264,970 results match your criteria Current Protein & Peptide Science[Journal]


Protein corona variation in nanoparticles revisited: A dynamic grouping strategy.

Colloids Surf B Biointerfaces 2019 Apr 3;179:505-516. Epub 2019 Apr 3.

Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran; Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Bio-nano interface investigation models are mainly based on the type of proteins present on corona, bio-nano interaction responses and the evaluation of final outcomes. Due to the extensive diversity in correlative models for investigation of nanoparticles biological responses, a comprehensive model considering different aspects of bio-nano interface from nanoparticles properties to protein corona fingerprints appeared to be essential and cannot be ignored. In order to minimize divergence in studies in the era of bio-nano interface and protein corona with following therapeutic implications, a useful investigation model on the basis of RADAR concept is suggested. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2019.04.003DOI Listing

Nucleophosmin in leukemia: consequences of anchor loss.

Int J Biochem Cell Biol 2019 Apr 19. Epub 2019 Apr 19.

Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague 2, Czech Republic. Electronic address:

Nucleophosmin (NPM), one of the most abundant nucleolar proteins, has crucial functions in ribosome biogenesis, cell cycle control, and DNA-damage repair. In human cells, NPM occurs mainly in oligomers. It functions as a chaperone, undergoes numerous interactions and forms part of many protein complexes. Read More

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http://dx.doi.org/10.1016/j.biocel.2019.04.007DOI Listing

Functions of promyelocytic leukaemia zinc finger (Plzf) in male germline stem cell development and differentiation.

Reprod Fertil Dev 2019 Apr 23. Epub 2019 Apr 23.

Promyelocytic leukaemia zinc finger (Plzf), also known as zinc finger and BTB domain containing 16 (ZBTB16) or zinc-finger protein 145 (ZFP145), is a critical zinc finger protein of male germline stem cells (mGSCs). Multiple lines of evidence indicate that Plzf has a central role in the development, differentiation and maintenance of many stem cells, including mGSCs, and Plzf has been validated as an essential transcription factor for mammalian testis development and spermatogenesis. This review summarises current literature focusing on the significance of Plzf in maintaining and regulating self-renewal and differentiation of mGSCs, especially goat mGSCs. Read More

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http://dx.doi.org/10.1071/RD18252DOI Listing

The Role of Exopolysaccharides in Oral Biofilms.

J Dent Res 2019 Apr 22:22034519845001. Epub 2019 Apr 22.

1 Department of Oral Biology, Center for Oral Biology Research, Rutgers School of Dental Medicine, Newark, NJ, USA.

The oral cavity contains a rich consortium of exopolysaccharide-producing microbes. These extracellular polysaccharides comprise a major component of the oral biofilm. Together with extracellular proteins, DNA, and lipids, they form the biofilm matrix, which contributes to bacterial colonization, biofilm formation and maintenance, and pathogenesis. Read More

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http://dx.doi.org/10.1177/0022034519845001DOI Listing

ORAI1/STIM1 Interaction Intervenes in Stroke and in Neuroprotection Induced by Ischemic Preconditioning Through Store-Operated Calcium Entry.

Stroke 2019 May;50(5):1240-1249

IRCCS SDN, Naples, Italy (L.A.).

Background and Purpose- Disturbance of endoplasmic reticulum (ER) Ca homeostasis causes neuronal cell injury in stroke. By contrast, ischemic preconditioning (IPC)-a brief sublethal ischemic episode affording tolerance to a subsequent ischemic insult-restores ER Ca homeostasis. Under physiological conditions, ER calcium content is continuously refilled by the interaction between the ER-located Ca sensor STIM (stromal interacting molecule) 1 and the plasma membrane channel ORAI1 (a structural component of the CRAC calcium channel)-2 key mediators of the store-operated calcium entry (SOCE) mechanism. Read More

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http://dx.doi.org/10.1161/STROKEAHA.118.024115DOI Listing

Role of cyclin-dependent kinases (CDKs) in hepatocellular carcinoma: therapeutic potential of targeting CDKs signaling pathway.

Hepatol Res 2019 Apr 22. Epub 2019 Apr 22.

Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, China.

Hepatocellular carcinoma (HCC) is a common malignancy of the liver and is the second leading cause of cancer-related mortalities. Two-thirds of patients have advanced disease when diagnosed, and for these patients, treatment strategies remain limited. In addition, there is a high incidence of tumor recurrence after surgical resection with the current treatment regimens. Read More

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http://dx.doi.org/10.1111/hepr.13353DOI Listing

Novel Role of Macrophage Migration Inhibitory Factor in Upstream Control of the Unfolded Protein Response after Ethanol Feeding in Mice.

Alcohol Clin Exp Res 2019 Apr 22. Epub 2019 Apr 22.

Center for Liver Disease Research, Department of Inflammation and Immunity - Cleveland Clinic, Cleveland, OH, United States.

Introduction: Macrophage migration inhibitory factor (MIF), a pluripotent immune regulator, is an emerging mediator in Alcohol-related Liver Disease (ALD). MIF is associated with ALD progression through its chemokine- and cytokine-like activities.

