1,478 results match your criteria Current Molecular Medicine[Journal]


Sumoylation Plays Fundamental Roles During Eye Development and Pathogenesis.

Curr Mol Med 2018 ;18(8):507-508

One-Hundred Talent Professor of Ophthalmology Section Director, Eye Development & Molecular Pathogenesis The State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University, China.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/156652401808190130160225DOI Listing
January 2018

Potential Mutations in Chinese Pathologic Myopic Patients and Contributions to Phenotype.

Curr Mol Med 2019 Feb 11. Epub 2019 Feb 11.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou. China.

Purpose: Pathologic myopia is a leading cause of visual impairment in East Asia. The aim of this study was to investigate the potential mutations in Chinese pathologic myopic patients and to analyze the correlations between genotype and clinical phenotype.

Methods: One hundred and three patients with pathologic myopia and one hundred and nine unrelated healthy controls were recruited from Zhongshan Ophthalmic Center. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190211120016DOI Listing
February 2019

Estriol inhibits dermcidin isoform-2 induced inflammatory cytokine expression via nitric oxide synthesis in human neutrophil.

Curr Mol Med 2019 Feb 7. Epub 2019 Feb 7.

Post Graduate Department of Biochemistry, Cell & Molecular Therapeutics Laboratory Oriental Institute of Science and Technology, Rangamati, Midnapore, WB, 721102. India.

Background: An increase in the level of cytokines like TNF-α and IL-6 causes the inflammatory surge in acute ischemic heart disease (AIHD).

Objective: A high-level dermcidin isoform-2 (DCN-2) occurrence in AIHD was subjected to determine a possible regulation on cytokines expression.Effect of estrogen to counteract the inflammatory response was determined. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190208095754DOI Listing
February 2019
1 Read

Histone Deacetylase Inhibition Attenuates Cardiomyocyte Hypoxia-Reoxygenation Injury.

Curr Mol Med 2019 Feb 7. Epub 2019 Feb 7.

Department of Surgery, University of Michigan Health System, Ann Arbor, Michigan. United States.

Cardiac reperfusion injury can have devastating consequences. Histone deacetylase (HDAC) inhibitors are potent cytoprotective agents, but their role in the prevention of cardiac injury remains ill-defined. We sought to determine the therapeutic potential of HDAC inhibitors in an in vitro model of cardiomyocyte hypoxia-reoxygenation (H/R). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190208102729DOI Listing
February 2019

Naoxintong Retards Atherosclerosis by Inhibiting Foam Cell Formation through Activating PPARα Pathway.

Curr Mol Med 2019 Feb 7. Epub 2019 Feb 7.

Department of Cardiology, Zhongshan Hospital, Fudan University. Shanghai Institute of Cardiovascular Diseases, Shanghai 200032. China.

Backgrounds: We recently reported that Naoxintong (NXT), a China Food and Drug Administration (FDA)-approved cardiac medicine, could reduce the plaque size, but the underlying mechanism remains elusive now.

Objective: In this study, we investigated the effects of NXT on foam cell accumulation both in vivo and in vitro and explored related mechanisms.

Method: THP-1 cells and bone marrow derived macrophages were incubated with oxidized low density lipoprotein (ox-LDL) with/without Naoxintong. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190207143207DOI Listing
February 2019

Human disease modelling techniques: current progress.

Curr Mol Med 2019 Feb 6. Epub 2019 Feb 6.

Institute of Gene Biology, Russian Academy of Sciences, Moscow. Russian Federation.

Advances in genetic engineering and genomic studies facilitated development of animal models of human diseases. To date, numerous models based on different animal species are available for the most socially significant human diseases, such as cardiovascular disorders, cancer, neurodegenerative and metabolic disorders. Modern genetic methods allow creating animals with certain genes up- or down-regulated, as well as bearing specific mutations. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190206204357DOI Listing
February 2019

GDF11 attenuated ANG II-induced hypertrophic cardiomyopathy and expression of ANP, BNP and beta-MHC through down-regulating CCL11 in mice.

Curr Mol Med 2019 Feb 3. Epub 2019 Feb 3.

Department of Cardiology, Gong Li Hospital, 219 Miao Pu Road, Pu Dong New Area, Shanghai 200135. China.

Background: Growth differentiation factor 11 (GDF11) decreases with age, and increased C-C motif chemokine 11 (CCL11) is involved in aging. However, it is unclear about effects of GDF11 on Angiotensin II (ANG II)-induced hypertrophic cardiomyopathy and expression of markers for volume overload and hypertrophy such as ANP, BNP and beta-MHC, as well as the relationship between GDF11 and CCL11 in hypertrophhic cardiomyopathy. Therefore, the current study aimed to examine effects of GDF11 on ANG II-induced hypertrophic cardiomyopathy and expression of ANP, BNP and beta-MHC in mice, and explore possible molecular mechanisms. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190204112753DOI Listing
February 2019
2 Reads

New approach for cancer immunotherapy based on the cancer stem cell antigens properties.

