47,584 results match your criteria Cytomegalovirus Esophagitis


High-Temperature Short-Time Treatment of Human Milk for Bacterial Count Reduction.

Front Pediatr 2018 27;6:359. Epub 2018 Nov 27.

Center for Pediatrics, Department of Neonatology, Faculty of Medicine, Medical Center - University of Freiburg, Freiburg, Germany.

Human milk (HM) for preterm infants will often be pasteurized for cytomegalovirus (CMV) inactivation and reduction of its bacterial count. High-temperature short-time (HTST) treatment compared to standard Holder pasteurization (HoP) reduces the impact of heat treatment on bioactive HM proteins while effectively inactivating CMV. No data are available for the efficacy of bacterial count reduction using HTST treatments that are available for clinical use. Read More

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November 2018

Histone deacetylase inhibitors reactivate silenced transgene in vivo.

Gene Ther 2018 Dec 11. Epub 2018 Dec 11.

Department of Pharmaceutical and Biomedical Sciences, University of Georgia College of Pharmacy, Athens, Georgia, 30602, USA.

Gene silencing for plasmid-based vectors and the underlying mechanism are critical factors for development of effective gene therapy. The objective of this study is to explore the role of epigenetic regulation for transgene expression. Two reporter genes, mouse interleukin 10 and human secreted alkaline phosphatase under the control of human cytomegalovirus immediate early promoter for expression, were delivered to mouse liver by hrodydynamics-based procedure and reporter gene expression was monitored. Read More

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December 2018

Is antibody-mediated rejection in kidney transplant recipients a risk factor for developing cytomegalovirus or BK virus infection? Results from a case-control study.

J Clin Virol 2018 Dec 1;110:45-50. Epub 2018 Dec 1.

Infectious Diseases Department, Vall d'Hebron University Hospital, Barcelona, Spain; Departament of Medicine, Universitat Autònoma de Barcelona, Barcelona, Spain.

Background: Data are scarce on cytomegalovirus (CMV) and BK virus (BKV) infection after antibody-mediated rejection (ABMR).

Objectives: We hypothesized that the immunological response in patients with ABMR or the immune modulation associated with its treatment could predispose to CMV and BKV infection. Our objective was to investigate this hypothesis. Read More

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December 2018
1 Read

Human rhinovirus infections in hematopoietic cell transplant recipients: risk score for progression to lower respiratory tract infection.

Biol Blood Marrow Transplant 2018 Dec 8. Epub 2018 Dec 8.

Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N Seattle, WA 98109. Electronic address:

Human rhinovirus lower respiratory tract infection is associated with mortality following hematopoietic cell transplantation; however, risk factors for lower respiratory tract infection are not well characterized. We sought to develop a risk score for progression to lower respiratory tract infection from upper respiratory tract infection in hematopoietic cell transplantation recipients. Risk factors for lower respiratory tract infection within 90 days were analyzed using Cox regression among hematopoietic cell transplantation recipients with rhinovirus upper respiratory tract infection between 1/2009-3/2016. Read More

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December 2018

Blood Viral Load in Symptomatic Congenital Cytomegalovirus Infection.

J Infect Dis 2018 Dec 7. Epub 2018 Dec 7.

Department of Pediatrics, Division of Infectious Diseases, University of Alabama at Birmingham, Birmingham, AL, USA.

Background: Viral loads (VLs) frequently are followed during treatment of symptomatic congenital cytomegalovirus disease, but their predictive value is unclear.

Methods: Post-hoc analysis of two antiviral studies was performed. Seventy-three subjects were treated for 6 weeks and 47 subjects for 6 months. Read More

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December 2018
1 Read

Cytomegalovirus reactivation triggers the late onset of hyperthyroidism after autologous peripheral blood transplantation.

Leuk Res Rep 2019 22;11:5-7. Epub 2018 Nov 22.

Division of Hematology, Japanese Red Cross Society Wakayama Medical Center, Wakayama, Wakayama, Japan.

Thyroid dysfunction is an important issue in patients receiving autologous and allogenic hematopoietic stem cell transplantation (HSCT). However, the exact mechanisms underlying thyrotoxicosis secondary to HSCT remain unclear. The present case exhibited a reversed imbalance in helper/suppressor T-cell populations and B-cell dysregulation for a long time after transplantation, and the reactivation of cytomegalovirus may have been associated with the development of clinical hyperthyroidism. Read More

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November 2018

Activation of E2F-dependent transcription by the mouse cytomegalovirus M117 protein affects the viral host range.

PLoS Pathog 2018 Dec 10;14(12):e1007481. Epub 2018 Dec 10.

Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.

