4,337 results match your criteria Developmental Cell [Journal]


The Alternative Splicing Regulator Nova2 Constrains Vascular Erk Signaling to Limit Specification of the Lymphatic Lineage.

Dev Cell 2019 Apr;49(2):279-292.e5

Division of Genomics of Development and Disease, Institute for Molecular Bioscience, The University of Queensland, St Lucia, Brisbane, QLD 4073, Australia. Electronic address:

The correct assignment of cell fate within fields of multipotent progenitors is essential for accurate tissue diversification. The first lymphatic vessels arise from pre-existing veins after venous endothelial cells become specified as lymphatic progenitors. Prox1 specifies lymphatic fate and labels these progenitors; however, the mechanisms restricting Prox1 expression and limiting the progenitor pool remain unknown. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.017DOI Listing

Efficient Front-Rear Coupling in Neutrophil Chemotaxis by Dynamic Myosin II Localization.

Dev Cell 2019 Apr;49(2):189-205.e6

Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA; Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address:

Efficient chemotaxis requires rapid coordination between different parts of the cell in response to changing directional cues. Here, we investigate the mechanism of front-rear coordination in chemotactic neutrophils. We find that changes in the protrusion rate at the cell front are instantaneously coupled to changes in retraction at the cell rear, while myosin II accumulation at the rear exhibits a reproducible 9-15-s lag. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.025DOI Listing
April 2019
2 Reads

The Nuclear Arsenal of Cilia.

Dev Cell 2019 Apr;49(2):161-170

Bateson Centre and The Department of Biomedical Science, The University of Sheffield, Sheffield S10 2TN, UK.

Several recent studies have revealed that nuclei and cilia share molecular components implicated in DNA damage response, splicing, gene expression, and sub-compartmentalization of the cell. We review evidence that exchange of components between the nucleus and cilia is facilitated by the centrosome, which contributes both to the mitotic apparatus of the nucleus and to the cilia structure. Moreover, the centrosome and the pericentriolar material form condensates that share components with stress granules and P-bodies, membrane-less organelles enriched in RNA and RNA-processing proteins. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.009DOI Listing

Meiotic Spindle Has a Soft Spot.

Dev Cell 2019 Apr;49(2):159-160

Division of Molecular Biology, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia. Electronic address:

The spindle relies on forces exerted by microtubules and motor proteins to align and segregate chromosomes. In this issue of Developmental Cell, Takagi et al. (2019) show that meiotic spindle microtubules respond differently to forces at different spindle locations, depending on microtubule organization and motor proteins that crosslink them. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.04.004DOI Listing

A PBX/MEIS Complex Balances Reproduction and Somatic Resilience.

Dev Cell 2019 Apr;49(2):157-158

Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA. Electronic address:

Resource reallocation to metabolic processes promoting reproduction is critical for the survival of a species and therefore is tightly regulated. In this issue of Developmental Cell, Dowen (2019) finds that a PBX/MEIS homeodomain transcription factor complex controls a transcriptional network that balances reproduction versus longevity and somatic maintenance. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.04.005DOI Listing

Rap1b Promotes Notch-Signal-Mediated Hematopoietic Stem Cell Development by Enhancing Integrin-Mediated Cell Adhesion.

Dev Cell 2019 Apr 9. Epub 2019 Apr 9.

Department of Molecular Pathophysiology, Institute of Advanced Medical Sciences, Nippon Medical School, 1-396 Kosugi-machi, Nakahara-ku, Kawasaki, Kanagawa 211-8533, Japan. Electronic address:

Hematopoietic stem cells (HSCs) emerge from hemogenic endothelium (HE) within the ventral portion of the dorsal aorta during vertebrate development. In zebrafish, Notch signaling induces HE specification from posterior lateral plate mesoderm (PLPM) cells as they migrate over the ventral surface of the somite. During migration, PLPM cells make close contact with Notch-ligand-expressing somitic cells to acquire HE identity. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.023DOI Listing

An SH3PX1-Dependent Endocytosis-Autophagy Network Restrains Intestinal Stem Cell Proliferation by Counteracting EGFR-ERK Signaling.

Dev Cell 2019 Apr 12. Epub 2019 Apr 12.

