20,247 results match your criteria Dengue


Insights from direct studies on human dengue infections.

Authors:
Scott B Halstead

Proc Natl Acad Sci U S A 2018 Dec 13. Epub 2018 Dec 13.

Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814

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December 2018

Recent Advances in AIV Biosensors Composed of Nanobio Hybrid Material.

Micromachines (Basel) 2018 Dec 9;9(12). Epub 2018 Dec 9.

School of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea.

Since the beginning of the 2000s, globalization has accelerated because of the development of transportation systems that allow for human and material exchanges throughout the world. However, this globalization has brought with it the rise of various pathogenic viral agents, such as Middle East respiratory syndrome coronavirus (MERS-CoV), severe acute respiratory syndrome coronavirus (SARS-CoV), Zika virus, and Dengue virus. In particular, avian influenza virus (AIV) is highly infectious and causes economic, health, ethnical, and social problems to human beings, which has necessitated the development of an ultrasensitive and selective rapid-detection system of AIV. Read More

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December 2018

The Multifaceted Roles of Autophagy in Flavivirus-Host Interactions.

Authors:
Po-Yuan Ke

Int J Mol Sci 2018 Dec 7;19(12). Epub 2018 Dec 7.

Department of Biochemistry & Molecular Biology and Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan.

Autophagy is an evolutionarily conserved cellular process in which intracellular components are eliminated via lysosomal degradation to supply nutrients for organelle biogenesis and metabolic homeostasis. Flavivirus infections underlie multiple human diseases and thus exert an immense burden on public health worldwide. Mounting evidence indicates that host autophagy is subverted to modulate the life cycles of flaviviruses, such as hepatitis C virus, dengue virus, Japanese encephalitis virus, West Nile virus and Zika virus. Read More

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December 2018

Emerging Mosquito-Borne Threats and the Response from European and Eastern Mediterranean Countries.

Int J Environ Res Public Health 2018 Dec 7;15(12). Epub 2018 Dec 7.

UMR BIPAR, Animal Health Laboratory, ANSES, INRA, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, 94700 Maisons-Alfort, France.

Mosquito-borne viruses are the cause of some of the greatest burdens to human health worldwide, particularly in tropical regions where both human populations and mosquito numbers are abundant. Due to a combination of anthropogenic change, including the effects on global climate and wildlife migration there is strong evidence that temperate regions are undergoing repeated introduction of mosquito-borne viruses and the re-emergence of viruses that previously were not detected by surveillance. In Europe, the repeated introductions of West Nile and Usutu viruses have been associated with bird migration from Africa, whereas the autochthonous transmission of chikungunya and dengue viruses has been driven by a combination of invasive mosquitoes and rapid transcontinental travel by infected humans. Read More

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December 2018

Periods of high dengue transmission defined by rainfall do not impact efficacy of dengue vaccine in regions of endemic disease.

PLoS One 2018 13;13(12):e0207878. Epub 2018 Dec 13.

Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America.

Objective: To evaluate the association of rainy season with overall dengue disease incidence and with the efficacy of the Sanofi Pasteur recombinant, live, attenuated, tetravalent vaccine (CYD-TDV) in two randomized, controlled multicenter phase III clinical trials in Asia and Latin America.

Methods: Rainy seasons were defined for each study site using climatological information from the World Meteorological Organization. The dengue attack rate in the placebo group for each study month was calculated as the number of symptomatic, virologically-confirmed dengue events in a given month divided by the number of participants at risk in the same month. Read More

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December 2018

Evaluation of a Rapid Immunochromatographic Assay and Two Enzyme-Linked Immunosorbent Assays for Detection of IgM-class Antibodies to Zika Virus.

J Clin Microbiol 2018 Dec 12. Epub 2018 Dec 12.

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN

There are currently five serologic assays available for detection of anti-Zika virus (ZIKV) IgM-class antibodies with United States Food and Drug Administration Emergency Use Authorization. Among these are the Chembio DDP Zika IgM system (Chembio DPP Zika ICA; Medford, NY), a rapid immunochromatographic assay (ICA), and the InBios ZIKV ™ 2.0 IgM antibody capture enzyme-linked immunosorbent assay (InBios ZIKV 2. Read More

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December 2018

Comprehensive evaluation of differential serodiagnosis between Zika and dengue viral infection.

