6,549 results match your criteria Embryonal Carcinoma


Comparison of fatty acid synthase and cyclooxygenase-2 immunoexpression in embryonal, benign, and malignant odontogenic tissues.

Oral Surg Oral Med Oral Pathol Oral Radiol 2018 Dec 31. Epub 2018 Dec 31.

Molecular Pathology Area, School of Dentistry, Universidad de La Republica (UDELAR), Montevideo, Uruguay.

Objectives: The aim of this study was to analyze the immunohistochemical expression of fatty acid synthase (FASN) and cyclooxygenase-2 (COX-2) in tooth germ (TG), ameloblastoma (AM), ameloblastic carcinoma (AC), ameloblastic fibroma (AF), and ameloblastic fibrosarcoma (AFS).

Study Design: Immunohistochemistry for FASN and COX-2 was performed in 10 TG, 44 AM, 10 AC, 9 AF, and 5 AFS specimens. The results were analyzed by using the immunoreactive score (IRS) and Kruskal-Wallis test followed by Dunn's post-test. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.oooo.2018.12.020DOI Listing
December 2018
1 Read

Unedited microneurosurgery of a mixed germ cell tumor of the pineal region.

Surg Neurol Int 2018 24;9:262. Epub 2018 Dec 24.

International Center for Neurosurgery, Henan Provincial People's Hospital, Zhengzhou, China.

Background: Germ cell tumors comprise a heterogeneous group of neoplasms, classified as germinomas and nongerminomatous germ cell tumors based on clinicopathological features. The nongerminomatous group of tumors includes embryonal carcinoma, endodermal sinus tumor (yolk sac tumor), choriocarcinoma, mature and immature teratoma, and mixed germ cell tumors with more than one element. While germinomas are radiation-sensitive tumors, all other tumors have less response to radiotherapy, and it is suggested that gross total resection improves their overall survival and tumor-free survival rates. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4103/sni.sni_357_18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322164PMC
December 2018
1 Read

Human testis phosphoproteome reveals kinases as potential targets in spermatogenesis and testicular cancer.

Mol Cell Proteomics 2019 Jan 25. Epub 2019 Jan 25.

Lead Pharma BV, Netherlands

Spermatogenesis is a complex cell differentiation process that includes marked genetic, cellular, functional and structural changes. It requires tight regulation, since disturbances in any of the spermatogenic processes would lead to fertility deficiencies as well as disorders in offspring. In order to increase our knowledge of signal transduction during sperm development, we carried out a large-scale identification of the phosphorylation events that occur in the human male gonad. Read More

View Article

Download full-text PDF

Source
http://www.mcponline.org/lookup/doi/10.1074/mcp.RA118.001278
Publisher Site
http://dx.doi.org/10.1074/mcp.RA118.001278DOI Listing
January 2019
3 Reads

SGTb regulates a surface localization of a guidance receptor BOC to promote neurite outgrowth.

Cell Signal 2019 Mar 9;55:100-108. Epub 2019 Jan 9.

Department of Molecular Cell Biology, Single Cell Network Research Center, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of Korea; Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Republic of Korea. Electronic address:

Neuritogenesis is a critical event for neuronal differentiation and neuronal circuitry formation during neuronal development and regeneration. Our previous study revealed a critical role of a guidance receptor BOC in a neuronal differentiation and neurite outgrowth. However, regulatory mechanisms for BOC signaling pathway remain largely unexplored. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cellsig.2019.01.003DOI Listing
March 2019
3 Reads

New Anti-Cancer Strategies in Testicular Germ Cell Tumors.

Recent Pat Anticancer Drug Discov 2019 Jan 11. Epub 2019 Jan 11.

Istituto di Endocrinologia ed Oncologia Sperimentale del CNR c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli #39;Federico II', Naples. Italy.

Background: The most common solid malignancy of young men aged 20 to 34 years is testicular germ cell tumor. In addition, the incidence of these tumors has significatively increased throughout the last years. Testicular germ cell tumors are classified into seminoma and nonseminoma germ cell tumors, which take in yolk sac tumor, embryonal cell carcinoma, choriocarcinoma, and teratoma. Read More

View Article

Download full-text PDF

Source
http://www.eurekaselect.com/168893/article
Publisher Site
http://dx.doi.org/10.2174/1574892814666190111120023DOI Listing
January 2019
14 Reads

MicroRNA-135a-5p promotes neuronal differentiation of pluripotent embryonal carcinoma cells by repressing Sox6/CD44 pathway.

Biochem Biophys Res Commun 2019 Feb 31;509(2):603-610. Epub 2018 Dec 31.

From the Department of Molecular Neurobiology, Shaanxi Normal University, Xi'an, 710119, China. Electronic address:

MicroRNA-135a-5p has been reported to play a potential role in the generation of new neurons. However, the underlying targets of miR-135a-5p in regulating neuronal differentiation have been poorly understood. Our study recently has uncovered that Sox6 and CD44 genes were significantly downregulated during neuronal differentiation of P19 cells, a multipotent cell type. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrc.2018.12.162DOI Listing
February 2019
1 Read
2.297 Impact Factor

TAp73 Modifies Metabolism and Positively Regulates Growth of Cancer Stem-Like Cells in a Redox-Sensitive Manner.

