47,870 results match your criteria Cytomegalovirus


hCMV-Mediated Immune Escape Mechanisms Favor Pathogen Growth and Disturb the Immune Privilege of the Eye.

Int J Mol Sci 2019 Feb 16;20(4). Epub 2019 Feb 16.

Institute of Medical Microbiology and Hospital Hygiene, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany.

Human retinal pigment epithelial (hRPE) cells are important for the establishment and maintenance of the immune privilege of the eye. They function as target cells for human cytomegalovirus (hCMV), but are able to restrict viral replication. hCMV causes opportunistic posterior uveitis such as retinitis and chorioretinitis. Read More

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http://dx.doi.org/10.3390/ijms20040858DOI Listing
February 2019

Atomic structures and deletion mutant reveal different capsid-binding patterns and functional significance of tegument protein pp150 in murine and human cytomegaloviruses with implications for therapeutic development.

PLoS Pathog 2019 Feb 19;15(2):e1007615. Epub 2019 Feb 19.

Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, California, United States of America.

Cytomegalovirus (CMV) infection causes birth defects and life-threatening complications in immunosuppressed patients. Lack of vaccine and need for more effective drugs have driven widespread ongoing therapeutic development efforts against human CMV (HCMV), mostly using murine CMV (MCMV) as the model system for preclinical animal tests. The recent publication (Yu et al. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007615DOI Listing
February 2019

Haploidentical Stem Cell Transplantation with Post-Transplant Cyclophosphamide for Primary Immune Deficiency Disorders in Children: Challenges and Outcome from a Tertiary Care Center in South India.

J Clin Immunol 2019 Feb 18. Epub 2019 Feb 18.

Department of Pediatric Hematology, Oncology, Blood and Marrow Transplantation, Apollo Hospitals, 320, Padma complex, Anna salai, Teynampet, Chennai, Tamil Nadu, 600035, India.

Haploidentical stem cell transplantation (haplo SCT) has emerged as an acceptable alternative to matched family donor transplantation for children diagnosed to have primary immune deficiency disorders (PIDs). We present data over 4 years on the challenges and efficacy of unmanipulated T cell replete haplo SCTs with post-transplant cyclophosphamide (PTCy) in children diagnosed to have PIDs. We performed a retrospective study in the pediatric blood and marrow transplantation unit where all children less than 18 years of age diagnosed to have PIDs and who underwent haplo SCT with PTCy from January 2014 to February 2018 were included in the study. Read More

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http://dx.doi.org/10.1007/s10875-019-00600-zDOI Listing
February 2019

Successful Management of Late-Onset Cytomegalovirus-Induced Hemophagocytic Lymphohistiocytosis in Kidney Transplant Recipient After Coronary Artery Bypass Graft Surgery.

Exp Clin Transplant 2019 Jan;17(Suppl 1):207-211

From the Nephrology Department, Hamd Al-Essa Organ Transplant Center of Kuwait, Kuwait.

Hemophagocytic syndrome combines febrile hepatosplenomegaly, pancytopenia, hypofibrinemia, and hepatic dysfunction. It is characterized by bone marrow and organ infiltration of activated, nonmalignant macrophages that phagocytize blood cells. It is rare among renal transplant recipients. Read More

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http://dx.doi.org/10.6002/ect.MESOT2018.P67DOI Listing
January 2019

Management of Chronic Active Antibody-Mediated Rejection in Renal Transplant Recipients: Single-Center Experience.

Exp Clin Transplant 2019 Jan;17(Suppl 1):113-119

From the Kuwait Ministry of Health, Hamed Al-Essa Organ Transplant Center, Sabah area, Kuwait.

Objectives: Data on the management of chronic antibody-mediated rejection after kidney transplantation are limited. We aimed to assess the impact of treatment of biopsy-proven chronic active antibodymediated rejection with combined plasma exchange, intravenous immunoglobulin, and rituximab treatment versus intravenous immunoglobulin alone or conservative management on the evolution of renal function in renal transplant recipients.

Materials And Methods: In this retrospective study, we compared patients diagnosed with chronic active antibody-mediated rejection who were treated with standard of care steroids, intravenous immunoglobulin, plasma exchange, and rituximab (n = 40) at our center versus those who received intravenous immunoglobulin only or just intensified maintenance immunosuppression (n = 28). Read More

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http://dx.doi.org/10.6002/ect.MESOT2018.O58DOI Listing
January 2019

Efficacy of 2 Doses of Rituximab on B-Cell and Antidonor Antibody and Outcomes of ABO-Incompatible Living-Donor Pediatric Kidney Transplant.

Exp Clin Transplant 2019 Jan;17(Suppl 1):105-109

From the Department of Nephrology and the Department of Pediatric Nephrology, Toho University, Faculty of Medicine, Tokyo, Japan.

Objectives: Rituximab treatment strategies vary in ABOincompatible pediatric kidney transplant recipients. Here, we present the efficacy of 2 doses of rituximab and subsequent outcomes in ABO-incompatible pediatric kidney transplant patients.

