15,653 results match your criteria Current Stem Cell Research & Therapy[Journal]


Hypoxia Pathway Proteins in Normal and Malignant Hematopoiesis.

Cells 2019 Feb 13;8(2). Epub 2019 Feb 13.

Institute of Clinical Chemistry and Laboratory Medicine, Technische Universität Dresden, 01307 Dresden, Germany.

The regulation of oxygen (O₂) levels is crucial in embryogenesis and adult life, as O₂ controls a multitude of key cellular functions. Low oxygen levels (hypoxia) are relevant for tissue physiology as they are integral to adequate metabolism regulation and cell fate. Hence, the hypoxia response is of utmost importance for cell, organ and organism function and is dependent on the hypoxia-inducible factor (HIF) pathway. Read More

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http://dx.doi.org/10.3390/cells8020155DOI Listing
February 2019

Potential of Zerumbone as an Anti-Cancer Agent.

Molecules 2019 Feb 18;24(4). Epub 2019 Feb 18.

Cancer Biology Laboratory, DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB), Department of Biosciences & Bioengineering, Indian Institute of Technology, Guwahati, Assam 781039, India.

Cancer is still a major risk factor to public health globally, causing approximately 9.8 million deaths worldwide in 2018. Despite advances in conventional treatment modalities for cancer treatment, there are still few effective therapies available due to the lack of selectivity, adverse side effects, non-specific toxicities, and tumour recurrence. Read More

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http://dx.doi.org/10.3390/molecules24040734DOI Listing
February 2019

iPSC-derived MSCs are Functionally and Genetically Different from BM-MSCs.

Stem Cells 2019 Feb 19. Epub 2019 Feb 19.

The Regenerative Medicine Institute, National University of Ireland Galway, Galway, H91 CF50, Ireland.

There has been considerable interest in the generation of functional mesenchymal stromal cell (MSC) preparations from induced pluripotent stem cells (iMSCs) and this is now regarded as a potential source of unlimited, standardized, high-quality cells for therapeutic applications in regenerative medicine. While iMSCs meet minimal criteria for defining MSCs in terms of marker expression there are substantial differences in terms of trilineage potential, specifically a marked reduction in chondrogenic and adipogenic propensity in iMSCs compared to bone marrow-derived (BM) MSCs. To reveal the cellular basis underlying these differences we conducted phenotypic, functional and genetic comparisons between iMSCs and BM-MSCs. Read More

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http://dx.doi.org/10.1002/stem.2993DOI Listing
February 2019

Cervical cancer screening in rural India: Status & current concepts.

Indian J Med Res 2018 Dec;148(6):687-696

ICMR-National Institute of Cancer and Prevention Research, Noida, India.

Cervical carcinoma is one of the most common and dreaded diseases of women, and in India, it accounts for 16 per cent of total cervical cancer cases occurring globally. The situation is more alarming in the rural areas where the majority of women are illiterate and ignorant about the hazards of cervical cancer. Different screening strategies such as rural cancer registries and camp approach for cancer detection have been found useful in minimizing the problem of cervical cancer in the villages. Read More

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http://dx.doi.org/10.4103/ijmr.IJMR_5_17DOI Listing
December 2018

Statistical assessment and visualization of synergies for large-scale sparse drug combination datasets.

BMC Bioinformatics 2019 Feb 18;20(1):83. Epub 2019 Feb 18.

The Center of Cancer Research, Massachusetts General Hospital, 149 13th Street, Charlestown, MA, 02129, USA.

Background: Drug combinations have the potential to improve efficacy while limiting toxicity. To robustly identify synergistic combinations, high-throughput screens using full dose-response surface are desirable but require an impractical number of data points. Screening of a sparse number of doses per drug allows to screen large numbers of drug pairs, but complicates statistical assessment of synergy. Read More

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http://dx.doi.org/10.1186/s12859-019-2642-7DOI Listing
February 2019

Bone regeneration with umbilical cord blood mesenchymal stem cells in femoral defects of ovariectomized rats.

Osteoporos Sarcopenia 2018 Sep 26;4(3):95-101. Epub 2018 Sep 26.

Department of Anesthesiology and Pain Medicine, Chungnam National University Hospital, Daejeon, Korea.

Objectives: Current treatments for osteoporosis were prevention of progression, yet it has been questionable in the stimulation of bone growth. The mesenchymal stem cells (MSCs) treatment for osteoporosis aims to induce differentiation of bone progenitor cells into bone-forming osteoblasts. We investigate whether human umbilical cord blood (hUCB)-MSCs transplantation may induce bone regeneration for osteoporotic rat model induced by ovariectomy. Read More

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http://dx.doi.org/10.1016/j.afos.2018.08.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362973PMC
September 2018

Optical coherence tomography angiography for the anterior segment.

Eye Vis (Lond) 2019 1;6. Epub 2019 Feb 1.

1Singapore Eye Research Institute, Singapore National Eye Center, Singapore, Singapore.

