96 results match your criteria Comparative immunology microbiology and infectious diseases[Journal]


Immunogenicity and reactogenicity of ten-valent versus 13-valent pneumococcal conjugate vaccines among infants in Ho Chi Minh City, Vietnam: a randomised controlled trial.

Lancet Infect Dis 2019 Apr 8. Epub 2019 Apr 8.

Department of Infectious Disease Epidemiology, London School of Hygiene & Tropical Medicine, London, UK; Department of Infection and Immunity, Murdoch Children's Research Institute, Melbourne, VIC, Australia; Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia.

Background: Few data are available to support the choice between the two currently available pneumococcal conjugate vaccines (PCVs), ten-valent PCV (PCV10) and 13-valent PCV (PCV13). Here we report a head-to-head comparison of the immunogenicity and reactogenicity of PCV10 and PCV13.

Methods: In this parallel, open-label, randomised controlled trial, healthy infants from two districts in Ho Chi Minh City, Vietnam, were randomly allocated (in a 3:3:5:4:5:4 ratio), with use of a computer-generated list, to one of six infant PCV schedules: PCV10 in a 3 + 1 (group A), 3 + 0 (group B), 2 + 1 (group C), or two-dose schedule (group D); PCV13 in a 2 + 1 schedule (group E); or no infant PCV (control; group F). Read More

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http://dx.doi.org/10.1016/S1473-3099(18)30734-5DOI Listing

An unusual case of Erysipelothrix rhusiopathiae prosthetic joint infection from the Canadian Arctic: whole genome sequencing unable to identify a zoonotic source.

BMC Infect Dis 2019 Mar 25;19(1):282. Epub 2019 Mar 25.

Division of Infectious Diseases, Department of Internal Medicine, Faculty of Medicine and Dentistry, University of Alberta, 3B20-11111 Jasper Avenue, Edmonton, AB, T5K 0L4, Canada.

Background: Erysipelothrix rhusiopathiae is a zoonotic pathogen that causes erysipeloid and is most frequently associated with exposure to domestic swine. Infection of native and prosthetic joints is a rarely reported manifestation.

Case Presentation: We describe a case of E. Read More

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http://dx.doi.org/10.1186/s12879-019-3913-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434803PMC
March 2019
5 Reads

Naphthylthiazoles: Targeting Multidrug-Resistant and Intracellular Staphylococcus aureus with Biofilm Disruption Activity.

ACS Infect Dis 2018 Dec 8;4(12):1679-1691. Epub 2018 Oct 8.

Department of Pharmaceutical Organic Chemistry, College of Pharmacy , Al-Azhar University , 1-Elmokhaiam Eldaem Street , Cairo 11884 , Egypt.

Thirty-two new naphthylthiazole derivatives were synthesized with the aim of exploring their antimicrobial effect on multidrug-resistant Gram-positive bacteria. Compounds 25 and 32, with ethylenediamine and methylguanidine side chains, represent the most promising derivatives, as their antibacterial spectrum includes activity against multidrug-resistant staphylococcal and enterococcal strains. Moreover, the new derivatives are highly advantageous over the existing frontline therapeutics for the treatment of multidrug-resistant Gram-positive bacteria. Read More

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http://pubs.acs.org/doi/10.1021/acsinfecdis.8b00172
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http://dx.doi.org/10.1021/acsinfecdis.8b00172DOI Listing
December 2018
3 Reads

Antibody responses against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis in children with acute respiratory infection with or without nasopharyngeal bacterial carriage.

Infect Dis (Lond) 2018 09 24;50(9):705-713. Epub 2018 Apr 24.

d Postgraduate Programme in Health Sciences, Department of Paediatrics , Federal University of Bahia School of Medicine , Salvador , Brazil.

Background: We studied Immunoglobulin G (IgG) antibody responses against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis in young children with acute viral type respiratory infection and analyzed the findings in a multivariate model including age, nasopharyngeal carriage of the tested bacteria and pneumococcal vaccination.

Methods: We included 227 children aged 6-23 months with acute respiratory infection. Nasopharyngeal aspirates were tested for bacterial carriage through detection of messenger RNA (mRNA) transcript with nCounter analysis. Read More

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https://www.tandfonline.com/doi/full/10.1080/23744235.2018.1
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http://dx.doi.org/10.1080/23744235.2018.1463451DOI Listing
September 2018
23 Reads

Comparison of hepatitis E virus seroprevalence between HBsAg-positive population and healthy controls in Shandong province, China.

BMC Infect Dis 2018 02 12;18(1):75. Epub 2018 Feb 12.

Academy of Preventive Medicine, Shandong University, Jinan, China.

Background: Persons with chronic hepatitis B (CHB) infection were reported to suffer severe disease after hepatitis E virus (HEV) superinfection, but the studies regarding HEV seroprevalence in this population were limited. A recent study in Vietnam found higher HEV seroprevalence among CHB patients compared with healthy controls.

Methods: A community-based case-control study was conducted in two counties of Shandong province, China, where hepatitis E incidence was at the highest (Rushan) and lowest (Zhangqiu) in the province based on data from routine public health surveillance. Read More

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http://dx.doi.org/10.1186/s12879-018-2974-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810058PMC
February 2018
5 Reads

Evaluation of fluorescent in-situ hybridization technique for diagnosis of malaria in Ahero Sub-County hospital, Kenya.

BMC Infect Dis 2018 01 8;18(1):22. Epub 2018 Jan 8.

Department of Medical Microbiology, College of Health Sciences, University of Nairobi, Nairobi, Kenya.

