208,188 results match your criteria Complementary Therapies in Medicine [Journal]
Sante Publique 2018 Sep;S2(HS2):157-163
The purpose of this article is to analyse healthcare pathways for precarious people, based on the results of a field study conducted in 2011 and 2012 in the north of France and Burgundy. About 40 semi-directed interviews were conducted in order to collect healthcare pathways narratives revolving around various health events during the subject's life, and putting them in perspective with individual, familial stories as well as their environment (social environment, healthcare organization, etc.). Read More
Sante Publique 2018 Sep;S2(HS2):145-156
Introduction: Health literacy refers to the competences and resources required by individuals to meet the complex demands of health in modern society. This paper describes and analyses the health literacy profiles of type 2 diabetic patients included in a 2-year long self-management education programme.
Methods: Nested in the ERMIES randomized controlled trial conducted in Reunion island, the ERMIES Ethnosocio study explored health literacy by means of two complementary approaches: description of health literacy profiles via the French version of the multidimensional ?Health Literacy Questionnaire?, and a socio-anthropological perspective based on 40 semi-structured interviews carried out in 2012 and then in 2015. Read More
Methods Mol Biol 2019 ;1883:217-233
School of Informatics, University of Edinburgh, Edinburgh, UK.
Inference of gene regulatory networks (GRNs) from time series data is a well-established field in computational systems biology. Most approaches can be broadly divided in two families: model-based and model-free methods. These two families are highly complementary: model-based methods seek to identify a formal mathematical model of the system. Read More
Inflammation 2018 Dec 14. Epub 2018 Dec 14.
Postgraduate Program of Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
Ziprasidone (ZIP) is an effective antipsychotic with low side effects than other second-generation antipsychotics. Despite this, there are reports of adverse events and previous studies associating the use of ZIP the inflammatory response. It is possible to infer that bioactive molecules present in some foods could attenuate peripheral inflammatory and oxidative stress potentially triggered ZIP. Read More
Theor Appl Genet 2018 Dec 13. Epub 2018 Dec 13.
Institute for Genomic Diversity, Institute of Biotechnology, Cornell University, 175 Biotechnology Building, Ithaca, NY, 14853, USA.
In the past, plant breeding has undergone three major transformations and is currently transitioning to a new technological phase, Breeding 4. This phase is characterized by the development of methods for biological design of plant varieties, including transformation and gene editing techniques directed toward causal loci. The application of such technologies will require to reliably estimate the effect of loci in plant genomes by avoiding the situation where the number of loci assayed (p) surpasses the number of plant genotypes (n). Read More
Health Serv Res Manag Epidemiol 2018 Jan-Dec;5:2333392818795795. Epub 2018 Dec 3.
AP-OSI Research Unit, Alto Deba Integrated Healthcare Organisation, Mondragón, Spain.
Introduction: An integrated health and social care program for patients with heart failure (HF) was implemented at the Friuli-Venezia Giulia deployment site as part of the SmartCare European project. The objective of this study was to validate 2 different decision modeling techniques used to perform the economic evaluation.
Methods: Data were collected during the SmartCare project which enrolled 108 patients with HF and followed for more than 6 months. Read More
Mol Clin Oncol 2018 Dec 8;9(6):622-628. Epub 2018 Oct 8.
Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
Metastatic cystic lesions may be considered as target lesions according to the Response Evaluation Criteria in Solid Tumors (RECIST) 1.1. However, cystic lesions are considered as non-measurable according to RECIST 1. Read More
Oncol Lett 2018 Dec 27;16(6):7074-7081. Epub 2018 Sep 27.
Department of Geriatrics, Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Alterations of mitochondrial DNA (mtDNA) have been identified in several types of solid tumor. However, to the best of our knowledge, the clinical significance of plasma mtDNA content in lung cancer remains unknown. Thus, the current study explored the diagnostic and prognostic value of plasma mtDNA quantification in patients with lung cancer. Read More
Front Neurorobot 2018 28;12:78. Epub 2018 Nov 28.