Materials And Methods: Our study of ABO-incompatible pediatric kidney transplant recipients included 21 who were pretreated with desensitization that included 2 doses of 100 mg rituximab (rituximab group) at 10 and 1 day pretransplant and 14 who received splenectomy without rituximab (splenectomy group). Read More

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http://dx.doi.org/10.6002/ect.MESOT2018.O43DOI Listing
January 2019

Renal Transplant and Its Outcomes: Single-Center Experience From India.

Exp Clin Transplant 2019 Jan;17(Suppl 1):78-82

From the Department of Nephrology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Objectives: Improvements in early graft survival and long-term graft function have made kidney transplant a more cost-effective alternative to dialysis. We aimed to assess renal transplant outcomes over a 9-month follow-up of recipients in a cost-limited setting (a tertiary care center in India).

Materials And Methods: Included patients in this prospective observational study were those who underwent renal transplant from July 2016 to February 2017 (8 months) and followed for 9 months. Read More

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http://dx.doi.org/10.6002/ect.MESOT2018.O14DOI Listing
January 2019

Living-Donor Kidney Transplant With Preformed Donor-Specific Antibodies.

Exp Clin Transplant 2019 Jan;17(Suppl 1):43-49

From the Department of Nephrology, Toho University, Faculty of Medicine, Tokyo, Japan.

Objectives: We investigated outcomes in living-donor kidney transplant recipients with preformed donor-specific antibodies (detected with flow cytometry and specified with the LABScreen single antigen test) under desensitization pretransplant and immunosuppression posttransplant.

Materials And Methods: Of 15 recipients included, 8 had ABO-incompatible kidney transplant. Six patients had sensitization caused by pregnancy, 8 by blood transfusion, 5 by previous transplants, and 1 by unknown cause. Read More

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http://dx.doi.org/10.6002/ect.MESOT2018.L42DOI Listing
January 2019

Correlation of Cytomegalovirus (CMV) Disease Severity and Mortality With CMV Viral Burden in CMV-Seropositive Donor and CMV-Seronegative Solid Organ Transplant Recipients.

Open Forum Infect Dis 2019 Feb 14;6(2):ofz003. Epub 2019 Jan 14.

Department of Medicine and the Division of Geographic Medicine and Infectious Disease, Tufts Medical Center, and Tufts University School of Medicine, Boston, Masschusetts.

Background: The rate of cytomegalovirus (CMV) viral load increase and peak viral loads are associated with CMV disease in kidney and liver transplant recipients, but relationships to disease severity or mortality have not been shown.

Methods: Using stored serial serum specimens from renal (n = 59) and liver (n = 35) transplant recipients (D+R-; CMV-seropositive donors, CMV-seronegative recipients) from 2 prospective, randomized, controlled, interventional prophylaxis trials of CMV immune globulin (CMVIG), CMV viral load was measured using the COBAS quantitative polymerase chain reaction assay and the World Health Organization CMV standard. Patients with severe CMV-associated disease were classified according to trial definitions. Read More

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https://academic.oup.com/ofid/article/doi/10.1093/ofid/ofz00
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http://dx.doi.org/10.1093/ofid/ofz003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366655PMC
February 2019
1 Read

Clinical differentiation of infectious mononucleosis that is caused by Epstein-Barr virus or cytomegalovirus: A single-center case-control study in Japan.

J Infect Chemother 2019 Feb 15. Epub 2019 Feb 15.

Department of General Medicine and Emergency Care, Toho University School of Medicine, Japan.

Introduction: Infectious mononucleosis (IM) is a common viral infection that typically causes fever, pharyngitis, and lymphadenopathy in young patients. The Epstein-Barr virus (EBV) is the most common cause of IM, followed by cytomegalovirus (CMV). Given that serological testing is associated with limitations regarding its accuracy, availability, and time to receive results, clinical differentiation based on symptoms, signs, and basic tests would be useful. Read More

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http://dx.doi.org/10.1016/j.jiac.2019.01.012DOI Listing
February 2019

Adjuvant therapy of cytomegalovirus IgM + ve associated biliary atresia: Prima facie evidence of effect.

J Pediatr Surg 2019 Jan 22. Epub 2019 Jan 22.

Department of Paediatric Surgery, Denmark Hill, London, UK SE5 9RS; Department of Hepatology Kings College Hospital, Denmark Hill, London, UK SE5 9RS. Electronic address:

Aim Of Study: CMV-IgM + ve associated biliary atresia (CMV-BA) is a distinct etiological subgroup characterized by older age at presentation and a greater degree of inflammation and hepatic fibrosis, leading to a worse outcome. We report our experience with adjuvant antiviral therapy after Kasai portoenterostomy (KPE).

Methods: Single-center prospective database identification of CMV-IgM + ve associated BA managed between 2003 and 2017. Read More

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http://dx.doi.org/10.1016/j.jpedsurg.2018.12.014DOI Listing
January 2019

Diagnosis and Management of CMV Colitis.

Curr Infect Dis Rep 2019 Feb 15;21(2). Epub 2019 Feb 15.

