25,958 results match your criteria Coxsackieviruses


Respiratory virus infection amongst hospitalized adult patients with or without clinically-apparent respiratory infection: a prospective cohort study.

Clin Microbiol Infect 2019 Apr 17. Epub 2019 Apr 17.

Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China; Department of Clinical Microbiology and Infection Control, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China; Department of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, China; State Key Laboratory for Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China. Electronic address:

Objectives: We aimed to determine the viral epidemiology and clinical characteristics of patients with and those without clinically-apparent respiratory tract infection.

Methods: This prospective cohort study was conducted during the 2018 winter influenza season. Adult patients with fever/respiratory symptoms (fever/RS group) were age- and sex-matched with patients without fever/respiratory symptoms (non-fever/RS group) in a 1:1 ratio. Read More

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http://dx.doi.org/10.1016/j.cmi.2019.04.012DOI Listing

A Common Receptor Found for Echoviruses.

Trends Microbiol 2019 Apr 16. Epub 2019 Apr 16.

Department of Biological and Environmental Science, Nanoscience Center, University of Jyväskylä, PO, Box 35, FI-40014 Jyväskylä, Finland. Electronic address:

It has remained a puzzle why infants, during the first weeks of life, are especially prone to enterovirus infections. New work (Proc. Natl. Read More

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http://dx.doi.org/10.1016/j.tim.2019.03.010DOI Listing

Genetic landscape and macro-evolution of co-circulating Coxsackieviruses A and Vaccine-derived Polioviruses in the Democratic Republic of Congo, 2008-2013.

PLoS Negl Trop Dis 2019 Apr 19;13(4):e0007335. Epub 2019 Apr 19.

Biology of Enteric Viruses Unit, Institut Pasteur, Paris, France.

Enteroviruses (EVs) are among the most common viruses infecting humans worldwide but only a few Non-Polio Enterovirus (NPEV) isolates have been characterized in the Democratic Republic of Congo (DR Congo). Moreover, circulating vaccine-derived polioviruses (PVs) [cVDPVs] isolated during multiple outbreaks in DR Congo from 2004 to 2018 have been characterized so far only by the sequences of their VP1 capsid coding gene. This study was carried to i) investigate the circulation and genetic diversity of NPEV and polio vaccine isolates recovered from healthy children and Acute Flaccid Paralysis (AFP) patients, ii) evaluate the occurrence of genetic recombination among EVs belonging to the Enterovirus C species (including PVs) and iii) identify the virological factors favoring multiple emergences of cVDPVs in DR Congo. Read More

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http://dx.doi.org/10.1371/journal.pntd.0007335DOI Listing

Complete Genome Sequence of a Human Enterovirus C99 Strain Isolated from a Healthy Child in Yunnan, China, in 2013.

Microbiol Resour Announc 2019 Apr 18;8(16). Epub 2019 Apr 18.

Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, People's Republic of China

The complete genome sequence of a human enterovirus C99 strain isolated from a healthy child in Yunnan, China, in 2013 was determined. The isolate belonged to genotype C, according to phylogenetic and homogeneity analyses. Read More

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http://dx.doi.org/10.1128/MRA.01489-18DOI Listing

Enterovirus A71 Containing Codon-Deoptimized VP1 and High-Fidelity Polymerase as Next Generation Vaccine Candidate.

J Virol 2019 Apr 17. Epub 2019 Apr 17.

Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan

Enterovirus A71 (EV-A71) is the major pathogen which causes hand-foot-and-mouth disease (HFMD) which occasionally results in severe neurological complications. In this study, we developed four EV-A71 (rgEV-A71) strains by reverse genetics procedures as possible vaccine candidates. The four rgEV-A71 viruses contained various codon deoptimized VP1 capsid protein (VP1-CD) showed similar replication rate and antigenicity to wild-type virus, while a fifth virus, rg4643C4VP-CD, was unable to form plaques, but was still able to examined by TCID50 titers which were similar to the others, thus indicating the effect of CD on plaque formation. Read More

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http://dx.doi.org/10.1128/JVI.02308-18DOI Listing
April 2019
1 Read

Association Between Children's Hospital Visitor Restrictions and Healthcare-Associated Viral Respiratory Infections: A Quasi-Experimental Study.

J Pediatric Infect Dis Soc 2019 Apr 16. Epub 2019 Apr 16.

Departments of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

We investigated the effect of annual winter visitor restrictions on hospital respiratory virus transmission. The healthcare-associated (HA) viral respiratory infection (VRI) transmission index (number of HA VRIs per 100 inpatient community-associated VRIs) was 59% lower during the months in which visitor restrictions were implemented. These data prompt consideration for instituting year-round visitor restrictions. Read More

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http://dx.doi.org/10.1093/jpids/piz023DOI Listing

Upsurge of Enterovirus D68 and Circulation of the New Subclade D3 and Subclade B3 in Beijing, China, 2016.

Sci Rep 2019 Apr 15;9(1):6073. Epub 2019 Apr 15.

School of Public Health, Capital Medical University, Beijing, 100069, P.R. China.

