2,360 results match your criteria Cytology and Genetics [Journal]


Isolating and confirming the Mudr­inserted flanking sequences of maize.

Tsitol Genet 2017 Mar-Apr;51(2):89-90

MuDR exhibits the highest transposition activity and insertional mutagenesis frequency in Mutator (Mu) family. If we isolate the MuDR­insertion­specific flanking sequences (MuDRFs), it will be crucial for using Mu element­mediated mutants. The MuDR­TAIL­PCR system was constructed and optimized using a combination of MuDR­TIR­nested specific primers and 12 arbitrary degenerate (AD) primers, modified reaction system and procedure and mutant DNA templates of 87 genotypes from M2 or M2:3 families created by crossing the W22::Mu line (active MuDR donor parent) from the UniformMu population with the Zong31 (Z31) line (recipient parent). Read More

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November 2018
6 Reads

Induction of salt resistance of arabidopsis salicylatedeficient transformants NahG by influence of nitric oxide donor.

Tsitol Genet 2017 Mar-Apr;51(2):79-88

The influence of treatment with the donor of nitric oxide sodium nitroprusside (SNP – 0,5 mM) on the salt resistance of Arabidopsis plants of wild-type (Col-0) and transformant with the gene of bacterial salicylatehydroxylase (NahG) was compared. The basic resistance against the salt stress (200 mM of NaCl) in transformants NahG was higher. In the conditions of salt stress these plants differed in the higher activity of antioxidant enzymes and the raised content of carbohydrates and anthocyans. Read More

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November 2018
1 Read

Molecular characterization of Fusarium resistance from Elymus repens introgressed into bread wheat.

Tsitol Genet 2017 Mar-Apr;51(2):74-8

A cross was made of Elymus repens onto the wheat cultivar Crocus and BC1 progeny advanced to BC1F7 by single seed descent. Sixteen lines were selected based on agronomic performance and evaluated in an FHB epiphytotic nursery. Eight lines with FHB resistance were selected. Read More

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November 2018
2 Reads

Diversity of ukrainian winter common wheat varieties with respect to storage protein loci and molecular markers for disease resistance genes.

Tsitol Genet 2017 Mar-Apr;51(2):59-73

Diversity of Ukrainian winter common wheat varieties was studied with respect to the storage protein loci Gli-A1, Gli-B1, Gli-D1, Glu-A1, Glu-B1, Glu-D1, Gli-A3, Gli-B5, and Gli-A6 (362 varieties) and markers for the Lr34/Yr18/Pm38/Sr57/Bdv1 gene conferring moderate resistance to a number of biotrophic pathogens, the Tsn1 gene for sensitivity to the toxins A of the necrotrophic fungi Pyrenophora triticirepentis and Stagonospora nodorum, the Tsc2 gene for sensitivity to the toxin B of P. triticirepentis, and the TDF_076_2D gene for moderate resistance to Fusarium head blight (181 varieties). Significant differences in frequencies of alleles at these marker loci between groups of varieties developed in different soil and climatic zones were revealed. Read More

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November 2018
1 Read

Plants and microgravity: patterns of microgravity effects at the cellular and molecular levels.

Tsitol Genet 2017 Mar-Apr;51(2):47-58

An overview on the effects of real and simulated microgravity on certain cell components and processes, including new information obtained recently, is presented. Attention is focused on the influence of microgravity on the cytoplasmic membrane state, transcriptome and proteome, cell wall remodeling, and Ca2+-signaling in plant cells that are not specialised to gravity perception. It is emphasized the exceptional significance of the data on the organ-specific remodeling of the transcriptome and proteome in response to space flight, that discovers new advanced approaches to implement the fundamental and applied problems of plant space biology. Read More

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November 2018
1 Read

Cell genetic engineering – transmission genetics of plants.

Authors:
N Kuchuk

Tsitol Genet 2017 Mar-Apr;51(2):40-6

It has been described achievements of cell and genetic engineering that led to formation of new genetics chapter – transmission genetics. It has been analyzed results and showed new opportunities in the field of transgenomic somatic hybrids and cybrid obtaining, production of transgenic plants with agronomic pharmaceutical application, development of transplastomic plants, accu-mulation of recombinant proteins by using the transient expression of foreign genes in plants. Read More

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November 2018
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125 years of virology and ascentof biotechnologies based on viral expression.

