1,009,339 results match your criteria Culture health & sexuality[Journal]


Long non-coding RNA THRIL promotes LPS-induced inflammatory injury by down-regulating microRNA-125b in ATDC5 cells.

Int Immunopharmacol 2018 Dec 3;66:354-361. Epub 2018 Dec 3.

Department of Orthopaedics, China-Japan Union Hospital of Jilin University, Changchun 130033, China. Electronic address:

Background: Osteoarthritis is an age-related disorder of bone-joint that causes pain and disability in middle and older people. This study aimed to investigate the potential effects of long non-coding RNA (lncRNA) THRIL on lipopolysaccharide (LPS)-induced osteoarthritis cell injury model (ATDC5 cell inflammatory injury), as well as the possible internal molecular mechanisms.

Methods: Cell viability and apoptosis were assessed using CCK-8 assay and Guava Nexin assay, respectively. Read More

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December 2018
2 Reads

Medium composition optimization, structural characterization, and antioxidant activity of exopolysaccharides from the medicinal mushroom Ganoderma lingzhi.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Beijing Advanced Innovation Centre for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China; Institute of Microbiology, Beijing Forestry University, Beijing 100083, China.

To contribute towards effective exploitation and utilization of natural antioxidants, response surface methodology (RSM) was employed to optimize the medium composition for the production of exopolysaccharides from the medicinal mushroom Ganoderma lingzhi (GLEPS). An optimal medium for GLEPS production was gave through Plackett-Burman design, path of steepest ascent, and Box-Behnken design as follows: glucose (59.62 g/L), yeast extract (10. Read More

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December 2018
1 Read

In vitro fermentation of O‑acetyl‑arabinoxylan from bamboo shavings by human colonic microbiota.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Institute of Agricultural Engineering, Fujian Academy of Agricultural Sciences, Fuzhou 350003, PR China.

BSH-1 is an O‑acetyl-arabinoxylan obtained from bamboo shavings. This study investigated its fermentation behavior by human colonic bacteria in vitro. Results showed that BSH-1 remarkably modulated the composition of colonic microbiota, mainly by increasing the growth of potential beneficial genera (i. Read More

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December 2018
1 Read

Influence of various uropathogens on crystallization of urine mineral components caused by Proteus mirabilis.

Res Microbiol 2018 Dec 3. Epub 2018 Dec 3.

Department of Biology of Bacteria, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland. Electronic address:

Infectious urolithiasis is a consequence of long-standing urinary tract infections with urease-positive bacteria, especially Proteus spp. However, because of the often mixed nature of urinary tract infections, in the case of urinary stones formation, several species of bacteria may be involved in the process. The purpose of the study was to determine the impact of the bacterial species: Escherichia coli, Klebsiella pneumoniae, Providencia stuartii, Pseudomonas aeruginosa and Staphylococcus aureus on the crystallization caused by Proteus mirabilis. Read More

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December 2018
1 Read

Identification of risk factors and diagnostic methodology in serositis by M. tuberculosis

Rev Med Inst Mex Seguro Soc 2018 11 30;56(4):364-370. Epub 2018 Nov 30.

Secretaría de Salud, Hospital General de México “Dr. Eduardo Liceaga”, Unidad de Neumología y Cirugía de Tórax. Ciudad de México, México

Background: Tuberculosis is a public health problem, extrapulmonary presentations have increased, it is difficult to diagnose because of the low bacillary load.

Objective: To identify risk factors and to evaluate the efficiency of diagnostic methods in pleural, meningeal, peritoneal and pericardial tuberculosis.

Methods: Prospective study of cases and controls. Read More

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November 2018

Epithelial-Mesenchymal Transdifferentiation in Pediatric Lens Epithelial Cells.

Invest Ophthalmol Vis Sci 2018 Dec;59(15):5785-5794

Department of Ophthalmology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.

Purpose: Posterior capsule opacification (PCO) is a complication after cataract surgery, particularly in children. Epithelial-mesenchymal transition (EMT) of lens epithelial cells, mediated by transforming growth factor beta (TGFβ), contributes to PCO. However, its pathogenesis in children is poorly understood. Read More

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December 2018
2 Reads

Modelling Toxoplasma gondii infection in a 3D cell culture system In Vitro: Comparison with infection in 2D cell monolayers.

PLoS One 2018 6;13(12):e0208558. Epub 2018 Dec 6.

Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, United States of America.

Three-dimensional (3D) cell culture models bridge the gap between two-dimensional (2D) monolayer cultures and animal models. Physiologically relevant, 3D culture models have significantly advanced basic cell science and provide unique insights into host-pathogen interactions intrinsically linked to cell morphology. Toxoplasma gondii is an obligate intravacuolar parasite that chronically infects a large portion of the global human population. Read More

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December 2018

The multiple maternal legacy of the Late Iron Age group of Urville-Nacqueville (France, Normandy) documents a long-standing genetic contact zone in northwestern France.

