16,011 results match your criteria Current Stem Cell Research & Therapy[Journal]


A Microparticle Approach for Non-viral Gene Delivery within 3D Human Mesenchymal Stromal Cell Aggregates.

Acta Biomater 2019 Apr 17. Epub 2019 Apr 17.

Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA; Materials Science Program, University of Wisconsin, Madison, WI 53706, USA; Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI 53705, USA; AO Foundation Collaborative Research Center, Davos, Switzerland. Electronic address:

Three-dimensional (3D) multicellular aggregates, in comparison to two-dimensional monolayer culture, can provide tissue culture models that better recapitulate the abundant cell-cell and cell-matrix interactions found in vivo. In addition, aggregates are potentially useful building blocks for tissue engineering. However, control over the interior aggregate microenvironment is challenging due to inherent barriers for diffusion of biological mediators (e. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.04.038DOI Listing

Primary cilium and brain aging: role in neural stem cells, neurodegenerative diseases and glioblastoma.

Ageing Res Rev 2019 Apr 17. Epub 2019 Apr 17.

Cellular Oncology group, Biodonostia Health Research Institute, San Sebastian, Spain; CIBER de Fragilidad y Envejecimiento Saludable (CIBERfes), Madrid, Spain; IKERBASQUE, Basque Foundation, Bilbao, Spain. Electronic address:

Brain aging is characterized by a progressive loss of tissue integrity and function as a consequence of impaired homeostasis and regeneration capacities. The primary cilium is a highly conserved organelle that projects from the cell surface in a single copy in virtually all mammalian cell types including neural stem/progenitors cells and neurons. Increasing evidence in the last decade points out that primary cilium could be a relevant mediator of neural stem cell activity, neurogenesis, neuronal maturation and maintenance, and brain tumorigenesis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15681637183030
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http://dx.doi.org/10.1016/j.arr.2019.04.004DOI Listing
April 2019
1 Read

Evaluating laboratory criteria for combined immunodeficiency in adult patients diagnosed with common variable immunodeficiency.

Clin Immunol 2019 Apr 17. Epub 2019 Apr 17.

Department for Rheumatology and Clinical Immunology and Center for Chronic Immunodeficiency (CCI), Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Germany; Center for Chronic Immunodeficiency (CCI), Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Germany. Electronic address:

Some patients diagnosed with common variable immunodeficiency (CVID) actually suffer from combined immunodeficiency (CID) and therefore may require a different, CID-adapted treatment. Several CD4 T-cell-based criteria have been proposed in the past to identify patients with CID within the cohort of adult CVID patients. In this monocentric study, we used retrospective immunological and clinical data of 238 CVID patients to compare four different proposals of how to define CID among CVID patients. Read More

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http://dx.doi.org/10.1016/j.clim.2019.04.001DOI Listing

The patient-independent human iPSC model - a new tool for rapid determination of genetic variant pathogenicity in long QT syndrome.

Heart Rhythm 2019 Apr 17. Epub 2019 Apr 17.

(1)Vanderbilt University School of Medicine, Nashville, TN, USA; (2)Vanderbilt Center for Arrhythmia Research and Therapeutics, Department of Medicine, Nashville, TN, USA. Electronic address:

Background: Commercial genetic testing for Long QT Syndrome (LQTS) has rapidly expanded, but the inability to accurately predict whether a rare variant is pathogenic has limited its clinical benefit. Novel missense variants are routinely reported as "Variant of Unknown Significance (VUS)" and cannot be used to screen family members at-risk for sudden cardiac death. Better approaches to determine pathogenicity of VUS are needed. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15475271193036
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http://dx.doi.org/10.1016/j.hrthm.2019.04.031DOI Listing
April 2019
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GAS8 and its naturally occurring antisense RNA as biomarkers in multiple sclerosis.

Immunobiology 2019 Apr 13. Epub 2019 Apr 13.

Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Expressions of the Growth arrest specific 8 (GAS8) and its naturally occurring anti-sense RNA (GAS8-AS1) have been assessed in tumoral tissues of different origins. However, their association with immune-related disorders has been poorly understood. In the current study, we evaluated expression levels of these genes in 50 relapsing-remitting multiple sclerosis (RRMS) patients compared with age- and sex-matched controls. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01712985193004
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http://dx.doi.org/10.1016/j.imbio.2019.04.005DOI Listing
April 2019
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Honokiol for cancer therapeutics: A traditional medicine that can modulate multiple oncogenic targets.

Pharmacol Res 2019 Apr 16. Epub 2019 Apr 16.

Cancer Biology Laboratory and DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB) Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, 781039, India. Electronic address:

In spite of billions of dollars expended on cancer research every year, the incidence rate and the mortality rate due to this widespread disease has increased drastically over the last few decades. Recent reports from the World Health Organization advocate that overall global cancer burden and deaths due to cancer are expected to double by the next decade. Synthetic drugs developed as chemotherapeutics have repeatedly shown adverse side effects and development of chemoresistance. Read More

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http://dx.doi.org/10.1016/j.phrs.2019.04.004DOI Listing
April 2019
2 Reads

Nicotinamide mononucleotide promotes osteogenesis and reduces adipogenesis by regulating mesenchymal stromal cells via the SIRT1 pathway in aged bone marrow.

