261,479 results match your criteria Current Protein & Peptide Science[Journal]


Ascl1 Regulates Electric Field-Induced Neuronal Differentiation through PI3K/Akt Pathway.

Neuroscience 2019 Feb 13. Epub 2019 Feb 13.

Department of Histology & Embryology, College of Basic Medical Sciences, Jilin University, Changchun 130021, PR China. Electronic address:

Directing differentiation of neural stem/progenitor cells (NSCs/NPCs) to produce functional neurons is one of the greatest challenging in regenerative medicine. Our previous paper has confirmed that electrical stimulation has a high efficiency of triggering neuronal differentiation by using isolated filum terminale (FT) derived NPCs. To further clarify the intrinsic molecular mechanisms, protein-protein interaction (PPI) network analysis was applied to pinpoints novel hubs in electric field (EF)-induced neuronal differentiation. Read More

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http://dx.doi.org/10.1016/j.neuroscience.2019.02.004DOI Listing
February 2019

FMRpolyG alters mitochondrial transcripts level and respiratory chain complex assembly in Fragile X associated tremor/ataxia syndrome [FXTAS].

Biochim Biophys Acta Mol Basis Dis 2019 Feb 13. Epub 2019 Feb 13.

Department of Biochemistry, Faculty of Science, The M.S. University of Baroda, Vadodara 390002, Gujarat, India. Electronic address:

Fragile X-associated tremor/ataxia syndrome (FXTAS) is an inherited neurodegenerative disorder caused by an expansion of 55 to 200 CGG repeats (premutation) in FMR1. These CGG repeats are Repeat Associated non-ATG (RAN) translated into a small and pathogenic protein, FMRpolyG. The cellular and molecular mechanisms of FMRpolyG toxicity are unclear. Read More

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http://dx.doi.org/10.1016/j.bbadis.2019.02.010DOI Listing
February 2019

Production of neutralizing antibodies against the secreted Clostridium chauvoei toxin A (CctA) upon blackleg vaccination.

Anaerobe 2019 Feb 13. Epub 2019 Feb 13.

Vetuisse Faculty, University of Bern, Bern, Switzerland. Electronic address:

Clostridium chauvoei is the etiologic agent of blackleg in cattle, inducing fever, severe myonecrosis, oedemic lesions and ultimately death of infected animals. The pathogen often results in such rapid death that antibiotic therapy is futile and thus vaccination is the only efficient strategy in order to control the disease. The β-barrel pore forming leucocidin Clostridium chauvoei toxin A (CctA) is one of the best characterised toxins of C. Read More

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http://dx.doi.org/10.1016/j.anaerobe.2019.02.011DOI Listing
February 2019

Localized Surface Plasmon Resonance Biosensing of Tomato Yellow Leaf Curl Virus.

J Virol Methods 2019 Feb 13. Epub 2019 Feb 13.

Department of biotechnology and plant breeding, faculty of agriculture, Ferdowsi university of Mashhad, Iran.

Current techniques for plant virus detection, such as RT- PCR and ELISA, require multistep procedures and rely on sophisticated equipment. Due to the global spread of plant viruses, the development of simpler, faster and cheaper assay methods is inevitable. Gold nanoparticles (AuNPs) had raised much interest during recent years due to their novel optical properties or diagnostic purposes. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.02.004DOI Listing
February 2019

BAG3 deletion suppresses stem cell-like features of pancreatic ductal adenocarcinoma via translational suppression of ISG15.

Biochim Biophys Acta Mol Cell Res 2019 Feb 13. Epub 2019 Feb 13.

Department of Biochemistry & Molecular Biology, China Medical University, Shenyang 110122, China; Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang 110122, China. Electronic address:

BAG3 is a member of the cochaperone BAG family and often highly expressed in various cancers. Recently, evidences show that BAG3 promotes stemness of human cancer cells. IFN-stimulated genes 15 (ISG15) is an ubiquitin-like molecule, which is covalently conjugated with substrates to form ISGylated proteins. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2019.02.008DOI Listing
February 2019

Modulation of neuroplasticity-related targets following stress-induced acute escape deficit.

Behav Brain Res 2019 Feb 13. Epub 2019 Feb 13.

Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125, Modena, Italy; Center for Neuroscience and Neurotechnology, University of Modena and Reggio Emilia, Modena, Italy. Electronic address:

Understanding resilience is a major challenge to improve current pharmacological therapies aimed at complementing psychological-based approaches of stress-related disorders. In particular, resilience is a multi-factorial construct where the complex network of molecular events that drive the process still needs to be resolved. Here, we exploit the Acute Escape Deficit model, an animal model based on exposure to acute unavoidable stress followed by an escape test, to define vulnerable and resilient phenotypes in rats. Read More

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http://dx.doi.org/10.1016/j.bbr.2019.02.023DOI Listing
February 2019

Biochemical characterization of TyrA dehydrogenases from Saccharomyces cerevisiae (Ascomycota) and Pleurotus ostreatus (Basidiomycota).