Optical coherence tomography angiography (OCTA) is a rapid and non-invasive technique for imaging vasculature in the eye. As OCTA can produce high-resolution cross-sectional images and allow depth-resolved analysis for accurate localization of pathology of interest, it has become a promising method for anterior segment imaging. Furthermore, OCTA offers a more patient-friendly alternative to the conventional invasive dye-based fluorescent angiography. Read More

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http://dx.doi.org/10.1186/s40662-019-0129-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357412PMC
February 2019

Clinical Characteristics and Prognosis of Primary Tracheal Cancer: A Single Institution Experience.

Int J Hematol Oncol Stem Cell Res 2018 Oct;12(4):298-302

Department of Radiation Oncology, Cancer Care Manitoba, University of Manitoba, Winnipeg, Manitoba, Canada.

Primary tracheal cancers (PTCs) are rare and current evidence-based understanding is limited to retrospective reports and national databases. We present single institutional study of a historical cohort of PTC from Canadian provincial cancer registry database. After institutional research ethics board approval, all PTC patients diagnosed from 1980 to 2014 were identified through the Canadian provincial cancer registry. Read More

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October 2018
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Adrenal Collision Tumor Composed of Pheochromocytoma and Diffuse Large B-Cell Lymphoma: A Case Report.

Int J Hematol Oncol Stem Cell Res 2018 Oct;12(4):249-252

Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Adrenal involvement in the course of malignant lymphoma occurs in about 4% of patients, but primary adrenal lymphoma (PAL) is extremely rare. To the best of our knowledge, only one case study reported the combination of PAL with pheochromocytoma. In the current study, we present the second case who was a 63-year-old man admitted to our hospital with hematuria and abdominal discomfort. Read More

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October 2018

Metastatic tumor cells - genotypes and phenotypes.

Front Biol (Beijing) 2018 Aug 20;13(4):277-286. Epub 2018 Aug 20.

Department of Cardiothoracic Surgery, Department of Cell and Developmental Biology, Neuberger Berman Lung Cancer Center, Weill Cornell Medicine, New York, NY10065, USA.

Background: Metastasis is the primary cause of mortality in cancer patients. Therefore, elucidating the genetics and epigenetics of metastatic tumor cells and the mechanisms by which tumor cells acquire metastatic properties constitute significant challenges in cancer research.

Objective: To summarize the current understandings of the specific genotype and phenotype of the metastatic tumor cells. Read More

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http://dx.doi.org/10.1007/s11515-018-1513-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376987PMC

Incidence of infections after therapy completion in children with acute lymphoblastic leukemia or acute myeloid leukemia: a systematic review of the literature.

Leuk Lymphoma 2019 Feb 18:1-11. Epub 2019 Feb 18.

a Division of Haematology/Oncology, Department of Paediatrics , The Hospital for Sick Children , Toronto , Canada.

Infections are a common complication of treatment for pediatric acute lymphoblastic leukemia (ALL) or acute myeloid leukemia (AML). Less is known about infections occurring after treatment. We performed a systematic review of the literature to assess the incidence of infections after therapy completion in children and young adults with ALL or AML. Read More

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http://dx.doi.org/10.1080/10428194.2019.1573369DOI Listing
February 2019

Peripheral expression of long non-coding RNAs in bipolar patients.

J Affect Disord 2019 Feb 11;249:169-174. Epub 2019 Feb 11.

Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Background: Long non-coding RNAs (lncRNAs) have established roles in the pathogenesis of diverse human disorders including neuropsychiatric disorders.

Methods: In the current study, we evaluated expression levels of six apoptosis-related lncRNAs (CCAT2, TUG1, PANDA, NEAT1, FAS-AS1 and OIP5-AS1) in the peripheral blood of bipolar disorder (BD) patients and healthy subjects to assess their contribution in the pathogenesis of BD.

Results: CCAT2, TUG1 and PANDA were up-regulated in total BD patients compared with total healthy subjects (P values = 0. Read More

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http://dx.doi.org/10.1016/j.jad.2019.02.034DOI Listing
February 2019

Mitochondria-centric bioenergetic characteristics in cancer stem-like cells.

Arch Pharm Res 2019 Feb 15. Epub 2019 Feb 15.

Department of Surgery, Yonsei University Health System, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Korea.

Metabolic and genotoxic stresses that arise during tumor progression and anti-cancer treatment, respectively, can impose a selective pressure to promote cancer evolution in the tumor microenvironment. This process ultimately selects for the most "fit" clones, which generally have a cancer stem cell like phenotype with features of drug resistance, epithelial-mesenchymal transition, invasiveness, and high metastatic potential. From a bioenergetics perspective, these cancer stem-like cells (CSCs) exhibit mitochondria-centric energy metabolism and are capable of opportunistically utilizing available nutrients such as fatty acids to generate ATP and other metabolic substances, providing a selective advantage for their survival in an impermissible environment and metabolic context. Read More

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http://dx.doi.org/10.1007/s12272-019-01127-yDOI Listing
February 2019

The roles of signaling pathways in liver repair and regeneration.

J Cell Physiol 2019 Feb 15. Epub 2019 Feb 15.

Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

The liver has remarkable regeneration potency that restores liver mass and sustains body hemostasis. Liver regeneration through signaling pathways following resection or moderate damages are well studied. Various cell signaling, growth factors, cytokines, receptors, and cell types implicated in liver regeneration undergo controlled hypertrophy and proliferation. Read More

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http://dx.doi.org/10.1002/jcp.28336DOI Listing
February 2019

Current Therapeutic Results and Treatment Options for Older Patients with Relapsed Acute Myeloid Leukemia.

Cancers (Basel) 2019 Feb 14;11(2). Epub 2019 Feb 14.

Division of Hematology, Spedali Civili, 25123 Brescia, Italy.

Considerable progress has been made in the treatment of acute myeloid leukemia (AML). However, current therapeutic results are still unsatisfactory in untreated high-risk patients and poorer in those with primary refractory or relapsed disease. In older patients, reluctance by clinicians to treat unfit patients, higher AML cell resistance related to more frequent adverse karyotype and/or precedent myelodysplastic syndrome, and preferential involvement of chemorefractory early hemopoietic precursors in the pathogenesis of the disease further account for poor prognosis, with median survival lower than six months. Read More

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http://dx.doi.org/10.3390/cancers11020224DOI Listing
February 2019

Stem Cell-Derived Extracellular Vesicles for Treating Joint Injury and Osteoarthritis.

Nanomaterials (Basel) 2019 Feb 14;9(2). Epub 2019 Feb 14.

Raymond Purves Bone and Joint Research Laboratories, Institute of Bone and Joint Research, Kolling Institute, Northern Sydney Local Health District, Faculty of Medicine and Health, University of Sydney, St Leonards, NSW 2065, Australia.

Extracellular vesicles (EVs) are nanoscale particles secreted by almost all cell types to facilitate intercellular communication. Stem cell-derived EVs theoretically have the same biological functions as stem cells, but offer the advantages of small size, low immunogenicity, and removal of issues such as low cell survival and unpredictable long-term behaviour associated with direct cell transplantation. They have been an area of intense interest in regenerative medicine, due to the potential to harness their anti-inflammatory and pro-regenerative effects to induce healing in a wide variety of tissues. Read More

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http://dx.doi.org/10.3390/nano9020261DOI Listing
February 2019

Survival and functional outcomes of molecularly defined childhood posterior fossa ependymoma: Cure at a cost.

Cancer 2019 Feb 15. Epub 2019 Feb 15.

Division of Haematology/Oncology, Hospital for Sick Children, Toronto, Ontario, Canada.

Background: Posterior fossa ependymoma (PFE) comprises 2 groups, PF group A (PFA) and PF group B (PFB), with stark differences in outcome. However, to the authors' knowledge, the long-term outcomes of PFA ependymoma have not been described fully. The objective of the current study was to identify predictors of survival and neurocognitive outcome in a large consecutive cohort of subgrouped patients with PFE over 30 years. Read More

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http://dx.doi.org/10.1002/cncr.31995DOI Listing
February 2019

N-(3-methoxybenzyl)-(9Z,12Z,15Z)-octadecatrienamide promotes bone formation via the canonical Wnt/β-catenin signaling pathway.

Phytother Res 2019 Feb 15. Epub 2019 Feb 15.

Key Laboratory of Mental Health of the Ministry of Education, Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.

Osteoporosis is characterized by low bone mineral density and microarchitectural deterioration of bone tissue. N-(3-methoxybenzyl)-(9Z,12Z,15Z)-octadecatrienamide (MBOC) is one of the macamides isolated from Maca (Lepidium meyenii Walp.), a cruciferous plant from the Andes of Peru. Read More

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http://dx.doi.org/10.1002/ptr.6301DOI Listing
February 2019

Rap1 signal modulators control the maintenance of hematopoietic progenitors in bone marrow and adult long-term hematopoiesis.

Cancer Sci 2019 Feb 15. Epub 2019 Feb 15.

Medical Innovation Center, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto, 606-8507, Japan.

Adult long-term hematopoiesis depends on sustaining hematopoietic stem/progenitor cells (HSPCs) in bone marrow (BM) niches, where their balance of quiescence, self-renewal, and hematopoietic differentiation is tightly regulated. While various BM stroma cells that produce niche factors have been identified, regulation of the intrinsic responsiveness of HSPCs to the niche factors remains elusive. We previously reported that mice deficient for Sipa1, a Rap1 GTPase-activating protein, develop diverse hematopoietic disorders of late onset. Read More

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http://dx.doi.org/10.1111/cas.13974DOI Listing
February 2019

Immunomodulatory Functions of Mesenchymal Stem Cells in Tissue Engineering.

Stem Cells Int 2019 13;2019:9671206. Epub 2019 Jan 13.

Institute of Orthopedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma War Injuries, PLA, No. 28 Fuxing Road, Haidian District, Beijing 100853, China.

