4,272 results match your criteria Developmental Cell [Journal]


Signaling Dynamics Control Cell Fate in the Early Drosophila Embryo.

Dev Cell 2019 Feb;48(3):361-370.e3

Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA. Electronic address:

The Erk mitogen-activated protein kinase plays diverse roles in animal development. Its widespread reuse raises a conundrum: when a single kinase like Erk is activated, how does a developing cell know which fate to adopt? We combine optogenetic control with genetic perturbations to dissect Erk-dependent fates in the early Drosophila embryo. We find that Erk activity is sufficient to "posteriorize" 88% of the embryo, inducing gut endoderm-like gene expression and morphogenetic movements in all cells within this region. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807193000
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http://dx.doi.org/10.1016/j.devcel.2019.01.009DOI Listing
February 2019
1 Read

Microfluidics as an Emerging Precision Tool in Developmental Biology.

Dev Cell 2019 Feb;48(3):293-311

Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany. Electronic address:

Microfluidics has become a precision tool in modern biology. It enables omics data to be obtained from individual cells, as compared to averaged signals from cell populations, and it allows manipulation of biological specimens in entirely new ways. Cells and organisms can be perturbed at extraordinary spatiotemporal resolution, revealing mechanistic insights that would otherwise remain hidden. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.01.015DOI Listing
February 2019

Vitamin E Function in Stress Sensing and Signaling in Plants.

Dev Cell 2019 Feb;48(3):290-292

Department of Evolutionary Biology, Ecology and Environmental Sciences, Faculty of Biology, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain. Electronic address:

Vitamin E is involved in heat stress acclimation. In this issue of Developmental Cell, Fang et al. (2018) uncover a new link between Vitamin E and plant retrograde signaling and show that vitamin E is required for the accumulation of 3'-phosphoadenosine 5'-phosphate, an inhibitor of exoribonucleases, to protect microRNAs from degradation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807193005
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http://dx.doi.org/10.1016/j.devcel.2019.01.023DOI Listing
February 2019
2 Reads

Developmental Erk Signaling Illuminated.

Dev Cell 2019 Feb;48(3):289-290

Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland. Electronic address:

How a small number of signaling pathways can be re-used in distinct embryonic contexts to control different fates remains unclear. In this issue of Developmental Cell, Johnson and Toettcher (2019) use optogenetic approaches to explore how different dynamic ERK signaling states control specific developmental fates in the Drosophila embryo. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.01.022DOI Listing
February 2019
9.708 Impact Factor

Invasion by Force: The C. elegans Anchor Cell Leads the Way.

Dev Cell 2019 Feb;48(3):287-288

CRUK Beatson Institute and Institute of Cancer Sciences, University of Glasgow, G61 1BD, Glasgow, UK. Electronic address:

How cells breach basement membrane barriers remains an area of active research. In this issue of Developmental Cell, Kelley et al. (2019) reveal that the C. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.01.024DOI Listing
February 2019

Aneuploidy in Oocytes Is Prevented by Sustained CDK1 Activity through Degron Masking in Cyclin B1.

Dev Cell 2019 Feb 5. Epub 2019 Feb 5.

Cell Division Biology Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK. Electronic address:

Successful mitosis requires that cyclin B1:CDK1 kinase activity remains high until chromosomes are correctly aligned on the mitotic spindle. It has therefore been unclear why, in mammalian oocyte meiosis, cyclin B1 destruction begins before chromosome alignment is complete. Here, we resolve this paradox and show that mouse oocytes exploit an imbalance in the ratio of cyclin B1 to CDK1 to control CDK1 activity; early cyclin B1 destruction reflects the loss of an excess of non-CDK1-bound cyclin B1 in late prometaphase, while CDK1-bound cyclin B1 is destroyed only during metaphase. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.01.008DOI Listing
February 2019

Live Tracking of Inter-organ Communication by Endogenous Exosomes In Vivo.

Dev Cell 2019 Feb 5. Epub 2019 Feb 5.