Clin Cancer Res 2018 Dec 28. Epub 2018 Dec 28.

Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada.

Stem-like cancer cells, with characteristic self-renewal abilities, remain highly refractory to various clinical interventions. As such, stemness-inhibiting entities, such as tumor suppressor p53, are therapeutically pursued for their anticancer activities. Interestingly, similar implications for tumor suppressor TAp73 in regulating stemness features within stem-like cancer cells remain unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1158/1078-0432.CCR-17-3177DOI Listing
December 2018
3 Reads

Incidence of testicular tumor subtypes according to the updated WHO classification, North Rhine-Westphalia, Germany, 2008-2013.

Andrology 2018 Dec 21. Epub 2018 Dec 21.

Cancer Registry of North Rhine-Westphalia, Muenster, Germany.

Background: In 2016, the WHO introduced an updated classification for testicular tumors. The application of this updated classification to cancer registry data requires some recoding of tumors.

Objectives: The aim of this study was to provide up-to-date population-based incidence estimates of subtypes of testicular germ cell tumors (TGCT) according to the updated classification. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/andr.12565DOI Listing
December 2018
1 Read

5-Azacitidine Exerts Prolonged Pro-Apoptotic Effects and Overcomes Cisplatin-Resistance in Non-Seminomatous Germ Cell Tumor Cells.

Int J Mol Sci 2018 Dec 21;20(1). Epub 2018 Dec 21.

Department of Oncology, Hematology and Bone Marrow Transplantation with Division of Pneumology, University Medical Center Eppendorf, 20246 Hamburg, Germany.

Despite high cure rates, about 20% of patients with advanced germ cell tumors (GCTs) fail cisplatin-based chemotherapy. High levels of DNA methylation have been identified in GCTs and linked to cisplatin resistance. Here, we examined the effects of DNA hypomethylating 5-azacitidine (5-aza) on two embryonal carcinoma cell lines (NCCIT, 2102Ep) and their cisplatin-resistant isogenic derivatives. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms20010021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337423PMC
December 2018
1 Read

Dynamic spatiotemporal patterns of alternative splicing of an F-actin scaffold protein, afadin, during murine development.

Gene 2019 Mar 17;689:56-68. Epub 2018 Dec 17.

Laboratory of Cell and Molecular Biology, Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan; Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan. Electronic address:

An F-actin scaffold protein, afadin, comprises two splice variants called l-afadin (a long isoform) and s-afadin (a short isoform). It is known that in adult tissues, l-afadin is ubiquitously expressed while s-afadin is restrictedly expressed in brain. In cultured cortical neurons, l-afadin potentiates axonal branching whereas s-afadin blocks axonal branching by functioning as a naturally occurring dominant-negative isoform that forms a heterodimer with l-afadin. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gene.2018.12.020DOI Listing
March 2019
1 Read

Correlation Between the Sites of Onset of Basal Cell Carcinoma and the Embryonic Fusion Planes in the Auricle.

Clin Med Insights Oncol 2018 7;12:1179554918817328. Epub 2018 Dec 7.

Advanced Technologies for Regenerative Medicine and Inductive Surgery Research Center, University of Pavia, Pavia, Italy.

Objectives: This study aims at the identification of the distribution of basal cell carcinomas (BCCs) in the auricle in correlation with the currently most credited sites of the embryonic fusion planes of the auricle.

Methods: An overall number of 69 patients with 72 BCCs of the auricle were enrolled in the study over a period of 14 years, from June 2003 to October 2017. All the cases underwent medical preoperative digital photography and the specific location of each BCC was coded on an original full-size anatomical diagram of the auricle derived from the reports by Streeter, Wood-Jones, Park, Porter, and Minoux showing the currently most credited sites of the embryonic fusion planes arbitrarily featured as two 5-mm-wide ribbon-like areas: (1) the hyoid-mandibular fusion plane (HM-FP) running from the upper margin of the tragus toward the concha and then deflecting toward the lower margin of the tragus and (2) the free ear fold-hyoid fusion plane (FEFH-FP) running from the cranial-most portion of the helix to the mid-portion of the ascending helix. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1177/1179554918817328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293364PMC
December 2018
7 Reads

Neuroglial Differentiation and Neoplasms in Testicular Germ Cell Tumors Lack Immunohistochemical Evidence of Alterations Characteristic of Their CNS Counterparts: A Study of 13 Cases.

Am J Surg Pathol 2019 Mar;43(3):422-431

Departments of Pathology.

