3,888 results match your criteria Cryobiology [Journal]


The ideal holding time for boar semen is 24 h at 17 °C prior to shot-cryopreservation protocols.

Cryobiology 2018 Dec 14. Epub 2018 Dec 14.

Swine research center, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil. Electronic address:

Boar semen cannot be immediately cryopreserved, it need be hold at 17 °C prior to cryopreservation, holding time has been used to improve cryopreserved boar semen, since holding time allows a prolonged interaction between spermatozoa and seminal plasma components. However, until now only few periods of holding time have been studied, and boar semen had been held at 17 °C for 24 h to facilitate its manufacture. Thus, this experiment aims to study the effect several holding time (0, 4, 8, 12, 24, 28 and 32 h) on boar spermatozoa post-thawed (PT) characteristics. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.12.004DOI Listing
December 2018

Micro-quantity straw as a carrier for cryopreservation of oligozoospermic semen samples: Effects of storage times and cryoprotectant.

Cryobiology 2018 Dec 8. Epub 2018 Dec 8.

Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Electronic address:

Application of an appropriate freezing carrier is crucial for improving post-thaw recovery of oligozoospermic samples. In this study, our purpose is developing a user-friendly, easy handling and close micro-quantity (MQ) straw along with different freezing media, for cryopreservation of oligozoospermic samples. Twenty oligozoospermic semen samples were collected and mixed with glycerol egg yolk citrate (GEYC) or Spermfreeze (SPF) medium. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183022
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http://dx.doi.org/10.1016/j.cryobiol.2018.12.003DOI Listing
December 2018
1 Read

Cryoprotectant toxicity in Caenorhabditis elegans.

Cryobiology 2018 Dec 4. Epub 2018 Dec 4.

Institute for Behavioral Genetics, University of Colorado, Boulder, USA; Department of Integrative Physiology, University of Colorado, Boulder, USA. Electronic address:

We have looked at the effects of the cryoprotectant M22 upon viability in the model organism C. elegans. M22 is a well-known vitrification solution which has been successfully used in the laboratory to preserve organs destined for transplantation. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.12.002DOI Listing
December 2018

Single layer colloid centrifugation technique improves motility, viability and chromatin integrity of ram spermatozoa after thawing.

Cryobiology 2018 Dec 4. Epub 2018 Dec 4.

Clinic for Reproduction and Large Animals, University of Ljubljana, Vet Faculty, Ljubljana, Slovenia.

The cell membrane of ram spermatozoa is more sensitive to the freezing process than in other species due to its composition. As a result, the quality and viability of frozen thawed ram spermatozoa are often poor, which together with the specific structure of the ewe's cervix are the main reasons for lower fertility in ewes after intracervical insemination. In the present study we investigated the effects of semen centrifugation through a single layer of a species-specific colloid (Androcoll-O) on post-thaw quality of ram spermatozoa. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.12.001DOI Listing
December 2018
1 Read

Cryopreservation of embryos and larvae of the edible sea urchin loxechinus albus (Molina, 1782).

Cryobiology 2018 Nov 23. Epub 2018 Nov 23.

Cryopreservation Laboratory, Department of Marine Biology, Universidad Católica del Norte, Coquimbo, Chile.

The natural population of the edible red sea urchin, Loxechinus albus, is decreasing due to overfishing. The embryos and larvae of the species are highly useful for monitoring marine pollution, which makes it necessary to conserve gametes, embryos and larvae to facilitate their use in diverse areas of aquaculture and environmental quality monitoring. This need can be met by cryopreserving individuals representing the different developmental stages to provide an ongoing supply of genetic material of the species. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.11.005DOI Listing
November 2018

Production of F0 mice from embryonic stem cells injected eight-cell stage embryos which stored at refrigeration temperature.

Cryobiology 2018 Nov 22. Epub 2018 Nov 22.

Chongqing Academy of Animal Sciences, Chongqing, 402460, China; Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing, 402460, China; Key Laboratory of Pig Industry Sciences, Chongqing, 402460, China. Electronic address:

At refrigeration temperature, mouse embryos can retain their developmental ability for a couple of days. Previous research reports have focused on the effect of cool temperature on the development of 2-cell stage embryos, morulae or blastocysts and determined that the embryo still has the ability to produce offspring after about 48 h storage at refrigeration temperature. Here we examined whether refrigeration temperature affects the development of the eight-cell stage and if the stored eight-cell stage embryo can still be used as a host embryo for ES cell injection. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.11.004DOI Listing
November 2018

Genetic suppression of cryoprotectant toxicity.

Cryobiology 2018 Nov 17. Epub 2018 Nov 17.

