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    2347 results match your criteria Complement Receptor Deficiency

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    Deficiency of Complement C3a and C5a Receptors Prevents Angiotensin II-Induced Hypertension via Regulatory T Cells.
    Circ Res 2018 Feb 5. Epub 2018 Feb 5.
    Shanghai Institute of Hypertension, Shanghai Jiao Tong University School of Medicine
    Inflammation and immunity play crucial roles in the development of hypertension. Complement activation-mediated innate immune response is involved in the regulation of hypertension and target organ damage. However, whether complement-mediated T cell functions could regulate blood pressure (BP) elevation in hypertension is still unclear. Read More

    In vitro evaluation of the biological activities of IgG in seven Chinese intravenous immunoglobulin preparations.
    J Pharm Biomed Anal 2018 Mar 16;151:317-323. Epub 2018 Jan 16.
    Institute of Blood Transfusion, Chinese Academy of Medical Sciences, 26 Huacai Road, Longtan Industry Park, Chenghua District, Chengdu, 610052, China. Electronic address:
    The IgG activities of antigen recognition, Fc-mediated complement activation and cellular Fcγ-receptors (FcγRs) binding are critical for intravenous immunoglobulin (IVIg) immunotherapy in a variety of immune deficiency diseases. Further, these activities could be influenced by different plasma sources and the IVIg manufacturing processes of different manufacturers. This study evaluated and compared the biological activities of IgG in 7 IVIg preparations produced by different Chinese manufacturers. Read More

    TNIP1 reduction sensitizes keratinocytes to post-receptor signalling following exposure to TLR agonists.
    Cell Signal 2018 Feb 5;45:81-92. Epub 2018 Feb 5.
    Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269-3092, USA; Stem Cell Institute, University of Connecticut, Storrs, CT 06269-3092, USA. Electronic address:
    Cell level inflammatory signalling is a combination of initiation at cell membrane receptors and modulation by cytoplasmic regulatory proteins. For keratinocytes, the predominant cell type in the epidermis, this would include toll-like receptors (TLR) and cytoplasmic proteins that propagate or dampen post-receptor signalling. We previously reported that increased levels of tumor necrosis factor α induced protein 3-interacting protein 1 (TNIP1) in HaCaT keratinocytes leads to decreased expression of stress response and inflammation-associated genes. Read More

    Splenic macrophages are required for protective innate immunity against West Nile virus.
    PLoS One 2018 6;13(2):e0191690. Epub 2018 Feb 6.
    Department of Immunology, University of Washington, Seattle, WA, United States of America.
    Although the spleen is a major site for West Nile virus (WNV) replication and spread, relatively little is known about which innate cells in the spleen replicate WNV, control viral dissemination, and/or prime innate and adaptive immune responses. Here we tested if splenic macrophages (MΦs) were necessary for control of WNV infection. We selectively depleted splenic MΦs, but not draining lymph node MΦs, by injecting mice intravenously with clodronate liposomes several days prior to infecting them with WNV. Read More

    Absence of myeloid Klf4 reduces prostate cancer growth with pro-atherosclerotic activation of tumor myeloid cells and infiltration of CD8 T cells.
    PLoS One 2018 11;13(1):e0191188. Epub 2018 Jan 11.
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
    The microenvironment of prostate cancer often includes abundant tumor-associated macrophages (TAMs), with their acquisition of an M2 phenotype correlating with local aggressiveness and metastasis. Tumor-derived M-CSF contributes to TAM M2 polarization, and M-CSF receptor inhibition slows prostate cancer growth in model systems. As additional cytokines can direct TAM M2 polarization, targeting downstream transcription factors could avoid resistance. Read More

    Effects of Salvia miltiorrhiza extract with supplemental liquefied calcium on osteoporosis in calcium-deficient ovariectomized mice.
    BMC Complement Altern Med 2017 Dec 20;17(1):545. Epub 2017 Dec 20.
    Department of Oriental Medicine and Biotechnology, College of Life Science, Kyung Hee University, Yongin-si, 17104, Republic of Korea.
    Background: Extracts from Salvia miltiorrhiza Bunge have been used in traditional Asian medicine to treat coronary heart disease, chronic renal failure, atherosclerosis, myocardial infraction, angina pectoris, myocardial ischemia, dysmenorrheal, neurasthenic insomnia, liver fibrosis and cirrhosis. The aim of the study was to investigate the anti-RANK signal effect of the combination of S.miltiorrhiza Bunge (SME) and liquefied calcium (LCa) supplement with ovariectomized (OVX-SML) mice, a osteoporosis animal model. Read More

    VISTA deficiency attenuates antibody-induced arthritis and alters macrophage gene expression in response to simulated immune complexes.
    Arthritis Res Ther 2017 Dec 8;19(1):270. Epub 2017 Dec 8.
    Department of Veterans Affairs, Research Service, White River Junction, VT, 05009, USA.
    Background: In addition to activated T cells, the immune checkpoint inhibitor "V domain-containing Ig suppressor of T-cell activation" (VISTA) is expressed by myeloid cell types, including macrophages and neutrophils. The importance of VISTA expression by myeloid cells to antibody-induced arthritis and its potential for relevance in human disease was evaluated.