PLoS One 2018 6;13(12):e0207459. Epub 2018 Dec 6.

De la Préhistoire à l'Actuel, Culture, Environnement, Anthropologie-UMR 5199, CNRS, Université de Bordeaux, Allée Geoffroy Saint-Hilaire, CS, Pessac Cedex, France.

The compilation of archaeological and genetic data for ancient European human groups has provided persuasive evidence for a complex series of migrations, population replacements and admixture until the Bronze Age. If the Bronze-to-Iron Age transition has been well documented archaeologically, ancient DNA (aDNA) remains rare for the latter period and does not precisely reflect the genetic diversity of European Celtic groups. In order to document the evolution of European communities, we analysed 45 individuals from the Late Iron Age (La Tène) Urville-Nacqueville necropolis in northwestern France, a region recognized as a major cultural contact zone between groups from both sides of the Channel. Read More

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December 2018

[Chronic Pain After Outpatient Inguinal Hernioplasty: Retrospective Cohort Study].

Acta Med Port 2018 Nov 30;31(11):624-632. Epub 2018 Nov 30.

Unidade da Dor. Serviço de Anestesiologia. Hospital de Santo António. Centro Hospitalar do Porto. Porto. Portugal.

Introduction: Chronic postoperative pain is the most frequent late complication of inguinal hernia repair surgery. The aim of this study is to evaluate the incidence of chronic post-hernioplasty pain in outpatient care at Centro Hospitalar do Porto, describe it, analyse its relation with other variables defined in the literature and study its functional interference.

Material And Methods: We performed a retrospective cohort study between February and May 2016, using a structured telephone interview composed of questions from the authors and sections of published questionnaires, two of which are validated for the Portuguese language and culture. Read More

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November 2018
1 Read

Microgravity-induced hepatogenic differentiation of rBMSCs on board the SJ-10 satellite.

FASEB J 2018 Dec 6:fj201802075R. Epub 2018 Dec 6.

Key Laboratory of Microgravity, Center of Biomechanics and Bioengineering, Institute of Mechanics, Chinese Academy of Sciences, Beijing, China.

Bone marrow-derived mesenchymal stem cells (BMSCs) are able to differentiate into functional hepatocytelike cells, which are expected to serve as a potential cell source in regenerative medicine, tissue engineering, and clinical treatment of liver injury. Little is known about whether and how space microgravity is able to direct the hepatogenic differentiation of BMSCs in the actual space microenvironment. In this study, we examined the effects of space microgravity on BMSC hepatogenic differentiation on board the SJ-10 Recoverable Scientific Satellite. Read More

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December 2018
1 Read
5.043 Impact Factor

MMP14 empowers tumor-initiating breast cancer cells under hypoxic nutrient-depleted conditions.

FASEB J 2018 Dec 6:fj201801127R. Epub 2018 Dec 6.

Institute of Molecular Medicine and Cell Research, Medical Faculty, University of Freiburg, Freiburg, Germany.

Tumor-initiating cells (TICs) existing in breast cancer are thought to be involved in initiation, progression, and relapse of tumors. In these processes, the epithelial-to-mesenchymal transition (EMT) and proteases are crucial factors that also dependent on the tumor milieu, including hypoxic nutrient-deprived, as well as normoxic nutrient-rich, environments. Therefore, we investigated EMT and proteases in TICs and their response to different environments by means of a newly generated immortalized TIC (iTIC) line. Read More

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December 2018
1 Read

Elevated truncated oxidized phospholipids as a factor exacerbating ALI in the aging lungs.

FASEB J 2018 Dec 6:fj201800981R. Epub 2018 Dec 6.

Department of Anesthesiology, University of Maryland School of Medicine, Baltimore, Maryland, USA.

As mechanisms controlling redox homeostasis become impaired with aging, exaggerated oxidant stress may cause disproportional oxidation of cell membranes and circulating phospholipids (PLs), leading to the formation of truncated oxidized PL products (Tr-OxPLs), which exhibit deleterious effects. This study investigated the role of elevated Tr-OxPLs as a factor exacerbating inflammation and lung barrier dysfunction in an animal model of aging. Mass spectrometry analysis of Tr-OxPL species in young (2-4 mo) and aging (18-24 mo) mice revealed elevated basal levels of several products [1-palmitoyl-2-(5-oxovaleroyl)- sn-glycero-phosphocholine (POVPC), 1-palmitoyl-2-glutaroyl- sn-glycero-phosphocholine, lysophosphocholine, 1-palmitoyl-2-(9-oxo-nonanoyl)- sn-glycero-3-phosphocholine, 1-palmitoyl-2-azelaoyl- sn-glycero-3-phosphocholine, O-1-O-palmitoyl-2-O-(5,8-dioxo-8-hydroxy-6-octenoyl)-l-glycero-3-phosphocholine, and others] in the aged lungs. Read More

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December 2018
2 Reads

Comorbidities and antimicrobial resistance in urological outpatients with positive urine culture

Rev Med Inst Mex Seguro Soc 2018 11 30;56(4):347-353. Epub 2018 Nov 30.