Knowledge Technology, Department of Informatics, Universität Hamburg, Hamburg, Germany.
Artificial autonomous agents and robots interacting in complex environments are required to continually acquire and fine-tune knowledge over sustained periods of time. The ability to learn from continuous streams of information is referred to as and represents a long-standing challenge for neural network models due to in which novel sensory experience interferes with existing representations and leads to abrupt decreases in the performance on previously acquired knowledge. Computational models of lifelong learning typically alleviate catastrophic forgetting in experimental scenarios with given datasets of static images and limited complexity, thereby differing significantly from the conditions artificial agents are exposed to. Read More
J Appl Crystallogr 2018 Dec 9;51(Pt 6):1623-1632. Epub 2018 Nov 9.
Department of Drug Design and Pharmacology, University of Copenhagen, Denmark.
Coupling of size-exclusion chromatography with biological solution small-angle X-ray scattering (SEC-SAXS) on dedicated synchrotron beamlines enables structural analysis of challenging samples such as labile proteins and low-affinity complexes. For this reason, the approach has gained increased popularity during the past decade. Transportation of perishable samples to synchrotrons might, however, compromise the experiments, and the limited availability of synchrotron beamtime renders iterative sample optimization tedious and lengthy. Read More
Emerg Microbes Infect 2018 Dec 13;7(1):212. Epub 2018 Dec 13.
Division of Chemical & System Biology, Department of Integrative Biomedical Science, University of Cape Town, Cape Town, South Africa.
Mycobacterium manages to evade the host cell immune system, partially owing to its ability to survive redox stress after macrophage engulfment. Exposure to redox stress has been linked to later replication, persistence, and latent infection. In this work, mass spectrometry was used to elucidate the cell-wide changes that occur in response to sublethal doses of hydrogen peroxide and nitric oxide over time, with Mycobacterium smegmatis being used as a model organism. Read More
Sci Rep 2018 Dec 13;8(1):17827. Epub 2018 Dec 13.
Center for Cognitive Neuroscience, Duke University, Durham, NC, USA.
Working memory (WM) is assumed to consist of a process that sustains memory representations in an active state (maintenance) and a process that operates on these activated representations (manipulation). We examined evidence for two distinct, concurrent cognitive functions supporting maintenance and manipulation abilities by testing brain activity as participants performed a WM alphabetization task. Maintenance was investigated by varying the number of letters held in WM and manipulation by varying the number of moves required to sort the list alphabetically. Read More
Eur Respir J 2018 Dec 13. Epub 2018 Dec 13.
Royal Papworth Hospital, Cambridge, UK.
Chronic thromboembolic pulmonary hypertension (CTEPH) is a complication of pulmonary embolism and a major cause of chronic PH leading to right heart failure and death. Lung ventilation/perfusion scintigraphy is the screening test of choice; a normal scan rules out CTEPH. In the case of an abnormal perfusion scan, a high-quality pulmonary angiogram is necessary to confirm and define the pulmonary vascular involvement and prior to making a treatment decision. Read More
J Biol Chem 2018 Dec 13. Epub 2018 Dec 13.
Systems Medicine, University of Dundee, United Kingdom.
GABAA receptors (GABAARs) are pentameric ligand-gated ion channels that mediate synaptic inhibition throughout the central nervous system. The α1β2γ2 receptor is the major subtype in the brain; GABA binds at the β2(+)α1(-) interface. The structure of the homomeric β3 GABAAR, which is not activated by GABA, has been solved. Read More
Science 2018 Dec 13;362(6420). Epub 2018 Dec 13.
Department of Neuroscience and Kavli Institute for Neuroscience, Yale School of Medicine, New Haven, CT, USA.