    Methods: VISTA was immunolocalized in normal and arthritic human synovial tissue sections and synovial tissue lysates were subjected to western blot analysis. Read More

    Complement 5a Receptor deficiency does not influence adverse cardiac remodeling after pressure-overload in mice.
    Sci Rep 2017 Dec 6;7(1):17045. Epub 2017 Dec 6.
    Laboratory of Experimental Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
    Hypertension is one of the most common risk factors for the development heart failure in the general population. Inflammation plays a central role in this adverse remodeling and eventually to the development of heart failure. Circulating levels of Complement factor 5a (C5a) are increased in hypertensive patients and the C5a receptor is associated with the presence of cardiac fibrosis and inflammation in an experimental hypertension model. Read More

    Glycosylphosphatidylinositol (GPI) anchored protein deficiency serves as a reliable reporter of Pig-a gene Mutation: Support from an in vitro assay based on L5178Y/Tkcells and the CD90.2 antigen.
    Environ Mol Mutagen 2018 Jan 8;59(1):18-29. Epub 2017 Nov 8.
    Litron Laboratories, Rochester, New York.
    Lack of cell surface glycosylphosphatidylinositol (GPI)-anchored protein(s) has been used as a reporter of Pig-a gene mutation in several model systems. As an extension of this work, our laboratory initiated development of an in vitro mutation assay based on the flow cytometric assessment of CD90.2 expression on the cell surface of the mouse lymphoma cell line L5178Y/Tk. Read More

    Circadian clock cryptochrome proteins regulate autoimmunity.
    Proc Natl Acad Sci U S A 2017 Nov 6;114(47):12548-12553. Epub 2017 Nov 6.
    Department of Hematology and Oncology, Cedars-Sinai Medical Center, Los Angeles, CA 90048.
    The circadian system regulates numerous physiological processes including immune responses. Here, we show that mice deficient of the circadian clock genesand[double knockout (DKO)] develop an autoimmune phenotype including high serum IgG concentrations, serum antinuclear antibodies, and precipitation of IgG, IgM, and complement 3 in glomeruli and massive infiltration of leukocytes into the lungs and kidneys. Flow cytometry of lymphoid organs revealed decreased pre-B cell numbers and a higher percentage of mature recirculating B cells in the bone marrow, as well as increased numbers of B2 B cells in the peritoneal cavity ofDKO mice. Read More

    Autoantibodies against complement components in systemic lupus erythematosus - role in the pathogenesis and clinical manifestations.
    Lupus 2017 Dec 18;26(14):1550-1555. Epub 2017 May 18.
    2 Department of Chemistry, Biochemistry, Physiology and Pathophysiology, Sofia University "St. Kliment Ohridski", Bulgaria.
    Many complement structures and a number of additional factors, i.e. autoantibodies, receptors, hormones and cytokines, are implicated in the complex pathogenesis of systemic lupus erythematosus. Read More

    Electronegative Low-Density Lipoprotein L5 Induces Adipose Tissue Inflammation Associated With Metabolic Syndrome.
    J Clin Endocrinol Metab 2017 Dec;102(12):4615-4625
    Lipid Science and Aging Research Center, Kaohsiung Medical University, Taiwan.
    Context: Electronegative low-density lipoprotein (LDL) L5 is a naturally occurring, atherogenic entity found at elevated levels in the plasma of patients with metabolic syndrome (MetS) in the absence of elevated plasma LDL levels.

    Objective: To investigate the role of L5 in the mechanism of adipose tissue inflammation associated with MetS.

