42 results match your criteria Comparative biochemistry and physiology. Part B Biochemistry & molecular biology[Journal]

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Hypoxia induces selective modifications to the acetylome in the brain of zebrafish (Danio rerio).

Comp Biochem Physiol B Biochem Mol Biol 2018 Oct 5;224:79-87. Epub 2018 Jan 5.

Department of Zoology, The University of British Columbia, 6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada.

Reversible protein acetylation is an important regulatory mechanism for modulating protein function. The cellular protein acetylome is in large part dictated by the cellular redox balance, and in particular [NAD]. While the relationship between hypoxia, redox balance, energy charge and resulting mitochondrial dysfunction has been examined in the context of hypoxia-linked pathologies, little is known about the direct effects of decreases in environmental oxygen on reversible lysine acetylation, and the resulting modifications to mitochondrial metabolism. Read More

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http://dx.doi.org/10.1016/j.cbpb.2017.12.018DOI Listing
October 2018
41 Reads

Metabolic suppression in the pelagic crab, Pleuroncodes planipes, in oxygen minimum zones.

Comp Biochem Physiol B Biochem Mol Biol 2018 Oct 27;224:88-97. Epub 2017 Dec 27.

Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada.

The pelagic red crab, Pleuroncodes planipes, is abundant throughout the Eastern Tropical Pacific in both benthic and pelagic environments to depths of several hundred meters. The oxygen minimum zones in this region reaches oxygen levels as low as 0.1kPa at depths within the crabs vertical range. Read More

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http://dx.doi.org/10.1016/j.cbpb.2017.12.017DOI Listing
October 2018
8 Reads
1.551 Impact Factor

Physiological and proteomic responses to corticosteroid treatments in Eurasian perch, Perca fluviatilis: Investigation of immune-related parameters.

Comp Biochem Physiol Part D Genomics Proteomics 2018 03 1;25:86-98. Epub 2017 Dec 1.

University of Namur, Research Unit in Environmental and Evolutionary Biology, rue de Bruxelles 61, B-5000 Namur, Belgium. Electronic address:

The comparative effects of cortisol and 11-deoxycorticosterone (DOC), two major corticosteroids in fish, have yet received little attention in teleosts. We evaluated the proteomic and immune responses of Eurasian perch to chronic corticosteroid treatments. We implanted immature perch with cortisol (80mg/kg) or DOC (4mg/kg) and measured the proportions of blood leucocytes, immune indices in the plasma, spleen and liver (complement and lysozyme activity, total immunoglobulin and immune gene expression in the tissues) and differential proteome expression (corticosteroid versus control) in the liver and the spleen on days 2, 4 and 14 post-treatment. Read More

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http://dx.doi.org/10.1016/j.cbd.2017.11.005DOI Listing
March 2018
44 Reads

Comparative biochemistry of cytochrome c oxidase in animals.

Comp Biochem Physiol B Biochem Mol Biol 2018 Oct 26;224:170-184. Epub 2017 Nov 26.

Queen's University, Kingston, Ontario, Canada.

Cytochrome c oxidase (COX), the terminal enzyme of the electron transport system, is central to aerobic metabolism of animals. Many aspects of its structure and function are highly conserved, yet, paradoxically, it is also an important model for studying the evolution of the metabolic phenotype. In this review, part of a special issue honouring Peter Hochachka, we consider the biology of COX from the perspective of comparative and evolutionary biochemistry. Read More

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http://dx.doi.org/10.1016/j.cbpb.2017.11.005DOI Listing
October 2018
13 Reads

Genome-wide identification of 52 cytochrome P450 (CYP) genes in the copepod Tigriopus japonicus and their B[α]P-induced expression patterns.

Comp Biochem Physiol Part D Genomics Proteomics 2017 09 6;23:49-57. Epub 2017 Jul 6.

Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea. Electronic address:

Cytochrome P450s (CYPs) are enzymes with a heme-binding domain that are found in all living organisms. CYP enzymes have important roles associated with detoxification of xenobiotics and endogenous compounds (e.g. Read More

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http://dx.doi.org/10.1016/j.cbd.2017.06.002DOI Listing
September 2017
3 Reads

Short-term starvation and realimentation helps stave off Edwardsiella tarda infection in red sea bream (Pagrus major).

Comp Biochem Physiol B Biochem Mol Biol 2017 Apr 2;206:42-53. Epub 2017 Feb 2.

Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukouka 812-8581, Japan.

Dietary regime modifications have been an integral part of health and healing practices throughout the animal kingdom. Thus, to assess the effects of periodic starvation and refeeding schedule on the physiological and immunological perturbations in Edwardsiella tarda infected red sea bream, we conducted a 20day experiment using 4 treatment groups, namely, pre-fed placebo (PFP); pre-starved placebo (PSP); pre-fed infected (PFI); and pre-starved infected (PSI), wherein a 5h E. tarda infection was done on the 11th day. Read More

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http://dx.doi.org/10.1016/j.cbpb.2017.01.009DOI Listing
April 2017
10 Reads

Activation of the Tor/Myc signaling axis in intestinal stem and progenitor cells affects longevity, stress resistance and metabolism in drosophila.

Comp Biochem Physiol B Biochem Mol Biol 2017 Jan 29;203:92-99. Epub 2016 Sep 29.

Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenka str., Ivano-Frankivsk 76018, Ukraine. Electronic address:

The TOR (target of rapamycin) signaling pathway and the transcriptional factor Myc play important roles in growth control. Myc acts, in part, as a downstream target of TOR to regulate the activity and functioning of stem cells. Here we explore the role of TOR-Myc axis in stem and progenitor cells in the regulation of lifespan, stress resistance and metabolism in Drosophila. Read More

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http://dx.doi.org/10.1016/j.cbpb.2016.09.008DOI Listing
January 2017
15 Reads
1 Citation
1.551 Impact Factor

De novo sequencing and transcriptome analysis of female venom glands of ectoparasitoid Bracon hebetor (Say.) (Hymenoptera: Braconidae).

Comp Biochem Physiol Part D Genomics Proteomics 2016 Dec 28;20:101-110. Epub 2016 Aug 28.

Department of Biochemistry, University of Agriculture Faisalabad, Pakistan.

Venom is a key-factor in the regulation of host physiology by parasitic Hymenoptera and a potentially rich source of novel bioactive substances for biotechnological applications. The limited study of venom from the ectoparasitoid Bracon hebetor, a tiny wasp that attacks larval pest insects of field and stored products and is thus a potential insect control agent, has not described the full complement and composition of these biomolecules. To have a comprehensive picture of genes expressed in the venom glands of B. Read More

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http://dx.doi.org/10.1016/j.cbd.2016.08.002DOI Listing
December 2016
8 Reads

Regulation of SMAD transcription factors during freezing in the freeze tolerant wood frog, Rana sylvatica.

Comp Biochem Physiol B Biochem Mol Biol 2016 Nov 15;201:64-71. Epub 2016 Jul 15.

Institute of Biochemistry and Department of Biology, Carleton University, Ottawa, ON, Canada. Electronic address:

The wood frog, Rana sylvatica, survives sub-zero winter temperatures by undergoing full body freezing for weeks at a time, during which it displays no measurable brain activity, no breathing, and a flat-lined heart. Freezing is a hypometabolic state characterized by a global suppression of gene expression that is elicited in part by transcription factors that coordinate the activation of vital pro-survival pathways. Smad transcription factors respond to TGF-β signalling and are involved in numerous cellular functions from development to stress. Read More

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http://dx.doi.org/10.1016/j.cbpb.2016.07.003DOI Listing
November 2016
12 Reads
1 Citation
1.551 Impact Factor

Proglucagons in vertebrates: Expression and processing of multiple genes in a bony fish.

Comp Biochem Physiol B Biochem Mol Biol 2016 Sep 27;199:58-66. Epub 2016 Feb 27.