Huntsman Cancer Institute and Department of Oncological Sciences, University of Utah, Salt Lake City, UT 84112, USA. Electronic address:

The effect of intracellular vesicle trafficking on stem-cell behavior is largely unexplored. We screened the Drosophila sorting nexins (SNXs) and discovered that one, SH3PX1, profoundly affects gut homeostasis and lifespan. SH3PX1 restrains intestinal stem cell (ISC) division through an endocytosis-autophagy network that includes Dynamin, Rab5, Rab7, Atg1, 5, 6, 7, 8a, 9, 12, 16, and Syx17. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.029DOI Listing

Spectraplakin Shot Maintains Perinuclear Microtubule Organization in Drosophila Polyploid Cells.

Dev Cell 2019 Apr 12. Epub 2019 Apr 12.

School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China. Electronic address:

Polyploid cells endoreplicate their DNA through a modified cell cycle that skips mitosis as part of their differentiation programs. Upon cell-cycle exit and differentiation, non-centrosomal sites govern microtubule distribution in most cells. Little is known on how polyploid cells, differentiated but cycling, organize their microtubules. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.027DOI Listing

An Ectoderm-Derived Myeloid-like Cell Population Functions as Antigen Transporters for Langerhans Cells in Zebrafish Epidermis.

Dev Cell 2019 Apr 15. Epub 2019 Apr 15.

Division of Life Science, State Key Laboratory of Molecular Neuroscience and Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, P.R. China; Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University-Hong Kong University of Science and Technology Medical Center, Shenzhen 518036, P.R. China. Electronic address:

Tissue-resident macrophages (TRMs) are highly heterogeneous and engage in a wide range of diverse functions. Yet, the heterogeneities of their origins and functions remain incompletely defined. Here, we report the identification and characterization of an ectoderm-derived myeloid-like cell, which we refer to as metaphocyte. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.028DOI Listing

Inter-Organ Growth Coordination Is Mediated by the Xrp1-Dilp8 Axis in Drosophila.

Dev Cell 2019 Apr 16. Epub 2019 Apr 16.

Institut Curie, PSL Research University, CNRS UMR3215, INSERM U934, UPMC Paris-Sorbonne, 26 Rue d'Ulm, 75005 Paris, France. Electronic address:

How organs scale with other body parts is not mechanistically understood. We have addressed this question using the Drosophila imaginal disc model. When the growth of one disc domain is perturbed, other parts of the disc and other discs slow down their growth, maintaining proper inter-disc and intra-disc proportions. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.016DOI Listing

Cerebrovascular Injuries Induce Lymphatic Invasion into Brain Parenchyma to Guide Vascular Regeneration in Zebrafish.

Dev Cell 2019 Apr 8. Epub 2019 Apr 8.

Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Molecular Developmental Biology, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China. Electronic address:

Damage to regional cerebrovascular networks and neuronal tissues occurs during acute cerebrovascular diseases, such as ischemic stroke. The promotion of vascular regeneration is the most promising therapeutic approach. To understand the cellular and molecular mechanisms underlying brain vascular regeneration, we developed two zebrafish cerebrovascular injury models using genetic ablation and photochemical thrombosis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807193023
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http://dx.doi.org/10.1016/j.devcel.2019.03.022DOI Listing
April 2019
1 Read

NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy.

Dev Cell 2019 Apr 3. Epub 2019 Apr 3.

Department of Molecular Medicine, Institute of Basic Medical Sciences and Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, 1112 Blindern, Oslo 0317, Norway. Electronic address:

The clearance of damaged or dysfunctional mitochondria by selective autophagy (mitophagy) is important for cellular homeostasis and prevention of disease. Our understanding of the mitochondrial signals that trigger their recognition and targeting by mitophagy is limited. Here, we show that the mitochondrial matrix proteins 4-Nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 (NIPSNAP1) and NIPSNAP2 accumulate on the mitochondria surface upon mitochondrial depolarization. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.013DOI Listing

Oncogenic Notch Triggers Neoplastic Tumorigenesis in a Transition-Zone-like Tissue Microenvironment.

Dev Cell 2019 Apr 4. Epub 2019 Apr 4.