Tsitol Genet 2017 Mar-Apr;51(2):19-39

The study of viruses lasts for more than a century since their discovery in 1892. In recent decades, viruses are also being actively exploited as a biotechnological tool. Plant-virus-driven transient expression of heterologous proteins is an actively developing production platform; it is the basis of several industrial processes that are currently being used for the production of multiple recombinant proteins. Read More

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November 2018
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Double helix of the journal «Сytology and genetics»: fity years later.

Authors:
Ya B Blume

Tsitol Genet 2017 Mar-Apr;51(2):3-8

This survey paper contains a brief analysis of publications included in current issue of scientific journal «Cytology and Genetics» dedicated to its 50th anniversary. These papers reflect scientific achievements of their authors in the field of genetics and cell biology and underine the potential of these two biological disciplines, forming «double helix» of the journal. Read More

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November 2018
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Morphogenesis in vitro in inbreds of maize Lancaster heterotic group.

Tsitol Genet 2017 Jan-Feb;51(1):69-75

The genotypic features of morphogenesis and regeneration in vitro for five maize inbreds of perspective breeding Lancaster heterotic group compared to representatives of others heterotic groups – PLS61, A188 and Chi31 were studied. It was identified that the ratio of such types of morphogenesis as organogenesis and embryoidogenesis in callus culture was determined by the explant genotype and the concentration of sucrose in the medium for callusogenesis. The frequency of embryoidogenesis as the most effective type of morphogenesis for further regeneration in Lancaster inbreds averaged about 40. Read More

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November 2018
9 Reads

THE EFFECTS OF COLCHICINEINDUCED AUTOTETRAPLOIDY ON SELECTED CHARACTERISTICS OF NURUOZAK (SALVIA LERIIFOLIA).

Tsitol Genet 2017 Jan-Feb;51(1):81-2

Nuruozak (Salvia leriifolia Benth), is a perennial herbaceous plant that is endemic to Iran and has recently been introduced as a medicinal plant. Artificial polyploidy is an efficient method to increase the production of secondary metabolites and can result in a whole spectrum of genetic, molecular and physiological modifications. In order to produce an autotetraploid population of nuruozak, various concentrations of colchicine (0. Read More

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November 2018
1 Read

Formaldehyde induces DNA strand breaks on spermatozoa and lymphocytes of Wistar rats.

Tsitol Genet 2017 Jan-Feb;51(1):78-80

Formaldehyde (FA) interacts with biological molecules such as DNA and it induces DNA-protein cross-links (DPCs), oxidative stress, reactive oxygen species (ROS), methylation, chromosomal damage, fragmentation, and adducts of DNA, which are considered the most important genotoxic effects caused by exposure to FA. The purpose of this study was to evaluate the percentage of DNA fragmentation on lymphocytes and spermatozoa from Wistar rats exposed to different doses of FA. The results about the percentage of fragmentation of DNA in lymphocytes and spermatozoa, were statistical different from controlled group versus treated groups respectively to (p < 0. Read More

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November 2018
2 Reads

A novel Alui-polymorphism in the fourth intron of the chicken growth hormone gene.

Tsitol Genet 2017 Jan-Feb;51(1):76-7

A novel AluI-polymorphism in the fourth intron of chicken growth hormone gene was shown. It was detected the cytosine to thymine transition in the restriction site for AluI. Primers, that flanking the 460 bp fragment of the fourth intron, containing a polymorphic restriction site for AluI, was designed. Read More

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November 2018
2 Reads

ISSR-PCR fingerprinting of Ukrainian sweet cherry (Prunus avium L.) cultivars.

Tsitol Genet 2017 Jan-Feb;51(1):61-8

ISSR-PCR markers were used to assess genetic diversity and to elucidate relatedness among 21 Ukrainian and 3 West European sweet cherry varieties, which are widely cultivated in Ukraine. The discriminatory potential was tested for 11 ISSR-PCR primers, which produced 193 amplicons. UBC 835, 836, 841 and 881 were identified as the best primers suitable for routine application. Read More

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November 2018
1 Read

Features inheritance of root system Arabidopsis thaliana (L.) Heynh. the interaction of genes CTR1 AND ALF3, NPH4 and IAR2.