We conducted a surveillance among acute respiratory tract infection (ARTI) cases to define the epidemiology, clinical characteristics and genetic variations of enterovirus D68 (EV-D68) in Beijing, China from 2015 to 2017. Nasopharyngeal swabs and sputum were collected from 30 sentinel hospitals in Beijing and subjected to EV and EV-D68 detection by real-time PCR. The VP1 gene region and complete genome sequences of EV-D68 positive cases were analyzed. Read More

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http://dx.doi.org/10.1038/s41598-019-42651-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6465342PMC
April 2019
2 Reads

Automated cell-based luminescence assay for profiling antiviral compound activity against enteroviruses.

Sci Rep 2019 Apr 15;9(1):6023. Epub 2019 Apr 15.

Immunization Planning Institute, Henan Provincial Center for Disease Control and Prevention, Zhengzhou, Henan, People's Republic of China.

We describe the development, optimisation, and validation of an automated, cell-based and high-throughput screening assay using existing luminescence-based ATPlite reagents for identifying antiviral compounds that inhibit enterovirus replication. Antiviral efficacy was determined by measuring the ATP levels in cells that were protected from the viral cytopathic effect (CPE) by the antiviral compounds pleconaril and rupintrivir. CPE-based assay conditions were optimised at a cell density of 5000 cells/well and a viral infection dose of 100 CCID in 384-well plates. Read More

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http://www.nature.com/articles/s41598-019-42160-7
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http://dx.doi.org/10.1038/s41598-019-42160-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6465263PMC
April 2019
3 Reads

Enterovirus D68 Causing Acute Respiratory Infection: Clinical Characteristics and Differences With Acute Respiratory Infections Associated With Enterovirus Non-D68.

Pediatr Infect Dis J 2019 Apr 10. Epub 2019 Apr 10.

From the Microbiology Service, Hospital Universitario Donostia-Biodonostia Health Research Institute.

Background: Enterovirus (EV) D68 is mainly associated with acute respiratory infection (ARI). Since 2014, when outbreaks in different countries were observed, this emerging virus was considered a potential threat to public health.

Methods: During 2015-2017, the presence of enterovirus RNA was investigated in all respiratory samples of children younger than 15 years of age with ARI, obtained for virologic studies in the Pediatric Emergency Care Units and wards of 2 hospitals in Gipuzkoa (Spain), using a commercial multiplex real-time polymerase chain reaction. Read More

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http://dx.doi.org/10.1097/INF.0000000000002289DOI Listing
April 2019
1 Read

Incidence, Risk Factors and Outcomes Among Children With Acute Flaccid Myelitis: A Population-based Cohort Study in a California Health Network Between 2011 and 2016.

Pediatr Infect Dis J 2019 Apr 10. Epub 2019 Apr 10.

Department of Pediatric Infectious Diseases, Kaiser Permanente Oakland Medical Center, Oakland, California.

Background: Acute flaccid myelitis (AFM) is defined as an acute onset of limb weakness with longitudinal spinal gray matter lesions. Reporting bias and misdiagnosis confound epidemiologic studies of AFM. We mitigated these confounders by using a large data set to assess AFM incidence, risk factors and outcomes in a fixed population. Read More

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http://dx.doi.org/10.1097/INF.0000000000002276DOI Listing
April 2019
1 Read

Enterovirus 71 Suppresses miR-17-92 Cluster Through Up-Regulating Methylation of the miRNA Promoter.

Front Microbiol 2019 28;10:625. Epub 2019 Mar 28.

School of Life Sciences, Ningxia University, Yinchuan, China.

Enterovirus 71 (EV71), the etiological agent of hand-foot-and-mouth disease, has become an increasing public health challenge worldwide. Accumulating evidence suggests that mammalian microRNAs (miRNAs), a class of non-coding RNAs of 18 to 24 nucleotides (nt) with important regulatory roles in cellular processes, participate in host antiviral defense and studies have suggested roles of miRNAs in EV71 replication and pathogenesis. In the current study, we reported that the expression of hsa-miR-17∼92 cluster was significantly downregulated during EV71 infection. Read More

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https://www.frontiersin.org/article/10.3389/fmicb.2019.00625
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http://dx.doi.org/10.3389/fmicb.2019.00625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447709PMC
March 2019
2 Reads

Clinical characteristics and outcomes of patients with severe acute respiratory infections (SARI): results from the Egyptian surveillance study 2010-2014.

Multidiscip Respir Med 2019 1;14:11. Epub 2019 Apr 1.

Department of Clinical Pathology, Faculty of Medicine, Mansura University, Mansura, Egypt.

Background: Respiratory viral and atypical bacterial infections data in Egyptian patients are sparse. This study describes the clinical features and outcomes of patients with severe acute respiratory infections (SARI) in hospitalized patients in Egypt.

Methods: SARI surveillance was implemented at Cairo University Hospital (CUH) during the period 2010-2014. Read More

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http://dx.doi.org/10.1186/s40248-019-0174-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442424PMC
April 2019
1 Read

Biphasic and cardiomyocyte-specific IFIT activity protects cardiomyocytes from enteroviral infection.

PLoS Pathog 2019 Apr 8;15(4):e1007674. Epub 2019 Apr 8.

Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, California, United States of America.

