3,910 results match your criteria Cryobiology [Journal]


Antioxidants and improved regrowth procedure facilitated cryoconservation of Paphiopedilum insigne Wall. Ex. Lindl. - An Endangered Slipper Orchid.

Cryobiology 2019 Feb 15. Epub 2019 Feb 15.

Plant Biotechnology Laboratory, Department of Botany, Centre for Advanced Studies, North-Eastern Hill University, Shillong, 793022, Meghalaya, India. Electronic address:

Orchids and their sustainability are very important issues that need global conservation efforts. Paphiopedilum insigne (an endangered orchid), is one of the most excessively exploited species of orchids and is mentioned in the IUCN Red List and Appendix I of CITES. The prospect for conservation and commercialization of this species would be strengthened with the development of improved cryopreservation techniques. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.02.003DOI Listing
February 2019

Use of trehalose in the semen cryopreservation of Amazonian catfish Leiarius marmoratus.

Cryobiology 2019 Feb 4. Epub 2019 Feb 4.

ReproPel Research Group, Veterinary Faculty, Universidade Federal de Pelotas (UFPel), Brazil; RAC, Reprodução Animal Comparada, Instituto de Ciências Biológicas, Universidade Federal de Rio Grande (FURG), Brazil. Electronic address:

The current study assessed a semen cryopreservation protocol in the Amazonian catfish Leiarius marmoratus, a freshwater fish, of rheophilic behavior, and of great importance for Brazilian fish farming. Eight males (n = 8) were stripped and the semen was cryopreserved if total motility in fresh semen was higher than 80%. The external cryoprotectant Trehalose was then diluted in Beltsvile Thawing Solution (BTS) extender in the following concentrations: 50, 100, 150, and 200 mM. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.02.001DOI Listing
February 2019

Preservation of female genetic resources of common carp through oogonial stem cell manipulation.

Cryobiology 2019 Feb 1. Epub 2019 Feb 1.

University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zátiší 728/II, 389 25, Vodňany, Czech Republic. Electronic address:

Several experiments were conducted in order to develop an optimal protocol for slow-rate freezing (-1 °C/min) and short-term storage (-80 or 4 °C) of common carp ovarian tissue fragments with an emphasis on oogonial stem cells (OSCs). Dimethyl sulfoxide (MeSO) with concentration of 1.5 M was identified as the best cryoprotectant in comparison to propylene glycol and methanol. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.016DOI Listing
February 2019

Cryopreservation of cell line Paesun by a rapid cooling.

Cryobiology 2019 Jan 31. Epub 2019 Jan 31.

Tissue Culture Laboratory, Virus Research Institute, Pyongyang Medical College, KIM IL SUNG University, Pyongyang, Democratic People's Republic of Korea.

The purpose of the present study was to clarify the possibility of a rapid cryopreservation for cell line Paesun by cooling in the range of 30-40 °C/min to vapor phase of -120 ∼-140 °C before immersion into liquid phase of liquid nitrogen using 10% MeSO. After thawing, these cells were examined with assaying viability by trypan blue exclusion staining and survival by cloning in monolayer; the percentages of cell and colony recovery obtained in rapid cooling had a tendency to be lower than that by slow cooling of 1 °C/min but there were no significant differences between them. In addition, post-thaw cells were examined by assaying proliferation and susceptibility to virus lines; there were no significant differences between before and after cryopreservation. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.017DOI Listing
January 2019

PD-1 blockade enhances the anti-tumor immune response induced by cryoablation in a murine model of renal cell carcinoma.

Cryobiology 2019 Jan 31. Epub 2019 Jan 31.

Department of Urology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.015DOI Listing
January 2019
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First birth of a healthy infant following intra-cytoplasmic sperm injection using a new permeable cryoprotectant-free sperm vitrification protocol.

Cryobiology 2019 Jan 30. Epub 2019 Jan 30.

IVF Spain Alicante, Av. de Ansaldo, 13, 03540, Alicante, Spain.

Purpose: The purpose of this study is to present the first birth of healthy infant born following ICSI using the new permeable cryoprotectant-free sperm vitrification protocol Easy-Sperm.

Principal Results: A 39 years old woman and his 40 years old partner underwent egg donation treatment at IVF-Spain Alicante (Spain). Half of the mature oocytes obtained from a young and healthy donor were fertilized by ICSI, using slow-frozen spermatozoa and the other half with vitrified spermatozoa. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.014DOI Listing
January 2019

Circulating soluble intercellular adhesion molecule-1 (sICAM-1) after exercise-induced muscular damage: Does the use of whole-body cryostimulation influence its concentration in blood?