Background: Malaria is a major cause of morbidity and mortality. Treatment of malaria in a timely manner could avert deaths. Treatment ultimately relies on the rapid and accurate diagnosis. Read More

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http://dx.doi.org/10.1186/s12879-017-2875-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5759278PMC
January 2018
26 Reads

Incident Mycobacterium tuberculosis infection in household contacts of infectious tuberculosis patients in Brazil.

BMC Infect Dis 2017 08 18;17(1):576. Epub 2017 Aug 18.

Núcleo de Doenças Infecciosas, Universidade Federal do Espírito Santo (UFES), Vitória, Brazil.

Background: In household contact investigations of tuberculosis (TB), a second tuberculin skin test (TST) obtained several weeks after a first negative result consistently identifies individuals that undergo TST conversion. It remains unclear whether this delay in M. tuberculosis infection is related to differences in the infectious exposure, TST boosting, partial host resistance, or some other factor. Read More

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http://dx.doi.org/10.1186/s12879-017-2675-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563014PMC
August 2017
26 Reads

Designing Probes for Immunodiagnostics: Structural Insights into an Epitope Targeting Burkholderia Infections.

ACS Infect Dis 2017 10 21;3(10):736-743. Epub 2017 Jul 21.

Department of Biosciences, Università degli Studi di Milano , Via Celoria 26, Milano, 20133, Italy.

Structure-based epitope prediction drives the design of diagnostic peptidic probes to reveal specific antibodies elicited in response to infections. We previously identified a highly immunoreactive epitope from the peptidoglycan-associated lipoprotein (Pal) antigen from Burkholderia pseudomallei, which could also diagnose Burkholderia cepacia infections. Here, considering the high phylogenetic conservation within Burkholderia species, we ask whether cross-reactivity can be reciprocally displayed by the synthetic epitope from B. Read More

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http://dx.doi.org/10.1021/acsinfecdis.7b00080DOI Listing
October 2017
12 Reads

Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study.

BMC Infect Dis 2017 05 15;17(1):344. Epub 2017 May 15.

Graduate School of Health Science Studies, Kibi International University, 8 Igamachi, Takahashi, 716-8508, Japan.

Background: Strains of the Beijing genotype of Mycobacterium tuberculosis (MTB) are reportedly associated with the virulence of tuberculosis (TB) infection, unfavorable outcomes of anti-TB treatment, and the global TB pandemic. Rv0679c, a hypothetical membrane protein related to host cell invasion, has a Beijing genotype-specific mutation at residue 142 (Asn142Lys). Antigenicity differences between Rv0679c-Asn142 (N-type) and Rv0679c-Lys142 (K-type) have been previously observed in mice antigen-antibody responses. Read More

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http://dx.doi.org/10.1186/s12879-017-2442-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433171PMC
May 2017
42 Reads
2.613 Impact Factor

Comparing tuberculosis management under public and private healthcare providers: Victoria, Australia, 2002-2015.

BMC Infect Dis 2017 05 3;17(1):324. Epub 2017 May 3.

Victorian Tuberculosis Program, The Peter Doherty Institute for Infection and Immunity, Victoria, Australia.

Background: Private healthcare providers are important to tuberculosis (TB) management globally, although internationally there are reports of suboptimal management and disparities in treatment commencement in the private sector. We compared the management of TB patients receiving private versus public healthcare in Victoria, an industrialised setting with low tuberculosis (TB) incidence.

Methods: Retrospective cohort study: 2002-2015. Read More

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http://dx.doi.org/10.1186/s12879-017-2421-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415803PMC
May 2017
13 Reads

Comparison of two commercial broad-range PCR and sequencing assays for identification of bacteria in culture-negative clinical samples.

BMC Infect Dis 2017 03 27;17(1):233. Epub 2017 Mar 27.

Department of Clinical Microbiology, Centre for Diagnostics, Rigshospitalet, Copenhagen, Denmark.

Background: Culturing has long been the gold standard for detecting aetiologic agents in bacterial infections. In some cases, however, culturing fails to detect the infection. To further investigate culture-negative samples, amplification and subsequent sequencing of the 16S rRNA gene is often applied. Read More

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http://dx.doi.org/10.1186/s12879-017-2333-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5368927PMC
March 2017
13 Reads

Comparison of outcome and clinical characteristics of bacteremia caused by methicillin-resistant, penicillin-resistant and penicillin-susceptible Staphylococcus aureus strains.

Infect Dis (Lond) 2017 Jul 28;49(7):493-500. Epub 2017 Feb 28.

a Department of Internal Medicine , Infectious Disease Unit, Tampere University Hospital , Tampere , Finland.

Background: The aim of this study was to assess the association of methicillin resistance and penicillinase production with clinical characteristics and outcome of Staphylococcus aureus bacteremia.

Methods: For 126 patients with methicillin-resistant Staphylococcus aureus (MRSA) bacteremia, 378 age- and gender-matched controls with methicillin-susceptible Staphylococcus aureus (MSSA) bacteremia were selected. Of controls, 126 had bacteremia caused by penicillin-susceptible strains (PSSA) and 252 by penicillinase-producing strains (PRSA). Read More

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https://www.tandfonline.com/doi/full/10.1080/23744235.2017.1
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http://dx.doi.org/10.1080/23744235.2017.1292046DOI Listing
July 2017
13 Reads

Comparing culture and molecular methods for the identification of microorganisms involved in necrotizing soft tissue infections.

BMC Infect Dis 2016 11 8;16(1):652. Epub 2016 Nov 8.

Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Denmark.

