3,928 results match your criteria Cryobiology [Journal]


Improved cryopreservation yield of pancreatic islets using combination of lower dose permeable cryoprotective agents.

Cryobiology 2019 Apr 13. Epub 2019 Apr 13.

Department of Surgery, University of California Irvine, Orange, CA, USA; Department of Biomedical Engineering, University of California Irvine, Irvine, CA, USA. Electronic address:

Islet transplantation has been shown to restore normoglycemia in animal models and for type 1 diabetic patients in clinical trials. One method of storing islets intended for transplantation is via cryobanking at very low temperatures (-196 °C). Cryobanking islets without the use of cryoprotecting agents (CPAs) contributes to cellular shear stress and cell death. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.04.004DOI Listing

Myocardial protection by propolis during prolonged hypothermic preservation.

Cryobiology 2019 Apr 11. Epub 2019 Apr 11.

U955-IMRB, équipe 03, Inserm, UPEC, Ecole Nationale Vétérinaire d'Alfort, Créteil, France.

Oxidative stress is involved in the pathogenesis of ischemia-reperfusion during myocardial transplantation. Therefore, graft preservation solutions may be improved by supplementation with antioxidants to minimize graft dysfunction caused by cold ischemic injury. Propolis is a polyphenol-rich substance which has an important antioxidant activity. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.04.003DOI Listing
April 2019
1 Read

Potent humanin analogue (HNG) protects human sperm from freeze-thaw-induced damage.

Cryobiology 2019 Apr 6. Epub 2019 Apr 6.

Department of Urology, The National Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, 445000, China. Electronic address:

This study was aimed to investigate the protective effect of potent humanin analogue (HNG) supplementation to freezing media on freezing-thawing induced human sperm damage. We collected semen samples with normal sperm parameters from 15 healthy men. After the swim-up processing, the motile spermatozoa from each of the men were allocated to four equal groups: In the control group, the spermatozoa were frozen in media without HNG supplementation. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.04.001DOI Listing
April 2019
1.587 Impact Factor

Cryopreservation of murine testicular Leydig cells by modified solid surface vitrification with supplementation of antioxidants.

Cryobiology 2019 Apr 5. Epub 2019 Apr 5.

Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha, 769008, India. Electronic address:

Reports on the vitrification of somatic cells are scarce. Here, we show that Leydig cells (murine cell line TM3) could be successfully vitrified by both open vitrification [plastic straw (PS) and plastic vials (PV)] and closed ultravitrification [microdrop (MD) and solid surface vitrification (SSV)], after protocol optimization. However, open ultravitrification resulted in better post-warming viability than closed systems of vitrification with highest success obtained in modified SSV (84. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183033
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http://dx.doi.org/10.1016/j.cryobiol.2019.04.002DOI Listing
April 2019
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Transcriptome and proteome analyses to investigate the molecular underpinnings of cold response in the Colorado potato beetle, Leptinotarsa decemlineata.

Cryobiology 2019 Apr 1. Epub 2019 Apr 1.

Department of Chemistry and Biochemistry, Université de Moncton, 18 Antonine-Maillet Avenue, Moncton, New Brunswick, E1A 3E9, Canada. Electronic address:

The Colorado potato beetle (Leptinotarsa decemlineata (Say)) is an insect that can cope with prolonged periods of low temperatures exposure. The molecular changes required to adapt to such conditions have not been thoroughly investigated in this insect. The current work aims at characterizing deregulated transcripts and proteins in adult L. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183065
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http://dx.doi.org/10.1016/j.cryobiol.2019.03.011DOI Listing
April 2019
3 Reads

Supplementary cryoprotective effect of carboxylated ε-poly-l-lysine during vitrification of rat pancreatic islets.

Cryobiology 2019 Mar 25. Epub 2019 Mar 25.

Graduate School of Science and Technology, Shinshu University, Ueda, Nagano, 386-8567, Japan; Graduate School of Medicine, Science and Technology, Shinshu University, Ueda, Nagano, 386-8567, Japan; Faculty of Textile Science and Technology, Shinshu University, Nagano, 386-8567, Japan. Electronic address:

This study was designed to investigate whether cryosurvival of rat pancreatic islets can be improved by carboxylated ε-poly-l-lysine (CPLL). Islets isolated from Wistar × Brown-Norway F1 rats (101-200 μm in diameter) were cryopreserved in three vitrification solutions containing ethylene glycol (EG; 30%, v/v) and CPLL (0%, 10%, or 20%, v/v) by Cryotop protocol (10 islets per device). The post-warm survival rate of the islets vitrified in the presence of 20% CPLL (74%), assessed by FDA/PI double staining, was higher than those in 0% and 10% CPLL (65% and 66%, respectively). Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.03.009DOI Listing

Three-dimensional printing can provide customizable probes for sensing and monitoring in cryobiology applications.