Arch Biochem Biophys 2019 Feb 13. Epub 2019 Feb 13.

Department of Botany, University of Wisconsin-Madison, Madison, WI, 53706, USA.

L-Tyrosine is an aromatic amino acid necessary for protein synthesis in all living organisms and a precursor of secondary (specialized) metabolites. In fungi, tyrosine-derived compounds are associated with virulence and defense (i.e. Read More

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http://dx.doi.org/10.1016/j.abb.2019.02.005DOI Listing
February 2019

Comparative Genomics, Siderophore Production, and Iron Scavenging Potential of Root Zone Soil Bacteria Isolated from 'Concord' Grape Vineyards.

Microb Ecol 2019 Feb 15. Epub 2019 Feb 15.

Department of Crop and Soil Sciences, Washington State University, PO Box 646420, Pullman, WA, USA.

Iron (Fe) deficiency in crop production is a worldwide problem which often results in chlorosis in grapevines, particularly in calcareous soils. Siderophores secreted by microorganisms and Strategy II plants can chelate Fe and other metals in soil solution, and siderophore-Fe complexes can then be utilized by plants and microbes. Plants may also shift rhizosphere conditions to favor siderophore-producing microbes, which can increase plant available Fe. Read More

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http://dx.doi.org/10.1007/s00248-019-01324-8DOI Listing
February 2019

PLOD3 suppression exerts an anti-tumor effect on human lung cancer cells by modulating the PKC-delta signaling pathway.

Cell Death Dis 2019 Feb 15;10(3):156. Epub 2019 Feb 15.

Division of Radiation Biomedical Research, Korea Institute of Radiological & Medical Sciences, Seoul, 01812, Korea.

Current lung cancer treatments are far from satisfactory; thus, finding novel treatment targets is crucial. We recently identified procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (PLOD3), which is involved in fibrosis and tissue remodeling as a radioresistance-related protein in lung cancer cells; however, its mechanism is unclear. In this study, we designed human PLOD3-specific short interfering (si)RNAs and tested their effects on tumor growth inhibition in vitro and in vivo. Read More

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http://dx.doi.org/10.1038/s41419-019-1405-8DOI Listing
February 2019

CaMKII Does not Control Mitochondrial Ca Uptake in Cardiac Myocytes.

J Physiol 2019 Feb 16. Epub 2019 Feb 16.

Comprehensive Heart Failure Center (CHFC), University Clinic Würzburg, 97078, Würzburg, Germany.

Key Points: Mitochondrial Ca uptake stimulates the Krebs cycle to regenerate the reduced forms of pyridine nucleotides (NADH, NADPH and FADH ) required for ATP production and ROS elimination. It was previously proposed that Ca /calmodulin-dependent protein kinase II (CaMKII) regulates mitochondrial Ca uptake via MCU phosphorylation. We used two mouse models with either global deletion of CaMKIIδ (CaMKIIδ KO) or cardiomyocyte-specific deletion of CaMKIIδ and γ (CaMKIIδ/γ DKO) to interrogate whether CaMKII controls mitochondrial Ca uptake in isolated mitochondria and during β-adrenergic stimulation in cardiac myocytes. Read More

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http://dx.doi.org/10.1113/JP276766DOI Listing
February 2019

Ras-PI3K-AKT signaling promotes the occurrence and development of uveal melanoma by downregulating H3K56ac expression.

J Cell Physiol 2019 Feb 15. Epub 2019 Feb 15.

Department of Vascular Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin, China.

Background: Uveal melanoma (UM) is an intraocular malignant tumor characterized by rapid progression and recurrence. The current conventional treatments are unsatisfactory. Histone acetylation at H3 lysine 56 (H3K56ac) has been reported to be a tumor suppressor in breast cancer. Read More

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http://dx.doi.org/10.1002/jcp.28261DOI Listing
February 2019

High Hydrostatic Pressure Processing Better Preserves the Nutrient and Bioactive Compound Composition of Human Donor Milk.

J Nutr 2019 Feb 15. Epub 2019 Feb 15.

Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, Canada.

Background: When mother's milk is insufficient, pasteurized human donor milk (DM) is the recommended supplement for hospitalized very-low-birth-weight infants. The current method of pasteurization (Holder, 62.5°C, 30 min) negatively affects heat-sensitive nutrients and bioactive proteins. Read More

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http://dx.doi.org/10.1093/jn/nxy302DOI Listing
February 2019

Sexual quality of life in patients with axial spondyloarthritis in the biologic treatment era.

J Rheumatol 2019 Feb 15. Epub 2019 Feb 15.

