11,965 results match your criteria Electrophoresis [Journal]


The staining efficiency of cyanine dyes for single-stranded DNA is enormously dependent on nucleotide composition.

Electrophoresis 2019 Apr 20. Epub 2019 Apr 20.

College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, P. R. China.

The staining of nucleic acids with fluorescent dyes is one of the most fundamental technologies in relevant areas of science. For reliable and quantitative analysis, the staining efficiency of the dyes should not be very dependent on the sequences of the specimens. However, this assumption has not necessarily been confirmed by experimental results, especially in the staining of ssDNA (and RNA). Read More

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http://dx.doi.org/10.1002/elps.201800445DOI Listing

Authors' correction for "Decision support algorithm for the selection of analytical methods in organic compounds detection for future extraterrestrial exploratory missions".

Electrophoresis 2019 Apr 20. Epub 2019 Apr 20.

Department of Environmental Engineering, Universidad Politécnica Salesiana, Quito, 170801, Ecuador.

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http://dx.doi.org/10.1002/elps.201900159DOI Listing

Preparation of a capillary isoelectric focusing column with monolithic immobilized pH gradient and its application on protein separation based on an online capillary isoelectric focusing platform.

Electrophoresis 2019 Apr 16. Epub 2019 Apr 16.

School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China.

In the presence of methanol and n-decanol as porogens, a partially filled capillary monolithic column was prepared by in situ reaction of glycidyl methacrylate and poly (ethylene glycol) diacrylate. Then, Pharmalyte 3-10 was immobilized on this column in order to obtain a capillary isoelectric focusing (cIEF) column with monolithic immobilized pH gradient (M-IPG). In addition, an online self-built platform for protein separation was established on account of the introduction of a cross-shaped unit and two short-off valves. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.2019001
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http://dx.doi.org/10.1002/elps.201900125DOI Listing
April 2019
1 Read

Electrophoretic transport and dynamic deformation of bio-vesicles.

Electrophoresis 2019 Apr 16. Epub 2019 Apr 16.

Department of Mechanical Engineering, Southern Methodist University, Dallas, TX.

Study of the deformation dynamics of cells and other sub-micron vesicles, such as virus and neurotransmitter vesicles are necessary to understand their functional properties. This mechanical characterization can be done by submerging the vesicle in a fluid medium and deforming it with a controlled electric field, which is known as electrodeformation. Electrodeformation of biological and artificial lipid vesicles is directly influenced by the vesicle and surrounding media properties and geometric factors. Read More

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http://dx.doi.org/10.1002/elps.201900025DOI Listing

A fully portable microchip real-time PCR for rapid detection of pathogen.

Electrophoresis 2019 Apr 12. Epub 2019 Apr 12.

The Nano Science and Technology (NSNT) Program, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, S. A. R. China.

Point-of-care detection for pathogen is of critical need for wide epidemic warning and medical diagnosis. In this work, we designed and developed a fully portable and integrated microchip based real-time PCR machine for rapid pathogen detection. The instrument consists of three functional components including heating, optical and electrical modules which are integrated into a portable compact box. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.2019000
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http://dx.doi.org/10.1002/elps.201900090DOI Listing
April 2019
2 Reads

Development and Validation of a capillary electrophoresis method applied to the analysis of L-Citrulline in an oral formulation for pediatric use.

Electrophoresis 2019 Apr 12. Epub 2019 Apr 12.

Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Tecnología Farmacéutica, Argentina.

A simple and highly sensitive CE-UV method applied to the determination of L-Citrulline was developed from an oral formulation for pedriatric use. The novel method was based on the analysis of L-Citrulline for direct ultraviolet detection at 198 nm. The BGE consisted of 10 mM sodium tetraborate and 50 mM SDS at pH 9, and the electrophoretic parameters were optimized. Read More

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http://dx.doi.org/10.1002/elps.201900008DOI Listing

Carrier ampholyte-free free-flow isoelectric focusing for separation of protein.

Electrophoresis 2019 Apr 12. Epub 2019 Apr 12.

Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, P. R. China.

Free-flow isoelectric focusing (FFIEF) has the merits of mild separation conditions, high recovery and resolution, but suffers from the issues of ampholytes interference and high cost due to expensive carrier ampholytes. In this paper, a home-made carrier ampholyte-free FFIEF system was constructed via orientated migration of H and OH provided by electrode solutions. When applying an electric field, a linear pH gradient from pH 4 to pH 9 (R = 0. Read More

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http://dx.doi.org/10.1002/elps.201900148DOI Listing

Aptamer functionalized magnetic graphene oxide nanocomposites for highly selective capture of histones.

Electrophoresis 2019 Apr 11. Epub 2019 Apr 11.

CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, P. R. China.