Methods: Mechanistic studies into the role of MIF in ethanol-induced liver injury were performed in Mif mice and in C57BL/6J mice treated with a small molecule MIF antagonist, MIF098, after Gao-Binge (acute-on-chronic) ethanol feeding, an ethanol feeding protocol associated with hepatic neutrophilia and induction of the Unfolded Protein Response (UPR). Read More

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http://dx.doi.org/10.1111/acer.14065DOI Listing

Direct Electron Transfer of Enzymes Facilitated by Cytochromes.

Authors:
Su Ma Roland Ludwig

ChemElectroChem 2019 Feb 13;6(4):958-975. Epub 2018 Dec 13.

Biocatalysis and Biosensing Laboratory Department of Food Science and Technology BOKU - University of Natural Resources and Life Sciences Muthgasse 18 1190 Vienna Austria.

The direct electron transfer (DET) of enzymes has been utilized to develop biosensors and enzymatic biofuel cells on micro- and nanostructured electrodes. Whereas some enzymes exhibit direct electron transfer between their active-site cofactor and an electrode, other oxidoreductases depend on acquired cytochrome domains or cytochrome subunits as built-in redox mediators. The physiological function of these cytochromes is to transfer electrons between the active-site cofactor and a redox partner protein. Read More

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http://dx.doi.org/10.1002/celc.201801256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472588PMC
February 2019

Structural Insights for Anti-Influenza Vaccine Design.

Comput Struct Biotechnol J 2019 23;17:475-483. Epub 2019 Mar 23.

The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, China.

Influenza A virus are a persistent and significant threat to human health, and current vaccines do not provide sufficient protection due to antigenic drift, which allows influenza viruses to easily escape immune surveillance and antiviral drug activity. Influenza hemagglutinin (HA) is a glycoprotein needed for the entry of enveloped influenza viruses into host cells and is a potential target for anti-influenza humoral immune responses. In recent years, a number of broadly neutralizing antibodies (bnAbs) have been isolated, and their relative structural information obtained from the crystallization of influenza antigens in complex with bnAbs has provided some new insights into future influenza vaccine research. Read More

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http://dx.doi.org/10.1016/j.csbj.2019.03.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458449PMC

Pirarubicin reduces USP22 expression by inhibiting CREB-1 phosphorylation in HeLa cells.

Exp Ther Med 2019 May 27;17(5):4230-4236. Epub 2019 Mar 27.

Department of Pharmacology, College of Basic Medical Science, Jiujiang University, Jiujiang, Jiangxi 332000, P.R. China.

The expression of ubiquitin specific peptidase 22 (USP22) is upregulated in several types of cancer, and has been implicated in tumorigenesis. Pirarubicin (THP), an anthracycline antineoplastic drug, can induce apoptosis of several types of cancer cells. However, the molecular mechanisms underlying the action of THP remain to be elucidated. Read More

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http://www.spandidos-publications.com/10.3892/etm.2019.7447
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http://dx.doi.org/10.3892/etm.2019.7447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468931PMC
May 2019
1 Read

Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus.

Exp Ther Med 2019 May 26;17(5):4205-4212. Epub 2019 Mar 26.

Laboratory of Sport Physiology and Biomedicine, School of Physical Education and Sport Sciences, Fujian Normal University, Fuzhou, Fujian 350007, P.R. China.

Previous studies by our group have indicated that exercise intervention can ameliorate endothelial dysfunction, which is an early pathophysiological change of prediabetes mellitus. The present study aimed to test the hypothesis that nitric oxide synthases (NOSs), which are expressed in blood vessel endothelium, contribute to the mitigation of vascular endothelium-dependent dysfunction by aerobic exercise in prediabetes mellitus. A prediabetic rat model was established by feeding the rats an additional high-energy diet, and was confirmed by testing blood glucose levels, the area-under-the-curve for the blood glucose tests (P<0. Read More

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http://dx.doi.org/10.3892/etm.2019.7437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468937PMC

HS attenuates sepsis-induced cardiac dysfunction via a PI3K/Akt-dependent mechanism.

Exp Ther Med 2019 May 26;17(5):4064-4072. Epub 2019 Mar 26.

Department of Critical Care Medicine, The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang 832008, P.R. China.

The heart is the most vulnerable target organ in sepsis, and it has been previously reported that hydrogen sulfide (HS) has a protective role in heart dysfunction caused by sepsis. Additionally, studies have demonstrated that the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) signaling pathway has a protective function during sepsis. However, the potential association between HS and PI3K/Akt in sepsis-induced cardiac dysfunction is unclear. Read More

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http://www.spandidos-publications.com/10.3892/etm.2019.7440
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http://dx.doi.org/10.3892/etm.2019.7440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468938PMC
May 2019
1 Read

Dysregulated T Cell Activation and Aberrant Cytokine Expression Profile in Systemic Lupus Erythematosus.

Mediators Inflamm 2019 17;2019:8450947. Epub 2019 Mar 17.

Clinical Research Center, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China.