Background: Necrotizing soft tissue infections (NSTIs) are a group of infections affecting all soft tissues. NSTI involves necrosis of the afflicted tissue and is potentially life threatening due to major and rapid destruction of tissue, which often leads to septic shock and organ failure. The gold standard for identification of pathogens is culture; however molecular methods for identification of microorganisms may provide a more rapid result and may be able to identify additional microorganisms that are not detected by culture. Read More

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http://dx.doi.org/10.1186/s12879-016-1976-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5100109PMC
November 2016
19 Reads
2.613 Impact Factor

Comparative M-protein analysis of Streptococcus pyogenes from pharyngitis and skin infections in New Zealand: Implications for vaccine development.

BMC Infect Dis 2016 10 12;16(1):561. Epub 2016 Oct 12.

School of Medical Sciences and Maurice Wilkins Centre, University of Auckland, Auckland, New Zealand.

Background: Acute rheumatic fever (ARF) and rheumatic heart disease (RHD) are responsible for a significant disease burden amongst Māori and Pacific populations in New Zealand (NZ). However, contemporary data are lacking regarding circulating group A Streptococcal (GAS) strains in NZ. Such information is important in guiding vaccine development. Read More

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http://dx.doi.org/10.1186/s12879-016-1891-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5062888PMC
October 2016
33 Reads

Measles seroprevalence, outbreaks, and vaccine coverage in Rwanda.

Infect Dis (Lond) 2016 Nov-Dec;48(11-12):800-7. Epub 2016 Jul 8.

c Department of Infectious Diseases , Institute of Biomedicine, University of Gothenburg , Sweden ;

Background: Measles outbreaks are reported after insufficient vaccine coverage, especially in countries recovering from natural disaster or conflict. We compared seroprevalence to measles in blood donors in Rwanda and Sweden and explored distribution of active cases of measles and vaccine coverage in Rwanda.

Methods: 516 Rwandan and 215 Swedish blood donors were assayed for measles-specific immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA). Read More

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http://dx.doi.org/10.1080/23744235.2016.1201720DOI Listing
February 2017
26 Reads

Clinical and epidemiological analysis of Campylobacter fetus subsp. fetus infections in humans and comparative genetic analysis with strains isolated from cattle.

BMC Infect Dis 2016 May 14;16:198. Epub 2016 May 14.

Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

Background: Campylobacter fetus subspecies fetus (CFF) is an important pathogen for both cattle and humans. We performed a systematic epidemiological and clinical study of patients and evaluated the genetic relatedness of 17 human and 17 bovine CFF isolates by using different genotyping methods. In addition, the serotype, the dissemination of the genomic island containing a type IV secretion system (T4SS) and resistance determinants for tetracycline and streptomycin were also evaluated. Read More

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http://dx.doi.org/10.1186/s12879-016-1538-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868008PMC
May 2016
13 Reads

Comparison of performance of two Treponema pallidum automated chemiluminescent immunoassays in blood donors.

Infect Dis (Lond) 2016 9;48(6):483-7. Epub 2016 Feb 9.

a U.O.C. Immunohematology, Transfusion Medicine and Transplant Immunology, Department of Internal and Specialty Medicine , Azienda Ospedaliera Universitaria (AOU), Second University of Naples , Naples , Italy ;

The recrudescence of syphilis is leading to the development of new serological tests. The goal of this study was to compare the performance of the more recent Elecsys Syphilis assay, the Electro Chemiluminescence Immunoassay (ECLIA), with the former Architect Syphilis TP assay, the Chemiluminescent Microparticle Immunoassay (CMIA), for the detection of antibodies against Treponema pallidum in blood donors. Serum samples of 5543 voluntary blood donors were screened in parallel with two tests. Read More

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http://dx.doi.org/10.3109/23744235.2016.1142674DOI Listing
January 2017
24 Reads

Comparative performance characteristics of the urine lipoarabinomannan strip test and sputum smear microscopy in hospitalized HIV-infected patients with suspected tuberculosis in Harare, Zimbabwe.

BMC Infect Dis 2016 Jan 22;16:20. Epub 2016 Jan 22.

Lung Infection and Immunity Unit, Division of Pulmonology, Department of Medicine, University of Cape Town, Cape Town, South Africa.

Background: In Zimbabwe, sputum smear microscopy (SSM) is the routinely used TB diagnostic tool in hospitalised HIV-infected patients. However, SSM has poor sensitivity in HIV-infected patients. We compared performance of urine lipoarabinomannan strip test (LAM) and SSM among hospitalized HIV-infected patients with suspected TB. Read More

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http://dx.doi.org/10.1186/s12879-016-1339-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4722705PMC
January 2016
62 Reads
4 Citations
2.613 Impact Factor

Immunogenicity and safety of a novel monovalent high-dose inactivated poliovirus type 2 vaccine in infants: a comparative, observer-blind, randomised, controlled trial.

Lancet Infect Dis 2016 Mar 21;16(3):321-30. Epub 2015 Dec 21.

Bill & Melinda Gates Foundation, Seattle, WA, USA. Electronic address:

Background: Following the proposed worldwide switch from trivalent oral poliovirus vaccine (tOPV) to bivalent types 1 and 3 OPV (bOPV) in 2016, inactivated poliovirus vaccine (IPV) will be the only source of protection against poliovirus type 2. With most countries opting for one dose of IPV in routine immunisation schedules during this transition because of cost and manufacturing constraints, optimisation of protection against all poliovirus types will be a priority of the global eradication programme. We assessed the immunogenicity and safety of a novel monovalent high-dose inactivated poliovirus type 2 vaccine (mIPV2HD) in infants. Read More

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http://dx.doi.org/10.1016/S1473-3099(15)00488-0DOI Listing
March 2016
22 Reads
9 Citations
22.433 Impact Factor

Evaluation of sensitivity and specificity of ELISA against Widal test for typhoid diagnosis in endemic population of Kathmandu.