Cytomegaloviruses (CMVs) have a highly restricted host range as they replicate only in cells of their own or closely related species. To date, the molecular mechanisms underlying the CMV host restriction remain poorly understood. However, it has been shown that mouse cytomegalovirus (MCMV) can be adapted to human cells and that adaptation goes along with adaptive mutations in several viral genes. Read More

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December 2018

STAT6 degradation and ubiquitylated TRIML2 are essential for activation of human oncogenic herpesvirus.

PLoS Pathog 2018 Dec 10;14(12):e1007416. Epub 2018 Dec 10.

MOE& MOH Key Laboratory of Medical Molecular Virology, School of Basic Medicine, Shanghai Medical College, Fudan University, Shanghai, P. R. China.

Aberrations in STAT6-mediated signaling are linked to the development of multiple cancer types. Increasing evidence has shown that activation of human oncogenic herpesvirus lytic replication is crucial for viral tumorigenesis. However, the role of STAT6 in herpesvirus lytic replication remains elusive. Read More

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December 2018

Medical Referral Patterns and Etiologies for Children With Mild-to-Severe Hearing Loss.

Ear Hear 2018 Dec 6. Epub 2018 Dec 6.

Department of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine in St. Louis, St. Louis, Missouri, USA.

Objectives: To (1) identify the etiologies and risk factors of the patient cohort and determine the degree to which they reflected the incidence for children with hearing loss and (2) quantify practice management patterns in three catchment areas of the United States with available centers of excellence in pediatric hearing loss.

Design: Medical information for 307 children with bilateral, mild-to-severe hearing loss was examined retrospectively. Children were participants in the Outcomes of Children with Hearing Loss (OCHL) study, a 5-year longitudinal study that recruited subjects at three different sites. Read More

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December 2018

Human cytomegalovirus US21 protein is a viroporin that modulates calcium homeostasis and protects cells against apoptosis.

Proc Natl Acad Sci U S A 2018 Dec 10. Epub 2018 Dec 10.

Department of Life Sciences and Systems Biology, University of Torino, 10123 Torino, Italy

The human cytomegalovirus (HCMV) US12 gene family comprises a set of 10 contiguous genes (US12 to US21) with emerging roles in the regulation of virus cell tropism, virion composition, and immunoevasion. Of all of the US12 gene products, pUS21 shows the highest level of identity with two cellular transmembrane BAX inhibitor motif-containing (TMBIM) proteins: Bax inhibitor-1 and Golgi anti-apoptotic protein, both of which are involved in the regulation of cellular Ca homeostasis and adaptive cell responses to stress conditions. Here, we report the US21 protein to be a viral-encoded ion channel that regulates intracellular Ca homeostasis and protects cells against apoptosis. Read More

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December 2018

The antibiotic resistance-free mammalian expression plasmid vector pPAL for development of third generation vaccines.

Plasmid 2018 Dec 6. Epub 2018 Dec 6.

Laboratory of Molecular Parasitology, Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (Consejo Superior de Investigaciones Científicas). Calle Ramiro de Maeztu, 9, 28040 Madrid, Spain. Electronic address:

DNA vaccines require a vector to replicate genes and express encoding antigens. Antibiotic resistance genes are often used as selection markers, which must not be released to the environment upon final product commercialization. For this reason, generation of antibiotic resistance-free vectors is imperative. Read More

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December 2018

Initial intravitreal injection of high-dose ganciclovir for cytomegalovirus retinitis in HIV-negative patients.

BMC Ophthalmol 2018 Dec 10;18(1):314. Epub 2018 Dec 10.

Shanghai Aier Eye Hospital, Shanghai, China, No. 1286, Hongqiao Road, Changning District, Shanghai, 200050, China.

Background: The purpose of this study is to examine the clinical outcomes achieved by using initial high-dose intravitreal ganciclovir injections to treat cytomegalovirus retinitis in patients without human immunodeficiency virus (HIV) infection.

Methods: Twenty-four eyes (24 patients) with cytomegalovirus retinitis received multiple intravitreal injections of ganciclovir in weekly intervals. A higher dose (6 mg) of ganciclovir was applied at the first intravitreal injection, and a lower dose was used for maintenance. Read More

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December 2018

Allogeneic stem cell transplantation with reduced intensity conditioning for patients with adrenoleukodystrophy.

Mol Genet Metab Rep 2019 Mar 20;18:1-6. Epub 2018 Nov 20.

Department of Hematology and Oncology, Children' Medical Center, Japanese Red Cross Nagoya First Hospital, Nagoya, Japan.

Objective: The prognosis of adrenoleukodystrophy (ALD)with neurological involvement is generally dismal; however, allogeneic stem cell transplantation (SCT) is recognized as effective to stabilize or improve the clinical symptoms of ALD. Herein, we report the clinical outcomes of patients with ALD who consecutively underwent allogeneic stem cell transplantation with reduced intensity conditioning at our institution.