J Clin Microbiol 2018 Dec 12. Epub 2018 Dec 12.

US Department of Health and Human Services, Public Health Services, Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80521

Diagnostic testing for Zika virus (ZIKV) or dengue virus (DENV) infection can be accomplished by a nucleic acid detection method; however, a negative result does not exclude infection due to the low virus titer during infection depending on the timing of sample collection. Therefore, a ZIKV- or DENV-specific serological assay is essential for the accurate diagnosis of patients and to mitigate potential severe health outcomes. A retrospective study design with dual approaches of collecting human serum samples for testing was developed. Read More

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December 2018

Data and tools to integrate climate and environmental information into public health.

Infect Dis Poverty 2018 Nov 29;7(1):126. Epub 2018 Nov 29.

The International Research Institute for Climate and Society, The Earth Institute, Columbia University, 61 Route 9W, Lamont-Doherty, Palisades, NY, 10964, USA.

Background: During the last 30 years, the development of geographical information systems and satellites for Earth observation has made important progress in the monitoring of the weather, climate, environmental and anthropogenic factors that influence the reduction or the reemergence of vector-borne diseases. Analyses resulting from the combination of geographical information systems (GIS) and remote sensing have improved knowledge of climatic, environmental, and biodiversity factors influencing vector-borne diseases (VBDs) such as malaria, visceral leishmaniasis, dengue, Rift Valley fever, schistosomiasis, Chagas disease and leptospirosis. These knowledge and products developed using remotely sensed data helped and continue to help decision makers to better allocate limited resources in the fight against VBDs. Read More

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November 2018

Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus.

BMC Biotechnol 2018 Dec 12;18(1):78. Epub 2018 Dec 12.

Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, Centro de Biotecnologia, Av. Prof. Lineu Prestes, 2242, São Paulo, 05508-000, Brazil.

Background: Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these secondary structures. Removal of the chaotropes frequently causes reaggregation and poor recovery of bioactive proteins. Read More

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December 2018

Dengue fever in Dar es Salaam, Tanzania: clinical features and outcome in populations of black and non-black racial category.

BMC Infect Dis 2018 Dec 12;18(1):644. Epub 2018 Dec 12.

Swiss Tropical and Public Health Institute, Basel, Switzerland.

Background: Although the incidence of dengue across Africa is high, severe dengue is reported infrequently. We describe the clinical features and the outcome of dengue according to raceduring an outbreak in Dar es Salaam, Tanzania that occurred in both native and expatriate populations.

Methods: Adults with confirmed dengue (NS1 and/or IgM on rapid diagnostic test and/or PCR positive) were included between December 2013 and July 2014 in outpatient clinics. Read More

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December 2018

Importance of cerebrospinal fluid investigation during dengue infection in Brazilian Amazonia Region.

Mem Inst Oswaldo Cruz 2018 Dec 10;114:e180450. Epub 2018 Dec 10.

Universidade Federal do Estado do Rio de Janeiro/Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.

BACKGROUND Amazon, the largest tropical forest of the world, has suffered from dengue outbreaks since 1998. Cerebrospinal fluid (CSF) of patients, from Amazonas state, suspected of central nervous system (CNS) viral infection was studied using molecular and immunological methods. OBJECTIVE To evaluate the importance of CSF investigation in patients with acute dengue virus (DENV) infection of CNS. Read More

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December 2018

ERRATUM: Spatial analysis of probable cases of dengue fever, chikungunya fever and zika virus infections in Maranhao State, Brazil.

Authors:

Rev Inst Med Trop Sao Paulo 2018 Dec 10;60:e62err. Epub 2018 Dec 10.

[This corrects the article doi: 10.1590/S1678-9946201860062]. Read More

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December 2018

Highlights on the Application of Genomics and Bioinformatics in the Fight Against Infectious Diseases: Challenges and Opportunities in Africa.

Front Genet 2018 27;9:575. Epub 2018 Nov 27.

West African Centre for Cell Biology of Infectious Pathogens, University of Ghana, Accra, Ghana.

Genomics and bioinformatics are increasingly contributing to our understanding of infectious diseases caused by bacterial pathogens such as and parasites such as . This ranges from investigations of disease outbreaks and pathogenesis, host and pathogen genomic variation, and host immune evasion mechanisms to identification of potential diagnostic markers and vaccine targets. High throughput genomics data generated from pathogens and animal models can be combined with host genomics and patients' health records to give advice on treatment options as well as potential drug and vaccine interactions. Read More

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November 2018

The β subunit of Ca1.2 channels is required for an optimal interferon response in cardiac muscle cells.

