3,755 results match your criteria Current Genetics [Journal]


Tuning the sequence specificity of a transcription terminator.

Curr Genet 2019 Feb 9. Epub 2019 Feb 9.

Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.

The bacterial hexameric helicase known as Rho is an archetypal sequence-specific transcription terminator that typically halts the synthesis of a defined set of transcripts, particularly those bearing cytosine-rich 3'-untranslated regions. However, under conditions of translational stress, Rho can also terminate transcription at cytosine-poor sites when assisted by the transcription factor NusG. Recent structural, biochemical, and computational studies of the Rho·NusG interaction in Escherichia coli have helped establish how NusG reprograms Rho activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-019-00939-1DOI Listing
February 2019

An aggregation-prone mutant of eIF3a forms reversible assemblies escaping spatial control in exponentially growing yeast cells.

Curr Genet 2019 Feb 4. Epub 2019 Feb 4.

Laboratory of Cell Reproduction, Institute of Microbiology of the CAS, Videnska 1083, 14220, Prague 4, Czech Republic.

Cells have elaborated a complex strategy to maintain protein homeostasis under physiological as well as stress conditions with the aim to ensure the smooth functioning of vital processes and producing healthy offspring. Impairment of one of the most important processes in living cells, translation, might have serious consequences including various brain disorders in humans. Here, we describe a variant of the translation initiation factor eIF3a, Rpg1-3, mutated in its PCI domain that displays an attenuated translation efficiency and formation of reversible assemblies at physiological growth conditions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-019-00940-8DOI Listing
February 2019

Whi2: a new player in amino acid sensing.

Curr Genet 2019 Jan 30. Epub 2019 Jan 30.

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, 21205, USA.

A critical function of human, yeast, and bacterial cells is the ability to sense and respond to available nutrients such as glucose and amino acids. Cells must also detect declining nutrient levels to adequately prepare for starvation conditions by inhibiting cell growth and activating autophagy. The evolutionarily conserved protein complex TORC1 regulates these cellular responses to nutrients, and in particular to amino acid availability. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-00929-9
Publisher Site
http://dx.doi.org/10.1007/s00294-018-00929-9DOI Listing
January 2019
5 Reads

Synergy of Hir1, Ssn6, and Snf2 global regulators is the functional determinant of a Mac1 transcriptional switch in S. cerevisiae copper homeostasis.

Curr Genet 2019 Jan 28. Epub 2019 Jan 28.

Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, N. Plastira 100, 700 13, Heraklion, Crete, Greece.

To gain insights on the transcriptional switches that modulate proper copper homeostasis in yeast, we have examined in detail functional interactions of the relevant transcriptional activator Mac1. We identified Hir1 transcriptional repressor and histone chaperone as a Mac1-interacting protein. This association directly recruits Hir1 on a Mac1 target, CTR1 promoter, quantitatively under induction conditions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-019-00935-5DOI Listing
January 2019

Basal transcription profiles of the rhamnose-inducible promoter P and the development of efficient P-based systems for markerless gene deletion and a mutant library in Pichia pastoris.

Curr Genet 2019 Jan 24. Epub 2019 Jan 24.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing, 100081, China.

An ideal inducible promoter presents inducibility with an inducer and no basal transcription without inducer. Previous studies have shown that P in Pichia pastoris is a strong rhamnose-inducible promoter for driving the industrial production of recombinant proteins. However, another important profile of P, the basal transcription, was not investigated yet. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-019-00934-6DOI Listing
January 2019
2.682 Impact Factor

Down-regulation of Cdk1 activity in G1 coordinates the G1/S gene expression programme with genome replication.

Curr Genet 2019 Jan 24. Epub 2019 Jan 24.

Institute of Functional Biology and Genomics, CSIC, University of Salamanca, 37007, Salamanca, Spain.

Cell division is regulated by cyclin-dependent kinases (Cdks) and requires the periodic activation and inactivation of transcription factors that generate waves of gene expression in different cell-cycle phases. In fission yeast, the MCB-binding transcription factor (MBF) is activated at the end of G1 and regulates the expression of a set of genes that encode for proteins involved in the G1/S transition and DNA replication. Here, we review the importance of controlling MBF by Cdk activity at the onset of S phase. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-00926-yDOI Listing
January 2019

Is Gcn4-induced autophagy the ultimate downstream mechanism by which hormesis extends yeast replicative lifespan?

Curr Genet 2019 Jan 23. Epub 2019 Jan 23.

Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, 10065, USA.

The number of times a cell divides before irreversibly arresting is termed replicative lifespan. Despite discovery of many chemical, dietary and genetic interventions that extend replicative lifespan, usually first discovered in budding yeast and subsequently shown to apply to metazoans, there is still little understanding of the underlying molecular mechanisms involved. One unifying theme is that most, if not all, interventions that extend replicative lifespan induce "hormesis", where a little inflicted damage makes cells more able to resist similar challenges in the future. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-019-00936-4DOI Listing
January 2019

The meiotic-specific Mek1 kinase in budding yeast regulates interhomolog recombination and coordinates meiotic progression with double-strand break repair.

Curr Genet 2019 Jan 22. Epub 2019 Jan 22.

Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, 11794, USA.

Recombination, along with sister chromatid cohesion, is used during meiosis to physically connect homologous chromosomes so that they can be segregated properly at the first meiotic division. Recombination is initiated by the introduction of programmed double strand breaks (DSBs) into the genome, a subset of which is processed into crossovers. In budding yeast, the regulation of meiotic DSB repair is controlled by a meiosis-specific kinase called Mek1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-019-00937-3DOI Listing
January 2019

On the duration of the microbial lag phase.

Curr Genet 2019 Jan 21. Epub 2019 Jan 21.

VIB Laboratory for Systems Biology, VIB-KU Leuven Center for Microbiology, Gaston Geenslaan 1, 3001, Leuven, Belgium.

When faced with environmental changes, microbes enter a lag phase during which cell growth is arrested, allowing cells to adapt to the new situation. The discovery of the lag phase started the field of gene regulation and led to the unraveling of underlying mechanisms. However, the factors determining the exact duration and dynamics of the lag phase remain largely elusive. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-019-00938-2
Publisher Site
http://dx.doi.org/10.1007/s00294-019-00938-2DOI Listing
January 2019
4 Reads

Disturbance in biosynthesis of arachidonic acid impairs the sexual development of the onion blight pathogen Stemphylium eturmiunum.

Curr Genet 2019 Jan 16. Epub 2019 Jan 16.

Key Laboratory for Biology of Vegetable Diseases and Insect Pests of Shandong Province, Department of Plant Pathology, Shandong Agricultural University, Taian, 271018, Shandong, China.

The formation of sexual fruiting bodies for plant pathogenic fungi is a key strategy to propagate their progenies upon environmental stresses. Stemphylium eturmiunum is an opportunistic plant pathogen fungus causing blight in onion. This self-fertilizing filamentous ascomycete persists in the soil by forming pseudothecia, the sexual fruiting body which helps the fungus survive in harsh environments. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-019-00930-w
Publisher Site
http://dx.doi.org/10.1007/s00294-019-00930-wDOI Listing
January 2019
6 Reads

Quiescence, an individual journey.

Curr Genet 2019 Jan 16. Epub 2019 Jan 16.

Centre National de la Recherche Scientifique, Institut de Biochimie et Génétique Cellulaires, Unité Mixte de Recherche 5095, Université de Bordeaux, CS61390, Bordeaux Cedex, 33077, France.

Quiescence is operationally characterized as a temporary and reversible proliferation arrest. There are many preconceived ideas about quiescence, quiescent cells being generally viewed as insignificant sleeping G1 cells. In fact, quiescence is central for organism physiology and its dysregulation involved in many pathologies. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-00928-wDOI Listing
January 2019
1 Read

Role of Mediator in virulence and antifungal drug resistance in pathogenic fungi.

Curr Genet 2019 Jan 14. Epub 2019 Jan 14.

Division of Oral Biosciences, Dublin Dental University Hospital, Dublin, Ireland.

Mediator complex has recently emerged as an important regulator of gene expression in pathogenic fungi. Mediator is a multi-subunit complex of polypeptides involved in transcriptional activation in eukaryotes, with roles including preinitiation complex (PIC) assembly and chromatin remodeling. Within the last decade, Mediator has been shown to play an integral role in regulating virulence gene expression and drug resistance in human fungal pathogens. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-019-00932-8DOI Listing
January 2019
1 Read

Adaptation in replicative senescence: a risky business.

Curr Genet 2019 Jan 12. Epub 2019 Jan 12.

Sorbonne Université, PSL Research University, CNRS, UMR8226, Institut de Biologie Physico-Chimique, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, 75005, Paris, France.