Faculty of Health and Sport, University of Agder, Norway. Department of Rheumatology, Sorlandet Hospital HF, Kristiansand, Department of Rheumatology, Martina Hansens Hospital, Bærum, Department of Neuroscience, Division of Rheumatology, Norwegian University of Science and Technology, Trondheim, Norway. Address correspondence to Kari Hansen Berg, PhD student and Head of Institute of Nursing Sciences, Faculty of Health and Sport, University of Agder, Norway, Postbox 422, 4604 Kristiansand Phone: 0047 93037669 E-mail:

Objective: To explore the relationship between demographic, disease related variables, treatment and sexual quality of life (SQOL) in men and women with Axial Spondyloarthritis (ax-SpA).

Methods: Ax-SpA patients were consecutively recruited from two rheumatology outpatient clinics in Sothern Norway. A broad specter of demographic, disease, treatment and quality of life data were systematically collected. Read More

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http://dx.doi.org/10.3899/jrheum.180413DOI Listing
February 2019

The malate-activated ALMT12 anion channel in the grass is co-activated by Ca/calmodulin.

J Biol Chem 2019 Feb 15. Epub 2019 Feb 15.

University of Saskatchewan, Canada.

In plants, strict regulation of stomatal pores is critical for modulation of CO fixation and transpiration. Under certain abiotic and biotic stressors, pore closure is initiated through anionic flux, with calcium (Ca), playing a central role. The aluminum-activated malate transporter 12 (ALMT12) is a malate-activated, voltage-dependent member of the aluminum-activated malate transporter family, implicated in anionic flux from guard cells controlling stomatal aperture. Read More

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http://dx.doi.org/10.1074/jbc.RA118.005301DOI Listing
February 2019

Nanometer-accuracy distance measurements between fluorophores at the single-molecule level.

Proc Natl Acad Sci U S A 2019 Feb 15. Epub 2019 Feb 15.

Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158.

Light microscopy is a powerful tool for probing the conformations of molecular machines at the single-molecule level. Single-molecule Förster resonance energy transfer can measure intramolecular distance changes of single molecules in the range of 2 to 8 nm. However, current superresolution measurements become error-prone below 25 nm. Read More

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http://dx.doi.org/10.1073/pnas.1815826116DOI Listing
February 2019

Persistence and 4-year boosting of the bactericidal response elicited by two- and three-dose schedules of MenB-FHbp: A phase 3 extension study in adolescents.

Vaccine 2019 Feb 12. Epub 2019 Feb 12.

Pfizer Vaccine Research and Development, 500 Arcola Road, Collegeville, PA 19426, USA.

Background: The period of heightened risk of invasive meningococcal disease in adolescence extends for >10 years. This study aimed to evaluate persistence of the immune response to the serogroup B meningococcal (MenB) vaccine MenB-FHbp (Trumenba, Bivalent rLP2086) under two- and three-dose primary vaccination schedules, both of which are approved in the United States and the European Union, and to assess safety and immunogenicity of a booster dose.

Methods: This was an open-label extension study of a phase 2 randomized MenB-FHbp study (primary study). Read More

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http://dx.doi.org/10.1016/j.vaccine.2018.11.073DOI Listing
February 2019

Immunogenicity and protective potential of Bordetella pertussis biofilm and its associated antigens in a murine model.

Cell Immunol 2019 Jan 30. Epub 2019 Jan 30.

School of Pharmacy and Biomedical Sciences & Curtin Health Innovation Research Institute, Curtin University, Bentley, Western Australia, Australia; Department of Microbiology, Pathwest Laboratory Medicine, Fiona Stanley Hospital, Murdoch, Australia. Electronic address:

The resurgence of whooping cough reflects novel genetic variants of Bordetella pertussis and inadequate protection conferred by current acellular vaccines (aP). Biofilm is a source of novel vaccine candidates, including membrane protein assembly factor (BamB) and lipopolysaccharide assembly protein (LptD). Responses of BALB/c mice to candidate vaccines included IFN-γ and IL-17a production by spleen and lymph node cells, and serum IgG1 and IgG2a reactive with whole bacteria or aP. Read More

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http://dx.doi.org/10.1016/j.cellimm.2019.01.006DOI Listing
January 2019

Biomarker Analysis of Orally Dosed, Dual Active, Matrix Metalloproteinase (MMP)-2 and MMP-9 Inhibitor, AQU-118, in the Spinal Nerve Ligation (SNL) Rat Model of Neuropathic Pain.

Int J Mol Sci 2019 Feb 14;20(4). Epub 2019 Feb 14.

Aquilus Pharmaceuticals Inc., 3H Gill Street, Suite 300, Woburn, MA 01801, USA.

There is an unmet medical need for the development of non-addicting pain therapeutics with enhanced efficacy and tolerability. The current study examined the effects of AQU-118, an orally active inhibitor of metalloproteinase-2 (MMP-2) and MMP-9, in the spinal nerve ligation (SNL) rat model of neuropathic pain. Mechanical allodynia and the levels of various biomarkers were examined within the dorsal root ganglion (DRG) before and after oral dosing with AQU-118. Read More

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http://dx.doi.org/10.3390/ijms20040811DOI Listing
February 2019

Theaflavin-3,3'-Digallate Suppresses Human Ovarian Carcinoma OVCAR-3 Cells by Regulating the Checkpoint Kinase 2 and p27 kip1 Pathways.