BMC Infect Dis 2015 Nov 14;15:523. Epub 2015 Nov 14.

Central Department of Biotechnology, Tribhuvan University, Kirtipur, Kathmandu, Nepal.

Background: Widal test, which has poor predictive outcomes in predominant typhoid population, is not standard enough to predict accurate diagnosis. This study aims to compare the diagnostic accuracy of Widal test to ELISA using blood culture as gold standard.

Methods: The blood samples were collected in Capital Hospital, Kathmandu, Nepal from febrile patients having ≥48 h fever in 3 years study period for blood culture, Widal test and IgG-IgM ELISA. Read More

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http://dx.doi.org/10.1186/s12879-015-1248-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647669PMC
November 2015
33 Reads
2 Citations
2.613 Impact Factor

Characteristics and spread to the native population of HIV-1 non-B subtypes in two European countries with high migration rate.

BMC Infect Dis 2015 Nov 16;15:524. Epub 2015 Nov 16.

Aids Reference Laboratory, Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, De Pintelaan 185-Blok A, B-9000, Ghent, Belgium.

Background: Non-B subtypes account for at least 50 % of HIV-1 infections diagnosed in Belgium and Luxembourg. They are considered to be acquired through heterosexual contacts and infect primarily individuals of foreign origin. Information on the extent to which non-B subtypes spread to the local population is incomplete. Read More

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http://dx.doi.org/10.1186/s12879-015-1217-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647655PMC
November 2015
14 Reads

Topical imiquimod before intradermal trivalent influenza vaccine for protection against heterologous non-vaccine and antigenically drifted viruses: a single-centre, double-blind, randomised, controlled phase 2b/3 trial.

Lancet Infect Dis 2016 Feb 9;16(2):209-18. Epub 2015 Nov 9.

Carol Yu Centre for Infection and Division of Infectious Diseases, University of Hong Kong, Hong Kong Special Administrative Region, China; Department of Microbiology, University of Hong Kong, Hong Kong Special Administrative Region, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, China. Electronic address:

Background: Pretreatment with topical imiquimod, a synthetic agonist of toll-like receptor 7, significantly improved the immunogenicity of influenza vaccination in elderly people. We aimed to clarify its effect in a younger age group.

Methods: In this double-blind, randomised controlled trial, we enrolled healthy volunteers aged 18-30 years in early 2014 to receive the 2013-14 northern-hemisphere winter trivalent influenza vaccine at the Queen Mary Hospital, (Hong Kong, China). Read More

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http://dx.doi.org/10.1016/S1473-3099(15)00354-0DOI Listing
February 2016
37 Reads

Antifungal prophylaxis with liposomal amphotericin B and caspofungin in high-risk patients after liver transplantation: impact on fungal infections and immune system.

Infect Dis (Lond) 2016 Feb;48(2):161-6

c CEB-Liver Transplant Unit , Hospital A. Cardarelli .

Antifungal prophylaxis may be required in high-risk patients undergoing liver transplantation and for that reason we aimed to verify its role and its related impact on the graft. From January 2006 throughout 2012, 250 liver transplants were evaluated and 54 patients identified as being at higher risk were randomly selected to undergo the following schedule: 28 patients received liposomal amphotericin B and 26 received caspofungin. We evaluated, throughout 12 months, renal and liver function tests, bacterial and fungal infection episodes, and intensive care unit (ICU) stay, as well as the Th1 and Th2 cytokine network. Read More

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http://dx.doi.org/10.3109/23744235.2015.1100322DOI Listing
February 2016
5 Reads

Assessing the impact of pneumococcal conjugate vaccines on invasive pneumococcal disease using polymerase chain reaction-based surveillance: an experience from South Africa.

BMC Infect Dis 2015 Oct 26;15:450. Epub 2015 Oct 26.

Centre for Respiratory Diseases and Meningitis, National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, South Africa.

Background: The use of molecular diagnostic techniques for the evaluation of the impact of pneumococcal conjugate vaccines (PCVs) has not been documented. We aimed to evaluate the impact of PCVs on invasive pneumococcal disease (IPD) using polymerase chain reaction (PCR)-based techniques and compare with results obtained from culture-based methods.

Methods: We implemented two independent surveillance programs for IPD among individuals hospitalized at one large surveillance site in Soweto, South Africa during 2009-2012: (i) PCR-based (targeting the lytA gene) syndromic pneumonia surveillance; and (ii) culture-based laboratory surveillance. Read More

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http://www.biomedcentral.com/1471-2334/15/450
Publisher Site
http://dx.doi.org/10.1186/s12879-015-1198-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620746PMC
October 2015
45 Reads

Development and comparative evaluation of two antigen detection tests for Visceral Leishmaniasis.

BMC Infect Dis 2015 Sep 22;15:384. Epub 2015 Sep 22.

Infectious Disease Research Institute, 1616 Eastlake Ave E, Seattle, WA, 98102, USA.

Background: Visceral leishmaniasis (VL) can be fatal without timely diagnosis and treatment. Treatment efficacies vary due to drug resistance, drug toxicity and co-morbidities. It is important to monitor treatment responsiveness to confirm cure and curtail relapse. Read More

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http://dx.doi.org/10.1186/s12879-015-1125-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4580298PMC
September 2015
24 Reads

Genetic markers, genotyping methods & next generation sequencing in Mycobacterium tuberculosis.