Curr Mol Med 2019 Feb 3. Epub 2019 Feb 3.

Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz. Iran.

Background: Cancer stem cells (CSCs) are a rare population of tumor cells, which play an important role in tumor initiation, progression, and maintenance. The concept that cancer cells arise from stem cells was presented about 150 years ago. Nowadays, because of the heterogeneity of tumor cells, this hypothesis has been renewed. Read More

View Article

Download full-text PDF

Source
http://www.eurekaselect.com/169602/article
Publisher Site
http://dx.doi.org/10.2174/1566524019666190204114721DOI Listing
February 2019
3 Reads

Th1/Th17 cytokine profile is induced by macrophage migration inhibitory factor in peripheral blood mononuclear cells from rheumatoid arthritis patients.

Curr Mol Med 2019 Jan 29. Epub 2019 Jan 29.

Instituto de Investigacion en Ciencias Biomedicas, CUCS, Universidad de Guadalajara, Guadalajara, Jalisco. Mexico.

Background: Macrophage migration inhibitory factor (MIF) is an immunoregulatory cytokine that plays a crucial role as regulator of the innate and adaptive immune responses and takes part in the destructive process of the joint in rheumatoid arthritis (RA) by promoting angiogenesis and inducing proinflammatory cytokines and matrix metalloproteinases (MMP). We evaluated if recombinant human MIF (rhMIF) induces the production of TNF-α, IFN-γ, IL-1β, IL-6, IL-10, IL-17A, and IL-17F in peripheral blood mononuclear cells (PBMC) from RA patients and control subjects (CS).

Methods: The PBMC from RA patients and CS were stimulated for 24 hours with combinations of LPS, rhMIF or the MIF antagonist ISO-1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190129123240DOI Listing
January 2019
1 Read

Small Incision Femtosecond Laser-assisted X-ray-irradiated Corneal Intrastromal Xenotransplantation in Rhesus Monkeys: A preliminary study.

Curr Mol Med 2019 Jan 29. Epub 2019 Jan 29.

Zhongshan Ophthalmic Center and State Key Laboratory of Ophthalmology, SunYat-sen University. Guangzhou 510060. China.

Background: Gamma-ray irradiation could significantly induce widespread apoptosis in corneas and reduced the allogenicity of donor cornea. And the X-rays may have the similar biological effects. The feasibility and effects of X-ray-irradiated corneal lamellae has not been assessed yet. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190129123935DOI Listing
January 2019

The Bromodomain and Extra-Terminal Protein Inhibitor OTX015 Suppresses T Helper Cell Proliferation and Differentiation.

Curr Mol Med 2019 Jan 25. Epub 2019 Jan 25.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, 510060. China.

Background: Dynamic epigenetic alterations accompanying CD4+ T helper cell differentiation have been implicated in multiple autoimmune diseases. The bromodomain and extra-terminal (BET) proteins are epigenetic regulators that recognize and bind to acetylated histones in chromatin and are targets for pharmacological inhibition. In this study we tested a new BET inhibitor under clinical development, OTX015, to interrogate its effects on key CD4+ T cell subsets associated with autoimmunity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190126112238DOI Listing
January 2019
1 Read

Localization Patterns of Sumoylation Enzymes E1, E2 and E3 in Ocular Cell Lines Predict Their Functional Importance.

Curr Mol Med 2018 ;18(8):516-522

The State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, #7 Jinsui Road, Guangzhou, Guangdong 510230, China.

Purpose: It is well established now that protein sumoylation acts as an important regulatory mechanism mediating control of ocular development through regulation of multiple transcription factors. Yet the functional mechanisms of each factor modulated remain to be further explored using the available in vitro systems. In this regard, various ocular cell lines including HLE, FHL124, αTN4-1, N/N1003A and ARPE-19 have been demonstrated to be useful for biochemical and molecular analyses of normal physiology and pathogenesis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190112144436DOI Listing
January 2018
1 Read

Analysis of the Differential Expression Patterns of Sumoylation Enzymes E1, E2 and E3 in Ocular Cell Lines.

Curr Mol Med 2018 ;18(8):509-515

The State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, #7 Jinsui Road, Guangzhou, Guangdong 510230, China.

Purpose: Protein sumoylation is a well established regulatory mechanism to control many cellular processes such as chromatin structure dynamics, transcriptional regulation of gene expression, cell proliferation and differentiation, cell transformation and carcinogenesis, autophagy and senescence. In the vertebrate vision system, we and others have revealed that sumoylation plays important roles in regulating differentiation of several ocular tissues during eye development. To further elucidate the functional mechanisms of sumoylation, in vitro assay systems are needed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190112143636DOI Listing
January 2018
1 Read

Localization Analysis of Seven De-sumoylation Enzymes (SENPs) in Ocular Cell Lines.