Patients: Sixteen patients with ALD, who were symptomatic (n = 14) or presymptomatic (n = 2), received SCT from 2010 to 2016. Read More

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March 2019
3 Reads

Intrahost dynamics of human cytomegalovirus variants acquired by seronegative glycoprotein B vaccinees.

J Virol 2018 Dec 5. Epub 2018 Dec 5.

Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, USA.

Human cytomegalovirus (HCMV) is the most common congenital infection worldwide, and a frequent cause of hearing loss and debilitating neurologic disease in newborn infants. Thus, a vaccine to prevent HCMV-associated congenital disease is a public health priority. One potential strategy is vaccination of women of child-bearing age to prevent maternal HCMV acquisition during pregnancy. Read More

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December 2018

Different functional states of fusion protein gB revealed on human cytomegalovirus by cryo electron tomography with Volta phase plate.

PLoS Pathog 2018 Dec 3;14(12):e1007452. Epub 2018 Dec 3.

Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, California, United States of America.

Human cytomegalovirus (HCMV) enters host by glycoprotein B (gB)-mediated membrane fusion upon receptor-binding to gH/gL-related complexes, causing devastating diseases such as birth defects. Although an X-ray crystal structure of the recombinant gB ectodomain at postfusion conformation is available, the structures of prefusion gB and its complex with gH/gL on the viral envelope remain elusive. Here, we demonstrate the utility of cryo electron tomography (cryoET) with energy filtering and the cutting-edge technologies of Volta phase plate (VPP) and direct electron-counting detection to capture metastable prefusion viral fusion proteins and report the structures of glycoproteins in the native environment of HCMV virions. Read More

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December 2018
4 Reads

Development of a predictive model using endoscopic features for gastric cytomegalovirus infection in renal transplant patients.

Transplantation 2018 Nov 30. Epub 2018 Nov 30.

Division of Nephrology, Department of Internal Medicine, Keimyung University School of Medicine, Daegu, Korea.

Background: Cytomegalovirus (CMV) is a common viral pathogen in transplant patients that often targets the stomach. However, the endoscopic characteristics of gastric CMV infection are not well established. We aimed to develop a predictive model using endoscopic findings for gastric CMV infection in renal transplant patients. Read More

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November 2018
1 Read

HIV, HCMV and mycobacterial antibody levels: a cross-sectional study in a rural Ugandan cohort.

Trop Med Int Health 2018 Dec 1. Epub 2018 Dec 1.

Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, UK.

Objectives: A growing evidence base implicates human cytomegalovirus (HCMV) as a risk factor for TB disease. We investigated total IgG and mycobacteria-specific antibodies in a cross-sectional study nested within a rural Ugandan General Population Cohort (GPC), in relation to HIV infection and the magnitude of HCMV IgG response.

Methods: Sera from 2,189 individuals (including 27 sputum-positive TB cases) were analysed for antibodies against mycobacteria (Ag85A, PPD, LAM, ESAT6/CFP10) and HCMV, tetanus toxoid (TT) and total IgG. Read More

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December 2018
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The detection of the cytomegalovirus DNA in the colonic mucosa of patients with ulcerative colitis is associated with increased long-term risk of proctocolectomy: results from an outpatient IBD clinic.

Int J Colorectal Dis 2018 Nov 30. Epub 2018 Nov 30.

Internistische Gemeinschaftspraxis für Verdauungs- und Stoffwechselkrankheiten, Leipzig und Schkeuditz, Leipzig, Germany.

Purpose: Cytomegalovirus (CMV) infection has been found to be associated with a reactivation of ulcerative colitis (UC) and with an impaired response to medical therapy. In the past, only limited data were available on the long-term outcome for UC patients with positive tissue CMV-PCR in the colonic mucosa.

Methods: Between January 2010 and April 2015, we performed a qualitative PCR screening for CMV DNA in one biopsy from most actively inflamed rectal mucosa (tCMV-PCR). Read More

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November 2018
1 Read
2.449 Impact Factor

Synthesis of new betulinic acid/betulin-derived dimers and hybrids with potent antimalarial and antiviral activities.

Bioorg Med Chem 2018 Nov 14. Epub 2018 Nov 14.

Organic Chemistry Chair I and Interdisciplinary Center for Molecular Materials (ICMM), Friedrich-Alexander University of Erlangen-Nürnberg, Nikolaus-Fiebiger-Straße 10, 91058 Erlangen, Germany. Electronic address:

Severe malaria and viral infections cause life-threatening diseases in millions of people worldwide every year. In search for effective bioactive hybrid molecules, which may possess improved properties compared to their parent compounds, a series of betulinic acid/betulin based dimer and hybrid compounds carrying ferrocene and/or artesunic acid moieties, was designed and, synthesized de novo. Furthermore, they were analyzed in vitro against malaria parasites (growth inhibition of 3D7-strain P. Read More

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November 2018

Clinical and economic impact of the cytomegalovirus infection among children undergoing allogeneic hematopoietic cell transplantation.

