6,020 results match your criteria Diagnostic microbiology and infectious disease[Journal]


A selective culture medium for screening linezolid-resistant gram-positive bacteria.

Diagn Microbiol Infect Dis 2019 Mar 21. Epub 2019 Mar 21.

Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (IAME, France), University of Fribourg; Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), Fribourg, Switzerland.

The SuperLinezolid medium was developed for screening resistance to linezolid (LZD) in Gram-positive bacteria (Staphylococcus spp., Enterococcus spp.). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07328893193009
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http://dx.doi.org/10.1016/j.diagmicrobio.2019.03.006DOI Listing
March 2019
1 Read

The use of genomic DNA sequences as type material for valid publication of bacterial species names will have severe implications for clinical microbiology and related disciplines.

Diagn Microbiol Infect Dis 2019 Mar 22. Epub 2019 Mar 22.

Diagnostic Laboratory for Bacteriology and Parasitology, Center for Infectious Disease Research, Diagnostics and laboratory Surveillance, National Institute of Public Health and the Environment, Bilthoven, The Netherlands.

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.03.007DOI Listing

Clinical evaluation of IntelliPlex HCV genotyping kit for hepatitis C virus genotyping.

Diagn Microbiol Infect Dis 2019 Mar 12. Epub 2019 Mar 12.

PlexBio Co., Ltd., Neihu District, Taipei, Taiwan.

Genotyping of the hepatitis C virus (HCV) is crucial for determining the most efficient anti-viral therapy. The clinical sensitivity and specificity of the IntelliPlex HCV Genotyping Kit was determined by comparing the assay results of 307 specimens with the results obtained by Sanger sequencing. Out of 202 HCV-positive specimens tested, 8 samples yielded discrepant results between the IntelliPlex HCV Genotyping Kit and Sanger sequencing. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.020DOI Listing
March 2019
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Influenza B false-positive results by rapid molecular tests Alere™ i Influenza A&B 2 in France.

Diagn Microbiol Infect Dis 2019 Mar 13. Epub 2019 Mar 13.

Service de microbiologie, Hôpital d'Instruction des Armées Sainte Anne, Toulon, France; Ecole du Val-de-Grâce, Paris, France. Electronic address:

The Alere-i™ Influenza A&B (Abbott), a nicking endonuclease amplification reaction test, has recently been improved in order to deliver results in a few minutes. Our field observation highlights two problems with this new version: improper interpretation of a test as valid despite improper reagent hydration and falsely influenza B positive results. We advise users of the new system to check reagent hydration prior to reporting a result and to systematically confirm positive influenza B results. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07328893193009
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http://dx.doi.org/10.1016/j.diagmicrobio.2019.03.002DOI Listing
March 2019
6 Reads

First reported human isolation of Staphylococcus delphini.

Diagn Microbiol Infect Dis 2019 Jan 26. Epub 2019 Jan 26.

Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, New York, NY, USA; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA. Electronic address:

The Staphylococcus intermedius group is a collection of coagulase-positive staphylococci composed of 5 members, including Staphylococcus pseudintermedius, a zoonotic pathogen often associated with exposure to dogs, and Staphylococcus delphini, which has not previously been recovered from humans. Here, we describe the first human case of S. delphini infection. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.014DOI Listing
January 2019
2 Reads

Subtyping of plasmid-mediated quinolone resistance among Salmonella serotypes by whole genome sequencing.

Diagn Microbiol Infect Dis 2019 Feb 21. Epub 2019 Feb 21.

Adolfo Lutz Institute, São Paulo, Brazil. Electronic address:

Most known plasmids are identified by conferring virulence or antimicrobial resistance phenotypes and such characteristics aid in the success of the dispersion of different plasmid types between bacteria from different sources. This study aimed to perform the subtyping of the plasmid-mediated quinolone resistance, detected in Salmonella spp. A total of 34 Salmonella strains non-susceptible to ciprofloxacin were evaluated. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.015DOI Listing
February 2019

Current significance of the Mycobacterium chelonae-abscessus group.

Diagn Microbiol Infect Dis 2019 Feb 10. Epub 2019 Feb 10.

Infectious Disease Department, Quest Diagnostics Nichols Institute, Chantilly, VA 20131. Electronic address:

Organisms of the Mycobacterium chelonae-abscessus group can be significant pathogens in humans. They produce a number of diseases including acute, invasive and chronic infections, which may be difficult to diagnose correctly. Identification among members of this group is complicated by differentiating at least eleven (11) known species and subspecies and complexity of identification methodologies. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.021DOI Listing
February 2019

Comparison of the microbiological efficacy of tedizolid and linezolid in acute bacterial skin and skin structure infections: pooled data from phase 3 clinical trials.