Indian J Med Res 2015 Jun;141(6):761-74

Department of Immunology, National Institute of Research in Tuberculosis (ICMR), Chennai, India.

Molecular epidemiology (ME) is one of the main areas in tuberculosis research which is widely used to study the transmission epidemics and outbreaks of tubercle bacilli. It exploits the presence of various polymorphisms in the genome of the bacteria that can be widely used as genetic markers. Many DNA typing methods apply these genetic markers to differentiate various strains and to study the evolutionary relationships between them. Read More

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http://dx.doi.org/10.4103/0971-5916.160695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525401PMC
June 2015
10 Reads

Changing risk awareness and personal protection measures for low to high pathogenic avian influenza in live-poultry markets in Taiwan, 2007 to 2012.

BMC Infect Dis 2015 Jun 24;15:241. Epub 2015 Jun 24.

Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, 17 Xu-Zhou Road, Taipei (100), Taiwan.

Background: Outbreaks of low and high pathogenic avian influenza (LPAI, HPAI) H5N2 in chickens have occurred in Taiwan since 2003 and 2012, respectively. Fully understanding the different awareness, attitudes and protective behaviors adopted by workers in live-poultry markets (LPMWs) and local community residents (CRs) to face the challenges of LPAI and HPAI is very important to minimize viral adaptations to human populations.

Methods: A structural questionnaire containing information on respondents' occupation, personal risk awareness, attitudes toward different policies, and preventative measures was administered. Read More

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http://bmcinfectdis.biomedcentral.com/articles/10.1186/s1287
Publisher Site
http://dx.doi.org/10.1186/s12879-015-0987-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4478710PMC
June 2015
11 Reads

Dual treatment with lopinavir-ritonavir plus lamivudine versus triple treatment with lopinavir-ritonavir plus lamivudine or emtricitabine and a second nucleos(t)ide reverse transcriptase inhibitor for maintenance of HIV-1 viral suppression (OLE): a randomised, open-label, non-inferiority trial.

Lancet Infect Dis 2015 Jul 7;15(7):785-92. Epub 2015 Jun 7.

Hospital Clínic/Institut d'Investigacions Biomèdiques August Pi i Sunyer, University of Barcelona, Barcelona, Spain.

Background: Our objective was to assess therapeutic non-inferiority of dual treatment with lopinavir-ritonavir and lamivudine to triple treatment with lopinavir-ritonavir plus two nucleos(t)ides for maintenance of HIV-1 viral suppression.

Methods: In this randomised, open-label, non-inferiority trial, we recruited patients from 32 HIV units in hospitals in Spain and France. Eligible patients were HIV-infected adults (aged ≥18 years) with HIV-1 RNA of less than 50 copies per mL, for at least 6 months on triple treatment with lopinavir-ritonavir (twice daily) plus lamivudine or emtricitabine and a second nucleos(t)ide, with no resistance or virological failure to these drugs, and no positive hepatitis B serum surface antigen. Read More

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http://dx.doi.org/10.1016/S1473-3099(15)00096-1DOI Listing
July 2015
29 Reads

Detection and serotyping of pneumococci in community acquired pneumonia patients without culture using blood and urine samples.

BMC Infect Dis 2015 Feb 13;15:56. Epub 2015 Feb 13.

Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment (RIVM), Laboratory for Infectious Diseases and Perinatal Screening, Antonie van Leeuwenhoeklaan 9, P.O.Box 1, 3720 BA, Bilthoven, The Netherlands.

Background: Treatment of community acquired pneumonia (CAP) patients with antibiotics before laboratory-confirmed diagnosis leads to loss of knowledge on the causative bacterial pathogen. Therefore, an increasing number of pneumococcal infections is identified using non-culture based techniques. However, methods for serotyping directly on the clinical specimen remain scarce. Read More

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http://dx.doi.org/10.1186/s12879-015-0788-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330648PMC
February 2015
12 Reads

Predictors of positive or negative legionella urinary antigen test in community-acquired pneumonia.

Infect Dis (Lond) 2015 Jul 11;47(7):484-90. Epub 2015 Mar 11.

Department of Infectious Diseases, Aalborg University Hospital.

Background: Legionella pneumonia remains a diagnostic challenge. The legionella urinary antigen test (LUT) primarily detects Legionella pneumophila serogroup 1, accounting for 64% of Danish cases, and is often the only legionella test performed. We aimed to identify variables predictive of a positive or negative test result and to explore how the LUT was used in clinical practice. Read More

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http://dx.doi.org/10.3109/23744235.2015.1021830DOI Listing
July 2015
6 Reads

Immunogenicity, safety, and tolerability of a recombinant measles-virus-based chikungunya vaccine: a randomised, double-blind, placebo-controlled, active-comparator, first-in-man trial.

Lancet Infect Dis 2015 May 2;15(5):519-27. Epub 2015 Mar 2.

Viral Genomics and Vaccination Unit, Institut Pasteur, Paris, CNRS UMR-3569, France.

Background: Chikungunya is an emerging arthropod-borne disease that has spread from tropical endemic areas to more temperate climates of the USA and Europe. However, no specific treatment or preventive measure is yet available. We aimed to investigate the immunogenicity and safety of a live recombinant measles-virus-based chikungunya vaccine. Read More

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http://dx.doi.org/10.1016/S1473-3099(15)70043-5DOI Listing
May 2015
15 Reads

Temporal cytokine expression and the target organ attributes unravel novel aspects of autoimmune arthritis.

Indian J Med Res 2013 Nov;138(5):717-31

Department of Microbiology & Immunology, University of Maryland School of Medicine, Baltimore, MD, USA.