Department of Biochemistry and Microbiology, and Department of Biology, University of Victoria, Victoria, BC, Canada.

In contrast to mammals, where a single proglucagon (PG) gene encodes three peptides: glucagon, glucagon-like peptide 1 and glucagon-like peptide 2 (GLP-1; GLP-2), many non-mammalian vertebrates carry multiple PG genes. Here, we investigate proglucagon mRNA sequences, their tissue expression and processing in a diploid bony fish. Copper rockfish (Sebastes caurinus) express two independent genes coding for distinct proglucagon sequences (PG I, PG II), with PG II lacking the GLP-2 sequence. Read More

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http://dx.doi.org/10.1016/j.cbpb.2016.02.004DOI Listing
September 2016
7 Reads

The duplicated paired box protein 7 (pax7) genes differentially transcribed during Japanese flounder (Paralichthys olivaceus) embryogenesis.

Comp Biochem Physiol B Biochem Mol Biol 2015 Nov 12;189:62-8. Epub 2015 Aug 12.

Key Laboratory of Experimental Marine Biology, National and Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, Shandong 266071, People's Republic of China.

PAX are important regulators of developmental processes. PAX7 plays crucial roles in patterning of the dorsal central nervous system (CNS), neural crest (NC), and skeletal muscle. Here, we identified six spliced isoforms of pax7a and one pax7b and characterized their expression patterns. Read More

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http://dx.doi.org/10.1016/j.cbpb.2015.08.003DOI Listing
November 2015
34 Reads

In-depth proteomic analysis of carp (Cyprinus carpio L) spermatozoa.

Comp Biochem Physiol Part D Genomics Proteomics 2014 Dec 28;12:10-5. Epub 2014 Sep 28.

Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Department of Gamete and Embryo Biology, Olsztyn, Poland.

Using a combination of protein fractionation by one-dimensional gel electrophoresis and high performance liquid chromatography-electrospray ionization tandem mass spectrometry, we identified 348 proteins in carp spermatozoa, most of which were for the first time identified in fish. Dynein, tubulin, HSP90, HSP70, HSP60, adenosylhomocysteinase, NKEF-B, brain type creatine kinase, mitochondrial ATP synthase, and valosin containing enzyme represent high abundance proteins in carp spermatozoa. These proteins are functionally related to sperm motility and energy production as well as the protection of sperm against oxidative injury and stress. Read More

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http://dx.doi.org/10.1016/j.cbd.2014.09.003DOI Listing
December 2014
4 Reads

Proteomic analysis of ovarian proteins and characterization of thymosin-β and RAC-GTPase activating protein 1 of the giant tiger shrimp Penaeus monodon.

Comp Biochem Physiol Part D Genomics Proteomics 2014 Sep 2;11:9-19. Epub 2014 Jun 2.

National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand; Center of Excellence for Marine Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address:

Cellular proteomics of total proteins in ovaries of domesticated and wild giant tiger shrimp (Penaeus monodon) were examined using GeLC-MS/MS. In total, 1638 proteins matched those previously deposited in databases and 1253 (76.50%) of these significantly matched known proteins. Read More

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http://dx.doi.org/10.1016/j.cbd.2014.05.002DOI Listing
September 2014
1 Read

Complete mitochondrial genomes of the Japanese pink coral (Corallium elatius) and the Mediterranean red coral (Corallium rubrum): a reevaluation of the phylogeny of the family Coralliidae based on molecular data.

Comp Biochem Physiol Part D Genomics Proteomics 2013 Sep 2;8(3):209-19. Epub 2013 Jun 2.

Laboratories of Biochemistry, Faculty of Science, Kochi University, Kochi 780-8520, Japan.

Precious corals are soft corals belonging to the family Coralliidae (Anthozoa: Octocorallia: Alcyonacea) and class Anthozoa, whose skeletal axes are used for jewelry. The family Coralliidae includes ca. 40 species and was originally thought to comprise of the single genus Corallium. Read More

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http://dx.doi.org/10.1016/j.cbd.2013.05.003DOI Listing
September 2013
16 Reads

Sex-specific expression, synthesis and localization of aromatase regulators in one-year-old Atlantic salmon ovaries and testes.

