Search our Database of Scientific Publications and Authors

I’m looking for a

    25249 results match your criteria Coxsackieviruses

    1 OF 505

    Respiratory viruses among children with non-severe community-acquired pneumonia: A prospective cohort study.
    J Clin Virol 2018 Jun 6;105:77-83. Epub 2018 Jun 6.
    Department of Pediatrics, Federal University of Bahia School of Medicine, Salvador, Brazil. Electronic address:
    Background: Community-acquired pneumonia (CAP) causes a major burden to the health care system among children under-5 years worldwide. Information on respiratory viruses in non-severe CAP cases is scarce.

    Objectives: To estimate the frequency of respiratory viruses among non-severe CAP cases. Read More

    An Environmental Surveillance in Uruguay Reveals the Presence of Highly Divergent Types of Human Enterovirus Species C and a High Frequency of Species A and B Types.
    Food Environ Virol 2018 Jun 16. Epub 2018 Jun 16.
    Laboratorio de Virología Molecular, CENUR Litoral Norte, Sede Salto, Universidad de la República, Gral. Rivera 1350, 50000, Salto, Uruguay.
    Information about Human Enterovirus circulation in Uruguay is scarce. The aim of this study was to generate the first description about their circulation in the country through the study of sewage samples collected before and after the switch from Oral Poliovirus Vaccine to Inactivated Poliovirus Vaccine. Viruses were concentrated by an adsorption-elution to a negatively charged membrane, and real-time quantitative PCR and qualitative PCR methods were used to detect, quantify, and characterize enteroviruses. Read More

    Analytical validation of viral CNS Flow Chip kit for detection of acute meningitis and encephalitis.
    J Virol Methods 2018 Jun 11. Epub 2018 Jun 11.
    Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Instituto de Investigación Biosanitaria de Granada, Avda. Fuerzas Armadas, 2, Granada, 18014, Spain.
    A new molecular assay (Viral CNS Flow Chip kit, Master Diagnóstica, Spain) has been developed for the detection of eight viruses causing acute meningitis and encephalitis, i.e. herpes simplex viruses 1-2, varicella zoster virus, human enterovirus, human parechovirus, Toscana virus, human cytomegalovirus and Epstein Barr virus. Read More

    Virus-like particles and enterovirus antigen found in the brainstem neurons of Parkinson's disease.
    F1000Res 2018 9;7:302. Epub 2018 Mar 9.
    Virus Reference Department, National Infection Service, Public Health England, London, NW9 5EQ, UK.
    In a previous study on encephalitis lethargica, we identified an enterovirus in autopsy brain material. Transmission electron microscopy (TEM), immunohistochemistry (IHC) and molecular analysis were employed.  Our present objective was to investigate, using a similar approach, as to whether virus-like particles (VLP) and enterovirus antigen are present in Parkinson's disease (PD) brainstem neurons. Read More

    Coxsackievirus A24 Variant Associated with Acute Haemorrhagic Conjunctivitis Cases, French Guiana, 2017.
    Intervirology 2018 Jun 13:1-5. Epub 2018 Jun 13.
    Institut Pasteur, Unité de Biologie des Virus Entériques, INSERM U994, Paris, France.
    In 2017, numerous cases of acute haemorrhagic conjunctivitis (AHC) were reported in the Caribbean and in South America. Preliminary reports identified adenoviruses and enteroviruses in some patient samples but, until now, none of the etiologic agents have been fully characterized. We report the full-length genomic sequences of 4 coxsackievirus A24 (CV-A24) isolates collected from AHC patients in French Guiana during this outbreak (May and June 2017). Read More

    Changes in the EV-A71 Genome through Recombination and Spontaneous Mutations: Impact on Virulence.
    Viruses 2018 06 12;10(6). Epub 2018 Jun 12.
    Centre for Virus and Vaccine Research, School of Science and Technology, Sunway University, Kuala Lumpur, Selangor 47500, Malaysia.
    Enterovirus 71 (EV-A71) is a major etiological agent of hand, foot and mouth disease (HFMD) that mainly affects young children less than five years old. The onset of severe HFMD is due to neurological complications bringing about acute flaccid paralysis and pulmonary oedema. In this review, we address how genetic events such as recombination and spontaneous mutations could change the genomic organization of EV-A71, leading to an impact on viral virulence. Read More