Biol Blood Marrow Transplant 2018 Nov 28. Epub 2018 Nov 28.

Division of Pediatric Hematology, Oncology, and Stem Cell Transplantation, Department of Pediatrics, Columbia University Medical Center, New York, NY. Electronic address:

Literature on the impact of cytomegalovirus (CMV)-related hospitalization in allogeneic hematopoietic cell transplant (alloHCT) pediatric patients is limited. The aim of this study was to determine utilization and outcomes of CMV-related hospitalization in alloHCT patients using a single-center clinical database. This was a retrospective study of 240 children ages 3 months to 21 years old who were transplanted between 2005 and 2016. Read More

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November 2018

Splenic infarction due to cytomegalovirus infection in an immunocompetent patient.

Med Clin (Barc) 2018 Nov 27. Epub 2018 Nov 27.

Servicio de Medicina Interna, Hospital Garcia Orcoyen, Estella, Navarra, España.

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November 2018

Human cytomegalovirus utilises cellular dual-specificity tyrosine phosphorylation-regulated kinases during placental replication.

Placenta 2018 Dec 13;72-73:10-19. Epub 2018 Oct 13.

Serology and Virology Division, SEALS Microbiology, Prince of Wales Hospital, Sydney, Australia; School of Women's and Children's Health, University of New South Wales, Australia; School of Medical Sciences, University of New South Wales, Australia; School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia. Electronic address:

Introduction: Congenital cytomegalovirus (HCMV) infection may cause significant fetal malformation and in severe cases fetal and neonatal death. Fetal injury may be caused indirectly by the placental response to infection. Dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs) have recently been identified as critical kinases for HCMV replication. Read More

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December 2018
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[Efficacy Analysis of Unrelated Umbilical Cord Blood Transplantation for the Treatment of Wiskott-Aldrich Syndrome].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2018 Dec;26(6):1816-1821

Department of Hematology,Zhongda Hospital,Southeast University,Nanjing 210009,Jiangsu Province, China.

Objective: To explore the clinical efficacy and safety of unrelated umbilical cord blood transplantation (UCBT) for the treatment of Wiskott-Aldrich syndrome(WAS).

Methods: Five pediatric patients with WAS received single UCBT were retrospectively analyzed. The median age of these male patients was 268 days (range, 3 days -695 days). Read More

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December 2018
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CMV Primes Functional Alternative Signaling in Adaptive Δg NK Cells but Is Subverted by Lentivirus Infection in Rhesus Macaques.

Cell Rep 2018 Dec;25(10):2766-2774.e3

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA; Ragon Institute of Massachusetts General Hospital, MIT, and Harvard, Cambridge, MA 02139, USA. Electronic address:

Despite burgeoning evidence demonstrating the adaptive properties of natural killer (NK) cells, mechanistic data explaining these phenomena are lacking. Following antibody sensitization, NK cells lacking the Fc receptor (FcR) signaling chain (Δg) acquire adaptive features, including robust proliferation, multifunctionality, rapid killing, and mobilization to sites of virus exposure. Using the rhesus macaque model, we demonstrate the systemic distribution of Δg NK cells expressing memory features, including downregulated Helios and Eomes. Read More

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December 2018
1 Read

Absence of cytomegalovirus in gingivitis and chronic periodontitis in HIV-1 patients in Northern Brazil.

Rev Soc Bras Med Trop 2018 Nov-Dec;51(6):809-812

Departamento de Periodontologia, Centro Universitário do Estado do Pará, Belém, PA, Brasil.

Introduction: The influence of cytomegalovirus (CMV) on the progression of chronic periodontitis in HIV patients is poorly investigated.

Methods: ELISA was used for anti-CMV antibody IgG titer measurements and real-time polymerase chain reaction for qualitative and quantitative CMV detection. Data on the CD4 + T lymphocyte count and plasma HIV viral load were obtained from patient records. Read More

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February 2018

Immunosenescence of Natural Killer Cells, Inflammation, and Alzheimer's Disease.

Int J Alzheimers Dis 2018 1;2018:3128758. Epub 2018 Nov 1.

Instituto Maimónides de Investigación Biomédica (IMIBIC), Córdoba, Spain.

Alzheimer's disease (AD) represents the most common cause of dementia in the elderly. AD is a neurodegenerative disorder characterized by progressive memory loss and cognitive decline. Although the aetiology of AD is not clear, both environmental factors and heritable predisposition may contribute to disease occurrence. Read More

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November 2018
6 Reads

HIV-1 DNA is Maintained in Antigen-Specific CD4+ T Cell Subsets in Patients on Long-Term Antiretroviral Therapy Regardless of Recurrent Antigen Exposure.

