2,173 results match your criteria Connective Tissue Research [Journal]


Rapamycin attenuates articular cartilage degeneration by inhibiting β-catenin in a murine model of osteoarthritis.

Connect Tissue Res 2019 Feb 20. Epub 2019 Feb 20.

b Orthopedics Ward 3, The General Hospital of Ningxia Medical University , Yinchuan , Ningxia 750004 , P.R. China.

To investigate whether systemic injection of rapamycin attenuates articular cartilage degeneration by inhibiting β-catenin in a murine model of osteoarthritis (OA). Ten-week-old male C57BL/6j wild-type (WT) mice and SOST-knockout (SOST) mice were randomized to a sham group, a vehicle-treated group, and a rapamycin-treated group. Mice in the vehicle-treated group underwent destabilizing of the medial meniscus (DMM) in the right knee, and were then treated with vehicle. Read More

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http://dx.doi.org/10.1080/03008207.2019.1583223DOI Listing
February 2019

N-acetylcysteine Reduces Glutamate-Induced Cytotoxicity to Fibroblasts of Rat Supraspinatus Tendons.

Connect Tissue Res 2019 Feb 12. Epub 2019 Feb 12.

d Department of Orthopaedic Surgery , School of Medicine and Gyeongsang National University Changwon Hospital , Changwon , Republic of Korea.

Purpose: Neuronal theory regarding rotator cuff degeneration has developed from the findings that glutamate, an amino acid and an excitatory neurotransmitter, is present in increased concentrations in tendon tissues with tendinopathy and that glutamate induces cell death in fibroblasts of origin in rat supraspinatus tendon. The purpose of the current study was to determine whether N-acetylcysteine (NAC) has cytoprotective effects against glutamate-induced fibroblast death.

Materials And Methods: Primary cultured fibroblasts were obtained from rat supraspinatus tendons. Read More

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http://dx.doi.org/10.1080/03008207.2019.1580702DOI Listing
February 2019
1 Read

The effect of CPNE7 on periodontal regeneration.

Connect Tissue Res 2019 Feb 8:1-12. Epub 2019 Feb 8.

b Laboratory for the Study of Regenerative Dental Medicine, Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute , Seoul National University , Seoul , Republic of Korea.

Introduction: Preameloblast-conditioned medium (PA-CM), as a mixture of dental epithelium-derived factors, has been reported to regenerate dentin and periodontal tissues in vitro and in vivo. The aim of this study was to investigate the biological effect of Cpne7 on the proliferation, migration, and cementoblast differentiation of periodontal cells in vitro, and on the regeneration of periodontal tissue using periodontal defect model with canine in vivo.

Materials And Methods: The effect of Cpne7 on cell proliferation, migration, and cementoblast differentiation of periodontal cells were evaluated in vitro. Read More

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http://dx.doi.org/10.1080/03008207.2019.1574776DOI Listing
February 2019
1 Read

Diverticulosis and nine connective tissue disorders: epidemiological support for an association.

Connect Tissue Res 2019 Feb 5:1-10. Epub 2019 Feb 5.

f Waitemata District Health Board , University of Auckland, and Geriatrician , Auckland , New Zealand.

Purpose: An underlying connective tissue disorder (CTD) may predispose to formation of intestinal diverticula. We assess the association of diverticulosis with nine selected CTDs, to inform the pathophysiology of diverticula.

Methods: A population-based period-prevalence study. Read More

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http://dx.doi.org/10.1080/03008207.2019.1570169DOI Listing
February 2019
1 Read

Fibrosis in distinct tissues.

Authors:
Andrzej Fertala

Connect Tissue Res 2019 Jan;60(1):1-2

a Department of Orthopaedic Surgery , Thomas Jefferson University , Philadelphia , PA , USA.

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https://www.tandfonline.com/doi/full/10.1080/03008207.2019.1
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http://dx.doi.org/10.1080/03008207.2019.1554755DOI Listing
January 2019
2 Reads

Estimation of the elastic modulus of child cortical bone specimens via microindentation.

Connect Tissue Res 2019 Feb 5:1-7. Epub 2019 Feb 5.

a Aix Marseille Univ, CNRS, ISM, Inst Movement Sci , Marseille , France.

Purpose: Non-pathological child cortical bone (NPCCB) studies can provide clinicians with vital information and insights. However, assessing the anisotropic elastic properties of NPCCB remains a challenge for the biomechanical engineering community. For the first time, this paper provides elastic moduli values for NPCCB specimens in two perpendicular directions (longitudinal and transverse) and for two different structural components of bone tissue (osteon and interstitial lamellae). Read More

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https://www.tandfonline.com/doi/full/10.1080/03008207.2019.1
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http://dx.doi.org/10.1080/03008207.2019.1570170DOI Listing
February 2019
4 Reads

Transforming Growth Factor-β1 Stimulates Mesenchymal Stem Cells Proliferation via Altering Cell Cycle through FAK-Akt-mTOR Pathway.

Connect Tissue Res 2019 Jan 15. Epub 2019 Jan 15.

a State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai 200237 , P. R. China.