Institute for Behavioral Genetics, University of Colorado Boulder, USA; Department of Integrative Physiology, University of Colorado Boulder, USA. Electronic address:

We report here a new, unbiased forward genetic method that uses transposon-mediated mutagenesis to enable the identification of mutations that confer cryoprotectant toxicity resistance (CTR). Our method is to select for resistance to the toxic effects of M22, a much-studied whole-organ vitrification solution. We report finding and characterizing six mutants that are resistant to M22. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183026
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http://dx.doi.org/10.1016/j.cryobiol.2018.11.003DOI Listing
November 2018
7 Reads

Apatite nanoparticles mediate intracellular delivery of trehalose and increase survival of cryopreserved cells.

Cryobiology 2018 Nov 17. Epub 2018 Nov 17.

Department of Cardiovascular Surgery, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China. Electronic address:

Cryopreservation of tissue cells is an important method to maintain cell viability and cellular function. However, cell viability and function are less than ideal by conventional cell cryopreservation methods, which may result in apoptosis and necrosis of cells in cryopreservation. Trehalose plays a role in maintaining cell structure and protecting cells from stress. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183027
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http://dx.doi.org/10.1016/j.cryobiol.2018.11.002DOI Listing
November 2018
4 Reads

Psychrotolerant bacteria isolated from the leaf apoplast of cold-adapted wild plants improve the cold resistance of bean (Phaseolus vulgaris L.) under low temperature.

Cryobiology 2018 Nov 10. Epub 2018 Nov 10.

Department of Biology Faculty of Science, Atatürk University, 25240 Erzurum, Turkey. Electronic address:

We have isolated psychrotolerant bacteria from the leaf apoplast of cold-adapted wild plants and aimed to investigate their effect on the cold resistance of bean (Phaseolus vulgaris L.). Based on the findings of 16S rRNA gene sequence analysis, 20 isolates belonging to 5 bacteria species (Pseudomonas fragi, P. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.11.001DOI Listing
November 2018

Application of cryoablation to treat peritoneal carcinomatosis from gastric cancer in a rabbit model.

Cryobiology 2018 Oct 27. Epub 2018 Oct 27.

Department of General Surgery, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China. Electronic address:

Objectives: Peritoneal carcinomatosis is one of the causes of death in patients with advanced gastric cancer. We assumed that cryoablation could be applied as adjuvant therapy to control peritoneal carcinomatosis from gastric cancer.

Methods: We investigated the feasibility of cryoablation technique in rabbit model using a novel cryoablation balloon probe. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183011
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http://dx.doi.org/10.1016/j.cryobiol.2018.10.263DOI Listing
October 2018
3 Reads

Lycium barbarum polysaccharide improves the development of mouse oocytes vitrified at the germinal vesicle stage.

Cryobiology 2018 Nov 1. Epub 2018 Nov 1.

College of Basic Medical Science, Jiujiang University, Jiujiang, Jiangxi, 332000, China; Key Laboratory of System Bio-medicine of Jiangxi Province, Jiujiang University, Jiujiang, Jiangxi, 332000, China. Electronic address:

The cryopreservation of cumulus oocyte complexes (COCs) is an important process in reproductive medicine. However, this process slows both embryo and offspring development. In this study, the effects of maturation medium supplementation with lycium barbarum polysaccharide (LBP) on the developmental competence of vitrified-warmed mouse COCs and the possible mechanisms facilitating this process were investigated. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.265DOI Listing
November 2018

Dimethylacetamide and trehalose for ram semen cryopreservation.

Cryobiology 2018 Nov 2. Epub 2018 Nov 2.

School of Veterinary and Animal Science Medicine, Federal University of Bahia, Av. Adhemar de Barros, 500, Salvador, Bahia, 40170-110, Brazil.

Eighteen semen samples from nine Santa Ines rams were cryopreserved in order to study the cryoprotective capacity of dimethylacetamide (DMA) at two concentrations (3% and 6%), and its interaction with trehalose (TRE, 100 mOsmol). A further objective of this study was to evaluate in vivo fertility of ram semen cryopreserved with dimethylacetamide. The control treatment was an extender medium with 6% glycerol (GLY). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183026
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http://dx.doi.org/10.1016/j.cryobiol.2018.10.266DOI Listing
November 2018
8 Reads

Workshop report: Cryopreservation of aquatic biomedical models.

Cryobiology 2018 Oct 31. Epub 2018 Oct 31.

Aquatic Germplasm and Genetic Resources Center, Louisiana State University Agricultural Center (LSUAC), Baton Rouge, LA, USA.

The genetic resources of aquatic biomedical model organisms are the products of millions of years of evolution, decades of scientific development, and hundreds of millions of dollars of research funding investment. Genetic resources (e.g. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.264DOI Listing
October 2018
3 Reads

Escherichia coli viability in an isochoric system at subfreezing temperatures.