Cryobiology 2019 Mar 23. Epub 2019 Mar 23.

Aquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, LA, 70820, USA. Electronic address:

Cryopreservation has been recognized as a powerful tool for long-term preservation of genetic resources. However, the outcomes of cryopreservation by different user groups often vary due to inconsistency in procedures and freezing equipment. Herein, we report on the feasibility of providing customizable sensing probes with three-dimensional (3-D) printing to monitor cryopreservation phenomena. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.03.010DOI Listing

Effects of supplementation of Tris-egg yolk extender with royal jelly on chilled and frozen-thawed ram semen characteristics.

Cryobiology 2019 Mar 23. Epub 2019 Mar 23.

Clinic of Reproductive Medicine, Vetsuisse Faculty, University of Zurich, Switzerland.

This study was conducted to examine the effect of supplementation of Tris-egg yolk extender with lyophilized royal jelly (RJ) on chilled and frozen-thawed ram semen parameters. Ejaculates were collected by artificial vagina from 4 mature rams, twice a week for 4 weeks. Only samples with motility of ≥70% were included, pooled and divided into four equal parts and then diluted in extenders with various concentrations of RJ (0, 1, 3 and 5%, vol/vol) to a final concentration of 200 × 10 sperm/mL and was incubated at 37 °C for 30 min and were subsequently evaluated. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183030
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http://dx.doi.org/10.1016/j.cryobiol.2019.03.008DOI Listing
March 2019
5 Reads

Human oocyte morphometry before and after cryopreservation: A prospective cohort study.

Cryobiology 2019 Mar 21. Epub 2019 Mar 21.

Division of Human Reproduction, Hospital Das Clínicas, Department of Obstetrics and Gynecology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address:

The cryopreservation of human oocytes is an important strategy to spare fertility in women submitted to gonadotoxic therapy, ovarian surgery, or even to allow gestation by assisted reproduction technology after natural ovarian senescence. Methods to predict oocyte resistance to cryopreservation are still based on qualitative morphological assessment. In this study we evaluated whether morphometric characteristics of mature oocytes before vitrification and after warming are related to successful fertilization by intracytoplasmic sperm injection (ICSI). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240193006
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http://dx.doi.org/10.1016/j.cryobiol.2019.03.007DOI Listing
March 2019
8 Reads

Characterization of two copper/zinc superoxide dismutases (Cu/Zn-SODs) from the desert beetle Microdera punctipennis and their activities in protecting E. coli cells against cold.

Cryobiology 2019 Apr 16;87:15-27. Epub 2019 Mar 16.

Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 666 Shengli Road, Urumqi, 830046, China. Electronic address:

Superoxide dismutases (SODs) are crucial in scavenging reactive oxygen species (ROS); however, studies regarding SOD functions in insects under cold conditions are rare. In this paper, two novel Cu/Zn-SOD genes in the desert beetle Microdera punctipennis, an extracellular copper/zinc SOD (MpecCu/Zn-SOD) and an intracellular copper/zinc SOD (MpicCu/Zn-SOD), were identified and characterized. The results of quantitative real-time PCR showed that MpecCu/Zn-SOD expression was significantly up-regulated by 4 °C exposure for 0. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.03.006DOI Listing

Cryopreservation disrupts lipid rafts and heat shock proteins in yellow catfish sperm.

Cryobiology 2019 Apr 13;87:32-39. Epub 2019 Mar 13.

The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325035, PR China; Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, PR China. Electronic address:

Lipid rafts and associated membrane proteins (flotillin, caveolin) play important roles in cell signaling and sperm fertilization while heat shock proteins (Hsp) ensure properly protein folding to fulfill their physiological functions. The markedly reduced fertility in thawed sperm after cryopreservation could result from disrupted membrane lipid rafts and these proteins. To explore the effect of sperm cryopreservation on lipid rafts and heat shock proteins, we compared lipid raft integrity, and the expression levels of lipid raft associated proteins (Flot-1, Flot-2, Cav-1) as well as heat shock proteins (Hsp90, Hsp70) in fresh and thawed sperm cryopreserved under different scenarios in yellow catfish. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.03.004DOI Listing
April 2019
1 Read

Successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders.

Cryobiology 2019 Apr 13;87:28-31. Epub 2019 Mar 13.

Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa, 16059, Turkey.

The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thawing quality of drone sperm. Semen samples were collected from sexually mature drones. Pooled semen was diluted with extender without RJ (control) or supplemented with different concentrations of RJ (1, 2, 4 or 8%). Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.03.005DOI Listing

Negative air pressure treatment accelerates the penetration of permeable cryoprotectants into bovine ovarian tissue in vitrification protocol and improves cell density after vitrification.