Molecules 2019 Feb 14;24(4). Epub 2019 Feb 14.

College of Science, Technology and Mathematics, Alderson Broaddus University, Philippi, WV 26416, USA.

Theaflavin-3,3'-digallate (TF3) is a unique polyphenol in black tea. Epidemiological studies have proved that black tea consumption decreases the incidence rate of ovarian cancer. Our former research demonstrated that TF3 inhibited human ovarian cancer cells. Read More

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http://dx.doi.org/10.3390/molecules24040673DOI Listing
February 2019

Crigler-Najjar syndrome: therapeutic options and consequences of mutations in the UGT1A1 complex.

Expert Rev Endocrinol Metab 2008 Nov;3(6):725-737

b Paediatric Liver Centre, King's College London School of Medicine at King's College Hospital, Denmark Hill, London SE5 9PJ, UK.

Crigler-Najjar syndrome (CN), a rare inherited disorder characterized by failure of bilirubin glucuronidation, can lead to severe disability and death from kernicterus. Gilbert syndrome is a more common, benign familial unconjugated hyperbilirubinemia. The underlying problem in both conditions is impaired bilirubin conjugation and elimination due to a mutation in uridine 5'-diphosphate glucuronyltransferase. Read More

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http://dx.doi.org/10.1586/17446651.3.6.725DOI Listing
November 2008

Role of the aryl hydrocarbon receptor-interacting protein in familial isolated pituitary adenoma.

Expert Rev Endocrinol Metab 2010 Sep;5(5):681-695

a Department of Endocrinology, Barts and the London School of Medicine, Queen Mary University of London, EC1M 6BQ, UK.

Pituitary adenomas are typically sporadic benign tumors. However, approximately 5% of cases have been found to be familial in origin. Of these, approximately 40% occur in the absence of multiple endocrine neoplasia type 1 or Carney complex and have been termed 'familial isolated pituitary adenoma' (FIPA). Read More

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http://dx.doi.org/10.1586/eem.10.42DOI Listing
September 2010

Nutritional sensing and its utility in treating obesity.

Expert Rev Endocrinol Metab 2012 Mar;7(2):209-221

b Section of Investigative Medicine, Faculty of Medicine, Imperial College London, 6th Floor, Commonwealth Building, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK.

Obesity remains a major worldwide health problem, with current medical treatments being poorly effective. Nutrient sensing allows organs such as the GI tract and the brain to recognize and respond to fuel substrates such as carbohydrates, protein and fats. Specialized neural and hormonal pathways exist to facilitate and regulate these chemosensory mechanisms. Read More

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http://dx.doi.org/10.1586/eem.12.6DOI Listing

A comparison of the Mediterranean diet and current food consumption patterns in Spain from a nutritional and water perspective.

Sci Total Environ 2019 Feb 8;664:1020-1029. Epub 2019 Feb 8.

International Institute for Applied System Analysis (IIASA), Laxenburg, Austria.

The promotion of responsible consumption is a key strategy to achieve environmental benefits, sustainable food security, and enhance public health. Countries like Spain are making efforts to reverse growing obesity and promote healthy diets, such as the recommended and traditional Mediterranean, recognized as a key strategy to improve the population's health with locally grown, traditional, and seasonal products like fruits, vegetables, olive oil, and fish. With a view to connecting water, agriculture, food security, nutrition and health, this research aims to investigate and compare the nutritional and water implications of the current food consumption of Spanish households with the recommended Mediterranean diet. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2019.02.111DOI Listing
February 2019

Mechanisms and regulation of dopamine release.

Curr Opin Neurobiol 2019 Feb 12;57:46-53. Epub 2019 Feb 12.

Department of Neurobiology, Harvard Medical School, United States. Electronic address:

Dopamine controls motor functions, motivation, and reward-related learning through G-protein coupled receptor signaling. The current working model is that upon release, dopamine diffuses to influence many target cells via wide-spread receptors. Recent studies, however, suggest that dopamine release is fast and generates small signaling hotspots. Read More

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http://dx.doi.org/10.1016/j.conb.2019.01.001DOI Listing
February 2019

Comparison between Echinococcus granulosus sensu stricto (G1) and E. canadensis (G6) mitochondrial genes (cox1 and nad1) and their related protein models using experimental and bioinformatics analysis.

Comput Biol Chem 2019 Feb 5;79:103-109. Epub 2019 Feb 5.

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Cystic echinococcosis (CE) as a zoonotic parasitic disease, remains a health challenge in many parts of the world. There are different species of Echinococcus granulosus sensu lato with different pathogenicity and host preferences.Different procedures have been applied for characterization of Echinococcus taxa in which two mitochondrial genes, cox1 and nad1 have been used more common. Read More

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http://dx.doi.org/10.1016/j.compbiolchem.2019.02.002DOI Listing
February 2019

Retinal capillary perfusion: Spatial and temporal heterogeneity.