Pediatric Gastroenterology Unit, Pediatrics Department, "Dana-Dwek" Children's Hospital, Tel Aviv Sourasky Medical Center, 6 Weizman Street, 6423906, Tel Aviv, Israel.

Purpose Of Review: Cytomegalovirus (CMV) colitis is a relatively common end-organ infectious complication in immunocompromised hosts which negatively affects clinical outcomes. This paper presents the contemporary approaches to the diagnosis and management of CMV colitis and discusses some of the controversies of this condition, focusing on methods of diagnosis.

Recent Findings: While certain risk factors for CMV colitis are well recognized, the clinical as well as endoscopic features of this condition are nonspecific. Read More

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http://dx.doi.org/10.1007/s11908-019-0664-yDOI Listing
February 2019

[Experience of application of the PCR for identification of the causative agent of community acquired pneumonia in the military.]

Klin Lab Diagn 2018 ;63(10):641-645

«1586 Military Clinical Hospital» of the Ministry of Defense of the Russian Federation, 603105, Nizhniy Novgorod, Russian Federation.

Samples of sputum, blood, bronchoalveolar lavage, swabs from the oropharynx from 255 military personnel undergoing in-patient treatment with an x-ray confirmed diagnosis of community-acquired pneumonia (CAP) were examined by polymerase chain reaction (PCR). The comparison group was included 270 healthy recruits. The detection of S. Read More

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http://dx.doi.org/10.18821/0869-2084-2018-63-10-641-645DOI Listing
January 2018
5 Reads

A Prospective Multi-Center Observational Study of Cell-Mediated Immunity as a Predictor for Cytomegalovirus Infection in Kidney Transplant Recipients.

Am J Transplant 2019 Feb 15. Epub 2019 Feb 15.

Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, United States.

T-cell immunity is essential for the control of cytomegalovirus (CMV) infection post-transplant. We evaluated a CMV-specific peptide-based ELISPOT assay to determine whether assay results could predict subsequent CMV events. Adult kidney transplant recipients at 43 centers underwent ELISPOT testing to enumerate IFN-γ binding spot forming units (sfu) after stimulation of cells with an overlapping peptide pool of CMV pp65 and IE-1 proteins at the end of antiviral prophylaxis (EOP) and various timepoints thereafter. Read More

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http://dx.doi.org/10.1111/ajt.15315DOI Listing
February 2019

Cytomegalovirus infection among Human Immunodeficiency Virus (HIV) infected individuals on highly active anti-retroviral therapy in North-Central Nigeria.

Afr Health Sci 2018 Dec;18(4):1057-1065

Virology Unit, Department of Microbiology, University of Ilorin, P.M.B 1515 Ilorin-Nigeria.

Background: Cytomegalovirus (CMV) infection is common among HIV-infected individuals. Its contribution to morbidity and mortality became more apparent following introduction of highly active anti-retroviral therapy (HAART) which improved survival among HIV-infected individuals.

Objectives: This study aimed at determining the prevalence of both active and latent CMV infections among HIV-infected individuals on HAART in Ilorin, Nigeria. Read More

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http://dx.doi.org/10.4314/ahs.v18i4.27DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354892PMC
December 2018

PTX3 Polymorphisms Influence Cytomegalovirus Reactivation After Stem-Cell Transplantation.

Front Immunol 2019 31;10:88. Epub 2019 Jan 31.

Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.

Reactivation of latent human cytomegalovirus (CMV) in patients undergoing allogeneic stem-cell transplantation (HSCT) predisposes to several clinical complications and is therefore a major cause of morbidity and mortality. Although pentraxin-3 (PTX3) has been previously described to bind both human and murine CMV and mediate several host antiviral mechanisms, whether genetic variation in the locus influences the risk of CMV infection is currently unknown. To dissect the contribution of genetic variation within to the development of CMV infection, we analyzed described loss-of-function variants at the locus in 394 recipients of HSCT and their corresponding donors and assessed the associated risk of CMV reactivation. Read More

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http://dx.doi.org/10.3389/fimmu.2019.00088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365436PMC
January 2019

Role of Immunogenetics in the Outcome of HCMV Infection: Implications for Ageing.

Int J Mol Sci 2019 Feb 5;20(3). Epub 2019 Feb 5.

Department of Microbiology and Immunology, Medical University of South Carolina, 171 Ashley Ave, Charleston, SC 29425, USA.

The outcome of host-virus interactions is determined by a number of factors, some related to the virus, others to the host, such as environmental factors and genetic factors. Therefore, different individuals vary in their relative susceptibility to infections. Human cytomegalovirus (HCMV) is an important pathogen from a clinical point of view, as it causes significant morbidity and mortality in immunosuppressed or immunosenescent individuals, such as the transplanted patients and the elderly, respectively. Read More

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http://dx.doi.org/10.3390/ijms20030685DOI Listing
February 2019

[Prophylaxis of Recurrent Cytomegalovirus Uveitis with Topical Ganciclovir].

Klin Monbl Augenheilkd 2019 Feb 14. Epub 2019 Feb 14.

Augenklinik, UniversitätsSpital Zürich, Universität Zürich, Schweiz.