Diagn Microbiol Infect Dis 2019 Jan 30. Epub 2019 Jan 30.

Merck & Co., Inc., 4747 Executive Drive, San Diego, CA 92121, USA.

We evaluated the microbiological efficacy of tedizolid compared with that of linezolid against common and emerging pathogens using pooled data from 2 phase 3 trials (NCT01170221 and NCT01421511) in patients with acute bacterial skin and skin structure infections. Patients received tedizolid 200 mg once daily for 6 days (n = 664) or linezolid 600 mg twice daily for 10 days (n = 669). Favorable microbiological outcome in both treatment groups, defined as eradication or presumed eradication at the end of treatment and at the posttherapy evaluation, exceeded 85% for most pathogens, including methicillin-resistant Staphylococcus aureus. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.017DOI Listing
January 2019

Preliminary readings of antimicrobial susceptibility panels: A simple, fast and inexpensive way to detect bacterial resistance and enhance antibiotic treatment of bloodstream infections.

Diagn Microbiol Infect Dis 2019 Mar 7. Epub 2019 Mar 7.

UGC Biotecnología, Complejo Hospitalario Torrecárdenas, Servicio Andaluz de Salud, Almería, Spain.

Increasing incidence of resistant bacteria needs faster identification (ID) and antibiotic susceptibility testing (AST) in order to improve antimicrobial treatment of severe infections. We propose a preliminary reading of the AST MicroScan® panels coupled with mass spectrometry ID. A total of 157 bacterial clinical isolates were processed for routine ID and AST (in 22 cases, ID and AST were performed directly from positive blood culture bottles). Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.03.001DOI Listing
March 2019
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Migrating a lab-developed MERS-CoV real-time PCR to 3 "Sample to Result" systems: experiences on optimization and validation.

Diagn Microbiol Infect Dis 2019 Feb 10. Epub 2019 Feb 10.

Department of Laboratory Medicine, University Hospitals Leuven, Herestraat 49, 3000 Leuven, Belgium. Electronic address:

The goal of the study was to adapt our Middle East respiratory syndrome coronavirus (MERS-CoV) lab-developed test (LDT) to 3 "Sample to Result" (S2R) systems: BD MAX (BD), ELITe InGenius (ELITechGroup), and ARIES (Luminex). The BD MAX and InGenius system allowed use of lab-developed primers and TaqMan probes, while ARIES required conversion to MultiCode primers for melting curve analysis. Each device required ≤1 day of training and assay optimization. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.006DOI Listing
February 2019
3 Reads

Rapid diagnostic testing in the management of urinary tract infection: Potentials and limitations.

Diagn Microbiol Infect Dis 2019 Mar 5. Epub 2019 Mar 5.

Department of Urology, UCLA Medical Center, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, United States.

Urine culture and sensitivity (C&S) remains the gold standard diagnostic for urinary tract infections (UTI). To reduce the use of inaccurate or broad-spectrum empiric antimicrobials, rapid identification and quantification (IDQ) and antimicrobial susceptibility testing (AST) with results within 30 and 150 min, respectively, are under development. To assess the impact of rapid diagnostics, five UTI vignettes were constructed, and ninety-one United States physicians were surveyed regarding their diagnostic, management, and antimicrobial choices before and after IDQ and AST results. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.019DOI Listing
March 2019
1 Read

Evaluation of MALDI-TOF MS and an expanded custom reference spectra database for the identification and differentiation of Taylorella equigenitalis and Taylorella asinigenitalis.

Diagn Microbiol Infect Dis 2019 Mar 2. Epub 2019 Mar 2.

ANSES, Nancy laboratory for hydrology, Water Microbiology Unit, MALDI-TOF platform, Nancy, France.

Misidentification between Taylorella equigenitalis, the causative agent of contagious equine metritis (CEM), and Taylorella asinigenitalis is observed by the gold standard culture method. The performance of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) for Taylorella species identification was evaluated using 85 T. equigenitalis and 28 T. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.017DOI Listing
March 2019
3 Reads

Tenofovir Alafenamide and Tenofovir Disoproxil Fumarate are not transported by Concentrative Nucleoside Transporter 2.

Diagn Microbiol Infect Dis 2018 Jul 6. Epub 2018 Jul 6.