Cryobiology 2018 Oct 23. Epub 2018 Oct 23.

Department of Mechanical Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA. Electronic address:

In comparison with isobaric (constant pressure) freezing, isochoric (constant volume) freezing reduces potential mechanical damage from ice crystals and exposes stored biological matter to a lower extracellular concentration, at the price of increased hydrostatic pressure. This study evaluates the effects of isochoric freezing to low temperatures and high pressures on Escherichia coli (E. coli) survival. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183025
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http://dx.doi.org/10.1016/j.cryobiol.2018.10.262DOI Listing
October 2018
10 Reads

Optimization of protocols for microinjection-based delivery of cryoprotective agents into Japanese whiting Sillago japonica embryos.

Cryobiology 2018 Oct 9. Epub 2018 Oct 9.

Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo, 108-8477, Japan. Electronic address:

Microinjection has proven useful for introduction of low-permeability cryoprotective agents (CPAs) into fish eggs or embryos for cryopreservation. In this work, we examined the suitable conditions for single or combined microinjection into the perivitelline space (PS) and the yolk mass (YM) of embryos of the Japanese whiting, an alternative marine fish model for embryo cryopreservation studies. The parameters examined were injection volume, CPA type and concentration, vehicle (diluent), and suitable developmental stage. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183020
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http://dx.doi.org/10.1016/j.cryobiol.2018.10.007DOI Listing
October 2018
1 Read

Thermal expansion of substrate may affect adhesion of Chinese hamster fibroblasts to surfaces during freezing.

Cryobiology 2018 Oct 9. Epub 2018 Oct 9.

Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada. Electronic address:

Despite success in cryopreservation of cells in suspension, cryopreservation of cells in monolayers is still challenging. One of the major problems is detachment of the cells from the substrate which occurs during cryopreservation. We hypothesized that this detachment may be due to a mismatch in the coefficient of linear thermal expansion α between glass and the frozen cell layer which manifests as residual stress and stress relaxation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183024
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http://dx.doi.org/10.1016/j.cryobiol.2018.10.006DOI Listing
October 2018
1 Read

Properties of phase transition of ice binding protein from Arctic yeast (LeIBP) utilizing differential scanning calorimetry (DSC) and Raman spectroscopy.

Cryobiology 2018 Oct 5. Epub 2018 Oct 5.

Biomedical Engineering Research Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Republic of Korea; Department of Bionano Technology, Graduate School, Hanyang University, Seoul, 04763, Republic of Korea. Electronic address:

Ice binding proteins (IBPs) have been attracting significant interest on account of their characteristic of inhibiting ice growth and recrystallization. Owing to their unique characteristics, IBPs have been studied for applications in food, pharmaceuticals, and medicine, as well as from a general scientific point of view. In this study, we have used differential scanning calorimetry (DSC) and Raman spectroscopy as tools to understand the ice binding activity of the Arctic-yeast-originating extracellular ice binding glycoprotein (LeIBP) isolated from Leucosporidium sp. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183015
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http://dx.doi.org/10.1016/j.cryobiol.2018.10.005DOI Listing
October 2018
1 Read

Proteome variation of the rat liver after static cold storage assayed in an ex vivo model.

Cryobiology 2018 Oct 5. Epub 2018 Oct 5.

Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, 2000, Argentina. Electronic address:

Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183017
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http://dx.doi.org/10.1016/j.cryobiol.2018.10.003DOI Listing
October 2018
7 Reads
1.590 Impact Factor

Hypothermic pulsatile perfusion of human pancreas: Preliminary technical feasibility study based on histology.

Cryobiology 2018 Oct 4. Epub 2018 Oct 4.

Institut de Transplantation Urologie Néphrologie (ITUN), CHU Nantes, Nantes, France.

Background: There are currently two approaches to hypothermic preservation for most solid organs: static or dynamic. Cold storage is the main method used for static storage (SS), while hypothermic pulsatile perfusion (HPP) and other machine perfusion-based methods, such as normothermic machine perfusion and oxygen persufflation, are the methods used for dynamic preservation. HPP is currently approved for kidney transplantation. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.002DOI Listing
October 2018
5 Reads

Cryopreservation of human umbilical vein and porcine corneal endothelial cell monolayers.

Cryobiology 2018 Oct 4. Epub 2018 Oct 4.

Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada; Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada. Electronic address:

Cryopreservation of endothelium is one of the major challenges in the cryopreservation of complex tissues. Human umbilical vein endothelial cells (HUVECs) in suspension are available commercially and recently their post-thaw cell membrane integrity was significantly improved by cryopreservation in 5% dimethyl sulfoxide (MeSO) and 6% hydroxyethyl starch (HES). However, cryopreservation of cells in monolayers has been elusive. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.001DOI Listing
October 2018
4 Reads

Post rapid freezing growth of Antarctic strain of Heterococcus sp. monitored by cell viability and chlorophyll fluorescence.