Viral myocarditis is a serious disease, commonly caused by type B coxsackieviruses (CVB). Here we show that innate immune protection against CVB3 myocarditis requires the IFIT (IFN-induced with tetratricopeptide) locus, which acts in a biphasic manner. Using IFIT locus knockout (IFITKO) cardiomyocytes we show that, in the absence of the IFIT locus, viral replication is dramatically increased, indicating that constitutive IFIT expression suppresses CVB replication in this cell type. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453442PMC
April 2019
3 Reads

Persistence of Fecal Contamination Indicators and Pathogens in Class B Biosolids Applied to Sugarcane Fields.

J Environ Qual 2019 Mar;48(2):526-530

Agricultural recycling of human Class B biosolids in sugarcane ( spp.) crop is a promising alternative to reduce the costs of biosolids disposal. However, the presence of fecal contamination indicators such as thermotolerant coliforms and pathogenic organisms such as enterovirus and spp. Read More

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https://dl.sciencesocieties.org/publications/jeq/abstracts/4
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http://dx.doi.org/10.2134/jeq2018.07.0270DOI Listing
March 2019
4 Reads

High Prevalence of Viral Infections Among Hospitalized Pneumonia Patients in Equatorial Sarawak, Malaysia.

Open Forum Infect Dis 2019 Mar 13;6(3):ofz074. Epub 2019 Feb 13.

Duke Global Health Institute, Duke University, Durham, North Carolina.

Background: Although pneumonia is a known cause of morbidity and mortality in Sarawak, Malaysia, the etiology and epidemiology of pneumonia are not well described in this equatorial region. Routine clinical diagnostics for pneumonia etiology at government hospitals in Sarawak had historically involved only bacterial diagnostics. Viral diagnostics were only obtained through outside consultations. Read More

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https://academic.oup.com/ofid/article/doi/10.1093/ofid/ofz07
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http://dx.doi.org/10.1093/ofid/ofz074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440682PMC
March 2019
2 Reads

Spontaneous C-cleavage of a truncated intein as fusion tag to produce tag-free VP1 inclusion body nanoparticle vaccine against CVB3-induced viral myocarditis by the oral route.

Microb Cell Fact 2019 Apr 4;18(1):66. Epub 2019 Apr 4.

The Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou, 215123, Jiangsu, China.

Background: Oral vaccine is highly desired for infectious disease which is caused by pathogens infection through the mucosal surface. The design of suitable vaccine delivery system is ongoing for the antigen protection from the harsh gastric environment and target to the Peyer's patches to induce sufficient mucosal immune responses. Among various potential delivery systems, bacterial inclusion bodies have been widely used as delivery systems in the field of nanobiomedicine. Read More

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http://dx.doi.org/10.1186/s12934-019-1115-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449988PMC
April 2019
1 Read

Surveillance to Track Progress Toward Polio Eradication - Worldwide, 2017-2018.

MMWR Morb Mortal Wkly Rep 2019 Apr 5;68(13):312-318. Epub 2019 Apr 5.

When the Global Polio Eradication Initiative (GPEI) began in 1988, cases of poliomyelitis were reported from 125 countries. Since then, only Afghanistan, Nigeria, and Pakistan have experienced uninterrupted transmission of wild poliovirus (WPV). The primary means of detecting poliovirus is through surveillance for acute flaccid paralysis (AFP) among children aged <15 years with testing of stool specimens for WPV and vaccine-derived polioviruses (VDPVs) in World Health Organization (WHO)-accredited laboratories of the Global Polio Laboratory Network (GPLN) (1,2). Read More

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http://dx.doi.org/10.15585/mmwr.mm6813a4DOI Listing
April 2019
2 Reads

Molecular characteristic analysis for the VP1 region of coxsackievirus A6 strains isolated in Jiujiang area, China, from 2012 to 2013.

Medicine (Baltimore) 2019 Apr;98(14):e15077

Department of Histology and Embryology, Bengbu Medical College, Bengbu, Anhui, P.R. China.

Background: Enterovirus 71 (EV-A71) and Coxsackievirus A16 (CV-A16) are the most common causative agents causing hand, foot, and mouth disease (HFMD). However, coxsackievirus A6 (CV-A6), previously largely ignored, became the predominant pathogen in China in 2012. The objective of this study is to investigate the genetic characteristics and molecular epidemiology of HFMD caused by CV-A6 to guide the diagnosis and treatment of the disease, as well as disease prevention. Read More

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http://dx.doi.org/10.1097/MD.0000000000015077DOI Listing
April 2019
2 Reads

Immunohistochemical Study of 2 Cases of Coxsackie A6-Induced Atypical Hand-Foot-and-Mouth Disease.

Am J Dermatopathol 2019 Mar 20. Epub 2019 Mar 20.

Department of Dermatology, Fundación de Investigación Biomédica del Hospital Universitario de La Princesa, Madrid, Spain.

An atypical clinical variant of hand-foot-and-mouth disease (HFMD) with more extensive lesions and affecting adults has emerged during the past years, usually associated to the Coxsackievirus serotype A6 (CV-A6). We present a 19-year-old woman with a 3-day evolution eruption of papulovesicular lesions, which first appeared around the mouth and frontal area and rapidly spread. In addition, we present a 61-year-old man with a 4-day evolution asymptomatic eruption of papulovesicular lesions in both the hands and feet after suffering a cold 1 week before. Read More

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http://dx.doi.org/10.1097/DAD.0000000000001409DOI Listing
March 2019
4 Reads

Treatment of rituximab-associated chronic CNS enterovirus using IVIg and fluoxetine.