Department of Medical Sciences, University of Turin, Amedeo di Savoia Hospital, Turin, Italy. Electronic address:

Tenofovir-associated renal toxicity is influenced by several factors, including plasma exposure and genetic variants in transporter-encoding genes. Tenofovir plasma exposure has been associated with a polymorphism in SLC28A2 gene (encoding the concentrative nucleoside transporter 2, CNT2): particularly, SLC28A2 124 CT/TT genotype patients show higher plasma tenofovir concentrations, compared to CC group. In literature, substrate studies are lacking; for this reason, our aim was to understand if tenofovir and tenofovir-alafenamide are CNT2 substrates. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.07.001DOI Listing

Cost-effectiveness of rapid diagnostic assays that perform directly on blood samples for the diagnosis of septic shock.

Diagn Microbiol Infect Dis 2019 Mar 3. Epub 2019 Mar 3.

Infectious Diseases Division, Warren Alpert Medical School of Brown University, Rhode Island Hospital, Providence, RI, United States. Electronic address:

Molecular diagnostic assays that test directly whole blood provide the ability to decrease inappropriate antimicrobial therapy and improve survival in patients with septic shock. We developed a decision analysis model to evaluate the cost-effectiveness of the addition of molecular assays to blood cultures in adults admitted to medical ICUs with septic shock. Under baseline assumptions, the use of molecular diagnostic methods was cost-saving in all cases that the length of hospital stay differed by 2 and 4 days between patients receiving appropriate and inappropriate antimicrobial therapy. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.018DOI Listing
March 2019
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Antimicrobial treatment challenges in the era of carbapenem resistance.

Diagn Microbiol Infect Dis 2019 Feb 5. Epub 2019 Feb 5.

The University of Queensland Centre for Clinical Research (UQCCR), Royal Brisbane and Women's Hospital, Herston, QLD, Australia; Infectious Diseases, Department of Diagnostics and Public Health, University of Verona, Italy. Electronic address:

Infections due to carbapenem-resistant Gram-negative bacteria are burdened by high mortality and represent an urgent threat to address. Clinicians are currently at a dawn of a new era in which antibiotic resistance in Gram-negative bacilli is being dealt with by the availability of the first new antibiotics in this field for many years. Although new antibiotics have shown promising results in clinical trials, there is still uncertainty over whether their use will improve clinical outcomes in real world practice. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.020DOI Listing
February 2019

Association of systemic antimicrobials with the expression of beta-lactamases in bacteria cultured from urological patients.

Diagn Microbiol Infect Dis 2019 Feb 10. Epub 2019 Feb 10.

Department of Pathology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA. Electronic address:

Background: Patients with abnormalities of the genitourinary tract are at high risk for infections with antimicrobial-resistant pathogens.

Methods: All urine cultures ordered by members of the Division of Urology from four quarterly one-week periods were included. All gram-negative bacilli isolated were analyzed using the Check-Points Check-MDR CT103XL assay to identify the presence of genes associated with resistance to beta-lactam antibiotics. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.007DOI Listing
February 2019

Emergence of colistin resistance during treatment of recurrent pneumonia caused by carbapenemase producing Klebsiella pneumoniae.

Diagn Microbiol Infect Dis 2019 Feb 21. Epub 2019 Feb 21.

Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.

A 60-year-old woman received meropenem/colistin treatment for bilateral pneumonia caused by a ST15 carbapenemase producing Klebsiella pneumoniae. The patient recovered but re-infection with the same (ST15), but now colistin-resistant K. pneumoniae, occurred. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.014DOI Listing
February 2019
1 Read
2.457 Impact Factor

Case report: Identification of intra-laboratory blood culture contamination with Staphylococcus aureus by whole genome sequencing.

Diagn Microbiol Infect Dis 2019 Feb 23. Epub 2019 Feb 23.

South Australia(SA) Pathology, Flinders Medical Centre, Bedford Park, South Australia, 5042, Australia; Department of Microbiology and Infectious Diseases, Flinders Medical Centre, Bedford Park, South Australia, 5042, Australia.

Staphylococcus aureus in blood cultures is rarely considered a contaminant. We report a case of intra-laboratory contamination between blood culture bottles which was confirmed by whole genome sequencing, highlighting the importance of molecular analysis in the clinical laboratory setting. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.016DOI Listing
February 2019
2 Reads

Osmotic stress induces biofilm production by Staphylococcus epidermidis isolates from neonates.

Diagn Microbiol Infect Dis 2019 Feb 20. Epub 2019 Feb 20.

Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address:

Staphylococcus epidermidis is one of the leading causes of bloodstream infections, particularly in premature neonates, and biofilm formation is a major virulence factor. We characterized biofilm formation by 50 S. epidermidis neonatal isolates under osmotic stress and evaluated the expression of biofilm-associated genes. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.009DOI Listing
February 2019

Evaluation of the SHIGA TOXIN QUIK CHEK after overnight enrichment as screening tool for Shiga toxin-producing Escherichia coli detection in human fecal samples.

Diagn Microbiol Infect Dis 2019 Jan 26. Epub 2019 Jan 26.

Reference and Research Laboratory of Food and Waterborne Bacterial Infections, National Center for Microbiology, Institute of Health Carlos III, Majadahonda, Madrid, Spain.

We evaluated the SHIGA TOXIN QUIK CHEK (STQC) on its suitability for Shiga toxin-producing Escherichia coli (STEC) testing on human fecal samples after overnight enrichment. Our in-house PCR-based protocol for STEC detection was used as the standard for comparison. STQC detected all described Shiga toxin subtypes with the only exception of Stx2f. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.010DOI Listing
January 2019

Emergence of a multidrug-resistant clinical isolate of Escherichia coli ST8499 strain producing NDM-13 carbapenemase in the Republic of Korea.

Diagn Microbiol Infect Dis 2019 Feb 21. Epub 2019 Feb 21.

Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Gyeonggi-do, Republic of Korea.

We described a carbapenem-resistant Escherichia coli ST8499 strain producing New Delhi metallo-β-lactamase-13 (NDM-13) from patient in Korea. The isolate exhibited multidrug resistance, but remained susceptible to colistin and tigecycline. The bla gene was located on a 130-kb self-transmissible plasmid. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.013DOI Listing
February 2019

No in vitro synergistic effect of bedaquiline combined with fluoroquinolones, linezolid, and clofazimine against extensively drug-resistant tuberculosis.

Diagn Microbiol Infect Dis 2019 Feb 19. Epub 2019 Feb 19.

Department of Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing, China. Electronic address:

Purpose: We explored the potential synergistic effect of bedaquiline (BDQ) combined with moxifloxacin (MFX), gatifloxacin (GAT), clofazimine (CLO), and linezolid (LZD) for treatment of extensively drug-resistant tuberculosis (XDR-TB).

Methods: Of 191 XDR-TB isolates, 20 exhibiting minimal inhibitory concentration (MIC) values ≥0.063 μg/mL for BDQ were selected to study potential synergistic, additive, or antagonistic drug effects using a checkerboard assay. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.012DOI Listing
February 2019
1 Read
2.457 Impact Factor

Changing serotype distribution and resistance patterns among pediatric nasopharyngeal pneumococci collected in Moscow, 2010-2017.

Diagn Microbiol Infect Dis 2019 Feb 16. Epub 2019 Feb 16.

National Medical Research Center for Children's Health, 119991, Lomonosovskiy prosp., 2, Moscow, Russia.

Serotype distribution and antimicrobial resistance were analyzed in 632 nasopharyngeal pneumococcal isolates collected at a single pediatric center in 2010-2017 before and following the introduction of the 13-valent pneumococcal conjugated vaccine (PCV13) in Russia in 2014. The mean prevalence of PCV13 serotypes was 77.7% in 2010-2015 with a significant decline to 58. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.010DOI Listing
February 2019
2 Reads

Evaluation of heavy metal tolerance genes in plasmids harbored in multidrug-resistant Salmonella enterica and Escherichia coli isolated from poultry in Brazil.

Diagn Microbiol Infect Dis 2019 Feb 1. Epub 2019 Feb 1.

School of Pharmaceutical Sciences of Ribeirão Preto-University of São Paulo (USP), Ribeirão Preto, SP, 14040-903, Brazil. Electronic address:

The emergence and spread of bacteria tolerant to heavy metal ions used in disinfectants have become a new threat to antimicrobial therapies. In this study, metal tolerance genes were analyzed in multidrug-resistant (MDR) Enterobacteriaceae isolated from chickens in Brazil. Different tolerance genes were found disseminated among the MDR isolates. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.019DOI Listing
February 2019

Recurrent Clostridioides (Clostridium) difficile infection in a patient suffering from inflammatory bowel disease and benefits of resistotyping.

Diagn Microbiol Infect Dis 2019 Feb 16. Epub 2019 Feb 16.

Department of Medical Microbiology, Medical University of Sofia, 1431 Sofia, Bulgaria.