Authors:
S G Hablak

Tsitol Genet 2017 Jan-Feb;51(1):41-50

The results of study interaction of genes CTR1, ALF3 and NPH4, IAR2 inheritance attributes of the root system Arabidopsis. It is set that there is a recession epistasis in the second generation of crossing of plants mutant lines ctr1-1 × alf3-1 (alf3-1 alf3-1 > CTR1_). At crossing of plants mutant lines nph4-1 × iar2-1 there is polymeric co-operation of genes NPH4 and IAR2 in the generation of F2. Read More

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November 2018
2 Reads

Time-course of pathogen induced accumulation of callose as mechanical protective barrier in wheat seedlings.

Tsitol Genet 2017 Jan-Feb;51(1):34-40

Results of fluorescence microscopic research and quantitative luminescent analysis of pathogen induced callose accumulation in winter wheat seedlings of two cultivars different in resistance to eye spot causal agent are presented. Higher content of constitutive callose in intact seedlings of unsusceptible cultivar at the initial stages of vegetation was determined. It correlates with resistance of this cultivar to the eye spot causal agent. Read More

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November 2018
1 Read

Degree of phenotypic dominance and heritability on the plant height character in wheat hybrids with different Rht-gene alleles.

Tsitol Genet 2017 Jan-Feb;51(1):25-33

The degree of dominance and the coefficient of heritability have been investigated as indicators of the allelic interaction at loci Rht8 and Rht-B1, which is characterized by the partial, complete or over dominance of greater plant height and by the low or medium heritability. Alleles with a weaker or without direct negative effect dominate under alleles reducing the plant height stronger. The impact of weather conditions on a change of the dominance is discussed. Read More

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November 2018
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Analysis of cellulose synthase gene expression strategies in higher plants using RNA-sequencing data.

Tsitol Genet 2017 Jan-Feb;51(1):12-24

The transcriptomes from different organs and tissues of western poplar, eucalyptus, soybean and common bean were studied. The expression level of cellulose synthase genes was notably different in different types of tissues and organs in studied plants. For common bean and eucalyptus transcriptome the domination of certain cellulose synthase genes was typical. Read More

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November 2018
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Establishment of transgenic lettuce plants producing potentially anti-hypertensive shRNA sequencing data.

Tsitol Genet 2017 Jan-Feb;51(1):3-11

Development of RNAi-based therapeutics is a fast growing field of pharmaceutical industry. Using plants for production of pharmaceutically valuable siRNAs may have significant advantages of cost-effectiveness, scalability and low risk of contamination with human pathogens. If edible plant species are genetically engineered to synthesize siRNAs, the costly stage of target product purification may be omitted. Read More

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November 2018
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Adaptation of the gymnosperms to the conditions of irradiation in Chornobyl zone: from morphological anomalies to the molecular genetic consequences.

Tsitol Genet 2016 Nov-Dec;50(6):80-9

It is known that the most suitable plant indicator targets for radiation pollution biomonitoring are conifers, because they have high radiosensitivity. In this review are briefly considered previously accumulated information about the genetic nature of morphological abnormalities in gymnosperms, induced by acute and chronic irradiation in the exclusion zone of the Chornobyl nuclear power plant. Since in the last decade appeared additional number of important research results, dedicated to the analysis of molecular biological and molecular genetic effects of chronic irradiation on the coniferous plants growing in the exclusion zone of the Chornobyl disaster, all these results are also analyzed in current review. Read More

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November 2018
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Systems biology is an efficient tool for investigation of low-dose chronic irradiation influence on plants in the Chernobyl zone.

Tsitol Genet 2016 Nov-Dec;50(6):60-79

The paper discusses different methodological approaches to the study of transgenerational alterations of metabolic pathways in soybean and flax seeds in the process of adaptation to chronic irradiation in the Chernobyl alienation zone. A combination of general biological methods and novel approaches, such as genomics, proteomics, cytogenetics, and mutagenesis, allows researchers to analyze an organism’s systemic response and identify the latent chronic irradiation effects in plants from the Chernobyl zone. The proteomic approaches are especially efficient, since they range from the identification of changes in abundance and folding of individual proteins to the characterization of posttranslational modifications, trends of qualitative changes during seed maturation, or protein-protein interactions during plant growth and development under permanent impacts of stress factors. Read More

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November 2018
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Long term effects of chernobyl contamination on dna repair function and plant resistance to biotic and abiotic stress factors.