Neurology 2019 Apr 3. Epub 2019 Apr 3.

From the Departments of Pediatrics (L.S., A.B., S.D.D., R.S.M.Y., E.A.Y.), Medical Imaging (H.B.), Laboratory Medicine and Pathobiology (L.-N.H.), Immunology (R.S.M.Y.), and Medical Science (R.S.M.Y.), and Lawrence Bloomberg School of Nursing (J.J.), University of Toronto; the Divisions of Neurology (L.S., J.J., E.A.Y.), Infectious Diseases (A.B.), Diagnostic Imaging (H.B.), Pathology (L.-N.H.), Respirology (S.D.D.), and Rheumatology (R.S.M.Y.), and the Division of Neuroscience and Mental Health, Research Institute (E.A.Y.), The Hospital for Sick Children, Toronto, Canada.

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http://dx.doi.org/10.1212/WNL.0000000000007468DOI Listing
April 2019
5 Reads

Bacterial Stabilization of a Panel of Picornaviruses.

mSphere 2019 04 3;4(2). Epub 2019 Apr 3.

Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA

Several viruses encounter various bacterial species within the host and in the environment. Despite these close encounters, the effects of bacteria on picornaviruses are not completely understood. Previous work determined that poliovirus (PV), an enteric virus, has enhanced virion stability when exposed to bacteria or bacterial surface polysaccharides such as lipopolysaccharide. Read More

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http://dx.doi.org/10.1128/mSphere.00183-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449606PMC
April 2019
2 Reads

Distinct expression and clinical value of aquaporin 4 in children with hand, foot and mouth disease caused by enterovirus 71.

J Med Virol 2019 Apr 3. Epub 2019 Apr 3.

Department of Intensive Care Unit, Xuzhou Children's Hospital of Xuzhou Medical University, Xuzhou, China.

Brain dysfunction is a prerequisite for critical complications in children with hand, foot, and mouth disease (HFMD). Aquaporin 4 (AQP-4) may be involved in the pathological process of cerebral oedema and injury in children with severe and critical HFMD. This study aimed to assess the association of AQP-4 with the severity of enterovirus 71 (EV71)-associated HFMD. Read More

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http://dx.doi.org/10.1002/jmv.25475DOI Listing
April 2019
3 Reads

Acute Flaccid Myelitis: Something Old and Something New.

MBio 2019 04 2;10(2). Epub 2019 Apr 2.

National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA

Since 2014, acute flaccid myelitis (AFM), a long-recognized condition associated with polioviruses, nonpolio enteroviruses, and various other viral and nonviral causes, has been reemerging globally in epidemic form. This unanticipated reemergence is ironic, given that polioviruses, once the major causes of AFM, are now at the very threshold of global eradication and cannot therefore explain any aspect of AFM reemergence. Instead, the new AFM epidemic has been temporally associated with reemergences of nonpolio enteroviruses such as EV-D68, until recently thought to be an obscure virus of extremely low endemicity. Read More

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http://dx.doi.org/10.1128/mBio.00521-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445942PMC
April 2019
4 Reads

Discovery, Optimization, and Target Identification of Novel Potent Broad-Spectrum Antiviral Inhibitors.

J Med Chem 2019 Apr 12. Epub 2019 Apr 12.

College of Life Sciences , Hebei Normal University , Shijiazhuang , Hebei 050024 , P. R. China.

Viral infections are increasing and probably long-lasting global risks. In this study, a chemical library was exploited by phenotypic screening to discover new antiviral inhibitors. After optimizations from hit to lead, a novel potent small molecule (RYL-634) was identified, showing excellent broad-spectrum inhibition activity against various pathogenic viruses, including hepatitis C virus, dengue virus, Zika virus, chikungunya virus, enterovirus 71, human immunodeficiency virus, respiratory syncytial virus, and others. Read More

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http://dx.doi.org/10.1021/acs.jmedchem.9b00091DOI Listing
April 2019
5 Reads
5.447 Impact Factor

Role of innate lymphoid cells and dendritic cells in intradermal immunization of the enterovirus antigen.

NPJ Vaccines 2019 27;4:14. Epub 2019 Mar 27.

Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, 650118 Kunming, Yunnan China.

Enterovirus type 71 (EV71) and coxsackievirus A 16 (CA16) are the major pathogens of human hand, foot, and mouth disease (HFMD). In our previous study, intramuscular immunization with the inactivated EV71 vaccine elicited effective immunity, while immunization with the inactivated CA16 vaccine did not. In this report, we focused on innate immune responses elicited by inactivated EV71 and CA16 antigens administered intradermally or intramuscularly. Read More

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http://www.nature.com/articles/s41541-019-0108-6
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http://dx.doi.org/10.1038/s41541-019-0108-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437170PMC
March 2019
5 Reads

Cerebrospinal Fluid Neopterin in Children With Enterovirus-Related Brainstem Encephalitis.

Pediatr Neurol 2019 Feb 7. Epub 2019 Feb 7.