Background: Mesenchymal stem cells (MSCs) are promising for cell therapy and regenerative medicine. An increased need for expanding of MSCs under serum-free condition to achieve a sufficient quantity for therapeutic applications is inevitable. Transforming growth factor-β1 (TGF-β1) is widely used for expanding clinical-grade MSCs in vitro. Read More

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https://www.tandfonline.com/doi/full/10.1080/03008207.2019.1
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http://dx.doi.org/10.1080/03008207.2019.1570171DOI Listing
January 2019
4 Reads

Interleukin-35 suppresses antitumor activity of circulating CD8 T cells in osteosarcoma patients.

Connect Tissue Res 2019 Jan 7:1-9. Epub 2019 Jan 7.

a Department of Orthopaedic Traumatology , The First Hospital of Jilin University , Changchun , China.

Purpose/Aim of the study: Interleukin (IL)-35 is a newly identified IL-12 cytokine family member and reveals immunosuppressive activity to CD8 T cells in inflammation, infectious diseases, and cancers. However, little is known regarding IL-35 function in osteosarcoma. Thus, the aim of the current study was to investigate the regulatory function of IL-35 to CD8+ T cells in osteosarcoma. Read More

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https://www.tandfonline.com/doi/full/10.1080/03008207.2018.1
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http://dx.doi.org/10.1080/03008207.2018.1552267DOI Listing
January 2019
2 Reads
1.607 Impact Factor

3D printing with silk: considerations and applications.

Connect Tissue Res 2018 Dec 17:1-11. Epub 2018 Dec 17.

b Biomedical Engineering Department , Carnegie Mellon University , Pittsburgh , PA , USA.

Silk is a natural polymer sourced mainly from spiders and silkworms. Due to its biocompatibility, biodegradability, and mechanical properties, it has been heavily investigated for biomedical applications. It can be processed into a number of formats, such as scaffolds, films, and nanoparticles. Read More

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https://www.tandfonline.com/doi/full/10.1080/03008207.2018.1
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http://dx.doi.org/10.1080/03008207.2018.1553959DOI Listing
December 2018
3 Reads

Bio-ink development for three-dimensional bioprinting of hetero-cellular cartilage constructs.

Connect Tissue Res 2018 Dec 10:1-15. Epub 2018 Dec 10.

a Department of Orthopaedics, University Medical Center Utrecht , Utrecht University , Utrecht , The Netherlands.

Bioprinting is a promising tool to fabricate organized cartilage. This study aimed to investigate the printability of gelatin-methacryloyl/gellan gum (gelMA/gellan) hydrogels with and without methacrylated hyaluronic acid (HAMA), and to explore (zone-specific) chondrogenesis of chondrocytes, articular cartilage progenitor cells (ACPCs), and multipotent mesenchymal stromal cells (MSCs) embedded in these bio-inks. The incorporating of HAMA in gelMA/gellan bio-ink increased filament stability, as measured using a filament collapse assay, but did not influence (zone-specific) chondrogenesis of any of the cell types. Read More

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http://dx.doi.org/10.1080/03008207.2018.1553960DOI Listing
December 2018
1 Read

Proliferation of rheumatoid arthritis fibroblast-like synoviocytes is enhanced by IL-17-mediated autophagy through STAT3 activation.

Connect Tissue Res 2018 Nov 26. Epub 2018 Nov 26.

a Department of Rheumatoid Immunity , the First Affiliated Hospital of Jinzhou Medical University , Jinzhou , Liaoning , 121000 , China .

Fibroblast-like synoviocytes (FLSs), with their tumor-like proliferation, play an important role in rheumatoid arthritis (RA), and interleukin-17 (IL-17) participates in RA pathology by affecting FLSs. The aims of this study were to investigate the effects of IL-17 on the proliferation and autophagy of FLSs and the role of signal transducer and activator of transcription-3 (STAT3) in RA. FLSs were treated with IL-17 at different concentrations (0, 1, 10, and 20 ng/mL); then, autophagy was assayed with western blotting, immunofluorescence and transmission electron microscopy. Read More

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https://www.tandfonline.com/doi/full/10.1080/03008207.2018.1
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http://dx.doi.org/10.1080/03008207.2018.1552266DOI Listing
November 2018
12 Reads

Bay11-7082 facilitates wound healing by antagonizing mechanical injury- and TNF-α-induced expression of MMPs in posterior cruciate ligament.

Connect Tissue Res 2018 Oct 29:1-12. Epub 2018 Oct 29.

a "111" project Laboratory of Biomechanics and Tissue Repair, Bioengineering College , Chongqing University , Chongqing , China.

Purposes: To investigate the ability of synoviocytes (SCs) in regulating MMPs expression in the posterior cruciate ligament fibroblasts (PCLfs) after TNF-α treatment, to test whether a specific inflammation inhibitor Bay11-7082 can antagonize the expression of MMPs in PCLfs after injury.

Methods: The microenvironment of knee joint cavity after PCL injury was mimicked in an in vitro co-culture system. The effects of TNF-α treatment on the expression of MMPs in PCL fibroblasts (PCLfs) were studied. Read More

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https://www.tandfonline.com/doi/full/10.1080/03008207.2018.1
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http://dx.doi.org/10.1080/03008207.2018.1512978DOI Listing
October 2018
6 Reads
1.610 Impact Factor

Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393.

Connect Tissue Res 2018 Oct 22. Epub 2018 Oct 22.

b Institute of Anatomy , Paracelsus Medical University , Salzburg and Nuremberg , Germany.