Cell Death Dis 2019 Apr 18;10(5):336. Epub 2019 Apr 18.

Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

Mesenchymal stromal cells (MSCs) can differentiate to various cell types including osteoblasts, chondrocytes, and adipocytes. This cellular flexibility contributes to widespread clinical use of MSCs in tissue repair. However, challenges remain in efficient cellular expansion of MSCs for stem cell therapy. Read More

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http://dx.doi.org/10.1038/s41419-019-1569-2DOI Listing
April 2019
2 Reads

Differentiating Human Induced Pluripotent Stem Cells (iPSCs) Into Lung Epithelial Cells.

Curr Protoc Stem Cell Biol 2019 Apr 18:e86. Epub 2019 Apr 18.

Eyestem Research, Centre for Cellular and Molecular Platforms (CCAMP), National Centre for Biological Sciences (NCBS) Campus, Bengaluru, India.

Human induced pluripotent stem cells (hiPSCs) not only offer great opportunities for the study of human development but also have tremendous potential for future clinical cell-based therapies. The protocol outlined here is used to differentiate hiPSCs into lung epithelial cell types through a process that faithfully recapitulates the stepwise events observed in vivo. From pluripotency, cells are differentiated to a definitive endoderm fate, followed by progression into anteriorized foregut endoderm that has the ability to give rise to both proximal and distal epithelial cells. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/cpsc.86
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http://dx.doi.org/10.1002/cpsc.86DOI Listing
April 2019
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Bone marrow donor selection and characterization of MSCs is critical for pre-clinical and clinical cell dose production.

J Transl Med 2019 Apr 17;17(1):128. Epub 2019 Apr 17.

Department of Laboratory Medicine, University of California, San Francisco, 513 Parnassus Avenue, HSE 760, San Francisco, CA, 94143, USA.

Background: Cell based therapies, such as bone marrow derived mesenchymal stem cells (BM-MSCs; also known as mesenchymal stromal cells), are currently under investigation for a number of disease applications. The current challenge facing the field is maintaining the consistency and quality of cells especially for cell dose production for pre-clinical testing and clinical trials. Here we determine how BM-donor variability and thus the derived MSCs factor into selection of the optimal primary cell lineage for cell production and testing in a pre-clinical swine model of trauma induced acute respiratory distress syndrome. Read More

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http://dx.doi.org/10.1186/s12967-019-1877-4DOI Listing
April 2019
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Comprehensive analysis of isolated der(1;7)(q10;p10) in a large international homogenous cohort of patients with myelodysplastic syndromes.

Genes Chromosomes Cancer 2019 Apr 17. Epub 2019 Apr 17.

Clinics of Hematology and Medical Oncology, University Medical Center Göttingen, Göttingen, Germany.

The karyotype is a strong independent prognostic factor in myelodysplastic syndromes (MDS). Since the implementation of the new comprehensive cytogenetic scoring system for MDS, chromosome 7 anomalies are no longer generally assigned to poor risk features but are thoroughly separated. However, der(1;7)(q10;p10), hereinafter der(1;7), is merged into the group labelled "any other single" and belongs to the intermediate risk group, just by definition due to lack of adequate clinical data. Read More

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http://dx.doi.org/10.1002/gcc.22760DOI Listing

Integrating genetic mutations and expression profiles for survival prediction of lung adenocarcinoma.

Thorac Cancer 2019 Apr 16. Epub 2019 Apr 16.

Stem Cell Translational Research Center, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

Background: Lung adenocarcinoma (LUAD) is a set of heterogeneous diseases with distinct genetic and transcriptomic characteristics. Since the introduction of the 2011 International Association for the Study of Lung Cancer/American Thoracic Society/European Respiratory Society histologic classification, increasing evidence has provided insights into genomic mutations and rearrangements among individual histologic subtypes of LUAD. However, how genotypic and phenotypic features of LUAD are interconnected is not well understood. Read More

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http://dx.doi.org/10.1111/1759-7714.13072DOI Listing

NK cells: An attractive candidate for cancer therapy.

J Cell Physiol 2019 Apr 16. Epub 2019 Apr 16.

Stem Cell Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran.

Natural killer (NK) cells have significant capability in tumor immune-surveillance. The ability of lyse transformed cells immediately in an antigen-independent manner make them an attractive candidate for cancer cell therapy. Despite employment of NK cells in cancer immunotherapy, clinical trials are faced with serious limitations such as trouble with the penetration of NK cells in tumor sites, limited in vivo persistence, and tumor microenvironment interference. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.28657
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http://dx.doi.org/10.1002/jcp.28657DOI Listing
April 2019
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CXCR7 signaling promotes breast cancer survival in response to mesenchymal stromal stem cell-derived factors.

Cell Death Discov 2019 11;5:87. Epub 2019 Apr 11.

3Cancer Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), PO Box 34110, Doha, Qatar.