Cryobiology 2019 Mar 9. Epub 2019 Mar 9.

Graduate School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, 252-5201, Japan. Electronic address:

Effects of additional physical treatments during vitrification of the bovine ovarian tissue were examined for increasing of permeability of ethylene glycol (EG) and dimethyl sulfoxide (Me2SO). The concentrations of EG and Me2SO and histological changes in the ovarian tissue were evaluated. In the first equilibration step (7. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.03.001DOI Listing

Improvement of rooster semen quality using coenzyme Q10 during cooling storage in the Lake extender.

Cryobiology 2019 Mar 8. Epub 2019 Mar 8.

Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

Sensitivity of rooster semen to stressful condition of cooling restricts the semen storage in commercial flocks for artificial insemination. This study was accomplished to investigate the effect of coenzyme Q10 (CoQ10) addition to the Lake extender during chilled-storage on the parameters of sperm quality and fertility performance. Roosters' pooled semen samples were assigned into equal parts and diluted with Lake extender supplemented with different concentrations of CoQ10 (0, 1, 2, 5 and 10 μM CoQ10). Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.03.003DOI Listing
March 2019
1 Read

Local cryostimulation acutely preserves maximum isometric handgrip strength following fatigue in young women.

Cryobiology 2019 Apr 9;87:40-46. Epub 2019 Mar 9.

Department of Biomedical Sciences for Health, Università Degli Studi di Milano, Milan, Italy; Metabolism Research Center, Endocrinology and Metabolism, IRCCS Policlinico San Donato, San Donato Milanese, Italy. Electronic address:

Several types of cryostimulation have been recently proposed to rapidly lower skin temperature therefore gaining a possible neuro/muscular recovery after strenuous exercise or, more generally, in sports. Local cryostimulation may be a viable and relatively portable tool to obtain physiological benefits in previously-efforted muscular districts. However, cohesive and standardized cryo-exposure protocols are lacking as well as the righteous procedure to efficaciously combine duration, treatments and temperature in relation to desirable effects on muscular strength. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.03.002DOI Listing

Does fennel extract ameliorate oxidative stress frozen-thawed ram sperm?

Cryobiology 2019 Apr 1;87:47-51. Epub 2019 Mar 1.

Department of Animal Science, University of Illinois, Urbana, IL, USA.

The aim of this study was to evaluate the quality of ram semen after cryopreservation with different levels of fennel (Foeniculum vulgare) extract (0 (F0), 5 (F5), 10 (F10) and 15 (F15) mg/L) and sperm concentrations (200 (C200) and 400 (C400) × 10 sperm/mL) in a soy lecithin (SL)-based extender. Twenty ejaculates were collected from four ghezel rams and diluted with eight sperm concentrations/fennel combinations: F0C200, F5C200, F10C200, F15C200, F0C400, F5C400, F10C400 and F15C400. Sperm motility, abnormality, plasma membrane, viability, mitochondrial activity, lipid peroxidation (LPO), mitochondrial activity and apoptotic changes were evaluated after freeze-thawing process. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.02.006DOI Listing
April 2019
1 Read
1.587 Impact Factor

Evaluation of five additives to mitigate toxicity of cryoprotective agents on porcine chondrocytes.

Cryobiology 2019 Feb 28. Epub 2019 Feb 28.

Department of Surgery, University of Alberta, Edmonton, Alberta, T6G 2B7, Canada. Electronic address:

Cryoprotective agents (CPAs) are used in cryopreservation protocols to achieve vitrification. However, the high CPA concentrations required to vitrify a tissue such as articular cartilage are a major drawback due to their cellular toxicity. Oxidation is one factor related to CPA toxicity to cells and tissues. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.02.004DOI Listing
February 2019
3 Reads

Vitrification induces critical subcellular damages in ram spermatozoa.

Cryobiology 2019 Apr 1;87:52-59. Epub 2019 Mar 1.

Department of Animal Medicine and Surgery, University of Cordoba, 14014, Cordoba, Spain. Electronic address:

The aim of the present study was to analyse morphological variations in ovine spermatozoa subjected to different cryopreservation protocols using high resolution imaging techniques. Ejaculates were pooled and diluted in Tris-based extender. Aliquots containing 300 × 10 spz/ml were prepared and evaluated a) after the semen collection and pooling, b) after conventional freezing, c) after vitrification of samples maintained at room temperature (22 °C) prior to vitrification, and d) after vitrification of samples maintained at 5 °C prior to vitrification. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.02.005DOI Listing
April 2019
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Antioxidants and improved regrowth procedure facilitated cryoconservation of Paphiopedilum insigne Wall. Ex. Lindl. - An Endangered Slipper Orchid.