Damage of hyaline cartilage such as nasoseptal cartilage requires proper reconstruction, which remains challenging due to its low intrinsic repair capacity. Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair. Despite so far mostly tested for bone tissue engineering, bioactive glass (BG) could exert stimulatory effects on chondrogenesis. Read More

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http://dx.doi.org/10.1080/03008207.2018.1539083DOI Listing
October 2018
3 Reads

Single vs. repeated matrix metalloproteinase-13 knockdown with intra-articular short interfering RNA administration in a murine osteoarthritis model.

Connect Tissue Res 2018 Oct 21:1-9. Epub 2018 Oct 21.

a Department of Orthopaedic Surgery , Graduate School of Medical and Sciences, Chiba University , Chiba , Japan.

Purpose: Our aims were 1) to estimate the duration of short interfering RNA (siRNA) effect on matrix metalloproteinase-13 (Mmp-13) levels by a single intra-articular injection using a mouse knee osteoarthritis (OA) model and 2) to test whether repeated injections results in any additional suppressive effect on cartilage degradation compared to a single injection.

Materials And Methods: OA was induced in 9 weeks old male C57BL/6 mice by destabilization of medial meniscus (DMM). Chemically modified siRNA targeted for Mmp-13 was injected into the knee joint at 1 week post-DMM surgery. Read More

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http://dx.doi.org/10.1080/03008207.2018.1539082DOI Listing
October 2018
18 Reads

Fine-tuning the ubiquitin-proteasome system to treat pulmonary fibrosis.

Connect Tissue Res 2019 Jan 22;60(1):50-61. Epub 2018 Oct 22.

a Center for Translational Medicine and Jane and Leonard Korman Lung Center , Thomas Jefferson University , Philadelphia , PA , USA.

Idiopathic pulmonary fibrosis (IPF) is an extremely aggressive lung disease that develops almost exclusively in older individuals, carries a very poor prognosis, and lacks any truly effective therapies. The current conceptual model is that IPF develops because of an age-related decline in the ability of the lung epithelium to regenerate after injury, largely due to death or senescence of epithelial progenitor cells in the distal airways. This loss of regenerative capacity is thought to initiate a chronic and ineffective wound-healing response, characterized by persistent, low-grade lung inflammation and sustained production of collagen and other extracellular matrix materials. Read More

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http://dx.doi.org/10.1080/03008207.2018.1529174DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373466PMC
January 2019
13 Reads

Multiscale molecular interactions of tendon.

Authors:
Dianne Little

Connect Tissue Res 2018 Sep;59(5):393-395

a Department of Basic Medical Sciences and Department of Biomedical Engineering , Purdue University , West Lafayette , IN , USA.

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https://www.tandfonline.com/doi/full/10.1080/03008207.2018.1
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http://dx.doi.org/10.1080/03008207.2018.1514801DOI Listing
September 2018
2 Reads

Inhibition of Nf-ҝb prevents trauma-induced heterotopic ossification in rat model.

Connect Tissue Res 2018 Oct 10:1-7. Epub 2018 Oct 10.

b Department of Orthopedics , Shanghai Fenxian District Central Hospital , Shanghai , China.

Purpose: To investigate the pathogenesis and find a better prophylactic method of acquired heterotopic ossification (HO).

Materials And Methods: In the first part, we designed the brain-traumatic/burn/tenotomy rat model and testified its efficacy as HO model. 44 rats were randomly divided into experimental group and control group. Read More

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http://dx.doi.org/10.1080/03008207.2018.1530771DOI Listing
October 2018
5 Reads

Reactive oxygen species are responsible for the cell aggregation and production of pro-inflammatory mediators in phorbol ester (PMA)-treated U937 cells on gelatin-coated dishes through upregulation of autophagy.

Connect Tissue Res 2018 Oct 2. Epub 2018 Oct 2.

a China-Japan Research Institute of Medical and Pharmaceutical Sciences , Shenyang Pharmaceutical University , Shenyang 110016 , China.

Purpose: Our previous studies indicate that phorbol 12-myristate 13-acetate (PMA)-treated U937 cells cultured on collagen I -coated dishes express lowered production of pro-inflammatory mediators in parallel through reduced Reactive oxygen species (ROS) levels. By contrast, PMA-treated U937 cells on gelatin, the denatured collagen, show enhanced production of pro-inflammatory mediators, mediated by up-regulating autophagy levels. The present study is aimed to investigate the effect of ROS levels in PMA-treated U937 cells cultured on gelatin-coated surface. Read More

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https://www.tandfonline.com/doi/full/10.1080/03008207.2018.1
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http://dx.doi.org/10.1080/03008207.2018.1530770DOI Listing
October 2018
6 Reads

Roadmap of molecular, compositional, and functional markers during embryonic tendon development.

Connect Tissue Res 2018 Sep 20;59(5):495-508. Epub 2018 Sep 20.

a Department of Biomedical Engineering , University of Rochester , Rochester , NY , USA.

Tendon is a specialized connective tissue that connects muscle to bone, thereby enabling musculoskeletal movement. Tendon injury leads to formation of tissue with aberrant functional properties. Current approaches to treat tendon injuries, including surgical repair and tissue engineering, have not achieved normal tendon. Read More

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http://dx.doi.org/10.1080/03008207.2018.1511710DOI Listing
September 2018
5 Reads

Cellular, matrix, and mechano-biological differences in load-bearing versus positional tendons throughout development and aging: a narrative review.