Curr Mol Med 2018 ;18(8):523-532

The State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, #7 Jinsui Road, Guangzhou, Guangdong 510230, China.

Purpose: It is now well established that protein sumoylation acts as an important regulatory mechanism modulating functions over three thousand proteins. In the vision system, protein conjugation with SUMO peptides can regulate differentiation of multiple ocular tissues. Such regulation is often explored through analysis of biochemical and physiological changes with various cell lines in vitro. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190112142025DOI Listing
January 2018
4 Reads

Comparative Analysis of the Interphotoreceptor Retinoid Binding Protein Induced Models of Experimental Autoimmune Uveitis in B10.RIII versus C57BL/6 Mice.

Curr Mol Med 2019 Jan 12. Epub 2019 Jan 12.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University (SYSU), Guangzhou, 510060 . China.

Experimental autoimmune uveitis (EAU) represents autoimmune uveitis in human, among which B10.RIII and C57BL/6 are the frequently used strains in mice, but to day no study has been reported to compare EAU disease between the two strains. Here we compared the differences in morphology, pathology, visual function of the retinal inflammation and Th1/Th17 immune responses in the EAU models induced by the interphotoreceptor retinoid binding protein (IRBP) between the B10. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190112132610DOI Listing
January 2019
1 Read

Differential Expression of Seven De-sumoylation Enzymes (SENPs) in Major Ocular Tissues of Mouse Eye.

Curr Mol Med 2018 ;18(8):533-541

The State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, #7 Jinsui Road, Guangzhou, Guangdong 510230, China.

Purpose: Protein Sumoylation is one of the most important and prevalent posttranscriptional modification. Increasing evidence have shown that the SENPs (sentrin/SUMOspecific proteases) are critical for steady-state levels of SUMO modification of target proteins, and protein de-sumoylation modulates a great diversity of biological processes including transcription, development, differentiation, neuroprotection, as well as pathogenesis. In the vertebrate eye, we and others have previously shown that sumoylation participated in the differentiation of major ocular tissues including retina and lens. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190112132103DOI Listing
January 2018
5 Reads

Sodium Iodate-Induced Mouse Model of Age-Related Macular Degeneration Displayed Altered Expression Patterns of Sumoylation Enzymes E1, E2 and E3.

Curr Mol Med 2018 ;18(8):550-555

The State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, #7 Jinsui Road, Guangzhou, Guangdong 510230, China.

Purpose: Protein sumoylation is a highly dynamic and reversible post-translational modification, involving covalently conjugation of the small ubiquitin-like modifier (SUMO) to the lysine residue of the target protein. Similar to ubiquitination, sumoylation is catalyzed by E1, E2 and several E3 ligases. However, sumoylation usually does not cause protein degradation but alter the target function through diverse mechanisms. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190112101147DOI Listing
January 2018
5 Reads

The Sumoylation Modulated Tumor Suppressor p53 Regulates Cell Cycle Checking Genes to Mediate Lens Differentiation.

Curr Mol Med 2018 ;18(8):556-565

The State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, #7 Jinsui Road, Guangzhou, Guangdong 510230, China.

Purpose: The tumor suppressor p53 is a master regulator of apoptosis and also plays a key role in cell cycle checking. In our previous studies, we demonstrated that p53 directly regulates Bak in mouse JB6 cells and that p53-Bak signaling axis plays an important role in mediating EGCG-induced apoptosis. Furthermore, we have recently demonstrated that the same p53-Bak apoptotic signaling axis executes an essential role in regulating lens cell differentiation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190111154450DOI Listing
January 2018
6 Reads

Analysis of Non-Sumoylated and Sumoylated Isoforms of Pax-6, the Master Regulator for Eye and Brain Development in Ocular Cell Lines.

Curr Mol Med 2018 ;18(8):566-573

The State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, #7 Jinsui Road, Guangzhou, Guangdong 510230, China.

Purpose: Pax-6 is a master regulator for eye and brain development. Previous studies including ours have shown that Pax-6 exists in 4 major isoforms. According to their sizes, they are named p48, p46, p43 and p32 with the corresponding molecular weight of 48, 46, 43 and 32 kd, respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190111153310DOI Listing
January 2018
6 Reads

Altered Expression Patterns of the Sumoylation Enzymes E1, E2 and E3 Are Associated with Glucose Oxidase- and UVA-Induced Cataractogenesis.

Curr Mol Med 2018 ;18(8):542-549

The State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, #7 Jinsui Road, Guangzhou, Guangdong 510230, China.

Purpose: Protein sumoylation is a well established regulatory mechanism that regulates chromatin structure and dynamics, cell proliferation and differentiation, stress response and cell apoptosis. In the vertebrate eye, we and others have shown that sumoylation plays an indispensable role in regulating eye development. During stress induction and aging process, the ocular tissues gradually loss their normality and develop major ocular diseases such as cataract and aging-related macular degeneration. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190111152324DOI Listing
January 2018
3 Reads

A Traditional Chinese Patent Medicine ZQMT for Neovascular Age-Related Macular Degeneration: a multicenter randomized clinical trial.