Department of Pediatrics, Hospital Sant Joan de Deu (University of Barcelona), Barcelona, Spain; Pediatric Infectious Diseases Research Group, Institut de Recerca Sant Joan de Deu, Barcelona, Spain; CIBER en Epidemiología y Salud Pública (CIBERESP), Madrid, Spain. Electronic address:

Background: Enterovirus-A71 causes outbreaks of brainstem encephalitis, ranging from self-limited disease to acute flaccid paralysis. The aim of this study was to assess the role of cerebrospinal fluid (CSF) neopterin as a biomarker of disease severity in children with enterovirus-related brainstem encephalitis.

Methods: A descriptive, prospective cohort study was conducted from April 2016 to March 2017 in a tertiary hospital. Read More

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http://dx.doi.org/10.1016/j.pediatrneurol.2019.01.024DOI Listing
February 2019
4 Reads

EpiRank: Modeling Bidirectional Disease Spread in Asymmetric Commuting Networks.

Sci Rep 2019 Apr 1;9(1):5415. Epub 2019 Apr 1.

Department of Computer Science and Engineering, National Taiwan Ocean University, Keelung City, 20224, Taiwan.

Commuting network flows are generally asymmetrical, with commuting behaviors bi-directionally balanced between home and work locations, and with weekday commutes providing many opportunities for the spread of infectious diseases via direct and indirect physical contact. The authors use a Markov chain model and PageRank-like algorithm to construct a novel algorithm called EpiRank to measure infection risk in a spatially confined commuting network on Taiwan island. Data from the country's 2000 census were used to map epidemic risk distribution as a commuting network function. Read More

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http://dx.doi.org/10.1038/s41598-019-41719-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443646PMC
April 2019
2 Reads

A decade of sustained selection pressure on two surface sites of the VP1 protein of Enterovirus A71 suggests that immune evasion may be an indirect driver for virulence.

Sci Rep 2019 Apr 1;9(1):5427. Epub 2019 Apr 1.

Division of Biomedical & Life Sciences, Faculty of Health & Medicine, Lancaster University, Lancaster, LA1 4YT, UK.

Enterovirus A71 (EV-A71) is an emerging pathogen in the Enterovirus A species group. EV-A71 causes hand, foot and mouth disease (HFMD), with virulent variants exhibiting polio-like acute flaccid paralysis and other central nervous system manifestations. We analysed all enterovirus A71 complete genomes with collection dates from 2008 to mid-2018. Read More

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http://www.nature.com/articles/s41598-019-41662-8
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http://dx.doi.org/10.1038/s41598-019-41662-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443798PMC
April 2019
3 Reads

Viruses with masked pathogenicity and genetically susceptible hosts-How to discover potentially pathogenic viruses.

J Med Virol 2019 Mar 30. Epub 2019 Mar 30.

Division of Host Defense, Research Center for the Prevention of Infectious Diseases, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

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http://dx.doi.org/10.1002/jmv.25472DOI Listing
March 2019
1 Read

Fatal Enteroviral Encephalitis in a Patient with Common Variable Immunodeficiency Harbouring a Novel Mutation in NFKB2.

J Clin Immunol 2019 Mar 29. Epub 2019 Mar 29.

Department of Clinical Immunology and Allergy, Royal Melbourne Hospital, Parkville, VIC, 3052, Australia.

Common variable immunodeficiency is the most prevalent of the primary immunodeficiency diseases, yet its pathogenesis is largely poorly understood. Of the cases that are monogenic, many arise due to pathogenic variants in NFKB1 and NFKB2. Here, we report enteroviral encephalomyelitis as the cause of a fatal neurodegenerative condition in a patient with a novel heterozygous mutation in NFKB2 (c. Read More

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http://dx.doi.org/10.1007/s10875-019-00602-xDOI Listing
March 2019
2 Reads

Phylogenetic analysis and phenotypic characterisatics of two Tibet EV-C96 strains.

Virol J 2019 03 29;16(1):40. Epub 2019 Mar 29.

WHO WPRO Regional Polio Reference Laboratory and NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

Background: Enterovirus C96 (EV-C96) is a newly named type of enterovirus belonging to species C, and the prototype strain (BAN00-10488) was firstly isolated in 2000 from a stool specimen of a patient with acute flaccid paralysis in Bangladesh. In this study, we report the genomic and phenotypic characteristics of two EV-C96 strains isolated from individuals from the Tibet Autonomous Region of China.

Methods: Human rhabdomyosarcoma (RD), human laryngeal epidermoid carcinoma (HEp-2), and human cervical cancer (Hela) cells were infected with the Tibet EV-C96 strains, and enterovirus RNA in the cell culture was detected with a real time RT-PCR-based enterovirus screening method. Read More

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http://dx.doi.org/10.1186/s12985-019-1151-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439968PMC
March 2019
2 Reads

Enterovirus D68-Associated Acute Respiratory Illness - New Vaccine Surveillance Network, United States, July-October, 2017 and 2018.

MMWR Morb Mortal Wkly Rep 2019 Mar 29;68(12):277-280. Epub 2019 Mar 29.

