11,907 results match your criteria Electrophoresis [Journal]


Magnetic Digital Microfluidics on a Bioinspired Surface for Point-of-Care Diagnostics of Infectious Disease.

Electrophoresis 2019 Feb 16. Epub 2019 Feb 16.

Singapore 3D Printing Center, School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore.

Magnetic digital microfluidics uses magnetic force to manipulate droplets on a Teflon-coated substrate through the added magnetic particles. To achieve a wide range of droplet manipulation, hydrophilic patterns, known as surface energy traps, are introduced onto the Teflon-coated hydrophobic substrate. However, the Teflon-coated substrate is difficult to modify because it is non-wettable, and existing techniques for patterning surface energy traps have many limitations. Read More

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http://dx.doi.org/10.1002/elps.201900074DOI Listing
February 2019

Editorial.

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Electrophoresis 2019 Feb;40(4):485-486

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http://dx.doi.org/10.1002/elps.201970035DOI Listing
February 2019

Evaluation of ForenSeq™ Signature Prep Kit B on predicting eye and hair coloration as well as biogeographical ancestry by using Universal Analysis Software (UAS) and available web-tools.

Electrophoresis 2019 Feb 15. Epub 2019 Feb 15.

Office of Chief Medical Examiner, Department of Forensic Biology, New York, NY, USA.

This study examined 266 individuals from various populations including African American, East Asian, South Asian, European, and mixed populations to evaluate the ForenSeq™ Signature Prep Kit Primer Mix B. Focus was placed on phenotypic and biogeographical ancestry predictions by Illumina's Universal Analysis Software (UAS). These outcomes were compared to those obtained through web-tools developed at the Erasmus Medical Center (EMC) and available from the Forensic Resource/Reference on Genetics-knowledge base (FROG-kb), as well as to eye color predictions by the 8-plex system. Read More

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http://dx.doi.org/10.1002/elps.201800344DOI Listing
February 2019

Determination of genomic N3-methylthymidine in human cancer cells treated with nitrosamines using capillary electrophoresis with laser-induced fluorescence.

Electrophoresis 2019 Feb 15. Epub 2019 Feb 15.

Division of Radiopharmaceutical Chemistry, Heidelberg, Germany.

Methylating substances alter DNA by forming N3-methylthymidine (N3mT), a mutagenic base modification. To develop a sensitive analytical method for the detection of N3mT in DNA based on capillary electrophoresis with laser-induced fluorescence detection (CE-LIF), we synthesized the N3mT-3'-phosphate as a chemical standard. The limit of detection was 1. Read More

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http://dx.doi.org/10.1002/elps.201800495DOI Listing
February 2019

Electro-osmotic surface effects generation in an electrokinetic-based transport device: A comparison of RF and MW plasma generating sources.

Electrophoresis 2019 Feb 14. Epub 2019 Feb 14.

Department of Chemical and Materials Engineering, University of Idaho, Moscow, United States.

It is a common practice in insulator-based dielectrophoretic separation to use and reuse PDMS-constructed microdevice for an extended period of time while performing biological and technical replicate experiments. This is usually done to rule out any effects of device variation on separation efficiency. Ensuring that all experimental conditions remain the same is critical to the conclusion that can be drawn from such repeated experiments. Read More

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http://dx.doi.org/10.1002/elps.201800464DOI Listing
February 2019

Chiral separation of rasagiline using sulfobutylether-β-cyclodextrin: capillary electrophoresis, NMR and molecular modeling study.

Electrophoresis 2019 Feb 13. Epub 2019 Feb 13.

Department of Pharmaceutical Chemistry, Semmelweis University, Budapest, Hungary.

Pressure-assisted stereospecific capillary electrophoresis method was developed for the determination of enantiomeric purity of the antiparkinsonian agent (R)-rasagiline. The optimized method, 50 mM glycine-HCl buffer pH 2.0, supplied with 30 mM sulfobutylether-β-cyclodextrin, at 35°C, applying 12 kV in reversed polarity, and - 8 mbar pressure (vacuum), short-end injection with -25 mbar x 2 seconds, was successful for baseline separation of rasagiline enantiomers (R = 3. Read More

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http://dx.doi.org/10.1002/elps.201800482DOI Listing
February 2019

Distinguishing three distinct biogeographic regions with an in-house developed 39-AIM-InDel panel and further admixture proportion estimation for Uyghurs.