Comp Biochem Physiol B Biochem Mol Biol 2013 Apr 4;164(4):236-46. Epub 2013 Feb 4.

Department of Biology, Centre for Biomedical Research, University of Victoria, Victoria, British Columbia, Canada V8W 3N5.

Transcripts for dax1, foxl2, mis and sf1 are co-expressed in the somatic companion cells of teleost germ cells. These regulatory factors function, in part, to modulate the transcription of aromatase, particularly cyp19a, the terminal enzyme of estrogen biosynthesis. At least two separate aromatase loci exist in teleost fish that encode distinct isoforms. Read More

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http://dx.doi.org/10.1016/j.cbpb.2013.01.004DOI Listing
April 2013
11 Reads

Impact of toxicant exposure on the proteomic response to intertidal condition in Mytilus edulis.

Comp Biochem Physiol Part D Genomics Proteomics 2011 Dec 16;6(4):357-69. Epub 2011 Aug 16.

Laboratoire d'Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Faculté des Sciences et Techniques du Havre, 25 rue Philippe Lebon, 76058 Le Havre, France.

Intertidal blue mussels display physiological adaptations to emersion-submersion cycle that can be impacted by response to chemicals. In order to study the interference of cellular response to pollutants on intertidal physiology, we analysed proteomic (2-DE) responses in gills of mussels exposed for 14 days to regular emersion (intertidal condition) or continuous submersion (subtidal condition) and to a mixture (B[a]P/phenantrene) of polycyclic aromatic hydrocarbons (PAHs). Antioxidant activities were measured as general stress markers. Read More

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http://dx.doi.org/10.1016/j.cbd.2011.08.002DOI Listing
December 2011
2 Reads

Seasonal liver protein differences in a hibernator revealed by quantitative proteomics using whole animal isotopic labeling.

Comp Biochem Physiol Part D Genomics Proteomics 2011 Jun 5;6(2):163-70. Epub 2011 Mar 5.

Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, 80045, USA.

Hibernation is an energy-saving strategy used by diverse species of mammals to survive winter. It is characterized by cycles between multi-day periods of torpor with low body temperature (T(b)), and short periods of rapid, spontaneous rewarming. The ability to retain cellular integrity and function throughout torpor and rewarming is a key attribute of hibernation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S1744117X110000
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http://dx.doi.org/10.1016/j.cbd.2011.02.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183095PMC
June 2011
2 Reads

Comparative protein profiles: potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces).

Comp Biochem Physiol Part D Genomics Proteomics 2010 Dec;5(4):302-7

University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech Republic.

Sturgeon and paddlefish (Acipenseriformes), the source of roe consumed as caviar, are a unique and commercially valuable group of ancient fishes. In this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish: Siberian sturgeon (Acipenser baerii), sterlet (A. ruthenus), Russian sturgeon (A. Read More

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http://dx.doi.org/10.1016/j.cbd.2010.08.003DOI Listing
December 2010
6 Reads

Global analysis of circulating metabolites in hibernating ground squirrels.

Comp Biochem Physiol Part D Genomics Proteomics 2010 Dec 30;5(4):265-73. Epub 2010 Jul 30.

Department of Comparative Biosciences, University of Wisconsin School of Veterinary Medicine, Madison, WI 53706, USA.

Hibernation in mammals involves major alterations in nutrition and metabolism that would be expected to affect levels of circulating molecules. To gain insight into these changes we conducted a non-targeted LC-MS based metabolomic analysis of plasma using hibernating ground squirrels in late torpor (LT, T(b)~5 °C) or during an interbout arousal period (IBA, T(b)~5 °C) and non-hibernating squirrels in spring (T(b)~37 °C). Several metabolites varied and allowed differentiation between hibernators and spring squirrels, and between torpid and euthermic squirrels. Read More

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http://linkinghub.elsevier.com/retrieve/pii/S1744117X1000071
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http://dx.doi.org/10.1016/j.cbd.2010.07.002DOI Listing
December 2010
7 Reads

Differential protein expression profile in the liver of pikeperch (Sander lucioperca) larvae fed with increasing levels of phospholipids.

