Glycosylphosphatidylinositol (GPI) anchored protein deficiency serves as a reliable reporter of Pig-a gene Mutation: Support from an in vitro assay based on L5178Y/Tk(+/-) cells and the CD90.2 antigen.
- Jeffrey C Bemis,
- Svetlana L Avlasevich,
- Carson Labash,
- Page McKinzie,
- Javier Revollo,
- Vasily N Dobrovolsky,
- Stephen D Dertinger
Environ Mol Mutagen 2017 Nov 8. Epub 2017 Nov 8.
Litron Laboratories, Rochester, New York.
Lack of cell surface glycosylphosphatidylinositol (GPI)-anchored protein(s) has been used as a reporter of Pig-a gene mutation in several model systems. As an extension of this work, our laboratory initiated development of an in vitro mutation assay based on the flow cytometric assessment of CD90.2 expression on the cell surface of the mouse lymphoma cell line L5178Y/Tk(+/-) . Read More