The interaction between cancer cells and molecular cues provided by tumor stromal cells plays a crucial role in cancer growth and progression. We have recently reported that the outcome of interaction between tumor cells and stromal cells is dependent on the gene expression signature of tumor cells. In the current study, we observed that several cancer cell lines, e. Read More

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http://dx.doi.org/10.1038/s41420-019-0169-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459874PMC
April 2019
1 Read

Transcriptomic analysis reveals differences in the regulation of amino acid metabolism in asexual and sexual planarians.

Sci Rep 2019 Apr 16;9(1):6132. Epub 2019 Apr 16.

Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori, 036-8561, Japan.

Many flatworms can alternate between asexual and sexual reproduction. This is a powerful reproductive strategy enabling them to benefit from the features of the two reproductive modes, namely, rapid multiplication and genetic shuffling. The two reproductive modes are enabled by the presence of pluripotent adult stem cells (neoblasts), by generating any type of tissue in the asexual mode, and producing and maintaining germ cells in the sexual mode. Read More

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http://dx.doi.org/10.1038/s41598-019-42025-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6467871PMC

Preclinical insights into cholangiopathies: disease modeling and emerging therapeutic targets.

Expert Opin Ther Targets 2019 Apr 16. Epub 2019 Apr 16.

a Indiana Center for Liver Research, Division of Gastroenterology & Hepatology, Department of Medicine , Indiana University School of Medicine , Indianapolis , Indiana , USA.

Introduction The common predominant clinical features of cholangiopathies such as primary sclerosing cholangitis (PSC), primary biliary cholangitis (PBC), and biliary atresia (BA) are biliary damage/senescence and liver fibrosis. Curative therapies are lacking, and liver transplantation is only option. An understanding of the mechanisms and pathogenesis is needed to develop novel therapies. Read More

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http://dx.doi.org/10.1080/14728222.2019.1608950DOI Listing
April 2019
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Outcome of Philadelphia Positive Acute Lymphoblastic Leukemia With or Without Allogeneic Stem Cell Transplantation in a Retrospective Study.

Indian J Hematol Blood Transfus 2019 Apr 1;35(2):240-247. Epub 2018 Sep 1.

Department of Hemato-Oncology, Rajiv Gandhi Cancer Institute and Research Centre, Sector-5, Rohini, New Delhi, 110085 India.

Philadelphia positive ALL (Ph + ALL) is an aggressive leukemia associated with lower remission rates and poor survival. Current treatment approach for Ph + ALL is chemotherapy along with TKI and CNS directed therapy followed by Allogeneic stem cell transplantation (Allo-SCT). To analyze outcome of Ph + ALL with or without Allo-SCT in the era of universal TKI uses. Read More

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http://link.springer.com/10.1007/s12288-018-1005-2
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http://dx.doi.org/10.1007/s12288-018-1005-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439021PMC
April 2019
2 Reads

Therapeutic challenges and current immunomodulatory strategies in targeting the immunosuppressive pancreatic tumor microenvironment.

J Exp Clin Cancer Res 2019 Apr 15;38(1):162. Epub 2019 Apr 15.

School of Pharmacy, International Medical University, Kuala Lumpur, Malaysia.

Background: Pancreatic cancer is one of the most lethal type of cancers, with an overall five-year survival rate of less than 5%. It is usually diagnosed at an advanced stage with limited therapeutic options. To date, no effective treatment options have demonstrated long-term benefits in advanced pancreatic cancer patients. Read More

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https://jeccr.biomedcentral.com/articles/10.1186/s13046-019-
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http://dx.doi.org/10.1186/s13046-019-1153-8DOI Listing
April 2019
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Targeting Hedgehog (Hh) Pathway for the Acute Myeloid Leukemia Treatment.

Cells 2019 Apr 3;8(4). Epub 2019 Apr 3.

Department of Hematology, National Cancer Center Hospital East, Kashiwa 277-8577, Japan.

The Hedgehog (Hh) pathway, containing the Patched (PTCH) and Smoothened (SMO) multitransmembrane proteins, is the main regulator of vertebrate embryonic development. A non-canonical Hh pathway was recently observed in numerous types of solid cancers and hematological malignancies. Although acute myeloid leukemia (AML) is a common and lethal myeloid malignancy, the chemotherapy for AML has not changed in the last three decades. Read More

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http://dx.doi.org/10.3390/cells8040312DOI Listing
April 2019
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Current Status and Perspectives of Human Mesenchymal Stem Cell Therapy.

Stem Cells Int 2019 7;2019:4762634. Epub 2019 Mar 7.

Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.

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http://dx.doi.org/10.1155/2019/4762634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431493PMC

The Spectrum of Scarring in Craniofacial Wound Repair.

Front Physiol 2019 29;10:322. Epub 2019 Mar 29.

Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Stanford University School of Medicine, Stanford, CA, United States.