Institut Curie, PSL Research University, CNRS UMR144, Paris 75005, France; Institute for Psychiatry and Neuroscience Paris, Hopital Saint-Anne, Université Descartes, INSERM U894, Paris 75014, France. Electronic address:

Extracellular vesicles (EVs) are released by most cell types but providing evidence for their physiological relevance remains challenging due to a lack of appropriate model organisms. Here, we developed an in vivo model to study EV function by expressing CD63-pHluorin in zebrafish embryos. A combination of imaging methods and proteomic analysis allowed us to study biogenesis, composition, transfer, uptake, and fate of individual endogenous EVs. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.01.004DOI Listing
February 2019

Dying Neurons Utilize Innate Immune Signaling to Prime Glia for Phagocytosis during Development.

Dev Cell 2019 Feb 5. Epub 2019 Feb 5.

Department of Neurosciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. Electronic address:

Glia continuously survey neuronal health during development, providing trophic support to healthy neurons while rapidly engulfing dying ones. These diametrically opposed functions necessitate a foolproof mechanism enabling glia to unambiguously identify those neurons to support versus those to engulf. To ensure specificity, glia are proposed to interact with dying neurons via a series of carefully choreographed steps. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.019DOI Listing
February 2019

FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver Tumorigenesis.

Dev Cell 2019 Feb 4. Epub 2019 Feb 4.

State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China; Cancer Research Center of Xiamen University, Xiamen, Fujian 361102, China. Electronic address:

The external factors that modulate Hippo signaling remain elusive. Here, we report that FGF15 activates Hippo signaling to suppress bile acid metabolism, liver overgrowth, and tumorigenesis. FGF15 is induced by FXR in ileal enterocytes in response to increased amounts of intestinal bile. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.021DOI Listing
February 2019

Studying the Fate of Tumor Extracellular Vesicles at High Spatiotemporal Resolution Using the Zebrafish Embryo.

Dev Cell 2019 Feb 4. Epub 2019 Feb 4.

INSERM UMR_S1109, Strasbourg 67200, France; Université de Strasbourg, Strasbourg 67200, France; Fédération de Médecine Translationnelle de Strasbourg, Strasbourg 67200, France. Electronic address:

Tumor extracellular vesicles (EVs) mediate the communication between tumor and stromal cells mostly to the benefit of tumor progression. Notably, tumor EVs travel in the bloodstream, reach distant organs, and locally modify the microenvironment. However, visualizing these events in vivo still faces major hurdles. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.01.014DOI Listing
February 2019

CIPK11-Dependent Phosphorylation Modulates FIT Activity to Promote Arabidopsis Iron Acquisition in Response to Calcium Signaling.

Dev Cell 2019 Jan 25. Epub 2019 Jan 25.

Institute of Botany, Heinrich-Heine University, Düsseldorf 40225, Germany. Electronic address:

Nutrient acquisition is entangled with growth and stress in sessile organisms. The bHLH transcription factor FIT is a key regulator of Arabidopsis iron (Fe) acquisition and post-translationally activated upon low Fe. We identified CBL-INTERACTING PROTEIN KINASE CIPK11 as a FIT interactor. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.01.006DOI Listing
January 2019

Comparative Epigenomics Reveals that RNA Polymerase II Pausing and Chromatin Domain Organization Control Nematode piRNA Biogenesis.

Dev Cell 2019 Jan 30. Epub 2019 Jan 30.

MRC London Institute of Medical Sciences, London W12 0NN, UK; Institute of Clinical Sciences, Imperial College London, London W12 0NN, UK. Electronic address:

Piwi-interacting RNAs (piRNAs) are important for genome regulation across metazoans, but their biogenesis evolves rapidly. In Caenorhabditis elegans, piRNA loci are clustered within two 3-Mb regions on chromosome IV. Each piRNA locus possesses an upstream motif that recruits RNA polymerase II to produce an ∼28 nt primary transcript. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.026DOI Listing
January 2019

Dynamic Interactions of Plant CNGC Subunits and Calmodulins Drive Oscillatory Ca Channel Activities.

Dev Cell 2019 Jan 30. Epub 2019 Jan 30.

Department of Plant and Microbial Biology, University of California at Berkeley, Berkeley, CA 94720, USA. Electronic address:

Calcium is a universal signal in all eukaryotes, but the mechanism for encoding calcium signatures remains largely unknown. Calcium oscillations control pollen tube growth and fertilization in flowering plants, serving as a model for dissecting the molecular machines that mediate calcium fluctuations. We report that pollen-tube-specific cyclic nucleotide-gated channels (CNGC18, CNGC8, and CNGC7) together with calmodulin 2 (CaM2) constitute a molecular switch that either opens or closes the calcium channel depending on cellular calcium levels. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.025DOI Listing
January 2019

Heat Oscillations Driven by the Embryonic Cell Cycle Reveal the Energetic Costs of Signaling.