Prog Retin Eye Res 2019 Feb 12. Epub 2019 Feb 12.

Centre for Ophthalmology and Visual Science, The University of Western Australia, Perth, Australia; Lions Eye Institute, The University of Western Australia, Perth, Australia.

The central role of the cardiovascular system is to maintain adequate capillary perfusion. The spatially and temporally heterogeneous nature of capillary perfusion has been reported in some organs. However, such heterogeneous perfusion properties have not been sufficiently explored in the retina. Read More

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http://dx.doi.org/10.1016/j.preteyeres.2019.01.001DOI Listing
February 2019

Ras and exosome signaling.

Semin Cancer Biol 2019 Feb 12. Epub 2019 Feb 12.

Department of Oncology, Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI, USA. Electronic address:

Ras gene (HRAS, NRAS, and KRAS) has been observed to be mutated and hyper-activated in a significant proportion of cancers. However, mutant Ras remains a challenging therapeutic target. Similarly, inhibition of targets upstream and downstream of Ras has shown limited clinical utility. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S1044579X173022
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http://dx.doi.org/10.1016/j.semcancer.2019.02.004DOI Listing
February 2019
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A Multiepitopic theoretical fusion construct based on in-silico epitope screening of known vaccine candidates for protection against wide range of enterobacterial pathogens.

Hum Immunol 2019 Feb 12. Epub 2019 Feb 12.

Department of Microbiology, Panjab University, Chandigarh, India. Electronic address:

Enterobacterial pathogens that have acquired antibiotic resistance genes are a leading cause of community and hospital acquired infections. In such a situation vaccination is considered as a better option to prevent such infections. In the current study reverse vaccinology approach has been used to select peptides from already known immunogenic proteins to design a chimeric construct. Read More

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http://dx.doi.org/10.1016/j.humimm.2019.02.008DOI Listing
February 2019

Status of inflammation and alcohol use in a 6-month follow-up study of patients with major depressive disorder.

Alcohol 2019 Feb 12. Epub 2019 Feb 12.

University of Tampere, Faculty of Medicine and Life Sciences, 33014 University of Tampere, Finland and Seinäjoki Central Hospital, Department of Psychiatry, 60220 Seinäjoki, Finland.

Introduction: The studies on the relations between alcohol consumption, the status of inflammation and depression have produced conflicting results. In this study we followed patients with major depressive disorders by monitoring biomarkers of inflammation together with biomarkers of heavy alcohol use.

Method: The levels of IL-6 (interleukin-6), IL-8 (interleukin-8), hs-CRP (high sensitivity C-reactive protein), YKL-40 (also known as Chitinase-3-like protein 1 or CHI3L1) and biomarkers of alcohol consumption and liver status (GT, CDT, ALT, alkaline phosphatase) were measured at baseline and after 6 months of psychiatric treatment from 242 patients suffering from current major depressive disorder (MDD) with (n = 99) or without (n = 143) alcohol use disorder (AUD). Read More

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http://dx.doi.org/10.1016/j.alcohol.2019.02.001DOI Listing
February 2019

Application of Mass Spectrometry-Based Immunoassays for the Species- and Tissue-Specific Quantification of Banned Processed Animal Proteins in Feeds.

Anal Chem 2019 Feb 15. Epub 2019 Feb 15.

Processed Animal Proteins (PAPs) are considered as a sustainable protein source to improve the nutritional profile of feed for livestock and aquaculture. However, the use of these proteins is strongly regulated since the bovine spongiform encepha-lopathy (BSE) crisis. The reintroduction of non-ruminant PAPs for the use in aquaculture in 2013 has driven the need for alternative analytical methods to determine the species origin as well as the tissue source (legal or not). Read More

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http://dx.doi.org/10.1021/acs.analchem.8b04652DOI Listing
February 2019

[Lectins and glycoconjugates in presentation of antigens and protection against patogens.]

Klin Lab Diagn 2018 ;63(10):619-625

G.N. Gabrichevsky Research Institute for Epidemiology & Microbiology, Moscow, Russia.

The current data on using lectin receptors and glycoconjugates (GC) in study of pathways of intercellular communications within innate immunity network coupled to adaptive immunity wеre overviewed and systematized. A superfamily of GC-specific C-type lectin receptors (CLR), as a constituent of pattern recognizing receptors (PRR), involves in immunity to produce redistribution of groups of cytokines, chemokines and specific antibodies. CLR on surface of multiple subpopulations of antigen-presenting cells (APC) and other cells of immunity are under consideration (monocyte derived macrophages, macrophages, immature and mature dendritic cells, other myeloid cells, neutrophils, basophils, lymphocytes, etc. Read More

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http://dx.doi.org/10.18821/0869-2084-2018-63-10-619-625DOI Listing
January 2018
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Hyperpolarization-activated and cyclic nucleotide-gated channel proteins as emerging new targets in neuropathic pain.

Rev Neurosci 2019 Feb 15. Epub 2019 Feb 15.

