12 results match your criteria Current Analytical Chemistry[Journal]

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Simultaneous Quantification of Citalopram and its Main Metabolite, 
Desmethylcitalopram, in Human Saliva by UHPLC.

Curr Anal Chem 2018 Dec;14(6):554-561

Department of Analytical Chemistry, Medical University of Gdansk, Gen. J. Hallera 107, 80-416Gdansk, Poland.

Background: This study was designed to develop a reliable method for simultaneous quantita-tion of Citalopram (CIT) and its main active metabolite, Desmethylcitalopram (DCIT), in saliva of patients undergoing treatment with CIT.

Methods: To compare two procedures of saliva purification, Solid-Phase (SPE) and Liquid-Liquid (LLE) extractions, saliva samples obtained from healthy volunteers were spiked with adequate quantities of CIT and DCIT. Different cartridges were used for SPE, while dichloromethane for LLE. Read More

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http://dx.doi.org/10.2174/1573411013666171113144352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249691PMC
December 2018
12 Reads

A Multivariate Control Chart Approach for Calibration Transfer between NIR Spectrometers for Simultaneous Determination of Rifampicin and Isoniazid in Pharmaceutical Formulation.

Curr Anal Chem 2018 Oct;14(5):488-494

Biological Chemistry and Chemometrics, Institute of Chemistry, Federal University of Rio Grande do Norte, Natal, Brazil.

Background: Multivariate transfer techniques have become a widely accepted concept over the past few years, since they avoid full recalibration procedures when instruments are changed to analyze a specific sample.

Objective: This paper reports a multivariate control chart transfer approach between two near infrared (NIR) spectrometers for simultaneous determination of rifampicin and isoniazid in pharmaceutical formu-lation using Direct Standardization (DS).

Method: The control charts are based on the calculation of Net Analyte Signal (NAS) models and the transfer samples are selected by the Kennard-Stone (KS) algorithm. Read More

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http://dx.doi.org/10.2174/1573411014666171212141909DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182936PMC
October 2018
2 Reads

Optimization and Validation of a Microscale Method to Assess 
α-Glucosidase Inhibition Activity.

Curr Anal Chem 2018 Oct;14(5):458-464

Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química Analítica, Monterrey, N. L., México.

Background: Microscale in vitro assays are fast, simple, and inexpensive, with reduced reagent quantities, waste, and experimental animal use. However, they have low reproducibility and low correlation with the results of in vivo models, possibly due to differences in precision and accuracy in methodologies between laboratories.

Objective: The objective was the optimization and validation of an in vitro assay, carried out on microscale, to assess the inhibition of α-glucosidase activity, which is indicative of antihyperglycemic activity. Read More

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http://dx.doi.org/10.2174/1573411013666170911154755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6142409PMC
October 2018
6 Reads
1.132 Impact Factor

Optimization and Validation of Two High-Throughput Methods Indicating Antiradical Activity.

Curr Anal Chem 2017 Dec;13(6):499-507

Universidad Autónoma de Nuevo León, Facultad de Medicina, Departamento de Química Analítica. Monterrey, N. L. México.

Background: The search for new natural or synthetic products with antioxidant activity is commonly based on methods that involve reduction of either 2,2-diphenyl-1-picrylhydrazyl (DPPH) or 2-2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). However, the reported values of the effective concentrations are highly variable, even in controls. Herein, we optimize and validate both meth-ods of determining antiradical activity. Read More

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http://dx.doi.org/10.2174/1573411013666170118111516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737003PMC
December 2017
12 Reads

The Occurrence of Veterinary Pharmaceuticals in the Environment: A Review.

Curr Anal Chem 2016 Jun;12(3):169-182

Department of Bacteriology, Kimron Veterinary Institute, Ministry of Agriculture, Bet Degan, Israel.

It is well known that there is a widespread use of veterinary pharmaceuticals and consequent release into different ecosystems such as freshwater bodies and groundwater systems. Furthermore, the use of organic fertilizers produced from animal waste manure has been also responsible for the occurrence of veterinary pharmaceuticals in agricultural soils. This article is a review of different studies focused on the detection and quantification of such compounds in environmental compartments using different analytical techniques. Read More

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http://dx.doi.org/10.2174/1573411012666151009193108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425647PMC
June 2016
17 Reads

Enantiomeric Profiling of Chiral Pharmacologically Active Compounds in the Environment with the Usage of Chiral Liquid Chromatography 
Coupled with Tandem Mass Spectrometry.

Curr Anal Chem 2016 Aug;12(4):303-314

Department of Chemistry, University of Bath, Bath, United Kingdom.

The issue of drug chirality is attracting increasing attention among the scientific community. The phenomenon of chirality has been overlooked in environmental research (environmental occurrence, fate and toxicity) despite the great impact that chiral pharmacologically active compounds (cPACs) can provoke on ecosystems. The aim of this paper is to introduce the topic of chirality and its implications in environmental contamination. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5024650PMC
http://dx.doi.org/10.2174/1573411012666151009195039DOI Listing
August 2016
34 Reads

A Derivative Method with Free Radical Oxidation to Predict Resveratrol Metabolites by Tandem Mass Spectrometry.