Department of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA. Electronic address:

During the initial stages of tumorigenesis, the tissue microenvironment where the pro-tumor cells reside plays a crucial role in determining the fate of these cells. Transition zones, where two types of epithelial cells meet, are high-risk sites for carcinogenesis, but the underlying mechanism remains largely unclear. Here, we show that persistent upregulation of Notch signaling induces neoplastic tumorigenesis in a transition zone between the salivary gland imaginal ring cells and the giant cells in Drosophila larvae. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.015DOI Listing

Mechanically Distinct Microtubule Arrays Determine the Length and Force Response of the Meiotic Spindle.

Dev Cell 2019 Apr 11;49(2):267-278.e5. Epub 2019 Apr 11.

Center for Frontier Research, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540, Japan; Department of Genetics, School of Life Science, SOKENDAI University, Yata 1111, Mishima, Shizuoka 411-8540, Japan. Electronic address:

The microtubule-based spindle is subjected to various mechanical forces during cell division. How the structure generates and responds to forces while maintaining overall integrity is unknown because we have a poor understanding of the relationship between filament architecture and mechanics. Here, to fill this gap, we combine microneedle-based quantitative micromanipulation with high-resolution imaging, simultaneously analyzing forces and local filament motility in the Xenopus meiotic spindle. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.014DOI Listing

Macropinocytosis Overcomes Directional Bias in Dendritic Cells Due to Hydraulic Resistance and Facilitates Space Exploration.

Dev Cell 2019 Apr 11;49(2):171-188.e5. Epub 2019 Apr 11.

INSERM U932, Institut Curie, ANR-10--IDEX-0001-02 PSL(∗) and ANR-11-LABX-0043, Paris, France. Electronic address:

The migration of immune cells can be guided by physical cues imposed by the environment, such as geometry, rigidity, or hydraulic resistance (HR). Neutrophils preferentially follow paths of least HR in vitro, a phenomenon known as barotaxis. The mechanisms and physiological relevance of barotaxis remain unclear. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807193023
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http://dx.doi.org/10.1016/j.devcel.2019.03.024DOI Listing
April 2019
5 Reads

MIR205HG Is a Long Noncoding RNA that Regulates Growth Hormone and Prolactin Production in the Anterior Pituitary.

Dev Cell 2019 Apr 3. Epub 2019 Apr 3.

Department of Immunology University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA; Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA; Department of Pediatrics, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA. Electronic address:

MicroRNAs (miRNAs) are processed from primary miRNA transcripts (pri-miRNAs), many of which are annotated as long noncoding RNAs (lncRNAs). We assessed whether MIR205HG, the host gene for miR-205, has independent functions as an lncRNA. Comparing mice with targeted deletions of MIR205HG and miR-205 revealed a functional role for the lncRNA in the anterior pituitary. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.012DOI Listing

Loss of Sirtuin 1 Alters the Secretome of Breast Cancer Cells by Impairing Lysosomal Integrity.

Dev Cell 2019 Apr 1. Epub 2019 Apr 1.

Department of Molecular Medicine, Cornell University, Ithaca, NY 14853, USA.

The NAD-dependent deacetylase Sirtuin 1 (SIRT1) is down-regulated in triple-negative breast cancer. To determine the mechanistic basis by which reduced SIRT1 expression influences processes related to certain aggressive cancers, we examined the consequences of depleting breast cancer cells of SIRT1. We discovered that reducing SIRT1 levels decreased the expression of one particular subunit of the vacuolar-type H+ ATPase (V-ATPase), which is responsible for proper lysosomal acidification and protein degradation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807193018
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http://dx.doi.org/10.1016/j.devcel.2019.03.011DOI Listing
April 2019
12 Reads

The Bacterivore's Solution: Fight and Flight to Promote Survival.

Dev Cell 2019 Apr;49(1):7-9

Integrative Program in Molecular and Biomedical Sciences, Baylor College of Medicine, Houston, TX 77030, USA; Huffington Center on Aging, Baylor College of Medicine, Houston, TX 77030, USA; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA; Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address:

Bacterial avoidance and innate immune response are two ways by which C. elegans respond to pathogenic bacteria. In this issue of Developmental Cell, Kumar et al. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.021DOI Listing

Relax, Kinetochores Are Exquisitely Sensitive to Tension.