Dev Cell 2019 Jan 25. Epub 2019 Jan 25.

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.

All living systems function out of equilibrium and exchange energy in the form of heat with their environment. Thus, heat flow can inform on the energetic costs of cellular processes, which are largely unknown. Here, we have repurposed an isothermal calorimeter to measure heat flow between developing zebrafish embryos and the surrounding medium. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.024DOI Listing
January 2019

Vitamin D Stimulates Cardiomyocyte Proliferation and Controls Organ Size and Regeneration in Zebrafish.

Dev Cell 2019 Jan 30. Epub 2019 Jan 30.

Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA; Regeneration Next, Duke University, Durham, NC 27710, USA. Electronic address:

Attaining proper organ size during development and regeneration hinges on the activity of mitogenic factors. Here, we performed a large-scale chemical screen in embryonic zebrafish to identify cardiomyocyte mitogens. Although commonly considered anti-proliferative, vitamin D analogs like alfacalcidol had rapid, potent mitogenic effects on embryonic and adult cardiomyocytes in vivo. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.01.001DOI Listing
January 2019

Population and Single-Cell Analysis of Human Cardiogenesis Reveals Unique LGR5 Ventricular Progenitors in Embryonic Outflow Tract.

Dev Cell 2019 Jan 31. Epub 2019 Jan 31.

Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm 171 77, Sweden; Department of Medicine-Cardiology, Karolinska Institutet, Stockholm 141 86, Sweden. Electronic address:

The morphogenetic process of mammalian cardiac development is complex and highly regulated spatiotemporally by multipotent cardiac stem/progenitor cells (CPCs). Mouse studies have been informative for understanding mammalian cardiogenesis; however, similar insights have been poorly established in humans. Here, we report comprehensive gene expression profiles of human cardiac derivatives from multipotent CPCs to intermediates and mature cardiac cells by population and single-cell RNA-seq using human embryonic stem cell-derived and embryonic/fetal heart-derived cardiac cells micro-dissected from specific heart compartments. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807193000
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http://dx.doi.org/10.1016/j.devcel.2019.01.005DOI Listing
January 2019
3 Reads

Lysosomal Signaling Promotes Longevity by Adjusting Mitochondrial Activity.

Dev Cell 2019 Jan 23. Epub 2019 Jan 23.

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA; Huffington Center on Aging, Baylor College of Medicine, Houston, TX 77030, USA; Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address:

Lysosomes and mitochondria are both crucial cellular organelles for metabolic homeostasis and organism health. However, mechanisms linking their metabolic activities to promote organism longevity remain poorly understood. We discovered that the induction of specific lysosomal signaling mediated by a LIPL-4 lysosomal acid lipase and its lipid chaperone LBP-8 increases mitochondrial ß-oxidation to reduce lipid storage and promote longevity in Caenorhabditis elegans. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807183112
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http://dx.doi.org/10.1016/j.devcel.2018.12.022DOI Listing
January 2019
4 Reads

A Hierarchical Regulatory Landscape during the Multiple Stages of EMT.

Dev Cell 2019 Jan 23. Epub 2019 Jan 23.

Department of Biomedicine, University of Basel, Basel 4058, Switzerland. Electronic address:

Epithelial-mesenchymal transition (EMT) enables cells to gain migratory and invasive features underlined by major transcriptional and epigenetic reprogramming. However, most studies have focused on the endpoints of the EMT process, and the epistatic hierarchy of the transcriptional networks underlying EMT has remained elusive. We have used a siRNA-based, functional high-content microscopy screen to identify 46 (co)transcription factors ((co)TFs) and 13 miRNAs critically required for EMT in normal murine mammary gland (NMuMG) cells. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807183112
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http://dx.doi.org/10.1016/j.devcel.2018.12.023DOI Listing
January 2019
3 Reads

Polarization of Myosin II Refines Tissue Material Properties to Buffer Mechanical Stress.

