602,462 results match your criteria Electrophoresis [Journal]
J Plant Physiol 2018 Nov 20;232:94-99. Epub 2018 Nov 20.
Institute of Basic Biological Problems of Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
The carrier of carbonic anhydrase (CA) activity was detected in gel among low molecular mass proteins from pea, spinach and Arabidopsis, after nondenaturing electrophoresis in PAAG of the dodecyl-β-d-maltoside treated PSII membranes (the fragments of thylakoid membrane containing PSII complexes). The elimination of Mn-stabilizing protein PsbO by treatment of PSII membranes with salts, did not lead to a decrease in CA activity observed in the gel although it reduced the amount of this protein down to 25% compared to the original sample. The isolated protein PsbO did not demonstrated CA activity. Read More
J Plant Physiol 2018 Nov 23;232:171-179. Epub 2018 Nov 23.
Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, Japan; Research Institute of Chuo University, Kasuga, Bunkyo, Tokyo 112-8551, Japan. Electronic address:
Oxygen availability in water is considered one of the most important factors for growth and productivity in aquatic submerged macrophytes. In the present study, the growth, stress responses, and metabolic changes in Potamogeton anguillanus Koidz. (Potamogetonaceae) were assessed after a 21-day exposure to low (hypoxia; dissolved oxygen, DO < 1 mg/L) or null (anoxia) oxygen concentrations in water. Read More
Int J Lab Hematol 2018 Dec 8. Epub 2018 Dec 8.
Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium.
Introduction: T-cell receptor gene (TRG) rearrangement profiling is an essential component of the workup at diagnosis of T-cell malignancies. TRG amplification by polymerase chain reaction (PCR) and analysis by capillary electrophoresis (PCR-CE) is mostly widely used but is hampered by a subjective interpretation of its results and possible false-positive interpretation of clonality. Several studies evaluated the advantage of TRG rearrangement analysis by Next Generation Sequencing (TRG-NGS), however few have proposed an adequate data interpretation algorithm. Read More
J Cell Physiol 2018 Dec 8. Epub 2018 Dec 8.
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
Irradiation (IR) can be used to treat cancer by inducing complex and irreparable DNA damage in the cancer cells, which may lead to their apoptotic death. However, little is known about the molecular mechanism of this DNA damage. Here, the non-small-cell lung cancer cell line A549 was treated with either X-ray or carbon ion combined with bleomycin (BLM). Read More
Curr Med Sci 2018 Dec 7;38(6):1096-1102. Epub 2018 Dec 7.
Department of Obstetrics and Gynecology, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China.
Biodistribution and toxicity assessment are critical for safe clinical use of newly developed medicines. Superparamagnetic iron oxide nanoparticles (SPION) are effective carriers for targeted drug delivery. This study aimed to examine the toxicity and biodistribution of SPION coated with polyethylenimine (PEI) (SPION-PEI) designed for small interfering RNA (siRNA) delivery both in vitro and in vivo. Read More
Trop Anim Health Prod 2018 Dec 10. Epub 2018 Dec 10.
Department of Basic Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Pasteurella multocida (P. multocida) is an important pathogen that causes bovine respiratory disease (BRD) in China and other countries. To investigate the antimicrobial susceptibility of P. Read More
Anal Bioanal Chem 2018 Dec 10. Epub 2018 Dec 10.
Faculty of Chemistry, Aalen University, 73430, Aalen, Germany.
Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples. Protein glycosylation is one of the most common post-translational modifications associated with a wide range of biological functions and human diseases. Due to their high structural variability, the analysis of glycans still represents a challenging task. Read More
Radiat Prot Dosimetry 2018 Dec 11. Epub 2018 Dec 11.
Department of Radiation Dosimetry, Nuclear Physics Institute of the Czech Academy of Sciences, Na Truhlárce 39/64, Prague, Czech Republic.
A compromised detection of radiation-induced plasmid DNA fragments results in underestimation of calculated damage yields. Electrophoretic methods are easy and cheap, but they can only detect a part of the fragments, neglecting the shortest ones. These can be detected with atomic force microscopy, but at the expense of time and price. Read More
Soft Matter 2018 Dec 11. Epub 2018 Dec 11.
Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, New Jersey 08544, USA.
We explore the consequences of micelle formation for diffusiophoresis of charged colloidal particles in ionic surfactant concentration gradients, using a quasi-chemical association model for surfactant self assembly. The electrophoretic contribution to diffusiophoresis is determined by re-arranging the Nernst-Planck equations, and the chemiphoretic contribution is estimated by making plausible approximations for the density profiles in the electrical double layer surrounding the particle. For sub-micellar solutions we find that a particle will typically be propelled down the concentration gradient, although electrophoresis and chemiphoresis are finely balanced and the effect is sensitive to the detailed parameter choices and simplifying assumptions in the model. Read More
New Microbes New Infect 2019 Jan 16;27:29-35. Epub 2018 Nov 16.
Department of Pathobiology, School of Public Health and Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Health-care workers may serve as a reservoir for dissemination of methicillin-resistant (MRSA) to patients in hospital settings. The present study aimed to screen MRSA in nasal swabs of health-care workers and clinical specimens from patients and investigate the possible relationship between these isolates at a university hospital in Tehran, Iran. Additionally, we aimed to identify potential risk factors for MRSA colonization in health-care workers. Read More
J Venom Anim Toxins Incl Trop Dis 2018 4;24:37. Epub 2018 Dec 4.
1Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (FCFRP-USP), Avenida do Café, s/n, B. Monte Alegre, Ribeirão Preto, SP CEP 14040-903 Brazil.
Background: L-amino acid oxidases isolated from snake venoms (SV-LAAOs) are enzymes that have great therapeutic potential and are currently being investigated as tools for developing new strategies to treat various diseases, including cancer and bacterial infections. The main objective of this study was to make a brief evaluation of the enzymatic stability of two LAAOs, one isolated from (BjussuLAAO-II) and the other from (BmooLAAO-I) venoms.
Methods And Results: The enzymatic activity and stability of both LAAOs were evaluated by microplate colorimetric assays, for which BjussuLAAO-II and BmooLAAO-I were incubated with different L-amino acid substrates, in the presence of different ions, and at different pH ranges and temperatures. Read More
Front Microbiol 2018 26;9:2798. Epub 2018 Nov 26.
College of Marine Sciences, South China Agricultural University, Guangzhou, China.
The ubiquitin-proteasome system (UPS) serves as the major intracellular pathway for protein degradation and plays crucial roles in several cellular processes. However, little is known about the potential actions of the UPS during fish virus infection. In this study, we elucidated the possible roles of UPS in the life cycle of Singapore grouper iridovirus (SGIV); a large DNA virus that usually causes serious systemic diseases with high mortality in groupers. Read More
PLoS One 2018 11;13(12):e0198717. Epub 2018 Dec 11.
Laboratory of Genetically Encoded Small Molecules, The Rockefeller University, New York, NY, United States of America.
Here we describe GoFish, a strategy for single-species environmental DNA (eDNA) presence/absence assays using nested PCR. The assays amplify a mitochondrial 12S rDNA segment with vertebrate metabarcoding primers, followed by nested PCR with M13-tailed, species-specific primers. Sanger sequencing confirms positives detected by gel electrophoresis. Read More
Clin Chem Lab Med 2018 Dec 8. Epub 2018 Dec 8.
Department of Clinical Chemistry and Diagnostic Endocrinology, Mater Misericordiae University Hospital, Dublin, Ireland.
Sci Total Environ 2018 Dec 3;657:7-15. Epub 2018 Dec 3.
Université Clermont Auvergne, CNRS, Laboratoire "Microorganismes: Génome et Environnement", F-63000 Clermont-Ferrand, France; Service d'hygiène hospitalière, CHU de Clermont-Ferrand, Clermont-Ferrand, France. Electronic address:
Bacterial resistance to carbapenem, which is mainly due to the successful dissemination of carbapenemase-encoding genes, has become a major health problem. Few studies have aimed to characterize the level of resistance in the environment, notably in hospital wastewater, which is a likely hotspot for exchange of antibiotic resistance genes. In this work, we looked for the presence of imipenem-resistant bacteria and imipenem in the effluent of the teaching hospital of Clermont-Ferrand, France. Read More
Ecotoxicol Environ Saf 2018 Dec 7;170:293-299. Epub 2018 Dec 7.