Cryobiology 2019 Apr 15;87:60-67. Epub 2019 Feb 15.

Plant Biotechnology Laboratory, Department of Botany, Centre for Advanced Studies, North-Eastern Hill University, Shillong, 793022, Meghalaya, India. Electronic address:

Orchids and their sustainability are very important issues that need global conservation efforts. Paphiopedilum insigne (an endangered orchid), is one of the most excessively exploited species of orchids and is mentioned in the IUCN Red List and Appendix I of CITES. The prospect for conservation and commercialization of this species would be strengthened with the development of improved cryopreservation techniques. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183012
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http://dx.doi.org/10.1016/j.cryobiol.2019.02.003DOI Listing
April 2019
5 Reads

Use of trehalose in the semen cryopreservation of Amazonian catfish Leiarius marmoratus.

Cryobiology 2019 Apr 4;87:74-77. Epub 2019 Feb 4.

ReproPel Research Group, Veterinary Faculty, Universidade Federal de Pelotas (UFPel), Brazil; RAC, Reprodução Animal Comparada, Instituto de Ciências Biológicas, Universidade Federal de Rio Grande (FURG), Brazil. Electronic address:

The current study assessed a semen cryopreservation protocol in the Amazonian catfish Leiarius marmoratus, a freshwater fish, of rheophilic behavior, and of great importance for Brazilian fish farming. Eight males (n = 8) were stripped and the semen was cryopreserved if total motility in fresh semen was higher than 80%. The external cryoprotectant Trehalose was then diluted in Beltsvile Thawing Solution (BTS) extender in the following concentrations: 50, 100, 150, and 200 mM. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.02.001DOI Listing

Preservation of female genetic resources of common carp through oogonial stem cell manipulation.

Cryobiology 2019 Apr 1;87:78-85. Epub 2019 Feb 1.

University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zátiší 728/II, 389 25, Vodňany, Czech Republic. Electronic address:

Several experiments were conducted in order to develop an optimal protocol for slow-rate freezing (-1 °C/min) and short-term storage (-80 or 4 °C) of common carp ovarian tissue fragments with an emphasis on oogonial stem cells (OSCs). Dimethyl sulfoxide (MeSO) with concentration of 1.5 M was identified as the best cryoprotectant in comparison to propylene glycol and methanol. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.016DOI Listing

Cryopreservation of cell line Paesun by a rapid cooling.

Cryobiology 2019 Apr 31;87:115-116. Epub 2019 Jan 31.

Tissue Culture Laboratory, Virus Research Institute, Pyongyang Medical College, KIM IL SUNG University, Pyongyang, Democratic People's Republic of Korea.

The purpose of the present study was to clarify the possibility of a rapid cryopreservation for cell line Paesun by cooling in the range of 30-40 °C/min to vapor phase of -120 ∼-140 °C before immersion into liquid phase of liquid nitrogen using 10% MeSO. After thawing, these cells were examined with assaying viability by trypan blue exclusion staining and survival by cloning in monolayer; the percentages of cell and colony recovery obtained in rapid cooling had a tendency to be lower than that by slow cooling of 1 °C/min but there were no significant differences between them. In addition, post-thaw cells were examined by assaying proliferation and susceptibility to virus lines; there were no significant differences between before and after cryopreservation. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.017DOI Listing

PD-1 blockade enhances the anti-tumor immune response induced by cryoablation in a murine model of renal cell carcinoma.

Cryobiology 2019 Apr 31;87:86-90. Epub 2019 Jan 31.

Department of Urology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.015DOI Listing
April 2019
1 Read

First birth of a healthy infant following intra-cytoplasmic sperm injection using a new permeable cryoprotectant-free sperm vitrification protocol.

Cryobiology 2019 Apr 30;87:117-119. Epub 2019 Jan 30.

IVF Spain Alicante, Av. de Ansaldo, 13, 03540, Alicante, Spain.

Purpose: The purpose of this study is to present the first birth of healthy infant born following ICSI using the new permeable cryoprotectant-free sperm vitrification protocol Easy-Sperm.

Principal Results: A 39 years old woman and his 40 years old partner underwent egg donation treatment at IVF-Spain Alicante (Spain). Half of the mature oocytes obtained from a young and healthy donor were fertilized by ICSI, using slow-frozen spermatozoa and the other half with vitrified spermatozoa. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.014DOI Listing
April 2019
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Circulating soluble intercellular adhesion molecule-1 (sICAM-1) after exercise-induced muscular damage: Does the use of whole-body cryostimulation influence its concentration in blood?

Cryobiology 2019 Apr 30;87:120-122. Epub 2019 Jan 30.