Dev Cell 2019 Jan;48(2):245-260.e7

MRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK; Institute for the Physics of Living Systems, University College London, London WC1E 6BT, UK; College of Information and Control, Nanjing University of Information Science and Technology, Nanjing, Jiangsu 210044, China. Electronic address:

As tissues develop, they are subjected to a variety of mechanical forces. Some of these forces are instrumental in the development of tissues, while others can result in tissue damage. Despite our extensive understanding of force-guided morphogenesis, we have only a limited understanding of how tissues prevent further morphogenesis once the shape is determined after development. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353629PMC
January 2019
1 Read

Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation.

Dev Cell 2019 Jan;48(2):184-199.e5

Institut Curie, PSL Research University, CNRS UMR 3347, INSERM U1021, Orsay 91405, France; Université Paris Sud, Université Paris-Saclay, CNRS UMR 3347, INSERM U1021, Orsay 91405, France. Electronic address:

During cerebellar development, granule neuron progenitors (GNPs) proliferate by transducing Sonic Hedgehog (SHH) signaling via the primary cilium. Precise regulation of ciliogenesis, thus, ensures proper GNP pool expansion. Here, we report that Atoh1, a transcription factor required for GNPs formation, controls the presence of primary cilia, maintaining GNPs responsiveness to SHH. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.017DOI Listing
January 2019
1 Read

Open Chromatin, Epigenetic Plasticity, and Nuclear Organization in Pluripotency.

Dev Cell 2019 Jan;48(2):135-150

Department of Genetics and Edmond and Lily Center for Brain Sciences (ELSC), Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9190400, Israel. Electronic address:

Pluripotent embryonic stem cells (ESCs) are considered to have open and accessible chromatin relative to differentiated cells. However, as many studies supporting these conclusions relied on ESCs grown in serum, it has been suggested that some of these features are the result of culture conditions, particularly as more recent work using GSK3/MEK inhibitors ("2i") to mimic "ground-state" conditions of the pre-implantation blastocyst observed some altered epigenetic features. Here, we systematically review chromatin and epigenetic features in 2i- and serum-grown conditions to come to a clearer picture of what are genuine characteristics of pluripotency and what open chromatin features predict pluripotency. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.01.003DOI Listing
January 2019

Stored Lipids: More Than Mere Fuel.

Dev Cell 2019 Jan;48(2):133-134

Department of Medicine, Division of Endocrinology, Metabolism, and Diabetes, University of Colorado, Anschutz Medical Campus, Aurora, CO, USA. Electronic address:

Stored lipids fuel early development, but with adulthood comes changing metabolic needs. In this issue of Developmental Cell, Storelli et al. (2018) show that the Drosophila HNF4 nuclear receptor drives adults to convert lipids to very long chain fatty acids and hydrocarbons for an anti-dehydration function likely conserved in mice. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.01.010DOI Listing
January 2019

SUFU: The Jekyll and Hyde of the Cerebellum.

Dev Cell 2019 Jan;48(2):131-132

Division of Regenerative Medicine, Department of Medicine, University of California, San Diego, CA 92037, USA. Electronic address:

Pediatric tumors have enriched the understanding of germline genotype contribution to tumorigenesis. In this issue of Developmental Cell, Yin et al. (2018) describe genetic models of Sonic Hedgehog (SHH) subgroup of medulloblastoma with SUFU alterations, painting more nuanced roles for SUFU in tumorigenesis and maintenance of Gli2 transcription factor circuitries. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.01.013DOI Listing
January 2019
1 Read

Atoh1/MATH1 Adds Up to Ciliogenesis for Transducing SHH Signaling in the Cerebellum.

Dev Cell 2019 Jan;48(2):129-130

Department of Pediatrics, Emory University, Atlanta, GA 303022, USA. Electronic address:

In the developing cerebellum, Sonic hedgehog (SHH) signaling is required for expansion of cerebellar granule neural progenitors, proposed to be cells-of-origin for the SHH-driven pediatric brain tumor medulloblastoma. In this issue of Developmental Cell, Chang et al. (2019) show that the transcription factor Atoh1/MATH1 regulates primary cilium formation, enabling SHH signaling. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.01.012DOI Listing
January 2019
1 Read

Endothelial Cell Killing by TAK1 Inhibition: A Novel Anti-angiogenic Strategy in Cancer Therapy.