AIDS Res Hum Retroviruses 2018 Dec 4. Epub 2018 Dec 4.

St. Vincent's Centre for Applied Medical Research, HIV Immunovirology, Sydney, New South Wales, Australia.

Background: Memory CD4+ T cells (mCD4s) containing integrated HIV DNA are considered the main barrier to a cure for HIV infection. Here we analysed HIV DNA reservoirs in antigen-specific subsets of mCD4s to delineate the mechanisms by which HIV reservoirs persist during ART.

Methods/results: HIV Gag, Cytomegalovirus (CMV) and Tetanus Toxoid (TT) specific mCD4s were isolated from peripheral blood samples obtained from 11 individual subjects, 2-11 years after commencing ART. Read More

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December 2018
2 Reads

Cytomegalovirus pseudotumor of the colon in an HIV patient.

Radiol Case Rep 2019 Feb 27;14(2):273-277. Epub 2018 Nov 27.

Department of Radiology, Hospital Garcia de Orta, EPE, Av. Torrado da Silva, Almada 2805-267, Portugal.

Cytomegalovirus (CMV) is the most common cause of severe opportunistic viral disease among patients with acquired immunodeficiency syndrome, and colitis is the most frequent manifestation of CMV infection. Nevertheless, the development of a colonic pseudotumor is a rare benign entity that can be easily misdiagnosed as a colonic neoplasm if the radiologist is not aware of this condition. We present a case of a 42-year-old male with a CMV pseudotumor of the colon. Read More

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February 2019
3 Reads

Downregulation of the central noradrenergic system by infection.

Infect Immun 2018 Dec 3. Epub 2018 Dec 3.

School of Biology, Faculty of Biological Sciences, Clarendon Way, University of Leeds, Leeds LS2 9JT, UK

is associated with physiological effects in the host. Dysregulation of catecholamines in the central nervous system has previously been observed in chronically-infected animals. In the study described here, the noradrenergic system was found to be suppressed with decreased levels of norepinephrine (NE) in brains of infected animals and in infected human and rat neural cells The mechanism responsible for the NE suppression was found to be down-regulation of dopamine β-hydroxylase (DBH) gene expression, encoding the enzyme that synthesizes norepinephrine from dopamine with down-regulation observed and in infected brain tissue, particularly in the dorsal locus coeruleus/pons region. Read More

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December 2018
3 Reads

Detection of glycoproteins B and H genotypes to predict the development of Cytomegalovirus disease in solid organ transplant recipients.

J Clin Virol 2018 Dec 13;109:50-56. Epub 2018 Nov 13.

Virology Laboratory, Clinical Microbiology Department, University Hospital 12 de Octubre, Avda. de Córdoba s/n, 28041 Madrid, Spain; Biomedical Research Institute i+12, University Hospital 12 de Octubre, Avda. de Córdoba, s/n, 28041 Madrid, Spain; Department of Medicine, School of Medicine, Complutense University, Pl. Ramón y Cajal, s/n, 28040 Madrid, Spain.

Background: Our study focuses on the role that human Cytomegalovirus (CMV) genotypes play in the development of disease.

Objectives: (1) To analyze the frequency of various genotype envelope proteins (gB, gH) in a group of solid organ transplant (SOT) recipients; (2) to assess their correlation with CMV disease; (3) to study the association between any of the genotypes and viral loads.

Study Design: A retrospective observational study conducted by analyzing CMV gB and gH genotypes detected with real-time polymerase chain reaction (PCR)-specific assays in 162 CMV-positive blood samples from 62 SOT recipients. Read More

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December 2018

Placental Cytomegalovirus Infection.

Arch Pathol Lab Med 2018 Nov 30. Epub 2018 Nov 30.

From the Department of Pathology, University of Colorado, Anschutz Medical Campus, Aurora (Dr Lindholm); and the Department of Pathology, Denver Health Medical Center, Denver, Colorado (Dr O'Keefe).

In the United States, cytomegalovirus is the most common congenital viral infection and the number 1 cause of nonhereditary sensorineural hearing loss. The vast majority of infants may be asymptomatic, especially if cytomegalovirus is contracted later in the pregnancy, and some symptoms may have a delayed onset. Therefore, it is important for the pathologist to identify the common histologic findings to help confirm the diagnosis so the child can be followed for late sequelae. Read More

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November 2018
2 Reads

Viral M45 and necroptosis-associated proteins form heteromeric amyloid assemblies.

EMBO Rep 2018 Nov 29. Epub 2018 Nov 29.