Cryobiology 2018 Oct 4. Epub 2018 Oct 4.

Department of Experimental Biology, Division of Plant Physiology and Anatomy, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic. Electronic address:

The soil microalgae of the genus Heterococcus are found in cold environments and have been reported for the terrestrial ecosystems of several Sub-Antarctic and Antarctic Islands. This study focused on resistance of Heterococcus sp. to sub-zero temperature. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.10.004DOI Listing
October 2018

Impact of vitrification on granulosa cell survival and gene expression.

Cryobiology 2018 Sep 25. Epub 2018 Sep 25.

Medical University Vienna, Department of Obstetrics and Gynecology, Wien, Austria. Electronic address:

Introduction: Cryopreservation of ovarian tissue is an essential step in Ovarian Tissue Banking. In order to prevent the formation of ice crystals, typically the tissue is slowly frozen using a cryoprotectant. As an alternative the method of ultra-fast freezing by vitrification becomes more attention for freezing ovarian tissue because it has successfully been used for oocytes, embryos and sperm. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183015
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http://dx.doi.org/10.1016/j.cryobiol.2018.09.006DOI Listing
September 2018
2 Reads

Changes of soluble sugars and ATP content during DMSO droplet freezing and PVS3 droplet vitrification of potato shoot tips.

Cryobiology 2018 Sep 23. Epub 2018 Sep 23.

Leibniz Institute of Plant Genetics and Crop Plant Research (IPK Gatersleben), Corrensstraße 3, 06466, Seeland, Germany. Electronic address:

The potato's great genetic diversity needs to be maintained for future agricultural applications and can be preserved at ultra-low temperatures. To decipher detailed physiological processes, the aim of the study was to analyze the regrowth in 28 gene bank accessions and to reveal metabolite changes in a subset of four accessions that showed pronounced differences after shoot tip cryopreservation using DMSO droplet freezing and PVS3 droplet vitrification. Regrowth varied in all 28 genotypes ranging from 5% ('Kagiri') to 100% ('Karakter') and was higher after PVS3 droplet vitrification (71 ± 19%) than after cryopreservation using DMSO (54 ± 17%). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183018
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http://dx.doi.org/10.1016/j.cryobiol.2018.09.005DOI Listing
September 2018
6 Reads

An improved method for vitrification of in vitro matured ovine oocytes; beneficial effects of Ethylene Glycol Tetraacetic acid, an intracellular calcium chelator.

Cryobiology 2018 Oct 3;84:82-90. Epub 2018 Jul 3.

Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.

Vitrification affects fertilization ability and developmental competence of mammalian oocytes. This effect may be more closely associated with an intracellular calcium rise induced by cryoprotectants. The present study aimed to assess whether addition of Ethylene Glycol Tetraacetic acid (EGTA) to vitrification solution could improve quality and developmental competence of in vitro matured ovine oocytes. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183008
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http://dx.doi.org/10.1016/j.cryobiol.2018.07.001DOI Listing
October 2018
4 Reads
1.590 Impact Factor

Corrigendum to "Potassium ions in extender differentially influence the post-thaw sperm motility of salmonid fish" [Cryobiology 73(2) (2016) 248-256].

Cryobiology 2018 Oct;84:103

Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland.

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183024
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http://dx.doi.org/10.1016/j.cryobiol.2018.07.007DOI Listing
October 2018
10 Reads

The application of convolution neural network based cell segmentation during cryopreservation.

Cryobiology 2018 Sep 13. Epub 2018 Sep 13.

Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, China; Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei 230027, China. Electronic address:

For most of the cells, water permeability and plasma membrane properties play a vital role in the optimal protocol for successful cryopreservation. Measuring the water permeability of cells during subzero temperature is essential. So far, there is no perfect segmentation technique to be used for the image processing task on subzero temperature accurately. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183019
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http://dx.doi.org/10.1016/j.cryobiol.2018.09.003DOI Listing
September 2018
10 Reads

Faster, cheaper, defined and efficient vitrification for immature porcine oocytes through modification of exposure time, macromolecule source and temperature.

Cryobiology 2018 Sep 13. Epub 2018 Sep 13.

Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, 305-8602, Japan. Electronic address:

Vitrification reduces the developmental competence of porcine immature oocytes. We investigated the effects of modifying various factors on the viability and development of oocytes after vitrification. These factors included: 1) exposure to the vitrification solution, 2) macromolecule addition (bovine serum albumin (BSA) or polyvinyl pyrrolidone (PVP)), 3) treatment with cytochalasin B, 4) equilibration temperature, and 5) vitrification method (microdrop or Cryotop). Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.09.004DOI Listing
September 2018

Tolerance to vitrification of rat embryos at various developmental stages.