Cell proliferation is tightly regulated to avoid propagating DNA damage and mutations, which can lead to pathologies such as cancer. To ensure genome integrity, cells activate the DNA damage checkpoint in response to genotoxic lesions to block cell cycle progression. This surveillance mechanism provides time to repair the damage before resuming cell cycle with an intact genome. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-019-00933-7DOI Listing
January 2019
1 Read

Fusarium oxysporum f. sp. lycopersici CH transcription factor FolCzf1 is required for conidiation, fusaric acid production, and early host infection.

Curr Genet 2019 Jan 10. Epub 2019 Jan 10.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.

The soil-borne, asexual fungus Fusarium oxysporum f.sp. lycopersici (Fol) is a causal agent of tomato wilt disease. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-019-00931-9DOI Listing
January 2019
1 Read

Versatility of the Mec1 signaling network in mediating resistance to replication, genotoxic, and proteotoxic stresses.

Curr Genet 2019 Jan 5. Epub 2019 Jan 5.

School of Medical Sciences and North West Cancer Research Institute, Bangor University, Bangor, LL57 2UW, UK.

The ataxia-telangiectasia mutated/ATM and Rad3-related (ATM/ATR) family proteins are evolutionarily conserved serine/threonine kinases best known for their roles in mediating the DNA damage response. Upon activation, ATM/ATR phosphorylate numerous targets to stabilize stalled replication forks, repair damaged DNA, and inhibit cell cycle progression to ensure survival of the cell and safeguard integrity of the genome. Intriguingly, separation of function alleles of the human ATM and MEC1, the budding yeast ATM/ATR, were shown to confer widespread protein aggregation and acute sensitivity to different types of proteotoxic agents including heavy metal, amino acid analogue, and an aggregation-prone peptide derived from the Huntington's disease protein. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0920-y
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0920-yDOI Listing
January 2019
4 Reads

More than just a phase: the search for membraneless organelles in the bacterial cytoplasm.

Curr Genet 2019 Jan 2. Epub 2019 Jan 2.

Department of Biology, University of Rochester, Rochester, NY, USA.

The bacterial cytoplasm, once thought to be a relatively undifferentiated reaction medium, has now been recognized to have extensive microstructure. This microstructure includes bacterial microcompartments, inclusion bodies, granules, and even some membrane-bound vesicles. Several recent papers suggest that bacteria may also organize their cytoplasm using an additional mechanism: phase-separated membraneless organelles, a strategy commonly used by eukaryotes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-00927-xDOI Listing
January 2019
1 Read

FgPEX4 is involved in development, pathogenicity, and cell wall integrity in Fusarium graminearum.

Curr Genet 2019 Jan 2. Epub 2019 Jan 2.

Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai'an, 271018, China.

Peroxisomes are indispensable organelles that play critical roles in various biological processes in eukaryotic cells. PEX4, one of the peroxins, is the ubiquitin-conjugating enzyme. To functionally characterize roles of FgPEX4 in the phytopathogenic fungus, Fusarium graminearum, we constructed a deletion mutant of FgPEX4 (ΔPEX4) through homologous recombination. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0925-6DOI Listing
January 2019
1 Read

Transcription factor CCG-8 plays a pivotal role in azole adaptive responses of Neurospora crassa by regulating intracellular azole accumulation.

Curr Genet 2019 Jan 2. Epub 2019 Jan 2.

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing, 100101, People's Republic of China.

Azoles are the most widely used antifungals for controlling fungal infections in clinic and agriculture. Fungi can adapt to azole stress by rapidly activating the transcription of a number of genes, and some of these genes can elevate resistance to azoles. We had reported the transcription factor CCG-8 as a new regulator in the adaptation to antifungal azole stress in Neurospora crassa and Fusarium verticillioides. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0924-7
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0924-7DOI Listing
January 2019
3 Reads

Linking the organization of DNA replication with genome maintenance.

Curr Genet 2019 Jan 2. Epub 2019 Jan 2.

CNRS, University of Rennes, Institute of Genetics and Development of Rennes, 35043, Rennes, France.

The spatial and temporal organization of genome duplication, also referred to as the replication program, is defined by the distribution and the activities of the sites of replication initiation across the genome. Alterations to the replication profile are associated with cell fate changes during development and in pathologies, but the importance of undergoing S phase with distinct and specific programs remains largely unexplored. We have recently addressed this question, focusing on the interplay between the replication program and genome maintenance. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0923-8DOI Listing
January 2019
1 Read

Recruitment, loading, and activation of the Smc5-Smc6 SUMO ligase.