Cancer Center, The First Hospital of Jilin University, 126 Xiantai Street, Changchun 130033, Jilin Province, China.

Hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels are activated during hyperpolarization, and there is an inward flow of current, which is termed as hyperpolarization-activated current, Ih. Initially, these channels were identified on the pacemaker cells of the heart. Nowadays, these are identified on different regions of the nervous system, including peripheral nerves, dorsal root ganglia, dorsal horns, and different parts of the brain. Read More

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http://dx.doi.org/10.1515/revneuro-2018-0094DOI Listing
February 2019

Dietary protein intake and kidney function decline after myocardial infarction: the Alpha Omega Cohort.

Nephrol Dial Transplant 2019 Feb 14. Epub 2019 Feb 14.

Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands.

Background: Post-myocardial infarction (MI) patients have a doubled rate of kidney function decline compared with the general population. We investigated the extent to which high intake of total, animal and plant protein are risk factors for accelerated kidney function decline in older stable post-MI patients.

Methods: We analysed 2255 post-MI patients (aged 60-80 years, 80% men) of the Alpha Omega Cohort. Read More

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http://dx.doi.org/10.1093/ndt/gfz015DOI Listing
February 2019

A Validated Score Assessing the Risk for an Intra-Abdominal Abscess in Patients with Crohn's Disease Presenting at the Emergency Department.

J Crohns Colitis 2019 Feb 14. Epub 2019 Feb 14.

Institute of Gastroenterology and Liver Diseases, Department of Medicine, Hadassah - Hebrew University Medical Center, Jerusalem, Israel.

Background And Aims: A majority of acutely-ill Crohn's disease (CD) patients who present to Emergency Department (ED) will undergo an abdominal CT to rule out disease complications. We aimed to generate a simple non-invasive scoring model to predict the presence of an intra-abdominal abscess in CD patients in the ED.

Methods: We performed a retrospective case control study at four Israeli hospitals from January-1 st-2010 to May-30 th-2018. Read More

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http://dx.doi.org/10.1093/ecco-jcc/jjz043DOI Listing
February 2019

Metabolites of C-reactive protein and vimentin are associated with disease activity of axial spondyloarthritis.

Clin Exp Rheumatol 2019 Feb 11. Epub 2019 Feb 11.

Institute of Rheumatology and Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic.

Objectives: Non-radiographic (nr-axSpA) and radiographic (AS) forms of axial spondyloarthritis (axSpA) share clinical features, but have different radiographic patterns. Radiographic progression is not associated with the current disease activity biomarkers. We investigated a matrix metalloproteinase mediated metabolite of C-reactive protein (CRPM) and two biomarkers of citrullinated vimentin (VICM and anti-MCV) as novel biomarkers of disease activity. Read More

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February 2019

IL-35 prevent bone loss through promotion of bone formation and angiogenesis in rheumatoid arthritis.

Clin Exp Rheumatol 2019 Feb 11. Epub 2019 Feb 11.

Department of Rheumatology and Immunology, the First Affiliated Hospital of China Medical University, Shenyang, China.

Objectives: Angiogenesis in bone and osteogenesis appear to be closely linked, suggesting the existence of molecular crosstalk between pro-angiogenic molecules and osteoblasts. The pro-angiogenic molecules vascular endothelial growth factor (VEGF) with its receptors Flt-1, Flk-1 and fibroblast growth factor (FGF)-2 play a crucial role in born formation, an early and critical event in the pathogenesis of rheumatoid arthritis (RA). Interleukin (IL)-35 is demonstrated to be an anti-inflammatory cytokine in RA. Read More

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February 2019

Separation, Characterization and Discriminant Analysis of Subvisible Particles in Biologics Formulations.

Curr Pharm Biotechnol 2019 Feb 13. Epub 2019 Feb 13.

BMS Research & Development, GRS&B, Princeton, New Jersey 08543. United States.

The presence of subvisible particles (SVPs) in parenteral formulations of biologics is a major challenge in the development of therapeutic protein formulations. Distinction between proteinaceous and non-proteinaceous SVPs is vital in monitoring formulation stability. The current compendial method based on light obscuration (LO) has limitations in the analysis of translucent/low refractive index particles. Read More

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http://dx.doi.org/10.2174/1389201020666190214100840DOI Listing
February 2019

Structure based Docking and Molecular dynamics studies: Peroxisome proliferator-activated receptors -α/γ dual agonists for treatment of metabolic disorders.

J Biomol Struct Dyn 2019 Feb 15:1-14. Epub 2019 Feb 15.

a Department of Pharmacy, School of Chemical Sciences and Pharmacy , Central University of Rajasthan , Ajmer , 301817 , India.