Electrophoresis 2019 Feb 13. Epub 2019 Feb 13.

Department of Forensic Genetics, School of Forensic Medicine, Southern Medical University, Guangzhou, P. R. China.

In the forensic field, ancestry-informative markers showing remarkable allele frequency discrepancies can be useful in deducing the likely ancestral origin of a person or estimating the ancestry component proportions of admixed populations or individuals. Diallelic SNPs are genetic markers commonly used for ancestry inference, but the genotyping methods of SNPs fail to fulfil the demands of cost-effectiveness and simplicity of experimental manipulation. To overcome the limitations, a 39 ancestry-informative InDels multiplex panel was developed in the present study to perform ancestry assignment of individuals from three distinct biogeographic regions (African, European, East Asian). Read More

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http://doi.wiley.com/10.1002/elps.201800448
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http://dx.doi.org/10.1002/elps.201800448DOI Listing
February 2019
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On the design of deterministic dielectrophoresis for continuous separation of circulating tumor cells from peripheral blood cells.

Electrophoresis 2019 Feb 10. Epub 2019 Feb 10.

Department of Mechanical and Industrial Engineering, University of Illinois at Chicago, Chicago, IL, USA.

Recently, detection and analysis of circulating tumor cells (CTCs) have emerged as a promising way to diagnose cancer, study its cellular mechanism, and test or develop potential treatments. However, the rarity of CTCs among peripheral blood cells is a big challenge towards CTC detection. In addition, in cases where there is similar size range between certain types of CTCs (e. Read More

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http://dx.doi.org/10.1002/elps.201800459DOI Listing
February 2019
1 Read

Analysis and interpretation of mixture DNA using AS-PCR of mtDNA.

Electrophoresis 2019 Feb 10. Epub 2019 Feb 10.

School of Forensic Medicine, China Medical University, Shenyang, P. R. China.

Semi-nested PCR with allele-specific (AS) primers and sequencing of mitochondrial DNA (mtDNA) were performed to analyze and interpret DNA mixtures, especially when biological materials were degraded or contained a limited amount of DNA. SNP-STR markers were available to identify the minor DNA component using AS-PCR; moreover, SNPs in mtDNA could be used when the degraded or limited amounts of DNA mixtures were not successful with SNP-STR markers. Five pairs of allele specific primers were designed based on three SNPs (G15043A, T16362C, and T16519C). Read More

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http://dx.doi.org/10.1002/elps.201800432DOI Listing
February 2019

Enantioseparation of tryptophan and its unnatural derivatives by nano-LC on CSP-teicoplanin silica based.

Electrophoresis 2019 Feb 6. Epub 2019 Feb 6.

Ph.D. School in Natural Science and Engineering, University of Verona, Verona, Italy.

This work deals with the potentiality of nano liquid chromatography (Nano-LC) for the chiral separation of racemic mixture of tryptophan and some selected derivatives by using 100 μm i.d. fused silica capillary packed with teicoplanin bonded to 5 μm diol silica stationary phase. Read More

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http://dx.doi.org/10.1002/elps.201800508DOI Listing
February 2019

Magnetic solid-phase extraction based on carbon nanosphere@Fe O for enantioselective determination of eight triazole fungicides in water samples.

Electrophoresis 2019 Feb 6. Epub 2019 Feb 6.

Lab of Analytical Chemistry, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, P. R. China.

In this work, a carbon nanosphere decorated by Fe O nanoparticles was prepared, characterized and used as the magnetic adsorbent. Eight commonly used chiral triazole fungicides, including penconazole, uniconazole, paclobutrazol, triazolone, tebuconazole, hexaconazole, triticonazole and epoxiconazole were extracted from two environmental water samples (river water and lake water) by magnetic solid-phase extraction, followed by the enantiomeric analysis on a Chiralpark IC column coupled with a triple quadrupole mass spectrometry to evaluate their possible stereoselective degradation occurring in the water samples. The possible factors affecting the extraction performance, such as amount of used adsorbents, pH and ionic strength of water solution, types and volumes of desorption solvents were systematically investigated. Read More

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http://dx.doi.org/10.1002/elps.201800530DOI Listing
February 2019

A sensitive dispersive micro solid-phase extraction coupled with high performance liquid chromatography for determination of three flavonoids in complex matrics by using crab shell as a sorbent.