Connect Tissue Res 2018 Sep 20;59(5):483-494. Epub 2018 Sep 20.

a Raymond Purves Bone and Joint Research Laboratories, Institute of Bone and Joint Research and Kolling Institute, Sydney Medical School , University of Sydney, at Royal North Shore Hospital , St Leonards , Australia.

Purpose: Summarise available evidence comparing the cellular, biochemical, structural and biomechanical properties, and the changes that occur in these parameters in response to stimuli, in differentially loaded tendons across different stages of life.

Methods: The PubMed database was searched for literature pertaining to differences between tendons using the term "tendon" or "tendinopathy", plus one or more of the following descriptors: "loading", "positional", "weight- or load-bearing", and "energy-storing". The abstracts were reviewed and relevant full-length articles retrieved and used to assemble a narrative review. Read More

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http://dx.doi.org/10.1080/03008207.2018.1504929DOI Listing
September 2018
2 Reads

Fibroblasts from recurrent fibrotic overgrowths reveal high rate of proliferation in vitro - findings from the study of hereditary and idiopathic gingival fibromatosis.

Connect Tissue Res 2019 Jan 20;60(1):29-39. Epub 2018 Sep 20.

b Department of Periodontology and Oral Medicine, Medical College , Jagiellonian University , Krakow , Poland.

Purpose: Investigate the content of fibrotic fibrils in gingival tissue and the proliferation of fibroblasts collected from recurrent and non-recurrent hereditary gingival fibromatosis (HGF) and idiopathic gingival fibromatosis (IGF).

Methods: Gingival biopsies were collected from HGF (n = 3) and IGF (n = 3) donors with recurrent and non-recurrent gingival overgrowths and from a control group (Ctrl, n = 3). Hematoxylin staining was performed to evaluate the histomorphology of gingival tissue. Read More

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http://dx.doi.org/10.1080/03008207.2018.1517758DOI Listing
January 2019
14 Reads

Peripheral nerve injury, scarring, and recovery.

Connect Tissue Res 2019 Jan 6;60(1):3-9. Epub 2018 Sep 6.

a Department of Orthopaedic Surgery, Sidney Kimmel Medical School , Thomas Jefferson University , Philadelphia , PA , USA.

Peripheral nerve injuries (PNI) resulting from trauma can be severe and permanently debilitating. Despite the armamentarium of meticulous microsurgical repair techniques that includes direct repair, grafting of defects with autograft nerve, and grafting with cadaveric allografts, approximately one-third of all PNI demonstrate incomplete recovery with poor restoration of function. This may include total loss or incomplete recovery of motor and/or sensory function, chronic pain, muscle atrophy, and profound weakness, which can result in lifelong morbidity. Read More

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http://dx.doi.org/10.1080/03008207.2018.1489381DOI Listing
January 2019
1 Read

Arthrofibrosis and large joint scarring.

Connect Tissue Res 2019 Jan 20;60(1):21-28. Epub 2018 Sep 20.

d Department of Orthopaedics , Rothman Institute at Thomas Jefferson University , Philadelphia , PA , USA.

Large joint arthrofibrosis and scarring, involving the shoulder, elbow, hip, and knee, can result in the loss of function and immobility. The pathway of joint contracture formation is still being elucidated and is due to aberrations in collagen synthesis and misorientation of collagen fibrils. Novel antibodies are being developed to prevent arthrofibrosis, and current treatment methods for arthrofibrosis include medical, physical, and surgical treatments. Read More

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http://dx.doi.org/10.1080/03008207.2018.1517759DOI Listing
January 2019
10 Reads

Biologic and mechanical aspects of tendon fibrosis after injury and repair.

Connect Tissue Res 2019 Jan 7;60(1):10-20. Epub 2018 Sep 7.

a Department of Orthopaedic Surgery, Sidney Kimmel Medical School , Thomas Jefferson University , Philadelphia , PA , USA.

Tendon injuries of the hand that require surgical repair often heal with excess scarring and adhesions to adjacent tissues. This can compromise the natural gliding mechanics of the flexor tendons in particular, which operate within a fibro-osseous tunnel system similar to a set of pulleys. Even combining the finest suture repair techniques with optimal hand therapy protocols cannot ensure predictable restoration of hand function in these cases. Read More

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http://dx.doi.org/10.1080/03008207.2018.1512979DOI Listing
January 2019
23 Reads

Intra-articular treatment with corticosteroids increases apoptosis in human rotator cuff tears.

Connect Tissue Res 2018 Aug 9:1-8. Epub 2018 Aug 9.

a Escuela de Medicina Veterinaria, Facultad de Ciencias de la Vida , Universidad Andres Bello , Santiago , Chile.

Purpose: The aim of this study is to evaluate in vivo the level of apoptosis in human rotator cuff tears and the relationship it might have with tendon degeneration.

Methods: Rotator cuff biopsies from 19 male and female patients, ages between 38 and 68 years, with and without previous corticosteroid infiltrations were collected via arthroscopy. Biopsies from seven patients with healthy rotator cuffs were used as a control group. Read More

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http://dx.doi.org/10.1080/03008207.2018.1501040DOI Listing
August 2018
3 Reads

Modeling and measuring extracellular matrix alterations in fibrosis: challenges and perspectives for antifibrotic drug discovery.