Overgrowth of neuroglial tissue is rare in testicular germ cell tumors and mostly reported as isolated cases. We retrospectively reviewed 13 cases of testicular germ cell tumors from 2 institutions from 1995 to 2018. Hematoxylin and eosin slides were collected and reviewed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/PAS.0000000000001206DOI Listing
March 2019
3 Reads

Giving Birth After Fertility Sparing Treatment of Embrional Carcinoma Figo III C: Case Report and Literature Review.

Med Arch 2018 Nov;72(5):371-373

Department for Pathology, Policlinic for Laboratory Diagnostic, University Clinical Center Tuzla, Tuzla, Bosnia and Herzegovina.

Introduction: Malignant ovarian germ cell tumors (MOGCTs) account for 2-5% of all ovarian cancers and among them pure embryonal cell cancer is rare condition (1, 2, 3, 4).

Aim: To show successful pregnancy after unilateral salpingooopherectomy and chemotherapy in a girl with embryonal carcinoma of ovary (ECO).

Case Report: Patient had FIGO stage III c disease. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.5455/medarh.2018.72.371-373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282920PMC
November 2018
2 Reads

Clinicopathological Significance and Antitumor Effect of MPHOSPH1 in Testicular Germ Cell Tumor.

J Cancer 2018 31;9(23):4440-4448. Epub 2018 Oct 31.

Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.

MPHOSPH1, which is one of the kinesin superfamily proteins, has been reported to play an essential role in the carcinogenesis and progression of several kinds of cancers. MPHOSPH1 has also been suggested to be involved in STAT3 phosphorylation in hepatocellular carcinoma. However, the biological behavior of MPHOSPH1 in testicular germ cell tumors (TGCTs) is unclear at present. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.7150/jca.25279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277652PMC
October 2018
2 Reads

Embryonal Origin of Metanephric Adenoma and its Differential Diagnosis.

Anticancer Res 2018 Dec;38(12):6663-6667

Department of Urology, Medical School, University of Pecs, Pecs, Hungary

Background/aim: The association of Wilms' tumor (WT), papillary renal cell tumor (PRCT) and mucinous tubular and spindle cell carcinoma (MTSCC) with embryonal rests has already been documented, but the cellular origin of metanephric adenoma (MA) is not yet known. The aim of this study was to understand their developmental evolution and find diagnostic markers.

Materials And Methods: CD57, KRT7, AMACR, SCEL, WT1 and CDH17 expression was analysed by immunohistochemistry in the four types of tumors and the associated pre-neoplastic lesions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.21873/anticanres.13033DOI Listing
December 2018
1 Read

The MicroRNA-371 Family as Plasma Biomarkers for Monitoring Undifferentiated and Potentially Malignant Human Pluripotent Stem Cells in Teratoma Assays.

Stem Cell Reports 2018 Dec 29;11(6):1493-1505. Epub 2018 Nov 29.

Department of Pathology, Laboratory for Experimental Patho-Oncology, Erasmus MC Cancer Institute, Be-432A, PO Box 2040, 3000 CA Rotterdam, the Netherlands. Electronic address:

Predicting developmental potency and risk of posttransplantation tumor formation by human pluripotent stem cells (hPSCs) and their derivatives largely rely on classical histological analysis of teratomas. Here, we investigated whether an assay based on microRNAs (miRNA) in blood plasma is able to detect potentially malignant elements. Several hPSCs and human malignant germ cell tumor (hGCT) lines were investigated in vitro and in vivo after mouse xenografting. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S22136711183044
Publisher Site
http://dx.doi.org/10.1016/j.stemcr.2018.11.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294243PMC
December 2018
7 Reads

CD147 Is a Novel Chemotherapy or Prevention Target in Melanoma.

J Investig Dermatol Symp Proc 2018 Dec;19(2):S91-S93

The Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, China; Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, Hunan, China. Electronic address:

CD147, also named as BSG, was first identified from F9 embryonal carcinoma cells (Miyauchi et al., 1990) and the human BSG locus on chromosome 19p13.3 containing 10 exons (Belton et al. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S10870024183001
Publisher Site
http://dx.doi.org/10.1016/j.jisp.2018.10.001DOI Listing
December 2018
13 Reads

Intraarterial indocyanine green (ICG) fluorescence augmentation by marking embryonal resection areas in colorectal surgery: a feasibility study in a porcine model.

Minim Invasive Ther Allied Technol 2018 Nov 15:1-5. Epub 2018 Nov 15.

a Department of General, Visceral, and Transplantation Surgery, Hospital of the LMU Munich , Ludwig-Maximilians-University (LMU) , Munich , Germany.

Aim: In this pilot animal study we examined whether it is possible to visualize the embryonal resection layers by using intraarterial indocyanine green (ICG) staining when performing total mesorectal excision (TME) for carcinoma of the rectum.

Material And Methods: We injected ICG into the inferior mesenteric artery (AMI) of four swines to see whether the watershed area of the arterial supply zone can be sufficiently visualized by fluorescence imaging in order to mark the right dissection area along the fascia parietalis before and during resection.