Electrophoresis 2019 Feb 6. Epub 2019 Feb 6.

College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, P. R. China.

A sensitive dispersive micro solid-phase extraction coupled with high performance liquid chromatography has been developed for preconcentration and determination of three flavonoids (quercetin, kaempferol and isorhamnetin) in complex matrix samples. Parameters that affecting extraction efficiency have been optimized. The optimal extraction conditions is using 2 μg/mL of crab shell as the sorbent, extraction for 2 min at pH 7 and then eluting with 100 μL of methanol. Read More

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http://dx.doi.org/10.1002/elps.201900003DOI Listing
February 2019

Sensitive detection of hydroxymethylcytosine levels in normal and neoplastic cells and tissues.

Electrophoresis 2019 Feb 6. Epub 2019 Feb 6.

Division of Radiopharmaceutical Chemistry, Heidelberg, Germany.

A new sensitive analytical method using capillary electrophoresis with laser induced fluorescence (CE-LIF) was applied for the simultaneous detection of DNA methylation and hydroxymethylation levels in human cancers of different origin. DNA hydroxymethylation, measured as 5-hydroxymethylcytosine (5hmC) levels, was decreased in gliomas with mutation in the isocitrate dehydrogenase 1 (IDH1) gene when compared to IDH1-wildtype gliomas. Independent from IDH1 mutation, 5hmC levels were decreased in lung carcinomas when compared to normal lung tissue. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.2018005
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http://dx.doi.org/10.1002/elps.201800523DOI Listing
February 2019
8 Reads

Recent studies of docking and molecular dynamics simulation for liquid-phase enantioseparations.

Electrophoresis 2019 Feb 2. Epub 2019 Feb 2.

Dipartimento di Scienze Molecolari e Nanosistemi DSMN, Università Ca' Foscari Venezia, Mestre Venezia, Veneto, Italy.

Liquid-phase enantioseparations have been fruitfully applied in several fields of science. Various applications along with technical and theoretical advancements contributed to increase significantly the knowledge in this area. Nowadays, chromatographic techniques, in particular HPLC on chiral stationary phase, are considered as mature technologies. Read More

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http://dx.doi.org/10.1002/elps.201800493DOI Listing
February 2019

Cyclodextrin-mediated capillary electrophoresis enantioseparation of dansylated β-amino acids with bicyclo[2.2.2]octane, bicyclo[3.1.1]heptane and cyclopenta[d][1,2]oxazole core structures.

Electrophoresis 2019 Feb 1. Epub 2019 Feb 1.

Department of Pharmaceutical and Medicinal Chemistry, School of Pharmacy, Friedrich Schiller University Jena, Jena, Germany.

The present study investigated the separation of bicyclic β-amino acids with bicyclo[2.2.2]octane, bicyclo[3. Read More

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http://dx.doi.org/10.1002/elps.201800492DOI Listing
February 2019

Microfluidics and Miniaturization 2019.

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Electrophoresis 2019 Feb;40(3):357

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http://dx.doi.org/10.1002/elps.201970025DOI Listing
February 2019

Erratum.

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Electrophoresis 2019 Feb;40(3):473

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http://dx.doi.org/10.1002/elps.201970027DOI Listing
February 2019

Dielectrophoresis-assisted creation of cell aggregates under flow conditions using planar electrodes.

Electrophoresis 2019 Feb 1. Epub 2019 Feb 1.

École Polytechnique Fédérale de Lausanne, EPFL-STI-IMT-LMIS4, Station 17, CH-1015, Lausanne, Switzerland.

We present a microfluidic platform allowing dielectrophoresis-assisted formation of cell aggregates of controlled size and composition under flow conditions. When specific experimental conditions are met, negative dielectrophoresis (nDEP) allows efficient concentration of cells towards electric field minima and subsequent aggregation. This bottom-up assembly strategy offers several advantages with respect to the targeted application: first, DEP offers precise control of spatial cell organization, which can be adjusted by optimizing electrode design. Read More

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http://dx.doi.org/10.1002/elps.201800435DOI Listing
February 2019

Multiplexing microelectrodes for dielectrophoretic manipulation and electrical impedance measurement of single particles and cells in a microfluidic device.

Electrophoresis 2019 Jan 31. Epub 2019 Jan 31.

Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda Genomic Resource Center, Soochow University, Suzhou, P. R. China.