The inflammatory response to chronic injury affects tissue regeneration and has become an important factor influencing the prognosis of patients. In previous stem cell treatments, it was revealed that stem cells not only have the ability for direct differentiation or regeneration in chronic tissue damage but also have a regulatory effect on the immune microenvironment. Stem cells can regulate the immune microenvironment during tissue repair and provide a good "soil" for tissue regeneration. Read More

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http://dx.doi.org/10.1155/2019/9671206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350611PMC
January 2019

Targeting cholesterol metabolism in glioblastoma: a new therapeutic approach in cancer therapy.

J Investig Med 2019 Feb 14. Epub 2019 Feb 14.

Department of Human Anatomy and Cell Sciences, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.

Glioblastoma multiforme (GBM) is the most aggressive malignant brain tumor known with a poor survival rate despite current advances in the field of cancer. Additional research into the pathophysiology of GBM is urgently needed given the devastating nature of this disease. Recent studies have revealed the unique cellular physiology of GBM cells as compared with healthy astrocytes. Read More

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http://dx.doi.org/10.1136/jim-2018-000962DOI Listing
February 2019

Corrigendum to "Exosomes derived from TRAIL-engineered mesenchymal stem cells with effective anti-tumor activity in a mouse melanoma model" [International Journal of Pharmaceutics 549 (2018) 218-229].

Int J Pharm 2019 Mar;558:441

Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Exosomes are biological nano-sized vesicles (~30-200 nm in diameter) that are produced by a wide range of cells and play several roles in cell-cell communications. These vesicles contain membrane and cytoplasmic components of producing cells. Mesenchymal stem cells (MSCs) are the ideal producer of exosomes. Read More

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http://dx.doi.org/10.1016/j.ijpharm.2019.02.003DOI Listing
March 2019
2 Reads

Insulin-like growth factor-I promotes hepatic differentiation of human adipose tissue-derived stem cells.

Cell Biol Int 2019 Feb 13. Epub 2019 Feb 13.

Department of Clinical Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

There are numerous studies which provide support for the use of human adipose tissue-derived stem cells (hASCs) to generate hepatocyte-like cells. However, the produced cells exhibit only a certain level of differentiation, mainly due to inefficient induction conditions. Therefore, based on the important role of insulin-like growth factor (IGF-I) in hepatic function and development, in the current study we evaluated the differentiation efficacy of the mentioned factor to induce hASCs into functional hepatocyte-like cells. Read More

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http://dx.doi.org/10.1002/cbin.11113DOI Listing
February 2019

Can the Latest Computerized Technologies Revolutionize Conventional Assessment Tools and Therapies for a Neurological Disease? The Example of Parkinson's Disease.

Neurol Med Chir (Tokyo) 2019 Feb 13. Epub 2019 Feb 13.

Department of Neurosurgery, Hamamatsu University School of Medicine.

Dramatic breakthroughs in the treatment and assessment of neurological diseases are lacking. We believe that conventional methods have several limitations. Computerized technologies, including virtual reality, augmented reality, and robot assistant systems, are advancing at a rapid pace. Read More

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http://dx.doi.org/10.2176/nmc.ra.2018-0045DOI Listing
February 2019

Mesenchymal stem/stromal cell function in modulating cell death.

Stem Cell Res Ther 2019 Feb 13;10(1):56. Epub 2019 Feb 13.

Department of Environmental Medicine, Cooperative Medicine Unit, Research and Education Faculty, Medicine Science Cluster, Kochi Medical School (KMS), Kochi University, Kohasu, Oko-Cho, Nankoku City, Kochi Prefecture, 783-8505, Japan.

Mesenchymal stem/stromal cells (MSCs) delivered as cell therapy to individuals with degenerative and/or inflammatory disorders can help improve organ features and resolve inflammation, as demonstrated in preclinical studies and to some extent in clinical studies. MSCs have trophic, homing/migration, and immunosuppression functions, with many benefits in therapeutics. MSC functions are thought to depend on the paracrine action of soluble factors and/or the expression of membrane-bound molecules, mostly belonging to the molecular class of adhesion molecules, chemokines, enzymes, growth factors, and interleukins. Read More

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http://dx.doi.org/10.1186/s13287-019-1158-4DOI Listing
February 2019
1 Read

Endometrial Regeneration in Asherman's Syndrome: Clinical and Translational evidence of Stem Cell Therapies.

Curr Stem Cell Res Ther 2019 Feb 12. Epub 2019 Feb 12.

Department of Gynaecology and Obstetrics, Geneva University Hospital, 30, bvd de la Cluse, 1205, Genève. Switzerland.

Asherman's Syndrome or Intrauterine adhesions is an acquired uterine condition where fibrous scarring forms within the uterine cavity, resulting in reduced menstrual flow, pelvic pain and infertility. Until recently, the molecular mechanisms leading to the formation of fibrosis were poorly understood, and the treatment of Asherman's syndrome has largely focused on hysteroscopic resection of adhesions, hormonal therapy, and physical barriers. Numerous studies have begun exploring the molecular mechanisms behind the fibrotic process underlying Asherman's Syndrome as well as the role of stem cells in the regeneration of the endometrium as a treatment modality. Read More

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http://dx.doi.org/10.2174/1574888X14666190213100528DOI Listing
February 2019
1 Read

MIST1, an Inductive Signal for Salivary Amylase in Mesenchymal Stem Cells.