The binding coverage of aptamer was an important restricted factor for aptamer-based affinity enrichment strategy for capturing target molecular. Herein, we designed and prepared aptamer functionalized graphene oxide based nanocomposites (GO/NH -NTA/Fe O /PEI/Au), and the coverage density of aptamer was high to 33.1 nmol/mg. Read More

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http://dx.doi.org/10.1002/elps.201900061DOI Listing
April 2019
1 Read

2D nanomaterial-based separation membranes.

Electrophoresis 2019 Apr 10. Epub 2019 Apr 10.

Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen, 361005, P. R. China.

2D nanomaterials including graphene, hexagonal boron-nitride (h-BN), molybdenum disulfide (MoS ), etc., provide immense potentials for separation applications. However, the trade-off between selectivity and permeability in choosing 2D nanomaterial-based membrane is inevitable, limiting the progress on separation efficiency for mass industrial applications. Read More

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http://dx.doi.org/10.1002/elps.201800529DOI Listing
April 2019
3.028 Impact Factor

Il n'y a pas d'amour heureux pour Casanova: Chemical- and bio-analysis of his Memoirs.

Electrophoresis 2019 Apr 8. Epub 2019 Apr 8.

Department of Chemistry, Materials and Chemical Engineering ''Giulio Natta'', Politecnico di Milano, Milano, Italy.

The original manuscript of Casanova's Memoirs is stored at the Bibliothèque Nationale de France in Paris. We have gained access to it and explored the surfaces of chapters one and two (via the ethylene vinyl acetate [EVA] film technology, i.e. Read More

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http://dx.doi.org/10.1002/elps.201800505DOI Listing
April 2019
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Thermodynamic parameters for the complexation of water-insoluble betulin derivatives with (2-hydroxypropyl)-γ-cyclodextrin determined by phase-solubility technique combined with capillary zone electrophoresis.

Electrophoresis 2019 Apr 8. Epub 2019 Apr 8.

Institute of Chemistry and Chemical Technology SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russia.

The complexation of betulinic and betulonic acids (BIA and BOA) (pentacyclic lupane triterpenoids) with (2-hydroxypropyl)-γ-cyclodextrin (HP-γ-CD) was studied at different temperatures using the method combining phase-solubility technique and CZE. In contrast to mobility shift ACE utilizing the electrophoretic mobility, in this approach, the peak areas are used. The apparent binding (stability, formation) constants are obtained by the Higuchi and Connors method from the linear segment of compound solubility diagrams in CD solutions. Read More

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http://dx.doi.org/10.1002/elps.201800516DOI Listing
April 2019
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Biomimetic preparation of core-shell structured SERS substrate with antifouling ability, good stability and reliable quantitative capability.

Electrophoresis 2019 Apr 5. Epub 2019 Apr 5.

Laboratory of Nanoscale Biochemical Analysis, Institute of Functional Nano & Soft Materials (FUNSOM), and Collaborative Innovation Center of Suzhou Nano Science and Technology (NANO-CIC), Soochow University, Suzhou, Jiangsu, 215123, P. R. China.

The fouling and stability are two most critical limiting factors for practical applications of surface-enhanced Raman scattering (SERS)-based microfluidic electrophoresis device. Herein, we present a novel biomimetic nanoengineering strategy to achieve a SERS substrate featuring antifouling ability, good stability and reliable quantitative capability. Typically, by employing tea polyphenol as the reducing agent, the substrate made of silver core-gold shell nanostructures in situ grown on silicon wafer surface is fabricated. Read More

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http://dx.doi.org/10.1002/elps.201800538DOI Listing

Retention prediction using quantitative structure-retention relationships combined with the Hydrophobic Subtraction Model in reversed-phase liquid chromatography.

Electrophoresis 2019 Apr 5. Epub 2019 Apr 5.

Australian Centre for Research on Separation Science (ACROSS), School of Physical Sciences-Chemistry, University of Tasmania, Hobart, Australia.

The Hydrophobic Subtraction Model (HSM) combined with Quantitative Structure-Retention Relationships (QSRR) methodology was utilized to predict retention times in reversed-phase liquid chromatography (RPLC). A selection of new analytes and new RPLC columns that had never been used in the QSRR modeling process were used to verify the proposed approach. This work is designed to facilitate early prediction of co-elution of analytes in pharmaceutical drug discovery applications where it is advantageous to predict whether impurities might be co-eluted with the active drug component. Read More

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http://dx.doi.org/10.1002/elps.201900022DOI Listing
April 2019
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Nanoscaled porphyrinic metal-organic framework for photodynamic/photothermal therapy of tumor.

Electrophoresis 2019 Apr 5. Epub 2019 Apr 5.

State Key Laboratory of Electroanalytical Chemistry, Chinese Academy of Sciences, Changchun Institute of Applied Chemistry, Changchun, P. R. China.