Curr Mol Med 2019 Jan 7. Epub 2019 Jan 7.

Department of Ophthalmology, Peking Union Medical College, Beijing 100730. China.

Background: Anti-VEGF agent ranibizumab has been extensively used as a standard treatment for wet AMD. We investigated whether traditional Chinese medicine could serve as a complimentary therapy for this disease.

Methods: 144 patients with neovascular age-related macular degeneration received either intravitreal ranibizumab treatment as needed plus placebo or intravitreal ranibizumab treatment as needed plus a Chinese FDA approved traditional Chinese patent medicine named ZQMT. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190107155311DOI Listing
January 2019
11 Reads

Inhibition of sumoylation alleviates oxidative stress-induced retinal pigment epithelial cell senescence and represses proinflammatory gene expression.

Curr Mol Med 2019 Jan 7. Epub 2019 Jan 7.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060. China.

Advanced age is the largest risk factor for age-related macular degeneration (AMD). Sumoylation is a reversible post-translational modification that conjugates small peptide, small ubiquitin-like modifier (SUMO), to a target protein. Dysregulation of sumoylation is recently found to be critically involved in several age-related disorders. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190107154250DOI Listing
January 2019
1 Read

Determination of Expression Patterns of Seven De-sumoylation Enzymes in Major Ocular Cell Lines.

Curr Mol Med 2019 Jan 7. Epub 2019 Jan 7.

The State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, #7 Jinsui Road, Guangzhou, Guangdong 510230. China.

Accumulated evidence have well established that protein sumoylation plays multiple roles in various cellular processes. In the vertebrate eye, we and others have demonstrated that sumoylation displays indispensable roles in regulating eye development. Various ocular cell lines including human embryonic cell line (FHL124), the SV40-large T-transformed human lens epithelial cell line (HLE), the SV40-large T-transformed mouse lens epithelial cell line (αTN4-1), the rabbit lens epithelial cell line (N/N1003A) and the human retina pigment epithelial cell line (ARPE-19) have been extensively used for studying various cellular functions and disease processes including sumoylation functions, and mechanisms for cataract and age-related macular degeneration (AMD). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190107153440DOI Listing
January 2019
5 Reads

The Status of Biochemical and Molecular Markers of Oxidative Stress in Preeclamptic Saudi Patients.

Curr Mol Med 2018 ;18(7):475-485

Chair of Medical and Molecular Genetics Research, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.

Purpose: In the light of contradictory results and paucity of information, this comprehensive study examines the activities and levels of key antioxidants and oxidants/pro-oxidants in preeclamptic patients.

Methods: Antioxidants including glutathione peroxidase, glutathione reductase, glutathione-S-transferase, superoxide dismutase, catalase, reduced glutathione, selenium, zinc, copper and manganese, as well as marker oxidants/pro-oxidants including hydrogen peroxide, superoxide anions, malondialdehyde, protein carbonyls and oxidized glutathione were determined in plasma and placental tissues of nonpregnant, healthy pregnant and preeclamptic subjects.

Results: Data indicated that all plasma antioxidants underwent moderate but significant decreases (p< 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190104105843DOI Listing
January 2018
1 Read

All Oral Interferon-free Direct-acting Antivirals as Combination Therapies to Cure Hepatitis C.

Curr Mol Med 2018 ;18(7):409-435

Pharmaceutical Chemistry Department, College of Pharmacy, Umm Al Qura University, Al-Abidiyah, Makkah, P.O Box. 13578, Postal code 21955, Saudi Arabia.

Chronic hepatitis C (CHC) virus infection and associated hepatic diseases are still challenging, and the disease burden remains significant around the world. Overall treatment rates for the chronically infected patients have been "dismally poor" and that treatment completion of dual-therapy- pegylated interferon (PEG-IFN) and ribavirin (RBV) is suboptimal in the real-world clinical settings. The approval of first, second and next-generation direct-acting antivirals (DAAs) represents a major breakthrough in hepatitis C virus (HCV) therapeutics to treat CHC infected individuals. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190104110439DOI Listing
January 2018
8 Reads

Phytochemicals as uropathognic Escherichia coli FimH antagonist: In vitro and in silico approach.

Curr Mol Med 2019 Jan 3. Epub 2019 Jan 3.

Department of Biochemistry, Patna University, Patna, 800005, Bihar. India.

Background: Urinary tract infection (UTI) is caused by uropathogenic Escherichia coli (UPEC). The UPEC initiate pathogenesis by expressing type 1 pili, which attach to membrane receptors on the uroepithelial cells. Inhibition of attachment can provide a valuable target for prophylaxis in symptom free milieu. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666190104104507DOI Listing
January 2019
9 Reads
3.621 Impact Factor

Potential therapeutic applications of MDA-9/Syntenin-NF-κB-RKIP loop in human liver carcinoma.