Int J Mol Sci 2019 Feb 12;20(3). Epub 2019 Feb 12.

Oral and Maxillofacial Diagnostic Sciences, University of Florida College of Dentistry, Gainesville, FL 32610, USA.

Sjögren's syndrome (SjS) is an autoimmune disease that destroys the salivary glands and results in severe dry mouth. Mesenchymal stem cell (MSC) transplantation has been recently proposed as a promising therapy for restoring cells in multiple degenerative diseases. We have recently utilized advanced proteomics biochemical assays to identify the key molecules involved in the mesenchymal-epithelial transition (MET) of co-cultured mouse bone-marrow-derived MSCs mMSCs with primary salivary gland cells. Read More

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http://dx.doi.org/10.3390/ijms20030767DOI Listing
February 2019
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Treatment of cancer stem cells from human colon adenocarcinoma cell line HT-29 with resveratrol and sulindac induced mesenchymal-endothelial transition rate.

Cell Tissue Res 2019 Feb 13. Epub 2019 Feb 13.

Faculty of Medicine, Department of Medical Biology, Ege University, Izmir, Turkey.

In the current experiment, the combined regime of resveratrol and a Wnt-3a inhibitor, sulindac, were examined on the angiogenic potential of cancer stem cells from human colon adenocarcinoma cell line HT-29 during 7 days. Cancer stem cells were enriched via a magnetic-activated cell sorter technique and cultured in endothelial induction medium containing sulindac and resveratrol. Expression of endothelial markers such as the von Willebrand factor (vWF) and vascular endothelial cadherin (VE-cadherin) and genes participating in mesenchymal-to-epithelial transition was studied by real-time PCR assay. Read More

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http://dx.doi.org/10.1007/s00441-019-02998-9DOI Listing
February 2019
1 Read

Intra-articular injection of mesenchymal stem cells for clinical outcomes and cartilage repair in osteoarthritis of the knee: a meta-analysis of randomized controlled trials.

Arch Orthop Trauma Surg 2019 Feb 11. Epub 2019 Feb 11.

Department of Orthopedic Surgery, Chung-Ang University Hospital, Chung-Ang University College of Medicine, 102 Heukseok-ro, Dongjak-gu, 06973, Seoul, South Korea.

Introduction: Mesenchymal stem cells (MSCs) have gained popularity for articular cartilage repair. However, efficacy of intra-articular MSCs in osteoarthritis remains unclear. In the setting of a meta-analysis of randomized controlled trials (RCTs), we aimed to investigate the efficacy of intra-articular MSCs on clinical outcomes and cartilage repair in patients with knee osteoarthritis. Read More

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http://dx.doi.org/10.1007/s00402-019-03140-8DOI Listing
February 2019

ER stress sensor, glucose regulatory protein 78 (GRP78) regulates redox status in pancreatic cancer thereby maintaining "stemness".

Cell Death Dis 2019 Feb 12;10(2):132. Epub 2019 Feb 12.

Department of Surgery, University of Miami, Miami, FL, USA.

Endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) signaling have been shown to be dysregulated in multiple cancer types. Glucose regulatory protein 78 (GRP78), the master regulator of the UPR, plays a role in proliferation, invasion, and metastasis in cancer. Cancer stem cells (CSCs) make up a crucial component of the tumor heterogeneity in pancreatic cancer, as well as other cancers. Read More

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http://www.nature.com/articles/s41419-019-1408-5
Publisher Site
http://dx.doi.org/10.1038/s41419-019-1408-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372649PMC
February 2019
3 Reads

5-O-Acetyl-Renieramycin T from Blue Sponge Xestospongia sp. Induces Lung Cancer Stem Cell Apoptosis.

Mar Drugs 2019 Feb 11;17(2). Epub 2019 Feb 11.

Cell-based Drug and Health Products Development Research Unit, Chulalongkorn University, Bangkok 10330, Thailand.

Lung cancer is one of the most significant cancers as it accounts for almost 1 in 5 cancer deaths worldwide, with an increasing incident rate. Management of the cancer has been shown to frequently fail due to the ability of the cancer cells to resist therapy as well as metastasis. Recent evidence has suggested that the poor response to the current treatment drugs and the ability to undergo metastasis are driven by cancer stem cells (CSCs) within the tumor. Read More

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http://dx.doi.org/10.3390/md17020109DOI Listing
February 2019
1 Read

Radiation induced DNA damage response and resistance in colorectal cancer stem-like cells.

Int J Radiat Biol 2019 Feb 12:1-23. Epub 2019 Feb 12.

a Molecular Stress and Stem Cell Biology Group , School of Biotechnology, KIIT University , Bhubaneswar , Odisha - 751024 , India.