Nanoagents achieving photodynamic therapy (PDT) and photothermal therapy (PTT) combination treatment with improved therapeutic effect are highly desirable. However, the incorporation of both PDT and PTT into a single nanoagent often requires multistep fabrication process. Herein, we report that photoactive porphyrin ligands have been successfully introduced into Zn-TCPP structure to construct the nanoagents that possesses photodynamic performance and photothermal performance simultaneously. Read More

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http://dx.doi.org/10.1002/elps.201900005DOI Listing
April 2019
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Implementation of at-line capillary zone electrophoresis for fast and reliable determination of adenovirus concentrations in vaccine manufacturing.

Electrophoresis 2019 Apr 5. Epub 2019 Apr 5.

Kantisto BV, Baarn, The Netherlands.

A CZE method was validated and implemented for fast and accurate in-process determination of adenovirus concentrations of downstream process samples obtained during manufacturing of adenovirus vector-based vaccines. An analytical-quality-by-design approach was embraced for method development, method implementation, and method maintenance. CZE provided separation of adenovirus particles from sample matrix components, such as cell debris, residual DNA and proteins. Read More

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http://dx.doi.org/10.1002/elps.201900068DOI Listing

In situ fabrication of microporous organic network coated capillary column for high resolution gas chromatographic separation of hydrocarbons.

Electrophoresis 2019 Apr 3. Epub 2019 Apr 3.

State Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, P. R. China.

Microporous organic networks (MONs) are a new class of porous materials synthesized via Sonogashira coupling reactions between organic building blocks. Here we report an in situ synthesis approach to fabricate MONs coated capillary column for high resolution GC separation of hydrocarbons. The McReynolds constant evaluation reveals the MONs coated capillary is a non-polar column. Read More

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http://dx.doi.org/10.1002/elps.201800540DOI Listing

Sensitive amperometric detection for capillary electrophoresis of phenol carbamates with in-line thermal hydrolysis strategy.

Electrophoresis 2019 Mar 29. Epub 2019 Mar 29.

Institute of Technology for Food Safety and Environmental Monitoring, Fuzhou University, Fuzhou, P. R. China.

A strategy on amperometric detection (AD) for capillary zone electrophoresis (CZE) of phenol carbamates as model analytes with a facile in-line thermal hydrolysis was presented, in which a thermal hydrolysis, subsequent CZE separation and final column-end amperometric detection were accomplished in an intact capillary. Key parameters of hydrolysis dynamics of carbamates and electrochemical detection of the hydrolysates were studied, as well as electrophoretic conditions. Under the optimal conditions, the capillary was utilized as chambers for in-situ hydrolysis, CZE separation, and electrochemical detection. Read More

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http://dx.doi.org/10.1002/elps.201800484DOI Listing

Quantitative analysis of microcystin variants by capillary electrophoresis mass spectrometry with dynamic pH barrage junction focusing.

Electrophoresis 2019 Mar 29. Epub 2019 Mar 29.

Department of Chemistry, University of British Columbia, Vancouver, BC, Canada.

Dynamic pH junction is an online focusing method in CE based on the electrophoretic mobility difference of analytes in the sample matrix and the background electrolyte. An advantage of this method over the conventional CE is that the sensitivity can be significantly improved. By injecting a long sample plug in the capillary and focusing the analytes at the pH boundary between the background electrolyte and sample matrix, the LOD can be improved by 10-100 folds. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.2019000
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http://dx.doi.org/10.1002/elps.201900042DOI Listing
March 2019
2 Reads

Development of a novel multiplex polymerase chain reaction system for forensic individual identification using insertion/deletion polymorphisms.

Electrophoresis 2019 Mar 27. Epub 2019 Mar 27.

Key laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, P. R. China.

Insertion/deletion (InDel) polymorphisms have been widely used in the fields of population genetics, genetic map constructions, and forensic investigations owing to the advantages of their low mutation rates, widespread distributions in the human genome, and small amplicon sizes. In order to provide more InDels with high discrimination power in Chinese populations, we selected and constructed one novel multiplex PCR-InDel panel for forensic individual identification. Genetic distributions of these 35 InDels in five reference populations from East Asia showed low genetic differentiations among these populations. Read More

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http://dx.doi.org/10.1002/elps.201800412DOI Listing

On-chip electromembrane extraction of acidic drugs.

Electrophoresis 2019 Mar 27. Epub 2019 Mar 27.

Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

In the present work, a new supported liquid membrane (SLM) has been developed for on-chip electromembrane extraction of acidic drugs combined with HPLC or CE, providing significantly higher stability than those reported up to date. The target analytes are five widely used non-steroidal anti-inflammatory drugs (NSAIDs): ibuprofen (IBU), diclofenac (DIC), naproxen (NAX), ketoprofen (KTP) and salicylic acid (SAL). Two different microchip devices were used, both consisted basically of two poly(methyl methacrylate) (PMMA) plates with individual channels for acceptor and sample solutions, respectively, and a 25 μm thick porous polypropylene membrane impregnated with the organic solvent in between. Read More

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http://dx.doi.org/10.1002/elps.201900024DOI Listing
March 2019
3 Reads

Low-voltage efficient electroosmotic pumps with ultrathin silica nanoporous membrane.

Electrophoresis 2019 Mar 27. Epub 2019 Mar 27.

Institute of Analytical Chemistry, Department of Chemistry, Zhejiang University, Hangzhou, P. R. China.

In this work, an efficient electroosmotic pump (EOP) based on the ultrathin silica nanoporous membrane (u-SNM), which can drive the motion of fluid under the operating voltage as low as 0.2 V, has been fabricated. Thanks to the ultrathin thickness of u-SNM (∼75 nm), the effective electric field strength across u-SNM could be as high as 8. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.2018005
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http://dx.doi.org/10.1002/elps.201800533DOI Listing
March 2019
2 Reads

Detection of structured single-strand DNA via solid-state nanopore.

Electrophoresis 2019 Mar 26. Epub 2019 Mar 26.

Key Laboratory for Advanced Materials, School of Chemistry and Molecular Engineering East, East China University of Science and Technology, Shanghai, P. R. China.

Nanopore as a single-molecule analysis method which also employed electrophoresis has achieved promising single-molecule detections. In this study, we designed two kinds of confined spaces by fabricating solid-state nanopores with desirable diameters to study the structured single-strand DNA of C-rich quadruplex. For the nanopore whose diameter is larger than the quadruplex size, the DNA molecule could directly translocate across the nanopore with extremely high speed. Read More

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http://dx.doi.org/10.1002/elps.201900096DOI Listing
March 2019
1 Read

An improved hybrid continuum-atomistic four-way coupled model for electrokinetics in nanofluidics.

Electrophoresis 2019 Mar 22. Epub 2019 Mar 22.

Mechanical Engineering Department, Amirkabir University of Technology, Tehran, Iran.

In this study, an efficient hybrid continuum-atomistic method is proposed to study electrokinetic transport of aqueous solutions in nanofluidics. The aqueous phase is considered as a continuous phase containing immersed ion particles. The behavior of the system is then simulated through utilization of an improved hybrid continuum-atomistic four-way coupled approach, including the MultiPhase Particle-In-Cell method for the short-ranged interaction between the ion particles, the Brownian force for the collision between the aqueous phase molecules and the ion particles, and a wall force accounting for the short-ranged interaction of ions and walls. Read More

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http://dx.doi.org/10.1002/elps.201800307DOI Listing

Separation of brombuterol enantiomers in capillary electrophoresis with cyclodextrin-type chiral selectors and investigation of structure of selector-selectand complexes using nuclear magnetic resonance spectroscopy.

Electrophoresis 2019 Mar 21. Epub 2019 Mar 21.

Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi, Georgia.

The major goal of this study was to determine the affinity pattern of brombuterol (BB) enantiomers toward various cyclodextrins (CD) and to evaluate the potential of NMR spectroscopy for understanding fine mechanisms of interactions between CDs and BB enantiomers. Separation of BB enantiomers was performed in a fused-silica capillary using a phosphate buffer, pH 2.5, at the room temperature in the normal polarity mode. Read More

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http://dx.doi.org/10.1002/elps.201900062DOI Listing
March 2019
3.028 Impact Factor

Enantioselective capillary electrophoresis for pharmacokinetic analysis of methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine in equines anesthetized with ketamine and isoflurane.

Electrophoresis 2019 Mar 22. Epub 2019 Mar 22.

Clinical Pharmacology Laboratory, Institute for Infectious Diseases, University of Bern, Bern, Switzerland.

An enantioselective assay for the determination of methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine in equine plasma based on capillary electrophoresis with highly sulfated γ-cyclodextrin as chiral selector and electrokinetic analyte injection is described. The assay is based on liquid/liquid extraction of the analytes at alkaline pH from 0.1 mL plasma followed by electrokinetic sample injection of the analytes from the extract across a buffer plug without chiral selector. Read More

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http://dx.doi.org/10.1002/elps.201900044DOI Listing

Droplet-based microfluidics systems in biomedical applications.

Electrophoresis 2019 Mar 20. Epub 2019 Mar 20.

School of Information and Communication Engineering, University of Electronic Science and Technology of China, Chengdu, P. R. China.