Cryobiology 2018 Oct 11;84:1-3. Epub 2018 Sep 11.

Division of Science and Engineering Graduate School of Arts and Science, Iwate University, Iwate, 020-8551, Japan; Department of Chemistry and Biological Sciences, Faculty of Science and Engineering, Iwate University, Iwate, 020-8551, Japan; Soft-Path Science and Engineering Research Center (SPERC), Iwate University, Iwate, 020-8551, Japan. Electronic address:

Numerous genetically engineered rat strains have been produced via genome editing. Although freezing of embryos is helpful for the production and storage of these valuable strains, the tolerance to freezing of embryos varies at each developmental stage of the embryo. This study examined the tolerance to freezing of rat embryos at various developmental stages, particularly at the pronuclear stage. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.09.002DOI Listing
October 2018
1 Read

Comparison of cryosurvival and spermatogenesis efficiency of cryopreserved neonatal mouse testicular tissue between three vitrification protocols and controlled-rate freezing.

Cryobiology 2018 Oct 6;84:4-9. Epub 2018 Sep 6.

Division of Urology, Mount Sinai Hospital, University of Toronto, Toronto, Ontario, Canada.

Grafting of cryopreserved testicular tissue is a promising tool for fertility and testicular function preservation in endangered species, mutant animals, or cancer patients for future use. In this study, we aimed to improve the whole neonatal mouse testicular tissue cryopreservation protocols by comparing cryosurvival, spermatogenesis, and androgen production of grafted testicular tissue after cryopreservation with three different vitrification protocols and an automated computed controlled-rate freezing. Whole neonatal mouse testes were vitrified with various vitrification solutions (V1) 40% EG + 18% Ficoll + 0. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.09.001DOI Listing
October 2018
8 Reads

Cryopreservation of Plagiognathops microlepis sperm.

Cryobiology 2018 Aug 27. Epub 2018 Aug 27.

Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University, Ningbo, Zhejiang, 315211, People's Republic of China. Electronic address:

Sperm was collected from cultured male fish and cryopreserved in 0.25 ml straws for the study of sperm cryopreservation. Different parameters were evaluated, including extender, dilution ratio, cryoprotectant type and concentration, equilibrium time, cooling height (in a two-step cooling protocol), and thawing temperature. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.08.012DOI Listing
August 2018
7 Reads

Construction of human artificial ovary from cryopreserved ovarian tissue: Appearance of apoptosis and necrosis after enzymatic isolation of follicles.

Cryobiology 2018 Oct 24;84:10-14. Epub 2018 Aug 24.

University Maternal Hospital, Department of Obstetrics and Gynaecology, Cologne University, Kerpener Str. 34, 50931, Cologne, Germany. Electronic address:

Earlier it was shown that number of retrieved follicles was significantly higher in Tumor Dissociation Enzyme (TDE)-treatment group compare to standard Liberase TM-group. The aim of our present investigations was to examine the effect of TDE on appearance of apoptosis and necrosis in follicles and stromal cells after digesting of cryopreserved ovarian cortex. Fresh and frozen ovarian cortex fragments (OCF) from 14 patients (29 ± 6 years old), sized 20-210 mm were randomly distributed into four treatment groups and digested with 16% TDE or 0. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.08.011DOI Listing
October 2018
7 Reads

Role of synthetic antifreeze agents in catalyzing ice nucleation.

Cryobiology 2018 Oct 22;84:91-94. Epub 2018 Aug 22.

The Center for Engineering in Medicine and BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, 02129, United States; Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, United States; Shriners Hospital for Children, Boston, MA, 02114, United States. Electronic address:

Nature endows antifreeze (glyco)proteins (AF(G)Ps) with the excellent capability of inhibiting ice crystal growth. Recent years have also witnessed the emergence of many potent AF(G)P mimics such as poly (vinyl alcohol) (PVA). As researchers are revealing the molecular mechanisms of inhibiting ice crystal growth by AF(G)Ps and their synthetic substitutes, there remains no agreement about their effect on ice nucleation. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.08.010DOI Listing
October 2018

Cryosurvival after exposure of IVF-derived Nellore embryos to different cryoprotectants and exposure times during vitrification.

Cryobiology 2018 Oct 18;84:95-97. Epub 2018 Aug 18.

Federal University of Tocantins, EMVZ- Campus, Araguaína, TO, Brazil.