Fibrosis is intimately linked to wound healing and is one of the largest causes of wound-related morbidity. While scar formation is the normal and inevitable outcome of adult mammalian cutaneous wound healing, scarring varies widely between different anatomical sites. The spectrum of craniofacial wound healing spans a particularly diverse range of outcomes. Read More

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http://dx.doi.org/10.3389/fphys.2019.00322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450464PMC

Human Umbilical Cord-derived Scaffolds for Cartilage Tissue Engineering.

J Biomed Mater Res A 2019 Apr 15. Epub 2019 Apr 15.

Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, 1665659911, ACECR, Tehran, Iran.

Since articular cartilage is an avascular tissue, it has limited self-regeneration capacity after damage. Current methods for human bone marrow-derived Mesenchymal Stem Cell (hBM-MSC) differentiation into cartilage result in tissues with a lower quality as compared to native articular cartilage. Decellularized biological scaffolds have the potential to provide appropriate signals, in order to support cellular retention, migration, proliferation and differentiation. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.a.36698
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http://dx.doi.org/10.1002/jbm.a.36698DOI Listing
April 2019
1 Read

Tumor necrosis factor alpha and interleukin-5 inhibit olfactory regeneration via apoptosis of olfactory sphere cells in mice models of allergic rhinitis.

Clin Exp Allergy 2019 Apr 13. Epub 2019 Apr 13.

Division of Pediatric Neurosurgery, Seoul National University Children's Hospital, Seoul National University College of Medicine, Seoul, South Korea.

Background: Olfactory dysfunction is frequently experienced by patients with allergic rhinitis. It is thought to result from structural and functional changes occurring in the olfactory mucosa caused by inflammation. However, the current understanding of the pathophysiology of olfactory dysfunction in allergic rhinitis remains unclear. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/cea.13401
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http://dx.doi.org/10.1111/cea.13401DOI Listing
April 2019
5 Reads

Manufacturing and characterization of extracellular vesicles from umbilical cord-derived mesenchymal stromal cells for clinical testing.

Cytotherapy 2019 Apr 9. Epub 2019 Apr 9.

GMP Laboratory, Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University (PMU), Salzburg, Austria; Department of Transfusion Medicine, Paracelsus Medical University (PMU), Salzburg, Austria; Research Program Nanovesicular Therapies, Paracelsus Medical University (PMU), Salzburg, Austria; Celericon Therapeutics G.m.b.H., Paracelsus Medical University (PMU), Salzburg, Austria.

Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) may deliver therapeutic effects that are comparable to their parental cells. MSC-EVs are promising agents for the treatment of a variety of diseases. To reach the intermediate goal of clinically testing safety and efficacy of EVs, strategies should strive for efficient translation of current EV research. Read More

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http://dx.doi.org/10.1016/j.jcyt.2018.12.006DOI Listing

Sex-based dimorphisms in expression of BDNF and BACE1 in bipolar patients.

Compr Psychiatry 2019 Mar 18. Epub 2019 Mar 18.

Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Bipolar disorder (BD) is a chronic, serious mental disorder distinguished by repeated episodes of mania and depression. Previous studies have demonstrated dysregulation of a number of transcripts in brain tissue or peripheral blood of BD patients. In the present study, we compared expression of two protein coding genes (brain-derived neurotrophic factor (BDNF) and beta-secretase 1 (BACE1)) and their natural occurring anti-sense (AS) RNAs (BDNF-AS and BACE1-AS) in peripheral blood of 50 BD patients (mean age ± standard deviation (SD) = 36. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0010440X193001
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http://dx.doi.org/10.1016/j.comppsych.2019.02.008DOI Listing
March 2019
2 Reads

Mesenchymal stem cells: from roots to boost.

Stem Cells 2019 Apr 12. Epub 2019 Apr 12.

NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.

It was shown as long as half a century ago that bone marrow is a source of not only hematopoietic stem cells, but also stem cells of mesenchymal tissues. Then the term of mesenchymal stem cells (MSCs) has been coined in early 1990s and over a decade later the criteria for defining MSCs have been released by International Society for Cellular Therapy. The easy derivation from a variety of fetal and adult tissues and not demanding cell culture conditions made MSCs an attractive research object. Read More

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http://doi.wiley.com/10.1002/stem.3016
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http://dx.doi.org/10.1002/stem.3016DOI Listing
April 2019
2 Reads

Sub-confluent culture of human mesenchymal stromal cells on biodegradable polycaprolactone microcarriers enhances bone healing of rat calvarial defect.

Cytotherapy 2019 Apr 8. Epub 2019 Apr 8.

Stem Cell Group 2 Bioprocessing Technology Institute, Agency of Science, Technology and Research (A*STAR), Singapore.

In the current emerging trend of using human mesenchymal stromal cell (MSCs) for cell therapy, large quantities of cells are needed for clinical testing. Current methods of culturing cells, using tissue culture flasks or cell multilayer vessels, are proving to be ineffective in terms of cost, space and manpower. Therefore, alternatives such as large-scale industrialized production of MSCs in stirred tank bioreactors using microcarriers (MCs) are needed. Read More

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http://dx.doi.org/10.1016/j.jcyt.2019.03.004DOI Listing
April 2019
2 Reads

Empowering Mesenchymal Stem Cells for Ocular Degenerative Disorders.