Purpose: Cytomegalovirus (CMV) can cause recurrent or chronic hypertensive anterior uveitis in immunocompetent patients. Antiviral treatment options include topical ganciclovir or systemic valganciclovir. However, recurrences are common after stopping treatment. Read More

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http://www.thieme-connect.de/DOI/DOI?10.1055/a-0816-5598
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http://dx.doi.org/10.1055/a-0816-5598DOI Listing
February 2019
2 Reads

HCMV latency: what regulates the regulators?

Med Microbiol Immunol 2019 Feb 14. Epub 2019 Feb 14.

Department of Medicine, University of Cambridge, Box 157 Addenbrooke's Hospital, Hills Road, Cambridge, CB2 0QQ, UK.

Human cytomegalovirus (HCMV) latency and reactivation is regulated by the chromatin structure at the major immediate early promoter (MIEP) within myeloid cells. Both cellular and viral factors are known to control this promoter during latency, here we will review the known mechanisms for MIEP regulation during latency. We will then focus on the virally encoded G-protein coupled receptor, US28, which suppresses the MIEP in early myeloid lineage cells. Read More

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http://dx.doi.org/10.1007/s00430-019-00581-1DOI Listing
February 2019

Cytokine-Mediated Induction and Regulation of Tissue Damage During Cytomegalovirus Infection.

Front Immunol 2019 29;10:78. Epub 2019 Jan 29.

Division of Infection and Immunity/Systems Immunity University Research Institute, Cardiff, United Kingdom.

Human cytomegalovirus (HCMV) is a β-herpesvirus with high sero-prevalence within the human population. Primary HCMV infection and life-long carriage are typically asymptomatic. However, HCMV is implicated in exacerbation of chronic conditions and associated damage in individuals with intact immune systems. Read More

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http://dx.doi.org/10.3389/fimmu.2019.00078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362858PMC
January 2019

Anticytomegalovirus Peptides Point to New Insights for CMV Entry Mechanisms and the Limitations of Screenings.

mSphere 2019 Feb 13;4(1). Epub 2019 Feb 13.

Department of Microbiology, The University of Tennessee, Knoxville, Tennessee, USA

Human cytomegalovirus (HCMV) is a ubiquitous betaherpesvirus that can cause severe disease following exposure, during primary infection, or latent virus reactivation in immunocompromised populations. These complications lead to a 1- to 2-billion-dollar economic burden, making vaccine development and/or alternative treatments a high priority. Current treatments for HCMV include nucleoside analogues such as ganciclovir (GCV), foscarnet, and cidofovir. Read More

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http://dx.doi.org/10.1128/mSphere.00586-18DOI Listing
February 2019

The IκB kinases restrict human cytomegalovirus infection.

J Virol 2019 Feb 13. Epub 2019 Feb 13.

Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York, United States of America

Human Cytomegalovirus (HCMV) is a ubiquitous herpesvirus that causes disease in immunosuppressed populations. HCMV has a complex relationship with innate immune signaling pathways. Specifically, HCMV has been found to block some aspects of inflammatory signaling while benefiting from others. Read More

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http://jvi.asm.org/lookup/doi/10.1128/JVI.02030-18
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http://dx.doi.org/10.1128/JVI.02030-18DOI Listing
February 2019
2 Reads

Volumetric MRI Study of the Brain in Fetuses with Intrauterine Cytomegalovirus Infection and Its Correlation to Neurodevelopmental Outcome.

AJNR Am J Neuroradiol 2019 Feb;40(2):353-358

From the Antenatal Diagnostic Unit (A.G., E.K., D.H., R.B., S.L.), Department of Obstetrics and Gynecology.

Background And Purpose: In recent years, effort has been made to study 3D biometry as a method for fetal brain assessment. In this study, we aimed to compare brain volumes of fetuses with cytomegalovirus infection and noninfected controls. Also, we wanted to assess whether there is a correlation to their neurodevelopmental outcome as observed after several years. Read More

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http://dx.doi.org/10.3174/ajnr.A5948DOI Listing
February 2019

Delayed IL-21 treatment preferentially expands peptide-specific CD8 T cells by reducing bystander activation of T cells.

Immunotherapy 2019 Feb 14. Epub 2019 Feb 14.

Biomedicine Production Branch, National Cancer Center, Goyang, 10408 Korea.

Aim: We previously reported a simple and practical procedure to generate peptide-specific CD8 T cells using peptide and IL-2, which is applied to produce human telomerase reverse transcriptase (hTERT)-specific CD8+ T cells for clinical use. We have modified the procedure to enhance the amplification of peptide-specific CD8 T cells adding IL-21.

Materials & Methods: Using human leukocyte antigen (HLA)-A*0201-restricted cytomegalovirus/pp65-specific CD8 T cells of healthy volunteers, we optimized the culture conditions by adjusting the dose and timing of IL-21 treatment. Read More

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http://dx.doi.org/10.2217/imt-2018-0095DOI Listing
February 2019
1 Read

Infectious complications and NK cell depletion following daratumumab treatment of Multiple Myeloma.