In the fall of 2014, an outbreak of enterovirus D68 (EV-D68)-associated acute respiratory illness (ARI) occurred in the United States (1,2); before 2014, EV-D68 was rarely reported to CDC (2,3). In the United States, reported EV-D68 detections typically peak during late summer and early fall (3). EV-D68 epidemiology is not fully understood because testing in clinical settings seldom has been available and detections are not notifiable to CDC. Read More

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http://dx.doi.org/10.15585/mmwr.mm6812a1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6448985PMC
March 2019
1 Read

Poliovirus Evolution toward Independence from the Phosphatidylinositol-4 Kinase III β/Oxysterol-Binding Protein Family I Pathway.

ACS Infect Dis 2019 Apr 5. Epub 2019 Apr 5.

Université Côte d'Azur, CNRS , Institut de Pharmacologie Moléculaire et Cellulaire , 660 route des lucioles , Valbonne 06560 , France.

Phosphatidylinositol-4 kinase III β (PI4KB) and oxysterol-binding protein (OSBP) family I provide a conserved host pathway required for enterovirus replication. Here, we analyze the role and essentiality of this pathway in enterovirus replication. Phosphatidylinositol 4-phosphate (PI4P) production and cholesterol accumulation in the replication organelle (RO) are severely suppressed in cells infected with a poliovirus (PV) mutant isolated from a PI4KB-knockout cell line (RD[Δ PI4KB]). Read More

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http://dx.doi.org/10.1021/acsinfecdis.9b00038DOI Listing
April 2019
4 Reads

Clinical characteristics and managements of severe hand, foot and mouth disease caused by enterovirus A71 and coxsackievirus A16 in Shanghai, China.

BMC Infect Dis 2019 Mar 27;19(1):285. Epub 2019 Mar 27.

Department of Pediatrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai, 200092, China.

Background: Hand, foot and mouth disease (HFMD) is a transmissible infectious disease caused by human enteroviruses (EV). Here, we described features of children with severe HFMD caused by EV-A71 or coxsackievirus A16 (CV-A16) in Shanghai, China.

Methods: Severe EV-A71 or CV-A16 caused HFMD children admitted to the Xinhua Hospital from January 2014 and December 2016, were recruited retrospectively to the study. Read More

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http://dx.doi.org/10.1186/s12879-019-3878-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438032PMC
March 2019
1 Read

Characterization of lymphocyte subsets in peripheral blood cells of children with EV71 infection.

J Microbiol Immunol Infect 2019 Mar 14. Epub 2019 Mar 14.

Virus Laboratory, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 318 Renminzhong Road Yuexiu District, Guangzhou, 510120, China. Electronic address:

Background: Enterovirus 71 (EV71) is one of the major causative pathogens of hand, foot, and mouth disease (HFMD). Immune cells play a critical role in determining the outcomes of virus infection. We aimed to characterize the lymphocyte subsets and transcriptional levels of T lymphocytes-associated transcription factors in peripheral blood cells of children with EV71 infection. Read More

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http://dx.doi.org/10.1016/j.jmii.2019.03.001DOI Listing
March 2019
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Update on association between Kawasaki disease and infection.

J Chin Med Assoc 2019 Mar;82(3):172-174

Department of Pediatrics, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, ROC.

The relationship between infection and Kawasaki disease (KD) remains unclear. Infection has long been considered a key predisposing factor for KD. Bacterial and viral agents may be related to the onset of KD because of superantigen and cytokine production. Read More

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http://dx.doi.org/10.1097/JCMA.0000000000000025DOI Listing
March 2019
3 Reads

Discovery of Quinoline Analogues as Potent Antivirals against Enterovirus D68 (EV-D68).

J Med Chem 2019 Apr 3. Epub 2019 Apr 3.

Enterovirus D68 (EV-D68) is an atypical nonpolio enterovirus that mainly infects the respiratory system of humans, leading to moderate-to-severe respiratory diseases. In rare cases, EV-D68 can spread to the central nervous system and cause paralysis in infected patients, especially young children and immunocompromised individuals. There is currently no approved vaccine or antiviral available for the prevention and treatment of EV-D68. Read More

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http://pubs.acs.org/doi/10.1021/acs.jmedchem.9b00115
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http://dx.doi.org/10.1021/acs.jmedchem.9b00115DOI Listing
April 2019
17 Reads
5.447 Impact Factor

Autophagy induced by enterovirus 71 regulates the production of IL-6 through the p38MAPK and ERK signaling pathways.

Microb Pathog 2019 Mar 23;131:120-127. Epub 2019 Mar 23.

Fenyang College of Shanxi Medical University, Fenyang, China; Graduate School of Shanxi Medical University, Taiyuan, China. Electronic address:

Enterovirus 71 (EV71) is the main causative agent of hand, foot, and mouth disease (HFMD), which has high morbidity and mortality. It mainly threatens children under six years of age. Because of a poor understanding of its pathogenesis, there are no effective drugs to control EV71 infection. Read More

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http://dx.doi.org/10.1016/j.micpath.2019.03.028DOI Listing
March 2019
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Characteristics of enterovirus 71-induced cell death and genome scanning to identify viral genes involved in virus-induced cell apoptosis.

Virus Res 2019 May 22;265:104-114. Epub 2019 Mar 22.

Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College of Fudan University, Shanghai 200032, China; Laboratory of Medical Microbiology, Department of Medical Microbiology and Parasitology, Shanghai Medical College of Fudan University, 138 Yixueyuan R., Shanghai 200032, China. Electronic address:

Enterovirus 71 (EV71) causes hand-foot-and-mouth disease and severe neural complications in infants and young children. Viral pathogenesis is associated with virus-induced cell death and inflammatory cytokine production, which is usually correlated with the type of programmed cell death. EV71-infected cells were analyzed through microscopy, cell staining, and immunoblotting to determine the characteristics of EV71-induced cell death. Read More

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http://dx.doi.org/10.1016/j.virusres.2019.03.017DOI Listing
May 2019
2 Reads

Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia.

J Virol Methods 2019 Mar 22;269:1-6. Epub 2019 Mar 22.

Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. Electronic address:

Background: The role of respiratory viruses as the major cause of acute lower respiratory tract infections (ALRTIs) in children is becoming increasingly evident due to the use of sensitive molecular detection methods. The aim of this study was to use conventional and molecular detection methods to assess the epidemiology of respiratory viral infections in children less than five years of age that were hospitalized with ALRTIs.

Methods: The cross-sectional study was designed to investigate the occurrence of respiratory viruses including respiratory syncytisl virus (RSV), human metapneumovirus (HMPV), influenza virus A and B (IFV-A and B), parainfluenzavirus 1, 2, 3 and 4 (PIV 1, 2, 3 and 4), human rhinoviruses (HRV), human enterovirus (HEV), human coronaviruses (HCoV) 229E and OC43, human bocavirus (HBoV) and human adenovirus (HAdV) in hospitalized children with ALRTIs, at Hospital Serdang, Malaysia, from June 16 to December 21, 2009. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.03.013DOI Listing
March 2019
6 Reads
1.883 Impact Factor

Compounds based on 5-(perylen-3-ylethynyl)uracil scaffold: High activity against tick-borne encephalitis virus and non-specific activity against enterovirus A.

Eur J Med Chem 2019 Jun 16;171:93-103. Epub 2019 Mar 16.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, Moscow 117997, Russia. Electronic address:

Rigid amphipathic fusion inhibitors (RAFIs) are potent antivirals based on a perylene core linked with a nucleoside moiety. Sugar-free analogues of RAFIs, 5-(perylen-3-ylethynyl)uracil-1-acetic acid 1 and its amides 2, were synthesized using combined protection group strategy. Compounds 1 and 2 appeared to have low toxicity on porcine embryo kidney (PEK) or rhabdomiosarcoma (RD) cells together with remarkable activity against enveloped tick-borne encephalitis virus (TBEV): EC values vary from 0. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S02235234193024
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http://dx.doi.org/10.1016/j.ejmech.2019.03.029DOI Listing
June 2019
7 Reads

Neurodevelopment medium-term outcome after parechovirus infection.

Early Hum Dev 2019 Mar 22;132:1-5. Epub 2019 Mar 22.

Department of Pediatrics and Infectious Diseases, Hospital Universitario La Paz, Madrid, Spain; Fundación IdiPaz, Spain; Red de Investigación Traslacional en Infectología Pediátrica (RITIP), Spain; TEDDY Network, European Network of Excellence for Pediatric Clinical Research, Italy.

Aim: Human parechoviruses (HPeV) are responsible for fever without a source (FWS), sepsis-like illness and encephalitis in neonates and children under 3 months of age. Short-term outcome is generally good, but there is great concern about medium and long- term outcome of infants after HPeV infection. The aim of this study is to assess the medium-term outcome in infants following HPeV infection without encephalitis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03783782183065
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http://dx.doi.org/10.1016/j.earlhumdev.2019.03.005DOI Listing
March 2019
5 Reads

An antiviral drug screening system for enterovirus 71 based on an improved plaque assay: A potential high-throughput method.

J Med Virol 2019 Mar 22. Epub 2019 Mar 22.

Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, China.

Plaque assay plays an irreplaceable role in a variety of virological studies, including determining titers of viruses. Our previous study showed that a simple and highly repeatable plaque assay could be used for enterovirus 71 (EV-A71). Now, we show that using a subclone of a clinical EV-A71 isolate and a rhabdomyosarcoma cell line (RD), a plaque assay based on an EV-A71/RD model could exhibit the most rapid formation of plaques (<2 days), with much higher repeatability and consistency. Read More

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http://dx.doi.org/10.1002/jmv.25463DOI Listing
March 2019
9 Reads
2.347 Impact Factor

Incidence and seasonality of respiratory viruses causing acute respiratory infections in the Northern United Arab Emirates.

J Med Virol 2019 Mar 22. Epub 2019 Mar 22.

Department of Laboratory Medicine, Sheikh Khalifa Specialty Hospital, Ras Al Khaimah, UAE.

Background: The data on the seasonality of respiratory viruses helps to ensure the optimal vaccination period and to monitor the possible outbreaks of variant type.

Objectives: This study was designed to describe the molecular epidemiology and seasonality of acute respiratory infection (ARI)-related respiratory viruses in the United Arab Emirates (UAE).