Comp Biochem Physiol Part D Genomics Proteomics 2010 Jun 2;5(2):130-7. Epub 2010 Apr 2.

Institut National des Sciences et Technologies de la Mer, Salammbo, Tunisia.

A comparative proteomic approach was used to assess the protein expression profile in the liver of 34days old pikeperch larvae fed from day 10 post hatching, with three isoproteic and isolipidic formulated diets varying by their phospholipid (PL) contents (% dry diet weight): 1.4% (PL1), 4.7% (PL5) and 9. Read More

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http://dx.doi.org/10.1016/j.cbd.2010.03.005DOI Listing
June 2010
6 Reads

Perspective--Exercise in fish: 50+years and going strong.

Authors:
James D Kieffer

Comp Biochem Physiol A Mol Integr Physiol 2010 Jun 16;156(2):163-8. Epub 2010 Feb 16.

Department of Biology & Canadian Rivers Institute, University of New Brunswick, Saint John, N.B. E2L 4L5, Canada.

Swimming in fish has garnered the attention of researchers for more than 50years. Research has focused on the mechanisms that fish utilize during exercise, fuel use patterns, recovery dynamics and the effects of abiotic and biotic factors on fish exercise physiology. Research direction has been influenced both by technology (e. Read More

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http://dx.doi.org/10.1016/j.cbpa.2010.02.009DOI Listing
June 2010
1 Read

Goat vitronectin: characterization and binding to Staphylococcus aureus.

Comp Biochem Physiol B Biochem Mol Biol 2008 Mar 17;149(3):410-8. Epub 2007 Nov 17.

Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, India.

Vitronectin (Vn) is a multifunctional protein present in plasma and in the extracellular matrix. Previous studies have demonstrated binding of many bacteria to human Vn. In this study, we have characterized goat Vn and studied its interaction with S. Read More

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http://dx.doi.org/10.1016/j.cbpb.2007.11.001DOI Listing
March 2008
7 Reads

The response of digestive proteases to abrupt salinity decrease in the euryhaline sparid Sparus aurata L.

Comp Biochem Physiol B Biochem Mol Biol 2007 Jun 3;147(2):156-63. Epub 2007 Mar 3.

Department of Biochemistry & Biotechnology, University of Thessaly, 26 Ploutonos Street, 41221 Larissa, Greece.

The response of the digestive proteases to abrupt salinity change was studied in juvenile gilthead sea bream (Sparus aurata) for 15 days after transfer from 33 per thousand to 21 per thousand. Salinity decrease affected significantly neither the activity of total acid proteases in stomach, nor the activities of total alkaline proteases and major serine proteases--trypsin and chymotrypsin--in the alkaline part of the intestine. The activity of the major proteases was significantly different between the alkaline segments of the intestine, with the posterior intestine presenting the highest activities followed by the pyloric caeca. Read More

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http://dx.doi.org/10.1016/j.cbpb.2006.12.021DOI Listing
June 2007
18 Reads

Use of suppression subtractive hybridization PCR for the development of cDNA arrays for the detection of endocrine disruption in carp (Cyprinus carpio).

Comp Biochem Physiol Part D Genomics Proteomics 2007 Mar 28;2(1):18-33. Epub 2006 Dec 28.

Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.

The potential of a variety of xenobiotic compounds to modulate or disrupt the endocrine system of humans and wildlife is now widely recognized. In the present study, we developed a molecular tool for the evaluation of endocrine disruption in common carp (Cyprinus carpio). Suppression Subtractive Hybridization PCR was applied for the isolation of a relevant gene set, consisting of gender- and hormone-responsive gene fragments. Read More

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http://dx.doi.org/10.1016/j.cbd.2006.10.004DOI Listing
March 2007
1 Read

Cloning and expression of insulin-like growth factors I and II in the shi drum (Umbrina cirrosa).