This work presents a microfluidic device, which was patterned with (i) microstructures for hydrodynamic capture of single particles and cells, and (ii) multiplexing microelectrodes for selective release via negative dielectrophoretic (nDEP) forces and electrical impedance measurements of immobilized samples. Computational fluid dynamics (CFD) simulations were performed to investigate the fluidic profiles within the microchannels during the hydrodynamic capture of particles and evaluate the performance of single-cell immobilization. Results showed uniform distributions of velocities and pressure differences across all eight trapping sites. Read More

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http://dx.doi.org/10.1002/elps.201800433DOI Listing
January 2019
1 Read
3.028 Impact Factor

Understanding anomalous mobility of proteins on SDS-PAGE with special reference to the highly acidic extracellular domains of human E- and N-cadherins.

Electrophoresis 2019 Jan 31. Epub 2019 Jan 31.

Centre for Protein Science, Design and Engineering, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Mohali, Punjab, India.

During SDS-PAGE experiments, proteins generally display electrophoretic mobility in keeping with their molecular weights; however, some proteins display anomalies in mobility. Here, we focus attention on the anomalies displayed by the highly acidic ∼110 residues-long, sequence-homologous, structurally-analogous, extracellular domains of human E- and N-cadherin. We report that there is a strong correlation between the acidity of each domain and the degree of the anomaly that it displays. Read More

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http://dx.doi.org/10.1002/elps.201800219DOI Listing
January 2019

Electrophoresis of hydrophilic/hydrophobic rigid colloid with effects of relaxation and ion size.

Electrophoresis 2019 Jan 31. Epub 2019 Jan 31.

Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.

This article deals with a semi-analytical study on the electrophoresis of charged spherical rigid colloid by considering the effects of relaxation and ion size. The particle surface is taken to be either hydrophilic or hydrophobic in nature. In order to consider the ion size effect we have invoked the Carnahan and Starling model (J. Read More

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http://dx.doi.org/10.1002/elps.201800427DOI Listing
January 2019

Dielectrophoretic trapping of single leukemic cells using the conventional and compact optical measurement systems.

Electrophoresis 2019 Jan 30. Epub 2019 Jan 30.

Department of chemistry, Simon Fraser University, Burnaby, British Columbia, Canada.

Here, we report a microfluidic same-single-cell analysis to study the inhibition of multidrug resistance due to drug efflux on single leukemic cells. Drug efflux inhibition was investigated in the microfluidic chip using two different fluorescence detection systems, namely, a compact single-cell bioanalyzer and the conventional optical detection system constructed from an inverted microscope and a microphotometer. More importantly, a compact signal generator was used to conduct dielectrophoretic cell trapping together with the compact SCB. Read More

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http://dx.doi.org/10.1002/elps.201800451DOI Listing
January 2019
2 Reads

Detection of RNA-protein interactions using a highly sensitive non-radioactive electrophoretic mobility shift assay.

Electrophoresis 2019 Jan 30. Epub 2019 Jan 30.

Department of Biotechnology, Agricultural University of Athens, Athens, Greece.

Electrophoretic mobility shift assay (EMSA) is a sensitive technique useful in the identification and characterization of protein interactors with nucleic acids. This assay provides an efficient method to study DNA or RNA binding proteins and to identify nucleic acid substrates. The specific interaction plays important roles in many biological processes such as transcription, translation, splicing, and global gene expression. Read More

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http://doi.wiley.com/10.1002/elps.201800475
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http://dx.doi.org/10.1002/elps.201800475DOI Listing
January 2019
1 Read

Click regulation of cyclodextrin primary face for the preparation of novel chiral stationary phases.

Electrophoresis 2019 Jan 28. Epub 2019 Jan 28.

Tianjin Key Laboratory of Molecular Optoelectronic Science, Department of Chemistry, School of Science, Tianjin University, Tianjin, P. R. China.