    Evolutionary histories of coxsackievirus B5 and swine vesicular disease virus reconstructed by phylodynamic and sequence variation analyses.
    Sci Rep 2018 Jun 11;8(1):8821. Epub 2018 Jun 11.
    Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung, 807, Taiwan.
    Coxsackievirus (CV)-B5 is a common human enterovirus reported worldwide; swine vesicular disease virus (SVDV) is a porcine variant of CV-B5. To clarify the transmission dynamics and molecular basis of host switching between CV-B5 and SVDV, we analysed and compared the VP1 and partial 3D gene regions of these two viruses. Spatiotemporal dynamics of viral transmission were estimated using a Bayesian statistical inference framework. Read More

    Fibroblast-stimulating lipopeptide-1 as a potential mucosal adjuvant enhances mucosal and systemic immune responses to enterovirus 71 vaccine.
    Vaccine 2018 Jun 8. Epub 2018 Jun 8.
    Department of Medical Research, National Taiwan University Hospital, Taipei, Taiwan; Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan. Electronic address:
    To prevent viral infection at the site of entry, mucosal vaccines are potent tools for inducing IgA secretion for defense. Because Toll-like receptor (TLR) ligands serve as strong adjuvants, two ligands that mimic the structure of mycoplasmal and bacterial lipopeptides represent interesting vaccine candidates. Pam3CSK4, a synthetic triacylated lipopeptide, interacts with TLR2/1. Read More

    Pathogen Clearance and New Respiratory Tract Infections Among Febrile Children in Zanzibar Investigated With Multitargeting Real-Time Polymerase Chain Reaction on Paired Nasopharyngeal Swab Samples.
    Pediatr Infect Dis J 2018 Jul;37(7):643-648
    From the Department of Infectious Diseases.
    Background: New molecular methods have revealed frequent and often polymicrobial respiratory infections in children in low-income settings. It is not known whether presence of multiple pathogens is due to prolonged infections or to frequent exposure. The aim of this study was to analyze short-term pathogen clearance from nasopharynx and the rate of new respiratory tract infections in febrile preschool children. Read More

    Genome-wide linear B-cell epitopes of enterovirus 71 in a hand, foot and mouth disease (HFMD) population.
    J Clin Virol 2018 Jun 3;105:41-48. Epub 2018 Jun 3.
    Department of pathogen diagnosis and biosafety, Shanghai public health clinical center, Fudan University, Shanghai, 201508, PR China. Electronic address:
    Background: Enteroviruses cause hand, foot and mouth disease (HFMD). The host B-cells recognize the viral proteins and provoke humoral responses. Deciphering the B-cell responses to the viral epitopes helps diagnosis and vaccine development. Read More

    Metagenomic analysis of Sichuan takin fecal sample viromes reveals novel enterovirus and astrovirus.
    Virology 2018 Jun 7;521:77-91. Epub 2018 Jun 7.
    State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China; Department of Microbiology, The University of Hong Kong, Hong Kong, China; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong, China. Electronic address:
    The Sichuan takin inhabits the bamboo forests in the Eastern Himalayas and is considered as a national treasure of China with the highest legal protection and conservation status considered as vulnerable according to The IUCN Red List of Threatened Species. In this study, fecal samples of 71 Sichuan takins were pooled and deep sequenced. Among the 103,553 viral sequences, 21,961 were assigned to mammalian viruses. Read More

    Pyroptosis induced by enterovirus A71 infection in cultured human neuroblastoma cells.
    Virology 2018 Jun 7;521:69-76. Epub 2018 Jun 7.
    Institute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention, 172 JiangSu Road, Nanjing 210009, China; Key Laboratory of Infectious Diseases, Nanjing Medical University, Nanjing 211166, China. Electronic address:
    Enterovirus A71 (EV-A71) infection can cause hand, foot and mouth disease (HFMD), and even fatal meningoencephalitis. Unfortunately, there is currently no effective treatment for EV-A71 infection due to the lack of understanding of the mechanism of neurological diseases. In this study, we employed SH-SY5Y human neuroblastoma cells to explore the roles of caspase-1 in neuropathogenesis. Read More