Results: We observed a fluorescence signal in all the supplied areas of AMI but not in other parts of the abdominal cavity or other organs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/13645706.2018.1544568DOI Listing
November 2018
3 Reads

DNA methylation profiling as a tool for testicular germ cell tumors subtyping.

Epigenomics 2018 Nov 12. Epub 2018 Nov 12.

Cancer Biology & Epigenetics Group, IPO Porto Research Center (CI-IPOP), Portuguese Oncology Institute of Porto (IPO Porto), Porto, Portugal.

Aim: Assess differential patterns of selected five genes' promoter methylation among testicular germ cell tumors (TGCT) subtypes.

Materials & Methods:  CRIPTO, HOXA9, MGMT, RASSF1A and SCGB3A1 promoter methylation levels were evaluated by quantitative methylation-specific PCR in 161 TGCT and 16 controls. Associations between clinicopathological parameters and promoter methylation levels were assessed, and receiver operating characteristics curve analysis was performed. Read More

View Article

Download full-text PDF

Source
https://www.futuremedicine.com/doi/10.2217/epi-2018-0034
Publisher Site
http://dx.doi.org/10.2217/epi-2018-0034DOI Listing
November 2018
21 Reads

Malignant Spinal Cord Compression Syndrome as an Initial Presentation of Testicular Cancer.

Case Rep Oncol Med 2018 8;2018:5757434. Epub 2018 Oct 8.

Centro Universitario Contra el Cáncer, University Hospital "Dr. José Eleuterio González" and Faculty of Medicine, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.

Malignant spinal cord compression syndrome (MSCCS) occurs in 2.5 to 5% of all oncological patients. In 20% of the cases, it is the initial manifestation. Read More

View Article

Download full-text PDF

Source
https://www.hindawi.com/journals/crionm/2018/5757434/
Publisher Site
http://dx.doi.org/10.1155/2018/5757434DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6196924PMC
October 2018
18 Reads

Overexpression of the Pluripotent Stem Cell Marker Podocalyxin in Prostate Cancer.

Anticancer Res 2018 Nov;38(11):6361-6366

Department of Pathology, Wayne State University School of Medicine, Detroit, MI, U.S.A.

Background/aim: Podocalyxin, a member of the CD34 family of cell surface sialomucins, is overexpressed in human embryonal carcinoma cell lines, as well as in several cancer types, and is associated with poor prognosis. Podocalyxin variants are associated with an increased risk and aggressiveness of prostate cancer. Herein podocalyxin protein expression in prostate cancer was characterized. Read More

View Article

Download full-text PDF

Source
http://ar.iiarjournals.org/lookup/doi/10.21873/anticanres.12
Publisher Site
http://dx.doi.org/10.21873/anticanres.12994DOI Listing
November 2018
15 Reads

RHOA activity in expanding blastocysts is essential to regulate HIPPO-YAP signaling and to maintain the trophectoderm-specific gene expression program in a ROCK/actin filament-independent manner.

Mol Hum Reprod 2019 Feb;25(2):43-60

Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA.

Study Question: What molecular signals are required to maintain the functional trophectoderm (TE) during blastocyst expansion of the late stage of preimplantation development?

Summary Answer: The activity of ras homology family member A (RHOA) GTPases is necessary to retain the expanded blastocyst cavity and also to sustain the gene expression program specific to TE.

What Is Known Already: At the early stages of preimplantation development, the precursor of the TE lineage is generated through the molecular signals that integrate RHOA, RHO-associated coiled-coil containing protein kinase (ROCK), the apicobasal cell polarity, and the HIPPO-Yes-associated protein (YAP) signaling pathway. By contrast, molecular mechanisms regulating the maintenance of the TE characteristics at the later stage, which is crucial for blastocyst hatching and implantation, are scarcely understood. Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/molehr/advance-article/doi/10.1093/
Publisher Site
http://dx.doi.org/10.1093/molehr/gay048DOI Listing
February 2019
5 Reads

Aryl hydrocarbon receptor mediates the cardiac developmental toxicity of EOM from PM in P19 embryonic carcinoma cells.

Chemosphere 2019 Feb 23;216:372-378. Epub 2018 Oct 23.

Medical College of Soochow University, Suzhou, China. Electronic address:

Ambient fine particulate matter (PM) has been found to be associated with congenital heart defects, but the molecular mechanisms remain to be elucidated. Our previous study revealed that extractable organic matter (EOM) from PM exerted cardiac developmental toxicity in zebrafish embryos. The aim of the current study is to explore the effects of EOM on cardiac differentiation of P19 mouse embryonic carcinoma stem cells. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00456535183202
Publisher Site
http://dx.doi.org/10.1016/j.chemosphere.2018.10.160DOI Listing
February 2019
2 Reads

CK7, inhibin, and p63 in testicular germ cell tumor: superior markers of Choriocarcinoma compared to β-hCG.

Hum Pathol 2018 Oct 22. Epub 2018 Oct 22.