University of Poitiers, Laboratoire Mobilité Vieillissement Exercice (MOVE)-EA6314, Faculty of Sport Sciences, Poitiers, France. Electronic address:

As soluble intercellular adhesion molecule-1 (sICAM-1) was recently hypothesized to be a key player in the mechanisms involved in exercise-induced muscular damage (EIMD), we investigated its circulating concentration changes in athletes before and after EIMD with and without the use of whole-body cryostimulation (WBC; 3 min at -110 °C) at the exercise end and repeated once a day during 4 days. We previously characterized plasma specimens from 11 endurance athletes who performed twice (randomized crossover design) strenuous running leading to EIMD, followed by passive recovery or WBC. Muscle soreness and inflammatory response were observed in both cases but the use of WBC induced a significant reduction in these responses (PlosOne 2011; 6:e22748). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183026
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http://dx.doi.org/10.1016/j.cryobiol.2019.01.013DOI Listing
April 2019
4 Reads

Vitrification of murine mature metaphase II oocytes perturbs DNA methylation reprogramming during preimplantation embryo development.

Cryobiology 2019 Apr 29;87:91-98. Epub 2019 Jan 29.

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China. Electronic address:

Accurate reprogramming of DNA methylation occurring in preimplantation embryos is critical for normal development of both fetus and placenta. Environmental stresses imposed on oocytes usually cause the abnormal DNA methylation reprogramming of early embryos. However, whether oocyte vitrification alters the reprogramming of DNA methylation (5 mC) and its derivatives in mouse preimplantation embryo development remains largely unknown. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.012DOI Listing
April 2019
2 Reads
1.587 Impact Factor

CT-guided percutaneous cryoablation combined with systemic chemotherapy for liver metastases from esophageal carcinoma: Initial experience.

Cryobiology 2019 Apr 28;87:99-104. Epub 2019 Jan 28.

Department of Interventional Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Huan Hu West Road, Tianjin, 300060, PR China; Key Laboratory of Cancer Prevention and Therapy, Tian Jin, Huan Hu West Road, Tianjin, 300060, PR China; Tianjin's Clinical Research Center for Cancer, Huan Hu West Road, Tianjin, 300060, PR China. Electronic address:

Objective: To explore the feasibility, safety and effectiveness of percutaneous cryoablation combined with systemic chemotherapy in the treatment of liver metastases from esophageal carcinoma (ECLM).

Materials And Methods: We retrospectively collected data of 16 patients who received CT-guided percutaneous cryoablation concurrent systemic chemotherapy for liver metastases after primary esophageal carcinoma resection. Functional Assessment of Cancer Therapy-General (FACT-G) was used for the assessment of quality of life (QOL), and overall survival (OS), progression-free survival (PFS) and complications were also evaluated. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.011DOI Listing
April 2019
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A preliminary study on the use of jenny colostrum to improve quality in extenders for freezing donkey semen.

Cryobiology 2019 Apr 24;87:110-114. Epub 2019 Jan 24.

Department of Animal Pathology, Faculty of Veterinary Medicine, Instituto Agroalimentario de Aragón IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain. Electronic address:

Sperm quality in donkeys (Equus asinus) after freezing thawing is still considered lower than that from other animals, including horses. The aim of this study was to test a new freezing extender supplemented with jenny colostrum on donkey sperm. After thawing, we evaluated sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional integrity by HOS-test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.009DOI Listing
April 2019
2 Reads

Production of mouse fetuses using spermatozoa exposed temporarily to high temperature or continuously to room temperature after freeze-drying in Na-free/K-rich EGTA buffer.

Cryobiology 2019 Apr 22;87:105-109. Epub 2019 Jan 22.

Department of Biological Sciences, Asahikawa Medical University, 2-1-1-1 Midorigaoka-higashi, Asahikawa, 078-8510, Japan. Electronic address:

Present study aimed to determine to what extent freeze-dried spermatozoa were able to withstand high-temperature conditions: transient increase in storage temperature and long-term exposure to room temperature. Mouse spermatozoa were freeze-dried in EGTA/Tris-HCl buffered solution alkalinized using KOH (K-ETBS, pH 7.7), and then stored for up to 7 months at 4 °C or 25 °C. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.010DOI Listing
April 2019
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Cryopreservation of mammalian embryos: Derivation of a method.

Cryobiology 2019 Feb 21;86:1-9. Epub 2019 Jan 21.