Tsitol Genet 2016 Nov-Dec;50(6):34-59

Thirty years after the Chernobyl explosion we still lack information regarding the genetic effects of radionuclide contamination on the plant population. For example, are plants adapting to the low dose of chronic ionising irradiation and showing improved resistance to radiation damage? Are they coping with changing/increased pathogenicity of fungi and viruses in the Chernobyl exclusion zone? Are plant populations rapidly accumulating mutational load and should we expect rapid microevolutionary changes in plants in the Chernobyl area? This review will try to summarise the current knowledge on these aspects of plant genetics and ecology and draw conclusions on the importance of further studies in the area around Chernobyl. Read More

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November 2018
1 Read

Morphological evaluation of the effectiveness of bipolar radiofrequency ablation for thyroid nodules.

Tsitol Genet 2016 Nov-Dec;50(6):29-33

Recently, radiofrequency ablation has been increasingly used for the treatment of thyroid nodules. The widespread introduction of this method, however, hampered by the lack of data on efficacy and safety of different devices that are currently on the market, the selection of the optimal mode of procedure also remains to be elucidated. Experimental data obtained during the application of the original patented device is presented. Read More

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November 2018
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Influence of nitrate in drinking water on the prevalence of thyroid cancer and other diseases (literature review and experience in post-chernobyl period in Belarus).

Tsitol Genet 2016 Nov-Dec;50(6):23-8

In the last 60 years dramatically increased the content of nitrates in groundwater due to intensive use of nitrogen fertilizers in agriculture. Research in post-Chernobyl period has shown that a sharp increase in the incidence of thyroid cancer depends not only on the level of thyroid dose, but also on the level of nitrates in groundwater. Read More

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November 2018
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30 years of the Chernobyl accident. Molecular genetic mechanisms of carcinogenesis of thyroid gland.

Tsitol Genet 2016 Nov-Dec;50(6):15-22

The review presents data on the basic molecular ge-netic mechanisms of formation of papillary thyroid carcinoma. The participation of ionizing radiation in the cancer pathogenesis was analyzed. The role of tu-mor microenvironment, inflammation and nuclear trans-cription factor NF-κB in the initiation and development of papillary thyroid carcinoma was shown. Read More

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November 2018
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Experience of thyroid gland status screening in postchernobyl period.

Tsitol Genet 2016 Nov-Dec;50(6):8-14

The main results of thirty-year period of repeated screening programs and observational studies in contaminated and non-contaminated regions of Belarus are analyzed. The possible reasons of discrepant and contradictory data about the consequences of Chernobyl catastrophe delivered by different study groups during early period after the accident are discussed. High incidence of thyroid nodular disease and cancer in children and adolescence after the catastrophe are described. Read More

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November 2018
2 Reads

Chornobyl thirty years later: evaluation of the consequence by biologists and medics Experience of thyroid gland status screening in postchernobyl period.

Tsitol Genet 2016 Nov-Dec;50(6):3-7

Summarizing paper contains short analysis of the publications resulting cell biological, molecular genetic and population genetic investigations devoted to studying of accute radiation exposure and chronic radiation contamination effects on biological systems after disaster on Chornobyl nuclear power station in 1986. Read More

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November 2018
1 Read

IFNL4 polymorphism as a predictor of chronic hepatitis C treatment efficiency in Ukrainian patients.

Tsitol Genet 2016 Sep-Oct;50(5):79-84

The aim of this study was to examine association between IFNL4 gene ss469415590 and treatment efficiency in group of Ukrainian PEG-interferon/ribavirin-treated chronic hepatitis C patients. Study group consisted of 92 unrelated hepatitis C virus genotype 1 mono-infected patients: case group - 29 patients with late or absent virological response; control group - 63 patients with sustained virological response. Study material was genomic DNA. Read More

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December 2018
1 Read

Production of branched root hairs under progressive drought stress in Arabidopsis thaliana.