PLoS One 2019 13;14(2):e0211927. Epub 2019 Feb 13.

Division of Hematology, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden.

Treatment with Daratumumab (Dara), a monoclonal anti-CD38 antibody of IgG1 subtype, is effective in patients with multiple myeloma (MM). However, Dara also impairs the cellular immunity, which in turn may lead to higher susceptibility to infections. The exact link between immune impairment and infectious complications is unclear. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0211927PLOS
February 2019
1 Read

Joint association of carrying HLA-B*13:01 gene and human herpesvirus-6 with occupational trichloroethylene hypersensitivity syndrome.

Int Arch Occup Environ Health 2019 Feb 13. Epub 2019 Feb 13.

Poisoning Detection Center, Shenzhen Prevention and Treatment Center for Occupational Diseases, Shenzhen, China.

Purpose: Occupational trichloroethylene hypersensitivity syndrome (OTHS) clinically manifests as generalized severe rash resembling drug-induced hypersensitivity syndrome (DIHS) and afflicts predominantly HLA-B*13:01 gene carriers after their exposure to trichloroethylene. Meanwhile, OTHS may also be associated with human herpesvirus such as herpesvirus-6 (HHV6) and cytomegalovirus (HCMV) reported to participate in the pathology of DIHS. This study explored the association of carrying HHV6 and HCMV, and the joint association of carrying HLA-B*13:01 and HHV6 and HCMV with OTHS. Read More

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http://dx.doi.org/10.1007/s00420-019-01417-4DOI Listing
February 2019

Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1β Protein during Virion-Mediated Activation of the AIM2 Inflammasome.

MBio 2019 Feb 12;10(1). Epub 2019 Feb 12.

Vaccine and Gene Therapy Institute, Oregon Health and Science University, Portland, Oregon, USA

Secretion of interleukin-1β (IL-1β) represents a fundamental innate immune response to microbial infection that, at the molecular level, occurs following activation of proteolytic caspases that cleave the immature protein into a secretable form. Human cytomegalovirus (HCMV) is the archetypal betaherpesvirus that is invariably capable of lifelong infection through the activity of numerous virally encoded immune evasion phenotypes. Innate immune pathways responsive to cytoplasmic double-stranded DNA (dsDNA) are known to be activated in response to contact between HCMV and host cells. Read More

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http://dx.doi.org/10.1128/mBio.02510-18DOI Listing
February 2019
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Nucleotide Resolution Comparison of Transcription of Human Cytomegalovirus and Host Genomes Reveals Universal Use of RNA Polymerase II Elongation Control Driven by Dissimilar Core Promoter Elements.

MBio 2019 Feb 12;10(1). Epub 2019 Feb 12.

Department of Biochemistry, The University of Iowa, Iowa City, Iowa, USA

The large genome of human cytomegalovirus (HCMV) is transcribed by RNA polymerase II (Pol II). However, it is not known how closely this betaherpesvirus follows host transcriptional paradigms. We applied PRO-Seq and PRO-Cap methods to profile and quantify transcription initiation and productive elongation across the host and virus genomes in late infection. Read More

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http://dx.doi.org/10.1128/mBio.02047-18DOI Listing
February 2019

Disseminated CMV infection and HLH in a patient with well-controlled HIV and ulcerative colitis.

BMJ Case Rep 2019 Feb 11;12(2). Epub 2019 Feb 11.

Department of Genitourinary Medicine and HIV, Imperial College Healthcare NHS Trust, London, UK.

We present a case of haemophagocytic lymphohistiocytosis (HLH) in the context of disseminated cytomegalovirus (CMV) viraemia in a 50-year-old man with well-controlled HIV infection and ulcerative colitis (UC), for which he was receiving azathioprine. Peak CMV viral load was 371 000 copies/ml with evidence of end-organ CMV in the lungs and colon. A bone marrow biopsy showed evidence of haemophagocytosis of platelets, neutrophils and erythrocytes. Read More

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http://dx.doi.org/10.1136/bcr-2018-227916DOI Listing
February 2019
1 Read

Human bone marrow mesenchymal stem cells functionalized by hybrid baculovirus-adeno-associated viral vectors for targeting hypopharyngeal carcinoma.

Stem Cells Dev 2019 Feb 12. Epub 2019 Feb 12.

Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Department of Otolaryngology , No. 197, Ruijin 2nd Road , Shanghai, China , 200025 ;

Hypopharyngeal carcinoma is a common malignant tumor of the head and neck with a very poor prognosis; the median survival time for curatively treated patients was 17.2 months in India. However, cell-based gene therapy holds promise to improve patient outcomes. Read More

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http://dx.doi.org/10.1089/scd.2018.0252DOI Listing
February 2019
2 Reads

Incidence, risk factors, and outcome of BK polyomavirus infection after kidney transplantation.

World J Clin Cases 2019 Feb;7(3):270-290

Renal Transplantation, Barts Health NHS Trust, Royal London Hospital, London E1 1BB, United Kingdom.