Department of Pathology, The Ohio State University Medical Center, 410 W 10(th) Ave., 401 Doan Hall, Columbus, OH 43210. Electronic address:

Choriocarcinoma can be difficult to differentiate from other subtypes of testicular germ cell tumor and can occur unexpectedly in a distant, late metastasis. The aim of this investigation was to identify a marker superior to β-hCG for choriocarcinoma. 62 primary and metastatic testicular germ cell tumors (27 choriocarcinomas, 19 yolk sac tumors, 29 embryonal carcinomas, 28 seminomas, 22 teratomas, 3 epithelioid trophoblastic tumors (ETT)), were analyzed for immunohistochemical expression of CK7, inhibin, p63, and β-hCG. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00468177183039
Publisher Site
http://dx.doi.org/10.1016/j.humpath.2018.10.007DOI Listing
October 2018
9 Reads
2.770 Impact Factor

Malignant mixed ovarian germ cell tumor composed of immature teratoma, yolk sac tumor and embryonal carcinoma harboring an EGFR mutation: a case report.

Onco Targets Ther 2018 12;11:6853-6862. Epub 2018 Oct 12.

Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China,

Malignant mixed ovarian germ cell tumors are very rare, accounting for ~5.3% of all malignant ovarian germ cell tumors (MOGCTs), with a very high degree of malignancy. The treatment of patients with persistent, refractory, or platinum-resistant MOGCT is not well defined. Read More

View Article

Download full-text PDF

Source
https://www.dovepress.com/malignant-mixed-ovarian-germ-cell-
Publisher Site
http://dx.doi.org/10.2147/OTT.S176854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190639PMC
October 2018
4 Reads

Metabolic profile and differentiation potential of extraembryonic endoderm-like cells.

Cell Death Discov 2018 26;4:42. Epub 2018 Sep 26.

1Department of Biology, Collaborative Graduate Specialization in Developmental Biology, The University of Western Ontario, London, ON Canada.

Glucose metabolism has a crucial role for providing substrates required to generate ATP and regulate the epigenetic landscape. We reported that F9 embryonal carcinoma stem-like cells require cytosolic reactive oxygen species to differentiate into extraembryonic endoderm; however, mitochondrial sources were not examined. To extend these studies, we examined the metabolic profile of early and late-passage F9 cells, and show that their ability to differentiate is similar, even though each population has dramatically different metabolic profiles. Read More

View Article

Download full-text PDF

Source
http://www.nature.com/articles/s41420-018-0102-1
Publisher Site
http://dx.doi.org/10.1038/s41420-018-0102-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158286PMC
September 2018
13 Reads

Clinicopathological Characteristics of Urinary Bladder Tumors in Korean Patients 20 Years or Younger.

J Korean Med Sci 2018 Oct 13;33(40):e242. Epub 2018 Aug 13.

Department of Urology, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Korea.

Background: To investigate the clinicopathological characteristics of urinary bladder tumors, a rare malignancy, in patients 20 years or younger.

Methods: Using a retrospective chart review among patients who received bladder surgery at 2 institutions between July 1996 and January 2013, we analyzed the clinicopathological characteristics of urinary bladder tumors in 21 pediatric patients (male:female = 4.25:1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3346/jkms.2018.33.e242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159107PMC
October 2018
4 Reads

Seminoma component of mixed testicular germ cell tumor shows a higher incidence of loss of heterozygosity than pure-type seminoma.

Hum Pathol 2018 Sep 25. Epub 2018 Sep 25.

Department of Basic Pathology, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.

Using analysis of allelic loss [loss of heterozygosity (LOH)], we previously reported a putative progression pathway from germ cell neoplasia in situ (GCNIS) to seminoma, and then to embryonal carcinoma in mixed-type testicular germ cell tumors. To identify the genetic backgrounds related to the progression of non-seminomatous germ cell tumor (NSGCT), patterns of LOH were studied in seminoma components in mixed tumors (18 cases), pure seminomas (20 cases), and co-existing GCNIS lesions. Each tumor was assessed for LOH at 22 polymorphic loci located on 12 chromosomal arms: 3q, 5q, 6p, 9p, 10q, 11p, 12p, 12q, 13q, 17p, 17q, and 18q. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.humpath.2018.09.007DOI Listing
September 2018
2 Reads

Phosphoglycerate dehydrogenase inhibition induces p-mTOR-independent autophagy and promotes multilineage differentiation in embryonal carcinoma stem-like cells.

Cell Death Dis 2018 Sep 24;9(10):990. Epub 2018 Sep 24.

Department of Pathology, Dalhousie University, Halifax, NS, Canada.

Cancer cells with a less differentiated stem-like phenotype are more resistant to therapeutic manipulations than their differentiated counterparts, and are considered as one of the main causes of cancer persistence and relapse. As such, induction of differentiation in cancer stem-like cells (CSLCs) has emerged as an alternative strategy to enhance the efficacy of anticancer therapies. CSLCs are metabolically distinct from differentiated cells, and any aberration from the intrinsic metabolic state can induce differentiation of CSLCs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-018-0997-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155240PMC
September 2018
7 Reads

Human Endogenous Retrovirus-K HML-2 integration within is associated with intravenous drug abuse and modulates transcription in a cell-line model.

