5,981 results match your criteria Diagnostic microbiology and infectious disease[Journal]


Epidemiology of human respiratory viruses in children with acute respiratory tract infection in a 3-year hospital-based survey in Northern Italy.

Diagn Microbiol Infect Dis 2019 Jan 17. Epub 2019 Jan 17.

Department of Medicine and Surgery, University of Parma, Viale A. Gramsci 14, 43126 Parma, Italy.

Acute respiratory tract infections (ARTIs) are among the leading causes of morbidity and mortality in children. The viral etiology of ARTIs was investigated over 3 years (October 2012-September 2015) in 2575 children in Parma, Italy, using indirect immunofluorescent staining of respiratory samples for viral antigens, cell culture, and molecular assays. Respiratory viruses were detected in 1299 cases (50. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.008DOI Listing
January 2019
1 Read

Pitfalls in big data analysis: next-generation technologies, last-generation data.

Diagn Microbiol Infect Dis 2018 Dec 15. Epub 2018 Dec 15.

Department of Microbiology, The University of Hong Kong, Hong Kong, China; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong, China. Electronic address:

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.006DOI Listing
December 2018

Establishment of a comprehensive and high throughput serological algorithm for Zika virus diagnostic testing.

Diagn Microbiol Infect Dis 2019 Jan 14. Epub 2019 Jan 14.

Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada. Electronic address:

The previous serological algorithm for Zika virus (ZIKV) comprised screening by anti-ZIKV IgM capture ELISA (MAC-ELISA) for samples collected within 3 months postexposure or onset (MPEO). Samples positive by MAC-ELISA and samples collected beyond 3 MPEO were tested by the confirmatory plaque reduction neutralization test (PRNT), which proved laborious and time-consuming during the 2015 outbreak. Thus, we evaluated several ZIKV ELISAs to establish an anti-IgM and anti-IgG combination for use as a screening tool for all samples prior to PRNT confirmation. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.004DOI Listing
January 2019
2.457 Impact Factor

Evaluation of the Abbott ARCHITECT™ cytomegalovirus IgM/IgG, rubella IgM/IgG, and syphilis treponemal antibodies enzyme immunoassays in a mother and child health center population.

Diagn Microbiol Infect Dis 2019 Jan 17. Epub 2019 Jan 17.

Université de Montréal, 2900 Boulevard Édouard-Montpetit, Montréal, Québec, Canada, H3T 1J4; Centre Hospitalier Universitaire Sainte-Justine, 3175 Chemin de la Côte Sainte-Catherine, Montreal, Quebec, Canada, H3T 1C4.

This study evaluated the concordance of Architect™ chemiluminescent microparticle immunoassays with Captia™ ELISA for cytomegalovirus (CMV) IgM and IgG, with Enzygnost™ and Captia™ ELISA for rubella IgM and IgG and with Trep-Sure™ ELISA for syphilis treponemal antibodies in a mixed pediatric and obstetrical population. Total agreement between assays and Kappa statistic value were 82.5% (95% CI: 75. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07328893193002
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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.017DOI Listing
January 2019
2 Reads

Microbiology of polymicrobial prosthetic joint infection.

Diagn Microbiol Infect Dis 2019 Jan 15. Epub 2019 Jan 15.

Divisions of Clinical Microbiology, Mayo Clinic, Rochester, MN; Infectious Diseases, Mayo Clinic, Rochester, MN. Electronic address:

Prosthetic joint infection (PJI) is a rare but challenging complication of arthroplasty. Herein, we describe the epidemiology and microbiology of PJI, with a focus on analyzing differences between the microbiology of polymicrobial versus monomicrobial infection of hip, knee, and shoulder prostheses. In addition, we report the most frequent co-pathogens in polymicrobial infections, as detected by culture. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.006DOI Listing
January 2019
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Comparison of FTD® respiratory pathogens 33 and a singleplex CDC assay for the detection of respiratory viruses: A study from Cameroon.

Diagn Microbiol Infect Dis 2019 Jan 16. Epub 2019 Jan 16.

Department of Virology, Centre Pasteur of Cameroon, 451 Rue 2005, P.O. Box 1274, Yaoundé, Cameroon. Electronic address:

Introduction: This study compares the detection of 14 common respiratory viruses by two different real-time reverse transcription polymerase chain reaction (rRT-PCR) methods: in house singleplex tests developed by the Centers for Disease Control and Prevention and the commercially available Fast Track Diagnostic (FTD®) Respiratory Pathogens 33 multiplex test.

Methods: A total of 217 nasopharyngeal swabs were analyzed using CDC singleplex rRT-PCR and FTD® Respiratory Pathogens 33 multiplex assays, for the detection of 14 respiratory viruses.