Cryobiology 2019 Jan 30. Epub 2019 Jan 30.

University of Poitiers, Laboratoire Mobilité Vieillissement Exercice (MOVE)-EA6314, Faculty of Sport Sciences, Poitiers, France. Electronic address:

As soluble intercellular adhesion molecule-1 (sICAM-1) was recently hypothesized to be a key player in the mechanisms involved in exercise-induced muscular damage (EIMD), we investigated its circulating concentration changes in athletes before and after EIMD with and without the use of whole-body cryostimulation (WBC; 3 min at -110 °C) at the exercise end and repeated once a day during 4 days. We previously characterized plasma specimens from 11 endurance athletes who performed twice (randomized crossover design) strenuous running leading to EIMD, followed by passive recovery or WBC. Muscle soreness and inflammatory response were observed in both cases but the use of WBC induced a significant reduction in these responses (PlosOne 2011; 6:e22748). Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.013DOI Listing
January 2019

Vitrification of murine mature metaphase II oocytes perturbs DNA methylation reprogramming during preimplantation embryo development.

Cryobiology 2019 Jan 29. Epub 2019 Jan 29.

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China. Electronic address:

Accurate reprogramming of DNA methylation occurring in preimplantation embryos is critical for normal development of both fetus and placenta. Environmental stresses imposed on oocytes usually cause the abnormal DNA methylation reprogramming of early embryos. However, whether oocyte vitrification alters the reprogramming of DNA methylation (5 mC) and its derivatives in mouse preimplantation embryo development remains largely unknown. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.012DOI Listing
January 2019
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1.587 Impact Factor

CT-guided percutaneous cryoablation combined with systemic chemotherapy for liver metastases from esophageal carcinoma: Initial experience.

Cryobiology 2019 Jan 28. Epub 2019 Jan 28.

Department of Interventional Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Huan Hu West Road, Tianjin, 300060, PR China; Key Laboratory of Cancer Prevention and Therapy, Tian Jin, Huan Hu West Road, Tianjin, 300060, PR China; Tianjin's Clinical Research Center for Cancer, Huan Hu West Road, Tianjin, 300060, PR China. Electronic address:

Objective: To explore the feasibility, safety and effectiveness of percutaneous cryoablation combined with systemic chemotherapy in the treatment of liver metastases from esophageal carcinoma (ECLM).

Materials And Methods: We retrospectively collected data of 16 patients who received CT-guided percutaneous cryoablation concurrent systemic chemotherapy for liver metastases after primary esophageal carcinoma resection. Functional Assessment of Cancer Therapy-General (FACT-G) was used for the assessment of quality of life (QOL), and overall survival (OS), progression-free survival (PFS) and complications were also evaluated. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.011DOI Listing
January 2019

A preliminary study on the use of jenny colostrum to improve quality in extenders for freezing donkey semen.

Cryobiology 2019 Jan 24. Epub 2019 Jan 24.

Department of Animal Pathology, Faculty of Veterinary Medicine, Instituto Agroalimentario de Aragón IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain. Electronic address:

Sperm quality in donkeys (Equus asinus) after freezing thawing is still considered lower than that from other animals, including horses. The aim of this study was to test a new freezing extender supplemented with jenny colostrum on donkey sperm. After thawing, we evaluated sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional integrity by HOS-test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.009DOI Listing
January 2019
1 Read

Production of mouse fetuses using spermatozoa exposed temporarily to high temperature or continuously to room temperature after freeze-drying in Na-free/K-rich EGTA buffer.

Cryobiology 2019 Jan 22. Epub 2019 Jan 22.

Department of Biological Sciences, Asahikawa Medical University, 2-1-1-1 Midorigaoka-higashi, Asahikawa, 078-8510, Japan. Electronic address:

Present study aimed to determine to what extent freeze-dried spermatozoa were able to withstand high-temperature conditions: transient increase in storage temperature and long-term exposure to room temperature. Mouse spermatozoa were freeze-dried in EGTA/Tris-HCl buffered solution alkalinized using KOH (K-ETBS, pH 7.7), and then stored for up to 7 months at 4 °C or 25 °C. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.010DOI Listing
January 2019

Cryopreservation of mammalian embryos: Derivation of a method.

Cryobiology 2019 Feb 21;86:1-9. Epub 2019 Jan 21.