Human nervous system development is an intricate and protracted process that requires precise spatiotemporal transcriptional regulation. We generated tissue-level and single-cell transcriptomic data from up to 16 brain regions covering prenatal and postnatal rhesus macaque development. Integrative analysis with complementary human data revealed that global intraspecies (ontogenetic) and interspecies (phylogenetic) regional transcriptomic differences exhibit concerted cup-shaped patterns, with a late fetal-to-infancy (perinatal) convergence. Read More
Schizophr Res 2018 Dec 10. Epub 2018 Dec 10.
Department of Psychiatry and Psychotherapy, University Medical Center Hamburg Eppendorf, Hamburg, Germany.
Introduction: The relationship between childhood trauma and the risk of psychosis is well established. However, the mechanisms of the relationship are still unknown. We investigated whether two factors involved in the risk of psychosis - self-disturbances and aberrant salience - mediate the relationship between childhood trauma and psychotic-like experiences in the general population. Read More
Presse Med 2018 Dec 10. Epub 2018 Dec 10.
Hôpital Pitié-Salpêtrière (AP-HP), ACTION Study Group, Sorbonne Université, Université Paris 06 (UPMC), Inserm UMRS 1166, institut de cardiologie, Paris, France. Electronic address:
TAVI is currently recommended for severe symptomatic aortic stenosis in patients at prohibitive, high or intermediate surgical risk as estimated by surgical risk scores (STS score and EuroSCORE II). Comorbidity, technical feasibility of TAVI and concurrent pathology with need for complementary surgical correction are conditions to be considered to choose between open surgery and TAVI. The decision is often made by the "Heart Team". Read More
Acad Radiol 2018 Dec 11. Epub 2018 Dec 11.
Department of Physics, CNR ISC, UOS Roma Sapienza, Sapienza University of Rome, Rome, Italy.
Rationale And Objectives: To investigate the performance of diffusion kurtosis imaging (DKI) and diffusion tensor imaging (DTI) in discriminating benign tissue, low- and high-grade prostate adenocarcinoma (PCa).
Materials And Methods: Forty-eight patients with biopsy-proven PCa of different Gleason grade (GG), who provided written informed consent, were enrolled. All subjects underwent 3T DWI examinations by using b values 0, 500, 1000, 1500, 2000, and 2500 s/mm and six gradient directions. Read More
J Fr Ophtalmol 2018 Dec 10. Epub 2018 Dec 10.
Service d'ophtalmologie, Chu Treichville, Abidjan, Côte d'Ivoire.
Introduction: The treatment of primary open angle glaucoma (POAG) is preferably medical. However, when medical therapy fails, alternative or complementary treatments may be considered. In this regard, selective laser trabeculoplasty is a widely popular procedural treatment whose accepted benefits have been very little studied in African blacks. Read More
Int J Med Inform 2019 Jan 22;121:64-74. Epub 2018 Nov 22.
Department of Biomedical Informatics, University of Utah, Salt Lake City, UT 84108, USA.
Objective: There is a significant consumer demand for complementary and alternative medicine (CAM) therapies as possible alternatives to drugs in the treatment and prevention of chronic diseases. Expanding controlled vocabularies to include CAM treatment relations could help meet those needs by facilitating information retrieval from the published literature. The purpose of this study is to design and evaluate two methods to semi-automatically extract CAM treatment-related semantic predications (subject-predicate-object triplets) from the biomedical literature using the Semantic Medline database (SemMedDB). Read More
Nutr Hosp 2018 Apr 3;0(0):34-38. Epub 2018 Apr 3.
days of hospitalization, a greater number of complementary tests, the need for artificial nutritional support and / or drugs to treat complications, which is a very important economic burden. Most patients who are malnourished, or at risk, can benefit from oral nutritional support, which includes dietary modifications (fortification, extra snacks, etc.), prescription of a personalized diet by a registered dietitian and the use of oral nutritional supplements (ONS). Read More
Molecules 2018 Dec 12;23(12). Epub 2018 Dec 12.
Radiobiology Lab, The Greater Poland Cancer Centre, Garbary 15 Str., 61-866 Poznan, Poland.