Curr Mol Med 2019 Jan 3. Epub 2019 Jan 3.

Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, School of Science, University of Palermo. Italy.

Background: Overexpression of MDA-9/Syntenin occurs in multiple human cancer cell lines and is associated with higher grade of tumor classification, invasiveness and metastasis. In some cases, its role in cancer biology depends on relationships between MDA-9/Syntenin and NF-κB.

Objective: This study aims to analyze the presence of a regulation loop like that between MDA-9/Syntenin - NF-κB - RKIP in human liver carcinoma. Read More

View Article

Download full-text PDF

Source
http://www.eurekaselect.com/168753/article
Publisher Site
http://dx.doi.org/10.2174/1566524019666190104105043DOI Listing
January 2019
5 Reads

HSP70 Inhibitors Reduce The Osteoblast Migration By Epidermal Growth Factor.

Curr Mol Med 2018 ;18(7):486-495

Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Japan.

Background: We have recently reported that epidermal growth factor (EGF) induces migration of osteoblast-like MC3T3-E1 cells through the activation of p44/p42 mitogen-activated protein (MAP) kinase, p38 MAP kinase, stress-activated protein kinase/ c-Jun N-terminal kinase (SAPK/JNK) and Akt. Furthermore, we demonstrated that heat shock protein 70 (HSP70) down-regulates the transforming growth factor-β- stimulated vascular endothelial growth factor synthesis via suppression of p38 MAP kinase in osteoblast-like MC3T3-E1 cells. However, the exact role of HSP70 underlying osteoblast migration is not fully elucidated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666181213112847DOI Listing
January 2018
1 Read

Madhuca indica Inhibits Breast Cancer Cell Proliferation by Modulating COX-2 Expression.

Curr Mol Med 2018 ;18(7):459-474

Department of Signal Transduction and Biogenic Amines, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata-700026, India.

Background: Madhuca indica belongs to the family sapotaceae, commonly known as Mahua. It is primarily known for alcoholic beverage production and is reported to have anti-inflammatory, analgesic and antipyretic properties. Madhuca indica has also been reported to be effective in several diseases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666181212100808DOI Listing
January 2018
3 Reads

Loss of the Nodal modulator Nomo results in chondrodysplasia in zebrafish.

Curr Mol Med 2018 ;18(7):448-458

The Center for Heart Development, State Key Lab of Development Biology of Freshwater Fish, The National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, Changsha, Hunan, China.

Background: Transforming growth factor-β (TGF-β)/nodal signaling is involved in early embryonic patterning in vertebrates. Nodal modulator (Nomo, also called pM5) is a negative regulator of nodal signaling. Currently, the role of nomo gene in cartilage development in vertebrates remains unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666181212095307DOI Listing
January 2018
3 Reads

Generation of a Primary Hyperoxaluria Type 1 Disease Model Via CRISPR/Cas9 System in Rats.

Curr Mol Med 2018 ;18(7):436-447

Department of Pediatric Urology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Primary hyperoxaluria type 1 (PH1) is an inherited disease caused by mutations in alanine-glyoxylate aminotransferase (AGXT). It is characterized by abnormal metabolism of glyoxylic acid in the liver leading to endogenous oxalate overproduction and deposition of oxalate in multiple organs, mainly the kidney. Patients of PH1 often suffer from recurrent urinary tract stones, and finally renal failure. Read More

View Article

Download full-text PDF

Source
http://www.eurekaselect.com/168285/article
Publisher Site
http://dx.doi.org/10.2174/1566524019666181212092440DOI Listing
January 2018
15 Reads

Cystathionine β-synthase Induces Multidrug Resistance and Metastasis in Hepatocellular Carcinoma.

Curr Mol Med 2018 ;18(7):496-506

College of Pharmacy, Joint International Research Laboratory of Food & Medicine,Henan University, Kaifeng 475004, Henan Province, China.

Objective: This study aims to analyze whether Cystathionine β-synthase (CBS) plays roles in hepatocellular carcinoma (HCC) drug resistance.

Methods: MTS assay was used to detect the effect of chemotherapeutic drugs doxorubicin (DOX) and sunitinib on HCC cell viability and cell growth. Intracellular doxorubicin accumulation assay was performed to evaluate the sensitivity of DOX and sunitinib in HCC cells and the function of multidrug resistance-associated protein Pglycoprotein (P-gp). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524019666181211162754DOI Listing
January 2018
3 Reads

Differential Expression of Sumoylation Enzymes SAE1, U BA2, UBC9, PIAS1 and RanBP2 in Major Ocular Tissues of Mouse Eye.

Curr Mol Med 2018 ;18(6):376-382

Key Laboratory of Protein Chemistry and Developmental Biology, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.