Diabetes is a foremost health problem globally susceptible to increased mortality and morbidity. The present therapies in the antidiabetic class have sound adverse effects and thus, emphasis on the further need to develop effective medication therapy. Peroxisome proliferator-activated receptor alpha-gamma dual approach represents an interesting target for developing novel anti-diabetic drug along with potential anti-hyperlipidimic activity. Read More

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http://dx.doi.org/10.1080/07391102.2019.1581089DOI Listing
February 2019

Nanostructured cochleates: A multi-layered platform for cellular transportation of therapeutics.

Drug Dev Ind Pharm 2019 Feb 15:1-37. Epub 2019 Feb 15.

a Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management SVKM's NMIMS , V. L. Mehta Road , Vile Parle (W), Mumbai , India.

Among lipid-based nanocarriers, multi-layered cochleates emerge as a novel delivery system because of prevention of oxidation of hydrophobic and hydrophilic drugs, enhancement in permeability and reduction in dose of drugs. It also improves oral bioavailability and increases the safety of a drug by targeting at a specific site with less side effects. Nanostructured cochleates are used as a carrier for the delivery of water-insoluble or hydrophobic drugs of anti-cancer, antiviral and anti-inflammatory action. Read More

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http://dx.doi.org/10.1080/03639045.2019.1583757DOI Listing
February 2019

Dynamin-like protein 1 cleavage by calpain in Alzheimer's disease.

Aging Cell 2019 Feb 14:e12912. Epub 2019 Feb 14.

Department of Pathology, Case Western Reserve University, Cleveland, Ohio.

Abnormal mitochondrial dynamics contributes to mitochondrial dysfunction in Alzheimer's disease (AD), yet the underlying mechanism remains elusive. In the current study, we reported that DLP1, the key mitochondrial fission GTPase, is a substrate of calpain which produced specific N-terminal DLP1 cleavage fragments. In addition, various AD-related insults such as exposure to glutamate, soluble amyloid-β oligomers, or reagents inducing tau hyperphosphorylation (i. Read More

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http://dx.doi.org/10.1111/acel.12912DOI Listing
February 2019

Rap1 signal modulators control the maintenance of hematopoietic progenitors in bone marrow and adult long-term hematopoiesis.

Cancer Sci 2019 Feb 15. Epub 2019 Feb 15.

Medical Innovation Center, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto, 606-8507, Japan.

Adult long-term hematopoiesis depends on sustaining hematopoietic stem/progenitor cells (HSPCs) in bone marrow (BM) niches, where their balance of quiescence, self-renewal, and hematopoietic differentiation is tightly regulated. While various BM stroma cells that produce niche factors have been identified, regulation of the intrinsic responsiveness of HSPCs to the niche factors remains elusive. We previously reported that mice deficient for Sipa1, a Rap1 GTPase-activating protein, develop diverse hematopoietic disorders of late onset. Read More

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http://dx.doi.org/10.1111/cas.13974DOI Listing
February 2019

A review of pig liver xenotransplantation: Current problems and recent progress.

Xenotransplantation 2019 Feb 15:e12497. Epub 2019 Feb 15.

Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.

Pig liver xenotransplantation appears to be more perplexing when compared to heart or kidney xenotransplantation, even though great progress has been achieved. The relevant molecular mechanisms involved in xenogeneic rejection, including coagulopathy, and particularly thrombocytopenia, are complex, and need to be systematically investigated. The deletion of expression of Gal antigens in the liver graft highlights the injurious impact of nonGal antigens, which continue to induce humoral rejection. Read More

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http://dx.doi.org/10.1111/xen.12497DOI Listing
February 2019

Progress in rapid optical assays for heavy metal ions based on the use of nanoparticles and receptor molecules.

Mikrochim Acta 2019 Feb 14;186(3):172. Epub 2019 Feb 14.

A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, Moscow, 119071, Russia.

This review (with 230 refs.) covers recent progress in rapid optical assays for heavy metals (primarily lead and mercury as the most relevant) based on the use of nanoparticles and receptor molecules. An introduction surveys the importance, regulatory demands (such as maximum permissible concentrations) and potential and limitations of various existing methods. Read More

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http://link.springer.com/10.1007/s00604-018-3168-9
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http://dx.doi.org/10.1007/s00604-018-3168-9DOI Listing
February 2019
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Mechanics of controlled release of insulin entrapped in polyacrylic acid gels via variable electrical stimuli.

Drug Deliv Transl Res 2019 Feb 14. Epub 2019 Feb 14.

Department of Biological and Agricultural Engineering, Texas A&M University, College Station, TX, USA.

Controlled release insulin delivery systems possess multiple advantages over conventional ones, including maintaining desired blood glucose levels for prolonged periods and minimizing complications due to insulin overdose. Compared to other controlled-release mechanisms, electro-responsive polymers present the advantages of high controllability and ability to be coupled with microelectronics. This paper reports the possibility of using electro-responsive polyacrylic acid (PAA) and polymethacrylic acid (PMA) hydrogels for controlled delivery of insulin using intermittent electrical signals via matrix deformation. Read More

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http://dx.doi.org/10.1007/s13346-019-00620-7DOI Listing
February 2019

Inhibitory effects of class I antiarrhythmic agents on Na and Ca currents of human iPS cell-derived cardiomyocytes.