In this study, a series of novel CD chiral stationary phases were fabricated by immobilization of mono-6 -deoxy-N -cyclodextrin onto silica surfaces followed by click regulation of CD primary face with 4-pentynoic acid (acidic moiety), 2-propynylamine (alkaline moiety) and L-propargylglycine (chiral amino acid moiety), respectively. Enantioseparations of various kinds of racemates including dansyl-amino acids, chiral lactides and diketones were conducted in reversed phase modes on these chiral stationary phases, where nearly forty diketones and chiral lactides were firstly separated on cyclodextrin stationary phases. 4-Pentynoic acid moiety can make the retention ability decline while amine moiety significantly enhanced the retention ability of the stationary phases. Read More

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http://dx.doi.org/10.1002/elps.201800418DOI Listing
January 2019
1 Read

Size-dependent electrophoretic migration and separation of water-soluble gold nanoclusters by capillary electrophoresis.

Electrophoresis 2019 Jan 25. Epub 2019 Jan 25.

Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Nanchong, P. R. China.

Recently, water-soluble gold nanoclusters (AuNCs) have attracted more and more attention due to their unique properties. In this study, penicillamine-protected gold nanoclusters (Pen-AuNCs) were synthesized and initially fractionated by sequential size-selective precipitation (SSSP). The crude Pen-AuNCs and SSSP fractions were separated by capillary zone electrophoresis (CZE) with a diode array detector. Read More

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http://dx.doi.org/10.1002/elps.201800347DOI Listing
January 2019
1 Read

Selective detection of cytochrome C by microchip electrophoresis based on an aptamer strategy.

Electrophoresis 2019 Jan 24. Epub 2019 Jan 24.

School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, P. R. China.

The release of cytochrome C (Cyt C) plays an important role in apoptosis. In this study, selective and sensitive detection of Cyt C based on an aptamer strategy coupled with MCE was developed. Following the binding of a specific aptamer to Cyt C, the aptamer exhibited an irregular state, reducing the binding affinity of a fluorescent probe, and thus preventing the aptamer-Cyt C complexes from detection within the MCE. Read More

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http://dx.doi.org/10.1002/elps.201800527DOI Listing
January 2019

Highlighting the uniqueness in dielectrophoretic enrichment of circulating tumor cells.

Electrophoresis 2019 Jan 24. Epub 2019 Jan 24.

Biomedical Institute for Global Health Research & Technology (BIGHEART), National University of Singapore, Singapore.

Circulating tumor cells (CTCs) play an essential role in the metastasis of tumors, and thus can serve as a valuable prognostic factor for malignant diseases. As a result, the ability to isolate and characterize CTCs is essential. This review underlines the potential of dielectrophoresis for CTCs enrichment. Read More

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http://dx.doi.org/10.1002/elps.201800446DOI Listing
January 2019

Insulator-based dielectrophoresis combined with the isomotive AC electric field and applied to single cell analysis.

Electrophoresis 2019 Jan 23. Epub 2019 Jan 23.

Department of Applied Physics, National Defense Academy, Kanagawa, Japan.

We developed an insulator-based dielectrophoretic (iDEP) creek-gap device that enables the isomotive movement of cells and that is suitable for determining their DEP properties. In the iDEP creek-gap device, a pair of planar insulators forming a single fan-shaped channel allows the induction of the isomotive iDEP force on cells. Hence, the cells' behavior is characterized by straight motion at constant velocity in the longitudinal direction of the channel. Read More

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http://dx.doi.org/10.1002/elps.201800380DOI Listing
January 2019
1 Read

Two-step signal amplification for high-sensitivity detection of biomarkers using gold nanoparticle-based conjugates.

Electrophoresis 2019 Jan 23. Epub 2019 Jan 23.

College of Chemistry, Research Center for Analytical Sciences, Institute of Polymer Chemistry, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin, P. R. China.

The measurement of biomarkers in bodily fluids is extremely important for diagnosing disease, monitoring disease progression, and evaluating treatment efficacy. In this paper, we present a highly sensitive and compatible gold nanoparticle (AuNP)-based, two-step signal amplification system for biomarker detection. First, AuNPs were coated onto the surfaces of 96-well plates to generate rough surfaces, which enable immobilization of many more capture antibodies than a smooth substrate. Read More

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http://dx.doi.org/10.1002/elps.201900007DOI Listing
January 2019
1 Read

The degree of substitution affects the enantioselectivity of sulfobutylether-β-cyclodextrin chiral stationary phases.

Electrophoresis 2019 Jan 22. Epub 2019 Jan 22.

Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Prague, Czech Republic.