Accumulating evidence indicates a critical role for T cells and relevant cytokines in the pathogenesis of systemic lupus erythematosus (SLE). However, the specific contribution of T cells together with the related circulating cytokines in disease pathogenesis and organ involvement is still not clear. In the current study, we investigated relevant molecule expressions and cytokine levels in blood samples from 49 SLE patients and 22 healthy control subjects. Read More

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http://dx.doi.org/10.1155/2019/8450947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441516PMC

Genome-Wide Search for Tyrosine Phosphatases in the Human Genome Through Computational Approaches Leads to the Discovery of Few New Domain Architectures.

Evol Bioinform Online 2019 9;15:1176934319840289. Epub 2019 Apr 9.

National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India.

Reversible phosphorylation maintained by protein kinases and phosphatases is an integral part of intracellular signalling, and phosphorylation on tyrosine is extensively utilised in higher eukaryotes. Tyrosine phosphatases are enzymes that not only scavenge phosphotyrosine but are also involved in wide range of signalling pathways. As a result, mutations in these enzymes have been implicated in the pathogenesis of several diseases like cancer, autoimmune disorders, and muscle-related diseases. Read More

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http://dx.doi.org/10.1177/1176934319840289DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457024PMC

Synergistic efficacy of RLIP inhibition and 2'-hydroxyflavanone against DMBA-induced mammary carcinogenesis in SENCAR mice.

Mol Carcinog 2019 Apr 21. Epub 2019 Apr 21.

Department of Internal Medicine, Texas Tech University Health Sciences Center, Lubbock, Texas.

Substantial evidence suggests that 7,12-dimethylbenzanthracene (DMBA)-induced mammary carcinogenesis in mice mimics human breast cancer (BC) in many respects. Therefore, it has been used extensively to evaluate preventive and therapeutic agents for human BC. Mammary carcinogenesis induced by DMBA administration in female SENsitive to CARcinogen (SENCAR) mice was characterized by histopathological analysis of the mammary glands and alterations to the phosphatidylinositol 3-kinase/protein kinase B/cyclin-dependent kinase 1 (PI3K/Akt/CDK1) pathway. Read More

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http://dx.doi.org/10.1002/mc.23026DOI Listing
April 2019
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Bioactive peptides and proteins as alternative antiplatelet drugs.

Med Res Rev 2019 Apr 22. Epub 2019 Apr 22.

Department of Biotechnology, Alagappa University, Karaikudi, India.

Antiplatelet drugs reduce the risks associated with atherothrombotic events and show various applications in diverse cardiovascular diseases including myocardial infarctions. Efficacy of the current antiplatelet medicines including aspirin, clopidogrel, prasugrel and ticagrelor, and the glycoprotein IIb/IIIa antagonists, are limited due to their increased risks of bleeding, and antiplatelet drug resistance. Hence, it is important to develop new effective antiplatelet drugs, with fewer side-effects. Read More

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http://dx.doi.org/10.1002/med.21579DOI Listing

Curcumin induces apoptotic cell death and protective autophagy by inhibiting AKT/mTOR/p70S6K pathway in human ovarian cancer cells.

Arch Gynecol Obstet 2019 Apr 21. Epub 2019 Apr 21.

Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.

Purpose: Curcumin (Cur), a yellow-colored dietary flavor from the plant (Curcuma longa), has been demonstrated to potentially resist diverse diseases, including ovarian cancer, but drug resistance becomes a major limitation of its success clinically. The key molecule or mechanism associated with curcumin resistance in ovarian cancer still remains unclear. The aim of our study was to investigate the effects of curcumin on autophagy in ovarian cancer cells and elucidate the underlying mechanism. Read More

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http://link.springer.com/10.1007/s00404-019-05058-3
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http://dx.doi.org/10.1007/s00404-019-05058-3DOI Listing

Neurodegenerative Disease-Related Proteins within the Epidermal Layer of the Human Skin.

J Alzheimers Dis 2019 Apr 15. Epub 2019 Apr 15.

Department of Pharmacology & Therapeutics, Life Sciences Complex, McGill University, Montreal, Quebec, Canada.

There is increasing evidence suggesting that amyloidogenic proteins might form deposits in non-neuronal tissues in neurodegenerative disorders such as Alzheimer's or Parkinson's diseases. However, the detection of these aggregation-prone proteins within the human skin has been controversial. Using immunohistochemistry (IHC) and mass spectrometry tissue imaging (MALDI-MSI), fresh frozen human skin samples were analyzed for the expression and localization of neurodegenerative disease-related proteins. Read More

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http://dx.doi.org/10.3233/JAD-181191DOI Listing

Alkaloids from Piper nigrum synergistically enhanced the effect of paclitaxel against paclitaxel-resistant cervical cancer cells through the downregulation of Mcl-1.

J Agric Food Chem 2019 Apr 22. Epub 2019 Apr 22.

In the current study, nine amide alkaloids, including two new dimeric amides and a new natural product, were identified from Piper nigrum. Among them, seven compounds sensitized paclitaxel-resistant cervical cancer cells HeLa/PTX to paclitaxel. Piperine was a major component obtained from Piper nigrum, and its sensitization mechanism was investigated. Read More

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http://pubs.acs.org/doi/10.1021/acs.jafc.9b01320
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http://dx.doi.org/10.1021/acs.jafc.9b01320DOI Listing
April 2019
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M51R and Delta-M51 matrix protein of the vesicular stomatitis virus induce apoptosis in colorectal cancer cells.