Laboratory of Biotechnology, Research Centre for Biological Sciences, Universidad Autónoma de Tlaxcala, Ixtacuixtla C.P. 90062, Tlaxcala, Mexico. Electronic address:
Di(2-ethylhexyl) phthalate (DEHP) is a widely used plasticizer, which is considered an endocrine disrupting pollutant. Growth kinetics and esterases activity by biochemical tests and polyacrylamide gel electrophoresis were characterized for Fusarium culmorum grown in DEHP-supplemented (1000 mg/L) medium as the only carbon source and in control medium with glucose. Intermediate compounds of biodegraded DEHP were identified by GC-MS. Read More
Theriogenology 2018 Nov 28;126:41-48. Epub 2018 Nov 28.
Department of Production Animal Health, University of Calgary, Faculty of Veterinary Medicine, Calgary, AB, Canada.
Sperm are highly specialized compartmentalized cells, with unique compositional, morphological and functional properties, including a plasma membrane that undergoes dynamic protein remodeling and surface modifications. Seminal plasma is a highly complex biological fluid containing proteins, amino acids, enzymes, fructose and other carbohydrates, lipids, major minerals and trace elements. Seminal plasma proteins are involved in regulation of osmotic pressure and pH of seminal plasma, transport of ions, lipid and hormones. Read More
J Pharm Biomed Anal 2018 Dec 1;165:129-134. Epub 2018 Dec 1.
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China. Electronic address:
Proteolytic digestion and peptide separation are key steps in proteomic study, which has attracted much attention. A novel immobilized trypsin microreactor based on monolithic capillary column was developed for rapid proteolytic digestion in this work. The monolithic support was prepared using itaconic acid as functional monomer within a silanized fused-silica capillary. Read More
J Proteomics 2018 Dec 6. Epub 2018 Dec 6.
Spectrometry, Sample Preparation and Mechanization Group - GEPAM, Institute of Chemistry, University of Campinas - UNICAMP, Campinas, Brazil; National Institute of Science and Technology for Bioanalytics, Brazil. Electronic address:
A combination between gel electrophoresis and smartphone technology is applied for quantifying proteins in a serum sample. Electrophoresis not only allows one to separate different proteins but also to build-up a calibration curve for those proteins. To expand its applicability, a smartphone allows one to capture a gel image, through its camera. Read More
J Hosp Infect 2018 Dec 6. Epub 2018 Dec 6.
Department of Nosocomial Infection Management and Disease Control, Chinese PLA General Hospital, Beijing, China. Electronic address:
Background: Following the alarming outbreak of carbapenem-resistant Klebsiella pneumonia (CRKP) in five intensive care units (ICUs) of a tertiary care hospital in China, a prospective investigation of CRKP colonized/infected patients was conducted.
Aim: To describe the diffusion and transmission of CRKP among epidemiologically linked ICU patients, staff and environment.
Methods: Enhanced CRKP infected/colonized case monitoring was performed by the real-time nosocomial infection surveillance system (RT-NISS). Read More
Anal Biochem 2018 Dec 4. Epub 2018 Dec 4.
Department of Chemistry, Moscow State University, Moscow, 119991, Russia.
A method is described for the direct detection of unstable cysteine peptidase activity in polyacrylamide gels after native electrophoresis using new selective fluorogenic peptide substrates, pyroglutamyl-phenylalanyl-alanyl-4-amino-7-methylcoumaride (Glp-Phe-Ala-AMC) and pyroglutamyl-phenylalanyl-alanyl-4-amino-7-trifluoromethyl-coumaride (Glp-Phe-Ala-AFC). The detection limit of the model enzyme papain was 17 pmol (0.29 μg) for Glp-Phe-Ala-AMC and 43 pmol (0. Read More
Arch Biochem Biophys 2018 Dec 5. Epub 2018 Dec 5.
Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya, 484-8603, Japan; Plasma Medical Science Global Innovation Center, Nagoya University, Furo-cho, Chikusa, Nagoya, 484-8601, Japan; Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa, Nagoya, 484-8601, Japan.