Instituto Mexicano del Seguro Social, Hospital General de Zona No. 33, Departamento de Urología. Monterrey, Nuevo León, México

Background: The urinary tract infections are the third cause of infections in Mexico. The inappropriate use of antibiotic has generated the presence of multidrugresistant bacteria.

Objective: To identify the bacterial resistance patterns of the hospital and to detect the present comorbidities that can modify the evolution of urinary tract infection for proper empirical management. Read More

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November 2018
1 Read

Inhibition of PTEN activates bovine non-growing follicles in vitro but increases DNA damage and reduces DNA repair response.

Hum Reprod 2018 Dec 6. Epub 2018 Dec 6.

Institute of Cell Biology and Genes and Development Group, CDBS Hugh Robson Building, University of Edinburgh, Edinburgh, UK.

Study Question: Does ovarian follicle activation by phosphatase homologue of chromosome-10 (PTEN) inhibition affect DNA damage and repair in bovine oocytes and granulosa cells?

Summary Answer: PTEN inhibition promotes bovine non-growing follicle activation but results in increased DNA damage and impaired DNA repair capacity in ovarian follicles in vitro.

What Is Known Already: Inhibition of PTEN is known to activate primordial follicles but may compromise further developmental potential. In breast cancer cells, PTEN inhibition represses nuclear translocation of breast cancer susceptibility 1 (BRCA1) and Rad51; this impairs DNA repair resulting in an accumulation of damaged DNA, which contributes to cell senescence. Read More

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December 2018

Luteal granulosa cells from natural cycles are more capable of maintaining their viability, steroidogenic activity and LH receptor expression than those of stimulated IVF cycles.

Hum Reprod 2018 Dec 6. Epub 2018 Dec 6.

Graduate School of Health Sciences, Koc University, Davutpasa, Topkapi, Istanbul, Turkey.

Study Question: Are there any differences in the molecular characteristics of the luteal granulosa cells (GC) obtained from stimulated versus non-stimulated (natural) IVF cycles that may help explain the defective luteal phase in the former?

Summary Answer: Luteal GC of stimulated IVF cycles, particularly those of agonist-triggered antagonist cycles, are less viable ex vivo, express LH receptor and anti-apoptotic genes at lower levels, undergo apoptosis earlier and fail to maintain their estradiol (E2) and progesterone (P4) production in comparison to natural cycle GC.

What Is Known Already: Luteal function is defective in stimulated IVF cycles, which necessitates P4 and/or hCG administration (known as luteal phase support) in order to improve clinical pregnancy rates and prevent miscarriage. The luteal phase becomes shorter and menstruation begins earlier than a natural cycle if a pregnancy cannot be achieved, indicative of early demise of corpus luteum (premature luteolysis). Read More

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December 2018

Associations Between Drosophila suzukii (Diptera: Drosophilidae) and Fungi in Raspberries.

Environ Entomol 2018 Dec 4. Epub 2018 Dec 4.

Department of Entomology, University of Maryland, College Park, MD.

The invasive vinegar fly, Drosophila suzukii Matsumura, has emerged as one of the most serious arthropod pests of primocane red raspberries (Rubus ideaus L.) in the United States. In raspberries, D. Read More

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December 2018

Novel Paranannizziopsis species in a Wagler's viper (Tropidolaemus wagleri), tentacled snakes (Erpeton tentaculatum), and a rhinoceros snake (Rhynchophis boulengeri) in a zoological collection.

Med Mycol 2018 Dec 6. Epub 2018 Dec 6.

Mycology Unit, Facultat de Medicina i Ciències de la Salut and IISPV, University Rovira i Virgili, Reus, Spain.

We report several cases of fungal infections in snakes associated with a new species within the genus Paranannizziopsis. Three juvenile Wagler's vipers (Tropidolaemus wagleri) presented with skin abnormalities or ulcerative dermatitis, and two snakes died. Histologic examination of skin from the living viper revealed hyperplastic, hyperkeratotic, and crusting epidermitis with intralesional fungal elements. Read More

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December 2018
3 Reads

Engineered Heart Slice Model of Arrhythmogenic Cardiomyopathy using Plakophilin-2 Mutant Myocytes.