Curr Genet 2019 Jan 2. Epub 2019 Jan 2.

Department of Molecular Medicine, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA, 92037, USA.

Duplication of the genome poses one of the most significant threats to genetic integrity, cellular fitness, and organismal health. Therefore, numerous mechanisms have evolved that maintain replication fork stability in the face of DNA damage and allow faithful genome duplication. The fission yeast BRCT-domain-containing protein Brc1, and its budding yeast orthologue Rtt107, has emerged as a "hub" factor that integrates multiple replication fork protection mechanisms. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0922-9DOI Listing
January 2019
1 Read

Spatiotemporal regulation of the Dma1-mediated mitotic checkpoint coordinates mitosis with cytokinesis.

Curr Genet 2019 Jan 2. Epub 2019 Jan 2.

Vanderbilt University School of Medicine, Nashville, USA.

During cell division, the timing of mitosis and cytokinesis must be ordered to ensure that each daughter cell receives a complete, undamaged copy of the genome. In fission yeast, the septation initiation network (SIN) is responsible for this coordination, and a mitotic checkpoint dependent on the E3 ubiquitin ligase Dma1 and the protein kinase CK1 controls SIN signaling to delay cytokinesis when there are errors in mitosis. The participation of kinases and ubiquitin ligases in cell cycle checkpoints that maintain genome integrity is conserved from yeast to human, making fission yeast an excellent model system in which to study checkpoint mechanisms. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0921-xDOI Listing
January 2019
3 Reads

Correction to: Novel genetic tools for probing individual H3 molecules in each nucleosome.

Curr Genet 2018 Dec 19. Epub 2018 Dec 19.

Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA, 01605, USA.

In the original publication, Fig. 1 was incorrectly published. The amino acid sequence was shifted to the left relative to the rest of the diagram in the published version and the corrected figure is given here. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0919-4
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0919-4DOI Listing
December 2018
5 Reads

SET domains and stress: uncovering new functions for yeast Set4.

Curr Genet 2018 Dec 6. Epub 2018 Dec 6.

Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD, 21250, USA.

Chromatin dynamics are central to the regulation of gene expression and genome stability, particularly in the presence of environmental signals or stresses that prompt rapid reprogramming of the genome to promote survival or differentiation. While numerous chromatin regulators have been implicated in modulating cellular responses to stress, gaps in our mechanistic understanding of chromatin-based changes during stress suggest that additional proteins are likely critical to these responses and the molecular details underlying their activities are unclear in many cases. We recently identified a role for the relatively uncharacterized SET domain protein Set4 in promoting cell survival during oxidative stress in Saccharomyces cerevisiae. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0917-6DOI Listing
December 2018
2 Reads

Multifaceted activities of DNA polymerase η: beyond translesion DNA synthesis.

Curr Genet 2018 Dec 8. Epub 2018 Dec 8.

Laboratory of Genomic Instability and Diseases, Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, 751023, India.

DNA polymerases are evolved to extend the 3'-OH of a growing primer annealed to a template DNA substrate. Since replicative DNA polymerases have a limited role while replicating structurally distorted template, translesion DNA polymerases mostly from Y-family come to the rescue of stalled replication fork and maintain genome stability. DNA polymerase eta is one such specialized enzyme whose function is directly associated with casual development of certain skin cancers and chemo-resistance. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0918-5DOI Listing
December 2018
3 Reads

Novel insights into molecular chaperone regulation of ribonucleotide reductase.

Curr Genet 2018 Dec 5. Epub 2018 Dec 5.

Department of Biological Sciences, The University of North Carolina at Charlotte, Charlotte, NC, 28223, USA.

The molecular chaperones Hsp70 and Hsp90 bind and fold a significant proportion of the proteome. They are responsible for the activity and stability of many disease-related proteins including those in cancer. Substantial effort has been devoted to developing a range of chaperone inhibitors for clinical use. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0916-7DOI Listing
December 2018
9 Reads

Interactions in the ESCRT-III network of the yeast Saccharomyces cerevisiae.

Curr Genet 2018 Dec 1. Epub 2018 Dec 1.

Institut für Lebensmittelwissenschaft und Biotechnologie, Fg. Hefegenetik und Gärungstechnologie (150f), Universität Hohenheim, Garbenstr. 23, 70599, Stuttgart, Germany.