Discipline of Pharmacology, School of Medical Sciences, Faculty of Medicine and Health and Sydney Nano, University of Sydney, Sydney, NSW, Australia

The murine cytomegalovirus protein M45 protects infected mouse cells from necroptotic death and, when heterologously expressed, can protect human cells from necroptosis induced by tumour necrosis factor receptor (TNFR) activation. Here, we show that the N-terminal 90 residues of the M45 protein, which contain a RIP homotypic interaction motif (RHIM), are sufficient to confer protection against TNFR-induced necroptosis. This N-terminal region of M45 drives rapid self-assembly into homo-oligomeric amyloid fibrils and interacts with the RHIMs of the human kinases RIPK1 and RIPK3, and the Z-DNA binding protein 1 (ZBP1), to form heteromeric amyloid fibrils Mutation of the tetrad residues in the M45 RHIM attenuates homo- and hetero-amyloid assembly by M45, suggesting that the amyloidogenic nature of the M45 RHIM underlies its biological activity. Read More

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November 2018
1 Read

Xanthine-based acyclic nucleoside phosphonates with potent antiviral activity against varicella-zoster virus and human cytomegalovirus.

Antivir Chem Chemother 2018 Jan-Dec;26:2040206618813050

1 Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic.

While noncanonic xanthine nucleotides XMP/dXMP play an important role in balancing and maintaining intracellular purine nucleotide pool as well as in potential mutagenesis, surprisingly, acyclic nucleoside phosphonates bearing a xanthine nucleobase have not been studied so far for their antiviral properties. Herein, we report the synthesis of a series of xanthine-based acyclic nucleoside phosphonates and evaluation of their activity against a wide range of DNA and RNA viruses. Two acyclic nucleoside phosphonates within the series, namely 9-[2-(phosphonomethoxy)ethyl]xanthine (PMEX) and 9-[3-hydroxy-2-(phosphonomethoxy)propyl]xanthine (HPMPX), were shown to possess activity against several human herpesviruses. Read More

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December 2018

IMMUNE RECOVERY UVEITIS-LIKE SYNDROME MIMICKING RECURRENT T-CELL LYMPHOMA AFTER AUTOLOGOUS BONE MARROW TRANSPLANT.

Retin Cases Brief Rep 2018 Nov 26. Epub 2018 Nov 26.

Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, Ohio.

Purpose: To report the multimodal imaging findings of immune recovery uveitis mimicking recurrent T-cell lymphoma after autologous bone marrow transplant therapy.

Methods: A 71-year-old man presented with posterior uveitis 6 weeks after chemotherapy and autologous bone marrow transplant for angioimmunoblastic T-cell lymphoma. Multimodal imaging included fluorescein angiography, fundus autofluorescence, and optical coherence tomography. Read More

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November 2018
2 Reads

Detection of herpesvirus capsids in transmission electron microscopy images using transfer learning.

Histochem Cell Biol 2018 Nov 28. Epub 2018 Nov 28.

Central Facility for Electron Microscopy, Ulm University, Ulm, Germany.

The detailed analysis of secondary envelopment of the Human betaherpesvirus 5/human cytomegalovirus (HCMV) from transmission electron microscopy (TEM) images is an important step towards understanding the mechanisms underlying the formation of infectious virions. As a step towards a software-based quantification of different stages of HCMV virion morphogenesis in TEM, we developed a transfer learning approach based on convolutional neural networks (CNNs) that automatically detects HCMV nucleocapsids in TEM images. In contrast to existing image analysis techniques that require time-consuming manual definition of structural features, our method automatically learns discriminative features from raw images without the need for extensive pre-processing. Read More

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November 2018
2 Reads

Prevalence of Transfusion-transmitted Virus (TTV) Infection and its Association with Renal Post-transplantation Complications in Iran.

Int J Organ Transplant Med 2018 1;9(3):126-131. Epub 2018 Aug 1.

Gametogenesis Research center, Kashan University of Medical Sciences, Kashan, Iran.

Background: Transfusion-transmitted virus (TTV) is a single-stranded DNA virus. Renal transplant patients have a higher risk of TTV infection.

Objective: To evaluate the prevalence of TTV and its correlation with post-renal transplantation complications in a population of Iranian patients. Read More

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August 2018
6 Reads

Interaction between Herpes Virus Infections and IL10 and Risk of Bone Marrow Suppression.

Int J Organ Transplant Med 2018 1;9(3):119-125. Epub 2018 Aug 1.

Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran.

Background: Syndrome of transient bone marrow suppression may result from various extra-hematological diseases, such as immunological deregulations, and viral infectious diseases secondarily affecting the function of hematopoietic stem cells.

Objective: To evaluate the pathogenic role of herpes viruses and their contraction with cytokine gene polymorphism, which can impair hematopoiesis in patients with transient bone marrow suppression.