Department of Biological Sciences, University of New Orleans, New Orleans, LA, 70148, USA. Electronic address:

In 1972, a procedure was derived to cryopreserve mouse embryos. Over the past four decades, this procedure has been adapted to freeze embryos of more than twenty-five mammalian species. Cryopreservation of embryos has become a routine procedure in both veterinary and human medicine, having been used to freeze millions of embryos of mice and cattle, and many hundreds of thousands of human embryos. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.007DOI Listing
February 2019

Cryopreservation of shoot tips of recalcitrant and tropical species: Advances and strategies.

Cryobiology 2019 Jan 21. Epub 2019 Jan 21.

US Dept. of Agriculture and Department of Horticulture, Oregon State University, Corvallis, OR, 97331, USA. Electronic address:

There is a pressing need for practical and successful conservation efforts to establish long-term germplasm collections of recalcitrant and tropical species, given the challenge and threat that these plants are facing. Cryopreservation is the only way of conserving some of these species, especially those with temperature or desiccation sensitive (recalcitrant) seeds. This review covers reports on cryopreservation studies of shoot tips (apical and axillary) of tropical and subtropical plants. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.008DOI Listing
January 2019

Metabolic reorganization in winter: Regulation of pyruvate dehydrogenase (PDH) during long-term freezing and anoxia.

Cryobiology 2019 Feb 10;86:10-18. Epub 2019 Jan 10.

Department of Biology, Carleton University, Ottawa, Ontario, Canada. Electronic address:

Wood frogs, Rana sylvatica, can undergo prolonged periods of whole body freezing during winter, locking as much as 65-70% of total body water into extracellular ice and imposing both anoxia and dehydration on their cells. Metabolic rate depression (MRD) is an adaptation used by R. sylvatica to survive these environmental stresses, where a finite amount of ATP generated through anaerobic metabolism is directed towards maintaining pro-survival functions, while most ATP-expensive cellular processes are temporarily reduced in function. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.006DOI Listing
February 2019
1 Read
1.587 Impact Factor

Isochoric conditions enable high subfreezing temperature pancreatic islet preservation without osmotic cryoprotective agents.

Cryobiology 2019 Feb 7;86:130-133. Epub 2019 Jan 7.

Department of Mechanical Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA. Electronic address:

While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day preservation at high subfreezing temperatures in an isochoric chamber without osmotic cryoprotective agents (CPA), highlights the potential of isochoric cryopreservation in an application of clinical value. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.003DOI Listing
February 2019
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Recovery and functionality of cryopreserved peripheral blood mononuclear cells using five different xeno-free cryoprotective solutions.

Cryobiology 2019 Feb 7;86:25-32. Epub 2019 Jan 7.

National-Local Associated Engineering Laboratory (Shenzhen) for Personalized Cellular Therapy, Nanshan, Shenzhen, Guangdong, China; Shenzhen Kenuo Medical Lab, Nanshan, Shenzhen, Guangdong, China. Electronic address:

In this study, we compared three commercially available and two widely used CPAs for their ability of cryopreserving PBMCs. Similar survival (81.0%) and recovery rate (73. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183059
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http://dx.doi.org/10.1016/j.cryobiol.2019.01.004DOI Listing
February 2019
7 Reads
1.587 Impact Factor

Evaluation of changes in selected skin parameters under the influence of extremely low temperature.

Cryobiology 2019 Feb 7;86:19-24. Epub 2019 Jan 7.

Faculty of Physiotherapy, University School of Physical Education, Wroclaw, Poland.

The aim of the work was to evaluate changes in selected skin parameters under the influence of low temperature. The tests were conducted on a group of 20 women using whole-body cryotherapy. The average age of participants was 58. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.005DOI Listing
February 2019
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Effect of different olive oil-derived antioxidants (hydroxytyrosol and 3,4-dihydroxyphenylglycol) on the quality of frozen-thawed ram sperm.

Cryobiology 2019 Feb 3;86:33-39. Epub 2019 Jan 3.

Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Cordoba, Cordoba, 14014, Spain. Electronic address:

The aim of the present study was to evaluate the effect of the addition of different concentrations of two olive oil-derived antioxidants, hydroxytyrosol (3,4-dihydroxyphenylethanol, HT) and 3,4-dihydroxyphenylglycol (DHPG), on ovine semen during the freezing-thawing process. Sperm was collected, pooled and diluted with commercial extenders and then divided into aliquots supplemented with different concentrations (10 μg/ml, 30 μg/ml, 50 μg/ml and 70 μg/ml) of HT, DHPG and a mixture (MIX) of both antioxidants. A control group, without antioxidant, was also prepared. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183058
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http://dx.doi.org/10.1016/j.cryobiol.2019.01.002DOI Listing
February 2019
4 Reads

Toxicity tests of cryoprotecting agents for Mytilus galloprovincialis (Lamark, 1819) early developmental stages.