Three chiral stationary phases were prepared by dynamic coating of sulfobutylether-β-cyclodextrin (SBE-β-CD) with different degrees of substitution, onto strong anion-exchange stationary phases. The enantioselective potential and stability of newly prepared chiral stationary phases were examined using a set of structurally different chiral analytes. Measurements were performed in RP-HPLC. Read More

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http://doi.wiley.com/10.1002/elps.201800471
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http://dx.doi.org/10.1002/elps.201800471DOI Listing
January 2019
4 Reads

Stiffness measurement of nanosized liposomes using solid-state nanopore sensor with automated recapturing platform.

Electrophoresis 2019 Jan 22. Epub 2019 Jan 22.

Department of Mechanical Engineering, Lyle School of Engineering, Southern Methodist University, Dallas, Texas, USA.

This paper describes a method to gauge the stiffness of nanosized liposomes - a nanoscale vesicle - using a custom-made recapture platform coupled to a solid-state nanopore sensor. The recapture platform electrically profiles a given liposome vesicle multiple times through automated reversal of the voltage polarity immediately following a translocation instance to re-translocate the same analyte through the nanopore - provides better statistical insight at the molecular level by analyzing the same particle multiple times compared to conventional nanopore platforms. The capture frequency depends on the applied voltage with lower voltages (i. Read More

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http://dx.doi.org/10.1002/elps.201800476DOI Listing
January 2019
1 Read

Investigation of the enantioselectivity of tetramethylammonium-lactobionate chiral ionic liquid based dual selector systems towards basic drugs in capillary electrophoresis.

Electrophoresis 2019 Jan 21. Epub 2019 Jan 21.

Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 210009, P. R. China.

Recently, chiral ionic liquids (CILs) have received increasing interest in chiral separation by capillary electrophoresis. Nevertheless, the lack of deep perception of the specific mechanism about CILs in CE for enantioseparation still perplexes a legion of researchers, despite the strenuous efforts. In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation. Read More

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http://dx.doi.org/10.1002/elps.201800422DOI Listing
January 2019
1 Read

A simple electrical approach to monitor dielectrophoretic focusing of particles flowing in a microchannel.

Electrophoresis 2019 Jan 20. Epub 2019 Jan 20.

Department of Civil Engineering and Computer Science, University of Rome Tor Vergata, Rome, Italy.

This paper reports an impedance-based system for the quantitative assessment of dielectrophoretic (DEP) focusing of single particles flowing in a microchannel. Particle lateral positions are detected in two electrical sensing zones placed before and after a DEP-focusing region, respectively. In each sensing zone, particle lateral positions are estimated using the unbalance between the opposite pulses of a differential current signal obtained with a straightforward coplanar electrode configuration. Read More

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http://dx.doi.org/10.1002/elps.201800423DOI Listing
January 2019
1 Read

Rapid and inexpensive method for the simple fabrication of PDMS-based electrochemical sensors for detection in microfluidic devices.

Electrophoresis 2019 Jan 18. Epub 2019 Jan 18.

Instituto de Química, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.

The fabrication of PDMS microfluidic structures through soft lithography is widely reported. While this well-established method gives high precision microstructures and has been successfully used for many researchers, it often requires sophisticated instrumentation and expensive materials such as clean room facilities and photoresists. Thus, we present here a simple protocol that allows the rapid molding of simple linear microchannels in PDMS substrates aiming microfluidics-based applications. Read More

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http://dx.doi.org/10.1002/elps.201800478DOI Listing
January 2019
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Immunobinding-induced alteration in the electrophoretic mobility of proteins: An approach to studying the preconcentration of an acidic protein under cationic isotachophoresis.

Electrophoresis 2019 Jan 17. Epub 2019 Jan 17.

Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA.

The objective of this study is to explore an approach for analyzing negatively charged proteins using paper-based cationic ITP. The rationale of electrophoretic focusing the target protein with negative charges under unfavorable cationic ITP condition is to modify the electrophoretic mobility of the target protein through antigen-antibody immunobinding. Cationic ITP was performed on a paper-based analytical device that was fabricated using fiberglass paper. Read More

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http://dx.doi.org/10.1002/elps.201800441DOI Listing
January 2019
1 Read
3.028 Impact Factor

The Next Forty Years of Electrophoresis.

Electrophoresis 2019 Jan;40(2):225-226

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http://dx.doi.org/10.1002/elps.201970015DOI Listing
January 2019
1 Read

The self-disproportionation of enantiomers (SDE): The effect of scaling down, potential problems versus prospective applications, possible new occurrences, and unrealized opportunities?

