Microorganisms 2022 Mar 4;10(3). Epub 2022 Mar 4.
Centre for Food-Borne and Animal Parasitology, Saskatoon Laboratory, Canadian Food Inspection Agency, 116 Veterinary Road, Saskatoon, SK S7N 2R3, Canada.
To facilitate the harmonized surveillance and investigation of cyclosporiasis outbreaks in the US and Canada, we adapted and verified the US-FDA's BAM 19b method and employed it in a national produce survey. Performance was verified by spiking 200, 10, 5 or 0 oocysts onto berries (50 ± 5 g, = 85) and 200, 10 or 0 oocysts onto green onions (25 ± 3 g, = 24) and leafy greens (25 ± 1 g, = 120) and testing these samples by the BAM method on Bio-Rad CFX96. Method robustness was assessed by aging (0 or 7 days) and freezing the produce and washes prior to testing, then implementing the method for the surveillance testing of 1759 imported leafy green, herb and berry samples. Read More