Background: Polyomavirus-associated nephropathy is a leading cause of kidney allograft failure. Therapeutic options are limited and prompt reduction of the net state of immunosuppression represents the mainstay of treatment. More recent application of aggressive screening and management protocols for BK-virus infection after renal transplantation has shown encouraging results. Read More

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http://dx.doi.org/10.12998/wjcc.v7.i3.270DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369392PMC
February 2019
1 Read

First Onset Herpesviral Infection and Lung Injury in Allogeneic Hematopoietic Cell Transplantation.

Am J Respir Crit Care Med 2019 Feb 11. Epub 2019 Feb 11.

Univ Michigan, Internal Medicine, Ann Arbor, Michigan, United States ;

Rationale: "Non-infectious" pulmonary complications are significant causes of morbidity and mortality post allogeneic hematopoietic cell transplant. Early onset viral reactivations or infections are common after transplant. Whether the first onset viral infection causes "non-infectious" pulmonary complications is unknown. Read More

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http://dx.doi.org/10.1164/rccm.201809-1635OCDOI Listing
February 2019
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[A prognostic value of level of antiviral antibodies in the development of recurrence of bladder cancer].

Urologiia 2018 Dec(6):86-94

Department of Urology and Surgical Andrology of RMANPO, Moscow, Russia.

Introduction: Serological diagnosis of virus-associated tumors attracts the attention of many specialists. The changes in the level of antiviral antibodies in tumors of different localizations are proved. In some cases, the authors suggest using these data either for screening of tumors or for controlling the cure. Read More

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December 2018
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Pulse corticosteroids in treatment of rheumatic disease concomitant with cytomegalovirus infection.

Int J Rheum Dis 2019 Feb 10. Epub 2019 Feb 10.

Department of Rheumatology and Immunology, Peking University People's Hospital, Beijing Key Laboratory for Rheumatism Mechanism and Immune Diagnosis (BZ0135), Peking-Tsinghua Center for Life Sciences, Beijing, China.

Aim: To investigate the impact of corticosteroids on the outcome of antiviral therapy in rheumatic patients with cytomegalovirus (CMV)-emia.

Method: Sixty-two patients with rheumatic disease complicated by CMV infection from 2011 to 2014 were retrospectively analyzed.

Results: Fifty-five of 62 patients were diagnosed with CMV-DNAemia. Read More

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http://dx.doi.org/10.1111/1756-185X.13467DOI Listing
February 2019

In-hospital mortality of hematopoietic stem cell transplantation among children with nonmalignancies: A nationwide study in the United States from 2000 to 2012.

Pediatr Blood Cancer 2019 Feb 10:e27626. Epub 2019 Feb 10.

Division of Allergy and Immunology, Department of Pediatrics, SUNY Downstate Medical Center, Brooklyn, New York.

Background: Hematopoietic stem cell transplant (HSCT) can cure or alleviate a wide range of nonmalignant childhood conditions. However, few studies have examined longitudinal national trends of frequency or short-term complications of HSCT before 2006 when an HSCT became a reportable procedure by US law. By using a US nationally representative database, we conducted nationwide longitudinal analyses on demographics, in-hospital mortality, and short-term complications in nonmalignant HSCT from 2000 to 2012. Read More

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http://dx.doi.org/10.1002/pbc.27626DOI Listing
February 2019
1 Read

Congenital cytomegalovirus: Impact on child health.

Authors:
Mark R Schleiss

Contemp Pediatr 2018 Jul;35(7):16-24

Division of Pediatric Infectious Diseases and Immunology, Department of Pediatrics, University of Minnesota Medical School, 2001 6 Street SE, Minneapolis, Minnesota 55455, , ,

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368255PMC

Solitary ascending colon ulcer diagnosed as gastrointestinal CMV disease.

BMJ Case Rep 2019 Feb 9;12(2). Epub 2019 Feb 9.

Gastroenterology, University of Florida College of Medicine, Gainesville, Florida, USA.

A 42-year-old woman with a history of cholangiocarcinoma on adjuvant chemotherapy with capecitabine presented with painless haematochezia. She was found to have an isolated twenty-five mm ulcer in the ascending colon. Biopsies of the ulceration demonstrated typical cytomegalovirus (CMV) inclusions and her peripheral blood CMV PCR was significantly elevated. Read More

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http://dx.doi.org/10.1136/bcr-2018-226355DOI Listing
February 2019
2 Reads

The isoquinoline alkaloid berberine inhibits human cytomegalovirus replication by interfering with the viral Immediate Early-2 (IE2) protein transactivating activity.

Antiviral Res 2019 Feb 8;164:52-60. Epub 2019 Feb 8.

Department of Molecular Medicine, University of Padua, 35121, Padua, Italy. Electronic address:

The identification and validation of new small molecules able to inhibit the replication of human cytomegalovirus (HCMV) remains a priority to develop alternatives to the currently used DNA polymerase inhibitors, which are often burdened by long-term toxicity and emergence of cross-resistance. To contribute to this advancement, here we report on the characterization of the mechanism of action of a bioactive plant-derived alkaloid, berberine (BBR), selected in a previous drug repurposing screen expressly devised to identify early inhibitors of HCMV replication. Low micromolar concentrations of BBR were confirmed to suppress the replication of different HCMV strains, including clinical isolates and strains resistant to approved DNA polymerase inhibitors. Read More

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http://dx.doi.org/10.1016/j.antiviral.2019.02.006DOI Listing
February 2019

Evaluation of the Abbott ARCHITECT™ cytomegalovirus IgM/IgG, rubella IgM/IgG, and syphilis treponemal antibodies enzyme immunoassays in a mother and child health center population.