Non-equilibrium atmospheric pressure plasma (NEAPP) is a mixture of radicals, electrons, anions, cations and light at near body temperature. Plasma-activated medium (PAM) is realized using NEAPP provided by engineered devices and irradiated to a cell culture medium for a period of 600 s. Glioblastoma cells U251SP cultivated in PAM previously indicated that antitumor effects induced PAM-specific apoptotic cell-death. Read More
J Environ Sci (China) 2019 Feb 17;76:121-132. Epub 2018 Apr 17.
State Key Laboratory of Urban Water Resource and Environment (SKLUWRE), School of Environment, Harbin Institute of Technology, Harbin 150090, China.
It was focused on the effect of different sludge concentrations on the performances of an algal-activated sludge symbiotic system in terms of wastewater treatment, algal-activated sludge characteristics and community structure. The results showed that the highest nutrient removal efficiencies were obtained in the reactor R2 with soluble chemical oxygen demand (sCOD), ammonia nitrogen (NH-N) and phosphate (PO -P) removal efficiencies of (90.6±2. Read More
Am J Infect Control 2018 Dec 5. Epub 2018 Dec 5.
Department of Infectious Disease and Clinical Microbiology, Ege University Faculty of Medicine, Izmir, Turkey.
We presented a sepsis outbreak caused by Serratia marcescens from contaminated propofol to raise awareness. Three patients had sepsis syndrome after chest surgery. Isolation of S marcescens from patients' respiratory and blood samples alerted us to a possible outbreak. Read More
BMC Microbiol 2018 Dec 7;18(1):207. Epub 2018 Dec 7.
Key laboratory of pest management of horticultural crop of Hunan province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, No 726 Second Yuanda Road, Furong District, Changsha, 410125, Hunan province, People's Republic of China.
Background: Pyrazosulfuron-ethyl is a long lasting herbicide in the agro-ecosystem and its residue is toxic to crops and other non-target organisms. A better understanding of molecular basis in pyrazosulfuron-ethyl tolerant organisms will shed light on the adaptive mechanisms to this herbicide.
Results: Pyrazosulfuron-ethyl inhibited biomass production in Rhodopseudomonas palustris PSB-S, altered cell morphology, suppressed flagella formation, and reduced pigment biosynthesis through significant suppression of carotenoids biosynthesis. Read More
J Agric Food Chem 2018 Dec 10. Epub 2018 Dec 10.
Di-n-butyl phthalate (DBP) as an endocrine disrupting chemical that tends to be accumulated in crops poses great risks to human health through the food chain. To identify the molecular mechanism underlying differences in their DBP accumulation, the root physiological and proteomic responses to DBP stress of two Brassica parachinensis cultivars, a high-DBP accumulator (Huaguan) and a low-DBP accumulator (Lvbao), were investigated. Root damage of greater severity and significantly greater (p<0. Read More
Anal Chem 2018 Dec 10. Epub 2018 Dec 10.
The performance of photon-upconversion nanoparticles (UCNPs) as background-free luminescent labels in bioanalytical applications strongly depends on the preparation of well-defined and water-dispersible nanoconjugates. We have exploited the separation power of agarose gel electrophoresis to purify milligram amounts of homogeneous UCNPs covered with carboxylated silica, biotin or streptavidin with recovery rates of 30 to 50%. Clusters containing discrete numbers of UCNPs were isolated from the gel and reanalyzed by agarose gel electrophoresis, single nanoparticle upconversion microscopy and additional complementary methods. Read More
Anal Chem 2018 Dec 9. Epub 2018 Dec 9.
Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine that bisects the mannose core. Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contained varying levels of α2-6 linked sialic acid, galactose, and bisected N-acetylglucosamine. Monitoring the relative abundance of bisecting N-acetylglucosamine is relevant to physiological processes. Read More
ACS Infect Dis 2018 Dec 10. Epub 2018 Dec 10.