    3D-quantitative structure-activity relationship study for the design of novel enterovirus A71 3C protease inhibitors.
    Chem Biol Drug Des 2018 Jun 7. Epub 2018 Jun 7.
    State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300353, P.R. China.
    A three-dimensional quantitative structure-activity relationships model of enterovirus A71 3C protease inhibitors was constructed in this study. The protein-ligand interaction fingerprint was analyzed to generate a pharmacophore model. A predictive and reliable three-dimensional quantitative structure-activity relationships model was built based on the Flexible Alignment of AutoGPA. Read More

    A cross-sectional seroepidemiology study of EV-D68 in China.
    Emerg Microbes Infect 2018 Jun 6;7(1):99. Epub 2018 Jun 6.
    National Institute for Food and Drug Control, Beijing, China.
    Enterovirus 68 (EV-D68) is associated with respiratory diseases, such as acute upper respiratory tract infections (URTIs), lower respiratory tract infections (LRTIs), pneumonia, neurological diseases, and acute flaccid myelitis (AFM). In recent years, there have been global outbreaks of EV-D68 epidemics. However, there is no effective vaccine against EV-D68, and the understanding of the seroprevalence characteristics of EV-D68 is limited. Read More

    Author Correction: Mutations in VP1 and 5'-UTR affect enterovirus 71 virulence.
    Sci Rep 2018 Jun 4;8(1):8744. Epub 2018 Jun 4.
    National Institute of Infectious Disease and Vaccinology, National Health Research Institutes, Zhunan, 350, Taiwan.
    A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. Read More

    Efficient Humoral and Cellular Immune Responses Induced by a Chimeric Virus-like Particle Displaying the Epitope of EV71 without Adjuvant.
    Biomed Environ Sci 2018 May;31(5):343-350
    National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
    Objective: To eliminate the side effects of aluminum adjuvant and His-tag, we constructed chimeric VLPs displaying the epitope of EV71 (SP70) without His-tagged. Then evaluating whether the VLPs could efficiently evoke not only humoral but also cellular immune responses against EV71 without adjuvant.

    Methods: The fusion protein was constructed by inserting SP70 into the MIR of truncated HBcAg sequence, expressed in E. Read More

    [Spatial-temporal analysis of enterovirus infection in Macao Special Administrative Region, China, 2011-2016].
    Zhonghua Liu Xing Bing Xue Za Zhi 2018 May;39(5):661-663
    Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou 510515, China.
    To understand the spatial-temporal distribution of enterovirus infection in Macao Special Administrative Region, China, from 2011 to 2016. The incidence data of enterovirus infections in child care settings and primary schools in Macao during this period, which were confirmed by the Health Bureau, were used for the spatial-temporal analysis. Bernoulli model was used as probability model. Read More

    Age-related prevalence of common upper respiratory pathogens, based on the application of the FilmArray Respiratory panel in a tertiary hospital in Greece.
    Medicine (Baltimore) 2018 Jun;97(22):e10903
    The FilmArray Respiratory Panel (FA-RP) is an FDA certified multiplex PCR that can detect 17 viruses and 3 bacteria responsible for upper respiratory tract infections, thus it is potentially useful to the assessment of the age-related prevalence of these pathogens.In this observational study, we retrospectively analyzed the results of all the respiratory samples, which had been processed during 1 year-period (November 2015 to November 2016) with the FA-RP, in the Central Laboratories of Hygeia & Mitera General Hospitals of Athens, Greece. In order to have an age-related distribution, the following age groups were implemented: (<2), (≥2, <5), (≥5, <10), (≥10, <18), (≥18, <45), (≥45, <65), and (≥65) years old. Read More