We evaluated the effects of the vitrification solution (i.e., ethylene glycol (EG) + dimethyl sulfoxide (DMSO) with or without propylene glycol (PG)) and of exposure time on the re-expansion and hatching rates of vitrified Bos indicus embryos. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.08.009DOI Listing
October 2018

Dietary fish oil supplemented with vitamin E improves quality indicators of rooster cold-stored semen through reducing lipid peroxidation.

Cryobiology 2018 Oct 18;84:15-19. Epub 2018 Aug 18.

Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Cockerel semen is sensitive to cooling, which limits chilled storage of semen for more than 24 h. Results of artificial insemination with cold-stored semen are not desirable. This study was conducted to evaluate the effects of dietary fish oil and vitamin E (vitE) for cold-storage of rooster semen and its effects on parameters of semen during 48 h cooling preservation. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.08.008DOI Listing
October 2018
10 Reads

Convective heat transfer coefficients of open and closed Cryotop systems under different warming conditions.

Cryobiology 2018 Oct 14;84:20-26. Epub 2018 Aug 14.

Depto. de Ingeniería Química, Facultad de Ingeniería, Universidad Nacional de La Plata (Chemical Engineering Department, Faculty of Engineering, University of La Plata), Centro de Investigación y Desarrollo en Criotecnología de Alimentos (Center of Research and Development of Food Cryotechnology CIDCA, CONICET-UNLP-CIC PBA), Calle 47 y 116, La Plata, 1900, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (National Scientific and Technical Research Council, Argentina), Godoy Cruz 2290, CABA, 1425, Argentina.

The warming of cryopreserved samples supported by small volume devices is governed by heat transfer phenomena which are mathematically described by the solution of the transient heat conduction partial differential equations; the convective heat transfer coefficient (h) is an important parameter involved in the boundary condition which is related to the fluid dynamic behavior at the interface device-warming fluid (water, sucrose solution or air). Unfortunately, h values for small volume devices (i.e. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.08.007DOI Listing
October 2018

Effect of sericin supplementation on heat shock protein 70 (HSP70) expression, redox status and post thaw semen quality in goat.

Cryobiology 2018 Oct 10;84:33-39. Epub 2018 Aug 10.

Department of Veterinary Physiology, College of Veterinary Science and Animal Husbandry, Veterinary University, Mathura, Uttar Pradesh, India.

Cryopreservation results in substantial deterioration of heat shock protein 70 (HSP70) and ultra-structural changes in sperm organelles, resulting in a marked reduction in post-thaw semen quality. The present study was aimed to explicate the effect of sericin supplementation on expression profile of HSP70, redox status and post-thaw semen quality in Barbari goat. Five Barbari bucks were used to collect thirty semen ejaculates by using artificial vagina and each ejaculate was divided into three aliquots to which sericin was supplemented at 0% (Control), 0. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183014
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http://dx.doi.org/10.1016/j.cryobiol.2018.08.005DOI Listing
October 2018
7 Reads

Single blastocyst transfer (SET) and pregnancy outcome of day 5 and day 6 human blastocysts vitrified using a closed device.

Cryobiology 2018 Oct 10;84:40-45. Epub 2018 Aug 10.

Edinburgh Assisted Conception Programme, EFREC, Royal Infirmary of Edinburgh, 51 Little France Crescent, Old Dalkeith Road, Edinburgh, Scotland, EH164SA, UK.

This study investigates the utility of the Rapid-i closed device for vitrification of human blastocysts on day-5 (D5) and day-6 (D6) of development and the implantation and pregnancy rate following single blastocyst transfer (SBT) of warmed D5/D6 blastocysts. This retrospective cohort study was performed at Edinburgh Assisted Conception Programme, EFREC, Royal Infirmary of Edinburgh between January 2013 and January 2017. Good quality blastocysts were vitrified on D5 or D6 using Irvine Vitrification medium (Irvine Scientific-USA) and the Rapid-I closed Vitrification System™ (Vitrolife, Sweden). Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.08.004DOI Listing
October 2018

Lycopene and alpha-lipoic acid improve semen antioxidant enzymes activity and cashmere goat sperm function after cryopreservation.

Cryobiology 2018 Oct 10;84:27-32. Epub 2018 Aug 10.

College of Animal Science and Technology, Northwest A&F University, Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, Yangling, Shaanxi, 712100, PR China. Electronic address:

Cashmere goats, a unique biological resource in China, have the highest cashmere yield and best fiber quality in the world. The goal of this study was to determine the effects of cryopreserving with lycopene (LP) and alpha-lipoic acid (ALA) on the physiological characteristics of Cashmere goat spermatozoa. The results showed that sperm motility, acrosome integrity, membrane integrity, and mitochondrial activity of goat spermatozoa were greater in extenders containing 1. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.08.006DOI Listing
October 2018
5 Reads

Treatment with chemical delipidation forskolin prior to cryopreservation improves the survival rates of swamp buffalo (Bubalus bubalis) and bovine (Bos indicus) in vitro produced embryos.