Curr Anal Chem 2015 Oct;11(4):300-306

Department of Biotechnology, Kaohsiung Medical University, Kaohsiung,Taiwan;; Institute of 
Biomedical Science, National Sun Yat-Sen University, Kaohsiung,Taiwan;;

In this study, we demonstrated an oxidative method with free radical to generate 3,5,4'-trihydroxy--stilbene (-resveratrol) metabolites and detect sequentially by an autosampler coupling with liquid chromatography electrospray ionization tandem mass spectrometer (LC-ESI-MS/MS). In this oxidative method, the free radical initiator, ammonium persulfate (APS), was placed in a sample bottle containing resveratrol to produce oxidative derivatives, and the reaction progress was tracked by autosampler sequencing. Resveratrol, a natural product with purported cancer preventative qualities, produces metabolites including dihydroresveratrol, 3,4'-dihydroxy--stilbene, lunularin, resveratrol monosulfate, and dihydroresveratrol monosulfate by free radical oxidation. Read More

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http://dx.doi.org/10.2174/1573411011666150515233817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003074PMC
October 2015
15 Reads

Analytical methods for quantitation of prenylated flavonoids from hops.

Curr Anal Chem 2013 Jan;9(1):71-85

Department of Medicinal Chemistry and Pharmacognosy, UIC/NIH Center for Botanical Dietary Supplements Research, College of Pharmacy, University of Illinois at Chicago, 833 S. Wood Street, Chicago, IL 60612-7231, USA.

The female flowers of hops ( L.) are used as a flavoring agent in the brewing industry. There is growing interest in possible health benefits of hops, particularly as estrogenic and chemopreventive agents. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3783999PMC
January 2013
17 Reads

Ion Mobility Spectrometry-Mass Spectrometry of Intrinsically Unfolded Proteins: Trying to Put Order into Disorder.

Curr Anal Chem 2013 Apr;9(2):181-191

Astbury Centre for Structural Molecular Biology, Faculty of Mathematics and Physical Sciences, University of Leeds, Leeds, LS2 9JT, UK.

Intrinsically disordered proteins do not adopt well-defined native structures and therefore present an intriguing challenge in terms of structural elucidation as they are relatively inaccessible to traditional approaches such as NMR and X-ray crystallography. Many members of this important group of proteins have a distinct biological function and frequently undergo a conformational change on binding to their physiological targets which can in turn modulate their function. Furthermore, many intrinsically unstructured proteins are associated with a wide range of major diseases including cancer and amyloid-related disorders. Read More

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http://dx.doi.org/10.2174/1573411011309020004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706957PMC
April 2013
15 Reads

Molecular Mass Characterization of Glycosaminoglycans with Different Degrees of Sulfation in Bioengineered Heparin Process by Size Exclusion Chromatography.

Curr Anal Chem 2012 Oct;8(4):506-511

Department of Biology, Rensselaer Polytechnic Institute, Troy, New York 12180, USA ; Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.

Different degrees of glycosaminoglycan sulfation result in their different charge densities. The charge density differences impact their migration behavior in polyacrylamide gel electrophoresis and size exclusion chromatography, two of the most common methods for determining relative molecular masses of polysaccharides. In this study, we investigated the feasibility of using commercially available heparin oligosaccharides as calibrants for measuring the relative molecular masses of intermediates in a bioengineered heparin process that have different levels of sulfation. Read More

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http://dx.doi.org/10.2174/157341112803216753DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3525092PMC
October 2012
13 Reads

Sequence Analysis of Native Oligosaccharides Using Negative ESI Tandem MS.

Curr Anal Chem 2009 Jul;5(3):225-237

Departments of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.

Carbohydrates exhibit many physiologically and pharmacologically important activities, yet their complicated structure and sequence pose major analytical challenges. Although their structural complexity makes analysis of carbohydrate difficult, mass spectrometry (MS) with high sensitivity, resolution and accuracy has become a vital tool in many applications related to analysis of carbohydrates or oligosaccharides. This application is essentially based on soft ionization technique which facilitates the ionization and vaporization of large, polar and thermally labile biomolecules. Read More

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http://dx.doi.org/10.2174/157341109788680291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583570PMC
July 2009
10 Reads

Recent Advances in Anthocyanin Analysis and Characterization.

Curr Anal Chem 2008 Apr;4(2):75-101

Department of Medicinal Chemistry, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, New Jersey 08854.

Anthocyanins are a class of polyphenols responsible for the orange, red, purple and blue colors of many fruits, vegetables, grains, flowers and other plants. Consumption of anthocyanins has been linked as protective agents against many chronic diseases and possesses strong antioxidant properties leading to a variety of health benefits. In this review, we examine the advances in the chemical profiling of natural anthocyanins in plant and biological matrices using various chromatographic separations (HPLC and CE) coupled with different detection systems (UV, MS and NMR). Read More

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http://dx.doi.org/10.2174/157341108784587795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2783603PMC
April 2008
12 Reads
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