Comp Biochem Physiol B Biochem Mol Biol 2006 Jun 23;144(2):137-51. Epub 2006 Feb 23.

Department of Experimental Veterinary Sciences, Faculty of Veterinary Medicine, University of Padua, Agripolis, 35020 Legnaro (PD), Italy.

Insulin-like growth factors (IGFs) are evolutionarily ancient polypeptides, with potent metabolic actions, affecting cell development and growth. The IGF system consists of two ligands: IGF-I and IGF-II, several binding proteins and high-affinity transmembrane receptors. To understand growth regulation in the teleost shi drum, Umbrina cirrosa, we cloned IGF-I and IGF-II cDNAs, studied their expression and determined the cellular localization of IGF-II peptide by immunohistochemistry. Read More

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http://dx.doi.org/10.1016/j.cbpb.2006.02.003DOI Listing
June 2006
4 Reads

Isolation, characterization, and cDNA-derived amino acid sequence of glycocyamine kinase from the tropical marine worm Namalycastis sp.

Comp Biochem Physiol B Biochem Mol Biol 2005 Mar;140(3):387-93

Laboratory of Biochemistry, Faculty of Science, Kochi University, Kochi 780-8520, Japan.

We isolated cytoplasmic glycocyamine kinase (GK) and creatine kinase (CK) from the tropical marine worm Namalycastis sp. by ammonium sulfate fractionation, gel filtration on Sephacryl S-200, and DEAE-5PW chromatography. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed that the isolated GK is highly purified and appears to be a heterodimer of two distinct subunits, alpha and beta, with molecular masses of approximately 40 kDa. Read More

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http://dx.doi.org/10.1016/j.cbpc.2004.11.001DOI Listing
March 2005
4 Reads

Control of glycerol production by rainbow smelt (Osmerus mordax) to provide freeze resistance and allow foraging at low winter temperatures.

Comp Biochem Physiol B Biochem Mol Biol 2004 Nov;139(3):347-57

Ocean Sciences Centre, Memorial University of Newfoundland, Marine Lab. Drive, St. John's, NL, Canada A1C 5S7.

The rainbow smelt (Osmerus mordax) is a small anadromous fish that actively feeds under the ice at temperatures as low as the freeze point of seawater. Freezing is avoided through the production of both non-colligative antifreeze protein (AFP) and glycerol that acts in a colligative manner. Glycerol is constantly lost across the gills and skin, thus glycerol production must continue on a sustained basis at low winter temperatures. Read More

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http://dx.doi.org/10.1016/j.cbpc.2004.04.007DOI Listing
November 2004
1 Read

Sciatic nerve transection decrease substance P immunoreactivity in the lumbosacral spinal cord of the frog (Rana catesbeiana).

Comp Biochem Physiol B Biochem Mol Biol 2002 Apr;131(4):807-14

Departamento de Fisiologia, Laboratório de Neurobiologia Comparada, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170, RS, Porto Alegre, Brazil.

Using immunohistochemistry and optical densitometry, substance P (SP) was investigated in the lumbar spinal cord of the frog Rana catesbeiana after sciatic nerve transection. In control animals, there was a high density of SP fibers in the Lissauer's tract and in the mediolateral band of the dorsal gray matter. Other SP immunoreactive fibers were observed in the dorsal part of the lateral funiculus and in the ventral horn. Read More

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April 2002
2 Reads

Conserved cassette structure of vertebrate Mr 300 kDa mannose 6-phosphate receptors: partial cDNA sequence of fish MPR 300.

Comp Biochem Physiol B Biochem Mol Biol 2000 Dec;127(4):433-41

Department of Biochemistry, University of Hyderabad, India.