Here, we examine the genetic interactions between ESCRT-III mutations in the yeast Saccharomyces cerevisiae. From the obtained interaction network, we make predictions about alternative ESCRT-III complexes. By the successful generation of an octuple deletion strain using the CRISPR/Cas9 technique, we demonstrate for the first time that ESCRT-III activity as a whole is not essential for the life of a yeast cell. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0915-8
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0915-8DOI Listing
December 2018
9 Reads

Escaping nuclear decay: the significance of mRNA export for gene expression.

Curr Genet 2018 Dec 4. Epub 2018 Dec 4.

Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, Building, 1130, 8000, Aarhus C, Denmark.

In this perspective, we discuss the regulatory impact of nuclear RNA export and decay on messenger RNA (mRNA) functionality. It is well established that control of protein-coding gene expression in eukaryotes employs the regulated production of mRNA, its intra-cellular transfer to cytoplasmic ribosomes and final transcript degradation. Despite a rich body of literature on these events, an involvement of nuclear RNA decay systems remains largely unexplored. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0913-x
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0913-xDOI Listing
December 2018
8 Reads

The Rio1 protein kinases/ATPases: conserved regulators of growth, division, and genomic stability.

Curr Genet 2018 Dec 4. Epub 2018 Dec 4.

Centre for Integrative Biology, University of Trento, Via Sommarive 9, 38123, Povo, TN, Italy.

The atypical Rio1 protein kinases/ATPases, which exist in most archaea and eukaryotes, have been studied intensely to understand how they promote small ribosomal subunit (SSU) maturation. However, mutant and knockdown phenotypes in various organisms suggested roles in activities beyond SSU biogenesis, including the regulation of cell cycle progression (DNA transcription, replication, condensation, and segregation), cell division, metabolism, physiology, and development. Recent work with budding yeast, indeed, revealed that Rio1 (RIOK1 in metazoans) manages a large signaling network at the protein and gene levels via which it stimulates or restricts growth and division in response to nutrient availability. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0912-y
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0912-yDOI Listing
December 2018
5 Reads

(Un)folding mechanisms of adaptation to ER stress: lessons from aneuploidy.

Curr Genet 2018 Dec 3. Epub 2018 Dec 3.

Department of Dermatology, Boston University School of Medicine, 609 Albany St, Rm J-501, Boston, MA, 02118, USA.

During stress, accumulation of misfolded proteins in the endoplasmic reticulum (ER) triggers activation of the adaptive mechanisms that restore protein homeostasis. One mechanism that eukaryotic cells use to respond to ER stress is through activation of the unfolded protein response (UPR) signaling pathway, which initiates degradation of misfolded proteins and leads to inhibition of translation and increased expression of chaperones and oxidative folding components that enhance ER protein folding capacity. However, the mechanisms of adaptation to ER stress are not limited to the UPR. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0914-9
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0914-9DOI Listing
December 2018
8 Reads

A strong structural correlation between short inverted repeat sequences and the polyadenylation signal in yeast and nucleosome exclusion by these inverted repeats.

Curr Genet 2018 Nov 29. Epub 2018 Nov 29.

Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo, 162-8480, Japan.

DNA sequences that read the same from 5' to 3' in either strand are called inverted repeat sequences or simply IRs. They are found throughout a wide variety of genomes, from prokaryotes to eukaryotes. Despite extensive research, their in vivo functions, if any, remain unclear. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0907-8
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0907-8DOI Listing
November 2018
11 Reads

A helicase links upstream ORFs and RNA structure.

Curr Genet 2018 Nov 27. Epub 2018 Nov 27.

Center for RNA Science and Therapeutics, School of Medicine, Case Western Reserve University, Cleveland, OH, 44106, USA.

Upstream open reading frames (uORFs) in 5' UTRs of eukaryotic mRNAs are increasingly recognized as important elements that regulate cellular protein synthesis. Since uORFs can start from non-AUG codons, an enormous number of potential uORF initiation sites exists in 5'UTRs. However, only a subset of these sites is used and it has been unclear how actual start sites are selected. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0911-zDOI Listing
November 2018
1 Read

Novel genetic tools for probing individual H3 molecules in each nucleosome.

Curr Genet 2018 Nov 26. Epub 2018 Nov 26.

Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA, 01605, USA.