Dev Cell 2019 Apr;49(1):5-7

Department of Physiology & Biophysics, University of Washington School of Medicine, Seattle, WA 98195, USA. Electronic address:

By estimating the absolute levels of tension at kinetochores in dividing yeast cells and relating these measurements to kinetochore detachment probability, Mukherjee et al. (2019) quantify in this issue of Developmental Cell the force sensitivity of the mitotic error correction system. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.019DOI Listing

The Multiple Ways Nuclei Scale on a Multinucleated Muscle Cell Scale.

Dev Cell 2019 Apr;49(1):3-5

The Laboratory of Biochemistry and Genetics, The National Institute of Diabetes and Digestive and Kidney Diseases, The National Institutes of Health, Bethesda, MD 20892, USA. Electronic address:

In mononucleated cells, nuclear size scales with cell size, but does this relationship extend to multinucleated cells? In this issue of Developmental Cell,Windner et al. (2019) examine scaling of nuclei in multinucleated Drosophila muscle fibers and identify global and local cellular inputs that contribute to nuclear size regulation. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.018DOI Listing

Lifespan Extension in C. elegans Caused by Bacterial Colonization of the Intestine and Subsequent Activation of an Innate Immune Response.

Dev Cell 2019 Apr;49(1):100-117.e6

Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address:

Mechanisms that control aging are important yet poorly defined. To discover longevity control genes, we performed a forward genetic screen for delayed reproductive aging in C. elegans. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.010DOI Listing
April 2019
9.708 Impact Factor

Sorting Out Fate Determination.

Dev Cell 2019 Apr;49(1):1-3

Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, Neuherberg, Germany; German Center for Diabetes Research (DZD), Neuherberg, Germany; Technical University of Munich, Medical Faculty, Munich, Germany. Electronic address:

How organ morphogenesis specifies cell fate and whether organ progenitors are predetermined or specified via niche signals are critical developmental biology questions. In this issue of Developmental Cell, Nyeng et al. (2019) modulate cell-cell adhesion in the pancreas and provide evidence that progenitors are plastic and instructed by niche signals. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.020DOI Listing

CEH-60/PBX and UNC-62/MEIS Coordinate a Metabolic Switch that Supports Reproduction in C. elegans.

Authors:
Robert H Dowen

Dev Cell 2019 Apr 4;49(2):235-250.e7. Epub 2019 Apr 4.

Integrative Program for Biological and Genome Sciences, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Electronic address:

The molecular basis of how animals integrate metabolic, developmental, and environmental information before committing resources to reproduction is an unresolved issue in developmental biology. In C. elegans, adult animals reallocate fat stores from intestinal cells to the germline via low-density lipoprotein (LDL)-like particles to promote embryogenesis. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.002DOI Listing
April 2019
2 Reads

The Long Non-Coding RNA lep-5 Promotes the Juvenile-to-Adult Transition by Destabilizing LIN-28.

Dev Cell 2019 Apr 2. Epub 2019 Apr 2.

Departments of Biomedical Genetics and Neuroscience, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA. Electronic address:

Biological roles for most long non-coding RNAs (lncRNAs) remain mysterious. Here, using forward genetics, we identify lep-5, a lncRNA acting in the C. elegans heterochronic (developmental timing) pathway. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807193018
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http://dx.doi.org/10.1016/j.devcel.2019.03.003DOI Listing
April 2019
1 Read

Leukocyte Cytoskeleton Polarization Is Initiated by Plasma Membrane Curvature from Cell Attachment.

Dev Cell 2019 Apr 28;49(2):206-219.e7. Epub 2019 Mar 28.

Department of Pharmacology, Vascular Biology and Therapeutic Program, Yale University, New Haven, CT 06520, USA. Electronic address:

Cell polarization is important for various biological processes. However, its regulation, particularly initiation, is incompletely understood. Here, we investigated mechanisms by which neutrophils break their symmetry and initiate their cytoskeleton polarization from an apolar state in circulation for their extravasation during inflammation. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.023DOI Listing
April 2019
2 Reads

The Pediatric Cell Atlas: Defining the Growth Phase of Human Development at Single-Cell Resolution.