It is now well established that protein sumoylation is an important mechanism to regulate multiple cellular processes including gene transcription, chromatin structure, cell proliferation and differentiation, as well as pathogenesis. In the vertebrate eye, we and others have previously shown that sumoylation can regulate differentiation of major ocular tissues including retina and lens. However, the expression patterns of the three types of sumoylation enzymes, the activating enzymes SAE1 and UBA2, the conjugating enzyme UBC9, and the ligating enzymes such as RanBP2 and PIAS1 have not been well studied in the ocular tissues. Read More

View Article

Download full-text PDF

Source
http://www.eurekaselect.com/167434/article
Publisher Site
http://dx.doi.org/10.2174/1566524018666181116112208DOI Listing
January 2018
9 Reads

Digitoxin Suppresses Store Operated Calcium Entry by Modulating Phosphorylation and the Pore Region of Orai1.

Curr Mol Med 2018 ;18(6):392-399

Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China.

Background: Store-operated calcium entry (SOCE), primarily mediated by Orai1 and stromal interaction molecule 1 (STIM1), is a major Ca2+ influx pathway that has been linked to human diseases including myopathy, epilepsy, immunodeficiency, and cancer. Despite of the recent rapid progress of dissecting molecular mechanisms underlying SOCE activation, the development of therapies against dysfunctional SOCE significantly lags behind, partly due to the lack of more specific pharmacological tools and poor understanding of currently available SOCE modifiers, including the a newly identified SOCE inhibitor, digitoxin.

Objective And Methods: Capitalizing on Ca2+ imaging and pharmacological tools, we aimed to systemically delineate the mechanism of action of digitoxin by defining how it impinges on Orai1 to exert its suppressive effect on SOCE. Read More

View Article

Download full-text PDF

Source
http://www.eurekaselect.com/167294/article
Publisher Site
http://dx.doi.org/10.2174/1566524018666181113111316DOI Listing
January 2018
8 Reads
3.621 Impact Factor

Establishment of Retinal Degeneration Model in Rat and Monkey by Intravitreal Injection of Sodium Iodate.

Curr Mol Med 2018 ;18(6):352-364

Department of Ophthalmology of Shanghai Tenth People's Hospital, and Laboratory of Clinical Visual Science of Tongji Eye Institute, and Department of Pharmacology, and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China.

Background: Animal models play critical roles in studies of the etiology and therapy of retinal degeneration (RD).

Objective: To establish an RD model without severe systemic side effects in monkeys.

Methods: Cynomolgus monkeys and Sprague-Dawley rats were treated with intravenous and intravitreal sodium iodate (SI). Read More

View Article

Download full-text PDF

Source
https://benthamscience.com/journals/current-molecular-medici
Web Search
http://www.eurekaselect.com/167291/article
Publisher Site
http://dx.doi.org/10.2174/1566524018666181113104023DOI Listing
January 2018
11 Reads
3.621 Impact Factor

Enhanced Resistance of Triploid Crucian Carp to Cadmiuminduced Oxidative and Endoplasmic Reticulum Stresses.

Curr Mol Med 2018 ;18(6):400-408

State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, 410081, Hunan, China.

Background: Cadmium is a major heavy metal pollutant. Even at low concentrations in waste water Cadmium can accumulate in algae and sediments, and it is absorbed by both plants and aquatic animals, like shellfish and fish. Triploid crucian carp is an important economic fish, and have been farmed on a large scale in China. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524018666181113105018DOI Listing
January 2018
8 Reads

Role of HIF-1 in Cancer Progression: Novel Insights. A Review.

Curr Mol Med 2018 ;18(6):343-351

Department of Clinical and Molecular Medicine, S. Andrea Hospital -Sapienza University, Rome, Italy.

Hypoxia is a condition always present in tumor environment owing to the fast growth of tumor cells not supported by adequate blood supply. There is increasing evidence that hypoxia plays an important role in cancer dormancy and cancer metabolism, increasing stemness activity and bringing about cancer initiation and progression. This condition may influence the production of hypoxia inducible factor (HIF) a helix transcription factor which is involved in carcinogenesis and tumor growth through the regulation of genes involved in angiogenesis, glycolytic metabolism and other biological mechanisms. Read More

View Article

Download full-text PDF

Source
http://www.eurekaselect.com/167204/article
Publisher Site
http://dx.doi.org/10.2174/1566524018666181109121849DOI Listing
January 2018
11 Reads

Research of Gene Delivery Mediated by Ultrasound, Microbubble and Folate-modified Chitosan Nanoparticles.

Curr Mol Med 2018 ;18(6):383-391

Department of Ultrasound Medicine, Laboratory of Ultrasound Molecular Imaging, The Third Affiliated Hospital of Guangzhou Medical University, The Liwan Hospital of the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Objective: To study transfection efficiency of folate-modified chitosan (FACS) nanoparticles as a non-viral vector delivering pEGFP-C3plasmid (FA-CS/P) to 293T cells with or without the combination of ultrasound and microbubble.