Department of Biological Sciences, University of New Orleans, New Orleans, LA, 70148, USA. Electronic address:

In 1972, a procedure was derived to cryopreserve mouse embryos. Over the past four decades, this procedure has been adapted to freeze embryos of more than twenty-five mammalian species. Cryopreservation of embryos has become a routine procedure in both veterinary and human medicine, having been used to freeze millions of embryos of mice and cattle, and many hundreds of thousands of human embryos. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.007DOI Listing
February 2019

Cryopreservation of shoot tips of recalcitrant and tropical species: Advances and strategies.

Cryobiology 2019 Apr 22;87:1-14. Epub 2019 Jan 22.

Retired, US Dept. of Agriculture and Department of Horticulture, Oregon State University, Corvallis, OR, 97331, USA. Electronic address:

There is a pressing need for practical and successful conservation efforts to establish long-term germplasm collections of recalcitrant and tropical species, given the challenge and threat that these plants are facing. Cryopreservation is the only way of conserving some of these species, especially those with temperature or desiccation sensitive (recalcitrant) seeds. This review covers reports on cryopreservation studies of shoot tips (apical and axillary) of tropical and subtropical plants. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.008DOI Listing

Metabolic reorganization in winter: Regulation of pyruvate dehydrogenase (PDH) during long-term freezing and anoxia.

Cryobiology 2019 Feb 10;86:10-18. Epub 2019 Jan 10.

Department of Biology, Carleton University, Ottawa, Ontario, Canada. Electronic address:

Wood frogs, Rana sylvatica, can undergo prolonged periods of whole body freezing during winter, locking as much as 65-70% of total body water into extracellular ice and imposing both anoxia and dehydration on their cells. Metabolic rate depression (MRD) is an adaptation used by R. sylvatica to survive these environmental stresses, where a finite amount of ATP generated through anaerobic metabolism is directed towards maintaining pro-survival functions, while most ATP-expensive cellular processes are temporarily reduced in function. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.006DOI Listing
February 2019
1 Read
1.587 Impact Factor

Isochoric conditions enable high subfreezing temperature pancreatic islet preservation without osmotic cryoprotective agents.

Cryobiology 2019 Feb 7;86:130-133. Epub 2019 Jan 7.

Department of Mechanical Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA. Electronic address:

While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day preservation at high subfreezing temperatures in an isochoric chamber without osmotic cryoprotective agents (CPA), highlights the potential of isochoric cryopreservation in an application of clinical value. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.003DOI Listing
February 2019
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Recovery and functionality of cryopreserved peripheral blood mononuclear cells using five different xeno-free cryoprotective solutions.

Cryobiology 2019 Feb 7;86:25-32. Epub 2019 Jan 7.

National-Local Associated Engineering Laboratory (Shenzhen) for Personalized Cellular Therapy, Nanshan, Shenzhen, Guangdong, China; Shenzhen Kenuo Medical Lab, Nanshan, Shenzhen, Guangdong, China. Electronic address:

In this study, we compared three commercially available and two widely used CPAs for their ability of cryopreserving PBMCs. Similar survival (81.0%) and recovery rate (73. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.004DOI Listing
February 2019
12 Reads
1.587 Impact Factor

Evaluation of changes in selected skin parameters under the influence of extremely low temperature.

Cryobiology 2019 Feb 7;86:19-24. Epub 2019 Jan 7.

Faculty of Physiotherapy, University School of Physical Education, Wroclaw, Poland.

The aim of the work was to evaluate changes in selected skin parameters under the influence of low temperature. The tests were conducted on a group of 20 women using whole-body cryotherapy. The average age of participants was 58. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.005DOI Listing
February 2019
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Effect of different olive oil-derived antioxidants (hydroxytyrosol and 3,4-dihydroxyphenylglycol) on the quality of frozen-thawed ram sperm.

Cryobiology 2019 Feb 3;86:33-39. Epub 2019 Jan 3.

Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Cordoba, Cordoba, 14014, Spain. Electronic address:

The aim of the present study was to evaluate the effect of the addition of different concentrations of two olive oil-derived antioxidants, hydroxytyrosol (3,4-dihydroxyphenylethanol, HT) and 3,4-dihydroxyphenylglycol (DHPG), on ovine semen during the freezing-thawing process. Sperm was collected, pooled and diluted with commercial extenders and then divided into aliquots supplemented with different concentrations (10 μg/ml, 30 μg/ml, 50 μg/ml and 70 μg/ml) of HT, DHPG and a mixture (MIX) of both antioxidants. A control group, without antioxidant, was also prepared. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.002DOI Listing
February 2019
8 Reads

Toxicity tests of cryoprotecting agents for Mytilus galloprovincialis (Lamark, 1819) early developmental stages.

Cryobiology 2019 Feb 3;86:40-46. Epub 2019 Jan 3.