Dev Cell 2019 Apr 28;49(1):10-29. Epub 2019 Mar 28.

Department of Biomedical Informatics, University of Cincinnati College of Medicine, and Cincinnati Children's Hospital Medical Center, Division of Biomedical Informatics, Cincinnati, OH 45229, USA.

Single-cell gene expression analyses of mammalian tissues have uncovered profound stage-specific molecular regulatory phenomena that have changed the understanding of unique cell types and signaling pathways critical for lineage determination, morphogenesis, and growth. We discuss here the case for a Pediatric Cell Atlas as part of the Human Cell Atlas consortium to provide single-cell profiles and spatial characterization of gene expression across human tissues and organs. Such data will complement adult and developmentally focused HCA projects to provide a rich cytogenomic framework for understanding not only pediatric health and disease but also environmental and genetic impacts across the human lifespan. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.001DOI Listing
April 2019
3 Reads

Spatiotemporal Developmental Trajectories in the Arabidopsis Root Revealed Using High-Throughput Single-Cell RNA Sequencing.

Dev Cell 2019 Mar;48(6):840-852.e5

Center for Plant Molecular Biology, University of Tübingen, Auf der Morgenstelle 32, Tübingen 72076, Germany. Electronic address:

High-throughput single-cell RNA sequencing (scRNA-seq) is becoming a cornerstone of developmental research, providing unprecedented power in understanding dynamic processes. Here, we present a high-resolution scRNA-seq expression atlas of the Arabidopsis root composed of thousands of independently profiled cells. This atlas provides detailed spatiotemporal information, identifying defining expression features for all major cell types, including the scarce cells of the quiescent center. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807193014
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http://dx.doi.org/10.1016/j.devcel.2019.02.022DOI Listing
March 2019
34 Reads

Understanding the Mechanobiology of Early Mammalian Development through Bioengineered Models.

Dev Cell 2019 Mar;48(6):751-763

Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences (SV) and School of Engineering (STI), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland; Institute of Chemical Sciences and Engineering, School of Basic Science (SB), EPFL, Lausanne, Switzerland. Electronic address:

Research in developmental biology has been recently enriched by a multitude of in vitro models recapitulating key milestones of mammalian embryogenesis. These models obviate the challenge posed by the inaccessibility of implanted embryos, multiply experimental opportunities, and favor approaches traditionally associated with organoids and tissue engineering. Here, we provide a perspective on how these models can be applied to study the mechano-geometrical contributions to early mammalian development, which still escape direct verification in species that develop in utero. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.024DOI Listing

TAZ Forces Lateral Inhibition.

Dev Cell 2019 Mar;48(6):748-750

Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA. Electronic address:

New mechanisms by which cells communicate to specify cell fate during animal development are continuously being discovered. In a recently published article in Cell, Xia et al. (2019) identify the transcriptional regulator TAZ as a novel mediator of lateral inhibition in zebrafish oogenesis that directs cell fate through mechanical cues. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.006DOI Listing

Repurposing Kinetochore Microtubule Attachment Machinery in Neurodevelopment.

Dev Cell 2019 Mar;48(6):746-748

Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA. Electronic address:

Kinetochore-microtubule attachments are essential to direct proper chromosome segregation during cell division. In this issue of Developmental Cell, Cheerambathur et al. (2019) and Zhao et al. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.004DOI Listing

Still Searching for Specialized Ribosomes.

Dev Cell 2019 Mar;48(6):744-746

Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA. Electronic address:

Reporting in Developmental Cell, Cenik et al. (2019) show that the maternal ribosome supply is sufficient for C. elegans embryonic development, arguing against tissue-specific specialized ribosomes in this context. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.005DOI Listing

Kidneys Prefer a High Fat4 Diet.