Two-year Clostridioides (Clostridium) difficile recurrences in a boy with ulcerative colitis are described. Isolates were toxin A/B positive and nonhypervirulent, and resistotypes of 2017 isolates differed from those in 2016, suggesting a reinfection, later confirmed by multilocus sequence typing (ST49 and ST92, respectively). Resistotypes may show the need of genotypic analysis. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.011DOI Listing
February 2019
2 Reads

Corrigendum to "Influence of GeneXpert MRSA/SA test implementation on clinical outcomes of Staphylococcus aureus bacteremia - A before-after retrospective study" [Diagn Microbiol Infect Dis 2019;93(2):120-124].

Diagn Microbiol Infect Dis 2019 May 8;94(1):107. Epub 2019 Mar 8.

Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel; Infectious Diseases Unit, Rabin Medical Center, Beilinson Hospital, Petach-Tikva, Israel.

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.003DOI Listing

Molecular characterization and pathogenicity determination of hypervirulent Klebsiella pneumoniae clinical isolates serotype K2 in Mexico.

Diagn Microbiol Infect Dis 2019 Jan 31. Epub 2019 Jan 31.

Centro de Investigación Sobre Enfermedades Infecciosas (CISEI), Instituto Nacional de Salud Pública (INSP), Cuernavaca, Morelos, México. Electronic address:

Hypervirulent Klebsiella pneumoniae have been rarely described in Latin America. This work describes the characterization of hypervirulent K. pneumoniae isolates capsular serotype K2 belonging to sequence types 86 and 380. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.013DOI Listing
January 2019

Neonatal bacteremia and oligoarthritis caused by Rhodococcus corynebacterioides/Rhodococcus kroppenstedtii.

Diagn Microbiol Infect Dis 2019 Feb 14. Epub 2019 Feb 14.

Department of Pathology and Laboratory Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, OH.

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.005DOI Listing
February 2019

Corrigendum to "Clinical significance of coryneform Gram-positive rods from blood identified by MALDI-TOF mass spectrometry and their susceptibility profiles - a retrospective chart review" [Diagn Microbiol Infect Dis 2016;85(3):372-376].

Diagn Microbiol Infect Dis 2019 May 8;94(1):106. Epub 2019 Mar 8.

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA; Division of Infectious Diseases, Department of Medicine, Mayo Clinic, Rochester, MN 55905, USA.

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.002DOI Listing

Disseminated "Haemophilus quentini" infection in a patient with multiple myeloma - a case report and review of the literature.

Diagn Microbiol Infect Dis 2019 Feb 10. Epub 2019 Feb 10.

Central Laboratories, Ministry of Health, Jerusalem, Israel.

We describe a case report of a 56-year-old male with undiagnosed multiple myeloma who had severe sepsis associated with pneumonia, meningitis, polyarthritis, and osteomyelitis related to invasive "Haemophilus quentini" infection. The genus was misidentified as H. influenzae by the common bacterial identification systems including newly introduced syndromic PCR-based methods. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.02.004DOI Listing
February 2019
2 Reads

Micafungin prophylaxis in routine medical practice in adult and pediatric patients with hematological malignancy: a prospective, observational study in France.

Diagn Microbiol Infect Dis 2019 Jan 26. Epub 2019 Jan 26.

Hôpitaux Universitaires de Strasbourg and Université de Strasbourg, Inserm, UMR-S1113/IRFAC, Strasbourg, France.

This prospective, observational, multicenter study evaluated the real-world incidence of invasive fungal infection (IFI) during and after micafungin prophylaxis in France. Patients with a hematological malignancy/solid tumor received micafungin prophylaxis according to usual clinical practice and were followed for 3 months. Primary endpoint was breakthrough IFI incidence during prophylaxis. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.011DOI Listing
January 2019
5 Reads
2.457 Impact Factor

Biofilm prevention concentrations (BPC) of minocycline compared to polymyxin B, meropenem, and amikacin against Acinetobacter baumannii.

Diagn Microbiol Infect Dis 2019 Jan 29. Epub 2019 Jan 29.