Electrophoresis 2019 Jan 17. Epub 2019 Jan 17.

Molecular Structure Analysis, German Cancer Research Center (DKFZ), Heidelberg, Germany.

This commentary discusses an important, though not widely appreciated, chiral phenomenon of molecular chirality that effectively always occurs whenever nonracemic samples are subjected to practically any physicochemical process (e.g., force field, recrystallization, sublimation, even distillation, etc. Read More

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http://doi.wiley.com/10.1002/elps.201800414
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http://dx.doi.org/10.1002/elps.201800414DOI Listing
January 2019
4 Reads

Quantification of controlled release leuprolide and triptorelin in rabbit plasma using electromembrane extraction coupled with HPLC-UV.

Electrophoresis 2019 Jan 17. Epub 2019 Jan 17.

Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

An electromembrane extraction followed by HPLC-UV technique was developed and validated for quantification of leuprolide and triptorelin in rabbit plasma. The influencing parameters on the extraction efficiency were optimized using experimental design methodology. The optimized conditions were found to be; supported liquid membrane: a mixture of 1-octanol and 2-ethyl hexanol (1:1) containing 10% v/v di(2-ethylhexyl) phosphate, applied voltage: 5 V, extraction time: 5 min, pH of the donor phase: 4. Read More

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http://dx.doi.org/10.1002/elps.201800481DOI Listing
January 2019
1 Read

Determination of formaldehyde in single cell by capillary electrophoresis with LIF detection.

Electrophoresis 2019 Jan 17. Epub 2019 Jan 17.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, P. R. China.

As a small molecule gas, formaldehyde (FA) is the simplest carbonyl active material and plays an important role in the carbon cycle of metabolism. However, due to the volatile nature of the gas, it is difficult to accurately quantify its content, which limits the study of the mechanism of action in life activities. Thus, an efficient approach to quantitative detection of FA in cells especially in single cell is urgent needed. Read More

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http://dx.doi.org/10.1002/elps.201800399DOI Listing
January 2019
1 Read

Influence of weak groups on polyelectrolyte mobilities.

Electrophoresis 2019 Jan 15. Epub 2019 Jan 15.

Institute for Computational Physics, Universität Stuttgart, Stuttgart, Germany.

The ionization of dissociable groups in weak polyelectrolytes does not occur in a homogenous fashion. Monomer connectivity imposes constraints on the localization of the dissociated (charged) monomers that affect the local electric potential. As a result, the mean bare charge along a weak polyelectrolyte can vary depending on the proximity to topological features (e. Read More

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http://dx.doi.org/10.1002/elps.201800346DOI Listing
January 2019
1 Read

Experimental design and measurement uncertainty in ligand binding studies by affinity capillary electrophoresis.

Electrophoresis 2019 Jan 14. Epub 2019 Jan 14.

Technische Universität Braunschweig, Institute of Medicinal and Pharmaceutical Chemistry, Braunschweig, Germany.

In all life sciences ligand binding assays (LBAs) play a crucial role. Unfortunately these are very error prone. One part of this uncertainty results from the unavoidable random measurement uncertainty, another part can be attributed to the experimental design. Read More

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http://dx.doi.org/10.1002/elps.201800450DOI Listing
January 2019
1 Read

On-line sample preconcentration by polarity switching in floating electrode-integrated microchannel.

Electrophoresis 2019 Jan 13. Epub 2019 Jan 13.

Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan.

In this study, we found that the polarity switching was effective to enrich and separate fluorescent analytes which have weakly-dissociated groups in a floating platinum electrode (width, 50 μm; thickness, 2.5 μm)-integrated straight-channel in microchip electrophoresis (MCE). In the straight channel filled with an Alexa Flour 488 (AF488) solution, a sharp peak was observed after the polarity inversion with a 530-fold enhancement of the sensitivity relative to the conventional MCE analysis. Read More

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http://doi.wiley.com/10.1002/elps.201800501
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http://dx.doi.org/10.1002/elps.201800501DOI Listing
January 2019
6 Reads

Comparison of two different electrophoretic methods in studying the genetic diversity among plantains (Musa spp.) using ISSR markers.

Electrophoresis 2019 Jan 10. Epub 2019 Jan 10.

ICAR-National Research Centre for Banana, Tiruchirappalli, Tamil Nadu, India.