The existence of two homologous mannose 6-phosphate receptors (MPRs) with overlapping, but distinct functions has raised the question of at what stage in the phylogenetic tree the two receptors have occurred for the first time. In this paper, we present a partial cDNA sequence of Mr 300 kDa MPR (MPR 300) from poeciliid fish (Xiphophorus). It contains a 5'-untranslated region followed by the initiator ATG, and an open reading frame that corresponds to cassettes 1-5 and part of cassette 6 of mammalian MPR 300. Read More

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December 2000
3 Reads

Guinea pig alpha 1-microglobulin/bikunin: cDNA sequencing, tissue expression and expression during acute phase.

Comp Biochem Physiol B Biochem Mol Biol 1999 Feb;122(2):165-72

Department of Biochemistry, Kinki University School of Medicine, Osaka, Japan.

cDNA encoding alpha 1-microglobulin/bikunin (AMBP) was amplified from guinea pig (Cavia porcellus) liver mRNA by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods, cloned and sequenced. The deduced amino acid sequence was found to be homologous to the sequence of AMBP of other mammals (69-76% amino acid identity). It has two Kunitz-type trypsin inhibitor domains in the bikunin part as reactive sites, one in the N-terminal region and another in the C-terminal region. Read More

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February 1999
2 Reads

Antioxidant defenses and metabolic depression. The hypothesis of preparation for oxidative stress in land snails.

Comp Biochem Physiol B Biochem Mol Biol 1998 Jul;120(3):437-48

Departamento de Biologia Celular, Universidade de Brasilia, Brazil.

The roles of enzymatic antioxidant defenses in the natural tolerance of environmental stresses that impose changes in oxygen availability and oxygen consumption on animals is discussed with a particular focus on the biochemistry of estivation and metabolic depression in pulmonate land snails. Despite reduced oxygen consumption and PO2 during estivation, which should also mean reduced production of oxyradicals, the activities of antioxidant enzymes, such as superoxide dismutase and catalase, increased in 30 day-estivating snails. This appears to be an adaptation that allows the snails to deal with oxidative stress that takes place during arousal when PO2 and oxygen consumption rise rapidly. Read More

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July 1998
1 Read

Chick kidney ferredoxin: complementary DNA cloning and vitamin D effects on mRNA levels.

Comp Biochem Physiol B Biochem Mol Biol 1996 Aug;114(4):337-44

Biochemistry Department, University of California, Riverside 92521, USA.

Vertebrate ferredoxin is non-heme iron-sulfur protein found in steroideogenic tissues that serves as an electron shuttle in mitochondrial mixed function oxidase systems such as the 25-hydroxyvitamin D3-1 alpha-hydroxylase. A 2530-bp chick kidney ferredoxin cDNA was cloned, and the association between ferredoxin mRNA levels and the regulation of 1 alpha-hydroxylase activity by vitamin D status was examined. The cDNA sequence indicates that the chick kidney mitochondrial mixed function oxidases use the same ferredoxin as do those in the chick testis and that the chick ferredoxin shares greater than 92% amino acid homology with mammalian ferredoxins. Read More

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August 1996
1 Read

Carbohydrate content and monosaccharide composition of Rapana thomasiana grosse (Gastropoda) hemocyanin and its structural subunits. Comparison with gastropodan hemocyanins.

Comp Biochem Physiol B Biochem Mol Biol 1995 Apr;110(4):761-5

Department of Physical Biochemistry, University of Tübingen, Germany.

The hemocyanin of Rapana thomasiana grosse (marine snail, gastropod) is a glycoprotein with a carbohydrate content of 8.9% (w/w) and monosaccharide constituents xylose, fucose, 3-O-methylgalactose, mannose, galactose, N-acetylgalactosamine and N-acetylglucosamine residues. The two structural subunits of this oxygen carrier, RHSS1 and RHSS2, are unevenly glycosylated. Read More

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April 1995
1 Read

Insulin induction of c-Ki-ras in rat liver and in cultured normal rat hepatocytes.

Comp Biochem Physiol B 1993 Feb;104(2):341-7

Department of Biochemistry, University of Hong Kong.