Infectious Diseases Research Program, Providence Veterans Affairs Medical Center, Providence, RI, United States; College of Pharmacy, University of Rhode Island, Kingston, RI, United States; Center of Innovation in Long-Term Support Services, Providence Veterans Affairs Medical Center, Providence, RI, United States; Warren Alpert Medical School of Brown University, Division of Infectious Diseases, Providence, RI. Electronic address:

Infections caused by Acinetobacter baumannii are difficult to treat as they are often multidrug resistant (MDR) and frequently form biofilms. We investigated the activities of minocycline, polymyxin B, meropenem, and amikacin against diverse Acinetobacter baumannii strains with biofilm formation classified as weak versus moderate/strong. At clinically achievable concentrations, minocycline prevented biofilm formation for 96% of isolates versus 54% for polymyxin B, 29% for meropenem and 29% for amikacin. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.016DOI Listing
January 2019

Community-acquired pneumonia in children: cell-free plasma sequencing for diagnosis and management.

Diagn Microbiol Infect Dis 2019 Feb 2. Epub 2019 Feb 2.

University of California San Diego, Department of Pediatrics, Division of Critical Care.

Community-acquired pneumonia (CAP) is a common cause of pediatric hospital admission. Empiric antibiotic therapy for hospitalized children with serious CAP now targets the most likely pathogen(s), including those that may demonstrate significant antibiotic resistance. Cell-free plasma next-generation sequencing (CFPNGS) was first made available for Pediatric Infectious Diseases physicians in June 1, 2017, to supplement standard-of-care diagnostic techniques. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.016DOI Listing
February 2019
5 Reads

Ceftobiprole activity when tested against contemporary bacteria causing bloodstream infections in the United States (2016-2017).

Diagn Microbiol Infect Dis 2019 Jan 26. Epub 2019 Jan 26.

JMI Laboratories, North Liberty, Iowa, USA.

Ceftobiprole medocaril, the prodrug of ceftobiprole, is an advanced-generation cephalosporin that is approved in many European and non-European countries for the treatment of adults with hospital-acquired pneumonia (excluding ventilator-associated pneumonia) and community-acquired pneumonia and is currently being evaluated in a global phase 3 clinical trial of patients with Staphylococcus aureus bacteremia. This study investigated the in vitro activity of ceftobiprole and comparators against a total of 5466 gram-positive and -negative isolates from bloodstream infections (BSIs) that were collected in the United States during 2016 and 2017 as part of the SENTRY Antimicrobial Surveillance Program. Ceftobiprole was highly active (isolates were >99% susceptible) against S. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.015DOI Listing
January 2019
3 Reads

Evaluation of in vitro activity of ceftolozane-tazobactam compared to other antimicrobial agents against Pseudomonas aeruginosa isolates from cystic fibrosis patients.

Diagn Microbiol Infect Dis 2019 Jan 27. Epub 2019 Jan 27.

Department of Medical, Oral and Biotechnological Sciences; "G. d'Annunzio" University of Chieti, Via Vestini 31, 66100 Chieti, Italy; Center of Excellence on Aging and Translational Medicine (CeSI-MeT), "G. d'Annunzio" University of Chieti, Via Luigi Polacchi 11, 66100 Chieti, Italy. Electronic address:

The in vitro activity of ceftolozane-tazobactam (C-T) was evaluated comparatively to other antibiotics against 188 Pseudomonas aeruginosa isolates collected from cystic fibrosis (CF) patients. Overall, the activity of C-T was comparable to colistin (susceptibility rate: 85.1% vs. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.012DOI Listing
January 2019
1 Read

Comparison of two commercial ELISA kits for detection of rubella specific IgM in suspected congenital rubella syndrome cases and rubella IgG antibodies in a serosurvey of pregnant women.

Diagn Microbiol Infect Dis 2019 Jan 24. Epub 2019 Jan 24.

Diagnostic Virology Group, Indian Council of Medical Research-National Institute of Virology, Pune, India. Electronic address:

Enzyme linked immunosorbent assay (ELISA) for antibody identification, is important for laboratory confirmation of rubella infection in different settings. The Enzygnost rubella ELISA, widely used in the World Health Organization (WHO) Global Measles and Rubella Laboratory Network, is expensive and often unavailable. Qualitative and quantitative performance of the Euroimmun ELISA was compared with the Enzygnost ELISA, for detection of rubella specific IgM, using 283 sera collected from suspected congenital rubella syndrome (CRS) patients and IgG antibodies using 435 sera from a serosurvey among pregnant women. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.009DOI Listing
January 2019
5 Reads

Evaluation of the new Abbott Real-Time EBV assay: fully automated quantification of EBV in whole blood by targeting BLLF1.

Diagn Microbiol Infect Dis 2019 Jan 3. Epub 2019 Jan 3.