Dev Cell 2019 Mar;48(6):743-744

Departments of Urology, Genetics and Development, and Pathology, Columbia University, 1130 St. Nicholas Avenue, New York, NY 10032, USA. Electronic address:

Ectopic or supernumerary ureteric bud (UB) branches can result in urinary tract obstruction. In this issue of Developmental Cell, Zhang et al. (2019) show that Ret and Fat4, which are expressed on the surface of the UB and surrounding stromal cells, respectively, interact directly to restrict branching during UB outgrowth. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.008DOI Listing
March 2019
1 Read
9.708 Impact Factor

Prometheus Unbound in Ya(p) Heart.

Dev Cell 2019 Mar;48(6):741-742

Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address:

The adult mammalian heart, unlike in some amphibian and fish species, is generally considered a post-mitotic organ. In this issue of Developmental Cell, Monroe et al. (2019) show that the expression of constitutively active YAP induces a remarkable degree of proliferation in preexisting adult cardiomyocytes by globally altering chromatin accessibility. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.007DOI Listing

An Intrinsically Disordered Region in OSBP Acts as an Entropic Barrier to Control Protein Dynamics and Orientation at Membrane Contact Sites.

Dev Cell 2019 Apr 21;49(2):220-234.e8. Epub 2019 Mar 21.

CNRS, Université Côte d'Azur, Institut de Pharmacologie Moléculaire et Cellulaire, 660 route des lucioles, Valbonne 06560, France. Electronic address:

Lipid transfer proteins (LTPs) acting at membrane contact sites (MCS) between the ER and other organelles contain domains involved in heterotypic (e.g., ER to Golgi) membrane tethering as well as domains involved in lipid transfer. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.021DOI Listing

Nuclear Scaling Is Coordinated among Individual Nuclei in Multinucleated Muscle Fibers.

Dev Cell 2019 Apr 21;49(1):48-62.e3. Epub 2019 Mar 21.

Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. Electronic address:

Optimal cell performance depends on cell size and the appropriate relative size, i.e., scaling, of the nucleus. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6464369PMC

Dorsal-Ventral Differences in Neural Stem Cell Quiescence Are Induced by p57/Dacapo.

Dev Cell 2019 Apr 21;49(2):293-300.e3. Epub 2019 Mar 21.

The Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK. Electronic address:

Quiescent neural stem cells (NSCs) in the adult brain are regenerative cells that could be activated therapeutically to repair damage. It is becoming apparent that quiescent NSCs exhibit heterogeneity in their propensity for activation and in the progeny that they generate. We discovered recently that NSCs undergo quiescence in either G or G in the Drosophila brain, challenging the notion that all quiescent stem cells are G arrested. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.015DOI Listing
April 2019
2 Reads

GRASP55 and UPR Control Interleukin-1β Aggregation and Secretion.

Dev Cell 2019 Apr 14;49(1):145-155.e4. Epub 2019 Mar 14.

Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Dr. Aiguader 88, Barcelona 08003, Spain; Universitat Pompeu Fabra (UPF), Barcelona, Spain; Institució Catalana de Recerca i Estudis Avançats (ICREA), Pg. Lluis Companys 23, Barcelona 08010, Spain. Electronic address:

Signal-sequence-lacking interleukin (IL)-1β, is cleaved by caspase-1 to mature mIL-1β, which is secreted, without entering the endoplasmic reticulum. We report that macrophages of GRASP55 mice are defective in mIL-1β secretion and retain it as intracellular aggregates. Intriguingly, GRASP55 macrophages are defective in the IRE1α branch of the unfolded protein response. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.011DOI Listing

Clearance by Microglia Depends on Packaging of Phagosomes into a Unique Cellular Compartment.

Dev Cell 2019 Apr 14;49(1):77-88.e7. Epub 2019 Mar 14.

European Molecular Biology Laboratory (EMBL), Developmental Biology Unit, Heidelberg, Germany; University of Zurich, Institute of Molecular Life Sciences (IMLS), Zurich, Switzerland. Electronic address:

Phagocytic immune cells such as microglia can engulf and process pathogens and dying cells with high efficiency while still maintaining their dynamic behavior and morphology. Effective intracellular processing of ingested cells is likely to be crucial for microglial function, but the underlying cellular mechanisms are poorly understood. Using both living fish embryos and mammalian macrophages, we show that processing depends on the shrinkage and packaging of phagosomes into a unique cellular compartment, the gastrosome, with distinct molecular and ultra-structural characteristics. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.014DOI Listing

Maturation and Clearance of Autophagosomes in Neurons Depends on a Specific Cysteine Protease Isoform, ATG-4.2.

