Metataxonomic approach to decipher the polymicrobial burden in diabetic foot ulcer and its biofilm mode of infection.
- Karthika Suryaletha,
- Joby John,
- Megha Periyappilly Radhakrishnan,
- Sanil George,
- Sabu Thomas
Int Wound J 2018 Jan 22. Epub 2018 Jan 22.
Cholera and Biofilm Research Lab, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala, India.
Chronic diabetic foot is a global burden affecting millions of people, and the chronicity of an ulcer is directly linked to the diverse bacterial burden and its biofilm mode of infection. The bacterial diversity of 100 chronic diabetic ulcer samples was profiled via traditional culturing method as well as metagenomic approach by sequencing the 16S rRNA V3 hyper-variable region on Illumina Miseq Platform (Illumina, Inc., San Diego, CA). Read More