Proc Natl Acad Sci U S A 2018 10 24;115(41):10434-10439. Epub 2018 Sep 24.

Department of Zoology, University of Oxford, Oxford OX1 3PS, United Kingdom;

HERV-K HML-2 (HK2) has been proliferating in the germ line of humans at least as recently as 250,000 years ago, with some integrations that remain polymorphic in the modern human population. One of the solitary HK2 LTR polymorphic integrations lies between exons 17 and 18 of , a gene that affects dopaminergic activity and is thus related to addiction. Here we show that this antisense HK2 integration (namely RASGRF2-int) is found more frequently in persons who inject drugs compared with the general population. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.1811940115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187174PMC
October 2018
3 Reads

The association between sex and most childhood cancers is not mediated by birthweight.

Cancer Epidemiol 2018 Dec 21;57:7-12. Epub 2018 Sep 21.

Division of Epidemiology & Clinical Research, Department of Pediatrics, University of Minnesota, Minneapolis, MN, United States; Masonic Cancer Center, University of Minnesota, Minneapolis, MN, United States. Electronic address:

Background: Male sex is associated with an increased risk of childhood cancer as is high birthweight. Given that sex determination precedes birthweight we conducted a mediation analysis to estimate the direct effect of sex in association with childhood cancer tumor type with birthweight as the mediator.

Methods: Cases (n = 12,632) and controls (n = 64,439) (ages 0-14 years) were identified from population-based cancer and birth registries in Minnesota, New York, and Washington states (1970-2014). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.canep.2018.09.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289597PMC
December 2018
4 Reads

P19 Cells as a Model for Studying the Circadian Clock in Stem Cells before and after Cell Differentiation.

J Circadian Rhythms 2018 May 18;16. Epub 2018 May 18.

Medical Core Facility and Research Platforms, King Abdullah International Medical Research Center/King Saud bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs, Riyadh 11426, SA.

In mammals, circadian rhythmicity is sustained via a transcriptional/translational feedback loop referred to as the canonical molecular circadian clock. Circadian rhythm is absent in undifferentiated embryonic stem cells; it begins only after differentiation. We used pluripotent P19 embryonal carcinoma stem cells to check the biological clock before and after differentiation into neurons using retinoic acid. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.5334/jcr.157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6083773PMC
May 2018
2 Reads

Benzothiazole carbamates and amides as antiproliferative species.

Eur J Med Chem 2018 Sep 29;157:1096-1114. Epub 2018 Aug 29.

Serbian Academy of Sciences and Arts, Knez Mihailova 35, 11158 Belgrade, Serbia; University of Belgrade, Faculty of Chemistry, Studentski Trg 16, P.O. Box 51, 11158 Belgrade, Serbia. Electronic address:

A series of new benzothiazole-based carbamates and amides were synthesized and their antiproliferative activity was determined. Derivatives with profound activity were identified and further investigated for their possible mechanism of action. It was found that these compounds induce specific apoptosis, G2/M cell cycle arrest and decrease ROS level in MCF-7 human breast cancer cell line. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejmech.2018.08.067DOI Listing
September 2018
15 Reads

Testis-sparing surgery: Experience in 13 patients with oncologic and functional outcomes.

Can Urol Assoc J 2018 08 30. Epub 2018 Aug 30.

Koc University, School of Medicine, Department of Urology, Istanbul.

Introduction: We present oncological and functional outcomes of patients who underwent testis-sparing surgery (TSS).

Methods: Overall, 13 patients were included. Mean patient age was 29. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.5489/cuaj.5379DOI Listing
August 2018
4 Reads

LncRNA-uc.40 silence promotes P19 embryonic cells differentiation to cardiomyocyte via the PBX1 gene.

In Vitro Cell Dev Biol Anim 2018 Sep 15;54(8):600-609. Epub 2018 Aug 15.

Department of Pediatrics, Changzhou Children's Hospital, Nantong Medical University, Nantong City, China.

Uc.40 is a long noncoding RNA that is highly conserved among different species, although its function is unknown. It is highly expressed in abnormal human embryonic heart. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s11626-018-0284-0
Publisher Site
http://dx.doi.org/10.1007/s11626-018-0284-0DOI Listing
September 2018
8 Reads

CD30-Redirected Chimeric Antigen Receptor T Cells Target CD30 and CD30 Embryonal Carcinoma via Antigen-Dependent and Fas/FasL Interactions.

Cancer Immunol Res 2018 Oct 7;6(10):1274-1287. Epub 2018 Aug 7.

Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.