Connect Tissue Res 2019 Jan 3;60(1):62-70. Epub 2018 Aug 3.

a Fibrosis Translational Research and Development , Bristol-Myers Squibb , Pennington , NJ , USA.

An imbalance of extracellular matrix (ECM) deposition and turnover is a hallmark of fibrotic pathologies as opposed to normal repair response to injury across several organs. Antifibrotic approaches to date have targeted multiple mechanisms and pathways involved in inflammation, angiogenesis, injury, wound repair, ECM biosynthesis, assembly, crosslinking and degradation. Many of these approaches have been unsuccessful which may in part be due to suboptimal models and the lack of validated functional ECM end points relevant to fibrosis. Read More

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https://www.tandfonline.com/doi/full/10.1080/03008207.2018.1
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http://dx.doi.org/10.1080/03008207.2018.1500557DOI Listing
January 2019
4 Reads

Immunolocalization of enamel matrix protein-like proteins in the tooth enameloid of spotted gar, Lepisosteus oculatus, an actinopterygian bony fish.

Connect Tissue Res 2018 Jul 31. Epub 2018 Jul 31.

f Department of Oral Biology , Graduate School of Biomedical Sciences, Hiroshima University , 1-2-3 Kasumi, Hiroshima 734-8553 , Japan.

Enameloid is a well-mineralized tissue covering the tooth surface in fish and it corresponds to the outer-most layer of dentin. It was reported that both dental epithelial cells and odontoblasts are involved in the formation of enameloid. Nevertheless, the localization and timing of secretion of ectodermal enamel matrix proteins (EMPs) in enameloid are unclear. Read More

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http://dx.doi.org/10.1080/03008207.2018.1506446DOI Listing
July 2018
1 Read

3D-printed poly(lactic acid) scaffolds for trabecular bone repair and regeneration: scaffold and native bone characterization.

Connect Tissue Res 2018 Jul 30:1-9. Epub 2018 Jul 30.

a Department of Biomedical Engineering , TOBB University of Economics and Technology , Ankara , Turkey.

Purpose: Study objectives were set to (i) fabricate 3D-printed scaffolds/grafts with varying pore sizes, (ii) characterize surface and mechanical properties of scaffolds, (iii) characterize biomechanical properties of bovine trabecular bone, and (iv) evaluate attachment and proliferation of human bone marrow mesenchymal stem cells on 3D-printed scaffolds.

Materials And Methods: Poly(lactic acid) scaffolds were fabricated using 3D-printing technology, and characterized in terms of their surface as well as compressive mechanical properties. Trabecular bone specimens were obtained from bovine and characterized biomechanically under compression. Read More

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http://dx.doi.org/10.1080/03008207.2018.1499732DOI Listing
July 2018
2 Reads

Oncostatin M and its role in fibrosis.

Connect Tissue Res 2019 Jan 30;60(1):40-49. Epub 2018 Jul 30.

a School of Medicine, Section of Rheumatology , Boston University , Boston , MA , USA.

Oncostain M, a member of the IL-6 family of cytokines, is produced by immune cells in response to infections and tissue injury. OSM has a broad, often context-dependent effect on various cellular processes including differentiation, hematopoiesis, cell proliferation, and cell survival. OSM signaling is initiated by binding to type I (LIFRβ/gp130) or type II (OSMRβ/gp130) receptor complexes and involves activation of Janus kinase/signal transducer and activator of transcription, mitogen-activated protein kinase, and phosphatidylinositol-3-kinase. Read More

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http://dx.doi.org/10.1080/03008207.2018.1500558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6349219PMC
January 2019
2 Reads

Roles of large aggregating proteoglycans in human intervertebral disc degeneration.

Connect Tissue Res 2018 Aug 9:1-10. Epub 2018 Aug 9.

b School of Pharmaceutical Science , Jining Medical University , Rizhao , China.

Degeneration of the intervertebral discs, a natural progression of the aging process, is strongly implicated as a cause of low back pain. Aggrecan is the major structural proteoglycan in the extracellular matrix of the intervertebral disc. It is large, possessing numerous glycosaminoglycan chains and the ability to form aggregates in association with hyaluronan. Read More

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http://dx.doi.org/10.1080/03008207.2018.1499731DOI Listing
August 2018
2 Reads

Immunolocalization of transforming growth factor-beta1, connective tissue growth factor, phosphorylated-SMAD2/3, and phosphorylated-ERK1/2 during mouse incisor development.

Authors:
Shubo Li Yihuai Pan

Connect Tissue Res 2018 Oct 22:1-9. Epub 2018 Oct 22.

a The Institute of Stomatology, School and Hospital of Stomatology , Wenzhou Medical University , Wenzhou , Zhejiang Province , People's Republic of China.