Microfluidics has made a very impressive progress in the past decades due to its unique and instinctive advantages. Droplet-based microfluidic systems show excellent compatibility with many chemical and biological reagents and are capable of performing variety of operations that can implement microreactor, complex multiple core-shell structure, and many applications in biomedical research such as drug encapsulation, targeted drug delivery systems, and multifunctionalization on carriers. Droplet-based systems have been directly used to synthesize particles and encapsulate many biological entities for biomedicine applications due to their powerful encapsulation capability and facile versatility. Read More

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http://dx.doi.org/10.1002/elps.201900047DOI Listing

Coupling of capillary electrophoresis with electrospray ionization multiplexing ion mobility spectrometry.

Electrophoresis 2019 Mar 20. Epub 2019 Mar 20.

College of Chemical Engineering, Xiangtan University, Xiangtan, Hunan, P. R. China.

In this work, ion mobility spectrometry (IMS) function as a detector and another dimension of separation was coupled with CE to achieve two-dimensional separation. To improve the performance of hyphenated CE-IMS instrument, electrospray ionization correlation ion mobility spectrometry is evaluated and compared with traditional signal averaging data acquisition method using tetraalkylammonium bromide compounds. The effect of various parameters on the separation including sample introduction, sheath fluid of CE and drift gas, data acquisition method of IMS were investigated. Read More

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http://dx.doi.org/10.1002/elps.201800462DOI Listing
March 2019
1 Read

Simultaneous isolation and label-free identification of bacteria using contactless dielectrophoresis and Raman spectroscopy.

Electrophoresis 2019 Mar 20. Epub 2019 Mar 20.

Utah State University, Department of Biological Engineering, Logan, UT, USA.

The traditional bacterial identification method of growing colonies on agar plates can take several days to weeks to complete depending on the growth rate of the bacteria. Successfully decreasing this analysis time requires cell isolation followed by identification. One way to decrease analysis time is by combining dielectrophoresis (DEP), a common technique used for cell sorting and isolation, and Raman spectroscopy for cell identification. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.2018003
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http://dx.doi.org/10.1002/elps.201800389DOI Listing
March 2019
3 Reads

Synthesis of the chiral selector heptakis(6-O-methyl)-β-cyclodextrin by phase-transfer catalysis and hydrazine-mediated transfer-hydrogenation.

Electrophoresis 2019 Mar 20. Epub 2019 Mar 20.

Department of Pharmacognosy, Semmelweis University, Budapest, Hungary.

The exhaustive primary-side alkylation of cyclodextrins has never been achieved directly. The undesired and simultaneous derivatization of the secondary hydroxyl moieties generates intricate isomeric mixtures that are challenging to purify, analyse and characterize. The aim of this study was to develop a chromatography-free and up-scalable strategy towards the preparation of per-6-O-methylated cyclodextrin and to test the compound as potential chiral selector. Read More

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http://dx.doi.org/10.1002/elps.201900065DOI Listing

Distinct and independent dielectrophoretic behavior of the head and tail of sperm and its potential for the safe sorting and isolation of rare spermatozoa.

Electrophoresis 2019 Mar 20. Epub 2019 Mar 20.

Faculty of Mechanical Engineering, Micro- and Nanofluidics Laboratory, Technion-Israel Institute of Technology, Technion City, Israel.

Often, in semen samples with minute amounts of sperm, even the single spermatozoon required to fertilize an oocyte cannot be found in the ejaculate. This is primarily because currently, sperm is generally searched for manually under a microscope. In this study, dielectrophoresis (DEP) was investigated as an alternative automated technique for sorting sperm cells. Read More

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http://dx.doi.org/10.1002/elps.201800437DOI Listing

Nuclear magnetic resonance to study the interactions acting in the enantiomeric separation of homocysteine by capillary electrophoresis with a dual system of γ-cyclodextrin and the chiral ionic liquid EtCholNTf.

Electrophoresis 2019 Mar 20. Epub 2019 Mar 20.

Departamento de Química Analítica, Química Física e Ingeniería Química. Facultad de Ciencias, Universidad de Alcalá. Ctra., Alcalá de Henares, Madrid, Spain.

The enantiomeric separation of 9-fluorenylmethoxycarbonyl chloride (FMOC)-homocysteine (Hcy) by CE was investigated using γ-CD and the chiral ionic liquid (R)-(1-hydroxybutan-2-yl)(trimethyl)azanium-bis(trifluoromethanesulfon)imidate (also called (R)-N,N,N-trimethyl-2-aminobutanol-bis(trifluoromethane-sulfon)imidate) (EtCholNTf ) as chiral selectors. Using 2 mM γ-CD and 5 mM EtCholNTf in 50 mM borate buffer (pH 9), FMOC-Hcy enantiomers were separated with a resolution value of 3.8. Read More

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http://dx.doi.org/10.1002/elps.201800483DOI Listing

Biomimetic stimuli-responsive nanochannels and their applications.