Dev Cell 2019 Jan;48(2):127-128

Centre for Cell Death, Cancer and Inflammation, UCL Cancer Institute, University College London, WC1E 6DD London, UK. Electronic address:

TAK1 is a key mediator of proinflammatory signals. In this issue of Developmental Cell, Naito et al. (2019) report that TAK1 loss from endothelial cells in adult mice results in intestinal and hepatic vascular destruction due to TNF-induced death of endothelial cells. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.01.011DOI Listing
January 2019

Adaptive F-Actin Polymerization and Localized ATP Production Drive Basement Membrane Invasion in the Absence of MMPs.

Dev Cell 2019 Feb 24;48(3):313-328.e8. Epub 2019 Jan 24.

Department of Biology, Regeneration Next, Duke University, Box 90338, Durham, NC 27708, USA. Electronic address:

Matrix metalloproteinases (MMPs) are associated with decreased patient prognosis but have failed as anti-invasive drug targets despite promoting cancer cell invasion. Through time-lapse imaging, optical highlighting, and combined genetic removal of the five MMPs expressed during anchor cell (AC) invasion in C. elegans, we find that MMPs hasten invasion by degrading basement membrane (BM). Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372315PMC
February 2019
1 Read

Membrane Curvature and Tension Control the Formation and Collapse of Caveolar Superstructures.

Dev Cell 2019 Jan 14. Epub 2019 Jan 14.

Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel. Electronic address:

Caveolae, flask-shaped pits covered by caveolin-cavin coats, are abundant features of the plasma membrane of many cells. Besides appearing as single-membrane indentations, caveolae are organized as superstructures in the form of rosette-like clusters, whose mechanism of assembly and biological functions have been elusive. Here, we propose that clustering of caveolae in mature muscle cells is driven by forces originating from the elastic energy of membrane-bending deformations and membrane tension. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807183107
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http://dx.doi.org/10.1016/j.devcel.2018.12.005DOI Listing
January 2019
5 Reads

Axon-Dependent Patterning and Maintenance of Somatosensory Dendritic Arbors.

Dev Cell 2019 Jan 17;48(2):229-244.e4. Epub 2019 Jan 17.

Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA. Electronic address:

The mechanisms that pattern and maintain dendritic arbors are key to understanding the principles that govern nervous system assembly. The activity of presynaptic axons has long been known to shape dendrites, but activity-independent functions of axons in this process have remained elusive. Here, we show that in Caenorhabditis elegans, the axons of the ALA neuron control guidance and extension of the 1° dendrites of PVD somatosensory neurons independently of ALA activity. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.015DOI Listing
January 2019
2 Reads

The Lin28/let-7 Pathway Regulates the Mammalian Caudal Body Axis Elongation Program.

Dev Cell 2019 Feb 17;48(3):396-405.e3. Epub 2019 Jan 17.

Division of Pediatric Hematology/Oncology, Children's Hospital Boston, Boston, MA 02115, USA; Howard Hughes Medical Institute, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Harvard Stem Cell Institute, Boston, MA 02115, USA. Electronic address:

The heterochronic genes Lin28a/b and let-7 regulate invertebrate development, but their functions in patterning the mammalian body plan remain unexplored. Here, we describe how Lin28/let-7 influence caudal vertebrae number during body axis formation. We found that FoxD1-driven overexpression of Lin28a strikingly increased caudal vertebrae number and tail bud cell proliferation, whereas its knockout did the opposite. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.016DOI Listing
February 2019
1 Read

Tail Bud Progenitor Activity Relies on a Network Comprising Gdf11, Lin28, and Hox13 Genes.

Dev Cell 2019 Feb 17;48(3):383-395.e8. Epub 2019 Jan 17.

Instituto Gulbenkian de Ciencia, Rua da Quinta Grande 6, 2780-156 Oeiras, Portugal. Electronic address:

During the trunk-to-tail transition, axial progenitors relocate from the epiblast to the tail bud. Here, we show that this process entails a major regulatory switch, bringing tail bud progenitors under Gdf11 signaling control. Gdf11 mutant embryos have an increased number of such progenitors that favor neural differentiation routes, resulting in a dramatic expansion of the neural tube. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.004DOI Listing
February 2019
1 Read

TAK1 Prevents Endothelial Apoptosis and Maintains Vascular Integrity.