Fucoidans have been reported to exert anticancer effects with simultaneous low toxicity against healthy tissue. That correlation was observed in several cancer models, however, it has never been investigated in head and neck cancer before. To magnify the efficacy of conventional therapy, the administration of agents like fucoidan could be beneficial. Read More
Medicina (Kaunas) 2018 Dec 12;54(6). Epub 2018 Dec 12.
Department of Biology-Chemistry, Advanced Environmental Research Laboratory, West University of Timisoara, Timisoara, 300086, Romania.
Erratic results have been published concerning the influence of the dietary supplement chitosan used as a complementary remedy to decrease the body weight of overweight and obese people. The published articles mention as secondary possible benefits of usage of chitosan the improvement of blood pressure and serum lipids status. We performed a meta-analysis evaluating body weight, body mass index, total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol, triglycerides, systolic and diastolic blood pressure among overweight and obese patients. Read More
Medicines (Basel) 2018 Dec 12;5(4). Epub 2018 Dec 12.
Biochemist, Gerberweg 7, 24211 Preetz, Germany.
This editorial describes a new hypothesis concerning the nature and possible mechanisms of the Nogier reflex or reflex auriculo-cardiac (RAC; also vascular autonomic signal VAS). A multimodal concept for future RAC research is proposed. Read More
Biosensors (Basel) 2018 Dec 10;8(4). Epub 2018 Dec 10.
Department of Aerospace & Mechanical Engineering, University of Arizona, Tucson, AZ 85721, USA.
Nuclear factor kappa B (NF-κB), regulating the expression of several genes that mediate the inflammatory responses and cell proliferation, is one of the therapeutic targets for chronic inflammatory disease and cancer. A novel molecular binding scheme for the detection of NF-κB was investigated for its affinity to Ig-κB DNA composed by dye and quencher fluorophores, and this specificity is confirmed by competing with the DNA sequence that is complementary to the Ig-κB DNA. We create a normalization equation to remove the negative effects from the various initial fluorophore concentrations and the background noise. Read More
Int J Mol Sci 2018 Dec 10;19(12). Epub 2018 Dec 10.
General Medicine and Metabolic Diseases, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milano 20122, Italy.
Nonalcoholic fatty liver disease (NAFLD) defines a wide pathological spectrum ranging from simple steatosis to nonalcoholic steatohepatitis (NASH) which may predispose to liver cirrhosis and hepatocellular carcinoma. It represents the leading cause of hepatic damage worldwide. Diagnosis of NASH still requires liver biopsy but due to the high prevalence of NAFLD, this procedure, which is invasive, is not practicable for mass screening. Read More
Molecules 2018 Dec 7;23(12). Epub 2018 Dec 7.
Department of Food Science, Yuanpei University of Medical Technology, No. 306, Yuanpei Street, Hsinchu 300, Taiwan.
Suppressing canonical NOD-like receptor protein 3 (NLRP3) inflammasome-mediated interleukin (IL)-1β secretion is a reliable strategy for the development of nutraceutical to prevent chronic inflammatory diseases. This study aimed to find out the functional group responsible for the inhibitory effects of cinnamaldehyde-related compounds on the canonical IL-1β secretion. To address this, the suppressing capacities of six cinnamaldehyde-related compounds were evaluated and compared by using the lipopolysaccharide (LPS)-primed and adenosine 5'-triphosphate (ATP)-activated macrophages. Read More
Int J Environ Res Public Health 2018 Dec 7;15(12). Epub 2018 Dec 7.
University of Waterloo. Ontario, Canada. 200 University Avenue West, Waterloo ON N2L 3G1, Canada.
For adolescent mothers in rural Eastern Uganda, nutrition and health may be compromised by many factors. Identifying individual and environmental needs and barriers at local levels is important to inform community-based interventions. This qualitative study used interviews based on constructs from social cognitive theory. Read More
Medicine (Baltimore) 2018 Dec;97(49):e13440
Dongfang Hospital of Beijing University of Chinese Medicine.