Mol Biol Rep 2019 Apr 20. Epub 2019 Apr 20.

Cell and Molecular Research center, Golestan University of Medical Science, 1 Shastcola Ave, 5 km Sari Rd, Gorgan, Iran.

Colorectal cancer (CRC) is the third most common cancer in both men and women. Oncolytic viral-based therapy methods seem to be promising for CRC treatment. Vesicular stomatitis virus (VSV) is considered as a potent candidate in viral therapy for several tumors. Read More

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http://dx.doi.org/10.1007/s11033-019-04799-3DOI Listing

Effect of metformin on the size of the HIV reservoir in non-diabetic ART-treated individuals: single-arm non-randomised Lilac pilot study protocol.

BMJ Open 2019 Apr 20;9(4):e028444. Epub 2019 Apr 20.

The Ottawa Hospital, University of Ottawa, Ottawa, Ontario, Canada.

Introduction: People living with HIV (PLWH) on antiretroviral therapy (ART) do not progress to AIDS. However, they still suffer from an increased risk of inflammation-associated complications. HIV persists in long-lived CD4+ T cells, which form the major viral reservoir. Read More

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http://dx.doi.org/10.1136/bmjopen-2018-028444DOI Listing

24-Epibrassinolide alters DNA cytosine methylation of Linum usitatissimum L. under salinity stress.

Plant Physiol Biochem 2019 Apr 13;139:478-484. Epub 2019 Apr 13.

Institute of Biotechnology, Urmia University, Urmia, Iran; Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran.

Salinity is a common environmental challenge limiting worldwide agricultural crop yield. Plants employ epigenetic regulatory strategies, such as DNA methylation which relatively allows rapid adaptation to new conditions in response to environmental stresses. Brassinosteroids (BRs) are a novel group of phytohormones recognized as transcription and translation regulators which are able to mitigate the impact of environmental stresses on the plants. Read More

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http://dx.doi.org/10.1016/j.plaphy.2019.04.010DOI Listing
April 2019
3 Reads

Toward high-resolution in situ structural biology with cryo-electron tomography and subtomogram averaging.

Authors:
Florian Km Schur

Curr Opin Struct Biol 2019 Apr 18;58:1-9. Epub 2019 Apr 18.

Institute of Science and Technology Austria, Am Campus 1, A-3400 Klosterneuburg, Austria. Electronic address:

Cryo-electron tomography (cryo-ET) provides unprecedented insights into the molecular constituents of biological environments. In combination with an image processing method called subtomogram averaging (STA), detailed 3D structures of biological molecules can be obtained in large, irregular macromolecular assemblies or in situ, without the need for purification. The contextual meta-information these methods also provide, such as a protein's location within its native environment, can then be combined with functional data. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0959440X183017
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http://dx.doi.org/10.1016/j.sbi.2019.03.018DOI Listing
April 2019
1 Read

The CCL2 elevation in primary afferent fibers produces zymosan-induced hyperalgesia through microglia-mediated neuronal activation in the spinal dorsal horn.

Brain Res Bull 2019 Apr 18. Epub 2019 Apr 18.

Department of Pharmacology, Institute for Medical Science, Chonbuk National University Medical School, Jeonju, 54907, Republic of Korea. Electronic address:

Although C-C motif chemokine ligand 2 (CCL2) plays a critical role in the pathogenesis of neuropathic pain through neuron-microglia interactions, its pronociceptive function underlying inflammatory pain remains to be fully understood. The present study aimed to elucidate the potential role of CCL2 in pain sensitization following zymosan-induced inflammation. Intraplantar injection of zymosan into the rat hind paw significantly increased the expression of CCL2 in both dorsal root ganglions and the superficial dorsal horn. Read More

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http://dx.doi.org/10.1016/j.brainresbull.2019.04.014DOI Listing

sE-cadherin is upregulated in serum of patients with renal cell carcinoma and promotes tumor cell dissemination in vitro.

Urol Oncol 2019 Apr 18. Epub 2019 Apr 18.

University Hospital Frankfurt, Department of Urology, Frankfurt, Germany.

Introduction: Cadherin family proteins are involved in the tumorigenesis of several malignancies. However, their significance in renal cell carcinoma (RCC) has not been extensively investigated. The current study investigates the potential of several cadherins to perform as biomarkers for tumor detection and exert functional RCC activity. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10781439193009
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http://dx.doi.org/10.1016/j.urolonc.2019.03.001DOI Listing
April 2019
2 Reads

Safety Assessment of Immune-Mediated Adverse Reactions to Novel Food Proteins.

Trends Biotechnol 2019 Apr 17. Epub 2019 Apr 17.

Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM), C/ Nicolás Cabrera, 9, Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain.

Current international guidelines for the risk assessment of biotechnology-derived foods date back to 2003. We present new strategies and directions for assessing immune adverse reactions to novel food proteins. Understanding genetic factors involved in food allergy and the role of the gastrointestinal tract will streamline risk assessment strategies. Read More

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http://dx.doi.org/10.1016/j.tibtech.2019.03.010DOI Listing

Unsupervised machine learning in atomistic simulations, between predictions and understanding.