Tissue Eng Part A 2018 Dec 6. Epub 2018 Dec 6.

Johns Hopkins University School of Medicine, Biomedical Engineering, Baltimore, Maryland, United States ;

Arrhythmogenic cardiomyopathy (AC), a cause of sudden cardiac death among young and otherwise healthy individuals, is a heritable disease that can be modeled in vitro using patient-specific cardiac myocytes (CMs) from induced pluripotent stem cells. An understanding of underlying disease mechanisms, particularly in the early concealed stages, could lead to new diagnosis and treatment strategies. However, multicellular syncytial models are needed to understand how genetically-encoded mutations of the desmosomes that interconnect cells lead to aberrant electrical conduction and arrhythmias. Read More

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December 2018

Enhanced intrinsic CYP3A4 activity in human hepatic C3A cells with optically controlled CRISPR/dCas9 activator complex.

Integr Biol (Camb) 2018 Dec 6. Epub 2018 Dec 6.

Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Haidian District, Beijing, 100191, China.

Human hepatic C3A cells have been applied in bioartificial liver development, although these cells display low intrinsic cytochrome P450 3A4 (CYP3A4) enzyme activity. We aimed to enhance CYP3A4 enzyme activity of C3A cells utilizing CRISPR gene editing technology. We designed two CYP3A4 expression enhanced systems applying clustered regularly interspaced short palindromic repeats (CRISPR) gene technology: a CRISPR-on activation system including dCas9-VP64-GFP and two U6-sgRNA-mCherry elements, and a light-controlled CRISPR-on activation system combining our CRISPR-on activation system with an optical control system to facilitate regulation of CYP3A4 expression for various applications. Read More

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December 2018
3 Reads

Bacterial detection and identification from human synovial fluids on an integrated microfluidic system.

Analyst 2018 Dec 6. Epub 2018 Dec 6.

Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan. and Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan and Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu, Taiwan.

Periprosthetic joint infections (PJIs) are among the most severe complications emerging from prosthetic joint replacement surgeries. In order to possess a rapid means of diagnosing PJIs, an integrated microfluidic system was developed herein for detecting and identifying bacteria in human synovial fluid (HSF). The entire molecular diagnostic process, including (1) sample treatment, (2) bacterial isolation, (3) bacterial lysis, (4) nucleic acid amplification (via polymerase chain reaction (PCR)), and (5) optical detection, could be automated on a single chip. Read More

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December 2018

Concurrent validity and reliability of proprietary and open-source jump mat systems for the assessment of vertical jumps in sport sciences.

Acta Bioeng Biomech 2018 ;20(4):51-57

Department of Sport Sciences, University of Alicante, Ctra. San Vicente, Alicante, Spain.

Purpose: Vertical jump tests are used to assess lower-limb power of athletes in sport sciences. Flight time measurement with jump-mat systems is the most common procedure for this purpose. The aim of this study was to analyze the concurrent validity and reliability of two proprietary systems (Globus and Axon) and an open-source system (Chronojump). Read More

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January 2018

Target discovery for new antitubercular drugs using a large dataset of growth inhibitors from PubChem.

Authors:
Robert C Goldman

Infect Disord Drug Targets 2018 Dec 5. Epub 2018 Dec 5.

Drug Discovery and Development, RCG Consulting, 16024 Golden Lakes Drive. United States.

The number of drugs available for treatment of active tuberculosis are diminishing due to increased multidrug resistance selection in Mycobacterium tuberculosis leading to multiple (MDR) and extensively (XDR) resistant strains. Also, TB is treated with multiple drugs to minimize further resistance development, mandating a sustained effort to identify new lead compounds for treating drug resistant TB, and shortening time to cure for all TB infections. High throughput screening, a well-known approach to discovery of new leads, is conducted in two basic modes 1) using whole cells and screening for inhibition of growth, or whole cell reporter cells that signals when a specific pathway is perturbed, and 2) in vitro non-cell based enzyme or other functional assays for direct ligand-target binding. Read More

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December 2018

Doxorubicin effects on leukemia and breast cancer cells in culture on the TK1 protein levels using AroCell TK 210 ELISA: a tool for drug development.

Nucleosides Nucleotides Nucleic Acids 2018 Dec 6:1-8. Epub 2018 Dec 6.

a Department of Anatomy, Physiology and Biochemistry , Swedish University of Agricultural Sciences , VHC , Uppsala , Sweden.