Biomedical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea. Electronic address:

The accurate measurement of the Epstein-Barr virus (EBV) DNA level in the blood is required for managing EBV-associated diseases. A new commercial Abbott Real-Time EBV assay, which targets the BLLF1 gene, was evaluated on 120 clinical whole blood samples and the Qnostics EBV analytical panel. The limit of detection of the assay was 48. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.015DOI Listing
January 2019
1 Read

Epidemiology of human respiratory viruses in children with acute respiratory tract infection in a 3-year hospital-based survey in Northern Italy.

Diagn Microbiol Infect Dis 2019 Jan 17. Epub 2019 Jan 17.

Department of Medicine and Surgery, University of Parma, Viale A. Gramsci 14, 43126 Parma, Italy.

Acute respiratory tract infections (ARTIs) are among the leading causes of morbidity and mortality in children. The viral etiology of ARTIs was investigated over 3 years (October 2012-September 2015) in 2575 children in Parma, Italy, using indirect immunofluorescent staining of respiratory samples for viral antigens, cell culture, and molecular assays. Respiratory viruses were detected in 1299 cases (50. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.008DOI Listing
January 2019
3 Reads

Pitfalls in big data analysis: next-generation technologies, last-generation data.

Diagn Microbiol Infect Dis 2018 Dec 15. Epub 2018 Dec 15.

Department of Microbiology, The University of Hong Kong, Hong Kong, China; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong, China. Electronic address:

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.006DOI Listing
December 2018
2 Reads

Establishment of a comprehensive and high throughput serological algorithm for Zika virus diagnostic testing.

Diagn Microbiol Infect Dis 2019 Jan 14. Epub 2019 Jan 14.

Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada. Electronic address:

The previous serological algorithm for Zika virus (ZIKV) comprised screening by anti-ZIKV IgM capture ELISA (MAC-ELISA) for samples collected within 3 months postexposure or onset (MPEO). Samples positive by MAC-ELISA and samples collected beyond 3 MPEO were tested by the confirmatory plaque reduction neutralization test (PRNT), which proved laborious and time-consuming during the 2015 outbreak. Thus, we evaluated several ZIKV ELISAs to establish an anti-IgM and anti-IgG combination for use as a screening tool for all samples prior to PRNT confirmation. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.004DOI Listing
January 2019
5 Reads
2.457 Impact Factor

Evaluation of the Abbott ARCHITECT™ cytomegalovirus IgM/IgG, rubella IgM/IgG, and syphilis treponemal antibodies enzyme immunoassays in a mother and child health center population.

Diagn Microbiol Infect Dis 2019 Jan 17. Epub 2019 Jan 17.

Université de Montréal, 2900 Boulevard Édouard-Montpetit, Montréal, Québec, Canada, H3T 1J4; Centre Hospitalier Universitaire Sainte-Justine, 3175 Chemin de la Côte Sainte-Catherine, Montreal, Quebec, Canada, H3T 1C4.

This study evaluated the concordance of Architect™ chemiluminescent microparticle immunoassays with Captia™ ELISA for cytomegalovirus (CMV) IgM and IgG, with Enzygnost™ and Captia™ ELISA for rubella IgM and IgG and with Trep-Sure™ ELISA for syphilis treponemal antibodies in a mixed pediatric and obstetrical population. Total agreement between assays and Kappa statistic value were 82.5% (95% CI: 75. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07328893193002
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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.017DOI Listing
January 2019
8 Reads

Microbiology of polymicrobial prosthetic joint infection.

Diagn Microbiol Infect Dis 2019 Jan 15. Epub 2019 Jan 15.

Divisions of Clinical Microbiology, Mayo Clinic, Rochester, MN; Infectious Diseases, Mayo Clinic, Rochester, MN. Electronic address:

Prosthetic joint infection (PJI) is a rare but challenging complication of arthroplasty. Herein, we describe the epidemiology and microbiology of PJI, with a focus on analyzing differences between the microbiology of polymicrobial versus monomicrobial infection of hip, knee, and shoulder prostheses. In addition, we report the most frequent co-pathogens in polymicrobial infections, as detected by culture. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.006DOI Listing
January 2019
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Comparison of FTD® respiratory pathogens 33 and a singleplex CDC assay for the detection of respiratory viruses: A study from Cameroon.

Diagn Microbiol Infect Dis 2019 Jan 16. Epub 2019 Jan 16.