Dev Cell 2019 Jan 10;48(2):151-166.e7. Epub 2019 Jan 10.

Department of Signal Transduction, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan; Laboratory of Signal Transduction, World Premier Institute Immunology Frontier Research Center, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan. Electronic address:

TNF-α is a pleiotropic cytokine that has the potential to induce apoptosis under inflammation. How endothelial cells (ECs) are spared from this fate in inflammatory environments where TNF-α is present is not known. Here, we show that TGF-β-activated kinase 1 (TAK1) ensures EC survival and maintains vascular integrity upon TNF-α stimulation. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.002DOI Listing
January 2019
2 Reads

Tumor-Derived Ligands Trigger Tumor Growth and Host Wasting via Differential MEK Activation.

Dev Cell 2019 Jan 10;48(2):277-286.e6. Epub 2019 Jan 10.

Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Howard Hughes Medical Institute, Boston, MA 02115, USA. Electronic address:

Interactions between tumors and host tissues play essential roles in tumor-induced systemic wasting and cancer cachexia, including muscle wasting and lipid loss. However, the pathogenic molecular mechanisms of wasting are still poorly understood. Using a fly model of tumor-induced organ wasting, we observed aberrant MEK activation in both tumors and host tissues of flies bearing gut-yki tumors. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368352PMC
January 2019
4 Reads

TRAPPing a Rab GTPase by the Tail.

Dev Cell 2019 Jan;48(1):9-11

Program in Molecular Medicine and Department of Biochemistry & Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA. Electronic address:

GEFs play a key role in activation and membrane targeting of Rab GTPases. In this issue of Developmental Cell, Thomas et al. (2018) demonstrate how two TRAPP complexes with a common GEF core select distinct Rab substrates through a steric gating mechanism involving their hypervariable tails. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807183107
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http://dx.doi.org/10.1016/j.devcel.2018.12.008DOI Listing
January 2019
3 Reads

FISHing for β Cells.

Dev Cell 2019 Jan;48(1):7-8

Barbara Davis Center for Diabetes, University of Colorado Anschutz Medical Center, Aurora, CO 80045, USA. Electronic address:

β cell heterogeneity has emerged as an important contributor to islet function, with potential implications for diabetes. Using an optimized smFISH technique in intact islets, Farack et al. (2018) identify in the islet core an endocrine cell population of "extreme" β cells with distinct molecular properties. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807183107
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http://dx.doi.org/10.1016/j.devcel.2018.12.007DOI Listing
January 2019
3 Reads

TRIMing Neural Connections with Ubiquitin.

Authors:
Denise J Montell

Dev Cell 2019 Jan;48(1):5-6

Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA 93106, USA. Electronic address:

During evolution, ubiquitin ligases increased in number with increasing nervous system complexity. Recent work shows that proper brain development, cognitive ability, and social behavior in mice require the ubiquitin ligase TRIM67. The work illuminates how general regulators like ubiquitin promote specific functions such as nervous system wiring during development. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.012DOI Listing
January 2019
1 Read

Neurog3-Independent Methylation Is the Earliest Detectable Mark Distinguishing Pancreatic Progenitor Identity.

Dev Cell 2019 Jan;48(1):49-63.e7

Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA; Program in Developmental Biology and Center for Stem Cell Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA. Electronic address:

In the developing pancreas, transient Neurog3-expressing progenitors give rise to four major islet cell types: α, β, δ, and γ; when and how the Neurog3 cells choose cell fate is unknown. Using single-cell RNA-seq, trajectory analysis, and combinatorial lineage tracing, we showed here that the Neurog3 cells co-expressing Myt1 (i.e. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.11.048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327977PMC
January 2019
3 Reads

Microglia: Picky Brain Eaters.

Dev Cell 2019 Jan;48(1):3-4

Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland. Electronic address:

Many aspects of brain development, function, and repair depend on the interaction of neurons with brain immune cells, the microglia. By combining CLEM and SPIM microscopy, a recent study has challenged the current view that microglia can "eat" entire synapses, highlighting the incredible complexity of neuronal-microglial interactions in vivo. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.013DOI Listing
January 2019
1 Read

Membrane Mechanics in Living Cells.