Sci Signal 2018 Dec 11;11(560). Epub 2018 Dec 11.

Departamento de Farmacología, Centro de Investigación y de Estudios Avanzados del IPN, Ciudad de México, México.

The auxiliary β subunit of the cardiac Ca1.2 channel plays a poorly understood role in gene transcription. Here, we characterized the regulatory effects of the β subunit in H9c2 rat cardiac cells on the abundances of mRNA [which encodes interferon-β (IFN-β)] and of the IFN-β-related genes , , , , , and , as well as on the abundances of the antiviral proteins DDX58, IRF7, STAT2, and IFITM3. Read More

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December 2018

[Prevalence of dengue and leptospira in febrile patients of the general hospital of specialities in Mexico].

Rev Chilena Infectol 2018 ;35(3):332-333

Centro de Investigaciones Biomédicas, Universidad Autónoma de Campeche, Campeche, México.

Among the infectious diseases characterized by a febrile picture are: dengue, leptospirosis, rickettsiosis and salmonellosis, among others. The objective of this study was to identify IgM antibodies against dengue and Leptospira in febrile patientes. The seropositivity for IgM antibodies to dengue was 34%; 26. Read More

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January 2018
1 Read

(Trichocomaceae): A Natural, Eco-Friendly Mycoinsecticide for Control of Malaria, Filariasis, Dengue Vectors and Its Toxicity Assessment Against an Aquatic Model Organism .

Front Pharmacol 2018 26;9:1355. Epub 2018 Nov 26.

Natural Drug Research Laboratory, Department of Biotechnology, School of Biosciences, Periyar University, Salem, India.

Vector-borne diseases like malaria, filariasis, and dengue are transmitted by mosquitoes and they cause global mortality and morbidity due to an increased resistance against commercial insecticides. The present study was aimed to evaluate the neurobehavioral toxicity, knock-down effect, histopathology, ovicidal, adulticidal, and smoke toxicity effect of extract against three mosquito species, namely , and (Diptera: Culicidae). The isolated fungal strain was identified as (GenBank accession no: KX694148. Read More

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November 2018

First Reported Complete Genome Sequence of a Dengue Virus Serotype 4 Strain from Papua New Guinea.

Microbiol Resour Announc 2018 Sep 27;7(12). Epub 2018 Sep 27.

Public Health Virology Laboratory, Forensic and Scientific Services, Coopers Plains, Queensland, Australia.

A male patient in his 50s who traveled from Papua New Guinea (PNG) to Australia in 2016 was diagnosed with a dengue virus serotype 4 (DENV-4) infection, and the virus was isolated from his acute-phase serum. Here, we describe the first complete genome sequence of a DENV-4 strain from PNG. Read More

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September 2018

Comparison of Landmark- and Outline-Based Geometric Morphometrics for Discriminating Mosquito Vectors in Ratchaburi Province, Thailand.

Biomed Res Int 2018 6;2018:6170502. Epub 2018 Nov 6.

College of Allied Health Science, Suan Sunandha Rajabhat University, Samut Songkhram 75000, Thailand.

It is often challenging to identify mosquito vectors in the field based on morphological features due to their similar morphologies and difficulties in obtaining undamaged samples but is required for their successful control. Geometric morphometrics (GM) overcomes this issue by analyzing a suite of traits simultaneously and has the added advantages of being easy to use, low cost, and quick. Therefore, this research compared the efficiency and precision of landmark- and outline-based GM techniques for separating species of mosquitoes in Huay Nam Nak village, Ratchaburi Province, Thailand. Read More

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November 2018

Correlates of Climate Variability and Dengue Fever in Two Metropolitan Cities in Bangladesh.

Cureus 2018 Oct 1;10(10):e3398. Epub 2018 Oct 1.

Epidemiology and Public Health, Griffith University, Brisbane, AUS.

Dengue fever is a major public health concern in Bangladesh with increased incidence during monsoon. We aimed to assess the correlation of temperature, humidity, and rainfall on dengue fever in two dengue endemic cities in Bangladesh. It was a time series analysis of climate factors and dengue occurrence data in Dhaka and Chittagong cities from 1 January 2000 to 31 December 2009. Read More

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October 2018

Dengue outbreaks in Hawai'i After WWII - A Review of Public Health Response and Scientific Literature.