Authors:
J Bobrownyzky

Tsitol Genet 2016 Sep-Oct;50(5):72-8

In this study we presented data on the production of branched root hairs in the seedlings of A. thaliana under progressive water deficit. The overall production of branched hairs was quite high under stress conditions and amounted to 8,27 %. Read More

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December 2018
1 Read

Influence of cold on organization of actin filament in various cell types root Arabidopsis thaliana.

Tsitol Genet 2016 Sep-Oct;50(5):65-71

The effect of the low temperature (4 ºC) on the orga-nization of actin filaments (microfilaments) of cells of different growth zones of the root of Arabidopsis thaliana (L.) have been studied. It was found that cold treatment inhibits growth of the main root and gives its morphology, causing a large number of deformed (ectopic) root hairs in the zone of differentiation. Read More

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December 2018
1 Read

Association of polymorphisms in intron 2 of FGFR2 and breast cancer risk in Сhinese women.

Tsitol Genet 2016 Sep-Oct;50(5):59-64

Recent genome-wide association studies (GWAS) demonstrated that genetic variation in intron 2 of fibroblast growth factor receptor 2 (FGFR2) was a novel risk for breast cancer. We investigated whether two SNPs rs1219648 and rs2981582 in intron 2 of FGFR2 were associated with the risk of breast cancer in Chinese women. A total of 340 female breast cancer patients and 400 normal age-matched controls were recruited. Read More

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December 2018
1 Read

Association of the ace I/D gene polymorphism with DNA damage in hypertensive men.

Tsitol Genet 2016 Sep-Oct;50(5):48-58

The aim of the study was to evaluate the association between the angiotensin-converting enzyme ACE I/D (rs4340) polymorphism and DNA damage in pati-ents with essential hypertension (EH). The I/D polymorphism of ACE was determined by polymerase chain reaction in 170 male hypertensive patients and 64 normotensive blood donors. We used flow cytometry to determine the levels of cell death, micronuclei and accumulation of peripheral blood leukocytes in G1/G0, S, G2/M phases of the cell cycle. Read More

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December 2018
1 Read

Parathyroid hormone influence on chondrogenic progenitor cells differentiation.

Tsitol Genet 2016 Sep-Oct;50(5):43-7

The anabolic effects require brief exposures to higher than average PTH concentrations. The catabolic effects result from pathological conditions in which parathyroid glands secrete too much hormone continuously at a sustained level. The aim of the present study was observance of intermittent (I-PTH) and continuous (C-PTH) PTH 1-34 administration influence on bovine chondrogenic progenitor cells (CPC) differentiation. Read More

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December 2018
1 Read

Specific features of root aerenchyma formation in Sium latifolium L. (Apiaceae).

Tsitol Genet 2016 Sep-Oct;50(5):34-42

Types of aerenchyma formation in adventive roots of wild plant S. latifolium L. are described for the first time. Read More

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December 2018
1 Read

Anatomical abnormalities of the intertribal cybrid between Brassica napus and Lesquerella fendleri.

Tsitol Genet 2016 Sep-Oct;50(5):25-33

The anatomical research of the vegetative organs of the cytoplasmatic hybrid in vitro plants containing the Brassica napus nucleus and the Lesquerella fendleri chloroplasts had been conducted in comparison to the parental forms. It was found, that the anatomical structure of the cybrid had been similar to rapeseed. Anomalous changes in the epithelial, parenchymal and connective tissue of the leaf, stalk, stem, and root of the cybrid were detected. Read More

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December 2018
2 Reads

Loss of heterozygosity of individual loci in Arabidopsis thaliana regenerants cultured with para-fluorophenylalanine.

Tsitol Genet 2016 Sep-Oct;50(5):17-24

Precise chromosome segregation is vital for speciation and hybrid formation. The aim of the work was to study the chromosomes behaviour and inheritance of maternal and paternal genomes in Arabidopsis regenerants de-rived from in vitro cultured cells on the medium with PFFA. The Arabidopsis thaliana model hybrid between Columbia and Landsberg erecta ecotypes was developed, which chromosomes were easy to distinguish using the 12 SSLP selected markers. Read More

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December 2018
1 Read

Intra- and intertissular cytomictic interactions in mic-rosporogenesis of mono- and dicotyledonous plants.