Method: pEGFP-C3 was used as a reporter gene and FA-CS nanoparticles, which were prepared by complex coagulation method, were used as biological carriers. Transfection efficiency to 293T cells mediated by FA-CS/P nanoparticles, ultrasound (US) and microbubble (MB) was assessed by fluorescence microscopy and flow cytometry. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524018666181109121509DOI Listing
January 2018
11 Reads

Comparison of Small-incision Femtosecond Laser-assisted Intrastromal Keratoplasty and Lamellar Keratoplasty in Rhesus Monkeys Using Xenogenic Corneal Lamellae.

Curr Mol Med 2018 ;18(6):365-375

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University. Guangzhou 510060, China.

Background: In our previous study, both allogeneic and xenogeneic smallincision femtosecond laser-assisted intrastromal keratoplasty were demonstrated to be safe and effective surgeries. Glycerol-dehydrated corneal lamellae could serve as alternative xenogeneic inlay grafts. However, these xenogeneic lamellae have not been explored in lamellar keratoplasty (LK). Read More

View Article

Download full-text PDF

Source
http://www.eurekaselect.com/167201/article
Publisher Site
http://dx.doi.org/10.2174/1566524018666181109120023DOI Listing
January 2018
17 Reads
3.620 Impact Factor

Mutation Analysis of Pre-mRNA Splicing Genes PRPF31, PRPF8, and SNRNP200 in Chinese Families with Autosomal Dominant Retinitis Pigmentosa.

Curr Mol Med 2018 ;18(5):287-294

Department of Ophthalmology of Shanghai Tenth People's Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China.

Background: To screen variants in pre-mRNA Splicing genes in 95 Chinese autosomal dominant retinitis pigmentosa (adRP) families.

Methods: Clinical examination and pedigree analysis were performed. Targeted exome sequencing (TES) and / or Sanger sequencing were performed to detect the variants in genes of Splicing factors and conduct intra-familiar segregation analysis with DNA available. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524018666181024160452DOI Listing
January 2018
16 Reads

Excessive Oxidative Stress in the Synergistic Effects of Shikonin on the Hyperthermia-Induced Apoptosis.

Curr Mol Med 2018 ;18(5):322-334

Department of Public Health, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

Background: Hyperthermia (HT) has been used widely for cancer therapy, and the development of modern devices has made it more efficient. Shikonin (SHK) is a natural naphthoquinone derivative from a Chinese herb. Although the anticancer properties of SHK are evident, the underlying molecular mechanisms are not fully understood. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524018666181024161704DOI Listing
January 2018
1 Read

Utilization of Analytical Omics Tools in the Molecular Diagnostics of Multiple Myeloma.

Authors:
Z Kovacs A Guttman

Curr Mol Med 2018 ;18(4):260-272

Horvath Csaba Laboratory of Bioseparation Sciences, Research Centre for Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen 4032, Hungary.

Multiple myeloma (MM) is characterized by the clonal proliferation of malignant plasma B-lymphocytes and even as of today, it is an incurable disease. MM accounts for approximately 10% of all hematologic cancers. Its molecular pathogenesis is poorly understood, but the bone marrow microenvironment of tumor cells and genetic factors have apparent roles in the process. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524018666181008112738DOI Listing
January 2018
14 Reads

VEGFR2 and VEGF-C Suppresses the Epithelial-Mesenchymal Transition Via YAP in Retinal Pigment Epithelial Cells.

Curr Mol Med 2018 ;18(5):273-286

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.

Background: Whereas retinal pigment epithelial (RPE) cells are known to secrete VEGF-A and VEGFR2, the functions of the autocrine VEGF signaling remain unclear. Meanwhile, anti-VEGF therapies have been applied routinely to treat ocular vascular diseases.

Objective: The aim of this study was to determine the functions of the VEGF signaling in RPE cells and evaluate the consequences of its interruption. Read More

View Article

Download full-text PDF

Source
http://www.eurekaselect.com/165946/article
Publisher Site
http://dx.doi.org/10.2174/1566524018666181004115304DOI Listing
January 2018
15 Reads

Probable Mechanisms Involved in Immunotoxin Mediated Capillary Leak Syndrome (CLS) and Recently Developed Countering Strategies.

Curr Mol Med 2018 ;18(5):335-342

Recombinant Proteins Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran.