    Patients/setting: Plasma LDL isolated from patients with MetS (n = 29) and controls (n = 29) with similar plasma LDL levels was separated into five subfractions, L1 to L5, with increasing electronegativity. Read More

    Spred2-deficiecy Protects Mice from Polymicrobial Septic Peritonitis by Enhancing Inflammation and Bacterial Clearance.
    Sci Rep 2017 Oct 9;7(1):12833. Epub 2017 Oct 9.
    Department of Pathology and Experimental Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8558, Japan.
    Sepsis is an infection-induced systemic inflammatory syndrome and a major cause of death for critically ill patients. Here, we examined whether the absence of Sprouty-related EVH1-domain-containing protein 2 (Spred2), a negative regulator of the Ras/Raf/ERK/MAPK pathway, influences host defense against polymicrobial sepsis (PMS) induced by cecal ligation and puncture (CLP). Compared to wild-type mice, Spred2mice exhibited higher survival rates with increased level of leukocyte infiltration and local chemokine production and reduced plasma and peritoneal bacterial loads after CLP. Read More

    An ABC of the Warning Signs of Hereditary Angioedema.
    Int Arch Allergy Immunol 2017 27;174(1):1-6. Epub 2017 Sep 27.
    Faculdade de Medicina ABC, Santo Andre, Brazil.
    Hereditary angioedema (HAE) with C1 inhibitor deficiency is a genetic disorder that clinically manifests with attacks of angioedema in the subcutaneous and submucosal tissues, mainly in the extremities, abdomen, and upper airway. During attacks, vascular permeability is increased due to increased bradykinin (BK). This means that special therapies are needed for attacks that do not respond to traditional antiallergic therapies involving antihistamines, corticosteroids, and epinephrine. Read More

    Vitamin K-Dependent Protein S: Beyond the Protein C Pathway.
    Semin Thromb Hemost 2018 Mar 13;44(2):176-184. Epub 2017 Sep 13.
    Department of Translational Medicine, Lund University, Malmö, Sweden.
    Protein S is a vitamin K-dependent plasma glycoprotein circulating in plasma at a concentration of around 350 nM. Approximately 60% of protein S in human plasma is bound to the complement regulatory protein C4b-binding protein (C4BP) in a high-affinity, high-molecular-weight complex. Protein S in plasma has multiple anticoagulant properties and heterozygous protein S deficiency is associated with increased risk of venous thrombosis. Read More

    Complement C5a receptors C5L2 and C5aR in renal fibrosis.
    Am J Physiol Renal Physiol 2018 01 13;314(1):F35-F46. Epub 2017 Sep 13.
    Division of Gastroenterology, Saarland University Medical Center , Homburg , Germany.
    Complement factor C5a has two known receptors, C5aR, which mediates proinflammatory effects, and C5L2, a potential C5a decoy receptor. We previously identified C5a/C5aR signaling as a potent profibrotic pathway in the kidney. Here we tested for the first time the role of C5L2 in renal fibrosis. Read More

    CD18 deficiency improves liver injury in the MCD model of steatohepatitis.
    PLoS One 2017 5;12(9):e0183912. Epub 2017 Sep 5.
    Department of Medicine, University of California, San Francisco, San Francisco, California, United States of America.
    Neutrophils and macrophages are important constituents of the hepatic inflammatory infiltrate in non-alcoholic steatohepatitis. These innate immune cells express CD18, an adhesion molecule that facilitates leukocyte activation. In the context of fatty liver, activation of infiltrated leukocytes is believed to enhance hepatocellular injury. Read More

    Mac-1 deficiency induces respiratory failure by affecting type I alveolar epithelial cells.
    Genet Mol Res 2017 Aug 31;16(3). Epub 2017 Aug 31.
    Department of Nursing, People's Hospital of Laizhou City, Laizhou, , China.
    As a β2 integrin family member, Mac-1 plays an important role in the inflammatory response. Inflammation and lung injury are closely associated, but the involvement of Mac-1 in the occurrence and development of such pathologies remains poorly understood. We aimed to investigate the relationship between Mac-1 deficiency and respiratory failure in Mac-1 knockout {Mac-1} mice, using C57BL/6J mice as a control. Read More

    IgG is elevated in obese white adipose tissue but does not induce glucose intolerance via Fcγ-receptor or complement.
    Int J Obes (Lond) 2018 Feb 30;42(2):260-269. Epub 2017 Aug 30.
    Einthoven Laboratory for Experimental Vascular Medicine, Leiden, The Netherlands.
    Background/objectives: In obesity, B cells accumulate in white adipose tissue (WAT) and produce IgG, which may contribute to the development of glucose intolerance. IgG signals by binding to Fcγ receptors (FcγR) and by activating the complement system. The aim of our study was to investigate whether activation of FcγR and/or complement C3 mediates the development of high-fat diet-induced glucose intolerance. Read More