Hawaii J Med Public Health 2018 Dec;77(12):315-318

Department of Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawai'i at Manoa, Honolulu, HI (WYT, WKW).

The four serotypes of dengue virus (DENV) cause the most important and common arthropod-borne viral diseases in humans. There have been three major dengue outbreaks in Hawai'i since 1946. The most recent and largest outbreak occurred on Hawai'i Island in 2015-2016. Read More

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December 2018

Epidemiological profile of dengue in Zhejiang Province, southeast China.

PLoS One 2018 11;13(12):e0208810. Epub 2018 Dec 11.

Zhejiang Provincial Centre for Disease Control and Prevention, Hangzhou, China.

Background: Dengue is one of the most important vector-bore infectious diseases in China because of its drastic increase in incidence, geographic extension and profound influence on China's economy. This study aims to retrospectively uncover the epidemiological profile of dengue in Zhejiang, one of the most developed provinces in China, and to find the problem existing in dengue control and prevention.

Methodology: Descriptive analyses on the dengue incidence and associated factors were performed. Read More

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December 2018

Simultaneous Occurrence of Axonal Guillain-Barré Syndrome in Two Siblings Following Dengue Infection.

Ann Indian Acad Neurol 2018 Oct-Dec;21(4):315-317

Department of Neurology, King George's Medical University, Lucknow, Uttar Pradesh, India.

Guillain-Barré syndrome (GBS) is an infrequent neurological complication of dengue viral infection. It is broadly divided into either acute inflammatory demyelinating variety or an axonal variety (acute motor axonal and acute sensory and motor axonal variants). Axonal variants are distinctly infrequent. Read More

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December 2018

New tetrahydroquinoline and indoline compounds containing a hydroxy cyclopentenone, virantmycin B and C, produced by Streptomyces sp. AM-2504.

J Antibiot (Tokyo) 2018 Dec 10. Epub 2018 Dec 10.

Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.

Two new antibiotics, designated virantmycin B (1) and C (2), were isolated from the cultured broth of Streptomyces sp. AM-2504. Compounds 1 and 2 were purified by Diaion HP-20, silica gel, and octadecylsilane chromatography, followed by high-performance liquid chromatography. Read More

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December 2018

Role of NS1 antibodies in the pathogenesis of acute secondary dengue infection.

Nat Commun 2018 12 7;9(1):5242. Epub 2018 Dec 7.

Centre for Dengue Research, University of Sri Jayewardenepura, Nugegoda, 10100, Sri Lanka.

The role of NS1-specific antibodies in the pathogenesis of dengue virus infection is poorly understood. Here we investigate the immunoglobulin responses of patients with dengue fever (DF) and dengue hemorrhagic fever (DHF) to NS1. Antibody responses to recombinant-NS1 are assessed in serum samples throughout illness of patients with acute secondary DENV1 and DENV2 infection by ELISA. Read More

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December 2018

Virus-inclusive single-cell RNA sequencing reveals the molecular signature of progression to severe dengue.

Proc Natl Acad Sci U S A 2018 Dec 7. Epub 2018 Dec 7.

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305;

Dengue virus (DENV) infection can result in severe complications. However, the understanding of the molecular correlates of severity is limited, partly due to difficulties in defining the peripheral blood mononuclear cells (PBMCs) that contain DENV RNA in vivo. Accordingly, there are currently no biomarkers predictive of progression to severe dengue (SD). Read More

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December 2018

Cyclin G-associated kinase (GAK) affinity and antiviral activity studies of a series of 3-C-substituted isothiazolo[4,3-b]pyridines.

Eur J Med Chem 2018 Nov 28;163:256-265. Epub 2018 Nov 28.

Medicinal Chemistry, Rega Institute for Medical Research, KU Leuven, Herestraat 49, bus 1041, 3000, Leuven, Belgium. Electronic address:

Cyclin G-associated kinase (GAK) is a cellular regulator of the clathrin-associated host adaptor proteins AP-1 and AP-2, which regulates intracellular trafficking of dengue virus during early and late stages of the viral lifecycle. Previously, the discovery of isothiazolo[4,3-b]pyridines as potent and selective GAK inhibitors with promising antiviral activity was reported. In this manuscript, the synthesis of isothiazolo[4,3-b]pyridines with a carbon-linked substituent at position 3 is described by the application of regioselective Suzuki and Sonogashira coupling reactions. Read More

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November 2018

Inhibition of dengue virus by curcuminoids.