Cryobiology 2018 Oct 7;84:46-51. Epub 2018 Aug 7.

Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok, 10330, Thailand. Electronic address:

The cryopreservation of embryos is a technology developed for long-term genetic preservation. However, high sensitivity to low temperatures due to a large number of intracellular lipids within ruminant embryos compromises the success of this technique. The aim of this study was to examine the effects of using of lipolytic chemical agent forskolin, during in vitro producing of buffalo and bovine embryos on lipid contents, cryotolerance and subsequent developmental competence of these embryos. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183021
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http://dx.doi.org/10.1016/j.cryobiol.2018.08.003DOI Listing
October 2018
15 Reads
1.590 Impact Factor

Virus infection reduces shoot proliferation of in vitro stock cultures and ability of cryopreserved shoot tips to regenerate into normal shoots in 'Gala' apple (Malus × domestica).

Cryobiology 2018 Oct 6;84:52-58. Epub 2018 Aug 6.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, PR China; College of Horticulture, Qingdao Agricultural University, Qingdao, 266109, Shandong, PR China. Electronic address:

Plant cryopreservation has provide secure back-ups of germplasm collections of vegetatively propagated crops. Often, recovery levels vary among laboratories when the same cryogenic procedures are used for the same genotypes. The present study investigated the effects of Apple stem grooving virus (ASGV) on shoot proliferation of in vitro stock cultures and recovery of cryopreserved shoot tips of 'Gala' apple. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.08.002DOI Listing
October 2018
2 Reads

Cryopreservation of red blood cells: Effect on rheologic properties and associated metabolic and nitric oxide related parameters.

Cryobiology 2018 Oct 3;84:59-68. Epub 2018 Aug 3.

Department of Molecular and Cellular Sports Medicine, German Sport University Cologne, Cologne, Germany. Electronic address:

Aim: High glycerol cryopreservation of red blood cells (RBCs) reduces metabolic processes at ultralow temperatures but less is known regarding the effect of cryopreservation on RBC nitric oxide (NO) metabolism, haemorheological properties, structural behaviour and membrane fragility.

Methods: Blood from ten healthy participants was sampled, glycerolized and stored at -80 °C (SB). Aliquots were thawed and further processed after 4, 8 and 12 weeks, respectively. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.08.001DOI Listing
October 2018

Creatine-loading preserves intestinal barrier function during organ preservation.

Cryobiology 2018 Oct 1;84:69-76. Epub 2018 Aug 1.

Department of Surgery, University of Alberta, Edmonton, Alberta, Canada. Electronic address:

We have developed a novel, intraluminal preservation solution that is tailored to the metabolic requirements of the intestine. This organ-specific solution addresses many of the problems associated with low temperature organ storage including energy, oxidative and osmotic stresses. However, conservation of energy levels remains one of the most difficult obstacles to overcome due to the inherent sensitivity of the mucosa to ischemia. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.07.009DOI Listing
October 2018

Defining the optimal cryoprotectant and concentration for cryopreservation of limbal stem cells.

Cryobiology 2018 Oct 31;84:98-102. Epub 2018 Jul 31.

Division of Ophthalmology, University of Bristol, Bristol Eye Hospital, Lower Maudlin Street, Bristol BS1 2LX, UK.

Limbal stem cell (LSC) deficiency causes progressive loss of vision but may be treated by transplant of autologous LSCs. Cryopreservation has the potential to indefinitely extend the lifespan of LSCs allowing re-transplant in case of graft failure. In this study, we aimed to identify the optimal cryoprotectant and cryoprotectant concentration for LSC cultures. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.07.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167250PMC
October 2018
2 Reads

Adaptation of the FK506 binding protein 1B to hibernation in bats.

Cryobiology 2018 Aug 6;83:1-8. Epub 2018 Jul 6.

Laboratory of Molecular Biology and Evolution, School of Life Science, East China Normal University, Shanghai, China. Electronic address:

Hibernation is an adaptive strategy used by some animals to cope with cold and food shortage. The heart rate, overall energy need, body temperature, and many other physiological functions are greatly reduced during torpor but promptly return to normal levels upon arousal. The heartbeat of torpid bats can be hundreds fold lower than that of active bats, indicating that hibernating bats have a remarkable ability to control excitation-contraction coupling in cardiac muscle. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.07.004DOI Listing
August 2018
7 Reads

Rapid cooling of rabbit embryos in a synthetic medium.

Cryobiology 2018 Jul 24. Epub 2018 Jul 24.

Université de Lyon, VetAgro Sup, Interaction cellule environnement, Marcy l'Etoile, France; Université de Lyon, ISARA-Lyon, Lyon, France.