    IL-17A deficiency mitigates bleomycin-induced complement activation during lung fibrosis.
    FASEB J 2017 Dec 17;31(12):5543-5556. Epub 2017 Aug 17.
    Division of Pulmonary and Critical Care Medicine, University of Michigan, Ann Arbor, Michigan, USA;
    Interleukin 17A (IL-17A) and complement (C') activation have each been implicated in the pathogenesis of idiopathic pulmonary fibrosis (IPF). We have reported that IL-17A induces epithelial injuryTGF-β in murine bronchiolitis obliterans; that TGF-β and the C' cascade present signaling interactions in mediating epithelial injury; and that the blockade of C' receptors mitigates lung fibrosis. In the present study, we investigated the role of IL-17A in regulating C' in lung fibrosis. Read More

    The atypical receptor CCRL2 is required for CXCR2-dependent neutrophil recruitment and tissue damage.
    Blood 2017 09 25;130(10):1223-1234. Epub 2017 Jul 25.
    Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.
    CCRL2 is a 7-transmembrane domain receptor that shares structural and functional similarities with the family of atypical chemokine receptors (ACKRs). CCRL2 is upregulated by inflammatory signals and, unlike other ACKRs, it is not a chemoattractant-scavenging receptor, does not activate β-arrestins, and is widely expressed by many leukocyte subsets. Therefore, the biological role of CCRL2 in immunity is still unclear. Read More

    Disruption of the ATP/adenosine balance in CD39mice is associated with handling-induced seizures.
    Immunology 2017 12 25;152(4):589-601. Epub 2017 Aug 25.
    Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
    Seizures are due to excessive, synchronous neuronal firing in the brain and are characteristic of epilepsy, the fourth most prevalent neurological disease. We report handling-induced and spontaneous seizures in mice deficient for CD39, a cell-surface ATPase highly expressed on microglial cells. CD39mice with handling-induced seizures had normal input-output curves and paired-pulse ratio measured from hippocampal slices and lacked microgliosis, astrogliosis or overt cell loss in the hippocampus and cortex. Read More

    Anti-Neu5Gc and anti-non-Neu5Gc antibodies in healthy humans.
    PLoS One 2017 17;12(7):e0180768. Epub 2017 Jul 17.
    Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA, United States of America.
    Our group previously investigated the levels of anti-Gal and anti-nonGal IgM and IgG in a cohort of 75 healthy humans of various backgrounds, and found some significant differences related to factors such as age, gender, ABO blood group, diet, vaccination history, and geographic location during childhood. We have now expanded our cohort (n = 84) to investigate the levels of anti-Neu5Gc and anti-nonGal/nonNeu5Gc antibodies in healthy humans. Anti-nonGal and anti-nonGal/nonNeu5Gc human IgM and IgG binding to pRBCs and pAECs from GTKO/CD46 and GTKO/CD46/Neu5GcKO pigs were measured by flow cytometry. Read More

    Increased aggression and lack of maternal behavior in Dio3-deficient mice are associated with abnormalities in oxytocin and vasopressin systems.
    Genes Brain Behav 2018 Jan 4;17(1):23-35. Epub 2017 Aug 4.
    Maine Medical Center Research Institute, Center for Molecular Medicine, Scarborough, ME, USA.
    Thyroid hormones regulate many aspects of brain development and function, and alterations in the levels of thyroid hormone action lead to abnormal anxiety- and depression-like behaviors. A complement of factors in the brain function independently of circulating levels of hormone to strictly controlled local thyroid hormone signaling. A critical factor is the type 3 deiodinase (DIO3), which is located in neurons and protects the brain from excessive thyroid hormone. Read More

    Circulating dsDNA, endothelial injury, and complement activation in thrombotic microangiopathy and GVHD.
    Blood 2017 09 13;130(10):1259-1266. Epub 2017 Jul 13.
    Division of Bone Marrow Transplantation and Immune Deficiency and.
    Transplant-associated thrombotic microangiopathy (TA-TMA) is a common and poorly recognized complication of hematopoietic stem cell transplantation (HSCT) associated with excessive complement activation, likely triggered by endothelial injury. An important missing piece is the link between endothelial injury and complement activation. We hypothesized that neutrophil extracellular traps (NETs) mechanistically link endothelial damage with complement activation and subsequent TA-TMA. Read More