Marine Biological Resources Functional Preservation Service, Estación de Ciencias Mariñas de Toralla, Universidade de Vigo, Illa de Toralla, 36331, Coruxo, Vigo, Spain. Electronic address:

Global aquaculture production of blue mussel has increased over last years. This work reaffirms the great potential of cryopreservation technique on mussel industry and overcome economic barriers a cause of a traditional and rudimentary management and continue growing. The aim of this work is to set some preliminary basis attending to toxicity of cryoprotecting agents (CPAs) on different development stages of Mytilus galloprovincialis as a start point to develop a stable cryopreservation protocol. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2019.01.001DOI Listing
February 2019
2 Reads

Effects of four disaccharides on nucleation and growth of ice crystals in concentrated glycerol aqueous solution.

Cryobiology 2019 Feb 28;86:47-51. Epub 2018 Dec 28.

Department of Refrigeration and Cryogenics Engineering, Hefei University of Technology, Hefei, 230009, Anhui, China.

Devitrification has been determined to be one of the major causes of cell death in cryopreservation by vitrification method. Reliable quantification of the nucleation and growth of ice crystals of devitrification is of great importance for the optimization of the vitrification solutions. In the present study, cryomicroscopy was used to investigate the nucleation and growth of ice crystals in concentrated glycerol aqueous solution (60 wt%) in the presence of sucrose, trehalose, maltose and lactose. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.12.006DOI Listing
February 2019
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Effects of different concentration and combinations of cryoprotectants on sperm quality, functional integrity in three Indian horse breeds.

Cryobiology 2019 Feb 18;86:52-57. Epub 2018 Dec 18.

ICAR-National Research Centre on Equines, Hisar, Haryana, 225001, India.

Traditionally Glycerol (Gly) is being used as major cryoprotectant and its toxicity could be a reason for the variation on stallion sperm freezability and fertility. In an effort to minimize Gly toxicity alternative cryoprotective agents like Di-methyl Formamide (DMF) have been investigated. The effect of the cryoprotectant and dose of cryoprotective agent varies from breed to breed and also from stallion to stallion within the same breed. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.12.005DOI Listing
February 2019
6 Reads
1.587 Impact Factor

The ideal holding time for boar semen is 24 h at 17 °C prior to short-cryopreservation protocols.

Cryobiology 2019 Feb 14;86:58-64. Epub 2018 Dec 14.

Swine research center, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil. Electronic address:

Boar semen cannot be immediately cryopreserved, it need be hold at 17 °C prior to cryopreservation, holding time has been used to improve cryopreserved boar semen, since holding time allows a prolonged interaction between spermatozoa and seminal plasma components. However, until now only few periods of holding time have been studied, and boar semen had been held at 17 °C for 24 h to facilitate its manufacture. Thus, this experiment aims to study the effect several holding time (0, 4, 8, 12, 24, 28 and 32 h) on boar spermatozoa post-thawed (PT) characteristics. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.12.004DOI Listing
February 2019
19 Reads

Micro-quantity straw as a carrier for cryopreservation of oligozoospermic semen samples: Effects of storage times and cryoprotectant.

Cryobiology 2019 Feb 8;86:65-70. Epub 2018 Dec 8.

Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Electronic address:

Application of an appropriate freezing carrier is crucial for improving post-thaw recovery of oligozoospermic samples. In this study, our purpose is developing a user-friendly, easy handling and close micro-quantity (MQ) straw along with different freezing media, for cryopreservation of oligozoospermic samples. Twenty oligozoospermic semen samples were collected and mixed with glycerol egg yolk citrate (GEYC) or Spermfreeze (SPF) medium. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.12.003DOI Listing
February 2019
8 Reads

Cryoprotectant toxicity in Caenorhabditis elegans.

Cryobiology 2019 Feb 4;86:71-76. Epub 2018 Dec 4.

Institute for Behavioral Genetics, University of Colorado, Boulder, USA; Department of Integrative Physiology, University of Colorado, Boulder, USA. Electronic address:

We have looked at the effects of the cryoprotectant M22 upon viability in the model organism C. elegans. M22 is a well-known vitrification solution which has been successfully used in the laboratory to preserve organs destined for transplantation. Read More

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February 2019
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Single layer colloid centrifugation technique improves motility, viability and chromatin integrity of ram spermatozoa after thawing.

Cryobiology 2019 Feb 4;86:77-83. Epub 2018 Dec 4.

Clinic for Reproduction and Large Animals, University of Ljubljana, Vet Faculty, Ljubljana, Slovenia.