Embryo cryopreservation media usually contain animal-derived products, such as bovine serum albumin (BSA). These products present two major disadvantages: an undefined variable composition and a risk of pathogen transmission. We aimed to evaluate the effect of replacing BSA in rabbit embryo rapid cooling "freezing" and warming media with a chemically defined medium with no animal-derived products: STEM ALPHA. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.07.006DOI Listing
July 2018
2 Reads

Minimum volume Spatula MVD vitrification method improves embryo survival compared to traditional slow freezing, both for in vivo and in vitro produced mice embryos.

Cryobiology 2018 Oct 21;84:77-81. Epub 2018 Jul 21.

Unidad de Animales Transgénicos y de Experimentación (UATE), Institut Pasteur de Montevideo, Mataojo 2020, CP 11400, Montevideo, Uruguay. Electronic address:

This study was conducted to compare the effect of minimum volume Spatula MVD vitrification (VIT) versus traditional slow freezing (SLF) of mouse embryos. A total of 2,617 8-cell in vivo derived and 2-cell in vitro produced B6D2 mouse embryos were subjected to freezing/thawing or vitrification/warming, while fresh embryos were used as control group. Embryo recovery, survival and development rate, pregnancy rate and offspring production were analyzed. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.07.005DOI Listing
October 2018
6 Reads

Cat ovarian follicle ultrastructure after cryopreservation with ethylene glycol and dimethyl sulfoxide.

Cryobiology 2018 Aug 4;83:9-14. Epub 2018 Jul 4.

Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, Distrito Federal, 70910-900, Brazil. Electronic address:

Ovarian tissue cryopreservation is a promising technique for fertility maintenance. The aim of this study was to compare the morphology of domestic cat ovarian follicles after tissue cryopreservation with ethylene glycol (EG) and dimethyl sulfoxide (MeSO). Ovaries from healthy adult cats undergoing elective ovariohysterectomy were used. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.07.003DOI Listing
August 2018
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Note on "Glass formation in plant anhydrobiotes: Survival in the dry state".

Authors:
Chu W Kwang-Hua

Cryobiology 2018 Aug 5;83:95-96. Epub 2018 Jul 5.

Distribution Centre, 2/F, No. 24, Lane 260, Section 1, Road Muzha, Taiwan, 116, Taipei, China; Transfer Centre, 4/F, 16, Lane 21, Road Kwang-Hui, Taiwan, 116, Taipei, China. Electronic address:

We make some crucial remarks about the recent review by Buitink and Leprince (2004) [Cryobiology 48 (2004) 215-228] considering their false definition about the glass transition temperature based on the value of (shear) viscosity: 10 Pa⋅s. The correct value is 10 Pa⋅s as evidenced from http://goldbook.iupac. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.07.002DOI Listing
August 2018
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A safety comparative study between freezing nitrogen ethanol composite and liquid nitrogen for cryotherapy of musculoskeletal tumors.

Cryobiology 2018 Aug 25;83:34-39. Epub 2018 Jun 25.

Department of Orthopaedics & Traumatology, Taipei Veterans General Hospital, Taiwan; Department of Orthopaedics, Therapeutical and Research Center of Musculoskeletal Tumor, Taipei Veterans General Hospital, Taiwan; Orthopaedic Department School of Medicine, National Yang-Ming University, Taiwan. Electronic address:

Freezing nitrogen ethanol composite (FNEC) showed effective cryoablative ability for bone tumor ex vivo and in vivo comparable to liquid nitrogen (LN). We therefore wished to compare the radiant cooling damage of the surrounding tissue between FNEC and LN. The evaluation of the radiant cooling damage was demonstrated human bone xenograft transplantation (HXT) in a mouse model. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.06.008DOI Listing
August 2018
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Natural deep eutectic systems as alternative nontoxic cryoprotective agents.

Cryobiology 2018 Aug 23;83:15-26. Epub 2018 Jun 23.

3B's Research Group - Biomaterials, Biodegradable and Biomimetic, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, 4805-017, Barco, Guimarães, Portugal; ICVS/3B's PT Government Associated Laboratory, 4710-243, Braga/Guimarães, Portugal; LAQV@REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal. Electronic address:

Natural deep eutectic systems (NADES) are mostly composed of natural primary metabolites such as sugars, sugar alcohols, organic acids, amino acids and amines. These simple molecules have been identified in animals living in environments with extreme temperature amplitudes, being responsible for their survival at negative temperatures during winter. Herein, we report for the first time the use of NADES based on trehalose (Treh) and glycerol (Gly) in cryopreservation, as cryoprotective agents (CPA). Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.06.010DOI Listing
August 2018
1 Read