1. Insulin administration in neonatal rats causes a dramatic accumulation of the major c-Ki-ras transcript. 2. Read More

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February 1993
1 Read

Proline transport by tsetse fly Glossina morsitans flight muscle mitochondria.

Comp Biochem Physiol B 1992 Jul;102(3):579-84

Department of Biochemistry, College of Health Sciences, University of Nairobi, Kenya, Africa.

1. Proline accumulation by tsetse fly Glossina morsitans flight muscle mitochondria was studied in vitro by the swelling technique and direct measurement of (U-14C) proline. 2. Read More

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July 1992
1 Read

Analysis of porcine gastric mucus glycoprotein added to a culture medium of Streptomyces sp. OH-11242 as the only source of carbon.

Comp Biochem Physiol B 1992 Apr;101(4):651-5

Department of Biochemistry, School of Medicine, Kitasato University, Kanagawa, Japan.

1. In the process of obtaining the degradation enzymes of mucus glycoprotein, porcine gastric mucus glycoprotein (PGM), added as the only source of carbon, was removed from the culture medium of Streptomyces sp. OH-11242 [Iwase et al. Read More

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April 1992
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Immunological analysis of chemoattractive proteins from earthworm to garter snakes.

Comp Biochem Physiol B 1992 Apr;101(4):601-10

Department of Biochemistry, SUNY Health Science Center, Brooklyn 11203.

1. Two sets of polyclonal antibodies to two highly purified prey-derived snake-attractive proteins, a low molecular weight (3000) protein and a 20,000 mol. wt protein, were generated in rabbits. Read More

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April 1992
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Fatty chain composition of phospholipids in sea urchin spermatozoa.

Authors:
M Mita N Ueta

Comp Biochem Physiol B 1989 ;92(2):319-22

Department of Biochemistry, Teikyo University School of Medicine, Tokyo, Japan.

1. An analysis was made of lipids extracted from the spermatozoa of the sea urchins, Hemicentrotus pulcherrimus and Anthocidaris crassispina. 2. Read More

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May 1989
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Comparative immunology of mammalian uricase using anti-hog liver uricase.

Comp Biochem Physiol B 1989 ;92(3):583-6

Department of Legal Medicine, Nagoya University School of Medicine, Japan.

1. Uricase was partially purified from livers of five mammals (dog, bovine, horse, house musk shrew and guinea pig), and its immunological properties were studied using anti-hog liver uricase prepared in rabbits. 2. Read More

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May 1989
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Protein composition of the threat induced epidermal secretion from the Arabian Gulf catfish, Arius thalassinus (Ruppell).

Comp Biochem Physiol B 1987 ;88(3):813-22

Department of Biochemistry, Faculty of Science, Kuwait University, Safat.

1. When threatened or injured, the Arabian Gulf catfish (Arius thalassinus, Ruppell) secretes a thick gel-like layer of proteinaceous material to its skin surface mainly from unicellular glands of the epidermis termed club cells. 2. Read More

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February 1988
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Factors affecting malate dehydrogenase activity in freezing-thawing processes.

Comp Biochem Physiol B 1987 ;88(2):461-6

Department of Biochemistry and Physiology, University of Barcelona, Spain.

Malate dehydrogenases from several sources show different behaviour when frozen-thawed in 100 mM sodium phosphate buffer, pH 7.4, containing chaotropic ions. The effects produced by the addition of various metabolites, protein concentration and buffer medium used on the loss of activity induced by the freezing-thawing process are reported. Read More

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March 1988
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Muscle structure and function in the goose, quail, pheasant, guinea hen, and chicken.

Authors:
K H Kiessling

Comp Biochem Physiol B 1977 ;57(4):287-92

Department of Animal Nutrition, Swedish University of Agriculture, Forestry and Veterinary Medicine, Uppsala.

1. Metabolic functions, fiber composition and, in some cases, mitochondrial morphology have been investigated in the pectoralis major and sartorius of the goose, quail, pheasant, guinea-hen, broiler chicken and laying hen. 2. Read More

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May 1989
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