Methods: In a cross-sectional study 30 patients who admitted to Namazi Hospital, affiliated to Shiraz University of Medical Sciences, with transient bone marrow suppression were recruited. Read More

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August 2018
2 Reads

A Survey of Infection in Allogenic Hematopoietic Stem Cell Transplantation in Patients with Acute Myeloid Leukemia.

Int J Organ Transplant Med 2018 1;9(3):112-116. Epub 2018 Aug 1.

Researcher at Tehran University of Medical Sciences, Tehran, Iran.

Background: Allogeneic hematopoietic stem cell transplantation (HSCT) is a potentially cure for acute myeloid leukemia (AML). Patients who undergone HSCT are at increased risk of infection due to impaired immunity.

Objective: To evaluate the rate of bacterial, viral and fungal infection and its relationship with 2-year overall survival of AML patients who had undergone HSCT. Read More

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August 2018
4 Reads

Major TCR Repertoire Perturbation by Immunodominant HLA-B44:03-Restricted CMV-Specific T Cells.

Front Immunol 2018 14;9:2539. Epub 2018 Nov 14.

Department of Paediatrics, University of Oxford, Oxford, United Kingdom.

Lack of disease during chronic human cytomegalovirus (CMV) infection depends on the maintenance of a high-frequency CMV-specific T cell response. The composition of the T cell receptor (TCR) repertoire underlying this response remains poorly characterised, especially within African populations in which CMV is endemic from infancy. Here we focus on the immunodominant CD8+ T cell response to the immediate-early 2 (IE-2)-derived epitope NEGVKAAW (NW8) restricted by HLA-B44:03, a highly prevalent response in African populations, which in some subjects represents >10% of the circulating CD8+ T cells. Read More

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November 2018
1 Read

Cytomegalovirus infection in ulcerative colitis assessed by quantitative polymerase chain reaction: risk factors and effects of immunosuppressants.

J Clin Biochem Nutr 2018 Nov 8;63(3):246-251. Epub 2018 Jun 8.

2nd Department of Internal Medicine, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan.

We investigated the risk factors of and appropriate treatment for cytomegalovirus colitis in patients with ulcerative colitis, using quantitative polymerase chain reaction analysis to detect cytomegalovirus in the colonic mucosa. Between February 2013 and January 2017, patients with exacerbated ulcerative colitis who were admitted to our hospital were consecutively enrolled in this retrospective, single-center study. Patients were evaluated for cytomegalovirus using serology (antigenemia) and quantitative polymerase chain reaction analyses of the colonic mucosa, which were sampled during colonoscopy. Read More

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November 2018
2 Reads

A gB/CD3 bispecific BiTE antibody construct for targeting Human Cytomegalovirus-infected cells.

Sci Rep 2018 Nov 28;8(1):17453. Epub 2018 Nov 28.

Children's Cancer Research Institute, Vienna, Austria.

Bispecific T cell engager (BiTE) antibody constructs are successfully used as cancer therapeutics. We hypothesized that this treatment strategy could also be applicable for therapy of human cytomegalovirus (HCMV) infection, since HCMV-encoded proteins are abundantly expressed on the surface of infected cells. Here we show that a BiTE antibody construct directed against HCMV glycoprotein B (gB) and CD3 efficiently triggers T cells to secrete IFN-γ and TNF upon co-culture with fibroblasts infected with HCMV strain AD169, Towne or Toledo. Read More

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November 2018
8 Reads

Restriction of human cytomegalovirus infection by galectin-9.

J Virol 2018 Nov 28. Epub 2018 Nov 28.

Discipline of Infectious Diseases and Immunology, University of Sydney, Camperdown, New South Wales, Australia.

Human cytomegalovirus (HCMV) is a ubiquitous human herpesvirus. While HCMV infection is generally asymptomatic in the immunocompetent, it can have devastating consequences in those with compromised or under-developed immune systems, including transplant recipients and neonates. Galectins are a widely expressed protein family that have been demonstrated to modulate both anti-viral immunity and regulate direct host-virus interactions. Read More

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November 2018

Identification and functional characterization of a novel Fc gamma-binding glycoprotein in Rhesus Cytomegalovirus.

J Virol 2018 Nov 28. Epub 2018 Nov 28.

Vaccine and Gene Therapy Institute, Oregon Health and Science University, 505, NW 185th Ave, Beaverton, OR, 97006

Receptors recognizing the Fc part of immunoglobulin G (FcγRs) are key determinants in antibody-mediated immune responses. Members of the interfere with this immune regulatory network by expressing viral FcγRs (vFcγRs). Human cytomegalovirus (HCMV) encodes four distinct vFcγRs that differ with respect to their IgG-subtype specificity and their impact on antibody-mediated immune function The impact of vFcγRs on HCMV pathogenesis and immunomodulation is not known. Read More

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November 2018
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[The 466th case:myasthenia,fever,blurred vision and pancytopenia].