Inter simple sequence repeat markers were employed for the genotyping of 16 plantain ecotypes. Two different electrophoretic systems namely conventional gel electrophoresis (CVGE) and fully automated high-resolution CGE were used to evaluate the genetic diversity. Comparative analysis indicated that all parameters related to marker informativeness were higher in CGE except polymorphic information content. Read More

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http://dx.doi.org/10.1002/elps.201800456DOI Listing
January 2019
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Impedance-based viscoelastic flow cytometry.

Electrophoresis 2019 Jan 10. Epub 2019 Jan 10.

UNAM-National Nanotechnology Research Center and Institute of Materials Science and Nanotechnology, Bilkent University, Ankara, Turkey.

Elastic nature of the viscoelastic fluids induces lateral migration of particles into a single streamline and can be used by microfluidic based flow cytometry devices. In this study, we investigated focusing efficiency of polyethylene oxide based viscoelastic solutions at varying ionic concentration to demonstrate their use in impedimetric particle characterization systems. Rheological properties of the viscoelastic fluid and particle focusing performance are not affected by ionic concentration. Read More

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http://dx.doi.org/10.1002/elps.201800365DOI Listing
January 2019
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A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.

Electrophoresis 2019 Jan 10. Epub 2019 Jan 10.

APL Research Centre-II (A Division of Aurobindo Pharma Ltd), Hyderabad, Telangana, India.

A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance. Enantiomeric separation was achieved on a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase. Mobile phase consists of aqueous diammonium hydrogen phosphate buffer and acetonitrile in the ratio of 35:65 v/v. Read More

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http://doi.wiley.com/10.1002/elps.201800502
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http://dx.doi.org/10.1002/elps.201800502DOI Listing
January 2019
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A smart and portable micropump for stable liquid delivery.

Electrophoresis 2019 Jan 9. Epub 2019 Jan 9.

Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, P. R. China.

Precise and reliable liquid delivery is vital for microfluidic applications. Here, we illustrate the design, fabrication, characterization, and application of a portable, low cost, and robust micropump, which brings solution to stable liquid delivery in microfluidic environment. The pump is designed with three optional speeds of different pumping flow rates, and it can be simply actuated by spring-driven mechanism. Read More

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http://doi.wiley.com/10.1002/elps.201800494
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http://dx.doi.org/10.1002/elps.201800494DOI Listing
January 2019
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Scaling behavior in on-chip field-amplified sample stacking.

Electrophoresis 2019 Jan 9. Epub 2019 Jan 9.

Department of Mechanical Engineering, Indian Institute of Technology Delhi, Delhi, India.

Field amplified sample stacking (FASS) uses differential electrophoretic velocity of analyte ions in the high-conductivity background electrolyte zone and low conductivity sample zone for increasing the analyte concentration. The stacking rate of analyte ions in FASS is limited by molecular diffusion and convective dispersion due to nonuniform electroosmotic flow (EOF). We present a theoretical scaling analysis of stacking dynamics in FASS and its validation with a large set of on-chip sample stacking experiments and numerical simulations. Read More

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http://dx.doi.org/10.1002/elps.201800392DOI Listing
January 2019
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Peptide nucleic acid and amino acid modified peptide nucleic acid analysis by capillary zone electrophoresis.

Electrophoresis 2019 Jan 7. Epub 2019 Jan 7.

School of Life Science, Beijing Institute of Technology, Beijing, P. R. China.

A rapid, high resolution, and low sample consumption CZE method is developed for peptide nucleic acid (PNA) analysis for the first time. 30% v/v acetonitrile in PNA sample and 20% v/v acetonitrile in 50 mM borax-boric acid (pH 8.7) as BGE were employed after optimization. Read More

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http://doi.wiley.com/10.1002/elps.201800312
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http://dx.doi.org/10.1002/elps.201800312DOI Listing
January 2019
3 Reads
3.028 Impact Factor

50, 100, 1000 Years: Happy Anniversary Electrophoresis!

Electrophoresis 2019 Jan;40(1):11-15

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http://dx.doi.org/10.1002/elps.201800447DOI Listing
January 2019
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Reviews 2019.

Authors:
Ziad El Rassi

Electrophoresis 2019 Jan;40(1):16

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http://dx.doi.org/10.1002/elps.201870197DOI Listing
January 2019
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