Int J Mol Sci 2019 Apr 10;20(7). Epub 2019 Apr 10.

Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

Multipotent mesenchymal stem cells (MSCs) have been employed in numerous pre-clinical and clinical settings for various diseases. MSCs have been used in treating degenerative disorders pertaining to the eye, for example, age-related macular degeneration, glaucoma, retinitis pigmentosa, diabetic retinopathy, and optic neuritis. Despite the known therapeutic role and mechanisms of MSCs, low cell precision towards the targeted area and cell survivability at tissue needing repair often resulted in a disparity in therapeutic outcomes. Read More

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http://dx.doi.org/10.3390/ijms20071784DOI Listing
April 2019
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Lung tissue engineering: An update.

J Cell Physiol 2019 Apr 10. Epub 2019 Apr 10.

Nanotechnology and Regenerative Medicine Commercialization Centre (Ltd), The London Bioscience Innovation Centre, London, UK.

Pulmonary disease is a worldwide public health problem that reduces the life quality and increases the need for hospital admissions as well as the risk of premature death. A common problem is the significant shortage of lungs for transplantation as well as patients must also take immunosuppressive drugs for the rest of their lives to keep the immune system from attacking transplanted organs. Recently, a new strategy has been proposed in the cellular engineering of lung tissue as decellularization approaches. Read More

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http://dx.doi.org/10.1002/jcp.28558DOI Listing
April 2019
4 Reads
3.839 Impact Factor

Repurposing vitamin D for treatment of human malignancies targeting tumor microenvironment.

Acta Pharm Sin B 2019 Mar 6;9(2):203-219. Epub 2018 Sep 6.

Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou 646000, China.

Tumor cells along with a small proportion of cancer stem cells exist in a stromal microenvironment consisting of vasculature, cancer-associated fibroblasts, immune cells and extracellular components. Recent epidemiological and clinical studies strongly support that vitamin D supplementation is associated with reduced cancer risk and favorable prognosis. Experimental results suggest that vitamin D not only suppresses cancer cells, but also regulates tumor microenvironment to facilitate tumor repression. Read More

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http://dx.doi.org/10.1016/j.apsb.2018.09.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437556PMC
March 2019
1 Read

Transplant center practices for psychosocial assessment and management of pediatric hematopoietic stem cell donors.

Bone Marrow Transplant 2019 Apr 10. Epub 2019 Apr 10.

Departments of Medicine, Psychiatry, Clinical and Translational Science, University of Pittsburgh, Pittsburgh, PA, USA.

Understanding the potential emotional and psychological risks of pediatric sibling HSC donation is an area of research that remains in its infancy. A cross-sectional survey was distributed electronically to directors at all CIBMTR and EBMT centers to describe current transplant center practices for obtaining assent, preparation for the physical/emotional experiences of donation, and monitoring the post-donation well-being of pediatric donors (<18 years of age). Respondents were 45/91 (49%) and 66/144 (46%) of CIBMTR and EBMT centers, respectively. Read More

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http://www.nature.com/articles/s41409-019-0515-3
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http://dx.doi.org/10.1038/s41409-019-0515-3DOI Listing
April 2019
8 Reads

Epidemiology, Diagnosis, Treatment, and Prevention of Influenza Infection in Oncology Patients.

J Oncol Pract 2019 Apr;15(4):177-184

1 University of Nebraska Medical Center, Omaha, NE.

Influenza infection causes increased morbidity and higher mortality in patients receiving treatment of underlying cancer, particularly in those with hematological malignancy or patients who have undergone hematopoietic stem-cell transplantation. The illness is characterized by seasonality and nonspecific clinical manifestations of upper respiratory infection at a time when other respiratory illnesses are common in the community, making the diagnosis challenging. However, accurate and timely diagnosis by new molecular techniques is crucial in the management of immunocompromised patients, because delays in initiating appropriate therapy can have devastating consequences. Read More

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http://dx.doi.org/10.1200/JOP.18.00567DOI Listing
April 2019
1 Read

The role of satellite and other functional cell types in muscle repair and regeneration.

J Muscle Res Cell Motil 2019 Apr 9. Epub 2019 Apr 9.

College of Animal Sciences, Zhejiang University; The Key Laboratory of Molecular Animal Nutrition, Ministry of Education; Zhejiang Provincial Laboratory of Feed and Animal Nutrition, No. 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, People's Republic of China.

Skeletal muscles play essential roles in physiological processes, including motor function, energy hemostasis, and respiration. Skeletal muscles also have the capacity to regenerate after injury. Regeneration of skeletal muscle is an extremely complex biological process, which involves multiple cell types. Read More

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http://dx.doi.org/10.1007/s10974-019-09511-3DOI Listing

The Impact of Natural Antioxidants on the Regenerative Potential of Vascular Cells.

Front Cardiovasc Med 2019 22;6:28. Epub 2019 Mar 22.

King Abdullah International Medical Research Centre, King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard-Health Affairs, King Abdulaziz Medical City, Jeddah, Saudi Arabia.