Hepatitis B virus (HBV) core protein is a small protein with 183 amino acid residues and assembles the pre-genomic (pg) RNA and viral DNA polymerase to form nucleocapsids. During the last decades, several groups have reported HBV core protein allosteric modulators (CpAMs) with distinct chemical structures. CpAMs bind to the hydrophobic HAP pocket located at the dimer-dimer interface and induce allosteric conformational changes in the core protein subunits. Read More
Cell Signal 2018 Dec 3. Epub 2018 Dec 3.
Division of Cell Matrix Biology & Regenerative Medicine, UK. Electronic address:
Photodamage in chronically sun-exposed skin manifests clinically as deep wrinkles and histologically as extensive remodelling of the dermal extracellular matrix (ECM) and in particular, the elastic fibre system. We have shown previously that loss of fibrillin microfibrils, a key elastic fibre component, is a hallmark of early photodamage and that these ECM assemblies are susceptible in vitro to physiologically attainable doses of ultraviolet radiation (UVR). Here, we test the hypotheses that UVR-mediated photo-oxidation is the primary driver of fibrillin microfibril and fibronectin degradation and that prior UVR exposure will enhance the subsequent proteolytic activity of UVR-upregulated matrix metalloproteinases (MMPs). Read More
PLoS One 2018 6;13(12):e0208468. Epub 2018 Dec 6.
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand.
Conjugation is a type of horizontal gene transfer (HGT) that serves as the primary mechanism responsible for accelerating the spread of antibiotic resistance genes in Gram-negative bacteria. The present study aimed to elucidate the mechanisms underlying the conjugation-mediated gene transfer from the extensively drug-resistant Acinetobacter baumannii (XDR-AB) and New Delhi Metallo-beta-lactamase-1-producing Acinetobacter baumannii (NDM-AB) to environmental isolates of Acinetobacter spp. Conjugation experiments demonstrated that resistance to ticarcillin and kanamycin could be transferred from four donors to two sodium azide-resistant A. Read More
J Dairy Res 2018 Dec 6:1-6. Epub 2018 Dec 6.
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos,Universidade de São Paulo,Sao Paulo,Brazil.
The bovine whey consists of more than 200 different types of proteins, of which β-lactoglobulin, α-lactalbumin, serum albumin, immunoglobulins and lactoferrin predominate. However, their concentrations are not stable due to the existence of protein dynamics during a transition from colostrum secretion to mature milk. To evaluate the dynamics of whey proteins of Jersey cows during a colostral phase and first month of lactation and an influence of the number of lactations, 268 milk samples from 135 Jersey cows were selected through a clinical evaluation. Read More
J Dairy Res 2018 Dec 6:1-6. Epub 2018 Dec 6.
Department of Food Science,Aarhus University, AU Foulum,Blichers Alle 20, DK-8830 Tjele,Denmark.
The process of agglutination causes firm cream layers in bovine milk, and a functioning agglutination mechanism is paramount to the quality of non-homogenized milks. The phenomenon is not well-described, but it is believed to occur due to interactions between immunoglobulins (Ig) and milk fat globules. For the first time, this paper demonstrates how the process of agglutination can be visualized using confocal laser scanning microscopy, rhodamine red and a fluoresceinisothiocynat-conjugated immunoglobulin M antibody. Read More
J Appl Microbiol 2018 Dec 5. Epub 2018 Dec 5.
Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Aims: The aim of this study was to evaluate the elimination of patulin by Lactobacillus plantarum ATCC 8014 from artificially contaminated apple juice and its dependence on prebiotic, citric acid, and ascorbic acid content.
Methods And Results: A central composite design was used for studying each of the three factors at five levels to find the optimum concentrations. The results showed that inserting 2. Read More
J Cell Physiol 2018 Dec 5. Epub 2018 Dec 5.
Department of Molecular Medicine, "Sapienza" University of Rome, Rome, Italy.
Lung cancer (LC) is the most common type of cancer and the second cause of death worldwide in men and women after cardiovascular diseases. Non-small-cell lung cancer (NSCLC) is the most frequent type of LC occurring in 85% of cases. Developing new methods for early detection of NSCLC could substantially increase the chances of survival and, therefore, is an urgent task for current research. Read More
Anal Bioanal Chem 2018 Dec 6. Epub 2018 Dec 6.
Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
Capillary electrophoresis (CE) is excellent at separating all the ions in a sample but is rarely used as a result of its detection issue and easy loss of very fast ions by common one-end injection methods. Herein we propose a newly developed method aimed at simultaneous determination of positive and negative ions with a home-made CE device, featuring bi-end injection and contactless conductivity detection at the middle. By simply using 2. Read More
Methods Mol Biol 2019 ;1888:127-139
Chemical Biology Research Group, RIKEN CSRS, Wako, Saitama, Japan.
Recent improvements in technologies such as omics analysis have enabled us to acquire a large amount of data regarding the biological changes in cells treated with bioactive small molecules. Using such data, a variety of profiling methods have been established for target identification of such bioactive compounds. In this chapter, we describe a proteomic profiling system, ChemProteoBase, based on proteome analysis using two-dimensional difference gel electrophoresis. Read More
Dig Dis Sci 2018 Dec 5. Epub 2018 Dec 5.
Department of Oncology Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
Background: Circular RNAs (circRNAs), a special class of noncoding RNAs with the characteristic of covalent closed-loop structure, have been widely found in various organisms. Growing evidence has shown that circRNAs play a crucial role in regulating biological functions of cancers. However, the specific role of circRNAs in esophageal squamous cell carcinoma (ESCC) remains largely unknown. Read More
Data Brief 2018 Dec 14;21:1944-1949. Epub 2018 Nov 14.
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), A.P. 70228, Ciudad Universitaria, Ciudad de México 04510, México.
Carbohydrate-binding modules (CBMs) are auxiliary domains into glycoside-hydrolases that allow the interaction between the insoluble substrate and the solubilized enzyme, through hydrophobic, CH-π interactions and hydrogen bonds. Here, we present the data article related to the interaction of one CBM26 and some mutated proteins with soluble α-glucans determined by enzyme-linked carbohydrate-binding assay, isothermal titration calorimetry (ITC), and affinity gel electrophoresis (AGE). The data of the behavior of proteins in presence and absence of substrate analyzed by circular dichroism CD and thermofluor are also presented. Read More
J Cancer 2018 20;9(22):4234-4241. Epub 2018 Oct 20.
Institute of Pathology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany.
Squamous cell carcinoma of the penis is a rare but often aggressive disease. A large proportion of penile cancers are associated with HPV infection, mainly with HPV high-risk subtypes 16 and 18. From other HPV-related malignancies a link between a functional SNP in the p53 gene (rs1042522, p. Read More
Infect Drug Resist 2018 8;11:2211-2217. Epub 2018 Nov 8.
Department of Microbiology, Faculty of Dentistry and Oral Medicine, Future University, Cairo, Egypt.
Introduction: is a significant hospital-acquired pathogen, and many outbreaks of infection have been reported in neonates. We report a sudden breakout of harboring the and metallo-β-lactamase (MBL) genes that occurred from March to August 2015 in the neonatal intensive care unit of Cairo University Hospital, Cairo, Egypt.
Methods: During the study period, 40 nonduplicate clinical isolates of were collected from blood culture samples. Read More
Drug Des Devel Ther 2018 13;12:3899-3912. Epub 2018 Nov 13.
Department of Genetics and Cell Biology, Basic Medical College, Qingdao University, Qingdao 266071, People's Republic of China,
Background: To improve the targeting ability of antitumor drugs, we identified the antigens with high expression on the surface of tumor cells associated with tumor escape, such as the complement regulatory protein CD55 molecule, which is also known as the decay accelerating factor. In this study, phage display technology was used to screen and identify CD55-specific ligand peptide (CD55sp) bound to CD55 molecule on the surface of cervical cancer HeLa cells. We then explored the role of this peptide in inhibiting the growth of cervical cancer cells in vitro. Read More
Nat Protoc 2018 Dec 5. Epub 2018 Dec 5.
Departments of Chemistry and of Molecular Biosciences, Northwestern University, Evanston, IL, USA.