Cryobiology 2019 Feb 3;86:40-46. Epub 2019 Jan 3.

Marine Biological Resources Functional Preservation Service, Estación de Ciencias Mariñas de Toralla, Universidade de Vigo, Illa de Toralla, 36331, Coruxo, Vigo, Spain. Electronic address:

Global aquaculture production of blue mussel has increased over last years. This work reaffirms the great potential of cryopreservation technique on mussel industry and overcome economic barriers a cause of a traditional and rudimentary management and continue growing. The aim of this work is to set some preliminary basis attending to toxicity of cryoprotecting agents (CPAs) on different development stages of Mytilus galloprovincialis as a start point to develop a stable cryopreservation protocol. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.001DOI Listing
February 2019
2 Reads

Effects of four disaccharides on nucleation and growth of ice crystals in concentrated glycerol aqueous solution.

Cryobiology 2019 Feb 28;86:47-51. Epub 2018 Dec 28.

Department of Refrigeration and Cryogenics Engineering, Hefei University of Technology, Hefei, 230009, Anhui, China.

Devitrification has been determined to be one of the major causes of cell death in cryopreservation by vitrification method. Reliable quantification of the nucleation and growth of ice crystals of devitrification is of great importance for the optimization of the vitrification solutions. In the present study, cryomicroscopy was used to investigate the nucleation and growth of ice crystals in concentrated glycerol aqueous solution (60 wt%) in the presence of sucrose, trehalose, maltose and lactose. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.12.006DOI Listing
February 2019
1 Read

Effects of different concentration and combinations of cryoprotectants on sperm quality, functional integrity in three Indian horse breeds.

Cryobiology 2019 Feb 18;86:52-57. Epub 2018 Dec 18.

ICAR-National Research Centre on Equines, Hisar, Haryana, 225001, India.

Traditionally Glycerol (Gly) is being used as major cryoprotectant and its toxicity could be a reason for the variation on stallion sperm freezability and fertility. In an effort to minimize Gly toxicity alternative cryoprotective agents like Di-methyl Formamide (DMF) have been investigated. The effect of the cryoprotectant and dose of cryoprotective agent varies from breed to breed and also from stallion to stallion within the same breed. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183026
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http://dx.doi.org/10.1016/j.cryobiol.2018.12.005DOI Listing
February 2019
6 Reads
1.587 Impact Factor

The ideal holding time for boar semen is 24 h at 17 °C prior to short-cryopreservation protocols.

Cryobiology 2019 Feb 14;86:58-64. Epub 2018 Dec 14.

Swine research center, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil. Electronic address:

Boar semen cannot be immediately cryopreserved, it need be hold at 17 °C prior to cryopreservation, holding time has been used to improve cryopreserved boar semen, since holding time allows a prolonged interaction between spermatozoa and seminal plasma components. However, until now only few periods of holding time have been studied, and boar semen had been held at 17 °C for 24 h to facilitate its manufacture. Thus, this experiment aims to study the effect several holding time (0, 4, 8, 12, 24, 28 and 32 h) on boar spermatozoa post-thawed (PT) characteristics. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183026
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http://dx.doi.org/10.1016/j.cryobiol.2018.12.004DOI Listing
February 2019
9 Reads

Micro-quantity straw as a carrier for cryopreservation of oligozoospermic semen samples: Effects of storage times and cryoprotectant.

Cryobiology 2019 Feb 8;86:65-70. Epub 2018 Dec 8.

Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Electronic address:

Application of an appropriate freezing carrier is crucial for improving post-thaw recovery of oligozoospermic samples. In this study, our purpose is developing a user-friendly, easy handling and close micro-quantity (MQ) straw along with different freezing media, for cryopreservation of oligozoospermic samples. Twenty oligozoospermic semen samples were collected and mixed with glycerol egg yolk citrate (GEYC) or Spermfreeze (SPF) medium. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183022
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http://dx.doi.org/10.1016/j.cryobiol.2018.12.003DOI Listing
February 2019
5 Reads

Cryoprotectant toxicity in Caenorhabditis elegans.

Cryobiology 2019 Feb 4;86:71-76. Epub 2018 Dec 4.