    Cytomegalovirus myocarditis in a patient with systemic lupus erythematosus (SLE) successfully treated with ganciclovir.
    IDCases 2018 26;12:4-6. Epub 2018 Feb 26.
    Infectious Diseases Clinic, University Hospital "S. Maria", University of Perugia, Terni, Italy.
    Myocarditis is an inflammatory disease of the myocardium. Viruses, such as enterovirus, adenovirus, parvovirus B19, HHV6 or cytomegalovirus (CMV) and autoimmune diseases are recognized causes of myocarditis. We describe the clinical case of a young Indian woman with SLE and a concomitant acute CMV related myocarditis with favourable outcome after ganciclovir therapy. Read More

    Amino acid variation at VP1-145 of enterovirus 71 determines attachment receptor usage and neurovirulence in human scavenger receptor B2 transgenic mice.
    J Virol 2018 May 30. Epub 2018 May 30.
    Neurovirology Project, Tokyo Metropolitan Institute of Medical Science, 2-1-6, Kamikitazawa, Setagaya-ku, Tokyo, Japan
    Infection of enterovirus 71 (EV71) is affected by cell surface receptors, including the scavenger receptor B2, which are required for viral uncoating, and attachment receptors, such are heparan sulfate (HS), which bind virus, but do not support uncoating. Amino acid residue 145 of the capsid protein VP1 affects viral binding to HS, and virulence in mice. However, the contribution of this amino acid to pathogenicity in humans is not known. Read More

    VP1 amino acid residue 145 of enterovirus 71 is a key residue for its receptor attachment and resistance to neutralizing antibody during cynomolgus monkey infection.
    J Virol 2018 May 30. Epub 2018 May 30.
    Neurovirology Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    Enterovirus 71 (EV71) is a causative agent of hand, foot, and mouth disease and sometimes causes severe or fatal neurological complications. The amino acid at VP1-145 determines virological characteristics of EV71. Viruses with glutamic acid (E) at VP1-145 (VP1-145E) are virulent in neonatal mice and transgenic mice expressing human scavenger receptor B2, whereas those with glutamine (Q) or glycine (G) are not. Read More

    Infections: a double-edge sword in autoimmunity.
    Curr Opin Rheumatol 2018 Jul;30(4):365-372
    Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, affiliated to the Sackler Faculty of Medicine Tel-Aviv University.
    Purpose Of Review: Infections play a role in the pathogenesis of autoimmune diseases (AID). Several bacterial and viral pathogens play a double role, as both inducers and inhibitors of AID. In this review, we will present current evidence and discuss different aspects of this notion. Read More

    Hand, Foot and Mouth Disease in the Andaman Islands, India.
    Indian Pediatr 2018 May;55(5):408-410
    Regional Medical Research Centre (ICMR), Port Blair, A and N Islands, India. Correspondence to: Dr Vijayachari Paluru, Director and Scientist-G, Regional Medical Research Centre (ICMR), Post bag No. 13, Dollygunj, Port Blair - 744 101, Andaman and Nicobar Islands, India.
    Objective: To investigate an outbreak of Hand, foot and mouth disease (HFMD) in Andaman Islands during 2013.

    Methods: Epidemiological, clinical data and samples were collected from HFMD patients who attended selected hospitals. Data were analyzed and samples were processed for detection of Enterovirus and further confirmed by sequencing. Read More

    Reinfection hazard of hand-foot-mouth disease in Wuhan, China, using Cox-proportional hazard model.
    Epidemiol Infect 2018 May 30:1-6. Epub 2018 May 30.
    Wuhan Center for Disease Prevention and Control,Wuhan 430015,China.
    Hand-foot-mouth disease (HFMD) is an acute infectious disease caused by serotypes of the enterovirus (EV) family. HFMD reinfection occurs commonly in lack of cross-protection between different EV serotypes. In this study, we investigated the hazards of HFMD reinfection using Cox-proportional hazard model. Read More