Background/aims: Connective tissue growth factor (CTGF) is a downstream mediator of transforming growth factor-beta 1 (TGF-β) and TGF-β-induced CTGF expression is regulated through SMAD and mitogen-activated protein kinase (MAPK) signaling pathways. However, little is known about the localization of CTGF and TGF-β signaling cascades during incisor development. Therefore, we aimed to investigate the distribution pattern of TGF-β, CTGF, phosphorylated-SMAD2/3 (p-SMAD2/3), and phosphorylated-ERK1/2 (p-ERK1/2) in the developing mouse incisors. Read More

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https://www.tandfonline.com/doi/full/10.1080/03008207.2018.1
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http://dx.doi.org/10.1080/03008207.2018.1499730DOI Listing
October 2018
5 Reads

Short time insulin treatment post burn improves elastic-collagen rearrangement and reepithelization.

Connect Tissue Res 2018 Jul 11:1-10. Epub 2018 Jul 11.

a Department of Biosciences , Federal University of São Paulo, UNIFESP , SP , Brazil.

Extensive burn may cause acute resistance to insulin, which accentuates hypermetabolism, impairs glucose metabolism, immune dysfunction and risks of sepsis. To minimize these effects, insulin is used as a treatment. The purpose was to analyze the collagen-elastic arrangement effects of insulin on the burned skin. Read More

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http://dx.doi.org/10.1080/03008207.2018.1484916DOI Listing
July 2018
11 Reads

Biomechanical properties of the repaired and non-repaired rat supraspinatus tendon in the acute postoperative period.

Connect Tissue Res 2018 Jul 10:1-11. Epub 2018 Jul 10.

c Department of Orthopaedic Surgery & Medsport , University of Michigan , Ann Arbor , MI , USA.

Purpose: The rat rotator cuff (RC) model is used to study RC pathology and potential treatment; however, native scar-mediated healing allows the rat RC to recover at 4-6 weeks but little is known about acute healing. This study characterized the properties of the repaired and non-repaired rat RC following surgical detachment.

Materials And Methods: Forty-eight rats underwent surgical RC detachment and received surgical repair (Repair) or left unrepaired (Defect) to either 12 or 19 days. Read More

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http://dx.doi.org/10.1080/03008207.2018.1488970DOI Listing
July 2018
2 Reads
1.610 Impact Factor

Uncorrelated healing response of tendon and ear injuries in MRL highlight a role for the local tendon environment in driving scarless healing.

Connect Tissue Res 2018 Sep 21;59(5):472-482. Epub 2018 Jun 21.

a Nancy E. and Peter C. Meinig School of Biomedical Engineering , Cornell University , Ithaca , NY , USA.

Purpose: Tendon tears are common injuries that heal with scar formation. Interestingly, MRL/MpJ mice heal without scar in several tissues, including tendon. Most hypotheses regarding scarless healing implicate the systemic environment. Read More

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http://dx.doi.org/10.1080/03008207.2018.1485665DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175637PMC
September 2018
2 Reads

Effects of different oxygen concentrations on the proliferation, survival, migration, and osteogenic differentiation of MC3T3-E1 cells.

Connect Tissue Res 2018 Jun 19. Epub 2018 Jun 19.

a Department of Spine Orthopedics , Zhujiang Hospital, Southern Medical University , Guangzhou , China.

In physiological and pathological environments, the concentration of oxygen around osteoblasts varies widely. No studies have systematically evaluated the effects of different oxygen concentrations on the proliferation, survival, migration, and osteogenic differentiation of osteoblasts. In this study, we cultured the osteoblast precursor cell line MC3T3-E1 in small individual chambers with oxygen concentrations of 1%, 3%, 6%, 9%, and 21%. Read More

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http://dx.doi.org/10.1080/03008207.2018.1487413DOI Listing
June 2018
3 Reads

Partial-width injuries of the rat rotator cuff heal with fibrosis.

Connect Tissue Res 2018 Sep 2;59(5):437-446. Epub 2018 Jul 2.

b Department of Biomedical Engineering , University of Delaware , Newark , DE , USA.

Purpose: Identify the healing outcomes following a partial-width, full-thickness injury to the rotator cuff tendon-bone attachment and establish if the adult attachment can regenerate the morphology of the healthy attachment.

Hypothesis: We hypothesized that a partial-width injury to the attachment would heal via fibrosis and bone remodeling, resulting in increased cellularity and extra-cellular matrix deposition, reduced bone volume (BV), osteoclast presence, and decreased collagen organization compared to shams.

Materials And Methods: A partial-width injury was made using a biopsy punch at the center one-third of the rat infraspinatus attachment. Read More

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http://dx.doi.org/10.1080/03008207.2018.1485666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324170PMC
September 2018
4 Reads

Multiscale mechanical effects of native collagen cross-linking in tendon.

Connect Tissue Res 2018 Sep 6;59(5):410-422. Epub 2018 Jun 6.

a Department of Biomedical Engineering , Washington University in St. Louis , St. Louis , USA.

The hierarchical structure of tendon allows for attenuation of mechanical strain down decreasing length scales. While reorganization of collagen fibers accounts for microscale strain attenuation, cross-linking between collagen molecules contributes to deformation mechanisms at the fibrillar and molecular scales. Divalent and trivalent enzymatic cross-links form during the development of collagen fibrils through the enzymatic activity of lysyl oxidase (LOX). Read More

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http://dx.doi.org/10.1080/03008207.2018.1449837DOI Listing
September 2018
2 Reads

Histopathological, immunohistochemical, and biomechanical effects of splenectomy on Achilles tendon healing in rats.