Authors:
Lulu Fu Jin Zhai

Electrophoresis 2019 Mar 18. Epub 2019 Mar 18.

Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, Beijing Key Laboratory of Bio-inspired Energy Materials and Devices, School of Chemistry, Beihang University, Beijing, P. R. China.

Biomimetic smart nanochannels have been studied extensively to achieve the precise ionic transport compared to biological ion channels. Similar to ion channels in living organisms, biomimetic smart nanochannels can respond to various stimuli, which allows for promising applications in many fields. In this review, we mainly summarize the recent advances in the design of biomimetic stimuli-responsive nanochannels and their potential applications including biosensors and drug delivery. Read More

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http://dx.doi.org/10.1002/elps.201800536DOI Listing

A microscopic physical description of electrothermal-induced flow for control of ion current transport in microfluidics interfacing nanofluidics.

Electrophoresis 2019 Mar 18. Epub 2019 Mar 18.

School of Electronics and Control Engineering, School of Highway, Chang'an University, Xi'an, Shaanxi, P. R. China.

The phenomenon of electrothermal (ET) convection has recently captured great attention for transporting fluidic samples in microchannels embedding simple electrode structures. In the classical model of ET-induced flow, a conductivity gradient of buffer medium is supposed to arise from temperature-dependent electrophoretic mobility of ionic species under uniform salt concentrations, so it may not work well in the presence of evident concentration perturbation within the background electrolyte. To solve this problem, we develop herein a microscopic physical description of ET streaming by fully coupling a set of Poisson-Nernst-Planck-Navier-Stokes equations and temperature-dependent fluid physicochemical properties. Read More

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http://dx.doi.org/10.1002/elps.201900105DOI Listing

Joule heating effects in optimized insulator-based dielectrophoretic devices: An interplay between post geometry and temperature rise.

Electrophoresis 2019 Mar 18. Epub 2019 Mar 18.

Microscale Bioseparations Laboratory, Rochester Institute of Technology, Rochester, NY, USA.

Insulator-based dielectrophoresis (iDEP) is the electrokinetic migration of polarized particles when subjected to a non-uniform electric field generated by the inclusion of insulating structures between two remote electrodes. Electrode spacing is considerable in iDEP systems when compared to electrode-based DEP systems, therefore, iDEP systems require high voltages to achieve efficient particle manipulation. A consequence of this is the temperature increase within the channel due to Joule heating effects, which, in some cases, can be detrimental when manipulating biological samples. Read More

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http://dx.doi.org/10.1002/elps.201800490DOI Listing

Human chorionic gonadotrophin pharmaceutical formulations of urinary origin display high levels of contaminant proteins-A label-free quantitation proteomics study.

Electrophoresis 2019 Mar 18. Epub 2019 Mar 18.

Clinical Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.

To determine whether there is a measurable protein background in different formulations of urinary and recombinant human chorionic gonadotropin (hCG). Primary outcome measures: identification of contaminant proteins in urinary-derived formulations of hCG; secondary outcome measures: quantitative values of contaminant proteins in different batches of urinary -derived hCG formulations. It was found that urinary-derived batches have high presence of contaminant proteins beside the active substance. Read More

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http://dx.doi.org/10.1002/elps.201900087DOI Listing

Immobilizing sulfotransferase 1A1 on magnetic microparticles and their evaluation using capillary electrophoresis.

Electrophoresis 2019 Mar 18. Epub 2019 Mar 18.

KU Leuven-University of Leuven, Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, Leuven, Belgium.

Sulfotransferases are categorized as phase II metabolic enzymes. Human sulfotransferase 1A1 (SULT1A1) is involved in the sulfonation of xenobiotics with aid from the cofactor 3'-phosphoadenosine-5'-phosphosulfate that acts as a sulfonate donor. In this study, we have attempted to immobilize SULT1A1 on magnetic microparticles (MMs). Read More

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http://dx.doi.org/10.1002/elps.201900016DOI Listing
March 2019
1 Read

ICMFLOC 2018.

Authors:

Electrophoresis 2019 Mar;40(6):837

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http://dx.doi.org/10.1002/elps.201970055DOI Listing

Limitations of the Clausius-Mossotti function used in dielectrophoresis and electrical impedance studies of biomacromolecules.

Authors:
Ronald Pethig

Electrophoresis 2019 Mar 12. Epub 2019 Mar 12.

School of Engineering, Institute for Integrated Micro and Nanosystems, University of Edinburgh, Edinburgh, UK.