Antibody-toxin fused agents or immunotoxins, are a newly engineered class of cytotoxic agents consisting of a bacterial or plant toxin moiety hooked up either to a monoclonal antibody or a specific growth factor. Nevertheless, acquiring a full potency in clinic is mostly restricted due to the Capillary leak syndrome (CLS), a serious immune provoked, life-threatening side effect, subsequent to the endothelial damage, resulting in fluid escape from the bloodstream into tissues including lungs, muscle and brain, developing organ failure and eventually death. Proposed underlying mechanisms include direct damage to endothelial cells, acute inflammation, Lymphokine-activated killer (LAK) cells engagement, alteration in cell-cell/cell-matrix connectivities and cytoskeletal dysfunction. Read More

View Article

Download full-text PDF

Source
http://www.eurekaselect.com/165948/article
Publisher Site
http://dx.doi.org/10.2174/1566524018666181004120112DOI Listing
January 2018
14 Reads

MiR-19b Functions as a Potential Protector in Experimental Autoimmune Encephalomyelitis.

Curr Mol Med 2018 ;18(5):312-321

Department of Neurology, Xiangya Hospital of Central South University, Changsha, 410008, China.

Background: MicroRNA-19b (miR-19b) is essential in determining oligodendroglia proliferation. Phosphatase and tensin homologue on chromosome 10 (PTEN) is considered the target of miR-19b and participates in oligodendrocyte differentiation and proliferation.

Methods: Murine EAE was induced by myelin oligodendrocyte glycoprotein (MOG35- 55). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524018666181004123716DOI Listing
January 2018
1 Read

Genetic Modifiers of Fetal Haemoglobin (HbF) and Phenotypic Severity in β-Thalassemia Patients.

Curr Mol Med 2018 ;18(5):295-305

Department of Paediatrics, Faculty of Medicine, University Kebangsaan Malaysia (UKM), Jalan Yaacob Latif, 56000 Cheras, Kuala Lumpur, Malaysia.

Background: The phenotypic severity of β-thalassemia is highly modulated by three genetic modifiers: β-globin (HBB) mutations, co-inheritance of α-thalassemia and polymorphisms in the genes associated with fetal haemoglobin (HbF) production. This study was aimed to evaluate the effect of HbF related polymorphisms mainly in the HBB cluster, BCL11A (B-cell CLL/lymphoma 11A) and HBS1L-MYB (HBS1-like translational GTPase-MYB protooncogene, transcription factor) with regards to clinical severity.

Methods: A total of 149 patients were included in the study. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524018666181004121604DOI Listing
January 2018
11 Reads

Coding and Non-coding: Molecular Portrait of GIST and its Clinical Implication.

Curr Mol Med 2018 ;18(4):252-259

Medical Genetics Laboratory, Institute of Molecular Medicine, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation.

Gastrointestinal stromal tumours are the most common mesenchymal tumours of the gastrointestinal tract. Despite similar mutation pattern of activating mutations in KIT or PDGFRA receptors in 85% of cases, they demonstrate significantly heterogeneous clinical behaviour and pathological characteristics. This heterogeneity opens the question of the role of other factors and mechanisms of regulation in the development of GIST. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524018666181004113436DOI Listing
January 2018
1 Read

Co-Existence of Novel PDE6A Mutations and A Recurrent RPGR Mutation: A Potential Explanation for Phenotypic Diversity in Female RPGR Mutation Carriers.

Curr Mol Med 2018 ;18(5):306-311

Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing 210029, China.

Background: To report the co-existence of novel biallelic PDE6A mutations and heterozygous RPGR mutation in a Chinese female patient with retinitis pigmentosa (RP), and to analyze the intrafamilial phenotypic diversity.

Methods: Three patients with retinopathy and four healthy family members were included in genetic and clinical analyses. Personal medical records were obtained from another four unaffected female family members who refused blood donation. Read More

View Article

Download full-text PDF

Source
http://www.eurekaselect.com/165950/article
Publisher Site
http://dx.doi.org/10.2174/1566524018666181004122607DOI Listing
January 2018
5 Reads

Bone Minimodeling, a Special Modeling Pattern and Potential as Therapeutic Target for Osteoporosis.

Authors:
X Li J Zhang X Wang

Curr Mol Med 2018 ;18(4):233-240

Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, Shandong Province 250012, China.

The concept of minimodeling is defined as a kind of focal bone formation that features the absence of preceding bone absorption by osteoclasts. In the process of minimodeling, osteogenetic and osteoclastic activities are decoupled. Linear boundary between old bone and new bone can be discovered. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524018666181004113128DOI Listing
January 2018
1 Read

Role of Mammalian Target of Rapamycin in Atherosclerosis.

Authors:
Z Cai Y He Y Chen

Curr Mol Med 2018 ;18(4):216-232

Department of Neurology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

The mammalian target of rapamycin (mTOR) regulates multiple pathophysiological processes, such as cell development, angiogenesis, autophagy, as well as innate-adaptive immune responses. Numerous studies have demonstrated that mTOR signaling plays an important role in the process of atherosclerosis (AS) itself or AS-related diseases. The activation of mTOR signaling contributes to the endothelium dysfunction and the formation of foam cells via enhancing the process from monocyte to macrophage in the initial stage of atherosclerosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1566524018666180926163917DOI Listing
January 2018
1 Read