Antiviral Res 2018 Dec 6. Epub 2018 Dec 6.

Department of Microbiology and Immunology, Georgetown University, Washington, D.C, USA. Electronic address:

The dengue virus is considered to be a globally important human pathogen prevalent in tropical and subtropical regions of the world. According to a recent estimate, the disease burden due to DENV infections is ∼390 million infections per year globally in ∼100 countries including the southern US, Puerto Rico and Hawaii, resulting in nearly ∼25,000 deaths mostly among children. Despite the significant morbidity and mortality that results from DENV infections, there is currently no effective chemotherapeutic treatment for DENV infections. Read More

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December 2018

Isolation and molecular characterization of Dengue virus clinical isolates from pediatric patients in New Delhi.

Int J Infect Dis 2018 Dec 7. Epub 2018 Dec 7.

Translational Health Science and Technology Institute, Faridabad, Haryana, INDIA. Electronic address:

Objectives: To characterize the in vitro replication fitness, viral diversity and phylogeny of dengue viruses (DENV) isolated from Indian patients.

Methods: DENV was isolated from whole blood collected from patients by passaging in cell culture. Passage 3 viruses were used for growth kinetics in C6/36 mosquito cells. Read More

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December 2018
2.330 Impact Factor

Molecular characterization of the viral structural gene of the first dengue virus type 1 outbreak in Xishuangbanna, a border area of China, Burma and Laos.

Int J Infect Dis 2018 Dec 5. Epub 2018 Dec 5.

Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, PR China; Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming, PR China; Yunnan Key Laboratory of Vector-borne Infectious Disease, Kunming, PR China. Electronic address:

Background: Xishuangbanna, a border area of China, Burma and Laos, had its first major DENV-1 outbreak in 2017. This study aims to explore the genetic characterization, potential source and evolution of the viruses in outbreak.

Methods: The structural protein C/prM/E genes of viruses isolated from local residents or Burmese travelers were sequenced followed by mutation, phylogenetic, homologous recombination, molecular clock and demographic reconstruction analysis. Read More

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December 2018

Bloodstream infections in hospitalized adults with dengue fever: Clinical characteristics and recommended empirical therapy.

J Microbiol Immunol Infect 2018 Nov 23. Epub 2018 Nov 23.

Department of Internal Medicine, National Cheng Kung University Hospital, Tainan, Taiwan; Centre for Infection Control, National Cheng Kung University Hospital, Tainan, Taiwan; Department of Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan. Electronic address:

Background: Dengue is an important mosquito-borne tropical viral disease and dual infection, though rare, has been regarded as a risk factor for severe disease and mortality. However, few studies focused on bloodstream infections (BSIs) and empirical antibiotic therapy rarely addressed.

Methods: Dengue patients with concurrent or subsequent BSIs between July 1 and December 31, 2015 were included. Read More

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November 2018

Platelet factor 4 promotes rapid replication and propagation of Dengue and Japanese encephalitis viruses.

EBioMedicine 2018 Dec 5. Epub 2018 Dec 5.

Disease Biology Laboratory, Regional Centre for Biotechnology, National Capital Region Biotech Science Cluster, Faridabad, India. Electronic address:

Background: Activated platelets release cytokines/proteins including CXCL4 (PF4), CCL5 and fibrinopeptides, which regulate infection of several pathogenic viruses such as HIV, H1N1 and HCV in human. Since platelet activation is the hallmark of Dengue virus (DV) infection, we investigated the role of platelets in DV replication and also in a closely related Japanese Encephalitis virus (JEV).

Methods And Findings: Microscopy and PCR analysis revealed a 4-fold increase in DV replication in primary monocytes or monocytic THP-1 cells in vitro upon incubation with either DV-activated platelets or supernatant from DV-activated platelets. Read More

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December 2018

Cross-reactive dengue virus-derived monoclonal antibodies to Zika virus envelope protein: Panacea or Pandora's box?