Institute for Behavioral Genetics, University of Colorado, Boulder, USA; Department of Integrative Physiology, University of Colorado, Boulder, USA. Electronic address:

We have looked at the effects of the cryoprotectant M22 upon viability in the model organism C. elegans. M22 is a well-known vitrification solution which has been successfully used in the laboratory to preserve organs destined for transplantation. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.12.002DOI Listing
February 2019
1 Read

Single layer colloid centrifugation technique improves motility, viability and chromatin integrity of ram spermatozoa after thawing.

Cryobiology 2019 Feb 4;86:77-83. Epub 2018 Dec 4.

Clinic for Reproduction and Large Animals, University of Ljubljana, Vet Faculty, Ljubljana, Slovenia.

The cell membrane of ram spermatozoa is more sensitive to the freezing process than in other species due to its composition. As a result, the quality and viability of frozen thawed ram spermatozoa are often poor, which together with the specific structure of the ewe's cervix are the main reasons for lower fertility in ewes after intracervical insemination. In the present study we investigated the effects of semen centrifugation through a single layer of a species-specific colloid (Androcoll-O) on post-thaw quality of ram spermatozoa. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.12.001DOI Listing
February 2019
3 Reads

Cryopreservation of embryos and larvae of the edible sea urchin loxechinus albus (Molina, 1782).

Cryobiology 2019 Feb 23;86:84-88. Epub 2018 Nov 23.

Cryopreservation Laboratory, Department of Marine Biology, Universidad Católica del Norte, Coquimbo, Chile.

The natural population of the edible red sea urchin, Loxechinus albus, is decreasing due to overfishing. The embryos and larvae of the species are highly useful for monitoring marine pollution, which makes it necessary to conserve gametes, embryos and larvae to facilitate their use in diverse areas of aquaculture and environmental quality monitoring. This need can be met by cryopreserving individuals representing the different developmental stages to provide an ongoing supply of genetic material of the species. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.11.005DOI Listing
February 2019
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Production of F0 mice from embryonic stem cells injected eight-cell stage embryos which stored at refrigeration temperature.

Cryobiology 2019 Feb 22;86:89-94. Epub 2018 Nov 22.

Chongqing Academy of Animal Sciences, Chongqing, 402460, China; Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing, 402460, China; Key Laboratory of Pig Industry Sciences, Chongqing, 402460, China. Electronic address:

At refrigeration temperature, mouse embryos can retain their developmental ability for a couple of days. Previous research reports have focused on the effect of cool temperature on the development of 2-cell stage embryos, morulae or blastocysts and determined that the embryo still has the ability to produce offspring after about 48 h storage at refrigeration temperature. Here we examined whether refrigeration temperature affects the development of the eight-cell stage and if the stored eight-cell stage embryo can still be used as a host embryo for ES cell injection. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.11.004DOI Listing
February 2019
1 Read
1.587 Impact Factor

Genetic suppression of cryoprotectant toxicity.

Cryobiology 2019 Feb 17;86:95-102. Epub 2018 Nov 17.

Institute for Behavioral Genetics, University of Colorado Boulder, USA; Department of Integrative Physiology, University of Colorado Boulder, USA. Electronic address:

We report here a new, unbiased forward genetic method that uses transposon-mediated mutagenesis to enable the identification of mutations that confer cryoprotectant toxicity resistance (CTR). Our method is to select for resistance to the toxic effects of M22, a much-studied whole-organ vitrification solution. We report finding and characterizing six mutants that are resistant to M22. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183026
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http://dx.doi.org/10.1016/j.cryobiol.2018.11.003DOI Listing
February 2019
12 Reads

Apatite nanoparticles mediate intracellular delivery of trehalose and increase survival of cryopreserved cells.

Cryobiology 2019 Feb 17;86:103-110. Epub 2018 Nov 17.

Department of Cardiovascular Surgery, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China. Electronic address:

Cryopreservation of tissue cells is an important method to maintain cell viability and cellular function. However, cell viability and function are less than ideal by conventional cell cryopreservation methods, which may result in apoptosis and necrosis of cells in cryopreservation. Trehalose plays a role in maintaining cell structure and protecting cells from stress. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183027
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http://dx.doi.org/10.1016/j.cryobiol.2018.11.002DOI Listing
February 2019
10 Reads

Psychrotolerant bacteria isolated from the leaf apoplast of cold-adapted wild plants improve the cold resistance of bean (Phaseolus vulgaris L.) under low temperature.

Cryobiology 2019 Feb 10;86:111-119. Epub 2018 Nov 10.