Authors:
Jay T Groves

Dev Cell 2019 Jan;48(1):15-16

Department of Chemistry, University of California, Berkeley, CA, USA. Electronic address:

The combination of fluid and mechanical properties exhibited by cell membranes enables a wealth of physical phenomena, many of which also have biological functions. A recent study in Cell (Shi et al., 2018) now shows that membrane tension in the cell may not be as simple as it first seemed. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.011DOI Listing
January 2019
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Unraveling a Local Inhibitory Mechanism Safeguarding Regular Lateral Root Spacing.

Dev Cell 2019 Jan;48(1):13-14

Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium; VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium. Electronic address:

The mechanisms underlying even spacing of lateral roots remains incompletely understood. In this issue of Developmental Cell, Toyokura et al. make an important step forward by showing how a local inhibitory mechanism involving a novel peptide hormone-receptor cascade acts as a safeguarding mechanism to regulate lateral root spacing. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.010DOI Listing
January 2019
6 Reads

Two Negatives Make a Positive for Insulin Secretion and Growth.

Dev Cell 2019 Jan;48(1):11-12

The Francis Crick Institute, 1 Midland Road, London NW1 1AA, UK. Electronic address:

The confusingly named growth-blocking peptides are nutrient-dependent adipokines that stimulate insulin secretion and boost growth in developing flies. In this issue of Developmental Cell, Meschi et al. (2018) show that these adipose tissue-derived factors regulate insulin secretion by silencing a pair of inhibitory neurons that synapse with insulin-producing cells. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.009DOI Listing
January 2019
2 Reads

An Electric Take on Neural Fate and Cortical Development.

Dev Cell 2019 Jan;48(1):1-2

MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Francis Crick Avenue, Cambridge, UK. Electronic address:

Membrane potential is well-studied in neuronal physiology, but its role in brain development and cell fate determination is less well understood. In two recent studies, bioelectric properties of progenitors and migrating neurons are shown to be tightly regulated, and their disruption leads to abnormalities in fate determination and neuronal positioning. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.12.014DOI Listing
January 2019
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The Atypical MAP Kinase ErkB Transmits Distinct Chemotactic Signals through a Core Signaling Module.

Dev Cell 2018 Dec 31. Epub 2018 Dec 31.

Cell Biology Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK. Electronic address:

Signaling from chemoattractant receptors activates the cytoskeleton of crawling cells for chemotaxis. We show using phosphoproteomics that different chemoattractants cause phosphorylation of the same core set of around 80 proteins in Dictyostelium cells. Strikingly, the majority of these are phosphorylated at an [S/T]PR motif by the atypical MAP kinase ErkB. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807183103
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http://dx.doi.org/10.1016/j.devcel.2018.12.001DOI Listing
December 2018
2 Reads

SPOP Promotes Nanog Destruction to Suppress Stem Cell Traits and Prostate Cancer Progression.

Dev Cell 2019 Feb 27;48(3):329-344.e5. Epub 2018 Dec 27.

Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA. Electronic address:

Frequent SPOP mutation defines the molecular feature underlying one of seven sub-types of human prostate cancer (PrCa). However, it remains largely elusive how SPOP functions as a tumor suppressor in PrCa. Here, we report that SPOP suppresses stem cell traits of both embryonic stem cells and PrCa cells through promoting Nanog poly-ubiquitination and subsequent degradation. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.11.035DOI Listing
February 2019
2 Reads

Dermal Condensate Niche Fate Specification Occurs Prior to Formation and Is Placode Progenitor Dependent.

Dev Cell 2019 Jan 27;48(1):32-48.e5. Epub 2018 Dec 27.

Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, Atran Building AB7-10C, Box 1020, New York, NY 10029, USA; Department of Cell, Developmental, and Regenerative Biology, Icahn School of Medicine at Mount Sinai, Atran Building AB7-10C, Box 1020, New York, NY 10029, USA; Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, Atran Building AB7-10C, Box 1020, New York, NY 10029, USA; Department of Dermatology, Icahn School of Medicine at Mount Sinai, Atran Building AB7-10C, Box 1020, New York, NY 10029, USA. Electronic address:

Cell fate transitions are essential for specification of stem cells and their niches, but the precise timing and sequence of molecular events during embryonic development are largely unknown. Here, we identify, with 3D and 4D microscopy, unclustered precursors of dermal condensates (DC), signaling niches for epithelial progenitors in hair placodes. With population-based and single-cell transcriptomics, we define a molecular time-lapse from pre-DC fate specification through DC niche formation and establish the developmental trajectory as the DC lineage emerges from fibroblasts. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.11.034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370312PMC
January 2019
1 Read

Probing the Function of Metazoan Histones with a Systematic Library of H3 and H4 Mutants.