Dev Cell 2019 Apr 14;49(2):251-266.e8. Epub 2019 Mar 14.

Department of Neuroscience, Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA; Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA; Instituto de Neurobiología, Recinto de Ciencias Médicas, Universidad de Puerto Rico, 201 Blvd del Valle, San Juan 00901, Puerto Rico. Electronic address:

In neurons, defects in autophagosome clearance have been associated with neurodegenerative disease. Yet, the mechanisms that coordinate trafficking and clearance of synaptic autophagosomes are poorly understood. Here, we use genetic screens and in vivo imaging in single neurons of C. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.013DOI Listing

The SOS2-SCaBP8 Complex Generates and Fine-Tunes an AtANN4-Dependent Calcium Signature under Salt Stress.

Dev Cell 2019 Mar;48(5):697-709.e5

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address:

Calcium signals act as universal second messengers that trigger many cellular processes in animals and plants, but how specific calcium signals are generated is not well understood. In this study, we determined that AtANN4, a putative calcium-permeable transporter, and its interacting proteins, SCaBP8 and SOS2, generate a calcium signal under salt stress, which initially activates the SOS pathway, a conserved mechanism that modulates ion homeostasis in plants under salt stress. After activation, SCaBP8 promotes the interaction of protein kinase SOS2 with AtANN4, which enhances its phosphorylation by SOS2. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.010DOI Listing
March 2019
46 Reads

Aurora-A Breaks Symmetry in Contractile Actomyosin Networks Independently of Its Role in Centrosome Maturation.

Dev Cell 2019 Mar;48(5):631-645.e6

Temasek Life-Sciences Laboratory, Singapore 117604, Republic of Singapore; Department of Biological Sciences, National University of Singapore, Singapore 117583, Republic of Singapore; Mechanobiology Institute, National University of Singapore, Singapore 117411, Republic of Singapore. Electronic address:

Cell polarity is facilitated by a rearrangement of the actin cytoskeleton at the cell cortex. The program triggering the asymmetric remodeling of contractile actomyosin networks remains poorly understood. Here, we show that polarization of Caenorhabditis elegans zygotes is established through sequential downregulation of cortical actomyosin networks by the mitotic kinase, Aurora-A. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.012DOI Listing

Tackling Plant Phosphate Starvation by the Roots.

Dev Cell 2019 Mar;48(5):599-615

Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, Ghent 9052, Belgium; VIB Center for Plant Systems Biology, Technologiepark 71, Ghent 9052, Belgium. Electronic address:

Plant responses to phosphate deprivation encompass a wide range of strategies, varying from altering root system architecture, entering symbiotic interactions to excreting root exudates for phosphorous release, and recycling of internal phosphate. These processes are tightly controlled by a complex network of proteins that are specifically upregulated upon phosphate starvation. Although the different effects of phosphate starvation have been intensely studied, the full extent of its contribution to altered root system architecture remains unclear. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807193000
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http://dx.doi.org/10.1016/j.devcel.2019.01.002DOI Listing
March 2019
9 Reads

A New Player in Tissue Mechanics: MicroRNA Control of Mechanical Homeostasis.

Dev Cell 2019 Mar;48(5):596-598

Cell and Developmental Biology Programme, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona 08003, Spain; Universitat Pompeu Fabra (UPF), Barcelona 08003, Spain. Electronic address:

How the homeostasis of tissue mechanics is controlled remains an open question. In a recent issue of Nature Cell Biology, Moro et al. (2019) reveal a novel role for miRNAs in regulating mechanotransduction in cells, tissues, and wound healing. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.019DOI Listing

Embryonic Immune Cells Remodel the Heart.

Dev Cell 2019 Mar;48(5):595-596

Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92037, USA; Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92037, USA. Electronic address:

How the products of transient hematopoiesis in the yolk sac, dorsal aorta, and developing heart tube function at their sites of production is poorly understood. In this issue of Developmental Cell, Shigeta et al. (2019) elegantly demonstrate that macrophages derived from the heart tube contribute to local tissue remodeling during valve development. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807193014
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http://dx.doi.org/10.1016/j.devcel.2019.02.017DOI Listing
March 2019
4 Reads

Breaking Symmetry: Worm Cue Finally Found.