Department of Biology Faculty of Science, Atatürk University, 25240 Erzurum, Turkey. Electronic address:

We have isolated psychrotolerant bacteria from the leaf apoplast of cold-adapted wild plants and aimed to investigate their effect on the cold resistance of bean (Phaseolus vulgaris L.). Based on the findings of 16S rRNA gene sequence analysis, 20 isolates belonging to 5 bacteria species (Pseudomonas fragi, P. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.11.001DOI Listing
February 2019
2 Reads

Application of cryoablation to treat peritoneal carcinomatosis from gastric cancer in a rabbit model.

Cryobiology 2018 Dec 27;85:12-16. Epub 2018 Oct 27.

Department of General Surgery, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China. Electronic address:

Objectives: Peritoneal carcinomatosis is one of the causes of death in patients with advanced gastric cancer. We assumed that cryoablation could be applied as adjuvant therapy to control peritoneal carcinomatosis from gastric cancer.

Methods: We investigated the feasibility of cryoablation technique in rabbit model using a novel cryoablation balloon probe. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183011
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http://dx.doi.org/10.1016/j.cryobiol.2018.10.263DOI Listing
December 2018
8 Reads

Lycium barbarum polysaccharide improves the development of mouse oocytes vitrified at the germinal vesicle stage.

Cryobiology 2018 Dec 1;85:7-11. Epub 2018 Nov 1.

College of Basic Medical Science, Jiujiang University, Jiujiang, Jiangxi, 332000, China; Key Laboratory of System Bio-medicine of Jiangxi Province, Jiujiang University, Jiujiang, Jiangxi, 332000, China. Electronic address:

The cryopreservation of cumulus oocyte complexes (COCs) is an important process in reproductive medicine. However, this process slows both embryo and offspring development. In this study, the effects of maturation medium supplementation with lycium barbarum polysaccharide (LBP) on the developmental competence of vitrified-warmed mouse COCs and the possible mechanisms facilitating this process were investigated. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.265DOI Listing
December 2018
2 Reads

Dimethylacetamide and trehalose for ram semen cryopreservation.

Cryobiology 2018 Dec 2;85:1-6. Epub 2018 Nov 2.

School of Veterinary and Animal Science Medicine, Federal University of Bahia, Av. Adhemar de Barros, 500, Salvador, Bahia, 40170-110, Brazil.

Eighteen semen samples from nine Santa Ines rams were cryopreserved in order to study the cryoprotective capacity of dimethylacetamide (DMA) at two concentrations (3% and 6%), and its interaction with trehalose (TRE, 100 mOsmol). A further objective of this study was to evaluate in vivo fertility of ram semen cryopreserved with dimethylacetamide. The control treatment was an extender medium with 6% glycerol (GLY). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183026
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http://dx.doi.org/10.1016/j.cryobiol.2018.10.266DOI Listing
December 2018
15 Reads

Workshop report: Cryopreservation of aquatic biomedical models.

Cryobiology 2019 Feb 31;86:120-129. Epub 2018 Oct 31.

Aquatic Germplasm and Genetic Resources Center, Louisiana State University Agricultural Center (LSUAC), Baton Rouge, LA, USA.

The genetic resources of aquatic biomedical model organisms are the products of millions of years of evolution, decades of scientific development, and hundreds of millions of dollars of research funding investment. Genetic resources (e.g. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.264DOI Listing
February 2019
10 Reads

Escherichia coli viability in an isochoric system at subfreezing temperatures.

Cryobiology 2018 Dec 23;85:17-24. Epub 2018 Oct 23.

Department of Mechanical Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA. Electronic address:

In comparison with isobaric (constant pressure) freezing, isochoric (constant volume) freezing reduces potential mechanical damage from ice crystals and exposes stored biological matter to a lower extracellular concentration, at the price of increased hydrostatic pressure. This study evaluates the effects of isochoric freezing to low temperatures and high pressures on Escherichia coli (E. coli) survival. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.262DOI Listing
December 2018
15 Reads

Optimization of protocols for microinjection-based delivery of cryoprotective agents into Japanese whiting Sillago japonica embryos.

Cryobiology 2018 Dec 9;85:25-32. Epub 2018 Oct 9.

Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo, 108-8477, Japan. Electronic address:

Microinjection has proven useful for introduction of low-permeability cryoprotective agents (CPAs) into fish eggs or embryos for cryopreservation. In this work, we examined the suitable conditions for single or combined microinjection into the perivitelline space (PS) and the yolk mass (YM) of embryos of the Japanese whiting, an alternative marine fish model for embryo cryopreservation studies. The parameters examined were injection volume, CPA type and concentration, vehicle (diluent), and suitable developmental stage. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183020
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http://dx.doi.org/10.1016/j.cryobiol.2018.10.007DOI Listing
December 2018
4 Reads

Thermal expansion of substrate may affect adhesion of Chinese hamster fibroblasts to surfaces during freezing.

Cryobiology 2019 Feb 9;86:134-139. Epub 2018 Oct 9.

Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada. Electronic address:

Despite success in cryopreservation of cells in suspension, cryopreservation of cells in monolayers is still challenging. One of the major problems is detachment of the cells from the substrate which occurs during cryopreservation. We hypothesized that this detachment may be due to a mismatch in the coefficient of linear thermal expansion α between glass and the frozen cell layer which manifests as residual stress and stress relaxation. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.006DOI Listing
February 2019
3 Reads

Properties of phase transition of ice binding protein from Arctic yeast (LeIBP) utilizing differential scanning calorimetry (DSC) and Raman spectroscopy.

Cryobiology 2018 Dec 5;85:33-38. Epub 2018 Oct 5.

Biomedical Engineering Research Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Republic of Korea; Department of Bionano Technology, Graduate School, Hanyang University, Seoul, 04763, Republic of Korea. Electronic address:

Ice binding proteins (IBPs) have been attracting significant interest on account of their characteristic of inhibiting ice growth and recrystallization. Owing to their unique characteristics, IBPs have been studied for applications in food, pharmaceuticals, and medicine, as well as from a general scientific point of view. In this study, we have used differential scanning calorimetry (DSC) and Raman spectroscopy as tools to understand the ice binding activity of the Arctic-yeast-originating extracellular ice binding glycoprotein (LeIBP) isolated from Leucosporidium sp. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.005DOI Listing
December 2018
5 Reads

Proteome variation of the rat liver after static cold storage assayed in an ex vivo model.

Cryobiology 2018 Dec 5;85:47-55. Epub 2018 Oct 5.

Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, 2000, Argentina. Electronic address:

Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.003DOI Listing
December 2018
16 Reads
1.590 Impact Factor

Hypothermic pulsatile perfusion of human pancreas: Preliminary technical feasibility study based on histology.

Cryobiology 2018 Dec 4;85:56-62. Epub 2018 Oct 4.

Institut de Transplantation Urologie Néphrologie (ITUN), CHU Nantes, Nantes, France.

Background: There are currently two approaches to hypothermic preservation for most solid organs: static or dynamic. Cold storage is the main method used for static storage (SS), while hypothermic pulsatile perfusion (HPP) and other machine perfusion-based methods, such as normothermic machine perfusion and oxygen persufflation, are the methods used for dynamic preservation. HPP is currently approved for kidney transplantation. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.002DOI Listing
December 2018
9 Reads

Cryopreservation of human umbilical vein and porcine corneal endothelial cell monolayers.

Cryobiology 2018 Dec 4;85:63-72. Epub 2018 Oct 4.

Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada; Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada. Electronic address:

Cryopreservation of endothelium is one of the major challenges in the cryopreservation of complex tissues. Human umbilical vein endothelial cells (HUVECs) in suspension are available commercially and recently their post-thaw cell membrane integrity was significantly improved by cryopreservation in 5% dimethyl sulfoxide (MeSO) and 6% hydroxyethyl starch (HES). However, cryopreservation of cells in monolayers has been elusive. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.001DOI Listing
December 2018
5 Reads

Post rapid freezing growth of Antarctic strain of Heterococcus sp. monitored by cell viability and chlorophyll fluorescence.

Cryobiology 2018 Dec 4;85:39-46. Epub 2018 Oct 4.

Department of Experimental Biology, Division of Plant Physiology and Anatomy, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic. Electronic address:

The soil microalgae of the genus Heterococcus are found in cold environments and have been reported for the terrestrial ecosystems of several Sub-Antarctic and Antarctic Islands. This study focused on resistance of Heterococcus sp. to sub-zero temperature. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.004DOI Listing
December 2018
1 Read

Impact of vitrification on granulosa cell survival and gene expression.

Cryobiology 2018 Dec 25;85:73-78. Epub 2018 Sep 25.