Results: The results showed that 179/217 (82. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.007DOI Listing
January 2019

Rapid microarray-based assay for detection of pyrazinamide resistant Mycobacterium tuberculosis.

Diagn Microbiol Infect Dis 2018 Dec 30. Epub 2018 Dec 30.

Alere Technologies GmbH, Jena, Germany.

Pyrazinamide (PZA) is a key antibiotic for the treatment of drug susceptible tuberculosis. PZA-resistance is mainly mediated by mutations in the pncA gene; however the current gold standard is a phenotypic drug susceptibility test requiring a well-adjusted pH-value for reliable results. Our melting curve assay detects a non-wild type genotype in selected pncA regions in at least 3750 gene copies/mL within 2. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.011DOI Listing
December 2018

Efficacy of a 14-day course of amoxicillin for patients with erythema migrans.

Diagn Microbiol Infect Dis 2019 Jan 10. Epub 2019 Jan 10.

Division of Infectious Diseases, New York Medical College, Valhalla, NY 10595.

Although a 14-day treatment course with amoxicillin is in wide clinical usage to treat early Lyme disease, only a few published studies exist to validate its efficacy and safety, with none in the United States. In this study, we reviewed the records of 24 prospectively followed adult patients with erythema migrans who were prescribed a 14-day course of amoxicillin, 500 mg 3 times daily. Treatment with amoxicillin was well tolerated and uniformly successful in resolving the erythema migrans skin lesion and in preventing the development of an objective neurologic, cardiac, or rheumatologic manifestation. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.003DOI Listing
January 2019
2 Reads

A multisite implementation of a real-time polymerase chain reaction assay to predict ciprofloxacin susceptibility in Neisseria gonorrhoeae.

Diagn Microbiol Infect Dis 2019 Jan 14. Epub 2019 Jan 14.

UCLA Division of Infectious Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.

There are no commercially available Food and Drug Administration-cleared rapid tests for Neisseria gonorrhoeae antimicrobial susceptibility testing. This study evaluated the performance of a laboratory-developed real-time polymerase chain reaction assay for genotyping the gyrA gene to determine antimicrobial susceptibility to ciprofloxacin. Validation and clinical performance of the gyrA assay were evaluated across 3 geographic locations (Los Angeles, San Francisco, Philadelphia). Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.018DOI Listing
January 2019

Performance of the Accelerate Diagnostics Pheno system with resin-containing BacT/ALERT® Plus blood culture bottles.

Diagn Microbiol Infect Dis 2019 Jan 16. Epub 2019 Jan 16.

Clinical Microbiology Laboratory, Department of Pathology, Duke University Health System. Electronic address:

Bacteremia and septicemia require rapid identification (ID) and antimicrobial susceptibility testing (AST) to start targeted, appropriate therapy. To answer this need, Accelerate Diagnostics, Inc., developed the Accelerate Pheno™ system (AXDX), a fast ID and phenotypic AST platform. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.002DOI Listing
January 2019

CHROMagar™ orientation urine culture medium produces matrix-assisted laser desorption ionization-time-of-flight mass spectrometry spectra misidentified as Mycoplasma arginini and Mycoplasma alkalescens.

Diagn Microbiol Infect Dis 2019 Jan 2. Epub 2019 Jan 2.

Shared Health, Diagnostic Services, Clinical Microbiology, Winnipeg, Manitoba, Canada; Department of Medical Microbiology and Infectious Diseases, College of Medicine, University of Manitoba. Winnipeg, Manitoba, Canada.

Matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry is commonly used to identify bacteria and yeasts. Studies indicate that MALDI-TOF is relatively indifferent to the medium used for culture. We report on an investigation into high- and low-confidence MALDI-TOF misidentifications of Mycoplasma arginini and Mycoplasma alkalescens from urine specimens plated to CHROMagar™ Orientation medium that appear to be due to the intrinsic mass spectrum of the medium. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.014DOI Listing
January 2019

Monomicrobial gram-negative necrotizing fasciitis: An uncommon but fatal syndrome.

Diagn Microbiol Infect Dis 2019 Jan 4. Epub 2019 Jan 4.

Division of Infectious Diseases, Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Soonchunhyang University College of Medicine, Seoul, Republic of Korea. Electronic address:

This study aimed to characterize patients with monomicrobial gram-negative necrotizing fasciitis in three university hospitals in Korea. In this study, of the 115 patients with community-acquired necrotizing fasciitis, 67 (58%) had monomicrobial infections: 31 (27%) in the gram-negative group and 36 (31%) in the gram-positive group. The majority of patients in the gram-negative group were infected with Escherichia coli followed by Klebsiella pneumoniae and Vibrio vulnificus. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.013DOI Listing
January 2019
1 Read

Rapid antimicrobial susceptibility testing by VITEK®2 directly from blood cultures in patients with Gram-negative rod bacteremia.