Tsitol Genet 2016 Sep-Oct;50(5):3-16

A comparative cytological analysis of intra- and intertissular cytomictic interactions in early micro-sporogenesis of mono- and dicotyledonous plants was performed by the example of the two cellular systems - microsporocytes and tapetum. It is found that cytomixis is the component of intratissular interactions mainly. In the tapetum cells cytomixis is notable for structural and temporary taxon specific features. Read More

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December 2018
1 Read

Chromosomal DNA balance in human stem cell line 4BL.

Tsitol Genet 2016 Jul-Aug;50(4):79-89

In the previous cytogenetic study of new human stem cell line 4BL at the 205th passage we observed the ploidy of chromosomal set and regular aberrations. To investigate the nature of monosomy of certain chromosomes the array CGH and FISH analyses have been used. The aberrations of chromosomes have been identified in all the cases of monosomies previously revealed by G-banding. Read More

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December 2018
1 Read

A case of prenatal detection of a de novo unbalanced complex chromosomal rearrangement involving four chromosomes.

Tsitol Genet 2016 Jul-Aug;50(4):74-8

Complex chromosomal rearrangements are rarely observed prenatally. Genetic counceling of CCR carriers is complicated, especially in cases of de novo origin of the rearrangement. Here we present a new case of a de novo CCR involving four chromosomes observed in amniotic fluid cells of the fetus at 17 weeks of gestation. Read More

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December 2018
1 Read

Regulation of leucogenesis by extracellular ubiquitin in rodents after chemically induced inhibition.

Tsitol Genet 2016 Jul-Aug;50(4):69-73

To study the influence of intraperitoneally injected extracellular ubiquitin on regeneration of leucopoiesis calculation of nuclear cell count in bone marrow and peripheral blood smearsstained withazure-eosin was performed. In the first, control group of animals inhibition of haematopoiesis achieved by means of 100 mg/kg cyclophosphamide LD50 50–200 mg/kg injection. Bone marrow and peripheral blood samples from the first group of rats had been taken at 24, 48, 72, 96 and 168 h points after injection of cytostatic. Read More

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December 2018
1 Read

The polyphasic taxonomic analysis of the strain Bacillus sp. C6 – phytopathogenic microorganisms antagonist.

Tsitol Genet 2016 Jul-Aug;50(4):62-8

The polyphasic taxonomic analysis of the strain-antagonist of phytopathogenic bacteria and micromycetes was carried. It was found that the combination of culture-morphological, physiological and biochemical properties allowed to attribute the strain to the group Bacillus subtilis. It was shown that fatty acids of the cell walls of the strain were represented mainly by branched derivatives of iso- and anti iso- C15:0 and C17:0 fatty acids (85%) which was typical for the Bacillus amyloliquefaciens species. Read More

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December 2018
1 Read

Inhibiting effect of agmatine on genetically programmed death of leukocytes in experimental diabetes mellitus.

Tsitol Genet 2016 Jul-Aug;50(4):50-61

This study is an attempt to elucidate of agmatine effects upon leukocyte apoptosis in experimental diabetes mellitus (EDM). We demonstrated the increase in numbers of the leukocytes with both early and late signs of apoptosis at diabetes. Further changes in the morphofunctional state of the leukocytes include the increased amount of fragmented DNA, elevated apoptotic index and violated ratio of p53 to Bcl-2 proteins. Read More

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December 2018
1 Read

Immunoregulatory effects of vitamin D3 in experimental type 1 diabetes.

Tsitol Genet 2016 Jul-Aug;50(4):38-49

The mechanisms of diabetes-associated impairment of cellular immune defense and its regulation by vitamin D3 are not fully elucidated. The study was devoted to investigating the functional state of T-cell immunity as well as humoral immune activity in response to artificial immunization in experimental diabetes and after prolonged administration of vitamin D3. It was established that diabetes is characterized by a 2. Read More

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December 2018
12 Reads

Sr33 & Sr35 gene homolog indentification in genomes of cereals related with Aegilops tauschii & Triticum monococcum.