Medical University Vienna, Department of Obstetrics and Gynecology, Wien, Austria. Electronic address:

Introduction: Cryopreservation of ovarian tissue is an essential step in Ovarian Tissue Banking. In order to prevent the formation of ice crystals, typically the tissue is slowly frozen using a cryoprotectant. As an alternative the method of ultra-fast freezing by vitrification becomes more attention for freezing ovarian tissue because it has successfully been used for oocytes, embryos and sperm. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.09.006DOI Listing
December 2018
4 Reads

Changes of soluble sugars and ATP content during DMSO droplet freezing and PVS3 droplet vitrification of potato shoot tips.

Cryobiology 2018 Dec 23;85:79-86. Epub 2018 Sep 23.

Leibniz Institute of Plant Genetics and Crop Plant Research (IPK Gatersleben), Corrensstraße 3, 06466, Seeland, Germany. Electronic address:

The potato's great genetic diversity needs to be maintained for future agricultural applications and can be preserved at ultra-low temperatures. To decipher detailed physiological processes, the aim of the study was to analyze the regrowth in 28 gene bank accessions and to reveal metabolite changes in a subset of four accessions that showed pronounced differences after shoot tip cryopreservation using DMSO droplet freezing and PVS3 droplet vitrification. Regrowth varied in all 28 genotypes ranging from 5% ('Kagiri') to 100% ('Karakter') and was higher after PVS3 droplet vitrification (71 ± 19%) than after cryopreservation using DMSO (54 ± 17%). Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.09.005DOI Listing
December 2018
11 Reads

An improved method for vitrification of in vitro matured ovine oocytes; beneficial effects of Ethylene Glycol Tetraacetic acid, an intracellular calcium chelator.

Cryobiology 2018 Oct 3;84:82-90. Epub 2018 Jul 3.

Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.

Vitrification affects fertilization ability and developmental competence of mammalian oocytes. This effect may be more closely associated with an intracellular calcium rise induced by cryoprotectants. The present study aimed to assess whether addition of Ethylene Glycol Tetraacetic acid (EGTA) to vitrification solution could improve quality and developmental competence of in vitro matured ovine oocytes. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.07.001DOI Listing
October 2018
12 Reads
1.590 Impact Factor

Corrigendum to "Potassium ions in extender differentially influence the post-thaw sperm motility of salmonid fish" [Cryobiology 73(2) (2016) 248-256].

Cryobiology 2018 Oct;84:103

Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland.

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http://dx.doi.org/10.1016/j.cryobiol.2018.07.007DOI Listing
October 2018
12 Reads

The application of convolution neural network based cell segmentation during cryopreservation.

Cryobiology 2018 Dec 13;85:95-104. Epub 2018 Sep 13.

Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, China; Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei 230027, China. Electronic address:

For most of the cells, water permeability and plasma membrane properties play a vital role in the optimal protocol for successful cryopreservation. Measuring the water permeability of cells during subzero temperature is essential. So far, there is no perfect segmentation technique to be used for the image processing task on subzero temperature accurately. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183019
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http://dx.doi.org/10.1016/j.cryobiol.2018.09.003DOI Listing
December 2018
13 Reads

Faster, cheaper, defined and efficient vitrification for immature porcine oocytes through modification of exposure time, macromolecule source and temperature.

Cryobiology 2018 Dec 13;85:87-94. Epub 2018 Sep 13.

Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, 305-8602, Japan. Electronic address:

Vitrification reduces the developmental competence of porcine immature oocytes. We investigated the effects of modifying various factors on the viability and development of oocytes after vitrification. These factors included: 1) exposure to the vitrification solution, 2) macromolecule addition (bovine serum albumin (BSA) or polyvinyl pyrrolidone (PVP)), 3) treatment with cytochalasin B, 4) equilibration temperature, and 5) vitrification method (microdrop or Cryotop). Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.09.004DOI Listing
December 2018
2 Reads

Tolerance to vitrification of rat embryos at various developmental stages.

Cryobiology 2018 Oct 11;84:1-3. Epub 2018 Sep 11.

Division of Science and Engineering Graduate School of Arts and Science, Iwate University, Iwate, 020-8551, Japan; Department of Chemistry and Biological Sciences, Faculty of Science and Engineering, Iwate University, Iwate, 020-8551, Japan; Soft-Path Science and Engineering Research Center (SPERC), Iwate University, Iwate, 020-8551, Japan. Electronic address:

Numerous genetically engineered rat strains have been produced via genome editing. Although freezing of embryos is helpful for the production and storage of these valuable strains, the tolerance to freezing of embryos varies at each developmental stage of the embryo. This study examined the tolerance to freezing of rat embryos at various developmental stages, particularly at the pronuclear stage. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.09.002DOI Listing
October 2018
2 Reads