In this study changes in thymidine kinase 1 (TK1) levels after 24 hours of Doxorubicin (Dox) exposure of CEM and MDA MB-231 cells were determined using the commercially available AroCell TK 210 ELISA test. In cell extracts, TK1 levels increased twofold with 1 µM Dox in both cell lines, while the TK1 levels in the culture media increased with 5 and 10 µM of Dox only in case of CEM cells. In conclusion, this study reveals that the TK 210 ELISA can measure changes in intra- and extracellular TK1 levels apparently related to the mechanism of cytotoxicity of anti-cancer agents. Read More

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December 2018

Identifying Lysophosphatidic Acid Acyltransferase β (LPAAT-β) as the Target of a Nanomolar Angiogenesis Inhibitor from a Phenotypic Screen Using the Polypharmacology Browser PPB2.

ChemMedChem 2018 Dec 6. Epub 2018 Dec 6.

Department of Chemistry and Biochemistry, National Center of Competence in Research NCCR TransCure, University of Bern, Freiestrasse 3, 3012, Bern, Switzerland.

By screening a focused library of kinase inhibitor analogues in a phenotypic co-culture assay for angiogenesis inhibition, we identified an aminotriazine that acts as a cytostatic nanomolar inhibitor. However, this aminotriazine was found to be completely inactive in a whole-kinome profiling assay. To decipher its mechanism of action, we used the online target prediction tool PPB2 (http://ppb2. Read More

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December 2018

The antibiotic action of methylarsenite is an emergent property of microbial communities.

Mol Microbiol 2018 Nov 22. Epub 2018 Nov 22.

Department of Cellular Biology and Pharmacology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, 33199, USA.

Arsenic is the most ubiquitous environmental toxin. Here, we demonstrate that bacteria have evolved the ability to use arsenic to gain a competitive advantage over other bacteria at least twice. Microbes generate toxic methylarsenite (MAs(III)) by methylation of arsenite (As(III)) or reduction of methylarsenate (MAs(V)). Read More

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November 2018

Paracrine Factors Released by Osteoblasts Provide Strong Platelet Engraftment Properties.

Stem Cells 2018 Dec 5. Epub 2018 Dec 5.

Canadian Blood Services, Centre for Innovation, Ottawa, Ontario, Canada.

Ex vivo expansion of hematopoietic stem cells (HSC) and progenitors may one day overcome the slow platelet engraftment kinetics associated with umbilical cord blood (UCB) transplantation. Serum-free medium conditioned with osteoblasts (i.e. Read More

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December 2018
1 Read

Extending spin-symmetry projected coupled-cluster to large model spaces using an iterative null-space projection technique.

J Comput Chem 2019 Jan;40(1):265-278

Graduate School of Science, Technology, and Innovation, Kobe University, Kobe, Hyogo, 657-0025, Japan.

Recently, we introduced an orbital-invariant approximate coupled-cluster (CC) method in the spin-projection manifold. The multi-determinantal property of spin-projection means that the parametrization in the spin-extended CC (ECC) ansatz is nonorthogonal and overcomplete. Therefore, the linear dependencies must be removed by an orthogonalization procedure to obtain meaningful solutions. Read More

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January 2019
1 Read

Ex vivo treatment of prostate tumor tissue recapitulates in vivo therapy response.

Prostate 2018 Dec 5. Epub 2018 Dec 5.

Department of Molecular Genetics, Erasmus MC, Rotterdam, The Netherlands.

Background: In vitro models of prostate cancer (PCa) are not always reliable to evaluate anticancer treatment efficacy. This limitation may be overcome by using viable tumor slice material. Here we report on the establishment of an optimized ex vivo method to culture tissue slices from patient-derived xenografts (PDX) of prostate cancer (PCa), to assess responses to PCa treatments. Read More

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December 2018
1 Read

PREDECT Protocols for Complex 2D/3D Cultures.

Methods Mol Biol 2019 ;1888:1-20

Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach an der Riß, Germany.

PREDECT, a European IMI consortium, has assumed the task to generate robust 2D and 3D culture platforms. Protocols established for 2D and 3D monoculture and stromal coculture models of increasing complexity (spheroid, stirred-tank bioreactor, Matrigel- and collagen-embedded cultures) have been established between six laboratories within academia, biotech, and pharma. These models were tested using three tumor cell lines (MCF7, LNCaP, and NCI-H1437), covering three pathologies (breast, prostate, and lung), but should be readily transferable to other model systems. Read More

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January 2019

Exploration of a cultural-adaptation of the EQ-5D for Thai population: A "bolt-on" experiment.

Qual Life Res 2018 Dec 5. Epub 2018 Dec 5.

Division of Social and Administrative Pharmacy, Department of Pharmacy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayuthaya Rd., Rajathevi, Bangkok, 10400, Thailand.