Purpose: Radiation therapy is an integral part of current treatment modality for colorectal cancer. Recent studies have revealed the presence of cancer stem-like cells (CSCs) population, in different tumors are responsible for therapeutic resistance and disease relapse, including colorectal cancer with poorer survival rate. Hence, characterization of effect of Ionizing Radiation (IR) in colorectal cancer may serve to explain possible mechanisms. Read More

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http://dx.doi.org/10.1080/09553002.2019.1580401DOI Listing
February 2019

Improved chondrogenic response of mesenchymal stem cells to a polyethersulfone/polyaniline blended nanofibrous scaffold.

J Cell Biochem 2019 Feb 11. Epub 2019 Feb 11.

Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran.

Owing to the fact that the cartilage tissue is not able to repair itself, the treatment of the joint damages is very difficult by current methods. Induction of tissue repair requires suitable cell and extracellular matrix. Providing these two parts can only be done using tissue engineering. Read More

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http://dx.doi.org/10.1002/jcb.28412DOI Listing
February 2019

Increased splenic human CD4:CD8 T cell ratios, serum human interferon-γ and intestinal human interleukin-17 are associated with clinical graft-versus-host disease in humanized mice.

Transpl Immunol 2019 Feb 8. Epub 2019 Feb 8.

School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2252, Australia; Molecular Horizons, University of Wollongong, Wollongong, NSW 2252, Australia; Illawarra Health and Medical Research Institute, Wollongong, NSW 2252, Australia. Electronic address:

Graft-versus-host disease (GVHD) is a frequent complication following allogeneic hematopoietic stem cell transplantation (HSCT) with current therapies limited to general immunosuppression. Humanized mouse models of GVHD are emerging as valuable intermediaries to allow translation of findings from allogeneic mouse models to humans to prevent and treat this disease, but such models require further characterization. In this study, humanized mice were generated by injecting immunodeficient non-obese diabetic severe combined immunodeficiency interleukin (IL)-2 receptor γ common chain null (NSG) mice with human peripheral blood mononuclear cells (hPBMCs). Read More

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http://dx.doi.org/10.1016/j.trim.2019.02.003DOI Listing
February 2019
1 Read

Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro.

Eur Cell Mater 2019 02 11;37:88-112. Epub 2019 Feb 11.

IZKF Group Tissue Regeneration in Musculoskeletal Diseases, Orthopedic Center for Musculoskeletal Research, Würzburg,

Mesenchymal stem/stromal cells (MSCs) are an essential element of most modern tissue engineering and regenerative medicine approaches due to their multipotency and immunoregulatory functions. Despite the prospective value of MSCs for the clinics, the stem cells community is questioning their developmental origin, in vivo localization, identification, and regenerative potential after several years of far-reaching research in the field. Although several major progresses have been made in mimicking the complexity of the MSC niche in vitro, there is need for comprehensive studies of fundamental mechanisms triggered by microenvironmental cues before moving to regenerative medicine cell therapy applications. Read More

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http://dx.doi.org/10.22203/eCM.v037a07DOI Listing
February 2019
1 Read

Characterization of CD133/CD44 human prostate cancer stem cells with ATR-FTIR spectroscopy.

Analyst 2019 Feb 11. Epub 2019 Feb 11.

Department of Stem Cell, Ege University Health Science Institute, Izmir, 35100, Turkey. and Department of Embryology and Histology, School of Medicine, Ege University, 35100, Izmir, Turkey.

Current cancer treatments destroy the tumor mass but cannot prevent the recurrence of cancer. The heterogeneous structure of the tumor mass includes cancer stem cells that are responsible for tumor relapse, treatment resistance, invasion and metastasis. The biology of these cells is still not fully understood; therefore, effective treatments cannot be developed sufficiently. Read More

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http://dx.doi.org/10.1039/c9an00093cDOI Listing
February 2019

Functional expression of the mechanosensitive PIEZO1 channel in primary endometrial epithelial cells and endometrial organoids.

Sci Rep 2019 Feb 11;9(1):1779. Epub 2019 Feb 11.

Laboratory of Endometrium, Endometriosis & Reproductive Medicine, Department of Development and Regeneration, KU Leuven, Herestraat 49 box 611, 3000, Leuven, Belgium.

Successful pregnancy requires the establishment of a complex dialogue between the implanting embryo and the endometrium. Knowledge regarding molecular candidates involved in this early communication process is inadequate due to limited access to primary human endometrial epithelial cells (EEC). Since pseudo-pregnancy in rodents can be induced by mechanical scratching of an appropriately primed uterus, this study aimed to investigate the expression of mechanosensitive ion channels in EEC. Read More

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http://dx.doi.org/10.1038/s41598-018-38376-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370865PMC
February 2019
1 Read

Current approaches in identification and isolation of cancer stem cells.

J Cell Physiol 2019 Feb 11. Epub 2019 Feb 11.

Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Cancer stem cells (CSCs) are tumor cells with initiating ability, self-renewal potential, and intrinsic resistance to conventional therapeutics. Efficient isolation and characterization of CSCs pave the way for more comprehensive knowledge about tumorigenesis, heterogeneity, and chemoresistance. Also a better understanding of CSCs will lead to novel era of both basic and clinical cancer research, reclassification of human tumors, and development of innovative therapeutic strategies. Read More

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http://doi.wiley.com/10.1002/jcp.28271
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http://dx.doi.org/10.1002/jcp.28271DOI Listing
February 2019
4 Reads

Prognostic Significance of Complex Karyotypes in Acute Myeloid Leukemia.