BMC Infect Dis 2018 Dec 10;18(1):641. Epub 2018 Dec 10.

Oxford University Hospitals, John Radcliffe Hospital, Oxford, OX3 9DU, UK.

Background: Dengue Virus (DENV) and Zika Virus (ZIKV) are closely related flaviviruses, circulating in overlapping geographical regions. The recent ZIKV epidemic has been linked to an explosion in reports of microcephaly and neurological defects. It is conceivable that our knowledge of DENV might potentiate the development of a ZIKV vaccine due to the close phylogenetic relationship between these flaviviruses and cross-reactive antibodies, principally to the envelope protein (E protein). Read More

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December 2018

A potent prolyl tRNA synthetase inhibitor antagonizes Chikungunya and Dengue viruses.

Antiviral Res 2018 Dec 3;161:163-168. Epub 2018 Dec 3.

Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA; Howard Hughes Medical Institute, Chevy Chase, MD, USA.

Arboviruses represent a group of pathogens that can spread efficiently throughout human populations by hematophagous arthropod vectors. The mosquito-borne (re)emerging Chikungunya and Dengue viruses belong to the alphavirus and flavivirus genus, respectively, with no approved therapeutics or safe vaccines for humans. Transmitted by the same vector Aedes spp. Read More

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December 2018
1 Read

Risk prediction system for dengue transmission based on high resolution weather data.

PLoS One 2018 6;13(12):e0208203. Epub 2018 Dec 6.

IBM Research Australia, Southgate, Victoria, Australia.

Background: Dengue is the fastest spreading vector-borne viral disease, resulting in an estimated 390 million infections annually. Precise prediction of many attributes related to dengue is still a challenge due to the complex dynamics of the disease. Important attributes to predict include: the risk of and risk factors for an infection; infection severity; and the timing and magnitude of outbreaks. Read More

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December 2018
3 Reads

Integrated Aedes management for the control of Aedes-borne diseases.

PLoS Negl Trop Dis 2018 Dec 6;12(12):e0006845. Epub 2018 Dec 6.

MIVEGEC, IRD, CNRS, University of Montpellier, Montpellier, France.

Background: Diseases caused by Aedes-borne viruses, such as dengue, Zika, chikungunya, and yellow fever, are emerging and reemerging globally. The causes are multifactorial and include global trade, international travel, urbanisation, water storage practices, lack of resources for intervention, and an inadequate evidence base for the public health impact of Aedes control tools. National authorities need comprehensive evidence-based guidance on how and when to implement Aedes control measures tailored to local entomological and epidemiological conditions. Read More

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December 2018
6 Reads

Statistical modeling of the effect of rainfall flushing on dengue transmission in Singapore.

PLoS Negl Trop Dis 2018 Dec 6;12(12):e0006935. Epub 2018 Dec 6.

Draper, Cambridge, Massachusetts, United States of America.

Background: Rainfall patterns are one of the main drivers of dengue transmission as mosquitoes require standing water to reproduce. However, excess rainfall can be disruptive to the Aedes reproductive cycle by "flushing out" aquatic stages from breeding sites. We developed models to predict the occurrence of such "flushing" events from rainfall data and to evaluate the effect of flushing on dengue outbreak risk in Singapore between 2000 and 2016. Read More

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December 2018
1 Read

Building the evidence base for dengue vector control: searching for certainty in an uncertain world.

Pathog Glob Health 2018 Dec 6:1-9. Epub 2018 Dec 6.

a Heidelberg Institute of Global Health , University of Heidelberg , Germany.

This review discusses biological and chemical methods for dengue vector control, using recently emerging summary evidence, meta-analyses and systematic reviews to conclude on practical public health recommendations for Aedes control, which is increasingly relevant in an era of widespread Chikungunya, yellow feer and Zika outbreaks. The analysis follows an a priori framework of systematic reviews by the authors on vector control methods, distinguishing vector control methods into biological, chemical and environmental methods. Findings of each published systematic review by the authors, following each individual vector control method, are summarised and compared in the discussion against the findings of existing meta-analyses covering all vector control methods. Read More

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December 2018
1 Read

Dengue type 1 viruses circulating in humans are highly infectious and poorly neutralized by human antibodies.

Proc Natl Acad Sci U S A 2018 Dec 5. Epub 2018 Dec 5.

Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, NC 27599;

The four dengue virus (DENV) serotypes are mosquito-borne flaviviruses of humans. The interactions between DENVs and the human host that lead to asymptomatic, mild, or severe disease are poorly understood, in part, because laboratory models are poor surrogates for human DENV disease. Virologists are interested in how the properties of DENVs replicating in people compare with virions propagated on laboratory cell lines, which are widely used for research and vaccine development. Read More

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December 2018

Q63, a novel DENV2 RdRp non-nucleoside inhibitor, inhibited DENV2 replication and infection.

J Pharmacol Sci 2018 Jun 25. Epub 2018 Jun 25.

State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, PR China. Electronic address:

Dengue virus (DENV) annually infects 400 million people worldwide. Unfortunately, there is lack of widely protective vaccine or drugs against DENV. The viral RNA-dependent RNA polymerase (RdRp) of NS5 protein is highly conserved among different DENV subtypes, thus presenting itself as an attractive target for drug design. Read More

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June 2018
2.360 Impact Factor

The effect of freeze-dried Carica papaya leaf juice treatment on NS1 and viremia levels in dengue fever mice model.

BMC Complement Altern Med 2018 Dec 5;18(1):320. Epub 2018 Dec 5.

Herbal Medicine Research Center, Institute for Medical Research, Kuala Lumpur, Malaysia.

Background: Carica papaya leaf juice (CPLJ) was well known for its thrombocytosis activity in rodents and dengue patients. However, the effect of CPLJ treatment on other parameters that could contribute to dengue pathogenesis such as nonstructural protein 1 (NS1) production and viremia level have never been highlighted in any clinical and in vivo studies. The aim of this study is to investigate the effect of freeze-dried CPLJ treatment on NS1 and viremia levels of dengue fever mouse model. Read More

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December 2018

IN VITRO ANALYSIS OF SYNTHETIC PEPTIDES IN BLOCKING THE ENTRY OF DENGUE VIRUS.

Virus Res 2018 Nov 30. Epub 2018 Nov 30.

Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. Electronic address:

Dengue fever is the most prevalent arthropod-borne viral disease, and no specific therapeutic or promising antiviral drug is available for its treatment. Peptide inhibitors are less toxic than synthetic compounds and have found proven effective against viral infections. Here, three peptides that mimic part of the E protein of the dengue virus (DENV) were synthesized and evaluated for their inhibitory activity against four serotypes of DENV in African green monkey kidney (Vero) and rhesus macaque (Macaca mulatta) monkey kidney (LLC-MK2) cell lines. Read More

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November 2018
2 Reads

The Composition of Midgut Bacteria in Aedes aegypti (Diptera: Culicidae) That Are Naturally Susceptible or Refractory to Dengue Viruses.

J Insect Sci 2018 Nov 1;18(6). Epub 2018 Nov 1.

C2D2 Research Group, Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada.

The composition, abundance, and diversity of midgut bacteria in mosquitoes can influence pathogen transmission. We used 16S rRNA microbiome profiling to survey midgut microbial diversity in pooled samples of laboratory colonized dengue-refractory, Cali-MIB, and dengue-susceptible, Cali-S Aedes aegypti (Linnaeus). The 16S rRNA sequences from the sugar-fed midguts of adult females clustered to 63 amplicon sequence variants (ASVs), primarily from Proteobacteria, Firmicutes, Flavobacteria, and Actinobacteria. Read More

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November 2018
1 Read

Epidemiological characteristics and determinants of dengue transmission during epidemic and non-epidemic years in Fortaleza, Brazil: 2011-2015.

PLoS Negl Trop Dis 2018 Dec 3;12(12):e0006990. Epub 2018 Dec 3.

Department of Global Health and Population, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, United States of America.

Background: After being eliminated during the 1950s, dengue reemerged in Brazil in the 1980s. Since then, incidence of the disease has increased, as serotypes move within and between cities. The co-circulation of multiple serotypes contributes to cycles of epidemic and interepidemic years, and a seasonal pattern of transmission is observed annually. Read More

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December 2018
1 Read

Post Dengue Fever Epidural Abscess With or Without Spondylodiscitis: A Case Series of Eight Patients in a Single Season in a Single Center: Does the Dengue Have a Predilection for the Neural Tissue?

Spine (Phila Pa 1976) 2018 Dec;43(24):E1454-E1462

Aster MIMS Hospital, Calicut, Kerala, India.