Dielectrophoresis (DEP) studies have progressed from the microscopic scale of cells and bacteria, through the mesoscale of virions to the molecular scale of DNA and proteins. The Clausius-Mossotti function, based on macroscopic electrostatics, is invariably employed in the analyses of all these studies. The limitations of this practice are explored, with the conclusion that it should be abandoned for the DEP study of proteins and modified for native DNA. Read More

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http://dx.doi.org/10.1002/elps.201900057DOI Listing
March 2019
1 Read

A microchip device to enhance free flow electrophoresis using controllable pinched sample injections.

Electrophoresis 2019 Mar 12. Epub 2019 Mar 12.

Department of Chemistry, Lanzhou University, Lanzhou, Gansu, P. R. China.

Micro free flow electrophoresis (μFFE) is a valuable technique capable of high throughput rapid microscale electrophoretic separation along with mild operating conditions. However, the stream flow separation nature of free flow electrophoresis affects its separation performance with additional stream broadening due to sample stream deflection. To reduce stream broadening and enhance separation performance of μFFE, we presented a simple microfluidic device that enables injection bandwidth control. Read More

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http://dx.doi.org/10.1002/elps.201900079DOI Listing

Direct detection of DNA using 3D surface enhanced Raman scattering hotspot matrix.

Electrophoresis 2019 Mar 12. Epub 2019 Mar 12.

Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, P. R. China.

Silver nanoparticles (AgNPs) are evaporatively self-assembled into the 3D surface enhanced Raman scattering (SERS) hotspot matrix with the assistant of glycerol to improve the spectral reproducibility in direct DNA detection. AgNPs and DNA in the glycerol-stabilized 3D SERS hotspot matrix are found to form flexible sandwich structures through electrostatic interaction where neighboring AgNPs create uniform and homogeneous localized surface plasmon resonance coupling environments for central DNA. Nearly two orders of magnitude extra SERS enhancement, more stable peak frequency and narrower peak full width at half maximum can therefore be obtained in DNA SERS spectra, which ensures highly stable and reproducible SERS signals in direct detection of both single strand DNA and double strand DNA utilizing the 3D SERS hotspot matrix. Read More

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http://dx.doi.org/10.1002/elps.201900009DOI Listing

Application of micellar electrokinetic chromatography for detection of silver nanoparticles released from wound dressing.

Electrophoresis 2019 Mar 8. Epub 2019 Mar 8.

Department of Dermatology, Medical University of Warsaw, Warsaw, Poland.

The recent emergence of nanotechnology has provided a new therapeutic modality in case of silver nanoparticles. Dressings containing silver form the basis for the treatment of burns and wounds, either acute or chronic ones. The aim of the study was to examine silver release from the different wound dressings: commercially available (Atrauman Ag, Aquacel Ag) and experimental (FKDP-AgNPs) using MEKC. Read More

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http://dx.doi.org/10.1002/elps.201900020DOI Listing
March 2019
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Separations of antifungal compounds in capillary electrophoresis with two anionic cyclodextrins.

Electrophoresis 2019 Mar 7. Epub 2019 Mar 7.

Univ. Lille, Inserm, U995 - LIRIC - Lille Inflammation Research International Center, Lille, France.

CD-CZE methods were developed for complete stereoisomeric separations of a series of six γ-lactam analogues, of which some were neutral, or cationic depending on the background electrolyte nature. The tested cyclodextrin was the versatile sulfobutylether- β-CD, used either in a phosphate buffer using capillaries dynamically coated with polyethylene oxide or in a borate buffer using uncoated capillaries. Long-end and short-end modes and concentration variations of chiral selectors allowed finding conditions of complete separation of four out of the six derivatives (i. Read More

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http://dx.doi.org/10.1002/elps.201800479DOI Listing
March 2019
1 Read

Development of a PRiME cartridge purification method for rapid determination of malachite green and leucomalachite green in Chinese softshell turtle.

Electrophoresis 2019 Mar 6. Epub 2019 Mar 6.

Key Laboratory of Anesthesiology of Zhejiang Province, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, P. R. China.

A high-throughput PRiME (process, robustness, improvements, matrix effects, ease of use) sample purification procedure was developed to simplify the multiple steps of traditional SPE in extracting the malachite green and leucomalachite green in Chinese softshell turtle (Pelodiscus sinensis). The sample loading volume, extracting solvent type, and pH value of the employed PRiME hydrophilic-lipophilic balance cartridge for sample purification were optimized to be 3 mL, acetonitrile, and pH 5, respectively. In comparison with traditional SPE, the PRiME process is cost-effective, solvent-saving, and simple to operate, which only consists of a passing through step without traditional sorbent conditioning and impurity washing. Read More

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http://dx.doi.org/10.1002/elps.201900081DOI Listing

Comparison of GC-MS and MEKC methods for caffeine determination in preworkout supplements.

Electrophoresis 2019 Mar 6. Epub 2019 Mar 6.

Department of Analytical Chemistry and Instrumental Analysis, Institute of Sport-National Research Institute, Warsaw, Poland.