Tumor antigen heterogeneity limits success of chimeric antigen receptor (CAR) T-cell therapies. Embryonal carcinomas (EC) and mixed testicular germ cell tumors (TGCT) containing EC, which are the most aggressive TGCT subtypes, are useful for dissecting this issue as ECs express the CD30 antigen but also contain CD30 cells. We found that CD30-redirected CAR T cells (CD30. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1158/2326-6066.CIR-18-0065DOI Listing
October 2018
2 Reads

A Comparison of the Histopathologic Growth Patterns Between Non-Merkel Cell Small Round Blue Cell Tumors and Merkel Cell Carcinoma.

Am J Dermatopathol 2018 Nov;40(11):815-818

Department of Dermatology, Medical University of South Carolina, Charleston, SC.

Background: Merkel cell carcinoma (MCC) is a rare neuroendocrine cutaneous malignancy that shares cytologic, histopathologic, and immunohistochemical features with other small round blue cell (SRBC) tumors. Although the trabecular pattern is anecdotally associated with MCC, objective data are lacking.

Methods: This was a retrospective institutional review board-approved observational study conducted on microscopic images of 79 MCCs and 74 other SRBC tumors (desmoplastic small round cell tumor, primitive neuroectodermal tumor, neuroblastoma, embryonal rhabdomyosarcoma, alveolar rhabdomyosarcoma, synovial sarcoma, carcinoid, metastatic small cell lung cancer, non-Hodgkin small cell lymphoma, retinoblastoma, medulloblastoma, nephroblastoma, small cell osteosarcoma, and round cell liposarcoma). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/DAD.0000000000001232DOI Listing
November 2018
9 Reads
1.430 Impact Factor

Testicular germ cell tumors: revisiting a series in light of the new WHO classification and AJCC staging systems, focusing on challenges for pathologists.

Hum Pathol 2018 Dec 21;82:113-124. Epub 2018 Jul 21.

Department of Pathology, Portuguese Oncology Institute of Porto (IPOP), R. Dr. António Bernardino de Almeida, 4200-072, Porto, Portugal; Cancer Biology and Epigenetics Group, Research Center of Portuguese Oncology Institute of Porto (GEBC CI-IPOP), R. Dr. António Bernardino de Almeida, 4200-072, Porto, Portugal; Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar, University of Porto (ICBAS-UP), Rua Jorge Viterbo Ferreira 228, 4050-513, Porto, Portugal. Electronic address:

Testicular germ cell tumors (TGCTs) are strikingly heterogeneous, reflecting a complex tumor model, posing serious challenges for pathologists. Accurate classification and staging, according to most recent systems, is fundamental. We aimed to revise a series of consecutively diagnosed TGCTs (2005-2016) in light of the new World Health Organization (WHO) classification and American Joint Committee on Cancer (AJCC) staging systems, discussing dilemmas imposed to pathologists. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.humpath.2018.07.016DOI Listing
December 2018
11 Reads

Genomic functions of developmental pluripotency associated factor 4 (Dppa4) in pluripotent stem cells and cancer.

Stem Cell Res 2018 08 19;31:83-94. Epub 2018 Jul 19.

Department of Cell Biology and Human Anatomy, University of California, Davis, 1 Shields Ave, Davis, CA 95616, United States.; Institute of Pediatric Regenerative Medicine, Shriners Hospitals for Children Northern California, Sacramento, CA 95817, United States. Electronic address:

Developmental pluripotency associated factor 4 (Dppa4) is a highly specific marker of pluripotent cells, and is also overexpressed in certain cancers, but its function in either of these contexts is poorly understood. In this study, we use ChIP-Seq to identify Dppa4 binding genome-wide in three distinct cell types: mouse embryonic stem cells (mESC), embryonal carcinoma cells, and 3T3 fibroblasts ectopically expressing Dppa4. We find a core set of Dppa4 binding sites shared across cell types, and also a substantial number of sites unique to each cell type. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scr.2018.07.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6133722PMC
August 2018
20 Reads

Directed differentiation of mouse P19 embryonal carcinoma cells to neural cells in a serum- and retinoic acid-free culture medium.

In Vitro Cell Dev Biol Anim 2018 Sep 20;54(8):567-579. Epub 2018 Jul 20.

Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Sir CV Raman Road, Bangalore, 560012, India.

P19 embryonal carcinoma cells (EC-cells) provide a simple and robust culture system for studying neural development. Most protocols developed so far for directing neural differentiation of P19 cells depend on the use of culture medium supplemented with retinoic acid (RA) and serum, which has an undefined composition. Hence, such protocols are not suitable for many molecular studies. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11626-018-0275-1DOI Listing
September 2018
2 Reads

Analysis of the Paternally-Imprinted DLK1-MEG3 and IGF2-H19 Tandem Gene Loci in NT2 Embryonal Carcinoma Cells Identifies DLK1 as a Potential Therapeutic Target.

Stem Cell Rev 2018 12;14(6):823-836

Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 South Floyd Street, Louisville, KY, 40202, USA.