Department of Virology, Centre Pasteur of Cameroon, 451 Rue 2005, P.O. Box 1274, Yaoundé, Cameroon. Electronic address:

Introduction: This study compares the detection of 14 common respiratory viruses by two different real-time reverse transcription polymerase chain reaction (rRT-PCR) methods: in house singleplex tests developed by the Centers for Disease Control and Prevention and the commercially available Fast Track Diagnostic (FTD®) Respiratory Pathogens 33 multiplex test.

Methods: A total of 217 nasopharyngeal swabs were analyzed using CDC singleplex rRT-PCR and FTD® Respiratory Pathogens 33 multiplex assays, for the detection of 14 respiratory viruses.

Results: The results showed that 179/217 (82. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.007DOI Listing
January 2019
4 Reads
2.457 Impact Factor

Rapid microarray-based assay for detection of pyrazinamide resistant Mycobacterium tuberculosis.

Diagn Microbiol Infect Dis 2018 Dec 30. Epub 2018 Dec 30.

Alere Technologies GmbH, Jena, Germany.

Pyrazinamide (PZA) is a key antibiotic for the treatment of drug susceptible tuberculosis. PZA-resistance is mainly mediated by mutations in the pncA gene; however the current gold standard is a phenotypic drug susceptibility test requiring a well-adjusted pH-value for reliable results. Our melting curve assay detects a non-wild type genotype in selected pncA regions in at least 3750 gene copies/mL within 2. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.011DOI Listing
December 2018

Efficacy of a 14-day course of amoxicillin for patients with erythema migrans.

Diagn Microbiol Infect Dis 2019 Jan 10. Epub 2019 Jan 10.

Division of Infectious Diseases, New York Medical College, Valhalla, NY 10595.

Although a 14-day treatment course with amoxicillin is in wide clinical usage to treat early Lyme disease, only a few published studies exist to validate its efficacy and safety, with none in the United States. In this study, we reviewed the records of 24 prospectively followed adult patients with erythema migrans who were prescribed a 14-day course of amoxicillin, 500 mg 3 times daily. Treatment with amoxicillin was well tolerated and uniformly successful in resolving the erythema migrans skin lesion and in preventing the development of an objective neurologic, cardiac, or rheumatologic manifestation. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.003DOI Listing
January 2019
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A multisite implementation of a real-time polymerase chain reaction assay to predict ciprofloxacin susceptibility in Neisseria gonorrhoeae.

Diagn Microbiol Infect Dis 2019 Jan 14. Epub 2019 Jan 14.

UCLA Division of Infectious Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.

There are no commercially available Food and Drug Administration-cleared rapid tests for Neisseria gonorrhoeae antimicrobial susceptibility testing. This study evaluated the performance of a laboratory-developed real-time polymerase chain reaction assay for genotyping the gyrA gene to determine antimicrobial susceptibility to ciprofloxacin. Validation and clinical performance of the gyrA assay were evaluated across 3 geographic locations (Los Angeles, San Francisco, Philadelphia). Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.018DOI Listing
January 2019
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Performance of the Accelerate Diagnostics Pheno system with resin-containing BacT/ALERT® Plus blood culture bottles.

Diagn Microbiol Infect Dis 2019 Jan 16. Epub 2019 Jan 16.

Clinical Microbiology Laboratory, Department of Pathology, Duke University Health System. Electronic address:

Bacteremia and septicemia require rapid identification (ID) and antimicrobial susceptibility testing (AST) to start targeted, appropriate therapy. To answer this need, Accelerate Diagnostics, Inc., developed the Accelerate Pheno™ system (AXDX), a fast ID and phenotypic AST platform. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2019.01.002DOI Listing
January 2019
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CHROMagar™ orientation urine culture medium produces matrix-assisted laser desorption ionization-time-of-flight mass spectrometry spectra misidentified as Mycoplasma arginini and Mycoplasma alkalescens.

Diagn Microbiol Infect Dis 2019 Jan 2. Epub 2019 Jan 2.

Shared Health, Diagnostic Services, Clinical Microbiology, Winnipeg, Manitoba, Canada; Department of Medical Microbiology and Infectious Diseases, College of Medicine, University of Manitoba. Winnipeg, Manitoba, Canada.

Matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry is commonly used to identify bacteria and yeasts. Studies indicate that MALDI-TOF is relatively indifferent to the medium used for culture. We report on an investigation into high- and low-confidence MALDI-TOF misidentifications of Mycoplasma arginini and Mycoplasma alkalescens from urine specimens plated to CHROMagar™ Orientation medium that appear to be due to the intrinsic mass spectrum of the medium. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.014DOI Listing
January 2019
1 Read