Regen Ther 2019 Jun 1;10:104-111. Epub 2019 Feb 1.

Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Tottori University Graduate School of Medical Science, 86 Nishi-cho, Yonago 683-8503, Japan.

Introduction: Human induced pluripotent stem cells (hiPSCs) harboring cardiac myosin heavy chain 6 promoter can differentiate into functional cardiomyocytes called "iCell cardiomyocytes" under blasticidin treatment condition. While iCell cardiomyocytes are expected to be used for predicting cardiotoxicity of drugs, their responses to antiarrhythmic agents remain to be elucidated. We first examined electrophysiological properties of iCell cardiomyocytes and mRNA levels of ion channels and Ca handling proteins, and then evaluated effects of class I antiarrhythmic agents on their Na and Ca currents. Read More

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http://dx.doi.org/10.1016/j.reth.2018.12.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360514PMC

Rapid Production of Human VEGF-A following Intradermal Injection of Modified VEGF-A mRNA Demonstrated by Cutaneous Microdialysis in the Rabbit and Pig .

Biomed Res Int 2019 15;2019:3915851. Epub 2019 Jan 15.

Bioscience Heart Failure, Cardiovascular Renal and Metabolism, IMED Biotech Unit AstraZeneca Gothenburg, Sweden.

Chemically modified mRNA is a novel, highly efficient, biocompatible modality for therapeutic protein expression that may overcome the challenges and safety concerns with current gene therapy strategies. We explored the efficiency of intradermally injected modified VEGF-A mRNA (VEGF-A mRNA) formulated in a biocompatible citrate/saline buffer to locally produce human VEGF-A protein. Rabbits (n=4) and minipigs (n=3) were implanted with subcutaneous microdialysis probes close to the injection sites and interstitial-fluid samples and skin biopsies were analysed for production of VEGF-A protein over time for up to 8 hours. Read More

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http://dx.doi.org/10.1155/2019/3915851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350593PMC
January 2019

Modulating CRISPR gene drive activity through nucleocytoplasmic localization of Cas9 in .

Fungal Biol Biotechnol 2019 4;6. Epub 2019 Feb 4.

1Department of Biochemistry and Molecular Biophysics, 141 Chalmers Hall, Kansas State University, Manhattan, KS 66506 USA.

Background: The bacterial CRISPR/Cas genome editing system has provided a major breakthrough in molecular biology. One use of this technology is within a nuclease-based gene drive. This type of system can install a genetic element within a population at unnatural rates. Read More

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http://dx.doi.org/10.1186/s40694-019-0065-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360766PMC
February 2019

A PDZ Protein MDA-9/Syntenin: As a Target for Cancer Therapy.

Comput Struct Biotechnol J 2019 8;17:136-141. Epub 2019 Jan 8.

Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, PR China.

Melanoma differentiation-associated gene 9 (MDA-9)/Syntenin is a multidomain PDZ protein and identified as a key oncogene in melanoma initially. This protein contains a unique tandem PDZ domain architecture (PDZ1 and PDZ2 spaced by a 4-amino acid linker), an N-terminal domain (NTD) that is structurally uncharacterized and a short C-terminal domain (CTD). The PDZ1 domain is regarded as the PDZ signaling domain while PDZ2 served as the PDZ superfamily domain. Read More

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http://dx.doi.org/10.1016/j.csbj.2019.01.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360254PMC
January 2019

Constructing the Immune Signature of Schizophrenia for Clinical Use and Research; An Integrative Review Translating Descriptives Into Diagnostics.

Front Psychiatry 2018 31;9:753. Epub 2019 Jan 31.

Psychiatric Division, Haukeland University Hospital, Bergen, Norway.

Schizophrenia is considered a syndrome comprised by several disease phenotypes, covering a range of underlying pathologies. One of these disease mechanisms seems to involve immune dysregulation and neuroinflammation. While the current dopamine receptor-blocking antipsychotic drugs decrease psychotic symptoms and prevent relapse in the majority of patients with schizophrenia, there is a huge need to explore new treatment options that target other pathophysiological pathways. Read More

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http://dx.doi.org/10.3389/fpsyt.2018.00753DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365449PMC
January 2019

ClinTAD: a tool for copy number variant interpretation in the context of topologically associated domains.

J Hum Genet 2019 Feb 14. Epub 2019 Feb 14.

Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA, USA.

Standard clinical interpretation of DNA copy number variants (CNVs) identified by cytogenomic microarray involves examining protein-coding genes within the region and comparison to other CNVs. Emerging basic research suggests that CNVs can also exert a pathogenic effect through disruption of DNA structural elements such as topologically associated domains (TADs). To begin to integrate these discoveries with current practice, we developed ClinTAD, a free browser-based tool to assist with interpretation of CNVs in the context of TADs ( www. Read More

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http://dx.doi.org/10.1038/s10038-019-0573-9DOI Listing
February 2019