Purpose: As the EQ-5D was developed in western countries with only five dimensions, it might be insensitive to non-western populations including Thai. This study examined the impact of adding two candidate dimensions, "interpersonal relationships (IR)" and "activities related to bending knees (AK)," to the EQ-5D questionnaire, and evaluated their psychometric properties in a Thai population sample.

Methods: Face-to-face interviews were conducted with 600 Thai. Read More

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December 2018
1 Read

Mild heat stress induces hormetic effects in protecting the primary culture of mouse prefrontal cerebrocortical neurons from neuropathological alterations.

IBRO Rep 2018 Dec 14;5:110-115. Epub 2018 Nov 14.

Cellular Stress Response Laboratory, Cell Biology Division, Department of Zoology, Shivaji University, Kolhapur, Maharashtra, 416 004, India.

Hormesis is a dose response phenomenon of cells and organisms to various types of stressors. Mild stress stimulates prosurvival pathways and makes the cells adaptive to stressful conditions. It is a widely used fundamental dose-response phenomenon in many biomedical and toxicological sciences, radiation biology, health science etc. Read More

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December 2018

Isolation of halophilic bacteria associated with saline and alkaline-sodic soils by culture dependent approach.

Heliyon 2018 Nov 22;4(11):e00954. Epub 2018 Nov 22.

Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas 800, 44270, Guadalajara, Jalisco, Mexico.

Cultivable halophilic microorganisms were isolated and identified from saline and alkaline-sodic soils: Cuatro Cienegas, Sayula and San Marcos lakes. Physicochemical characteristics of soils were determined to understand the relationship between those and the microorganisms isolated. The Cuatro Cienegas soils had a neutral pH, EC of 2. Read More

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November 2018

Increased sensitivity of microbiological testing of cornea organ culture medium by additional resin treatment.

BMJ Open Ophthalmol 2018 10;3(1):e000173. Epub 2018 Nov 10.

Institute for Transfusion Medicine, University Tissue Bank, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Objective: This validation study investigates the treatment of cornea organ culture medium (Modified Eagle Medium, Biochrom GmbH, Berlin, Germany) with RESEP, a new medical device for antibiotics removal, before microbiological testing with BACTEC blood culture bottles.

Methods And Analysis: 10-100 colony forming units of and were inoculated in 9mL of cornea organ culture medium. In group A, the medium was withdrawn with RESEP and treated for 20 min at room temperature, and then inoculated in BACTEC Plus Aerobic/F/Anaerobic/F blood culture bottles. Read More

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November 2018
2 Reads

Tri-culture system for pro-hapten sensitizer identification and potency classification.

Technology (Singap World Sci) 2018 Jun 29;6(2):67-74. Epub 2018 Jun 29.

Department of Biomedical Engineering, Rutgers, the State University of New Jersey, 599 Taylor Road, Piscataway, NJ 08854, USA.

Allergic contact dermatitis (ACD) is an inflammatory disease that impacts 15-20% of the general population and accurate screening methods for chemical risk assessment are needed. However, most approaches poorly predict pre- and pro-hapten sensitizers, which require abiotic or metabolic conversion prior to inducing sensitization. We developed a tri-culture system comprised of MUTZ-3-derived Langerhans cells, HaCaT keratinocytes, and primary dermal fibroblasts to mimic the cellular and metabolic environments of skin sensitization. Read More

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June 2018
2 Reads

Should We Always Use Antibiotics after Urodynamic Studies in High-Risk Patients?

Biomed Res Int 2018 5;2018:1607425. Epub 2018 Nov 5.

2nd Department of Gynaecology, Medical University of Lublin, Lublin, Poland.

Aim: The aim of this observational study was to evaluate the effectiveness of a phytotherapic drug (Canephron N) in preventing urinary tract infection (UTI) in high-risk women undergoing urodynamic studies (UDS).

Methods: The study protocol was approved by the local institutional ethical committee. Adult women with at least one risk factor for acquiring UTI (defined as: age over 70, elevated postvoid residual urine>100 ml, recurrent UTI, pelvic organ prolapse (POP) ≥II in POP-Q scale, and neurogenic bladder) had received after UDS either a single oral dose of fosfomycin trometamol (FT) (3 grams) or a phytodrug containing centaury herb, lovage root, and rosemary leaves (5 ml taken orally three times daily for one week). Read More

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November 2018
2 Reads

New Achievements in High-Pressure Processing to Preserve Human Milk Bioactivity.

Front Pediatr 2018 16;6:323. Epub 2018 Nov 16.

Neonatal and Intensive Care Department, University Hospital, Medical University of Warsaw, Warsaw, Poland.