In eukaryotes, genomic DNA is packaged into the nucleus together with histone proteins, forming chromatin. The fundamental repeating unit of chromatin is the nucleosome, a naturally symmetric structure that wraps DNA and is the substrate for numerous regulatory post-translational modifications. However, the biological significance of nucleosomal symmetry until recently had been unexplored. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0910-0
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0910-0DOI Listing
November 2018
10 Reads

Involvement of the two L-lactate dehydrogenase in development and pathogenicity in Fusarium graminearum.

Curr Genet 2018 Nov 24. Epub 2018 Nov 24.

Plant Protection Station of Pizhou City, Pizhou, 221300, Jiangsu, China.

Lactate dehydrogenase (LDH) widely exists in organisms, which catalyzes the interconversion of pyruvate into lactate with concomitant interconversion of NADH and NAD. In this study, two L-type lactate dehydrogenase genes FgLDHL1 and FgLDHL2 were characterized in an ascomycete fungus Fusarium graminearum, a causal agent of wheat head blight. Both the single-gene deletion mutants of FgLDHL1 or FgLDHL2 exhibited phenotypic defects in vegetative growth, sporulation, spore germination, L-lactate biosynthesis and activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0909-6DOI Listing
November 2018
4 Reads

DDR Inc., one business, two associates.

Curr Genet 2018 Nov 22. Epub 2018 Nov 22.

Equipe Labellisée Ligue contre le Cancer, Institut de Génétique Humaine, CNRS and Université de Montpellier, Montpellier, France.

Eukaryotic cells activate cell cycle checkpoints in response to DNA damage. In Saccharomyces cerevisiae, the DNA damage response is achieved by the activation of the sensor kinases Mec1 and Tel1 and transmitted to the effector kinase Rad53. Rad9 and Mrc1 are thought to differentially mediate the activation of Rad53 depending on the cell cycle phase. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0908-7DOI Listing
November 2018
2 Reads

Co-evolution of spliceosomal disassembly interologs: crowning J-protein component with moonlighting RNA-binding activity.

Curr Genet 2018 Nov 22. Epub 2018 Nov 22.

Chaperone and Stress Biology Lab, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Bhopal, Room Number 117, AB3, Bhopal Bypass Road, Bhauri, Bhopal, Madhya Pradesh, 462066, India.

Spliceosome disassembly is catalyzed by the NineTeen-related (NTR) complex, which is constituted by several proteins, including Cwc23, Ntr1, and Ppr43. Cwc23 is an essential J-protein in Saccharomyces cerevisiae that recruits Ntr1, an NTC-related G-patch protein, to the spliceosome. Ntr1 interacts with Prp43, a DExD/H box RNA helicase protein, which facilitates the disassembly of spliceosomal intermediates. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0906-9
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0906-9DOI Listing
November 2018
1 Read

Genome sequence of the opportunistic human pathogen Magnusiomyces capitatus.

Curr Genet 2018 Nov 19. Epub 2018 Nov 19.

Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovakia.

The yeast Magnusiomyces capitatus is an opportunistic human pathogen causing rare yet severe infections, especially in patients with hematological malignancies. Here, we report the 20.2 megabase genome sequence of an environmental strain of this species as well as the genome sequences of eight additional isolates from human and animal sources providing an insight into intraspecies variation. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0904-y
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0904-yDOI Listing
November 2018
7 Reads

ChECing out Rif1 action in freely cycling cells.

Curr Genet 2018 Nov 19. Epub 2018 Nov 19.

Department of Molecular Biology, University of Geneva, Geneva, Switzerland.

In buddying yeast, like all eukaryotes examined so far, DNA replication is under temporal control, such that some origins fire early and some late during S phase. This replication timing program is established in G1 phase, where chromatin states are thought to prevent binding of key-limiting initiation factors at late-firing origins. Although many factors are involved in replication initiation, a new player, Rif1, has recently entered the scene, with a spate of papers revealing a global role for the protein in the control of replication initiation timing from yeasts to humans. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0902-0
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0902-0DOI Listing
November 2018
13 Reads
2.682 Impact Factor

Structural modules of the stress-induced protein HflX: an outlook on its evolution and biological role.

Curr Genet 2018 Nov 17. Epub 2018 Nov 17.

Structural Biology and Bio-Informatics Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata, 700 032, India.

Multifunctional proteins often show modular structures. A functional domain and the structural modules within the domain show evolutionary conservation of their spatial arrangement since that gives the protein its functionality. However, the question remains as to how members of different domains of life (Archaea, Bacteria, Eukarya), polish and perfect these modules within conserved multidomain proteins, to tailor functional proteins according to their specific requirements. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0905-x
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0905-xDOI Listing
November 2018
7 Reads

Chromatin architecture and virulence-related gene expression in eukaryotic microbial pathogens.