    High frequency of Polio-like Enterovirus C strains with differential clustering of CVA-13 and EV-C99 subgenotypes in a cohort of Malawian children.
    Arch Virol 2018 May 28. Epub 2018 May 28.
    Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands.
    Enteroviruses (EVs) are among the most commonly detected viruses infecting humans worldwide. Although the prevalence of EVs is widely studied, the status of EV prevalence in sub-Saharan Africa remains largely unknown. The objective of our present study was therefore to increase our knowledge on EV circulation in sub-Saharan Africa. Read More

    Structure-activity relationship study of itraconazole, a broad-range inhibitor of picornavirus replication that targets oxysterol-binding protein (OSBP).
    Antiviral Res 2018 May 26;156:55-63. Epub 2018 May 26.
    Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584CL Utrecht, the Netherlands. Electronic address:
    Itraconazole (ITZ) is a well-known, FDA-approved antifungal drug that is also in clinical trials for its anticancer activity. ITZ exerts its anticancer activity through several disparate targets and pathways. ITZ inhibits angiogenesis by hampering the functioning of the vascular endothelial growth receptor 2 (VEGFR2) and by indirectly inhibiting mTOR signaling. Read More

    Metagenomic analysis of the RNA fraction of the fecal virome indicates high diversity in pigs infected by porcine endemic diarrhea virus in the United States.
    Virol J 2018 05 25;15(1):95. Epub 2018 May 25.
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1907 ISU C-Drive, VMRI#1, Ames, IA, 50011, USA.
    Background: Emergence and re-emergence of porcine epidemic diarrhea virus (PEDV) in North America, Asia and Europe has caused severe economic loss to the global swine industry. However, the virome of PEDV infected pigs and its effect on disease severity remains unknown. The advancements of sequencing technology have made it possible to characterize the entire microbiome of different body sites for any host. Read More

    Hopkins syndrome following the first episode of bronchial asthma associated with enterovirus D68: a case report.
    BMC Neurol 2018 May 23;18(1):71. Epub 2018 May 23.
    Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
    Background: Hopkins syndrome (HS) is a rare disorder presenting with acute flaccid paralysis of the limbs following an asthma attack. Neurologists encounter a diagnostic challenge if patients without a history of bronchial asthma develop neurologic features mimicking HS following acute respiratory distress. We report a case of HS occurring after a first episode of bronchial asthma associated with enterovirus D68 infection. Read More

    Nervous System Infections and the Global Traveler.
    Semin Neurol 2018 Apr 23;38(2):247-262. Epub 2018 May 23.
    Division of Critical Care and Hospitalist Neurology, Department of Neurology, Columbia University Medical Center, New York, New York.
    Neurological complications of infectious diseases are associated with high rates of morbidity and mortality. It is imperative that neurologists be up-to-date on current developments including typical and atypical presentations of neurological infections in travelers, diagnostic and treatment recommendations, and emerging pathogen resistance patterns to avoid fatal outcomes and long-term sequelae. This article will address concepts of emerging and reemerging infectious diseases, and will provide updates on the neurological manifestations of select emerging and reemerging infections, including Ebola virus, bacterial meningitis, enterovirus 71, Zika virus, cerebral malaria, and Japanese encephalitis. Read More

    Involvement of the renin-angiotensin system in the progression of severe hand-foot-and-mouth disease.
    PLoS One 2018 23;13(5):e0197861. Epub 2018 May 23.
    Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, Henan Province, China.
    Background: Hand-foot-and-mouth disease (HFMD) is generally considered as a mild exanthematous disease to infants and young children worldwide. HFMD cases are usually mild and self-limiting but for few cases leads to complicated severe clinical outcomes, and even death. Previous studies have indicated that serum Ang II levels in patients with H7N9 infection were related to the severity of infection. Read More

    Full-length and defective enterovirus G genomes with distinct torovirus protease insertions are highly prevalent on a Chinese pig farm.
    Arch Virol 2018 May 21. Epub 2018 May 21.
    School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China.
    Recombination occurs frequently between enteroviruses (EVs) which are classified within the same species of the Picornaviridae family. Here, using viral metagenomics, the genomes of two recombinant EV-Gs (strains EVG 01/NC_CHI/2014 and EVG 02/NC_CHI/2014) found in the feces of pigs from a swine farm in China are described. The two strains are characterized by distinct insertion of a papain-like protease gene from toroviruses classified within the Coronaviridae family. Read More