Authors:
Michele Ceriotti

J Chem Phys 2019 Apr;150(15):150901

Laboratory of Computational Science and Modeling, Institute des Materiaux, École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.

Automated analyses of the outcome of a simulation have been an important part of atomistic modeling since the early days, addressing the need of linking the behavior of individual atoms and the collective properties that are usually the final quantity of interest. Methods such as clustering and dimensionality reduction have been used to provide a simplified, coarse-grained representation of the structure and dynamics of complex systems from proteins to nanoparticles. In recent years, the rise of machine learning has led to an even more widespread use of these algorithms in atomistic modeling and to consider different classification and inference techniques as part of a coherent toolbox of data-driven approaches. Read More

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http://dx.doi.org/10.1063/1.5091842DOI Listing

The control of microbially induced corrosion by methyl eugenol - A dietary phytochemical with quorum sensing inhibitory potential.

Bioelectrochemistry 2019 Apr 11;128:186-192. Epub 2019 Apr 11.

Department of Biotechnology, Alagappa University, Karaikudi 630 003, Tamil Nadu, India. Electronic address:

The development of biofilms by microorganisms is conventionally attributed to microbially induced corrosion on stainless steel surfaces and leads to severe consequences in industrial and environmental settings. Since bacterial biofilm formation is regulated by the signal mediated quorum sensing (QS) system, targeting biofilms through QS inhibitors will possibly control biologically induced corrosion on the metal surface. In this study, biofilm formation on 316L stainless steel (SS 316L) immersed in a natural pond water system was effectively inhibited in the presence of the QS inhibitor methyl eugenol, as evidenced through epifluorescence microscopy, confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) analyses. Read More

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http://dx.doi.org/10.1016/j.bioelechem.2019.04.010DOI Listing

Morphological control of mitochondria as the novel mechanism of Gastrodia elata in attenuating mutant huntingtin-induced protein aggregations.

Phytomedicine 2018 Nov 14;59:152756. Epub 2018 Nov 14.

Department of Biotechnology and Animal Science, National Ilan University, Ilan, Taiwan, ROC. Electronic address:

Background: According to Compendium of Materia Medica, Gastrodia elata (GE) Blume as a top grade and frequently prescribed herbal medicine has been used in treating dizziness, headaches, and epilepsy, indicating a neuroprotective effect. Because GE is capable of suppressing a hyperactive liver and thus calming endogenous wind, and because Huntington's disease (HD) can be classified as a phenomenon of disturbed liver wind, it is suggested that GE might be beneficial in treating HD. However, although current studies support GE for the prevention of diverse neurodegenerations such as HD, its detailed mechanisms remain elusive. Read More

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http://dx.doi.org/10.1016/j.phymed.2018.11.016DOI Listing
November 2018

A Microparticle Approach for Non-viral Gene Delivery within 3D Human Mesenchymal Stromal Cell Aggregates.

Acta Biomater 2019 Apr 17. Epub 2019 Apr 17.

Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA; Materials Science Program, University of Wisconsin, Madison, WI 53706, USA; Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI 53705, USA; AO Foundation Collaborative Research Center, Davos, Switzerland. Electronic address:

Three-dimensional (3D) multicellular aggregates, in comparison to two-dimensional monolayer culture, can provide tissue culture models that better recapitulate the abundant cell-cell and cell-matrix interactions found in vivo. In addition, aggregates are potentially useful building blocks for tissue engineering. However, control over the interior aggregate microenvironment is challenging due to inherent barriers for diffusion of biological mediators (e. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.04.038DOI Listing

MICAL2 is expressed in cancer associated neo-angiogenic capillary endothelia and it is required for endothelial cell viability, motility and VEGF response.

Biochim Biophys Acta Mol Basis Dis 2019 Apr 17. Epub 2019 Apr 17.

Scuola Superiore Sant'Anna, Institute of Life Sciences, 56124 Pisa, Italy. Electronic address:

The capacity of inducing angiogenesis is a recognized hallmark of cancer cells. The cancer microenvironment, characterized by hypoxia and inflammatory signals, promotes proliferation, migration and activation of quiescent endothelial cells (EC) from surrounding vascular network. Current anti-angiogenic drugs present side effects, temporary efficacy, and issues of primary resistance, thereby calling for the identification of new therapeutic targets. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09254439193011
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http://dx.doi.org/10.1016/j.bbadis.2019.04.008DOI Listing
April 2019
1 Read

Prolactin Receptor Signaling Regulates a Pregnancy-Specific Transcriptional Program in Mouse Islets.

Endocrinology 2019 May;160(5):1150-1163

Division of Endocrinology, Department of Medicine, University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.