Diagn Microbiol Infect Dis 2019 Jan 16. Epub 2019 Jan 16.

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA; Clinical Microbiology Laboratory, Stanford University Medical Center, Palo Alto, CA, USA; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA. Electronic address:

Objective: Optimizing therapy for bacteremia is currently limited by the 1-2-day turnaround time required for antimicrobial susceptibility testing (AST). Here, we assess a rapid AST method with VITEK®2 (bioMérieux, France) directly from positive blood cultures.

Methods: Patient-derived positive blood cultures with Gram-negative rods identified as Enterobacteriaceae and Pseudomonas aeruginosa were prospectively tested, and other blood culture bottles were spiked with carbapenem-resistant Enterobacteriaceae (CRE). Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.001DOI Listing
January 2019

Optimization of a rapid test for antibodies to the Chlamydia trachomatis antigen Pgp3.

Diagn Microbiol Infect Dis 2018 Nov 10. Epub 2018 Nov 10.

Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address:

Serological surveillance for trachoma could allow monitoring of transmission levels in areas that have achieved elimination targets. Platforms that allow testing in basic laboratories or testing of easy-to-manage samples such as dried blood spots would contribute to the feasibility of serologic testing. Blood from 506 1-12-year-olds in 2 villages in Kongwa district, Tanzania, was tested for antibodies against the antigen Pgp3. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.001DOI Listing
November 2018

Field evaluation of a dual rapid Human Immunodeficiency Virus and treponemal syphilis rapid test in community-based clinics in Los Angeles and New York.

Diagn Microbiol Infect Dis 2018 Oct 11. Epub 2018 Oct 11.

David Geffen School of Medicine at UCLA, 10920 Wilshire Blvd, Los Angeles, CA, 90024, USA.

Introduction: Dual human immunodeficiency virus/syphilis rapid diagnostic devices can play an important role in prevention efforts. The field performance of the INSTI Multiplex HIV-1/HIV-2/Syphilis Antibody Test (Multiplex) was evaluated.

Methods: Fingerstick whole blood was tested using the rapid test. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.10.002DOI Listing
October 2018
2 Reads

GDH and toxin immunoassay for the diagnosis of Clostridioides (Clostridium) difficile infection is not a 'one size fit all' screening test.

Diagn Microbiol Infect Dis 2018 Dec 29. Epub 2018 Dec 29.

Department of Pathology, Keck School of Medicine of the University of Southern California, Los Angeles, CA, USA. Electronic address:

Diagnosing Clostridioides (Clostridium) difficile infection is challenged by lack of a clear gold standard. We sought to determine if the two-step algorithm (screening GDH and toxin lateral flow assay followed by tcdB PCR) would have adequate clinical performance at a tertiary care center. Of 486 patients, 310 (63. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.010DOI Listing
December 2018
1 Read

Microbiology of combat-related extremity wounds: Trauma Infectious Disease Outcomes Study.

Diagn Microbiol Infect Dis 2018 Dec 29. Epub 2018 Dec 29.

Infectious Disease Clinical Research Program, Preventive Medicine & Biostatistics Department, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd, Bethesda, MD, 20814, USA.

We present extremity wound microbiology data from 250 combat casualties (2009-2012). Confirmed extremity wound infections (EWIs) were based on clinical and laboratory findings. Suspected EWIs had isolation of organisms from wound cultures with associated signs/symptoms not meeting clinical diagnostic criteria. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.008DOI Listing
December 2018

Proposed nomenclature or classification changes for bacteria of medical importance: Taxonomic Update 4.

Authors:
J Michael Janda

Diagn Microbiol Infect Dis 2018 Dec 30. Epub 2018 Dec 30.

Kern County Public Health Laboratory, Public Health Services Department, Bakersfield, CA 93306-3302, USA. Electronic address:

A key aspect of medical, public health, and diagnostic microbiology laboratories is the accurate identification and rapid reporting and communication to medical staff regarding patients with infectious agents of clinical importance. Microbial taxonomy continues to change at a very rapid rate in the era of molecular diagnostics including whole genome sequencing. This update focuses on taxonomic changes and proposals that may be of medical importance for years 2017 and 2018. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.009DOI Listing
December 2018

Clinical features, identification, antimicrobial resistance patterns of Nocardia species in China: 2009-2017.

Diagn Microbiol Infect Dis 2018 Dec 29. Epub 2018 Dec 29.

Laboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Beijing, China. Electronic address:

Nocardia spp. is a pathogen responsible for a variety of clinical infections, ranging from skin and soft tissue infections, to the respiratory tract and central nervous system infections. Its epidemiological characteristics, including species distribution, clinical features, and antimicrobial susceptibility profiles, should be under surveillance for the prevention and treatment of nocardiosis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07328893183080
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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.007DOI Listing
December 2018
5 Reads

Protracted course of disseminated adenovirus disease with necrotizing granulomas in the liver.

Diagn Microbiol Infect Dis 2019 Jan 2. Epub 2019 Jan 2.

Division of Infectious Diseases, Mayo Clinic Hospital, 5777 East Mayo Boulevard, Phoenix, AZ 85054, USA.

A 52-year-old male with chronic lymphocytic leukemia was hospitalized with disseminated adenovirus disease. More than a month following recovery, hepatic necrotizing granulomas secondary to adenovirus were found. This case illustrates the protracted course that adenovirus disease may take and emphasizes an unusual presentation with hepatic necrotizing granulomas. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.012DOI Listing
January 2019

In vitro evaluation of antifungal combination against Cryptococcus neoformans.

Diagn Microbiol Infect Dis 2018 Dec 8. Epub 2018 Dec 8.

Postgraduate Program in Pharmaceutical Sciences, Health Sciences Centre, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.

This study evaluated combinations of amphotericin B with anidulafungin, caspofungin, and micafungin against 30 clinical isolates of Cryptococcus neoformans following the CLSI M27-A3 and the checkerboard microdilution method. The combination amphotericin B + micafungin showed 60% of synergistic effect against C. neoformans, while most of the other interactions were indifferent. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.003DOI Listing
December 2018

In vitro activity of Plazomicin against Enterobacteriaceae isolates carrying genes encoding aminoglycoside-modifying enzymes most common in US Census divisions.

Diagn Microbiol Infect Dis 2018 Dec 5. Epub 2018 Dec 5.

JMI Laboratories, North Liberty, IA 52317.

Aminoglycoside-nonsusceptible isolates of Escherichia coli, Klebsiella, Proteus, and Enterobacter species (480/3675) from US hospitals collected during 2014-2015 were screened for 16S rRNA methyltransferase and aminoglycoside-modifying enzyme (AME) genes. Only 5 isolates had high aminoglycoside MICs and carried 16S rRNA methyltransferases. AME genes were observed among 89. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.10.023DOI Listing
December 2018
2 Reads

In vitro activity of flomoxef against extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Korea.

Diagn Microbiol Infect Dis 2018 Nov 26. Epub 2018 Nov 26.

Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, South Korea.

To find an alternative regimen for the treatment of extended-spectrum β-lactamase (EBSL)-producing Enterobacteriaceae infections, we examined the in vitro activity of flomoxef against Escherichia coli and Klebsiella pneumoniae having CTX-M-1 group and/or CTX-M-9 group ESBLs. Boronic acid disk methods and polymerase chain reaction amplification were used to detect for ESBL, and AmpC β-lactamase and AmpC β-lactamase co-producers were excluded. Minimum inhibitory concentrations (MICs) were determined for flomoxef by broth microdilution. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.017DOI Listing
November 2018
1 Read

Evaluation of the Luminex ARIES® system for the detection and quantification of BK virus (BKV) DNA in plasma samples from kidney transplant recipients.

Diagn Microbiol Infect Dis 2018 Dec 15. Epub 2018 Dec 15.

Quantigen Genomics Services, Fishers, IN. Electronic address:

BK virus (BKV) nephropathy is a serious complication in renal transplant recipients due to the need for immunosuppression. Nearly 50% of renal transplant patients with BKV nephropathy experience a significant loss of function of the transplanted kidney. It is routine practice to screen renal transplant recipients regularly for BK viremia. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.004DOI Listing
December 2018
4 Reads

Higher C6 enzyme immunoassay index values correlate with a diagnosis of noncutaneous Lyme disease.

Diagn Microbiol Infect Dis 2018 Dec 8. Epub 2018 Dec 8.

Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA.

The correlation between the Food and Drug Administration-cleared C6 enzyme immunoassay (EIA) C6 index values and a diagnosis of Lyme disease has not been examined. We used pooled patient-level data from 5 studies of adults and children with Lyme disease and control subjects who were tested with the C6 EIA. We constructed a receiver operating characteristic curve using regression clustered by study and measured the area under the curve (AUC) to examine the accuracy of the C6 index values in differentiating between patients with noncutaneous Lyme disease and control subjects. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.001DOI Listing
December 2018
3 Reads

Dual rapid test for HIV and syphilis: A laboratory evaluation of the diagnostic accuracy of the Standard Q HIV/Syphilis Combo Test.