Diagn Microbiol Infect Dis 2019 Jan 17. Epub 2019 Jan 17.

Université de Montréal, 2900 Boulevard Édouard-Montpetit, Montréal, Québec, Canada, H3T 1J4; Centre Hospitalier Universitaire Sainte-Justine, 3175 Chemin de la Côte Sainte-Catherine, Montreal, Quebec, Canada, H3T 1C4.

This study evaluated the concordance of Architect™ chemiluminescent microparticle immunoassays with Captia™ ELISA for cytomegalovirus (CMV) IgM and IgG, with Enzygnost™ and Captia™ ELISA for rubella IgM and IgG and with Trep-Sure™ ELISA for syphilis treponemal antibodies in a mixed pediatric and obstetrical population. Total agreement between assays and Kappa statistic value were 82.5% (95% CI: 75. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07328893193002
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http://dx.doi.org/10.1016/j.diagmicrobio.2018.12.017DOI Listing
January 2019
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The Women and Children's Infectious Diseases Center: An integrated approach to congenital infectious diseases.

Clin Invest Med 2019 Jan 30;41(4):E211-E212. Epub 2019 Jan 30.

Department of Pediatrics, Faculty of Medicine, Université de Montréal, Montréal, QC.

Congenital infectious diseases, transmitted during the course of pregnancy, are estimated to affect nearly one in every hundred births worldwide. These infections may be associated with fetal and infant adverse health outcomes, due to congenital malformations caused by in utero transmission of the infectious organism itself (as is the case with cytomegalovirus, toxoplasmosis, syphilis and Zika virus), or due to chronic infection in the infant (as is the case with human immunodeficiency virus [HIV] and hepatitis B and C). In addition, children who are exposed, yet uninfected, may still suffer from the consequences of exposure to infectious pathogens or to the drugs given to treat pregnant women and prevent in utero transmission (as may be the case with HIV infection). Read More

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http://dx.doi.org/10.25011/cim.v41i4.32223DOI Listing
January 2019
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Spontaneously-resolving episodes of Cytomegalovirus DNAemia in allogeneic hematopoietic stem cell transplant recipients: virological features and clinical outcomes.

J Med Virol 2019 Feb 8. Epub 2019 Feb 8.

Microbiology Service, Hospital Clínico Universitario, INCLIVA Research Institute, Valencia, Spain.

It has been reported that low plasma cytomegalovirus (CMV) DNA loads are associated with an increased risk of overall mortality in allogeneic hematopoietic stem cell transplantation (allo-HSCT). Utilizing a conservative strategy for initiation of preemptive antiviral therapy (PET) (>1,500 IU/ml), we characterized the virological features of spontaneously-resolving episodes of CMV DNAemia and assessed their impact on mortality through the first year after transplantation. We reviewed the CMV DNA PCR results and clinical charts of 230 consecutive adult patients who underwent T-cell replete allo-HSCT at our center. Read More

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http://dx.doi.org/10.1002/jmv.25426DOI Listing
February 2019
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Transduction of Adeno-Associated Virus Vectors Targeting Hair Cells and Supporting Cells in the Neonatal Mouse Cochlea.

Front Cell Neurosci 2019 24;13. Epub 2019 Jan 24.

ENT Institute and Otorhinolaryngology Department, Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China.

Adeno-associated virus (AAV) is the preferred vector for gene therapy of hereditary deafness, and different viral serotypes, promoters and transduction pathways can influence the targeting of AAV to different types of cells and the expression levels of numerous exogenous genes. To determine the transduction and expression patterns of AAV with different serotypes or promoters in hair cells and supporting cells in the neonatal mouse cochlea, we examined the expression of enhanced green fluorescent protein (eGFP) for five different types of AAV vectors [serotypes 2, 9, and Anc80L65 with promoter cytomegalovirus (CMV)-beta-Globin and serotypes 2 and 9 with promoter chicken beta-actin (CBA)] in cochlear explant cultures and we tested the transduction of AAV2/2-CBA, AAV2/9-CBA, and AAV2/Anc80L65-CMV by microinjection into the scala media of the cochlea. We found that each AAV vector had its own transduction and expression characteristics in hair cells and supporting cells in different regions of the cochlea. Read More

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http://dx.doi.org/10.3389/fncel.2019.00008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353798PMC
January 2019

HCMV trimer- and pentamer-specific antibodies synergize for virus neutralization but do not correlate with congenital transmission.

Proc Natl Acad Sci U S A 2019 Feb 7. Epub 2019 Feb 7.