Tsitol Genet 2016 Jul-Aug;50(4):26-37

Using bioinformatics analysis, the homologues of the genes Sr33 and Sr35 were identifed in the genomes of Triticum aestivum, Hordeum vulgare and Triticum urartu. It is known that these genes provide resistance to hightly virulent wheat stem rust races (Ug99). To identify important for resistance amino acid sites, the comparison of the founded homologues with the Sr33 and Sr35 protein sequences was performed. Read More

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December 2018
1 Read

Differential expression of a cysteine proteinase and cystatin pair as side­by­side fusion forms in Escherichia coli.

Authors:
A Gholizadeh

Tsitol Genet 2016 Jul-Aug;50(4):16-25

As a basic study, the fusion expressions of two functionally related proteins were described. The side by side fusion construction, expression, purification and functional characterization of Arabidopsis papain-like cysteine proteinase (CP) and cysteine proteinase inhibitor (CPI) were successfully carried out by using an Escherichia coli expression system without affecting the recombinant bacterial growth. The purification products of two different fused constructs designated as «R1: H2N-maltose binding protein-CPI-CP-COOH and R2: H2N-maltose binding protein-CP-CPI-COOH» showed inverse enzymatic/inhibitory activities, in vitro. Read More

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December 2018
1 Read

Colocalization of USP1 and РН domain of Bcr­Abl oncoprotein in terms of cronic myeloid leukemia cell rearrangements.

Tsitol Genet 2016 Jul-Aug;50(4):11-5

The development of chronic myeloid leukemia (CML) is the result of a reciprocal translocation between chromosomes 9 and 22 due to the emergence of Philadelphia chromosome. The product of this mutation is a hybrid oncoprotein Bcr-Abl. According to the results of mass spectrometric analysis, USP1 protein was identified as a potential candidate for interaction with the PH domain Bcr-Abl oncoprotein. Read More

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December 2018
2 Reads

The influence of protein kinase KIN10 gene expression on root phenotype of Arabidopsis thaliana root system in condition of energy stress.

Tsitol Genet 2016 Jul-Aug;50(4):3-10

It was shown phenotypic changes in the root system of seedlings Arabidopsis thaliana in transgenic lines with overexpression and suppressed gene expression of serine-threonine protein kinase KIN10 in conditions of energy shortage and under normal conditions. The normal growth and development of KIN10 overexpressing plants with in energy deficiency conditions were detected. The significant inhibition of the plant development under normal conditions for these plant lines was obsereved. Read More

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December 2018
1 Read

[Ya.B. BLUME (on the occasion of the 60th anniversary of the birth)].

Authors:

Tsitol Genet 2016 Mar-Apr;50(2):83-4

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August 2016
8 Reads

[Elie Metchnikoff: father of phagocytosis theory and pioneer of experiments in vivo].

Tsitol Genet 2016 Mar-Apr;50(2):75-82

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August 2016
6 Reads

[THE ROLE OF (p)ppGpp MOLECULES IN FORMATION OF "STRICT RESPONSE" IN BACTERIA AND BIOSYNTHESIS OF ANTIBIOTICS AND MORPHOLOGICAL DIFFERENTIATION IN ACTINOMYCETES].

Tsitol Genet 2016 Mar-Apr;50(2):65-74

Strict response is a pleiotropic physiological response of cells caused by lack of aminoacetylated tRNAs. Experimentally, this response occurs due to the lack of amino acids in the environment and the limitation of tRNA aminoacylation even in the presence of the corresponding amino acids in the cell. Many features of this response indicate its dependence on the accumulation of ppGpp molecules. Read More

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August 2016
7 Reads

[CYTOSKELETON ORIENTATION IN THE EPIDERMAL CELLS OF ROOTS FORMED DE NOVO ON LEAF EXPLANTS UNDER CLINOROTATION].

Authors:
I V Bulavin

Tsitol Genet 2016 Mar-Apr;50(2):58-64

Root anatomy, cytoskeleton orientation and cell wall thickness in cells of the roots formed de novo in vitro under clinorotation (simulated microgravity) were investigated. Structure of the embryonic roots and of the roots formed de novo in cambium cells of the leaf petiole explants was shown to be similar. Root cell differentiation in vitro under clinorotation did not differ from that in control. Read More

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August 2016
9 Reads