Pancreatic β-cells undergo profound hyperplasia during pregnancy to maintain maternal euglycemia. Failure to reprogram β-cells into a more replicative state has been found to underlie susceptibility to gestational diabetes mellitus (GDM). We recently identified a requirement for prolactin receptor (PRLR) signaling in the metabolic adaptations to pregnancy, where β-cell-specific PRLR knockout (βPRLRKO) mice exhibit a metabolic phenotype consistent with GDM. Read More

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http://dx.doi.org/10.1210/en.2018-00991DOI Listing

Role of Leptin in the Association Between Body Adiposity and Persistent Asthma: A Longitudinal Study.

Obesity (Silver Spring) 2019 Apr 20. Epub 2019 Apr 20.

VIMA: Aging and Chronic Diseases, Epidemiological and Public Health Approaches, INSERM U1168, Villejuif, France.

Objective: Obesity is a likely risk factor for asthma. However, underlying mechanisms by which obesity affects asthma activity remain poorly understood. This study aimed to investigate the role of leptin, an adipocyte-derived proinflammatory protein, as a mediator in the association between body adiposity (assessed using BMI, waist circumference, and body fat percentage) and persistent asthma. Read More

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http://dx.doi.org/10.1002/oby.22466DOI Listing
April 2019
1 Read

Current advances in haploid stem cells.

Protein Cell 2019 Apr 19. Epub 2019 Apr 19.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

Diploidy is the typical genomic mode in all mammals. Haploid stem cells are artificial cell lines experimentally derived in vitro in the form of different types of stem cells, which combine the characteristics of haploidy with a broad developmental potential and open the possibility to uncover biological mysteries at a genomic scale. To date, a multitude of haploid stem cell types from mouse, rat, monkey and humans have been derived, as more are in development. Read More

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http://dx.doi.org/10.1007/s13238-019-0625-0DOI Listing

The YB-1/EZH2/amphiregulin signaling axis mediates LPA-induced breast cancer cell invasion.

Arch Pharm Res 2019 Apr 19. Epub 2019 Apr 19.

Department of Pharmacology, College of Medicine, Konyang University, Myunggok Medical Building, 158 Gwanjeodong-ro, Seo-gu, Daejeon, 35365, Republic of Korea.

Lysophosphatidic acid (LPA) has been known to induce epithelial-mesenchymal transition (EMT) to stimulate cancer cell invasion, and resveratrol (3,5,4'-trans-trihydroxystilbene; REV) suppresses the invasion and metastasis of various cancers. The current study aimed to identify the underlying mechanism by which LPA aggravates breast cancer cell invasion and the reversal of this phenomenon. Immunoblotting and quantitative RT-PCR analysis revealed that LPA induces amphiregulin (AREG) expression. Read More

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http://link.springer.com/10.1007/s12272-019-01149-6
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http://dx.doi.org/10.1007/s12272-019-01149-6DOI Listing
April 2019
1 Read

Function of the cargo sorting dileucine motif in a cytomegalovirus immune evasion protein.

Med Microbiol Immunol 2019 Apr 19. Epub 2019 Apr 19.

Institute for Virology, University Medical Center of the Johannes Gutenberg-University Mainz and Research Center for Immunotherapy (FZI), Obere Zahlbacher Strasse 67, Hochhaus am Augustusplatz, 55131, Mainz, Germany.

As an immune evasion mechanism, cytomegaloviruses (CMVs) have evolved proteins that interfere with cell surface trafficking of MHC class-I (MHC-I) molecules to tone down recognition by antiviral CD8 T cells. This interference can affect the trafficking of recently peptide-loaded MHC-I from the endoplasmic reticulum to the cell surface, thus modulating the presentation of viral peptides, as well as the recycling of pre-existing cell surface MHC-I, resulting in reduction of the level of overall MHC-I cell surface expression. Murine cytomegalovirus (mCMV) was paradigmatic in that it led to the discovery of this immune evasion strategy of CMVs. Read More

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http://link.springer.com/10.1007/s00430-019-00604-x
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http://dx.doi.org/10.1007/s00430-019-00604-xDOI Listing
April 2019
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Potent and PPARα-independent anti-proliferative action of the hypolipidemic drug fenofibrate in VEGF-dependent angiosarcomas in vitro.

Sci Rep 2019 Apr 19;9(1):6316. Epub 2019 Apr 19.

Department of Pharmacology, Weill Cornell Medicine-Qatar, Education City, Doha, 24144, Qatar.

Angiosarcomas are highly aggressive tumors of endothelial origin, which carry a poor prognosis. Fenofibrate is a hypolipidemic drug, which acts by activating the transcription factor PPARα. It has also been widely reported to have 'anti-cancer' activity. Read More

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http://dx.doi.org/10.1038/s41598-019-42838-yDOI Listing
April 2019
5.078 Impact Factor

Epitope mapping of antibodies induced with a conserved rhinovirus protein generating protective anti-rhinovirus immunity.

Vaccine 2019 Apr 16. Epub 2019 Apr 16.