Curr Genet 2018 Nov 15. Epub 2018 Nov 15.

División de Biología Molecular, IPICYT, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, 78216, San Luis Potosí, SLP, Mexico.

A fundamental question in biology is to understand how appropriate transcriptional regulation and dense packaging of the genetic material within the eukaryotic nucleus are achieved. The exquisite gene expression control and other metabolic processes of DNA require a highly complex, multilayered, three-dimensional architecture of the chromatin and its specific compartmentalization within the nucleus. Some of these architectural and sub-nuclear positioning mechanisms have been extensively co-opted by eukaryotic pathogens to keep fine expression control and expansion of virulence-related gene families in Plasmodium falciparum, Trypanosoma brucei and Candida glabrata. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0903-z
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0903-zDOI Listing
November 2018
11 Reads

Epigenetic fates of gene silencing established by heterochromatin spreading in cell identity and genome stability.

Curr Genet 2018 Nov 2. Epub 2018 Nov 2.

Department of Microbiology and Immunology, George Williams Hooper Foundation, University of California San Francisco, San Francisco, CA, 94143, USA.

Heterochromatin spreading, the propagation of repressive chromatin along the chromosome, is a reaction critical to genome stability and defense, as well as maintenance of unique cell fates. Here, we discuss the intrinsic properties of the spreading reaction and circumstances under which its products, formed distal to DNA-encoded nucleation sites, can be epigenetically maintained. Finally, we speculate that the epigenetic properties of heterochromatin evolved together with the need to stabilize cellular identity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0901-1DOI Listing
November 2018
1 Read

Mating-type switching by homology-directed recombinational repair: a matter of choice.

Curr Genet 2018 Oct 31. Epub 2018 Oct 31.

Institute of Innovative Research, Tokyo Institute of Technology, Tokyo, Japan.

In eukaryotes, all DNA transactions happen in the context of chromatin that often takes part in regulatory mechanisms. In particular, chromatin structure can regulate exchanges of DNA occurring through homologous recombination. Few systems have provided as detailed a view on this phenomenon as mating-type switching in yeast. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0900-2
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0900-2DOI Listing
October 2018
2 Reads

Stressing out or stressing in: intracellular pathways for SAPK activation.

Curr Genet 2018 Oct 30. Epub 2018 Oct 30.

Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, MA, 02118, USA.

Stress-activated MAP kinases (SAPKs) respond to a wide variety of stressors. In most cases, the pathways through which specific stress signals are transmitted to the SAPKs are not known. Our recent findings have begun to address two important and related questions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0898-5DOI Listing
October 2018
1 Read

Circadian rhythms, metabolic oscillators, and the target of rapamycin (TOR) pathway: the Neurospora connection.

Curr Genet 2018 Oct 26. Epub 2018 Oct 26.

Department of Biology, York University, Toronto, ON, M3J 1P3, Canada.

Circadian (24-h) rhythmicity is a fundamental property of eukaryotic cells, and it is not surprising that it intersects with fundamental metabolic processes. Many links between these two processes have been documented, and speculation has been growing that there may be circadian "metabolic oscillators" that interact with and exist independently of the well-known circadian transcription/translation feedback loops (TTFLs) that have been extensively studied. This review takes a critical look at the evidence for the existence of metabolic oscillators at the cellular level, attempting to answer these questions: does metabolism affect circadian rhythmicity, and vice versa? Is metabolism rhythmic, and if so, is that rhythmicity cell autonomous? Systems displaying "non-canonical rhythmicity" in the absence of functional TTFLs provide opportunities for identifying metabolic oscillators, and this review emphasizes the fungus Neurospora crassa as a model system. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0897-6
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0897-6DOI Listing
October 2018
6 Reads

Condensin action and compaction.

Curr Genet 2018 Oct 25. Epub 2018 Oct 25.

Department of Biology, New York University, New York, NY, 10003, USA.

Condensin is a multi-subunit protein complex that belongs to the family of structural maintenance of chromosomes (SMC) complexes. Condensins regulate chromosome structure in a wide range of processes including chromosome segregation, gene regulation, DNA repair and recombination. Recent research defined the structural features and molecular activities of condensins, but it is unclear how these activities are connected to the multitude of phenotypes and functions attributed to condensins. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0899-4
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0899-4DOI Listing
October 2018
11 Reads

Ordering up gene expression by slowing down transcription factor binding kinetics.