Zhonghua Nei Ke Za Zhi 2018 Dec;57(12):942-944

Department of Rheumatology and Immunology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.

A 45-year-old woman was admitted to the Department of Rheumatology and Immunology, Peking Union Medical College Hospital, due to weakness of the upper limbs, fever, and blurred vision. She was clinically diagnosed as systemic lupus erythematosus overlapped primary biliary cirrhosis, with renal, retinal, hematological and musculoskeletal involvement, combined with severe pulmonary infection and respiratory failure. Treated with glucocorticoids, ursodeoxycholic acid, antibiotics and respiratory support, the patient got better. Read More

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December 2018
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Efficient and Stable Delivery of Multiple Genes to Fish Cells by a Modified Recombinant Baculovirus System.

Int J Mol Sci 2018 Nov 27;19(12). Epub 2018 Nov 27.

Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.

The recombinant baculovirus has been widely used as an efficient tool to mediate gene delivery into mammalian cells but has barely been used in fish cells. In the present study, we constructed a recombinant baculovirus containing the dual-promoter cytomegalovirus (CMV) and white spot syndrome virus (WSSV) immediate-early gene 1 (ie1) (WSSV ie1), followed by a puromycin⁻green fluorescent protein (Puro-GFP, ) or puromycin⁻red fluorescent protein (Puro-RFP, ) cassette, which simultaneously allowed for easy observation, rapid titer determination, drug selection, and exogenous gene expression. This recombinant baculovirus was successfully transduced into fish cells, including bladder (MPB), fin (MPF), and kidney (MPK); spermatogonia (SG3); and embryonic fibroblast (ZF4) cells. Read More

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November 2018
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Potential Biomarkers for Predicting Congenital Cytomegalovirus Infection.

Int J Mol Sci 2018 Nov 27;19(12). Epub 2018 Nov 27.

Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.

Early diagnosis and treatment of infants with symptomatic congenital cytomegalovirus (CMV) infection may improve neurological outcomes. For this reason, prenatal detection of newborns at high risk for congenital CMV infection is important. A polymerase chain reaction (PCR) assay for CMV DNA in the amniotic fluid is the gold standard for the diagnosis of intrauterine CMV infection; however, amniocentesis is an invasive procedure. Read More

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November 2018
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Natural Killer Cells Adapt to Cytomegalovirus Along a Functionally Static Phenotypic Spectrum in Human Immunodeficiency Virus Infection.

Front Immunol 2018 12;9:2494. Epub 2018 Nov 12.

Immunology and Infectious Diseases Program, Division of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, NL, Canada.

Events related to HCMV infection drive accumulation of functionally enhanced CD57NKG2C adapted NK cells. We investigated NK cell adaptation to HCMV along a proposed continuum progressing from acute activation through maturation and memory formation towards functional exhaustion. Acute exposure to conditioned medium collected 24 h after HCMV infection (HCMVsn) increased NK cell cytotoxicity for all HCMV-seronegative and seropositive donors tested, with mean 38 and 29% boosts in natural and antibody-dependent cell-mediated cytotoxicity (ADCC), respectively. Read More

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November 2018

Severe de novo Ulcerative Colitis following Ixekizumab Therapy.

Case Rep Gastroenterol 2018 Sep-Dec;12(3):617-621. Epub 2018 Oct 17.

Department of Gastroenterology, Staten Island University Hospital, Northwell Health, New York, New York, USA.

Ixekizumab is a selective monoclonal antibody targeting interleukin-17A, approved for the treatment of chronic plaque psoriasis. It has rarely been associated with inflammatory bowel disease (IBD) in randomized trials only. We report a unique case of severe new-onset ulcerative colitis in a young male complicated by cytomegalovirus infection who was on ixekizumab therapy for plaque psoriasis. Read More

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October 2018
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Secondary syphilis presenting as erythema multiforme in a HIV-positive homosexual man: a case report and literature review.

Int J STD AIDS 2018 Nov 27:956462418805197. Epub 2018 Nov 27.

1 Dermatology Hospital of Southern Medical University (Guangdong Provincial Dermatology Hospital), Guangzhou, China.

Early syphilis can rarely cause erythema multiforme-type eruptions as well as triggering erythema multiforme (EM). EM-like lesions in secondary syphilis are characterized by clinical features of EM and laboratory tests consistent with secondary syphilis and the skin histology shows predominantly a plasma cell infiltrate with the presence of treponemes. When EM is triggered by early syphilis, the skin histology shows mixed inflammatory cells usually in the absence of treponemes in the skin lesion. Read More

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November 2018
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