Diagn Microbiol Infect Dis 2018 Nov 24. Epub 2018 Nov 24.

Division of Infectious Diseases, Department of Medicine, University of California Los Angeles, Los Angeles, CA, USA; Department of Epidemiology, Fielding School of Public Health, University of California Los Angeles, Los Angeles, CA, USA.

We evaluated the Standard Q HIV/Syphilis Combo Test (SD Biosensor, South Korea), a dual rapid test using stored sera (N = 400) in a laboratory setting in Lima, Peru. The sensitivity and specificity for HIV antibody detection was 100.0% (95% CI: 98. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.018DOI Listing
November 2018
1 Read

Genetic characterization of multiple NDM-1-producing clinical isolates in Mexico.

Diagn Microbiol Infect Dis 2018 Dec 10. Epub 2018 Dec 10.

Instituto Nacional de Salud Pública (INSP), CISEI, Laboratorio de Resistencia Bacteriana, Cuernavaca, Morelos, Mexico. Electronic address:

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.002DOI Listing
December 2018
1 Read

Antenatal and intrapartum nucleic acid amplification test use for group B Streptococcus screening-United States, 2016.

Diagn Microbiol Infect Dis 2018 Dec 11. Epub 2018 Dec 11.

Respiratory Diseases Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30329, USA. Electronic address:

Perinatal group B Streptococcus (GBS) disease prevention guidelines in 2010 allowed for processing of screening specimens by nucleic acid amplification tests (NAATs); however, the extent of NAAT use is unknown. A 2016 laboratory survey sent to 10 surveillance sites found that 18.7% of responding laboratories offered NAAT for GBS screening (antenatal only: 7. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07328893183052
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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.026DOI Listing
December 2018
4 Reads

Factors associated with the recurrence of acute pyelonephritis caused by extended-spectrum β-lactamase-producing Escherichia coli: the importance of infectious disease consultation.

Diagn Microbiol Infect Dis 2018 Nov 29. Epub 2018 Nov 29.

Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea. Electronic address:

To investigate factors for the recurrence of acute pyelonephritis (APN) caused by extended-spectrum β-lactamase-producing Escherichia coli (ESBL-PE), a retrospective cohort study was performed at a tertiary-care hospital from 2014 through 2016. Among 135 patients who had recovered from APN caused by ESBL-PE, the 1-year cumulative recurrence rates of APN caused by any pathogen or by ESBL-PE specifically were 35.6% and 22. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.019DOI Listing
November 2018
3 Reads

Antimicrobial activity of ceftolozane-tazobactam tested against gram-negative contemporary (2015-2017) isolates from hospitalized patients with pneumonia in US medical centers.

Diagn Microbiol Infect Dis 2018 Nov 30. Epub 2018 Nov 30.

JMI Laboratories, North Liberty, IA 52317.

Pseudomonas aeruginosa (n = 1531) and Enterobacteriaceae (n = 2373) clinical isolates from hospitalized patients with pneumonia were collected from 31 US medical centers during 2015-2017. Isolates were susceptibility tested against ceftolozane-tazobactam and comparators by broth microdilution. Results from intensive care unit (ICU) patients and patients with ventilator-associated bacterial pneumonia (VABP) were analyzed separately. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.021DOI Listing
November 2018
2 Reads

Plasma and cerebrospinal fluid population pharmacokinetic modeling and simulation of meropenem after intravenous and intrathecal administration in postoperative neurosurgical patients.

Diagn Microbiol Infect Dis 2018 Aug 17. Epub 2018 Aug 17.

Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China; Precision Medicine Research Center for Neurological Disorders, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China. Electronic address:

Combined intravenous and local intrathecal administration of meropenem in patients after craniotomy is widely used to treat intracranial infections. However, the optimal dosing regimen of meropenem has not been investigated, posing a risk to treatment efficacy. We aimed to identify significant factors associated with inter-individual variability in cerebrospinal fluid (CSF) pharmacokinetics of meropenem and to evaluate potential intravenous and intrathecal meropenem dosing regimens for the treatment of patients with intracranial infections. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.08.003DOI Listing
August 2018
1 Read

Synergy of polymyxin B, tigecycline and meropenem against carbapenem-resistant Enterobacter cloacae complex isolates.

Diagn Microbiol Infect Dis 2018 Nov 22. Epub 2018 Nov 22.