Curr Treat Options Oncol 2019 Feb 11;20(2):15. Epub 2019 Feb 11.

Department of Pathology, University of Chicago, Chicago, IL, USA.

Opinion Statement: Acute myeloid leukemia (AML) patients with a complex karyotype (CK-AML) show at least 3 unrelated clonal cytogenetic abnormalities with notoriously poor outcome. Such cases fall into either AML with myelodysplasia-related changes or therapy-related AML in the current World Health Organization classification of AML. Allogeneic stem cell transplantation is one of the only treatment modalities that can provide a long-term survival benefit and is recommended as a consolidative treatment in patients who are able to achieve complete remission. Read More

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http://dx.doi.org/10.1007/s11864-019-0612-yDOI Listing
February 2019
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Biology and therapy of primary mediastinal B-cell lymphoma: current status and future directions.

Br J Haematol 2019 Feb 10. Epub 2019 Feb 10.

Blood Cancer Research Group, Mater Research, University of Queensland, Translational Research Institute, Brisbane, QLD, Australia.

Primary mediastinal B-cell lymphoma (PMBCL) is a distinct disease closely related to classical nodular sclerosing Hodgkin lymphoma. Conventional diagnostic paradigms utilising clinical, morphological and immunophenotypical features can be challenging due to overlapping features with other B-cell lymphomas. Reliable diagnostic and prognostic biomarkers that are applicable to the conventional diagnostic laboratory are largely lacking. Read More

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http://dx.doi.org/10.1111/bjh.15778DOI Listing
February 2019
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Enteroids: Promising in Vitro Models for Studies of Intestinal Physiology and Nutrition in Farm Animals.

J Agric Food Chem 2019 Feb 19. Epub 2019 Feb 19.

Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture Chinese Academy of Sciences, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture , Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process , Changsha , Hunan 410125 , China.

The lack of sophisticated in vitro models limits our current understanding of gastrointestinal functions in farm animals. Conventional 2D cell lines or primary cells fail to recapitulate the physiology of in vivo intestinal epithelium. In contrast stem cell-derived, nontransformed 3D enteroids partially recreate the villus-crypt anatomy of the native intestine and comprise most if not all intestinal cell types including enterocytes, enteroendocrine cells, goblet cells, Paneth cells, and stem cells. Read More

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http://dx.doi.org/10.1021/acs.jafc.8b06908DOI Listing
February 2019
2.912 Impact Factor

An improved clonogenic culture method for thymic epithelial cells.

J Immunol Methods 2019 Feb 6. Epub 2019 Feb 6.

Department of Immunology and Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan; Center for iPS Cell Research and Application (CiRA), Laboratory of Immunobiology, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. Electronic address:

A clonogenic assay system for thymic epithelial cells (TECs) is of crucial importance for identifying thymic epithelial stem and/or progenitor cells, evaluating their activities, and understanding the mechanisms of thymic involution. However, current systems are not sufficiently sensitive at detecting and quantifying TEC colonies from the adult thymus. Here, we optimized the culture condition to detect visible colonies from adult TECs by modifying our previous culture methods. Read More

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http://dx.doi.org/10.1016/j.jim.2019.02.003DOI Listing
February 2019
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Predicting Polygenic Risk of Psychiatric Disorders.

Biol Psychiatry 2018 Dec 28. Epub 2018 Dec 28.

Analytic and Translational Genetics Unit, Massachusetts General Hospital, Boston, Massachusetts; Program in Medical and Population Genetics, Broad Institute of Harvard and MIT, Cambridge, Massachusetts; Stanley Center for Psychiatric Research, Broad Institute of Harvard and MIT, Cambridge, Massachusetts.

Genetics provides two major opportunities for understanding human disease-as a transformative line of etiological inquiry and as a biomarker for heritable diseases. In psychiatry, biomarkers are very much needed for both research and treatment, given the heterogenous populations identified by current phenomenologically based diagnostic systems. To date, however, useful and valid biomarkers have been scant owing to the inaccessibility and complexity of human brain tissue and consequent lack of insight into disease mechanisms. Read More

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http://dx.doi.org/10.1016/j.biopsych.2018.12.015DOI Listing
December 2018

An ultrasensitive test for profiling circulating tumor DNA using integrated comprehensive droplet digital detection.

Lab Chip 2019 Feb 8. Epub 2019 Feb 8.

Sue and Bill Gross Stem Cell Research Center, Sue & Bill Gross Hall CIRM Institute, University of California, Irvine, 845 Health Sciences Road, Suite 3027, Irvine, CA 92697, USA. and Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA 92697, USA and Chao Family Comprehensive Cancer Center, University of California, Irvine, Irvine, CA 92697, USA and Edwards Life Sciences Center for Advanced Cardiovascular Technology, University of California, Irvine, Irvine, CA 92697, USA and Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA and Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA.