Background: Cancer-related fatigue (CRF) is a prevalent and debilitating symptom experienced by cancer survivors, one that severely compromises functional independence and quality of life. Clinical massage therapy (CMT), as an important part of complementary and alternative medicine, is widely employed among massage therapists, physical therapists, nurses, and physicians when managing CRF. Clinical research indicates that CMT produced relief of CRF. Read More
J Nutr 2018 Dec 12. Epub 2018 Dec 12.
Department of Food Technology, Safety, and Health, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
Background: The n-3 (ω-3) long-chain polyunsaturated fatty acid (LC-PUFA) docosahexaenoic acid (DHA) is essential for optimal brain development. There is a lack of evidence on the effect of postnatal n-3 LC-PUFA supplementation on child development in low-income countries.
Objective: We evaluated the efficacy of fish-oil supplementation through lactation or complementary food supplementation on the development of children aged 6-24 mo in rural Ethiopia. Read More
Biosens Bioelectron 2018 Nov 13;126:679-689. Epub 2018 Nov 13.
Department of Bioengineering, University of Texas at Dallas, 800 W. Campbell Road, Richardson, TX 75080, USA.
The tolerance, abuse, and potential exacerbation associated with classical chronic pain medications such as opioids creates a need for alternative therapeutics. Phenotypic screening provides a complementary approach to traditional target-based drug discovery. Profiling cellular phenotypes enables quantification of physiologically relevant traits central to a disease pathology without prior identification of a specific drug target. Read More
Spectrochim Acta A Mol Biomol Spectrosc 2018 Dec 4;211:221-226. Epub 2018 Dec 4.
Department of Physics, School of Technology and Applied Sciences, São Paulo State University (UNESP), Presidente Prudente, SP, Brazil. Electronic address:
Organic thin films are at the forefront of basic studies and applications in the field of physics, chemistry, biochemistry and materials science. For example, the intrinsic supramolecular arrangement, or simply the formation of aggregates may alter the optical and electrical properties, which would impact the potential applications of the material. Here, an attempt is made to correlate the molecular structures of two perylene derivatives, bis butylimido perylene (BuPTCD) and bis phenethylimido perylene (PhPTCD), with their film formation, in particular, the supramolecular arrangement and the photoluminescent properties. Read More
Rev Neurosci 2018 Dec 13. Epub 2018 Dec 13.
Neuroscience Unit, Laval University Medical Center (CHUL - CHU de Québec), 2705 Laurier Blvd, Quebec City, Quebec G1V 4G2, Canada.
Central pattern generators (CPGs) are generally defined as networks of neurons capable of enabling the production of central commands, specifically controlling stereotyped, rhythmic motor behaviors. Several CPGs localized in brainstem and spinal cord areas have been shown to underlie the expression of complex behaviors such as deglutition, mastication, respiration, defecation, micturition, ejaculation, and locomotion. Their pivotal roles have clearly been demonstrated although their organization and cellular properties remain incompletely characterized. Read More
J Womens Health (Larchmt) 2018 Dec 13. Epub 2018 Dec 13.
2 School of Public Health, University of Minnesota , Minneapolis, Minnesota.
Introduction: Meditation is a common type of complementary and alternative medicine (CAM), and the evidence for its usefulness for health promotion is growing. Women have higher rates of overall CAM use than men do, but little is known about gender differences in meditation practices, reasons for use, or perceived benefits.
Methods: Data from the 2012 National Health Interview Survey (NHIS) were used. Read More
Am J Physiol Gastrointest Liver Physiol 2018 Dec 13. Epub 2018 Dec 13.
Cattedra di Gastroenterologia, IRCCS Ospedale Maggiore, Italy.
Background: Jackhammer esophagus (JE) is a hypercontractile disorder, the pathogenesis of which is incompletely understood. Multiple rapid swallows (MRS) and rapid drink challenge (RDC) are complementary tests used during high resolution manometry (HRM) that evaluate inhibitory and excitatory neuromuscular function and latent obstruction respectively. Methods&aim. Read More
J Altern Complement Med 2018 Dec 13. Epub 2018 Dec 13.