Laboratório de Pesquisa em Resistência Bacteriana - LABRESIS, Hospital de Clínicas de Porto Alegre (HCPA), 2350 Ramiro Barcelos St, Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Microbiologia Agrícola e do Ambiente - Universidade Federal do Rio Grande do Sul (UFRGS), 500 Sarmento Leite St, Porto Alegre, Brazil. Electronic address:

Here, we evaluated the combinations of antibiotics polymyxin B (PMB), tigecycline (TGC) and meropenem (MEM) by time-kill curves (TKC) against carbapenem-resistant Enterobacter cloacae isolates. Combination of PMB/TGC and PMB/MEM showed promising results in sub-inhibitory concentration of PMB indicating the possibility of reducing the dose of PMB used in the treatment. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.011DOI Listing
November 2018
1 Read

Comparison of the Idylla™ Respiratory (IFV-RSV) panel with the GeneXpert Xpert® Flu/RSV assay: a retrospective study with nasopharyngeal and midturbinate samples.

Diagn Microbiol Infect Dis 2018 Dec 4. Epub 2018 Dec 4.

Department of Microbiology, Heilig Hart Hospital Lier, Mechelsestraat 24, 2500 Lier, Belgium. Electronic address:

The objective of this study was to compare the performance of the Idylla™ Respiratory (IFV-RSV) panel to the GeneXpert Xpert® Flu/RSV assay and establish the performance of a midturbinate swab compared to nasopharyngeal sampling. Considering GeneXpert® assay as imperfect reference standard, a positive percentage agreement between both assays of 98-100% for influenza A and 96-99% for influenza B could be calculated when 354 nasopharyngeal and 325 midturbinate swabs were retrospectively analyzed. Comparing midturbinate samples to nasopharyngeal specimens of 321 subjects, positive percentage agreement varied from 42% to 94% depending on both target virus and assay used. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.022DOI Listing
December 2018
1 Read

Frequency of 16S ribosomal RNA methyltransferase detection among Escherichia coli and Klebsiella pneumoniae clinical isolates obtained from patients in Canadian hospitals (CANWARD, 2013-2017).

Diagn Microbiol Infect Dis 2018 Dec 14. Epub 2018 Dec 14.

Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada.

Pan-aminoglycoside (amikacin, gentamicin, tobramycin, plazomicin)-resistant Escherichia coli and Klebsiella pneumoniae clinical isolates from patients in Canadian Hospitals (2013-2017) were evaluated by whole genome sequencing for 16S ribosomal RNA methyltransferase genes. The rmtB gene was detected in 2 isolates (1 of 3094 E. coli [0. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.005DOI Listing
December 2018
2 Reads

Nephrotoxicity prevalence in patients treated with polymyxins: a systematic review with meta-analysis of observational studies.

Diagn Microbiol Infect Dis 2018 Nov 22. Epub 2018 Nov 22.

Center for Medical and Pharmaceutical Sciences, Universidade Estadual do Oeste do Paraná, Cascavel, Brazil. Electronic address:

Colistin and polymyxin B are increasingly reintroduced in clinical practice due to the absence of effective antibiotics for the treatment of emerging infections caused by gram-negative bacteria. The synthesis of current evidence on the characteristics of polymyxins, especially regarding nephrotoxicity, is necessary. This study aims to conduct a systematic review and meta-analysis of cohort-type observational studies in order to identify the prevalence of nephrotoxicity in patients treated with either colistin or polymyxin B. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.008DOI Listing
November 2018
1 Read

Evaluating the preservation and isolation of stool pathogens using the COPAN FecalSwab™ Transport System and Walk-Away Specimen Processor.

Diagn Microbiol Infect Dis 2018 Dec 11. Epub 2018 Dec 11.

Department of Microbiology, University Health Network/Sinai Health System, Toronto, Ontario, M5G 1X5, Canada.

The isolation of stool pathogens is difficult due to their fastidious nature and the rapid overgrowth of fecal flora. In this study, we evaluate the preservation and isolation of enteric pathogens from stool using the automated COPAN Walk-Away Specimen Processor (WASP®) in conjunction with FecalSwab™ and selenite media. Pathogen viability and fecal commensal abundance were stable in FecalSwab™ media under both room-temperature and refrigerated incubation conditions, resulting in a significantly increased number of well-isolated pathogen colonies observed when compared to samples incubated in modified Cary-Blair media. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.020DOI Listing
December 2018
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Factors associated with sequelae after treatment of hematogenous pyogenic vertebral osteomyelitis.

Diagn Microbiol Infect Dis 2018 Dec 4. Epub 2018 Dec 4.