Cellular and Molecular Immunology Research Centre, School of Human Sciences, London Metropolitan University, London, UK; Airway Disease Section, National Heart and Lung Institute, Imperial College London, London, UK. Electronic address:

Human rhinovirus (RV) infections are the principle cause of common colds and precipitate asthma and chronic obstructive pulmonary disease (COPD) exacerbations. Currently there is no vaccine for RV which is largely due to the existence of ∼160 serotypes/strains. We demonstrated previously that immunising mice with highly conserved VP4 and VP2 regions of the RV polyprotein (RV-A16 VP0) generated cross-reactive immunity to RV in vivo. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0264410X193045
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http://dx.doi.org/10.1016/j.vaccine.2019.04.018DOI Listing
April 2019
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Combinatory microRNA serum signatures as classifiers of Parkinson's disease.

Parkinsonism Relat Disord 2019 Apr 11. Epub 2019 Apr 11.

Department of Biological Sciences, St. John's University, New York, NY, USA
. Electronic address:

Introduction: As current clinical diagnostic protocols for Parkinson's disease (PD) may be prone to inaccuracies there is a need to identify and validate molecular biomarkers, such as circulating microRNAs, which will complement current practices and increase diagnostic accuracy. This study identifies, verifies and validates combinatory serum microRNA signatures as diagnostic classifiers of PD across different patient cohorts.

Methods: 370 PD (drug naïve) and control serum samples from the Norwegian ParkWest study were used for identification and verification of differential microRNA levels in PD which were validated in a blind study using 64 NY Parkinsonism in UMeå (NYPUM) study serum samples and tested for specificity in 48 Dementia Study of Western Norway (DemWest) study Alzheimer's disease (AD) serum samples using miRNA-microarrays, and quantitative (q) RT-PCR. Read More

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http://dx.doi.org/10.1016/j.parkreldis.2019.04.010DOI Listing
April 2019
2 Reads

Outcomes of revision surgery after periprosthetic shoulder infection: a systematic review.

J Shoulder Elbow Surg 2019 Apr 16. Epub 2019 Apr 16.

Department of Orthopaedic and Trauma Surgery, "Magna Græcia" University, "Mater Domini" University Hospital, Catanzaro, Italy.

Background: Periprosthetic shoulder infection (PSI) is one of the most challenging complications after shoulder arthroplasty. Different treatments have been proposed, but the best surgical procedure remains disputed in the current literature. This systematic review investigated the outcomes of revision surgery after PSI. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10582746193012
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http://dx.doi.org/10.1016/j.jse.2019.02.014DOI Listing
April 2019
2 Reads

Function and Evolution of Upstream ORFs in Eukaryotes.

Trends Biochem Sci 2019 Apr 16. Epub 2019 Apr 16.

State Key Laboratory of Protein and Plant Gene Research, Center for Bioinformatics, School of Life Sciences, Peking University, Beijing, 100871, China. Electronic address:

There is growing interest in the role of translational regulation in cellular homeostasis during organismal development. Translation initiation is the rate-limiting step in mRNA translation and is central to translational regulation. Upstream open reading frames (uORFs) are regulatory elements that are prevalent in eukaryotic mRNAs. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09680004193005
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http://dx.doi.org/10.1016/j.tibs.2019.03.002DOI Listing
April 2019
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Effects of epigallocatechin-3-gallate on the HMGB1/RAGE pathway in PM-exposed asthmatic rats.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Department of Pediatrics, Children's Hospital Affiliated of Zhengzhou University, No.33 Long hu Wai huan East Road, Zhengzhou, 450018, Henan, China. Electronic address:

Current studies have shown that long-term exposure to fine particulate matter (PM) can aggravate lung injury in asthmatic children. The HMGB1/RAGE pathway may play an important role, but few studies on the HMGB1/RAGE signaling pathway in PM-induced asthma have been performed. Epigallocatechin-3-gallate (EGCG), which has antioxidant, anti-inflammatory and immunomodulatory effects, has not been examined in studies at home and abroad. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.03.165DOI Listing

The functional importance of structure in unstructured protein regions.

Authors:
Norman E Davey

Curr Opin Struct Biol 2019 Apr 16;56:155-163. Epub 2019 Apr 16.

Conway Institute of Biomolecular & Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland; Division of Cancer Biology, The Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK. Electronic address:

After two decades of research, intrinsically disordered regions (IDRs) are established as a widespread phenomenon. The growing understanding of the significant functional role of IDRs has challenged the structure-function paradigm, proving irrefutably that a stably folded structure is not a strict requirement for function. Nonetheless, (un)structure-function relationships remain at the core of IDR-mediated interactions. Read More

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http://dx.doi.org/10.1016/j.sbi.2019.03.009DOI Listing

Honokiol for cancer therapeutics: A traditional medicine that can modulate multiple oncogenic targets.

Pharmacol Res 2019 Apr 16. Epub 2019 Apr 16.

Cancer Biology Laboratory and DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB) Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, 781039, India. Electronic address:

In spite of billions of dollars expended on cancer research every year, the incidence rate and the mortality rate due to this widespread disease has increased drastically over the last few decades. Recent reports from the World Health Organization advocate that overall global cancer burden and deaths due to cancer are expected to double by the next decade. Synthetic drugs developed as chemotherapeutics have repeatedly shown adverse side effects and development of chemoresistance. Read More

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http://dx.doi.org/10.1016/j.phrs.2019.04.004DOI Listing
April 2019
2 Reads

Frailty biomarkers in humans and rodents: current approaches and future advances.