Methods: Both upper and lower respiratory specimens were collected for the analysis from all the patients who visited the Sheikh Khalifa Specialty Hospital (SKSH) with ARI for over 2 years. Read More

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http://dx.doi.org/10.1002/jmv.25464DOI Listing
March 2019
3 Reads

'Don't put your head under water': enteric viruses in Brazilian recreational waters.

New Microbes New Infect 2019 May 14;29:100519. Epub 2019 Feb 14.

Laboratório de Saúde Única, Universidade Feevale, Novo Hamburgo, Brazil.

Like in many other countries, virologic analyses are not routinely performed in Brazil in monitoring water quality for recreational purposes. We surveyed current research regarding viral contamination of recreational water environments in Brazil. Among the enteric viruses studied in Brazilian recreational waters, we highlight adenoviruses, rotaviruses, enteroviruses and noroviruses. Read More

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http://dx.doi.org/10.1016/j.nmni.2019.100519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406054PMC
May 2019
1 Read

Early-life exposure to common virus infections did not differ between coeliac disease patients and controls.

Acta Paediatr 2019 Mar 21. Epub 2019 Mar 21.

Department of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.

Aim: Our aim was to compare the presence of various common viruses (rhinovirus, enterovirus, adenovirus, Epstein-Barr virus, cytomegalovirus, norovirus, parechovirus) in stool and nasal swab samples as well as virus-specific antibodies in serum samples between children who developed coeliac disease and controls.

Methods: A case-control study was established based on the DIABIMMUNE Study cohorts. During the study, eight Estonian children and 21 Finnish children aged 1. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/apa.14791
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http://dx.doi.org/10.1111/apa.14791DOI Listing
March 2019
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Environmental Surveillance of Human Enteroviruses in Córdoba City, Argentina: Prevalence and Detection of Serotypes from 2009 to 2014.

Food Environ Virol 2019 Mar 20. Epub 2019 Mar 20.

Facultad de Ciencias Médicas, Instituto de Virología "Dr. J. M. Vanella", Universidad Nacional de Córdoba, Enfermera Gordillo Gómez s/n Ciudad Universitaria, CP: 5016, Córdoba, Argentina.

Environmental surveillance is an effective approach to investigate the circulation of human enteroviruses (EVs) in the population. EVs excreted by patients who present diverse clinical syndromes can remain infectious in the environment for several weeks, and limited data on circulating environmental EVs are available. A 6-year (2009-2014) surveillance study was conducted to detect non-polio enteroviruses (NPEVs) in the urban sewage of Cordoba city, Argentina. Read More

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http://dx.doi.org/10.1007/s12560-019-09379-zDOI Listing
March 2019
2 Reads

Immunogenicity and safety of an inactivated enterovirus 71 vaccine administered simultaneously with recombinant hepatitis B vaccine and group A meningococcal polysaccharide vaccine:  a phase IV, open-label, single-center, randomized, non-inferiority trial.

J Infect Dis 2019 Mar 19. Epub 2019 Mar 19.

Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, Guangdong, China.

Background: This study tested the hypothesis that the immunogenicity and safety of the simultaneous administration of the combined EV71 vaccine with recombinant hepatitis B vaccine (HepB) and group A meningococcal polysaccharide vaccine (MenA) is not inferior to separate administration of each vaccine.

Methods: The study was designed as a randomized, open-label, and non-inferiority trial, and was registered at ClinicalTrials.gov (NCT03274102). Read More

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http://dx.doi.org/10.1093/infdis/jiz129DOI Listing
March 2019
5 Reads

Acute Flaccid Myelitis: Lessons From Polio.

Authors:
Janet R Gilsdorf

J Pediatric Infect Dis Soc 2019 Mar 19. Epub 2019 Mar 19.

Department of Pediatrics, University of Michigan Medical School, Ann Arbor.

With the eradication of poliomyelitis in the United States, the appearance of acute flaccid myelitis outbreaks has raised questions regarding their causation. Review of the epidemiology, clinical aspects, and laboratory findings of bygone cases of poliomyelitis have revealed shows important similarities with those of newer cases of acute flaccid myelitis. Many occurrences are preceded by an apparent viral illness, and a number of viruses, particularly enteroviruses A71 and D68, can be isolated from respiratory or stool specimens. Read More

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http://dx.doi.org/10.1093/jpids/piz017DOI Listing
March 2019
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Ferritin nanocage-based antigen delivery nanoplatforms: epitope engineering for peptide vaccine design.

Biomater Sci 2019 Mar 19. Epub 2019 Mar 19.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China. and National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University, Xiamen, 361102, China.

Biomedical applications and nanotechnological advances, including constrained synthesis, multimodal imaging, drug delivery, and bioassay, have strongly benefited from employing ferritin nanocages due to their remarkable properties of easy engineering, great biocompatible features, large capacity and so on. In this study, ferritin nanocages were used to display epitopes (model antigens derived from Enterovirus 71 (EV71) with different length) on C- and N-terminals and the loop zone to search for the optimal position for the fusion of the epitopes to the vaccine platform. The longest epitope displayed on the N-terminal and loop zone as well as the second longest peptide displayed on the loop zone of ferritin resulted in 100% passive protection of newborn BALB/c mice from the lethal EV71. Read More

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http://dx.doi.org/10.1039/c9bm00098dDOI Listing
March 2019
1 Read