    Interferon Regulatory Factor 5 Controls Necrotic Core Formation in Atherosclerotic Lesions by Impairing Efferocytosis.
    Circulation 2017 Sep 11;136(12):1140-1154. Epub 2017 Jul 11.
    From Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, United Kingdom (A.N.S., A.E., J.E.C., C.K., M.S., I.P., P.G., T.K., D.S., M.E.G., S.N.S., I.A.U., C.M.); Department of Bioengineering, Imperial College London, United Kingdom (A.N.S., R.K.); Experimental Cardiovascular Research Unit, Clinical Research Centre, Clinical Sciences Malmö, Lund University, Sweden (A.E., I.G.); Department of Cardiology, Skåne University Hospital, Lund/Malmö, Sweden (A.E., I.G.); and School of Engineering and Materials Science, Queen Mary University of London, United Kingdom (R.K.).
    Background: Myeloid cells are central to atherosclerotic lesion development and vulnerable plaque formation. Impaired ability of arterial phagocytes to uptake apoptotic cells (efferocytosis) promotes lesion growth and establishment of a necrotic core. The transcription factor interferon regulatory factor (IRF)-5 is an important modulator of myeloid function and programming. Read More

    Interferon-β regulates dendritic cell activation and migration in experimental autoimmune encephalomyelitis.
    Immunology 2017 11 21;152(3):439-450. Epub 2017 Jul 21.
    Toronto General Research Institute, University Health Network, Toronto, ON, Canada.
    CD11cdendritic cells (DCs) exert a critical role as antigen-presenting cells in regulating pathogenic T cells in multiple sclerosis (MS). To determine whether the therapeutic benefit of interferon-β (IFN-β) treatment for MS is in part influenced by IFN regulation of DC function, we examined the immunophenotype of DCs derived from IFN-βand IFN-βmice using a myelin oligodendrocyte glycoprotein (MOG) peptide-induced mouse model of MS, experimental autoimmune encephalomyelitis (EAE). Our earlier work identified that IFN-βmice exhibit earlier onset and more rapid progression of neurological impairment compared with IFN-βmice. Read More

    MECHANISMS OF COIX SEED COMPOSITIONS IN THE TREATMENT OF SPLEEN DEFICIENCY AND WET DAMPNESS ZHENG.
    Afr J Tradit Complement Altern Med 2017 5;14(4):239-246. Epub 2017 Jun 5.
    College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China.
    Background: Coix seed has the functions of fortifying the spleen and inhibiting the dampness. However, it remains unclear which Coix seed compositions is responsible for these functions. Previous investigations have revealed that the main compositions of Coix seed are proteins, polysaccharides, oils and starches. Read More

    Measurement of Aortic Cell Fluid-Phase Pinocytosis in vivo by Flow Cytometry.
    J Vasc Res 2017 16;54(4):195-199. Epub 2017 Jun 16.
    Section of Experimental Atherosclerosis, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD, USA.
    Objective: Fluid-phase pinocytosis is a receptor-independent mechanism of endocytosis that occurs in all mammalian cells and may be a mechanism for the uptake of LDL by macrophages. As there are currently no methods for the measurement of fluid-phase pinocytosis by individual aortic cells in vivo, we sought to identify a suitable method.

    Methods: ApoE-/- mice were retro-orbitally injected with AngioSPARK fluorescent nanoparticles specifically designed to not interact with cells. Read More

    Glycolipids: Essential regulator of neuro-inflammation, metabolism and gliomagenesis.
    Biochim Biophys Acta 2017 10 7;1861(10):2479-2484. Epub 2017 Jun 7.
    Department of Biomedical Sciences, Chubu University College of Life and Health, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan; Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065, Japan.
    Gene knockout mice of glycosyltransferases have clearly showed roles of their products in the bodies, while there are examples where phenotype of knockout was much less severe than expected probably due to functional redundancy. The most striking novel finding obtained from ganglioside-deficient mice was that progressive inflammatory reaction took place, leading to neurodegeneration. In particular, dysfunction of complement-regulatory proteins due to deteriorated architecture of lipid rafts seemed to be essential mechanisms for the inflammation. Read More

    Complement C5a induces mesenchymal stem cell apoptosis during the progression of chronic diabetic complications.
    Diabetologia 2017 Sep 3;60(9):1822-1833. Epub 2017 Jun 3.
    First Department, State Key Laboratory of Trauma, Burn and Combined Injury, Daping Hospital and Research Institute of Surgery, Third Military Medical University, No. 10 Changjiang Branch Road, Daping Street, Yuzhong District, Chongqing, 400042, People's Republic of China.
    Aims/hypothesis: Regeneration and repair mediated by mesenchymal stem cells (MSCs) are key self-protection mechanisms against diabetic complications, a reflection of diabetes-related cell/tissue damage and dysfunction. MSC abnormalities have been reported during the progression of diabetic complications, but little is known about whether a deficiency in these cells plays a role in the pathogenesis of this disease. In addition to MSC resident sites, peripheral circulation is a major source of MSCs that participate in the regeneration and repair of damaged tissue. Read More