Dev Cell 2019 Feb 27;48(3):406-419.e5. Epub 2018 Dec 27.

School of Life Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Shanghai 201210, PR China; Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, PR China. Electronic address:

Replication-dependent histone genes often reside in tandemly arrayed gene clusters, hindering systematic loss-of-function analyses. Here, we used CRISPR/Cas9 and the attP/attB double-integration system to alter numbers and sequences of histone genes in their original genomic context in Drosophila melanogaster. As few as 8 copies of the histone gene unit supported embryo development and adult viability, whereas flies with 20 copies were indistinguishable from wild-types. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.11.047DOI Listing
February 2019
1 Read

AMPK Promotes SPOP-Mediated NANOG Degradation to Regulate Prostate Cancer Cell Stemness.

Dev Cell 2019 Feb 27;48(3):345-360.e7. Epub 2018 Dec 27.

Tongji University Cancer Center, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, China. Electronic address:

NANOG is an essential transcriptional factor for the maintenance of embryonic stem cells (ESCs) and cancer stem cells (CSCs) in prostate cancer (PCa). However, the regulation mechanism of NANOG protein stability in cancer progression is still elusive. Here, we report that NANOG is degraded by SPOP, a frequently mutated tumor suppressor of PCa. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.11.033DOI Listing
February 2019
5 Reads
9.708 Impact Factor

Chloroplast-to-Nucleus Signaling Regulates MicroRNA Biogenesis in Arabidopsis.

Dev Cell 2019 Feb 27;48(3):371-382.e4. Epub 2018 Dec 27.

Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Center for Life Sciences, Beijing 100084, China. Electronic address:

As integral regulators in plant development and stress response, microRNAs (miRNAs) themselves need to be tightly regulated. Here, we show that tocopherols (vitamin E), lipid-soluble antioxidants synthesized from tyrosine in chloroplasts, positively regulate the biogenesis of miRNAs. Tocopherols are required for the accumulation of 3'-phosphoadenosine 5'-phosphate (PAP), a retrograde inhibitor of the nuclear exoribonucleases (XRN), which may protect primary miRNAs from being degraded and promote mature miRNA production. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.11.046DOI Listing
February 2019
1 Read
9.708 Impact Factor

Single-Cell Analysis Reveals a Hair Follicle Dermal Niche Molecular Differentiation Trajectory that Begins Prior to Morphogenesis.

Dev Cell 2019 Jan 27;48(1):17-31.e6. Epub 2018 Dec 27.

Department of Dermatology, Yale University, New Haven, CT 06520, USA; Department of Pathology, Yale University, New Haven, CT 06520, USA; Yale Cancer Center, New Haven, CT 06520, USA; Yale Stem Cell Center, New Haven, CT 06520, USA. Electronic address:

Delineating molecular and cellular events that precede appendage morphogenesis has been challenging due to the inability to distinguish quantitative molecular differences between cells that lack histological distinction. The hair follicle (HF) dermal condensate (DC) is a cluster of cells critical for HF development and regeneration. Events that presage emergence of this distinctive population are poorly understood. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.11.032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361530PMC
January 2019
1 Read

Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation.

Dev Cell 2019 Jan 20;48(1):64-75.e5. Epub 2018 Dec 20.

Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai, Kobe 657-8501, Japan. Electronic address:

In plants, the position of lateral roots (LRs) depends on initiation sites induced by auxin. The domain of high auxin response responsible for LR initiation stretches over several cells, but only a pair of pericycle cells (LR founder cells) will develop into LRs. In this work, we identified a signaling cascade controlling LR formation through lateral inhibition. Read More

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http://dx.doi.org/10.1016/j.devcel.2018.11.031DOI Listing
January 2019
2 Reads