Division of Infectious Diseases, Department of Internal Medicine, Kyung Hee University Hospital, Kyung Hee University School of Medicine, Seoul, Republic of Korea. Electronic address:

Objectives: Functional disability may persist after completing treatment for hematogenous pyogenic vertebral osteomyelitis (HPVO). The objective of this study was to identify factors associated with residual sequelae after treatment of HPVO.

Methods: We conducted a retrospective study of patients diagnosed with HPVO at 5 tertiary-care hospitals between January 2005 and December 2012. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.024DOI Listing
December 2018
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Elizabethkingia miricola: Discrepancies in identification and antimicrobial susceptibilities.

Diagn Microbiol Infect Dis 2018 Dec 5. Epub 2018 Dec 5.

Microbiology Department, Shah SatnamJi Speciality Hospitals, Sirsa, Haryana, India. Electronic address:

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.025DOI Listing
December 2018
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Quadruple valve infective endocarditis presenting with suspected Austrian syndrome: a case report and a case series of quadruple valve infective endocarditis.

Diagn Microbiol Infect Dis 2018 Dec 4. Epub 2018 Dec 4.

Division of Infectious Diseases, Changi General Hospital, 2 Simei Street 3, Singapore 529889.

Objectives: Austrian syndrome comprises the triad of pneumonia, meningitis, and endocarditis secondary to Streptococcus pneumonia. We present what we believe to be the first reported case of Austrian syndrome with quadruple heart valve involvement and review the literature detailing cases of quadruple valve infective endocarditis.

Case Presentation And Results: A case is presented of a patient with radiographic evidence of a left lower lobe pneumonia. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.023DOI Listing
December 2018
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Minimal inhibitory concentration of omadacycline and doxycycline against bacterial isolates with known tetracycline resistance determinants.

Diagn Microbiol Infect Dis 2018 Nov 29. Epub 2018 Nov 29.

Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.

Omadacycline is an aminomethylcycline derived from the tetracycline class. The minimum inhibitory concentration of 115 Enterobacteriaceae and Staphylococcus aureus isolates with known tetracycline resistance determinants against omadacycline and doxycycline was determined by broth microdilution. Omadacycline is, on a weight basis, more active than doxycycline for nearly all isolates, and differences in activity correlated with organism rather than resistance mechanism. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.010DOI Listing
November 2018
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Characterization of novel Mycobacterium tuberculosis pncA gene mutations in clinical isolates from the Ukraine.

Diagn Microbiol Infect Dis 2018 Nov 10. Epub 2018 Nov 10.

Longhorn Vaccines and Diagnostics, San Antonio, TX, USA.

Multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis cases in the Ukraine are increasing. Pyrazinamide (PZA) is critically important for first- and second-line tuberculosis (TB) treatment regimes. However, PZA drug susceptibility testing is time consuming and technically challenging. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07328893183058
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http://dx.doi.org/10.1016/j.diagmicrobio.2018.10.018DOI Listing
November 2018
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Genetic mutations in linezolid-resistant Mycobacterium avium complex and Mycobacterium abscessus clinical isolates.

Diagn Microbiol Infect Dis 2018 Nov 30. Epub 2018 Nov 30.

Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea. Electronic address:

There are no studies evaluating the mechanisms driving linezolid resistance in nontuberculous mycobacteria. The novel mutations G2599A and A2137T in the 23S rRNA gene and mutations A439G and G443A in the rplD gene associated with linezolid resistance were identified in linezolid-resistant M. avium complex isolates. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.10.022DOI Listing
November 2018
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Near point-of-care adoption of Cepheid Xpert® Flu/RSV XC testing within an integrated healthcare delivery network.

Diagn Microbiol Infect Dis 2018 Nov 23. Epub 2018 Nov 23.

Geisinger, Center for Infectious Diseases Diagnostics and Research, 100 N Academy Ave., Danville, PA. 17822-2619; Geisinger Diagnostics Medicine Institute, 100 N Academy Ave., Danville, PA. 17822-0131. Electronic address:

Flu/RSV testing was implemented in out-patient clinic-based physician office laboratories throughout Pennsylvania. On-site testing reduced the collect-to-result time by 70% when compared to testing in a centralized core laboratory; over- or under-treatment for influenza A and B (measured by anti-viral prescription) was reduced by 15% (P < 0.0001). Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.013DOI Listing
November 2018
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Identification and detection of USA300 methicillin-resistant Staphylococcus aureus clones with a partial deletion in the ccrB2 gene on the type IV SCCmec element.

Diagn Microbiol Infect Dis 2018 Nov 24. Epub 2018 Nov 24.

Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.