Study Design: A retrospective case series.

Objective: The aim of this study was to report the immunosuppression and secondary bacterial infection after dengue viral infection and the predilection of these infections in the spine. This can trigger further research in to this fact. Read More

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December 2018
2 Reads

Beyond thrombocytopaenia, haemorrhage and shock: the expanded dengue syndrome.

Pathog Glob Health 2018 Dec 3:1-11. Epub 2018 Dec 3.

c Department of Parasitology, Faculty of medicine , University of Colombo , Colombo , Sri Lanka.

Dengue infection classically presents with fever, thrombocytopaenia, and varying degrees of plasma leakage, giving rise to shock. However, a myriad of other manifestations, involving the cardiovascular system, the nervous system, the liver, the kidneys, the gut and the haematological system have been reported in dengue. This review summarizes these varied presentations. Read More

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December 2018
4 Reads

Epidemiology and Outcomes of Dengue in Kidney Transplant Recipients: A 20-year Retrospective Analysis and Comparative Literature Review.

Clin Transplant 2018 Dec 2:e13458. Epub 2018 Dec 2.

Division of Infectious Diseases, Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.

Background: Kidney transplant (KT) recipients in dengue-endemic areas are at risk of exposure. We investigated the epidemiology and outcomes from dengue in KT recipients at our transplant center and conducted a literature review.

Materials And Methods: We conducted a 20-year retrospective study of KT recipients who were diagnosed with laboratory-confirmed dengue from January 1997 to September 2017 according to the 2009 WHO classification. Read More

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December 2018
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Detection and Differentiation of Multiple Viral RNAs Using Branched DNA FISH Coupled to Confocal Microscopy and Flow Cytometry.

Bio Protoc 2018 Oct;8(20)

Department of Genetics, Stanford University School of Medicine, Stanford, CA, 94305, USA.

Due to the exceptionally high mutation rates of RNA-dependent RNA polymerases, infectious RNA viruses generate extensive sequence diversity, leading to some of the lowest barriers to the development of antiviral drug resistance in the microbial world. We have previously discovered that higher barriers to the development of drug resistance can be achieved through dominant suppression of drug-resistant viruses by their drug-susceptible parents. We have explored the existence of dominant drug targets in poliovirus, dengue virus and hepatitis C virus (HCV). Read More

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October 2018
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Zika virus infection in human placental tissue explants is enhanced in the presence of dengue virus antibodies in-vitro.

Emerg Microbes Infect 2018 Dec 1;7(1):198. Epub 2018 Dec 1.

Institute of Virology, Campus Charité Mitte, Charité - Universitätsmedizin Berlin, Berlin, Germany.

The current Zika virus (ZIKV) outbreak is associated with neurological malformations and disorders in neonates. Areas of increased incidence of malformations may overlap with dengue-hyperendemic areas. ZIKV infection is enhanced by antibodies against dengue virus (DENV) in cell culture and inbred mice. Read More

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December 2018
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Autopsy in dengue encephalitis: An analysis of three cases.

Neurol India 2018 Nov-Dec;66(6):1721-1725

Department of Pathology and Neurology, Command Hospital Air Force, Bengaluru, Karnataka, India.

Dengue viral infection with unusual presentations has been described in literature, but autopsy is rarely done and is only limited to some cases, based on the literature review. Here, we present the autopsy findings in three cases of dengue encephalitis. All the three patients clinically presented with signs of meningoencephalitis and were positive for dengue non-structural (NS) 1 antigen (Ag) in the serum and cerebrospinal fluid. Read More

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December 2018
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Immune-mediated neurological manifestations of dengue virus- a study of clinico-investigational variability, predictors of neuraxial involvement, and outcome with the role of immunomodulation.

Neurol India 2018 Nov-Dec;66(6):1634-1643

Department of Neurology, Government Medical College Kota, Rajasthan, India.

Introduction: Our aim was to study dengue-related immune-mediated neurological complications (IMNC) during the recent epidemic.

Materials And Methods: This was a cross-sectional observational study of 79 IMNC cases from 1627 laboratory confirmed dengue cases from January 2015 to January 2016 and their follow-up for 3 months. According to the World Health Organization, cases were categorized into those having dengue fever (DF), and those having a severe syndrome that includes dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Read More

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December 2018
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