Susceptibility to autoimmunity is determined by multiple factors. Defining the contribution of the quantitative versus qualitative aspects of antigen-directed immune responses as well as the factors influencing target organ susceptibility is vital to advancing the understanding of the pathogenesis of autoimmunity. In a series of studies, we have addressed these issues using the adjuvant-induced arthritis (AA) model of human rheumatoid arthritis (RA). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3928702PMC
November 2013
7 Reads

CCL3L1 copy number, HIV load, and immune reconstitution in sub-Saharan Africans.

BMC Infect Dis 2013 Nov 12;13:536. Epub 2013 Nov 12.

Department of Genetics, University of Leicester, University Road, Leicester, LE1 7RH, UK.

Background: The role of copy number variation of the CCL3L1 gene, encoding MIP1α, in contributing to the host variation in susceptibility and response to HIV infection is controversial. Here we analyse a sub-Saharan African cohort from Tanzania and Ethiopia, two countries with a high prevalence of HIV-1 and a high co-morbidity of HIV with tuberculosis.

Methods: We use a form of quantitative PCR called the paralogue ratio test to determine CCL3L1 gene copy number in 1134 individuals and validate our copy number typing using array comparative genomic hybridisation and fiber-FISH. Read More

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http://dx.doi.org/10.1186/1471-2334-13-536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829100PMC
November 2013
17 Reads
8 Citations
2.613 Impact Factor

Extended antigen sparing potential of AS03-adjuvanted pandemic H1N1 vaccines in children, and immunological equivalence of two formulations of AS03-adjuvanted H1N1 vaccines: results from two randomised trials.

BMC Infect Dis 2013 Sep 16;13:435. Epub 2013 Sep 16.

Inserm, CIC BT505; Assistance Publique-Hôpitaux de Paris, Groupe Hospitalier Cochin Broca Hôtel-Dieu, CIC de vaccinologie Cochin Pasteur; Université Paris Descartes, Paris, France.

Background: Pandemic influenza vaccine manufacturing capacity and distribution agility is enhanced through the availability of equivalent antigen-sparing vaccines. We evaluated equivalence in terms of immunogenicity between GlaxoSmithKline Vaccines' A/California/7/2009 (H1N1)v-like-AS03 vaccines manufactured in Dresden (D-Pan), and Quebec (Q-Pan).

Methods: In two studies, 334 adults 18-60 years of age received 2 doses of D-Pan or Q-Pan containing 3. Read More

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http://dx.doi.org/10.1186/1471-2334-13-435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848562PMC
September 2013
33 Reads

Differential arthritogenicity of Staphylococcus aureus strains isolated from biological samples.

BMC Infect Dis 2013 Aug 30;13:400. Epub 2013 Aug 30.

Department of Microbiology and Immunology, Biosciences Institute, Univ, Estadual Paulista (UNESP), Distrito de Rubião Júnior s/n, 18618-070 Botucatu, São Paulo, Brazil.

Background: Staphylococcus aureus is the most common agent of septic arthritis that is a severe, rapidly progressive and destructive joint disease. Superantigens produced by S. aureus are considered the major arthritogenic factors. Read More

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http://dx.doi.org/10.1186/1471-2334-13-400DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3846911PMC
August 2013
9 Reads

Envelope protein gene based molecular characterization of Japanese encephalitis virus clinical isolates from West Bengal, India: a comparative approach with respect to SA14-14-2 live attenuated vaccine strain.

BMC Infect Dis 2013 Aug 8;13:368. Epub 2013 Aug 8.

Background: Increasing virulence of Japanese encephalitis virus (JEV), a mosquito-borne zoonotic pathogen is of grave concern because it causes a neurotrophic killer disease Japanese Encephalitis (JE) which, in turn, is responsible globally for viral acute encephalitis syndrome (AES). Despite the availability of vaccine, JE/AES cases and deaths have become regular features in the different rural districts of West Bengal (WB) state, India, indicating either the partial coverage of vaccine or the emergence of new strain of JEV. Therefore, a study was undertaken to characterize and compare the complete envelope (E) protein gene based molecular changes/patterns of JEVs circulating in WB. Read More

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http://dx.doi.org/10.1186/1471-2334-13-368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751164PMC
August 2013
6 Reads

MicroRNA regulation and its effects on cellular transcriptome in human immunodeficiency virus-1 (HIV-1) infected individuals with distinct viral load and CD4 cell counts.

BMC Infect Dis 2013 May 30;13:250. Epub 2013 May 30.

Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, 425 Parran Hall, 130 Desoto Street, Pittsburgh, PA 15261, USA.

Background: Disease progression in the absence of therapy varies significantly in HIV-1 infected individuals. Both viral and host cellular molecules are implicated; however, the exact role of these factors and/or the mechanism involved remains elusive. To understand how microRNAs (miRNAs), which are regulators of transcription and translation, influence host cellular gene expression (mRNA) during HIV-1 infection, we performed a comparative miRNA and mRNA microarray analysis using PBMCs obtained from infected individuals with distinct viral load and CD4 counts. Read More

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http://dx.doi.org/10.1186/1471-2334-13-250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680326PMC
May 2013
13 Reads

Strategies to increase responsiveness to hepatitis B vaccination in adults with HIV-1.

Lancet Infect Dis 2012 Dec;12(12):966-76

Division of Infectious Diseases, Emory University School of Medicine, The Hope Clinic of the Emory Vaccine Center, Decatur, GA, USA.