With advances in technology, the impact of natural antioxidants on vascular cell regeneration is attracting enormous attention as many current studies are now exploring the clinical potential of antioxidants in regenerative medicine. Natural antioxidants are an important step for improving future treatment and prevention of various diseases such as cardiovascular, cancer, neurodegenerative, and diabetes. The use of natural antioxidants which have effects on several types of stem cells with the potential to differentiate into functional endothelium and smooth muscle cells (known as vascular progenitors) for vascular regeneration might override pharmaceutical and surgical treatments. Read More

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http://dx.doi.org/10.3389/fcvm.2019.00028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439348PMC

Investigating the Complex Arrhythmic Phenotype Caused by the Gain-of-Function Mutation KCNQ1-G229D.

Front Physiol 2019 18;10:259. Epub 2019 Mar 18.

The William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom.

The congenital long QT syndrome (LQTS) is a cardiac electrophysiological disorder that can cause sudden cardiac death. LQT1 is a subtype of LQTS caused by mutations in KCNQ1, affecting the slow delayed-rectifier potassium current (), which is essential for cardiac repolarization. Paradoxically, gain-of-function mutations in KCNQ1 have been reported to cause borderline QT prolongation, atrial fibrillation (AF), sinus bradycardia, and sudden death, however, the mechanisms are not well understood. Read More

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http://dx.doi.org/10.3389/fphys.2019.00259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6430739PMC
March 2019
1 Read

Adapting Cord Blood Collection and Banking Standard Operating Procedures for HLA-Homozygous Induced Pluripotent Stem Cells Production and Banking for Clinical Application.

J Clin Med 2019 Apr 8;8(4). Epub 2019 Apr 8.

Banc de Sang i Teixits, Edifici Dr. Frederic Duran i Jordà, Passeig Taulat 116, 08005 Barcelona, Spain.

In this article, we will discuss the main aspects to be considered to define standard operation procedures (SOPs) for the creation of an induced pluripotent stem cell (iPSC) bank using cord blood (CB)-or similar cell type-bank guidelines for clinical aims. To do this, we adapt the pre-existing SOP for CB banking that can be complementary for iPSCs. Some aspects of iPSC manufacturing and the particular nature of these cells call for special attention, such as the potential multiple applications of the cells, proper explanation to the donor for consent of use, the genomic stability and the risk of genetic privacy disclosure. Read More

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http://dx.doi.org/10.3390/jcm8040476DOI Listing

A Therapeutic Strategy for Chemotherapy-Resistant Gastric Cancer via Destabilization of Both β-Catenin and RAS.

Cancers (Basel) 2019 Apr 8;11(4). Epub 2019 Apr 8.

Translational Research Center for Protein Function Control, Yonsei University, Seoul 03722, Korea.

Treatment of advanced gastric cancer patients with current standard chemotherapeutic agents frequently results in resistance, leading to poor overall survival. However, there has been no success in developing strategies to overcome it. We showed the expression levels of both β-catenin and were significantly increased and correlated in tissues of 756 gastric cancer (GC) patients and tissues of primary- and acquired-resistance patient-derived xenograft tumors treated with 5-fluorouracil and oxaliplatin modulated with leucovorin (FOLFOX). Read More

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https://www.mdpi.com/2072-6694/11/4/496
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http://dx.doi.org/10.3390/cancers11040496DOI Listing
April 2019
8 Reads

Engineering three-dimensional microenvironments towards in vitro disease models of the central nervous system.

Biofabrication 2019 Apr 9. Epub 2019 Apr 9.

Biomedical Engineering, Hong Kong Polytechnic University, Kowloon, HONG KONG.

The central nervous system (CNS) has a highly complex biophysical and biochemical environment. Despite decades of intensive research, it is still an enormous challenge to restore its functions and regenerate lost or damaged CNS tissues. Current treatment strategies remain sub-optimal because of (1) the hostile microenvironment created post CNS injury, and (2) insufficient understanding of the pathophysiology of acute and chronic CNS diseases. Read More

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http://dx.doi.org/10.1088/1758-5090/ab17aaDOI Listing
April 2019
6 Reads

Modulation of lipolysis and glycolysis pathways in cancer stem cells changed multipotentiality and differentiation capacity toward endothelial lineage.

Cell Biosci 2019 27;9:30. Epub 2019 Mar 27.

2Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Cancer stem cells obtain energy demand through the activation of glycolysis and lipolysis. It seems that the use of approached targeting glycolysis and lipolysis could be an effective strategy for the inhibition of cancer stem cells. In the current experiment, we studied the potential effect of glycolysis and lipolysis inhibition on cancer stem cells differentiation and mesenchymal-epithelial-transition capacity. Read More

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http://dx.doi.org/10.1186/s13578-019-0293-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437852PMC
March 2019
1 Read
3.210 Impact Factor

<Editors' Choice> Meddling with meddlers: curbing regulatory T cells and augmenting antitumor immunity.