Connect Tissue Res 2018 Jun 19:1-9. Epub 2018 Jun 19.

d Center for Regenerative Medicine and Stem Cell Research & Manufacturing (LivMedCell) , Istanbul , Turkey.

Purpose: This study aimed to assess Achilles tendon repair in rats following splenectomy to simulate patients with musculoskeletal system injury who had splenectomy after spleen injury, a situation often seen in orthopedics and traumatology practice.

Materials And Methods: The study included 32 male Sprague-Dawley rats (10 months old; average weight, 394.5 ± 28. Read More

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http://dx.doi.org/10.1080/03008207.2018.1483361DOI Listing
June 2018
18 Reads

Common gene variants interactions related to uric acid transport are associated with knee osteoarthritis susceptibility.

Connect Tissue Res 2018 Jul 11:1-11. Epub 2018 Jul 11.

a Musculoskeletal and Rheumatic Diseases Division , Synovial Fluid Laboratory, National Institute of Rehabilitation "Luis Guillermo Ibarra Ibarra" , Mexico City , Mexico.

Background: The presence of genetic variants in uric acid (UA) transporters can be associated with hyperuricemia, and therefore with an increased risk of monosodium urate (MSU) crystal precipitation. The inflammatory process triggered by these crystals leads to cartilage damage, which, in turn, could promote knee osteoarthritis (KOA).

Objective: To determine whether genetic polymorphisms of the UA transporters and their interactions are associated with KOA. Read More

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http://dx.doi.org/10.1080/03008207.2018.1483359DOI Listing
July 2018
6 Reads

MicroRNA-155 suppresses the catabolic effect induced by TNF-α and IL-1β by targeting C/EBPβ in rat nucleus pulposus cells.

Connect Tissue Res 2018 Oct 4:1-13. Epub 2018 Oct 4.

b Department of Spine Surgery , Sun Yat-sen Memorial Hospital of Sun Yat-sen University , Guangzhou , China.

Aim: miR-155 is a pro-inflammatory or anti-inflammatory factor depending on the cell type in which it is expressed. miR-155 controls apoptosis and matrix degradation in nucleus pulposus (NP) cells in vitro. The aim of this study is to explore the effect of miR-155 in vivo and further investigate the mechanism of miR-155 in vitro. Read More

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http://dx.doi.org/10.1080/03008207.2018.1483356DOI Listing
October 2018
20 Reads

CKIP-1 suppresses odontoblastic differentiation of dental pulp stem cells via BMP2 pathway and can interact with NRP1.

Connect Tissue Res 2018 Aug 14:1-10. Epub 2018 Aug 14.

d Department of Stomatology , Jiangsu Taizhou People's Hospital , Taizhou , Jiangsu , China.

Aim: Casein kinase 2 interacting protein-1 (CKIP-1) is a recently discovered intracellular regulator of bone formation, muscle cell differentiation, and tumor cell proliferation. Our study aims to identify the inhibition of BMP2-Smad1/5 signaling by CKIP-1 in odontoblastic differentiation of human dental pulp stem cells (DPSCs).

Materials And Methods: DPSCs infected CKIP-1 siRNA or transfected CKIP-1 full-length plasmid were cultured in odontoblastic differentiation medium or added noggin (200 ng/mL) for 21 days. Read More

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http://dx.doi.org/10.1080/03008207.2018.1483355DOI Listing
August 2018
6 Reads

Optimizing adipose tissue extract isolation with stirred suspension culture.

Connect Tissue Res 2018 Jun 25:1-11. Epub 2018 Jun 25.

a State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Oral Regenerative Medicine & Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology , Sichuan University , Chengdu , China.

Objectives: Adherent culture which is used to collect adipose tissue extract (ATE) previously brings the problem of inhomogeneity and non-repeatability. Here we aim to extract ATE with stirred suspension culture to speed up the extraction process, stabilize the yield, and improve consistent potency metrics of ATE.

Materials And Methods: ATE was collected with adherent culture (ATE-A) and stirred suspension culture (ATE-S) separately. Read More

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https://www.tandfonline.com/doi/full/10.1080/03008207.2018.1
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http://dx.doi.org/10.1080/03008207.2018.1483357DOI Listing
June 2018
16 Reads

Isoquercitrin promotes the osteogenic differentiation of osteoblasts and BMSCs via the RUNX2 or BMP pathway.

Connect Tissue Res 2018 Jun 18:1-11. Epub 2018 Jun 18.

a Zhejiang Key Laboratory of Pathophysiology , Medical School of Ningbo University , Ningbo , People's Republic of China.

Aim: Isoquercitrin is widely present in fruits, vegetables and medicinal herbs. As a natural phytoestrogen, isoquercitrin has been considered a possible osteoporosis prevention option to avoid the risk of hormone therapy.

Materials And Methods: The cell proliferation of osteoblasts and bone mesenchymal stem cells (BMSCs) was examined by cell counting kit-8 (CCK-8). Read More

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http://dx.doi.org/10.1080/03008207.2018.1483358DOI Listing
June 2018
18 Reads
1.610 Impact Factor

Regulation of osteogenesis by long noncoding RNAs: An epigenetic mechanism contributing to bone formation.

Connect Tissue Res 2018 Dec;59(sup1):35-41

a Department of Biochemistry , Larner College of Medicine at the University of Vermont , Burlington , Vermont , USA.