HIV and hepatitis B virus co-infection leads to substantially increased morbidity and mortality compared with either infection alone. Immunisation with hepatitis B virus vaccine is the most effective way to prevent the infection in people with HIV; however, these patients have decreased vaccine responses and a short duration of protection compared with immunocompetent individuals. Control of HIV replication with highly active antiretroviral therapy and increased CD4 cell counts are associated with improved immune responses to hepatitis B vaccination. Read More

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http://dx.doi.org/10.1016/S1473-3099(12)70243-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141523PMC
December 2012
8 Reads

Cross-protective efficacy of two human papillomavirus vaccines: a systematic review and meta-analysis.

Lancet Infect Dis 2012 Oct 22;12(10):781-9. Epub 2012 Aug 22.

Unité de Recherche en Santé des Populations (URESP), Centre de Recherche Fonds de la Recherche en Santé du Québec (FRSQ) du Centre Hospitalier Affilié Universitaire de Québec (CHA), Quebec, Canada.

Background: The extent of cross-protection is a key element in the choice of human papillomavirus (HPV) vaccine to use in vaccination programmes. We compared the cross-protective efficacy of the bivalent vaccine (HPV 16 and 18; Cervarix, GlaxoSmithKline Biologicals, Rixensart, Belgium) and quadrivalent vaccine (HPV 6, 11, 16, and 18; Gardasil, Merck, Whitehouse Station, NJ, USA) against non-vaccine type HPVs.

Methods: We searched Medline and Embase databases, conference abstracts, and manufacturers' websites for randomised clinical trials assessing the efficacy of bivalent and quadrivalent vaccines against persistent infections (lasting ≥6 months) and cervical intraepithelial neoplasia (CIN) associated with the non-vaccine type HPVs (types 31, 33, 45, 52, and 58). Read More

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http://dx.doi.org/10.1016/S1473-3099(12)70187-1DOI Listing
October 2012
7 Reads

Loss of CD127 & increased immunosenescence of T cell subsets in HIV infected individuals.

Indian J Med Res 2011 Dec;134(6):972-81

Department of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.

Background & Objectives: HIV infection is characterized by a perturbation in T cell homeostasis, leading to alteration in T cell subsets. In addition to alteration in differentiation, HIV infection also leads to change in T cell survival and regenerative capacity, as suggested by differential expression of CD127 and CD57. We evaluated the expression patterns of CD127 and CD57 on CD4 and CD8 effector, memory and naïve T cell subsets in HIV-infected and uninfected individuals. Read More

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http://dx.doi.org/10.4103/0971-5916.92645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284107PMC
December 2011
7 Reads

Treatment outcomes in HIV-infected adolescents attending a community-based antiretroviral therapy clinic in South Africa.

BMC Infect Dis 2012 Jan 25;12:21. Epub 2012 Jan 25.

The Desmond Tutu HIV Centre, Institute for Infectious Disease and Molecular Medicine, and the Department of Medicine, Faculty of Health Sciences, University of Cape Town, Anzio Road, Observatory, 7925 Cape Town, South Africa.

Background: Very few data are available on treatment outcomes of adolescents living with HIV infection (whether perinatally acquired or sexually acquired) in sub-Saharan Africa. The present study therefore compared the treatment outcomes in adolescents with those of young adults at a public sector community-based ART programme in Cape Town, South Africa.

Methods: Treatment outcomes of adolescents (9-19 years) were compared with those of young adults (20-28 years), enrolled in a prospective cohort between September 2002 and June 2009. Read More

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http://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-
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http://dx.doi.org/10.1186/1471-2334-12-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3295677PMC
January 2012
10 Reads

Immunogenicity of supplemental doses of poliovirus vaccine for children aged 6-9 months in Moradabad, India: a community-based, randomised controlled trial.

Lancet Infect Dis 2012 Feb 7;12(2):128-35. Epub 2011 Nov 7.

Global Immunization Division, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.

Background: The continued presence of polio in northern India poses challenges to the interruption of wild poliovirus transmission and the management of poliovirus risks in the post-eradication era. We aimed to assess the current immunity profile after routine doses of trivalent oral poliovirus vaccine (OPV) and numerous supplemental doses of type-1 monovalent OPV (mOPV1), and compared the effect of five vaccine formulations and dosages on residual immunity gaps.

Methods: We did a community-based, randomised controlled trial of healthy infants aged 6-9 months at ten sites in Moradabad, India. Read More

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http://linkinghub.elsevier.com/retrieve/pii/S147330991170190
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http://dx.doi.org/10.1016/S1473-3099(11)70190-6DOI Listing
February 2012
12 Reads
22.433 Impact Factor

Efficacy and safety of once daily elvitegravir versus twice daily raltegravir in treatment-experienced patients with HIV-1 receiving a ritonavir-boosted protease inhibitor: randomised, double-blind, phase 3, non-inferiority study.

Lancet Infect Dis 2012 Jan 18;12(1):27-35. Epub 2011 Oct 18.

Hôpital Saint Louis and University of Paris Diderot, Paris, France.

Background: Elvitegravir is a once daily inhibitor of HIV-1 integrase boosted by ritonavir. We aimed to compare the efficacy and safety of elvitegravir with raltegravir, another HIV-1 integrase inhibitor, in patients in whom previous antiretroviral treatment failed.

Methods: We conducted a randomised, double-blind, double-dummy, phase 3 study at 234 sites in 13 countries. Read More

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http://dx.doi.org/10.1016/S1473-3099(11)70249-3DOI Listing
January 2012
24 Reads

Immunomodulatory factors in cervicovaginal secretions from pregnant and non-pregnant women: a cross-sectional study.