    Applicability of integrated cell culture reverse transcriptase quantitative PCR (ICC-RTqPCR) for the simultaneous detection of the four human enteric enterovirus species in disinfection studies.
    J Virol Methods 2018 08 17;258:35-40. Epub 2018 May 17.
    National Risk Management Research Laboratory, United States Environmental Protection Agency, Cincinnati, OH, USA.
    A newly developed integrated cell culture reverse transcriptase quantitative PCR (ICC-RTqPCR) method and its applicability in UV disinfection studies is described. This method utilizes a cell culture system coupled with four RTqPCR assays to detect four species of human enterovirus (e.g. Read More

    Outcomes following severe hand foot and mouth disease: A systematic review and meta-analysis.
    Eur J Paediatr Neurol 2018 Apr 20. Epub 2018 Apr 20.
    Oxford University Clinical Research Unit, Ha Noi, Viet Nam; Nuffield Department of Medicine, Centre for Tropical Medicine and Global Health, Oxford University, Oxford, UK.
    Background: Hand, foot and mouth disease (HFMD) caused by enterovirus A71 (EV-A71) is associated with acute neurological disease in children. This study aimed to estimate the burden of long-term sequelae and death following severe HFMD.

    Methods: This systematic review and meta-analysis pooled all reports from English and Chinese databases including MEDLINE and Wangfang on outbreaks of clinically diagnosed HFMD and/or laboratory-confirmed EV-A71 with at least 7 days' follow-up published between 1st January 1966 and 19th October 2015. Read More

    A reverse genetics system for enterovirus D68 using human RNA polymerase I.
    Virus Genes 2018 May 17. Epub 2018 May 17.
    School of Life Sciences, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, China.
    Human enterovirus D68 (EV-D68) is a highly contagious virus, which causes respiratory tract infections. However, no effective vaccines are currently available for controlling EV-D68 infection. Here, we developed a reverse genetics system to recover EV-D68 minireplicons and infectious EV-D68 from transfected plasmids using the RNA polymerase I (Pol I) promoter. Read More

    A virus-like particle-based tetravalent vaccine for hand, foot, and mouth disease elicits broad and balanced protective immunity.
    Emerg Microbes Infect 2018 May 18;7(1):94. Epub 2018 May 18.
    Vaccine Research Center, CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China.
    Hand, foot, and mouth disease (HFMD) is an infectious disease that mainly affects infants and children, causing considerable morbidity and mortality worldwide. HFMD is commonly caused by enterovirus 71 (EV71) and coxsackieviruses A16 (CVA16), A6 (CVA6), and A10 (CVA10). Formalin-inactivated EV71 vaccines are currently available in China; however, these vaccines fail to confer cross-protection against infections by other HFMD-causing enteroviruses, highlighting the necessity of developing a multivalent HFMD vaccine. Read More

    MicroRNA-302 Cluster Downregulates Enterovirus 71-Induced Innate Immune Response by Targeting KPNA2.
    J Immunol 2018 May 18. Epub 2018 May 18.
    State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430072, China;
    Enterovirus 71 (EV71) induces significantly elevated levels of cytokines and chemokines, leading to local or systemic inflammation and severe complications. As shown in our previous study, microRNA (miR) 302c regulates influenza A virus-induced IFN expression by targeting NF-κB-inducing kinase. However, little is known about the role of the miR-302 cluster in EV71-mediated proinflammatory responses. Read More

    Successful treatment of fulminant neonatal enteroviral myocarditis in monochorionic diamniotic twins with cardiopulmonary support, intravenous immunoglobulin and pocapavir.
    BMJ Case Rep 2018 May 18;2018. Epub 2018 May 18.
    Division of Pediatric Cardiology, St Louis Children's Hospital, Washington University School of Medicine, St Louis, Missouri, USA.
    Neonatal cardiogenic shock most commonly occurs due to critical congenital heart disease, sepsis, metabolic disorder or arrhythmias. In particular, enterovirus infections are common in the neonatal period, and patients can present with fulminant myocarditis. Early recognition is imperative due to its high morbidity and mortality without prompt and aggressive treatment. Read More