Department of Molecular Microbiology & Immunology, Oregon Health & Science University, Portland, OR 97239;

Human cytomegalovirus (HCMV) causes substantial disease in transplant patients and harms the development of the nervous system in babies infected in utero. Thus, there is a major focus on developing safe and effective HCMV vaccines. Evidence has been presented that a major target of neutralizing antibodies (NAbs) is the HCMV pentamer glycoprotein gH/gL/UL128-131. Read More

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http://dx.doi.org/10.1073/pnas.1814835116DOI Listing
February 2019
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Cytomegalovirus host entry and spread.

J Gen Virol 2019 Feb 7. Epub 2019 Feb 7.

School of Chemistry and Molecular Biosciences and Child Health Research Centre, University of Queensland, Brisbane, Australia.

Cytomegaloviruses (CMVs) are large, complex pathogens that persistently and systemically colonize most mammals. Human cytomegalovirus (HCMV) causes congenital harm, and has proved hard to control. One problem is that key vaccine targets - virus entry and spread in naive hosts - remain ill-defined. Read More

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http://dx.doi.org/10.1099/jgv.0.001230DOI Listing
February 2019

Study of the mechanism for increased protein expression via transcription potency reduction of the selection marker.

Bioprocess Biosyst Eng 2019 Feb 7. Epub 2019 Feb 7.

Department of Cell Biol, Institute of Biomedicine & National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, 730 Building of Biology, 601 W Huangpu Ave, Guangzhou, 510630, Guangdong, People's Republic of China.

Stable transfection of mammalian cells using various expression cassettes for exogenous gene expression has been well established. The impact of critical factors in these cassettes, such as promoter and enhancer elements, on recombinant protein production in mammalian cells has been studied extensively to optimize the expression efficiency. However, few studies on the correlation between the strength of selection marker and the expression of gene of interest (GOI) have been reported. Read More

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http://dx.doi.org/10.1007/s00449-019-02083-zDOI Listing
February 2019

New short-term heat inactivation method of cytomegalovirus (CMV) in breast milk: impact on CMV inactivation, CMV antibodies and enzyme activities.

Arch Dis Child Fetal Neonatal Ed 2019 Feb 6. Epub 2019 Feb 6.

Institute of Medical Virology and Epidemiology of Viral Diseases, University Hospital of Tuebingen, Tubingen, Germany.

Objectives: Breast milk (BM) is the primary source of cytomegalovirus (CMV) transmission to premature infants with potentially harmful consequences. We therefore wanted to evaluate temperature and duration of short-term BM pasteurisation with respect to CMV inactivation, effect on CMV-IgG antibodies and BM enzyme activities.

Methods: 116 artificially CMV-spiked BM and 15 wild-type virus-infected samples were subjected for 5 s to different temperatures (55°C-72°C). Read More

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http://dx.doi.org/10.1136/archdischild-2018-316117DOI Listing
February 2019

Strategies of adoptive T -cell transfer to treat refractory viral infections post allogeneic stem cell transplantation.

J Hematol Oncol 2019 Feb 6;12(1):13. Epub 2019 Feb 6.

Department of Pediatric Hematology, Oncology, Hemostaseology and Stem Cell Transplantation, Dr. von Hauner University Children's Hospital, Ludwig Maximilian University Munich, Lindwurmstrasse 4, 80337, Munich, Germany.

Background: Allogeneic hematopoietic stem cell transplantation (HSCT) can expose patients to a transient but marked immunosuppression, during which viral infections are an important cause of morbidity and mortality. Adoptive transfer of virus-specific T cells is an attractive approach to restore protective T -cell immunity in patients with refractory viral infections after allogeneic HSCT.

Objectives: This narrative review summarizes clinical evidence and developments of almost 30 years of adoptive T -cell transfer. Read More

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http://dx.doi.org/10.1186/s13045-019-0701-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364410PMC
February 2019
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Dissociating nNOS (Neuronal NO Synthase)-CAPON (Carboxy-Terminal Postsynaptic Density-95/Discs Large/Zona Occludens-1 Ligand of nNOS) Interaction Promotes Functional Recovery After Stroke via Enhanced Structural Neuroplasticity.

Stroke 2019 Feb 7:STROKEAHA118022647. Epub 2019 Feb 7.

From the Institution of Stem Cells and Neuroregeneration, Nanjing Medical University, China (H.-Y.N., Y.-X.S., Y.-H.L., B.C., D.-L.W., Y.Z., J.D., H.-Y.L., K.X., T.-Y.L., L.C., H.-Y.W., C.-X.L., D.-Y.Z.).

Background and Purpose- Stroke is a major public health concern worldwide. Although clinical treatments have improved in the acute period after stroke, long-term therapeutics remain limited to physical rehabilitation in the delayed phase. This study is aimed to determine whether nNOS (neuronal NO synthase)-CAPON (carboxy-terminal postsynaptic density-95/discs large/zona occludens-1 ligand of nNOS) interaction may serve as a new therapeutic target in the delayed phase for stroke recovery. Read More

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http://dx.doi.org/10.1161/STROKEAHA.118.022647DOI Listing
February 2019
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