    gene disruption is associated with increased hematopoietic stem cells: implication in chronic hypoxia-induced pulmonary hypertension.
    Am J Physiol Heart Circ Physiol 2017 Aug 26;313(2):H293-H303. Epub 2017 May 26.
    Department of Pharmacology, and Translation Cardiovascular Institute, School of Medicine, New York Medical College, Valhalla, New York;
    We have recently demonstrated that disruption of the murine cytochrome-450 2c44 gene (exacerbates chronic hypoxia-induced pulmonary artery remodeling and hypertension in mice. Subsequently, we serendipitously found thatgene disruption also increases hematopoietic stem cell (HSC) numbers in bone marrow and blood. Therefore, the objective of the present study was to investigate whether CYP2C44-derived eicosanoids regulate HSC proliferation/cell growth and whether increased HSCs contribute to chronic hypoxia-induced remodeling of pulmonary arteries inknockout mice. Read More

    Necrotizing Ulcer After BCG Vaccination in a Girl With Leukocyte-adhesion Deficiency Type 1.
    J Pediatr Hematol Oncol 2018 Jan;40(1):63-66
    Department of Pediatrics, Dokkyo Medical University School of Medicine.
    Leukocyte-adhesion deficiency-1 is a recessively inherited disorder associated with recurrent bacterial infections, severe periodontitis, peripheral leukocytosis, and impaired wound healing. We diagnosed moderate-type leukocyte-adhesion deficiency-1 in a 7-year-old girl who developed a necrotizing ulcer after Bacillus Calmette-Guerin vaccination. The patient showed moderate expression of CD18 in neutrophils with a homozygous splice mutation with c. Read More

    The Upregulation of Integrin αβ(CD11d/CD18) on Inflammatory Macrophages Promotes Macrophage Retention in Vascular Lesions and Development of Atherosclerosis.
    J Immunol 2017 06 12;198(12):4855-4867. Epub 2017 May 12.
    Department of Biomedical Sciences, Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37604;
    Macrophage accumulation is a critical step during development of chronic inflammation, initiating progression of many devastating diseases. Leukocyte-specific integrin αβ(CD11d/CD18) is dramatically upregulated on macrophages at inflammatory sites. Previously we found that CD11d overexpression on cell surfaces inhibits in vitro cell migration due to excessive adhesion. Read More

    Recurrent respiratory tract infections (RRTI) in the elderly: A late onset mild immunodeficiency?
    Clin Immunol 2017 07 6;180:111-119. Epub 2017 May 6.
    Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
    Elderly with late-onset recurrent respiratory tract infections (RRTI) often have specific anti-polysaccharide antibody deficiency (SPAD). We hypothesized that late-onset RRTI is caused by mild immunodeficiencies, such as SPAD, that remain hidden through adult life. We analyzed seventeen elderly RRTI patients and matched controls. Read More

    Dietary supplementation with long-chain monounsaturated fatty acid isomers decreases atherosclerosis and alters lipoprotein proteomes in LDLrmice.
    Atherosclerosis 2017 Jul 25;262:31-38. Epub 2017 Apr 25.
    Lipoprotein Metabolism Section, Cardio-Pulmonary Branch, National Heart, Lung and Blood Institute (NHLBI), National Institutes of Health (NIH), Bethesda, MD 20892-1666, USA. Electronic address:
    Background And Aims: Concentrated fish oils, containing a mixture of long-chain monounsaturated fatty acids (LCMUFA) with aliphatic chains longer than 18 C atoms (i.e., C20:1 and C22:1), have been shown to attenuate atherosclerosis development in mouse models. Read More

    Peripheral complement interactions with amyloid β peptide: Erythrocyte clearance mechanisms.
    Alzheimers Dement 2017 Dec 2;13(12):1397-1409. Epub 2017 May 2.
    Center for Neuroscience, SRI International, Menlo Park, CA, USA. Electronic address:
    Introduction: Although amyloid β peptide (Aβ) is cleared from the brain to cerebrospinal fluid and the peripheral circulation, mechanisms for its removal from blood remain unresolved. Primates have uniquely evolved a highly effective peripheral clearance mechanism for pathogens, immune adherence, in which erythrocyte complement receptor 1 (CR1) plays a major role.