Editor-in-Chief, The Journal of Alternative and Complementary Medicine.
J Altern Complement Med 2018 Dec 13. Epub 2018 Dec 13.
1 Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney , Ultimo, Australia .
ACS Nano 2018 Dec 13. Epub 2018 Dec 13.
Displaying the advantage of nanoparticles in cancer targeting and drug delivery, micelles have shown great potential in cancer therapy. The mechanism for micelle targeting to cancer without the need of ligand is due to the size advantage of micelles within the lower end of the nanometer scale that is the optimal size favoring Enhanced Permeability and Retention (EPR) effect while escaping trapping by macrophages. MicroRNAs (miRNAs) are ubiquitous and play critical roles in regulating gene expression, cell growth and cancer development. Read More
Anal Chem 2018 Dec 13. Epub 2018 Dec 13.
Developing a convenient method able to discriminate among different types of DNA nucleotides within a target sequence of the human genome is extremely challenging. We herein report an artificial ferrocene-base (Fe-base) that was synthesized and incorporated into different loci of a DNA strand. The Fe-base replacement on a nucleobase can interact with DNA bases and efficiently discriminate among A, T, G, C DNA bases of the complementary locus based on interacting electrochemical properties. Read More
J Gen Intern Med 2018 Dec 12. Epub 2018 Dec 12.
Yale-Griffin Prevention Research Center, Griffin Hospital, Yale University School of Public Health, Derby, CT, USA.
Background: Current treatment options for knee osteoarthritis have limited effectiveness and potentially adverse side effects. Massage may offer a safe and effective complement to the management of knee osteoarthritis.
Objective: Examine effects of whole-body massage on knee osteoarthritis, compared to active control (light-touch) and usual care. Read More
Methods Mol Biol 2019 ;1907:145-157
Department of Molecular Biology, UT Southwestern Medical Center, Dallas, TX, USA.
Transposon mutagenesis has emerged as a powerful methodology for functionally annotating cancer genomes. Although in vivo transposon-mediated forward genetic screens have proven to be valuable for cancer gene identification, they are also time consuming and resource intensive. To facilitate the rapid and cost-effective identification of genes that regulate tumor-promoting pathways, we developed a complementary ex vivo transposon mutagenesis approach wherein human or mouse cells growing in culture are mutagenized and screened for the acquisition of specific phenotypes in vitro or in vivo, such as growth factor independence or tumor-forming ability. Read More
Methods Mol Biol 2019 ;1907:137-144
Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA.
Since the advent of large-scale, detailed descriptive cancer genomics studies at the beginning of the century, such as The Cancer Genome Atlas (TCGA), labs around the world have been working to make this data useful. Data like these can be made more useful by comparison with complementary functional genomic data. One new example is the application of CRISPR/Cas9-based library screening for cancer-related traits in cell lines. Read More
Neurotherapeutics 2018 Dec 12. Epub 2018 Dec 12.
Department of Neurosurgery, Stanford University, 300 Pasteur Drive, Palo Alto, CA, 94304, USA.
Magnetic resonance imaging tractography permits in vivo visualization of white matter structures. Aside from its academic value, tractography has been proven particularly useful to neurosurgeons for preoperative planning. Preoperative tractography permits both qualitative and quantitative analyses of tumor effects upon surrounding white matter, allowing the surgeon to specifically tailor their operative approach. Read More
Oncol Rep 2018 Oct 9. Epub 2018 Oct 9.
Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan, R.O.C.
Ovarian cancer is the one of the most lethal gynecological cancer types. MicroRNAs (miRs) are noncoding RNAs that modulate the translation of their target mRNAs via binding to a complementary sequence in the target 3' untranslated region, and the dysregulation of certain miRs has been demonstrated to contribute to cancer progression. In this regard, the current study extended our previous work and used next‑generation sequencing data to search for upstream regulators of genetic alterations that are common in ovarian cancer, as well as the miRs that are involved in controlling the expression of these regulators. Read More
Analyst 2018 Dec 13. Epub 2018 Dec 13.
Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA.
In situ capillary microsampling with capillary electrophoresis (CE) electrospray ionization (ESI) mass spectrometry (MS) enabled the characterization of cationic metabolites in single cells in complex tissues and organisms. For deeper coverage of the metabolome and metabolic networks, analytical approaches are needed that provide complementary detection for anionic metabolites, ideally using the same instrumentation. Described here is one such approach that enables sequential cationic and anionic (dual) analysis of metabolites in the same identified cell in a live vertebrate embryo. Read More
Chem Sci 2018 Oct 7;9(37):7419-7425. Epub 2018 Aug 7.
Beijing Key Laboratory for Bioengineering and Sensing Technology , Research Center for Bioengineering and Sensing Technology , School of Chemistry and Biological Engineering , University of Science & Technology Beijing , Beijing 100083 , P. R. China . Email: ; Email:
Non-invasively imaging multiplex microRNAs (miRNAs) in living cells is pivotal to understanding their physiological functions and pathological development due to the key regulatory roles of miRNAs in gene expression. However, developing smart delivery systems with large gene loading capacity, biocompatibility and responsiveness remains a significant challenge. Herein, we successfully incorporated DNA-capped Au nanoparticles (NPs) and their complementary fluorescent DNA sequences into a porous 3D hydrogel network (AuDH), in which hairpin-locked DNAzyme strands and active metal ions were loaded (AuDH/M /H) for simultaneously imaging multiplex miRNAs in living cells. Read More
Front Microbiol 2018 27;9:2735. Epub 2018 Nov 27.
Biology Department, Viral Information Institute, San Diego State University, San Diego, CA, United States.
Rare Earth Elements (REEs) control methanol utilization in both methane- and methanol-utilizing microbes. It has been established that the addition of REEs leads to the transcriptional repression of MxaFI-MeDH [a two-subunit methanol dehydrogenase (MeDH), calcium-dependent] and the activation of XoxF-MeDH (a one-subunit MeDH, lanthanum-dependent). Both enzymes are pyrroquinoline quinone-dependent alcohol dehydrogenases and show significant homology; however, they display different kinetic properties and substrate specificities. Read More
Oncogene 2018 Dec 12. Epub 2018 Dec 12.
Department of Cancer Genetics & Genomics, Roswell Park Cancer Institute, Buffalo, NY, 14263, USA.
Multiple cancer signalling networks take part in regulatory crosstalks with the Hippo tumour suppressor pathway through the transcriptional cofactor Yes-associated protein (YAP). Nevertheless, how YAP is controlled by pathway crosstalks in tumourigenesis remains poorly understood. Here, we performed a targeted kinase inhibitor screen in human cancer cells to identify novel Hippo pathway regulators. Read More
Nat Protoc 2018 Dec 12. Epub 2018 Dec 12.
Department of Biology, Emory University, Atlanta, GA, USA.
Whole-genome bisulfite sequencing (WGBS) has been widely used to quantify cytosine DNA methylation frequency in an expanding array of cell and tissue types. Because of the denaturing conditions used, this method ultimately leads to the measurement of methylation frequencies at single cytosines. Hence, the methylation frequency of CpG dyads (two complementary CG dinucleotides) can be only indirectly inferred by overlaying the methylation frequency of two cytosines measured independently. Read More
J Virol 2018 Dec 12. Epub 2018 Dec 12.
School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom
Virus capsid proteins must perform a number of roles. These include the ability to self-assemble and to maintain stability under challenging environmental conditions, while retaining the conformational flexibility necessary to uncoat and deliver the viral genome into a host cell. Fulfilling these roles could place conflicting constraints on the innate abilities encoded within the protein sequences. Read More