Long noncoding RNAs (lncRNAs) have recently emerged as novel regulators of lineage commitment, differentiation, development, viability, and disease progression. Few studies have examined their role in osteogenesis; however, given their critical and wide-ranging roles in other tissues, lncRNAs are most likely vital regulators of osteogenesis. In this study, we extensively characterized lncRNA expression in mesenchymal cells during commitment and differentiation to the osteoblast lineage using a whole transcriptome sequencing approach (RNA-Seq). Read More

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http://dx.doi.org/10.1080/03008207.2017.1412432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5965257PMC
December 2018
7 Reads

Late stages of mineralization and their signature on the bone mineral density distribution.

Connect Tissue Res 2018 Dec;59(sup1):74-80

c Department of Biomaterials , Max Planck Institute of Colloids and Interfaces , Potsdam , Germany.

Purpose: Experimental measurements of bone mineral density distributions (BMDDs) enable a determination of secondary mineralization kinetics in bone, but the maximum degree of mineralization and how this maximum is approached remain uncertain. We thus test computationally different hypotheses on late stages of bone mineralization by simulating BMDDs in low-turnover conditions.

Materials And Methods: An established computational model of the BMDD that accounts for mineralization and remodeling processes was extended to limit mineralization to various maximum calcium capacities of bone. Read More

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http://dx.doi.org/10.1080/03008207.2018.1424149DOI Listing
December 2018
3 Reads

Crosstalk between neuropeptides SP and CGRP in regulation of BMP2-induced bone differentiation.

Connect Tissue Res 2018 Dec;59(sup1):81-90

a Department of Biological Sciences , Carnegie Mellon University , Pittsburgh , PA , USA.

Aim Of The Study: The peripheral nervous system is involved in regulation of bone metabolism via sensory and sympathetic innervation. Substance P (SP) and calcitonin gene-related peptide (CGRP) are two sensory neuropeptides that have been associated with regulation of osteogenic differentiation. However, the interaction between SP and CGRP both with each other and the bone morphogenetic protein 2 (BMP2) in regulation of osteogenic differentiation has not been studied. Read More

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http://dx.doi.org/10.1080/03008207.2017.1408604DOI Listing
December 2018
2 Reads

Novel insights into renal mineralization and stone formation through advanced imaging modalities.

Connect Tissue Res 2018 Dec;59(sup1):102-110

a Department of Urology , University of California San Francisco , San Francisco , California , USA.

Purpose/Aim: The most common kidney stone composed of calcium oxalate forms on interstitial calcium phosphate mineral known as a Randall's plaque (RP). Due to limited information about events leading to the initial deposition of nanometer size interstitial calcium phosphate pre-clusters, there continues to be a debate on the initial site of calcium phosphate deposition and factors leading to stone formation.

Materials And Methods: High-resolution X-ray micro-computed tomography (CT), and light and electron microscopy techniques were used to characterize human renal pyramids and five representative kidney stones with identifiable stems. Read More

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http://dx.doi.org/10.1080/03008207.2017.1409219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120852PMC
December 2018
7 Reads

Impaired mineral quality in dentin in X-linked hypophosphatemia.

Connect Tissue Res 2018 Dec;59(sup1):91-96

a Orofacial Pathologies, Imaging and Biotherapies Laboratory EA2496, Dental School , Paris Descartes University , Paris , France.

X-linked hypophosphatemia (XLH) is a skeletal disorder arising from mutations in the PHEX gene, transmitted in most cases as an X-linked dominant trait. PHEX deficiency leads to renal phosphate wasting and hypophosphatemia, as well as impaired mineralization of bone and dentin, resulting in severe skeletal and dental complications. Dentin mineralization defects appear as characteristic, large interglobular spaces resulting from the lack of fusion of calculospherites in the circumpulpal region during the mineralization process. Read More

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http://dx.doi.org/10.1080/03008207.2017.1417989DOI Listing
December 2018
4 Reads

The unique biomineralization transcriptome and proteome of Lytechinus variegatus teeth.

Connect Tissue Res 2018 Dec;59(sup1):20-29

a Feinberg School of Medicine, Department of Cell and Molecular Biology , Northwestern University , Chicago , IL , USA.

Background: Matrix-regulated biomineralization involves the specific nucleation and growth of mineral phases within or upon preformed structured organic matrices. We hypothesized that there might be a general mechanism whereby anionic, phosphorylated mineral ion-binding proteins assist in specifically locating the mineral ions with respect to the mineralizing structural organic matrix. Here we extended these studies to invertebrate mineralization in Lytechinus variegatus (Lv) teeth. Read More

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http://dx.doi.org/10.1080/03008207.2017.1408605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052879PMC
December 2018
6 Reads

Physicochemical analysis of human pulpal mineralization secondary to FAM20A mutations.

Connect Tissue Res 2018 Dec;59(sup1):46-51

a Laboratoire de Physiopathologie Orale Moléculaire, Centre de Recherche des Cordeliers, UMRS 1138 Institut National de la Santé et de la Recherche Médicale , Universités Paris-Descartes , Pierre-et-Marie-Curie, Paris-Diderot, Paris , France.

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http://dx.doi.org/10.1080/03008207.2018.1435644DOI Listing
December 2018
5 Reads