Current cancer detection systems lack the required sensitivity to reliably detect minimal residual disease (MRD) and recurrence at the earliest stages when treatment would be most effective. To address this issue, we present a novel liquid biopsy approach that utilizes an integrated comprehensive droplet digital detection (IC3D) digital PCR system which combines microfluidic droplet partitioning, fluorescent multiplex PCR chemistry, and our rapid 3D, large-volume droplet counting technology. The IC3D ddPCR assay can detect cancer-specific, ultra-rare genomic targets due to large sample input and high degree of partitioning. Read More

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http://dx.doi.org/10.1039/c8lc01399cDOI Listing
February 2019
2 Reads

as a Genetic Model for Hematopoiesis.

Genetics 2019 02;211(2):367-417

Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095

In this FlyBook chapter, we present a survey of the current literature on the development of the hematopoietic system in The blood system consists entirely of cells that function in innate immunity, tissue integrity, wound healing, and various forms of stress response, and are therefore functionally similar to myeloid cells in mammals. The primary cell types are specialized for phagocytic, melanization, and encapsulation functions. As in mammalian systems, multiple sites of hematopoiesis are evident in and the mechanisms involved in this process employ many of the same molecular strategies that exemplify blood development in humans. Read More

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http://dx.doi.org/10.1534/genetics.118.300223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366919PMC
February 2019
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Desferrioxamine-caffeine shows improved efficacy in chelating iron and depleting cancer stem cells.

J Trace Elem Med Biol 2019 Mar 9;52:232-238. Epub 2019 Jan 9.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Iron chelation has already been proposed to be a feasible strategy for cancer therapeutics in that reinforced iron demand is demonstrated in cancer cells, and quite a few iron chelators have been developed for this purpose. Desferrioxamine (DFO), an iron chelator approved by the U.S. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0946672X183057
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http://dx.doi.org/10.1016/j.jtemb.2019.01.004DOI Listing
March 2019
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Adaptive NK cell responses in HIV/SIV infections: A roadmap to cell-based therapeutics?

J Leukoc Biol 2019 Feb 7. Epub 2019 Feb 7.

Center for Virology and Vaccine Research (CVVR), Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.

NK cells play a critical role in antiviral and antitumor responses. Although current NK cell immune therapies have focused primarily on cancer biology, many of these advances can be readily applied to target HIV/simian immunodeficiency virus (SIV)-infected cells. Promising developments include recent reports that CAR NK cells are capable of targeted responses while producing less off-target and toxic side effects than are associated with CAR T cell therapies. Read More

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http://dx.doi.org/10.1002/JLB.MR0718-303RDOI Listing
February 2019

CAR T cell immunotherapy for Multiple Myeloma. A review of current data and potential clinical applications.

Am J Hematol 2019 Feb 7. Epub 2019 Feb 7.

Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA.

Multiple myeloma (MM) is a malignant plasma cell disorder that remains incurable for most patients despite significant improvements achieved with modern therapy. Tumor evasion is a key process in the pathogenesis of MM and a compromised immune system is associated with more aggressive forms of the disease. In contrast, the emergence of myeloma-specific immune responses after both autologous and allogeneic stem cell transplantation is associated with better prognosis. Read More

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http://dx.doi.org/10.1002/ajh.25428DOI Listing
February 2019

Exploring the roles of MSCs in infections: focus on bacterial diseases.

J Mol Med (Berl) 2019 Feb 7. Epub 2019 Feb 7.

Department of Experimental, Diagnostic and Specialty Medicine, Unit of Histology, Embryology and Applied Biology, University of Bologna, Via Belmeloro 8, 40126, Bologna, Italy.

Despite human healthcare advances, some microorganisms continuously react evolving new survival strategies, choosing between a commensal fitness and a pathogenic attitude. Many opportunistic microbes are becoming an increasing cause of clinically evident infections while several renowned infectious diseases sustain a considerable number of deaths. Besides the primary and extensively investigated role of immune cells, other cell types are involved in the microbe-host interaction during infection. Read More

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http://link.springer.com/10.1007/s00109-019-01752-6
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http://dx.doi.org/10.1007/s00109-019-01752-6DOI Listing
February 2019
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Selective hematopoietic stem cell ablation using CD117-antibody-drug-conjugates enables safe and effective transplantation with immunity preservation.

Nat Commun 2019 Feb 6;10(1):617. Epub 2019 Feb 6.

Program in Cellular and Molecular Medicine, Department of Medicine, Boston Children's Hospital, Boston, MA, 02115, USA.

Hematopoietic stem cell transplantation (HSCT) is a curative therapy for blood and immune diseases with potential for many settings beyond current standard-of-care. Broad HSCT application is currently precluded largely due to morbidity and mortality associated with genotoxic irradiation or chemotherapy conditioning. Here we show that a single dose of a CD117-antibody-drug-conjugate (CD117-ADC) to saporin leads to > 99% depletion of host HSCs, enabling rapid and efficient donor hematopoietic cell engraftment. Read More

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http://dx.doi.org/10.1038/s41467-018-08201-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365495PMC
February 2019
1 Read