Top-down proteomics (TDP) by mass spectrometry (MS) is a technique by which intact proteins are analyzed. It has become increasingly popDesalting and concentrating GELFrEEular in translational research because of the value of characterizing distinct proteoforms of intact proteins. Compared to bottom-up proteomics (BUP) strategies, which measure digested peptide mixtures, TDP provides highly specific molecular information that avoids the bioinformatic challenge of protein inference. Read More
Sci Rep 2018 Dec 5;8(1):17675. Epub 2018 Dec 5.
State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, MOE-LSB and MOE-LSC, Department of Bioinformatics and Biostatistics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.
Measurement of cell-free fetal DNA (cffDNA) is an indispensable process for non-invasive prenatal screening (NIPS). According to recent studies, cffDNA in maternal plasma can be enriched for various lengths of fragments, and a sufficient amount of cffDNA can effectively eliminate background interference on the part of maternal DNA. Therefore, we developed a simple and effective separation method, improved NIPS (iNIPS), that enriches the fetal fraction and improves the accuracy of NIPS for fetal aneuploid detection. Read More
BMC Cardiovasc Disord 2018 Dec 5;18(1):224. Epub 2018 Dec 5.
Department of Cardiology, Nihon University Hospital, 1-6 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-8309, Japan.
Background: Lecithin-cholesterol acyltransferase (LCAT) is believed to be involved in reverse cholesterol transport, which is known to play a key role in suppression of atherosclerosis. However, recent investigations have demonstrated that higher LCAT activity, measured in terms of the serum cholesterol esterification rate by an endogenous substrate method, is associated with increased formation of triglyceride (TG)-rich lipoproteins (TRLs), leading to a decrease in the low-density lipoprotein (LDL) particle size. The purpose of this hospital-based longitudinal study was to clarify the causal relationship between changes in the LCAT activity and changes in the LDL-particle size. Read More
Foods 2018 Dec 4;7(12). Epub 2018 Dec 4.
Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia.
In this study, the presence of was assessed along the production process of fermented sausages in a small-scale facility. Following the isolation of the pathogen from the final product (ISO 11290-1), retrospective sampling was performed during the production of a new batch of sausages, including raw materials, casings, additives, sausage mixtures, sausages during fermentation, and environmental samples. was recovered from the following sampling points: the defrosting room and the cuttering, mixing, stuffing, and fermentation phases. Read More
Protein Expr Purif 2018 Dec 1;155:86-94. Epub 2018 Dec 1.
Department of Biochemistry and Molecular Biology, MS 200, University of Nevada, Reno, NV, 89557, USA.
This paper presents a simultaneous isolation of pure, intact chloroplasts and mitochondria from mature leaves of Ice plant (Mesembryanthemum crystallinum) and mitochondrial protein preparation for two-dimensional electrophoresis (2DE) analysis under well watered and water -deficit stressed treatments. The washed chloroplasts and mitochondria were purified with Percoll gradients prepared using a Master flex R pump. The chloroplast and mitochondrial proteins were extracted in lysis buffer containing a protease inhibitor mix supplemented with 1 μM Leupeptin and 1 μM E64, followed by precipitation with ice-cold acetone. Read More
J Proteome Res 2018 Dec 3. Epub 2018 Dec 3.
Cystic fibrosis (CF) is a complex multi-organ disorder that is among the most common fatal genetic diseases benefiting from therapeutic interventions early in life. Newborn screening (NBS) for pre-symptomatic detection of CF currently relies on a two-stage immunoreactive trypsinogen (IRT) and cystic fibrosis transmembrane conductance regulator (CFTR) mutation panel algorithm that is sensitive, but not specific for identifying affected neonates with a low positive predictive value. For the first time, we report the discovery of a panel of CF-specific metabolites from a single 3. Read More
J Cell Biochem 2018 Dec 2. Epub 2018 Dec 2.
Department of Studies in Biochemistry, University of Mysore, Manasagangothri, Mysuru, India.
Echis carinatus envenomation leads to severe tissue necrosis at the bitten site by releasing DNA from immune cells that blocks the blood flow. An earlier report has shown that exogenous DNase 1 offers protection against such severe local tissue necrosis. Tricosanthus tricuspidata is a medicinal plant and the paste prepared from its leaves has been used extensively for the treatment of snakebite-induced tissue necrosis. Read More