Panton-Valentine leukocidin-positive highly pathogenic USA300 methicillin-resistant Staphylococcus aureus carries type IV staphylococcal cassette chromosome (SCC) mec. Here, we found USA300-like strains (named as ΨUSA300), which could not be identified as SCCmec type IV by the conventional PCR method due to a 12 bp deletion on ccrB2. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.016DOI Listing
November 2018
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Pathogen enrichment from human whole blood for the diagnosis of bloodstream infection: Prospects and limitations.

Diagn Microbiol Infect Dis 2018 Nov 26. Epub 2018 Nov 26.

Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500 Krems, Austria; Christian Doppler Laboratory for Innovative Therapy Approaches in Sepsis, Department for Biomedical Research, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500 Krems, Austria. Electronic address:

Blood culture represents the current reference method for the detection of bacteria or fungi in the circulation. To accelerate pathogen identification, molecular diagnostic methods, mainly based on polymerase chain reaction (PCR), have been introduced to ensure early and targeted antibiotic treatment of patients suffering from bloodstream infection. Still, these approaches suffer from a lack of sensitivity and from inhibition of PCR in a number of clinical samples, leading to false negative results. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.015DOI Listing
November 2018
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Factors influencing cytomegalovirus DNA load measurements in whole blood and plasma specimens from allogeneic hematopoietic stem cell transplant recipients.

Diagn Microbiol Infect Dis 2018 Nov 22. Epub 2018 Nov 22.

Microbiology Service, Hospital Clínico Universitario, Instituto de Investigación INCLIVA, Valencia, Spain; Department of Microbiology, School of Medicine, University of Valencia, Valencia, Spain. Electronic address:

We assessed the impact of several parameters, including the nature of the episode of Cytomegalovirus (CMV) DNAemia, the use of preemptive antiviral therapy, and the blood cell content in CMV DNA loads measured in whole blood (WB) and plasma (PL). CMV DNA load was quantified in 245 paired specimens collected within 43 postengraftment episodes of CMV DNAemia by using the CMV RealTime CMV PCR (Abbott Molecular). Concordant categorical results were obtained for 78. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.012DOI Listing
November 2018
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Impact of bacterial and viral coinfection in community-acquired pneumonia in adults.

Diagn Microbiol Infect Dis 2018 Nov 24. Epub 2018 Nov 24.

Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Republic of Korea. Electronic address:

Bacterial and viral coinfected community-acquired pneumonia (CAP) is poorly characterized in adults. The aim of this study was to investigate the influence of bacterial and viral coinfection in patients with CAP. A total of 235 adults who requested molecular tests of pneumonia and were diagnosed with CAP were enrolled in this study. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07328893183066
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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.014DOI Listing
November 2018
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Polymyxins susceptibility tests: we need talk about a solution.

Diagn Microbiol Infect Dis 2018 Nov 22. Epub 2018 Nov 22.

Hospital das Clínicas, LIM03-Divisão Laboratório Central, Faculdade de Medicina Universidade de São Paulo, São Paulo, Brazil.

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.09.018DOI Listing
November 2018
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Impact of short-incubation MALDI-TOF MS on empiric antibiotic therapy in bloodstream infections caused by Pseudomonas aeruginosa, Enterococcus spp. and AmpC-producing Enterobacteriaceae.

Diagn Microbiol Infect Dis 2018 Nov 22. Epub 2018 Nov 22.

Department of Infectious Diseases, Inflammation Center, Helsinki University Hospital and University of Helsinki, Finland.

The impact of the short-incubation matrix-assisted laser desorption ionization time-of-flight (si-MALDI-TOF) mass spectrometry technique was evaluated in the treatment of bloodstream infections (BSIs) caused by Pseudomonas aeruginosa, Enterococcus spp., and Amp-C producing Enterobacteriaceae. A total of 124 bacteremia episodes were divided into 2 groups: i) si-MALDI-TOF group (n = 69) and ii) control group (n = 55). Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.009DOI Listing
November 2018
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Bactericidal activity of bacteriophage endolysin HY-133 against Staphylococcus aureus in comparison to other antibiotics as determined by minimum bactericidal concentrations and time-kill analysis.

Diagn Microbiol Infect Dis 2018 Nov 20. Epub 2018 Nov 20.

Institute of Medical Microbiology, University Hospital Münster, Münster, Germany. Electronic address:

Methicillin-resistant Staphylococcus aureus (MRSA) decolonization is expensive and time consuming, and new agents are necessary due to increasing resistance rates. The administration of bacteriophages or particularly their endolysins may offer an alternative treatment strategy and could provide a solution to overcome the selection pressure due to classical antibiotics. Here, the bactericidal activity was characterized for the recombinant chimeric bacteriophage endolysin HY-133 in comparison to other antimicrobials. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.11.005DOI Listing
November 2018
1 Read