenesis. We previously showed that PKC activity is involved in the regulation of NANOG expression. To explore the possible involvement of microRNAs in regulating the expression of key pluripotency factors, we performed a genome-wide analysis of microRNA expression in the embryonal carcinoma cell line NT2/D1 in the presence of the PKC activator, PMA. Read More

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December 2021

piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation.

Mol Neurobiol 2022 Feb 4;59(2):1285-1300. Epub 2022 Jan 4.

Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, 4 Medical Drive, Singapore, 117594, Singapore.

piRNAs (PIWI-interacting RNAs) are a class of small non-coding RNAs (ncRNAs) abundantly expressed in germline cells and involved in suppressing the transposon activity. Interestingly, recent studies have found piRNA expression in the central nervous system (CNS), yet the underlying biological significance remains largely unknown. In this study, we investigated the expression and function of piRNAs during the retinoic acid (RA)-mediated neuronal differentiation in NT2 cells, a human embryonal carcinoma cell line. Read More

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February 2022