    Willingness and influential factors of parents to vaccinate their children with novel inactivated enterovirus 71 vaccines in Guangzhou, China.
    Vaccine 2018 06;36(26):3772-3778
    Guangzhou Centre for Diesease Control and Prevention, Guanghzou, China.
    Hand, foot and mouth disease (HFMD) primarily affects children younger than 5 years of age. Recently, HFMD has ranked as the top notifiable infectious disease in China. In December 2015, China approved two novel inactivated enterovirus 71 vaccines (EV71 vaccines) for HFMD. Read More

    Salivary anti-coxsackievirus-B4 neutralizing activity and pattern of immune parameters in patients with type 1 diabetes: a pilot study.
    Acta Diabetol 2018 May 17. Epub 2018 May 17.
    Université de Lille, Faculté de Médecine, CHU de Lille, Laboratoire de Virologie EA3610, 59000, Lille, France.
    Aims: Enteroviruses, especially coxsackieviruses B (CV-B), have been associated with the pathogenesis of type 1 diabetes (T1D). An anti-CV-B4 neutralizing activity in saliva of T1D patients was previously reported. Our aim was to study the association between the saliva anti-CV-B4 neutralizing activity and immune parameters in T1D patients in comparison with non-diabetic individuals. Read More

    Development and evaluation of a rapid recombinase polymerase amplification assay for the detection of human enterovirus 71.
    Arch Virol 2018 May 16. Epub 2018 May 16.
    Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, 510515, China.
    Enterovirus 71 (EV71) is one of the most common pathogens of hand, foot, and mouth disease (HFMD). A rapid reverse transcription recombinase polymerase amplification (RT-RPA) assay was established to detect EV71 subgenotype C4 (EV71-C4). The 95% detection limit of the RT-RPA was 3. Read More

    Mast cells contribute to Enterovirus 71 infection-induced pulmonary edema in neonatal mice.
    Lab Invest 2018 May 15. Epub 2018 May 15.
    Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, Henan, People's Republic of China.
    Enterovirus (EV) 71 infection has been widely acknowledged as the leading cause of severe hand, foot and mouth disease (HFMD), which may rapidly lead to fatal pulmonary edema. In this study, we established a mouse model for EV71 infection exhibiting high incidence of severe symptoms with pulmonary edema. Mast cells (MCs) accumulation, activation and allergic inflammation were found in the brains, lungs and skeletal muscle of mice after EV71 infection, especially in the lungs of mice. Read More

    Evaluation of MinION nanopore sequencing for rapid enterovirus genotyping.
    Virus Res 2018 07 12;252:8-12. Epub 2018 May 12.
    Genecology Research Centre, School of Science and Engineering, University of the Sunshine Coast, 90 Sippy Downs Dr, Sippy Downs, Queensland, Australia; Division of Experimental Therapeutics, Department of Medicine, Columbia University, NY, USA. Electronic address:
    Enteroviruses (EV) are associated with a range of serious infections, including aseptic meningitis, hand foot and mouth disease, myocarditis, acute flaccid paralysis and encephalitis. Improved methods for assessing EV genotypic diversity could assist molecular epidemiology studies, clinical diagnosis and environmental surveillance. We report new methods for EV genome amplification, and subsequent genotyping using the miniaturised MinION sequencing device. Read More

    Tracking enteric viruses in green vegetables from central Argentina: potential association with viral contamination of irrigation waters.
    Sci Total Environ 2018 May 11;637-638:665-671. Epub 2018 May 11.
    Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n - Ciudad Universitaria, CP 5000 Córdoba, Argentina.
    Consumption of green vegetable products is commonly viewed as a potential risk factor for infection with enteric viruses. The link between vegetable crops and fecally contaminated irrigation water establishes an environmental scenario that can result in a risk to human health. The aim of this work was to analyze the enteric viral quality in leafy green vegetables from Córdoba (Argentina) and its potential association with viral contamination of irrigation waters. Read More