Dev Cell 2019 Mar;48(5):593-594

Department of Zoology, Life Sciences Institute, University of British Columbia, Vancouver, BC V6T1Z3, Canada.

In this issue of Developmental Cell, Zhao et al. (2019) show that the Aurora A kinase AIR-1 is the long-sought cue that downregulates cortical actomyosin to establish anterior-posterior polarity in the C. elegans zygote, diffusing from centrosomes to the overlying cortex to phosphorylate yet to be identified target(s). Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.018DOI Listing
March 2019
2 Reads

Measuring the Energetic Costs of Embryonic Development.

Dev Cell 2019 Mar;48(5):591-592

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA; Department of Physics, California Institute of Technology, Pasadena, CA 91125, USA. Electronic address:

What are the thermodynamic costs of development? In this issue of Developmental Cell, Rodenfels et al. (2019) demonstrate that the high energetic cost of coordinated cell division that is regulated by phospho-signaling gives rise to a measurable periodicity in the heat dissipated during zebrafish embryogenesis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15345807193013
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http://dx.doi.org/10.1016/j.devcel.2019.02.016DOI Listing
March 2019
3 Reads

FAT4 Fine-Tunes Kidney Development by Regulating RET Signaling.

Dev Cell 2019 Mar 7;48(6):780-792.e4. Epub 2019 Mar 7.

Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON M5G 1X5, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address:

FAT4 mutations lead to several human diseases that disrupt the normal development of the kidney. However, the underlying mechanism remains elusive. In studying the duplex kidney phenotypes observed upon deletion of Fat4 in mice, we have uncovered an interaction between the atypical cadherin FAT4 and RET, a tyrosine kinase receptor essential for kidney development. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.004DOI Listing
March 2019
1 Read

p120ctn-Mediated Organ Patterning Precedes and Determines Pancreatic Progenitor Fate.

Dev Cell 2019 Apr 7;49(1):31-47.e9. Epub 2019 Mar 7.

Novo Nordisk Foundation Center for Stem Cell Biology (Danstem), University of Copenhagen, 2200 Copenhagen N, Denmark; Institute of Translational Stem Cell Research, Helmholtz Zentrum München, Neuherberg, Germany. Electronic address:

The mechanism of how organ shape emerges and specifies cell fate is not understood. Pancreatic duct and endocrine lineages arise in a spatially distinct domain from the acinar lineage. Whether these lineages are pre-determined or settle once these niches have been established remains unknown. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.005DOI Listing
April 2019
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Kinetochore Proteins Have a Post-Mitotic Function in Neurodevelopment.

Dev Cell 2019 Mar 28;48(6):873-882.e4. Epub 2019 Feb 28.

F.M. Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA 02115, USA; Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA. Electronic address:

The kinetochore is a complex of proteins, broadly conserved from yeast to man, that resides at the centromere and is essential for chromosome segregation in dividing cells. There are no known functions of the core complex outside of the centromere. We now show that the proteins of the kinetochore have an essential post-mitotic function in neurodevelopment. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.003DOI Listing

The Kinetochore-Microtubule Coupling Machinery Is Repurposed in Sensory Nervous System Morphogenesis.

Dev Cell 2019 Mar 28;48(6):864-872.e7. Epub 2019 Feb 28.

Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, CA 92093, USA; Department of Cellular & Molecular Medicine, University of California, San Diego, San Diego, La Jolla, CA 92093, USA. Electronic address:

Dynamic coupling of microtubule ends to kinetochores, built on the centromeres of chromosomes, directs chromosome segregation during cell division. Here, we report that the evolutionarily ancient kinetochore-microtubule coupling machine, the KMN (Knl1/Mis12/Ndc80-complex) network, plays a critical role in neuronal morphogenesis. We show that the KMN network concentrates in microtubule-rich dendrites of developing sensory neurons that collectively extend in a multicellular morphogenetic event that occurs during C. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436928PMC