The cell membrane of ram spermatozoa is more sensitive to the freezing process than in other species due to its composition. As a result, the quality and viability of frozen thawed ram spermatozoa are often poor, which together with the specific structure of the ewe's cervix are the main reasons for lower fertility in ewes after intracervical insemination. In the present study we investigated the effects of semen centrifugation through a single layer of a species-specific colloid (Androcoll-O) on post-thaw quality of ram spermatozoa. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.12.001DOI Listing
February 2019
4 Reads

Cryopreservation of embryos and larvae of the edible sea urchin loxechinus albus (Molina, 1782).

Cryobiology 2019 Feb 23;86:84-88. Epub 2018 Nov 23.

Cryopreservation Laboratory, Department of Marine Biology, Universidad Católica del Norte, Coquimbo, Chile.

The natural population of the edible red sea urchin, Loxechinus albus, is decreasing due to overfishing. The embryos and larvae of the species are highly useful for monitoring marine pollution, which makes it necessary to conserve gametes, embryos and larvae to facilitate their use in diverse areas of aquaculture and environmental quality monitoring. This need can be met by cryopreserving individuals representing the different developmental stages to provide an ongoing supply of genetic material of the species. Read More

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February 2019
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Production of F0 mice from embryonic stem cells injected eight-cell stage embryos which stored at refrigeration temperature.

Cryobiology 2019 Feb 22;86:89-94. Epub 2018 Nov 22.

Chongqing Academy of Animal Sciences, Chongqing, 402460, China; Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing, 402460, China; Key Laboratory of Pig Industry Sciences, Chongqing, 402460, China. Electronic address:

At refrigeration temperature, mouse embryos can retain their developmental ability for a couple of days. Previous research reports have focused on the effect of cool temperature on the development of 2-cell stage embryos, morulae or blastocysts and determined that the embryo still has the ability to produce offspring after about 48 h storage at refrigeration temperature. Here we examined whether refrigeration temperature affects the development of the eight-cell stage and if the stored eight-cell stage embryo can still be used as a host embryo for ES cell injection. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.11.004DOI Listing
February 2019
3 Reads
1.587 Impact Factor

Genetic suppression of cryoprotectant toxicity.

Cryobiology 2019 Feb 17;86:95-102. Epub 2018 Nov 17.

Institute for Behavioral Genetics, University of Colorado Boulder, USA; Department of Integrative Physiology, University of Colorado Boulder, USA. Electronic address:

We report here a new, unbiased forward genetic method that uses transposon-mediated mutagenesis to enable the identification of mutations that confer cryoprotectant toxicity resistance (CTR). Our method is to select for resistance to the toxic effects of M22, a much-studied whole-organ vitrification solution. We report finding and characterizing six mutants that are resistant to M22. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.11.003DOI Listing
February 2019
15 Reads

Apatite nanoparticles mediate intracellular delivery of trehalose and increase survival of cryopreserved cells.

Cryobiology 2019 Feb 17;86:103-110. Epub 2018 Nov 17.

Department of Cardiovascular Surgery, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China. Electronic address:

Cryopreservation of tissue cells is an important method to maintain cell viability and cellular function. However, cell viability and function are less than ideal by conventional cell cryopreservation methods, which may result in apoptosis and necrosis of cells in cryopreservation. Trehalose plays a role in maintaining cell structure and protecting cells from stress. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183027
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http://dx.doi.org/10.1016/j.cryobiol.2018.11.002DOI Listing
February 2019
18 Reads

Psychrotolerant bacteria isolated from the leaf apoplast of cold-adapted wild plants improve the cold resistance of bean (Phaseolus vulgaris L.) under low temperature.

Cryobiology 2019 Feb 10;86:111-119. Epub 2018 Nov 10.

Department of Biology Faculty of Science, Atatürk University, 25240 Erzurum, Turkey. Electronic address:

We have isolated psychrotolerant bacteria from the leaf apoplast of cold-adapted wild plants and aimed to investigate their effect on the cold resistance of bean (Phaseolus vulgaris L.). Based on the findings of 16S rRNA gene sequence analysis, 20 isolates belonging to 5 bacteria species (Pseudomonas fragi, P. Read More

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http://dx.doi.org/10.1016/j.cryobiol.2018.11.001DOI Listing
February 2019
2 Reads

Application of cryoablation to treat peritoneal carcinomatosis from gastric cancer in a rabbit model.

Cryobiology 2018 12 27;85:12-16. Epub 2018 Oct 27.

Department of General Surgery, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China. Electronic address:

Objectives: Peritoneal carcinomatosis is one of the causes of death in patients with advanced gastric cancer. We assumed that cryoablation could be applied as adjuvant therapy to control peritoneal carcinomatosis from gastric cancer.

Methods: We investigated the feasibility of cryoablation technique in rabbit model using a novel cryoablation balloon probe. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00112240183011
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http://dx.doi.org/10.1016/j.cryobiol.2018.10.263DOI Listing
December 2018
8 Reads