    Methods: Multidisciplinary methods were used to demonstrate immune adherence capture of Aβ by erythrocytes and its deficiency in Alzheimer's disease (AD). Read More

    Dysregulated CD46 shedding interferes with Th1-contraction in systemic lupus erythematosus.
    Eur J Immunol 2017 07 22;47(7):1200-1210. Epub 2017 May 22.
    Division of Genetics and Molecular Medicine, Department of Medical and Molecular Genetics, King's College London, Guy's Hospital, London, UK.
    IFN-γ-producing T helper 1 (Th1) cell responses mediate protection against infections but uncontrolled Th1 activity also contributes to a broad range of autoimmune diseases. Autocrine complement activation has recently emerged as key in the induction and contraction of human Th1 immunity: activation of the complement regulator CD46 and the C3aR expressed by CD4T cells via autocrine generated ligands C3b and C3a, respectively, are critical to IFN-γ production. Further, CD46-mediated signals also induce co-expression of immunosuppressive IL-10 in Th1 cells and transition into a (self)-regulating and contracting phase. Read More

    C1q-Dependent Dendritic Cell Cross-Presentation of In Vivo-Formed Antigen-Antibody Complexes.
    J Immunol 2017 06 21;198(11):4235-4243. Epub 2017 Apr 21.
    Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2300 RC Leiden, the Netherlands;
    Dendritic cells (DCs) are specialized in Ag engulfment via a wide variety of uptake receptors on their cell surface. In the present study we investigated Ag uptake and presentation of in vivo-formed Ag-Ab complexes by i.v. Read More

    P2X, P2X, and P2XReceptor Knock Out Mice Expose Differential Outcome of Sepsis Induced by α-Haemolysin Producing.
    Front Cell Infect Microbiol 2017 6;7:113. Epub 2017 Apr 6.
    Department of Biomedicine, Aarhus UniversityAarhus, Denmark.
    α-haemolysin (HlyA)-producingcommonly inflict severe urinary tract infections, including pyelonephritis, which comprises substantial risk for sepsis., the cytolytic effect of HlyA is mainly mediated by ATP release through the HlyA pore and subsequent P2X/P2Xreceptor activation. This amplification of the lytic process is not unique to HlyA but is observed by many other pore-forming proteins including complement-induced haemolysis. Read More

    HVC1 ameliorates hyperlipidemia and inflammation in LDLRmice.
    BMC Complement Altern Med 2017 Apr 20;17(1):222. Epub 2017 Apr 20.
    College of Korean Medicine, Sangji University, Wonju-si, Gangwon-do, 26339, Republic of Korea.
    Background: HVC1 consists of Coptidis Rhizoma (dried rhizome of Coptischinensis), Scutellariae Radix (root of Scutellariabaicalensis), Rhei Rhizoma (rhizome of Rheum officinale), and Pruni Cortex (cortex of Prunusyedoensis Matsum). Although the components are known to be effective in various conditions such as inflammation, hypertension, and hypercholesterolemia, there are no reports of the molecular mechanism of its hypolipidemic effects.

    Methods: We investigated the hypolipidemic effect of HVC1 in low-density lipoprotein receptor-deficient (LDLR) mice fed a high-cholesterol diet for 13 weeks. Read More

    Aquaporin-4 Mediates the Suppressive Effect of Lipopolysaccharide on Hippocampal Neurogenesis.
    Neuroimmunomodulation 2016 21;23(5-6):309-317. Epub 2017 Apr 21.
    Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, PR China.
    Objective: Aquaporin-4 (AQP4), a key molecule for water homeostasis in the brain, is associated with adult neurogenesis, but its mechanisms regulating adult neural stem cells (aNSC) remain largely unexplored. Neuroinflammation has a relevant influence on adult neurogenesis, which is a common feature in various neurodegenerative diseases. Considering the possible link between neuroinflammation and AQP4, we speculate that AQP4 may mediate the synthesis and release of proinflammatory cytokines in glia and then indirectly regulate adult hippocampal neurogenesis. Read More

    SLAMF7 is critical for phagocytosis of haematopoietic tumour cells via Mac-1 integrin.
    Nature 2017 04 19;544(7651):493-497. Epub 2017 Apr 19.
    Laboratory of Molecular Oncology, Institut de recherches cliniques de Montréal (IRCM), Montréal, Québec H2W 1R7, Canada.
    Cancer cells elude anti-tumour immunity through multiple mechanisms, including upregulated expression of ligands for inhibitory immune checkpoint receptors. Phagocytosis by macrophages plays a critical role in cancer control. Therapeutic blockade of signal regulatory protein (SIRP)-α, an inhibitory receptor on macrophages, or of its ligand CD47 expressed on tumour cells, improves tumour cell elimination in vitro and in vivo, suggesting that blockade of the SIRPα-CD47 checkpoint could be useful in treating human cancer. Read More