The paternally-imprinted genes insulin-like growth factor 2 (IGF2), H19, delta-like homologue 1 (DLK1), and maternally-expressed gene 3 (MEG3) are expressed from the tandem gene loci IGF2-H19 and DLK1-MEG3, which play crucial roles in initiating embryogenesis and development. The erasure of imprinting (EOI) at differentially methylated regions (DMRs) which regulate the expression of these genes maintains the developmental quiescence of primordial germ cells (PGCs) migrating through the embryo proper during embryogenesis and prevents them from forming teratomas. To address the potential involvement of the IGF2-H19 and DLK1-MEG3 loci in the pathogenesis of embryonal carcinoma (EC), we investigated their genomic imprinting at DMRs in the human PGC-derived EC cell line NTera-2 (NT2). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12015-018-9838-5DOI Listing
December 2018
6 Reads

Differentiation-Dependent Regulation of Human Endogenous Retrovirus K Sequences and Neighboring Genes in Germ Cell Tumor Cells.

Front Microbiol 2018 15;9:1253. Epub 2018 Jun 15.

Department of Surgical and Conservative Paediatrics and Adolescent Medicine, Martin Luther University Halle-Wittenberg, Halle, Germany.

Under physiological conditions, most human endogenous retroviruses (HERVs) are transcriptionally silent. However, re-activation of HERVs is observed under pathological conditions like inflammation or cancer. In addition to expression of HERV sequences, an impact of HERV-loci on expression of adjacent genes has been suggested as probably important patho-physiological mechanism. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2018.01253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013571PMC
June 2018
29 Reads

SC1 inhibits the differentiation of F9 embryonic carcinoma cells induced by retinoic acid.

Acta Biochim Biophys Sin (Shanghai) 2018 Aug;50(8):793-799

College of Veterinary Medicine, Northwest A&F University, Yangling, China.

The ability to self-renew is one of the most important properties of embryonic stem (ES) cells. Pluripotin (SC1), a small molecule with high activity and low toxicity, promotes self-renewal in mouse ES cells. SC1 can noticeably change the morphology of retinoic acid (RA)-induced F9 embryonic carcinoma cells (F9 cells). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/abbs/gmy069DOI Listing
August 2018
2 Reads

Recalling Cohnheim's Theory: Papillary Renal Cell Tumor as a Model of Tumorigenesis from Impaired Embryonal Differentiation to Malignant Tumors in Adults.

Int J Biol Sci 2018 21;14(7):784-790. Epub 2018 May 21.

Department of Urology, Medical School, University of Pecs, Hungary.

We have suggested that papillary renal cell tumor (PRCT) of the kidney arises from nephrogenic rest-like lesions. To approve our hypothesis, we worked up 14 kidneys bearing papillary and 14 ones with conventional renal cell carcinoma (CRCC) histologically and found 42 papillary lesions in average per kidney bearing PRCT. PRCTs are characterized by loss of the Y chromosome and trisomy of chromosomes 7 and 17. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.7150/ijbs.22489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001684PMC
May 2018
5 Reads

The combined effects of three-dimensional cell culture and natural tissue extract on neural differentiation of P19 embryonal carcinoma stem cells.

J Tissue Eng Regen Med 2018 Sep 13;12(9):1909-1924. Epub 2018 Aug 13.

Adelaide Medical School, The University of Adelaide, Adelaide, SA, Australia.

Tissue engineering, as a novel transplantation therapy, aims to create biomaterial scaffolds resembling the extracellular matrix in order to regenerate the damaged tissues. Adding bioactive factors to the scaffold would improve cell-tissue interactions. In this study, the effect of chitosan polyvinyl alcohol nanofibres containing carbon nanotube scaffold with or without active bioglass (BG /BG ), in combination with neonatal rat brain extract on cell viability, proliferation, and neural differentiation of P19 embryonic carcinoma stem cells was investigated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/term.2712DOI Listing
September 2018
50 Reads

Integrated Molecular Characterization of Testicular Germ Cell Tumors.

Cell Rep 2018 Jun;23(11):3392-3406

Department of Genetics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Electronic address:

We studied 137 primary testicular germ cell tumors (TGCTs) using high-dimensional assays of genomic, epigenomic, transcriptomic, and proteomic features. These tumors exhibited high aneuploidy and a paucity of somatic mutations. Somatic mutation of only three genes achieved significance-KIT, KRAS, and NRAS-exclusively in samples with seminoma components. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.celrep.2018.05.039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6075738PMC
June 2018
83 Reads

Clinical experience on diagnosis and treatment for malignancy originating from the dacryocyst.

Eye (Lond) 2018 09 30;32(9):1519-1522. Epub 2018 May 30.

Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China.

Objective: To summarize clinical experience on the diagnosis and treatment for malignancy originating from the dacryocyst.

Methods: We retrospectively analyzed clinicopathological data from 12 cases that were diagnosed with malignancy originating from the dacryocyst by histopathological examination in our hospital from 2007 to 2017.

Results: Of the 12 cases with malignancy originating from the dacryocyst, 7 were male and 5 were female, with a mean age of 53 years (range, 4-81). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41433-018-0132-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137217PMC
September 2018
4 Reads