Authors:
Matthew J Culyba

Curr Genet 2018 Oct 23. Epub 2018 Oct 23.

Division of Infectious Diseases, Department of Medicine, University of Pittsburgh, 857 Scaife Hall, 3550 Terrace Street, Pittsburgh, PA, 15261, USA.

Efficient regulation of a complex genetic response requires that the gene products, which catalyze the response, be synthesized in a temporally ordered manner to match the sequential nature of the reaction pathway they act upon. Transcription regulation networks coordinate this aspect of cellular control by modulating transcription factor (TF) concentrations through time. The effect a TF has on the timing of gene expression is often modeled assuming that the TF-promoter binding reaction is in thermodynamic equilibrium with changes in TF concentration over time; however, non-equilibrium dynamics resulting from relatively slow TF-binding kinetics can result in different network behavior. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0896-7DOI Listing
October 2018
1 Read

Genetic mechanisms of arsenic detoxification and metabolism in bacteria.

Curr Genet 2018 Oct 22. Epub 2018 Oct 22.

Ministry of Education Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, School of Pharmaceutical Sciences, Zhongnan Hospital, Wuhan University, Wuhan, 430071, Hubei, China.

Arsenic, distributed pervasively in the natural environment, is an extremely toxic substance which can severely impair the normal functions of living cells. Research on the genetic mechanisms of arsenic metabolism is of great importance for remediating arsenic-contaminated environments. Many organisms, including bacteria, have developed various strategies to tolerate arsenic, by either detoxifying this harmful element or utilizing it for energy generation. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0894-9
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0894-9DOI Listing
October 2018
20 Reads

The etiology of uracil residues in the Saccharomyces cerevisiae genomic DNA.

Curr Genet 2018 Oct 17. Epub 2018 Oct 17.

Department of Microbiology and Molecular Genetics, University of Texas Health Science Center at Houston, Houston, TX, 77030, USA.

Non-canonical residue in DNA is a major and conserved source of genome instability. The appearance of uracil residues in DNA accompanies a significant mutagenic consequence and is regulated at multiple levels, from the concentration of available dUTP in the nucleotide pool to the excision repair for removal from DNA. Recently, an interesting phenomenon of transcription-associated elevation in uracil-derived mutations was described in Saccharomyces cerevisiae genome. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0895-8
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0895-8DOI Listing
October 2018
12 Reads

Correction to: Gene overexpression screen for chromosome instability in yeast primarily identifies cell cycle progression genes.

Curr Genet 2018 Oct 16. Epub 2018 Oct 16.

Institute of Environmental Sciences, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland.

In the original publication, 'Frumkin JP et al.' reference was missed to include in the reference list. The complete reference should read as below. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00294-018-0893-xDOI Listing
October 2018
2 Reads

Yeast two-hybrid screening reveals a dual function for the histone acetyltransferase GcnE by controlling glutamine synthesis and development in Aspergillus fumigatus.

Curr Genet 2018 Oct 15. Epub 2018 Oct 15.

Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute (HKI), Beutenbergstr. 11a, 07745, Jena, Germany.

The acetyltransferase GcnE is part of the SAGA complex which regulates fungal gene expression through acetylation of chromatin. Target genes of the histone acetyltransferase GcnE include those involved in secondary metabolism and asexual development. Here, we show that the absence of GcnE not only abrogated conidiation, but also strongly impeded vegetative growth of hyphae in the human pathogenic fungus Aspergillus fumigatus. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0891-z
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0891-zDOI Listing
October 2018
3 Reads

The nonsense-mediated mRNA decay (NMD) pathway differentially regulates COX17, COX19 and COX23 mRNAs.

Curr Genet 2018 Oct 13. Epub 2018 Oct 13.

Department of Biology, Baylor University, One Bear Place #97388, Waco, TX, 76798, USA.

The differential regulation of COX17, COX19 and COX23 mRNAs by the nonsense-mediated mRNA decay (NMD) pathway was investigated. The NMD pathway regulates mRNAs that aberrantly terminate translation. This includes mRNAs harboring premature translation termination codons and natural mRNAs. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00294-018-0892-y
Publisher Site
http://dx.doi.org/10.1007/s00294-018-0892-yDOI Listing
October 2018
11 Reads