In this study, GC-MS- and MEKC-based methods for determination of caffeine (CAF) in preworkout supplements were developed and validated. The proposed protocols utilized minimal sample preparation (simple dilution and syringe filtration). The developed methods achieved satisfactory validation parameters, i. Read More

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http://dx.doi.org/10.1002/elps.201800512DOI Listing
March 2019
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The lift force on a charged sphere that translates and rotates in an electrolyte.

Electrophoresis 2019 Mar 4. Epub 2019 Mar 4.

Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.

The distortion of the charge cloud around a uniformly charged, dielectric, rigid sphere that translates and rotates in an unbounded binary, symmetric electrolyte at zero Reynolds number is examined. The zeta potential of the particle ζ is assumed small relative to the thermal voltage scale. It is assumed that the equilibrium structure of the cloud is slightly distorted, which requires that the Péclet numbers characterizing distortion due to particle translation, , and rotation, , are small compared to unity. Read More

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http://dx.doi.org/10.1002/elps.201900029DOI Listing

Dielectrophoretic cell trapping for improved surface plasmon resonance imaging sensing.

Electrophoresis 2019 Mar 4. Epub 2019 Mar 4.

Laboratoire Nanotechnologies Nanosystèmes (LN2) - CNRS UMI-3463, Université de Sherbrooke, École Centrale de Lyon, Sherbrooke, Canada.

The performance of conventional surface plasmon resonance (SPR) biosensors can be limited by the diffusion of the target analyte to the sensor surface. This work presents an SPR biosensor that incorporates an active mass-transport mechanism based on dielectrophoresis and electroosmotic flow to enhance analyte transport to the sensor surface and reduce the time required for detection. Both these phenomena rely on the generation of AC electric fields that can be tailored by shaping the electrodes that also serve as the SPR sensing areas. Read More

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http://dx.doi.org/10.1002/elps.201800439DOI Listing

Editorial.

Electrophoresis 2019 Mar;40(5):605

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http://dx.doi.org/10.1002/elps.201970047DOI Listing

Capillary electrophoretic assay of human acetyl-coenzyme A carboxylase 2.

Electrophoresis 2019 Mar 3. Epub 2019 Mar 3.

Department of Chemistry, Louisiana State University, Baton Rouge, LA, USA.

Human acetyl-coenzyme A carboxylase 2 catalyzes the carboxylation of acetyl coenzyme A to form malonyl coenzyme A, along with the conversion of magnesium-adenosine triphosphate complex to magnesium-adenosine diphosphate complex. A simple off-column capillary electrophoresis assay for human acetyl-coenzyme A carboxylase 2 was developed based on the separation of magnesium-adenosine triphosphate complex, magnesium-adenosine diphosphate complex, acetyl coenzyme A and malonyl coenzyme A with detection by ultraviolet absorption at 256 nm. When Mg was absent from the separation buffer, the zones due to magnesium-adenosine triphosphate complex and magnesium-adenosine diphosphate complex both split and migrated as two separate peaks. Read More

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http://dx.doi.org/10.1002/elps.201800514DOI Listing

Recent advances in direct current electrokinetic manipulation of particles for microfluidic applications.

Authors:
Xiangchun Xuan

Electrophoresis 2019 Feb 28. Epub 2019 Feb 28.

Department of Mechanical Engineering, Clemson University, Clemson, SC, USA.

Microfluidic devices have been extensively used to achieve precise transport and placement of a variety of particles for numerous applications. A range of force fields have thus far been demonstrated to control the motion of particles in microchannels. Among them, electric field-driven particle manipulation may be the most popular and versatile technique because of its general applicability and adaptability as well as the ease of operation and integration into lab-on-a-chip systems. Read More

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http://dx.doi.org/10.1002/elps.201900048DOI Listing
February 2019
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Highly sensitive and selective separation of intact parathyroid hormone and variants by sheathless CE-ESI-MS/MS.

Electrophoresis 2019 Feb 27. Epub 2019 Feb 27.

Laboratory for the Analysis of Medicines (LAM), Center for Interdisciplinary Research on Medicines (CIRM), University of Liège, Liège, Belgium.

Parathyroid hormone (PTH) is a common clinical marker whose quantification relies on immunoassays, giving variable results as batch, brand, or target epitope changes. Sheathless CE-ESI-MS, combining CE resolution power and low-flow ESI sensitivity, was applied to the analysis of PTH in its native conformation in the presence of related forms. Fused silica and neutral-coated capillaries were investigated, as well as preconcentration methods such as transient isotachophoresis, field-amplified sample injection (FASI), and electrokinetic supercharging (EKS). Read More

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http://dx.doi.org/10.1002/elps.201800507DOI Listing
February 2019