    Rapid detection of hand, foot and mouth disease enterovirus genotypes by multiplex PCR.
    J Virol Methods 2018 08 26;258:7-12. Epub 2018 May 26.
    Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong, Taishan Medical College, Taian, 271000, Shandong, China. Electronic address:
    Hand, foot and mouth disease (HFMD) is a pediatric disease associated with infection by enterovirus (EV) genotypes. The major HFMD EV pathogens are enterovirus A71 (EVA71) and coxsackievirus A16 (CVA16); however, recently, coxsackievirus A6 (CVA6) and coxsackievirus A10 (CVA10) have also emerged. EV genotypes cannot be distinguished on clinical grounds and a new methodology for the rapid detection of the four major HFMD EV genotypes is urgently required. Read More

    Most commonly isolated viruses in asthma exacerbation and their correlation with eosinophil and total serum immunoglobulin E levels.
    Arch Argent Pediatr 2018 06;116(3):192-197
    Departamento de Pediatría, Hospital Civil de Guadalajara Dr. Juan I. Menchaca, Jalisco, México.
    Background: Asthma exacerbations are still a cause of hospitalization at the Emergency Department. The triggers of asthma exacerbations include allergens and infections -mostly viral-. The objective of this study was to establish the relationship between viruses detected during an asthma exacerbation and eosinophil and serum immunoglobulin E (IgE) levels in pediatric patients. Read More

    Microfluidic PCR Amplification and MiSeq Amplicon Sequencing Techniques for High-Throughput Detection and Genotyping of Human Pathogenic RNA Viruses in Human Feces, Sewage, and Oysters.
    Front Microbiol 2018 27;9:830. Epub 2018 Apr 27.
    Department of Civil and Environmental Engineering, Tohoku University, Sendai, Japan.
    Detection and genotyping of pathogenic RNA viruses in human and environmental samples are useful for monitoring the circulation and prevalence of these pathogens, whereas a conventional PCR assay followed by Sanger sequencing is time-consuming and laborious. The present study aimed to develop a high-throughput detection-and-genotyping tool for 11 human RNA viruses [Aichi virus; astrovirus; enterovirus; norovirus genogroup I (GI), GII, and GIV; hepatitis A virus; hepatitis E virus; rotavirus; sapovirus; and human parechovirus] using a microfluidic device and next-generation sequencer. Microfluidic nested PCR was carried out on a 48. Read More

    Caspase-3 Inhibition Attenuates the Cytopathic Effects of EV71 Infection.
    Front Microbiol 2018 26;9:817. Epub 2018 Apr 26.
    Institute of Virology and AIDS Research, The First Hospital of Jilin University, Jilin University, Changchun, China.
    Previous studies demonstrate that human enterovirus 71 (EV71), a primary causative agent for hand, foot, and mouth disease, activates caspase-3 through the non-structural viral 3C protein to induce host cell apoptosis; however, until now it was unclear how 3C activates caspase-3 and how caspase-3 activation affects viral production. Our results demonstrate that 3C binds caspase-8 and caspase-9 but does not directly bind caspase-3 to activate them, and that the proteolytic activity of 3C is required by the activation of caspase-8, caspase-9, and caspase-3. Inhibition of caspase-3 activity attenuates apoptosis in 3C-transfected cells. Read More

    Detection of nineteen enteric viruses in raw sewage in Japan.
    Infect Genet Evol 2018 May 16;63:17-23. Epub 2018 May 16.
    Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan; Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan. Electronic address:
    One-year surveillance for enteric viruses in raw sewage was conducted in Kansai area, central part of Japan from July 2015 to June 2016. The raw sewage was collected monthly from an inlet polluted pool and was concentrated by polyethylene glycol (PEG) precipitation. Twelve sewage samples were screened for nineteen kinds of enteric viruses by using RT-PCR method and further analyzed by nucleotide sequencing. Read More

    1 OF 505