Mech Ageing Dev 2019 Apr 16. Epub 2019 Apr 16.

Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA; Department of Pharmacology, The University of New South Wales, Sydney, Australia. Electronic address:

Even though they would have great benefit across research and clinical fields, currently there are no accepted biomarkers of frailty. Cross-sectional studies in humans have identified promising candidates including inflammatory markers such as IL-6, immune markers such as WBC count, clinical markers such as albumin, endocrine markers such as vitamin D, oxidative stress markers such as isoprostanes, proteins such as BDNF and epigenetic markers such as DNA methylation, but there are limitations to the current state of the research. Future approaches to the identification of frailty biomarkers should include longitudinal studies, studies using animal models of frailty, studies incorporating novel biomarkers combined into composite panels, and studies investigating sex differences and potential overlap between markers of biological age and frailty. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00476374193003
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http://dx.doi.org/10.1016/j.mad.2019.03.007DOI Listing
April 2019
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Molecular impacts of photobiomodulation on bone regeneration: A systematic review.

Prog Biophys Mol Biol 2019 Apr 16. Epub 2019 Apr 16.

School of Dentistry, The University of Queensland, Herston, QLD, 4006, Australia. Electronic address:

Photobiomodulation (PBM) encompasses a light application aimed to increase healing process, tissue regeneration, and reducing inflammation and pain. PBM is specifically aimed to modify the expression of cellular molecules; however, PBM impacts on cellular and molecular pathways especially in bone regenerative medicine have been investigated in scattered different studies. The purpose of the current study is to systematically review evidence on molecular impact of PBM on bone regeneration. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00796107193001
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http://dx.doi.org/10.1016/j.pbiomolbio.2019.04.005DOI Listing
April 2019
3 Reads
2.274 Impact Factor

Two-component cross-linkable gels for fabrication of solid oral dosage forms.

J Control Release 2019 Apr 16. Epub 2019 Apr 16.

Faculty of Biotechnology and Food Engineering, Technion - Israel Institute of Technology, Haifa 3200003, Israel; Russell Berrie Nanotechnology Institute, Technion - Israel Institute of Technology, Haifa 3200003, Israel.

Current three-dimensional (3D) printing techniques involve the solidification of the injected materials by means of UV irradiation, evaporation of organic solvents, or harsh heating and cooling processes. These methods limit the printing of many sensitive bio-active molecules such as proteins. We describe a novel 3D printing technique based on two complementary liquid copolymers, PEG-PCL-SC and PEG-PCL-NH, that are injected in a coordinated fashion and react with each other to form a pre-designed 3D pill. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01683659193022
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http://dx.doi.org/10.1016/j.jconrel.2019.04.021DOI Listing
April 2019
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Morin post-treatment confers neuroprotection in a novel rat model of mild repetitive traumatic brain injury by targeting dementia markers, APOE, autophagy and Wnt/β-catenin signaling pathway.

Brain Res 2019 Apr 16. Epub 2019 Apr 16.

Department of Pharmacology & Toxicology, Cairo University, Cairo, Egypt. Electronic address:

Exposure to repetitive brain trauma has gained attention for its similarity to sport-related trauma. The traumatic brain injury (TBI) is strongly associated with neurodegenerative pathology that affects cognition, memory and behavior. The current study developed a novel mild repetitive traumatic brain injury (mRTBI) model to highlight some of the possible molecular pathological mechanisms compared to those of single trauma. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00068993193018
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http://dx.doi.org/10.1016/j.brainres.2019.04.003DOI Listing
April 2019
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Arrdc2 and Arrdc3 Elicit Divergent Changes in Gene Expression in Skeletal Muscle following Anabolic and Catabolic Stimuli.

Physiol Genomics 2019 Apr 19. Epub 2019 Apr 19.

Rancho BioSciences, United States.

Skeletal muscle is a highly plastic organ regulating various processes in the body. As such, loss of skeletal muscle underlies the increased morbidity and mortality risk that is associated with numerous conditions. However, no therapies are available to combat the loss of muscle mass during atrophic conditions, which is due in part to the incomplete understanding of the molecular networks altered by anabolic and catabolic stimuli. Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00007
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http://dx.doi.org/10.1152/physiolgenomics.00007.2019DOI Listing
April 2019
2 Reads

Promising therapies for the treatment of frontotemporal dementia clinical phenotypes: from symptomatic to disease-modifying drugs.

Expert Opin Pharmacother 2019 Apr 19:1-17. Epub 2019 Apr 19.

a Neurodegenerative Disease Unit, Department of Basic Medical Sciences, Neuroscience and Sense Organs , University of Bari "Aldo Moro" , Bari , Italy.

Introduction: Frontotemporal dementia (FTD) is a heterogeneous clinical entity that includes several disorders characterized by different cellular mechanisms. Distinctive clinical features in FTD include behavioral, affective, and cognitive symptoms. Unfortunately, little progress has been made over the past 20 years in terms of the development of effective disease-modifying drugs with the currently available symptomatic treatments having limited clinical utility. Read More

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http://dx.doi.org/10.1080/14656566.2019.1598377DOI Listing