    Critical role for complement receptor C5aR2 in the pathogenesis of renal ischemia-reperfusion injury.
    FASEB J 2017 07 10;31(7):3193-3204. Epub 2017 Apr 10.
    Division of Nephrology, Department of Internal Medicine, University of Groningen, University Medical Centre Groningen, Groningen, The Netherlands;
    The complement system, and specifically C5a, is involved in renal ischemia-reperfusion (IR) injury. The 2 receptors for complement anaphylatoxin C5a (C5aR1 and C5aR2) are expressed on leukocytes as well as on renal epithelium. Extensive evidence shows that C5aR1 inhibition protects kidneys from IR injury; however, the role of C5aR2 in IR injury is less clear as initial studies proposed the hypothesis that C5aR2 functions as a decoy receptor. Read More

    Skin inflammation induced by lupus serum was inhibited in IL-1R deficient mice.
    Clin Immunol 2017 07 7;180:63-68. Epub 2017 Apr 7.
    Key Laboratory of Antibody Techniques of Ministry of Health, Nanjing Medical University, Nanjing, China; State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China. Electronic address:
    Skin inflammation induced by lupus serum is a useful tool to investigate the pathogenesis of lupus skin injury. IL-1 is a proinflammatory cytokine, and its role in lupus skin lesion is still unclear. We determined the role of IL-1 in lupus skin injury by using gene deficient mice. Read More

    Common genetic variants are associated with lower serum 25-hydroxyvitamin D concentrations across the year among children at northern latitudes.
    Br J Nutr 2017 Mar 6;117(6):829-838. Epub 2017 Apr 6.
    2Department of Nutrition, Exercise and Sports, Faculty of Science,University of Copenhagen,Rolighedsvej 26,DK-1958 Frederiksberg C,Denmark.
    In a longitudinal study including 642 healthy 8-11-year-old Danish children, we investigated associations between vitamin D dependent SNP and serum 25-hydroxyvitamin D (25(OH)D) concentrations across a school year (August-June). Serum 25(OH)D was measured three times for every child, which approximated measurements in three seasons (autumn, winter, spring). Dietary and supplement intake, physical activity, BMI and parathyroid hormone were likewise measured at each time point. Read More

    Systematic analysis of splicing defects in selected primary immunodeficiencies-related genes.
    Clin Immunol 2017 07 27;180:33-44. Epub 2017 Mar 27.
    Central European Institute of Technology, Masaryk University, Brno, Czech Republic; Molecular Genetics Laboratory, Centre for Cardiovascular Surgery and Transplantation, Brno, Czech Republic; Institute of Clinical Immunology and Allergology, St. Anne's University Hospital and Masaryk University, Brno, Czech Republic. Electronic address:
    Both variants affecting splice sites and those in splicing regulatory elements (SREs) can impair pre-mRNA splicing, eventually leading to severe diseases. Despite the availability of many prediction tools, prognosis of splicing affection is not trivial, especially when SREs are involved. Here, we present data on 92 in silico-/55 minigene-analysed variants detected in genes responsible for the primary immunodeficiencies development (namely BTK, CD40LG, IL2RG, SERPING1, STAT3, and WAS). Read More

    RGC-32 Promotes Th17 Cell Differentiation and Enhances Experimental Autoimmune Encephalomyelitis.
    J Immunol 2017 05 29;198(10):3869-3877. Epub 2017 Mar 29.
    Research Service, Veteran Affairs Medical Center, Baltimore, MD 21201.
    Th17 cells play a critical role in autoimmune diseases, including multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis. Response gene to complement (RGC)-32 is a cell cycle regulator and a downstream target of TGF-β that mediates its profibrotic activity. In this study, we report that RGC-32 is preferentially upregulated during Th17 cell differentiation. Read More

    Reduced PU.1 expression underlies aberrant neutrophil maturation and function in β-thalassemia mice and patients.
    Blood 2017 06 21;129(23):3087-3099. Epub 2017 Mar 21.
    Murdoch Childrens Research Institute, The Royal Children's Hospital, Parkville, VIC, Australia.
    β-Thalassemia is associated with several abnormalities of the innate immune system. Neutrophils in particular are defective, predisposing patients to life-threatening bacterial infections. The molecular and cellular mechanisms involved in impaired neutrophil function remain incompletely defined. Read More

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