Nagoya J Med Sci 2019 Feb;81(1):1-18

Department of Immunology, Nagoya University Graduate School of Medicine, Nagoya, Japan.

CD4 regulatory T cells (Tregs) expressing the transcription factor forkhead box P3 (FoxP3) play an important role in self-tolerance and immune homeostasis. Tregs have evolved to protect the host from aberrant immune responses against self-components and collateral damages occurring in the process of defense against invading pathogens by softening immune responses. However, they turned to be a scourge in malignant tumors by not only allowing and promoting tumor growth but also suppressing effective antitumor actions, both inherent (host's immune surveillance) and extrinsic (anticancer therapy). Read More

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http://dx.doi.org/10.18999/nagjms.81.1.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6433633PMC
February 2019

Research on the Methods for the Mass Production of Multi-Scale Organs-On-Chips.

Polymers (Basel) 2018 Nov 7;10(11). Epub 2018 Nov 7.

Departamento de Biología Molecular, Universidad Autónoma de Madrid, 28049 Cantoblanco-Madrid, Spain.

The success of labs- and organs-on-chips as transformative technologies in the biomedical arena relies on our capacity of solving some current challenges related to their design, modeling, manufacturability, and usability. Among present needs for the industrial scalability and impact promotion of these bio-devices, their sustainable mass production constitutes a breakthrough for reaching the desired level of repeatability in systematic testing procedures based on labs- and organs-on-chips. The use of adequate biomaterials for cell-culture processes and the achievement of the multi-scale features required, for in vitro modeling the physiological interactions among cells, tissues, and organoids, which prove to be demanding requirements in terms of production. Read More

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http://dx.doi.org/10.3390/polym10111238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401721PMC
November 2018
2 Reads

Recent Advances in Engineered Stem Cell-Derived Cell Sheets for Tissue Regeneration.

Polymers (Basel) 2019 Jan 26;11(2). Epub 2019 Jan 26.

Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan-si, Chungcheongnam-do 31151, Korea.

The substantial progress made in the field of stem cell-based therapy has shown its significant potential applications for the regeneration of defective tissues and organs. Although previous studies have yielded promising results, several limitations remain and should be overcome for translating stem cell-based therapies to clinics. As a possible solution to current bottlenecks, cell sheet engineering (CSE) is an efficient scaffold-free method for harvesting intact cell sheets without the use of proteolytic enzymes, and may be able to accelerate the adoption of stem cell-based treatments for damaged tissues and organs regeneration. Read More

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http://dx.doi.org/10.3390/polym11020209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419010PMC
January 2019
2.505 Impact Factor

Opportunities and challenges for the use of induced pluripotent stem cells in modelling neurodegenerative disease.

Open Biol 2019 Jan;9(1):180177

1 Institute of Cellular and Organismic Biology, Academia Sinica , Taipei 11529 , Taiwan, Republic of China.

Adult-onset neurodegenerative diseases are among the most difficult human health conditions to model for drug development. Most genetic or toxin-induced cell and animal models cannot faithfully recapitulate pathology in disease-relevant cells, making it excessively challenging to explore the potential mechanisms underlying sporadic disease. Patient-derived induced pluripotent stem cells (iPSCs) can be differentiated into disease-relevant neurons, providing an unparalleled platform for in vitro modelling and development of therapeutic strategies. Read More

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http://dx.doi.org/10.1098/rsob.180177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367134PMC
January 2019
1 Read

Exploring and Exploiting Tissue Engineering Through the Design of Multifunctional Therapeutic Systems.

Authors:
Masoud Mozafari

Curr Stem Cell Res Ther 2019 ;14(2):80-82

Materials and Energy Research Center (MERC) Cellular and Molecular Research Center Iran University of Medical Sciences (IUMS) Tehran, Iran.

This article presents a special issue of "Current Stem Cell Research & Therapy" devoted to exploring and exploiting tissue engineering through the design of multifunctional therapeutic systems. This lead article draws from twelve contributed articles to discuss the most recent advancements in this emerging field. The common theme in the contributed articles is the emerging therapeutic strategies, and a special appeal is made for collaboration between engineers and biologists for the development of multifunctional therapeutic systems for tissue engineering and regenerative medicine. Read More

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http://dx.doi.org/10.2174/1574888X1402190218113533DOI Listing
January 2019
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Fibrin hydrogel as a scaffold for differentiation of induced pluripotent stem cells into oligodendrocytes.

J Biomed Mater Res B Appl Biomater 2019 Apr 8. Epub 2019 Apr 8.

Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

The importance of tissue engineering has been established as a promising approach in treating neurodegenerative diseases. The purpose of the current study is to determine the effect of fibrin hydrogel on the differentiation of iPSC into oligodendrocyte. For this purpose, iPSCs transduced by miR-338 expressing lentiviruses. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.b.34378
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http://dx.doi.org/10.1002/jbm.b.34378DOI Listing
April 2019
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Pharmacological blockade of the CD39/CD73 pathway but not adenosine receptors augments disease in a humanised mouse model of graft-versus-host disease.

Immunol Cell Biol 2019 Apr 7. Epub 2019 Apr 7.