BMC Infect Dis 2011 Sep 30;11:263. Epub 2011 Sep 30.

Department of Plant Pathology and Microbiology, University of California, Riverside, USA.

Background: Pregnant women are at an increased risk for HIV infection due to unknown biological causes. Given the strong effect of sex-hormones on the expression of immunomuodulatory factors, the central role of mucosal immunity in HIV pathogenesis and the lack of previous studies, we here tested for differences in immunomuodulatory factors in cervico-vaginal secretions between pregnant and non-pregnant women.

Methods: We compared concentrations of 39 immunomodulatory factors in cervicovaginal lavages (CVL) from 21 pregnant women to those of 24 non-pregnant healthy women from the US. Read More

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http://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-
Publisher Site
http://dx.doi.org/10.1186/1471-2334-11-263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3190379PMC
September 2011
15 Reads

Monitoring of HIV viral loads, CD4 cell counts, and clinical assessments versus clinical monitoring alone for antiretroviral therapy in rural district hospitals in Cameroon (Stratall ANRS 12110/ESTHER): a randomised non-inferiority trial.

Lancet Infect Dis 2011 Nov 8;11(11):825-33. Epub 2011 Aug 8.

Institut de Recherche pour le Développement (IRD), University Montpellier 1, UMI 233, Montpellier, France.

Background: Scaling up of antiretroviral therapy in low-resource countries is done on the basis of decentralised, integrated HIV care in rural facilities; however, laboratory monitoring is generally unavailable. We aimed to assess the effectiveness and safety of clinical monitoring alone (CLIN) in terms of non-inferiority to laboratory and clinical monitoring (LAB).

Methods: We did a randomised, open-label, non-inferiority trial in nine rural district hospitals in Cameroon. Read More

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http://dx.doi.org/10.1016/S1473-3099(11)70168-2DOI Listing
November 2011
42 Reads

Assessment and comparative analysis of a rapid diagnostic test (Tubex®) for the diagnosis of typhoid fever among hospitalized children in rural Tanzania.

BMC Infect Dis 2011 May 24;11:147. Epub 2011 May 24.

Translational Research Division, International Vaccine Institute, Seoul, Korea.

Background: Typhoid fever remains a significant health problem in many developing countries. A rapid test with a performance comparable to that of blood culture would be highly useful. A rapid diagnostic test for typhoid fever, Tubex®, is commercially available that uses particle separation to detect immunoglobulin M directed towards Salmonella Typhi O9 lipopolysaccharide in sera. Read More

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http://dx.doi.org/10.1186/1471-2334-11-147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3123569PMC
May 2011
25 Reads
12 Citations
2.613 Impact Factor

Evaluation of envelope domain III-based single chimeric tetravalent antigen and monovalent antigen mixtures for the detection of anti-dengue antibodies in human sera.

BMC Infect Dis 2011 Mar 15;11:64. Epub 2011 Mar 15.

International Centre for Genetic Engineering & Biotechnology, New Delhi, India.

Background: Flavivirus cross-reactive antibodies in human sera interfere with the definitive identification of dengue virus (DENV) infections especially in areas with multiple co-circulating flaviviruses. Use of DENV envelope domain-III (EDIII) can partially resolve the problem. This study has examined the effect of (i) incorporating the EDIIIs of four DENV serotypes into a single chimeric antigen, and (ii) immobilizing the antigen through specific interaction on the sensitivity and specificity of anti-DENV antibody detection. Read More

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http://dx.doi.org/10.1186/1471-2334-11-64DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068959PMC
March 2011
20 Reads

Immunogenicity and safety of a two-dose schedule of whole-virion and AS03A-adjuvanted 2009 influenza A (H1N1) vaccines: a randomised, multicentre, age-stratified, head-to-head trial.

Lancet Infect Dis 2011 Feb 16;11(2):91-101. Epub 2010 Dec 16.

Infectious Diseases Unit, Vaccine Evaluation Centre, University Hospitals of Leicester NHS Trust and Department of Infection, Immunity, and Inflammation, University of Leicester, UK.

Background: Effective antigen-sparing vaccines are needed to confront pandemic influenza. Whole-virion and oil-in-water adjuvanted vaccines are the most effective formulations against H5N1 avian influenza. We assessed the safety and immunogenicity in adults in the UK of pandemic H1N1 whole-virion vaccine and oil-in-water adjuvanted vaccine purchased by the UK government in 2009. Read More

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http://dx.doi.org/10.1016/S1473-3099(10)70296-6DOI Listing
February 2011
36 Reads
1 Citation
22.430 Impact Factor

Head-to-head comparison of pandemic influenza vaccines.

Lancet Infect Dis 2011 Feb 16;11(2):74-5. Epub 2010 Dec 16.

Centre for Vaccinology, Ghent University and Hospital, Ghent, Belgium.

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http://dx.doi.org/10.1016/S1473-3099(10)70298-XDOI Listing
February 2011
10 Reads

Identification of an N-terminal 27 kDa fragment of Mycoplasma pneumoniae P116 protein as specific immunogen in M. pneumoniae infections.

BMC Infect Dis 2010 Dec 13;10:350. Epub 2010 Dec 13.

Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029, India.

Background: Mycoplasma pneumoniae is an important cause of respiratory tract infection and is increasingly being associated with other diseases such as asthma and extra-pulmonary complications. Considerable cross-reactivity is known to exist between the whole cell antigens used in the commercial serological testing assays. Identification of specific antigens is important to eliminate the risk of cross-reactions among different related organisms. Read More

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http://dx.doi.org/10.1186/1471-2334-10-350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022831PMC
December 2010
7 Reads