High-pressure processing (HPP) is a non-thermal technology that is being increasingly applied in food industries worldwide. It was proposed that this method could be used as an alternative to holder pasteurization (HoP; 62.5°C, 30 min) in milk banks but its impact on the immunologic, enzymatic and hormonal components of human milk has not yet been evaluated in detail. Read More

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November 2018
1 Read

Composition and Comparison of the Ocular Surface Microbiome in Infants and Older Children.

Transl Vis Sci Technol 2018 Nov 30;7(6):16. Epub 2018 Nov 30.

Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.

Purpose: Unlike other microbiomes of the body, the composition of the ocular surface microbiome (OSM) in children has yet to be thoroughly explored. Our goal was to evaluate the OSM in young infants and compare its composition to older children using both culture dependent and independent methodologies to assess for differences with age.

Methods: Prospective, observational, cross-sectional study of children <18 years of age at a university-based institution. Read More

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November 2018
1 Read

A case of and infections after bronchial thermoplasty.

Respirol Case Rep 2019 Feb 28;7(2):e00392. Epub 2018 Nov 28.

Department of Respiratory Medicine National Center for Global Health and Medicine Tokyo Japan.

Bronchial thermoplasty (BT) is a bronchoscopic treatment for severe asthma. A 35-year-old woman with uncontrolled severe asthma despite maximal pharmacological treatment underwent BT and started coughing after the first procedure. One month later, during the second BT procedure, there were white ulcerous lesions on the right B9 bronchus. Read More

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February 2019

Bootstrapped Biocatalysis: Biofilm-Derived Materials as Reversibly Functionalizable Multienzyme Surfaces.

ChemCatChem 2017 Dec 2;9(23):4328-4333. Epub 2017 Aug 2.

Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115 (USA).

Cell-free biocatalysis systems offer many benefits for chemical manufacturing, but their widespread applicability is hindered by high costs associated with enzyme purification, modification, and immobilization on solid substrates, in addition to the cost of the material substrates themselves. Herein, we report a "bootstrapped" biocatalysis substrate material that is produced directly in bacterial culture and is derived from biofilm matrix proteins, which self-assemble into a nanofibrous mesh. We demonstrate that this material can simultaneously purify and immobilize multiple enzymes site specifically and directly from crude cell lysates by using a panel of genetically programmed, mutually orthogonal conjugation domains. Read More

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December 2017

Myotilin, a New Topotecan Resistant Protein in Ovarian Cancer Cell Lines.

J Cancer 2018 22;9(23):4413-4421. Epub 2018 Oct 22.

Department of Histology and Embryology, Poznan University of Medical Sciences, Poznań, Poland.

Low effectiveness of chemotherapy in ovarian cancer results from development of drug resistance during treatment. Topotecan (TOP) is a chemotherapeutic drug used in second-line chemotherapy of this cancer. Unfortunately, during treatment cancer can develop diverse cellular and tissue specific mechanisms of resistance to cytotoxic drugs. Read More

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October 2018

Corrigendum: A Comparison of Techniques for Collecting Skin Microbiome Samples: Swabbing Versus Tape-Stripping.

Front Microbiol 2018 20;9:2812. Epub 2018 Nov 20.

Wellness Promotion Science Center, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.

[This corrects the article DOI: 10.3389/fmicb.2018. Read More

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November 2018
1 Read

SuperPolymyxin™ Medium for the Screening of Colistin-Resistant Gram-Negative Bacteria in Stool Samples.

Front Microbiol 2018 21;9:2809. Epub 2018 Nov 21.

Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.

Colistin is one of the last resort antimicrobials for the treatment of infections caused by multidrug-resistant Gram-negative bacteria. After the emergence of transferable colistin resistance genes (-1-5), a reliable culture-based screening method to detect colonization with colistin-resistant Gram-negative bacteria (CRGN) is needed. The objective of this study was to test the performance of SuperPolymyxin™ medium to screen for CRGN in stool samples and to compare different methods for the confirmation of colistin resistance (e. Read More

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November 2018

Atypical Manifestation of Disseminated Sporotrichosis in an AIDS Patient.

Case Rep Dermatol 2018 Sep-Dec;10(3):231-237. Epub 2018 Oct 24.

Sector of Dermatology and Post-Graduation Course, HUCFF-UFRJ and School of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

Sporotrichosis is an infection caused by fungi of the complex. The clinical lymphocutaneous presentation corresponds to the great majority of cases. With the increased incidence of sporotrichosis in the State of Rio de Janeiro, Brazil, atypical clinical forms have been reported with increasing frequency, usually associated with immunosuppression. Read More

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October 2018
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Visualization of Exo- and Endocytosis of AMPA Receptors During Hippocampal Synaptic Plasticity Around Postsynaptic-Like Membrane Formed on Glass Surface.

Authors:
Tomoo Hirano

Front Cell Neurosci 2018 21;12:442. Epub 2018 Nov 21.

Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto, Japan.

Regulation of exo- and endocytosis of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptor (AMPAR) plays a critical role in the expression of synaptic plasticity such as long-term potentiation (LTP) and long-term depression (LTD) at excitatory central synapses. Enhanced AMPAR exocytosis or endocytosis has been suggested to contribute to LTP or LTD, respectively. However, several unsettled fundamental questions have remained about AMPAR exo- and endocytosis in the basal condition and during synaptic plasticity: (1) Does the size of each exo- or endocytosis event, and/or do the frequencies of these events change during LTP or LTD? If they change, what are the time courses of the respective changes? (2) Where does the exo- or endocytosis preferentially occur in each condition: inside or in the vicinity of postsynaptic membrane, or in the extrasynaptic membrane? (3) Do different types of AMPAR, such as GluA1 homo-tetramer, GluA1/2 hetero-tetramer and GluA2/3 hetero-tetramer, show distinct exo- and endocytosis changes? To address these questions, we developed new methods to observe individual events of AMPAR exo- or endocytosis with a high signal to noise (SN) ratio in a culture preparation using total internal reflection fluorescence microscopy (TIRFM). Read More

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November 2018
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Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells.

ScientificWorldJournal 2018 4;2018:5454367. Epub 2018 Nov 4.

Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St., Moscow 117198, Russia.

In current study we have shown the impact of deuterium content in growth medium on proliferation rate of human cultured adipose-derived stem cells (ADSC). ADSCs have also demonstrated morphological changes when cultured in deuterated growth medium: the cell cultures did not reach confluence but acquired polygonal morphology with pronounced stress fibers. At high deuterium concentrations the ADSCs population doubling time increased which indicated the cell cycle retardation and decrease of cell proliferation rate. Read More

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November 2018

Protective effects of a novel facial cream against environmental pollution: in vivo and in vitro assessment.

Clin Cosmet Investig Dermatol 2018 12;11:571-578. Epub 2018 Nov 12.

Innovation and Development, ISDIN SA, Barcelona, Spain,

Background: The effects of pollution on health have received increasing attention in recent years. Extrinsic skin aging occurs via multiple processes, and pollution is now recognized as a major component, causing increased pigmentation and wrinkles via oxidative mechanisms. We tested the antipollution efficacy of a cosmetic facial cream (FC) by assessing its effects on carbon particle adhesion to skin and on oxidative and inflammatory pathways in the skin. Read More

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November 2018
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Distribution and drug resistance of pathogenic bacteria in ventilator-associated pneumonia at a local hospital of North-eastern China.

Infect Drug Resist 2018 13;11:2249-2255. Epub 2018 Nov 13.

Department of Emergency, Shengjing Hospital Affiliated to China Medical University, Heping District, Shenyang, Liaoning Province 110004, People's Republic of China,

Purpose: To study the distribution of pathogenic bacteria in ventilator-associated pneumonia (VAP), and epidemiological characteristics of extended-spectrum β lactamase (ESBL)-producing bacteria.

Patients And Methods: Lower respiratory tract secretions from 156 patients with mechanical ventilation were collected using a protective specimen brush (PSB), with quantitative bacterial culture carried out and antibiotic sensitivity measured. ESBLs produced by Gram-negative bacilli were detected using the double disk diffusion method and monitored by plasmid profiles. Read More

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November 2018

First reported nosocomial outbreak of harboring and in a neonatal intensive care unit in Cairo, Egypt.

Infect Drug Resist 2018 8;11:2211-2217. Epub 2018 Nov 8.

Department of Microbiology, Faculty of Dentistry and Oral Medicine, Future University, Cairo, Egypt.

Introduction: is a significant hospital-acquired pathogen, and many outbreaks of infection have been reported in neonates. We report a sudden breakout of harboring the and metallo-β-lactamase (MBL) genes that occurred from March to August 2015 in the neonatal intensive care unit of Cairo University Hospital, Cairo, Egypt.

Methods: During the study period, 40 nonduplicate clinical isolates of were collected from blood culture samples. Read More

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November 2018

Bacterial profile, antibiotic resistance pattern and associated factors among cancer patients at University of Gondar Hospital, Northwest Ethiopia.

Infect Drug Resist 2018 8;11:2169-2178. Epub 2018 Nov 8.

Department of Medical Microbiology, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia,

Introduction: Cancer is alarmingly increased in developing countries like Ethiopia, where multidrug resistant bacterial infection is rampant. The aim of this study was to determine the bacterial profile, antimicrobial resistance pattern, and associated factors among cancer patients attending University of Gondar Hospital.

Methods: A consecutive 216 cancer patients were recruited from February to April, 2017. Read More

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November 2018
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