School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW, 2252, Australia.

Allogeneic haematopoietic stem cell transplantation (HSCT) is a curative therapy for a number of haematological malignancies, but is limited by the development of graft-versus-host disease (GVHD). CD39 and CD73 form an ecto-enzymatic pathway that hydrolyses extracellular adenosine 5'-triphosphate (ATP) to adenosine, which respectively exacerbate or alleviate disease in allogeneic mouse models of GVHD. The current study aimed to explore the role of the CD39/CD73 pathway and adenosine receptor (AR) blockade in a humanised mouse model of GVHD. Read More

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http://dx.doi.org/10.1111/imcb.12251DOI Listing
April 2019
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Expression of long noncoding RNAs in breast cancer in relation to reproductive factors and tumor characteristics.

J Cell Biochem 2019 Apr 7. Epub 2019 Apr 7.

Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Long noncoding RNAs (lncRNA) regulate expressions of several cancer-related genes and pathways. Expression profiling of lncRNAs would pave the way for identification of dysregulated pathways in cancer. We assessed expression of FAS-anti sense 1 (FAS-AS1), Taurine Upregulated 1 (TUG1), OPA-interacting protein 5-AS1 (OIP5 -AS1), nuclear-enriched abundant transcript 1 (NEAT1), and highly upregulated in liver cancer (HULC) in breast tumor tissues and adjacent noncancerous tissues (ANCTs). Read More

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http://dx.doi.org/10.1002/jcb.28671DOI Listing

Mesenchymal stromal cells for bone sarcoma treatment: Roadmap to clinical practice.

J Bone Oncol 2019 Jun 19;16:100231. Epub 2019 Mar 19.

Academic Orthopaedic Unit, Papageorgiou General Hospital, Aristotle University Medical School, West Ring Road of Thessaloniki, Pavlos Melas Area, N. Efkarpia, 56403 Thessaloniki, Greece.

Over the past few decades, there has been growing interest in understanding the molecular mechanisms of cancer pathogenesis and progression, as it is still associated with high morbidity and mortality. Current management of large bone sarcomas typically includes the complex therapeutic approach of limb salvage or sacrifice combined with pre- and postoperative multidrug chemotherapy and/or radiotherapy, and is still associated with high recurrence rates. The development of cellular strategies against specific characteristics of tumour cells appears to be promising, as they can target cancer cells selectively. Read More

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http://dx.doi.org/10.1016/j.jbo.2019.100231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434099PMC
June 2019
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Expansion of Murine MSC Impairs Transcription Factor-Induced Differentiation into Pancreatic -Cells.

Stem Cells Int 2019 10;2019:1395301. Epub 2019 Mar 10.

The School of Life Sciences and Centre for Health Technologies, Faculty of Science, University of Technology Sydney, Sydney, Australia.

Combinatorial gene and cell therapy as a means of generating surrogate -cells has been investigated for the treatment of type 1 diabetes (T1D) for a number of years with varying success. One of the limitations of current cell therapies for T1D is the inability to generate sufficient quantities of functional transplantable insulin-producing cells. Due to their impressive immunomodulatory properties, in addition to their ease of expansion and genetic modification , mesenchymal stem cells (MSCs) are an attractive alternative source of adult stem cells for regenerative medicine. Read More

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http://dx.doi.org/10.1155/2019/1395301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431458PMC

Complex Formation between VEGFR2 and the β-Adrenoceptor.

Cell Chem Biol 2019 Mar 14. Epub 2019 Mar 14.

Division of Physiology, Pharmacology & Neuroscience, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; Centre of Membrane Proteins and Receptors, University of Birmingham and University of Nottingham, The Midlands, UK. Electronic address:

Vascular endothelial growth factor (VEGF) is an important mediator of endothelial cell proliferation and angiogenesis via its receptor VEGFR2. A common tumor associated with elevated VEGFR2 signaling is infantile hemangioma that is caused by a rapid proliferation of vascular endothelial cells. The current first-line treatment for infantile hemangioma is the β-adrenoceptor antagonist, propranolol, although its mechanism of action is not understood. Read More

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http://dx.doi.org/10.1016/j.chembiol.2019.02.014DOI Listing
March 2019
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Human pluripotent stem cell-derived cardiomyocytes for studying energy metabolism.

Biochim Biophys Acta Mol Cell Res 2019 Apr 4. Epub 2019 Apr 4.

University Medical Center Hamburg-Eppendorf, Institute of Experimental Pharmacology and Toxicology, 20246 Hamburg, Germany; German Centre for Heart Research (DZHK), Partner Site Hamburg/Kiel/Lübeck, Hamburg, Germany. Electronic address:

Cardiomyocyte energy metabolism is altered in heart failure, and primary defects of metabolic pathways can cause heart failure. Studying cardiac energetics in rodent models has principal shortcomings, raising the question to which extent human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CM) can provide an alternative. As metabolic maturation of CM occurs mostly after birth during developmental hypertrophy, the immaturity of hiPSC-CM is an important limitation. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2019.04.001DOI Listing