148 results match your criteria Comparative Hepatology [Journal]


Systematic review of the diagnostic performance of serum markers of liver fibrosis in alcoholic liver disease.

Comp Hepatol 2012 Dec 28;11(1). Epub 2012 Dec 28.

Primary Care & Population Sciences Faculty of Medicine University of Southampton (MP 805) South Academic Block Southampton General Hospital, Tremona Rd, Southampton, SO16 6YD, UK.

Background: Alcoholic liver disease (ALD) is a significant cause of death and morbidity. Detection of liver fibrosis at an early stage could provide opportunities for more optimal management. Serum markers of liver fibrosis offer an alternative to biopsy. Read More

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http://comparative-hepatology.biomedcentral.com/articles/10.
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http://dx.doi.org/10.1186/1476-5926-11-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583674PMC
December 2012
11 Reads

N-acetylcysteine improves antitumoural response of Interferon alpha by NF-kB downregulation in liver cancer cells.

Comp Hepatol 2012 Dec 4;11(1). Epub 2012 Dec 4.

Post-Graduation Program in Medicine: Hepatology, Universidade Federal de Ciências da Saúde de Porto Alegre, Brazil, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, CEP: 90050-170, Brazil.

Unlabelled:

Background: Liver cancer is one of the most common malignancies in the world and at the moment, there is no drug intervention effective for the treatment of liver tumours. Investigate the effect of N-acetylcysteine (NAC), which has been studied for its antitumoural properties, on the toxicity of hepatocarcinoma (HCC) cells in vitro when used with the drug interferon alpha-2A (IFN), which is used clinically to treat HCC.

Results: NAC, IFN and NAC plus IFN reduced cell viability, as determined by MTT assay. Read More

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http://dx.doi.org/10.1186/1476-5926-11-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3539937PMC
December 2012
5 Reads

The genetic regulation of the terminating phase of liver regeneration.

Comp Hepatol 2012 Nov 20;11(1). Epub 2012 Nov 20.

Department of Digestive Surgery, University Hospital of Northern-Norway, Tromsø 9038, Norway.

Unlabelled:

Background: After partial hepatectomy (PHx), the liver regeneration process terminates when the normal liver-mass/body-weight ratio of 2.5% has been re-established. To investigate the genetic regulation of the terminating phase of liver regeneration, we performed a 60% PHx in a porcine model. Read More

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http://dx.doi.org/10.1186/1476-5926-11-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558440PMC
November 2012
7 Reads

Comparative histological study of hepatic architecture in the three orders amphibian livers.

Comp Hepatol 2012 Aug 20;11(1). Epub 2012 Aug 20.

Department of Biological Science, Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.

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Background: This report presents a detailed description of hepatic architecture in 46 amphibian livers by light microscopy, and extensively discusses the phylogenetic viewpoint.

Results: The 46 amphibian livers showed a variety of histological features, but anurans were the same as in mammalian livers. The hepatocyte-sinusoidal structures of the amphibian livers were classified into three different types: (I) several-cell-thick plate type, (II) two-cell-thick plate type, and (III) one-cell-thick plate type, depending on the percentage extension of sinusoidal areas per unit area, measured by morphometry. Read More

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http://dx.doi.org/10.1186/1476-5926-11-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517316PMC
August 2012
6 Reads

Severe diabetes and leptin resistance cause differential hepatic and renal transporter expression in mice.

Comp Hepatol 2012 Apr 23;11(1). Epub 2012 Apr 23.

Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI, 02881, USA.

Background: Type-2 Diabetes is a major health concern in the United States and other Westernized countries, with prevalence increasing yearly. There is a need to better model and predict adverse drug reactions, drug-induced liver injury, and drug efficacy in this population. Because transporters significantly contribute to drug clearance and disposition, it is highly significant to determine whether a severe diabetes phenotype alters drug transporter expression, and whether diabetic mouse models have altered disposition of acetaminophen (APAP) metabolites. Read More

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http://comparative-hepatology.biomedcentral.com/articles/10.
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http://dx.doi.org/10.1186/1476-5926-11-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416584PMC
April 2012
11 Reads

Protection against Fas-induced fulminant hepatic failure in liver specific integrin linked kinase knockout mice.

Comp Hepatol 2011 Nov 21;10:11. Epub 2011 Nov 21.

Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Background: Programmed cell death or apoptosis is an essential process for tissue homeostasis. Hepatocyte apoptosis is a common mechanism to many forms of liver disease. This study was undertaken to test the role of ILK in hepatocyte survival and response to injury using a Jo-2-induced apoptosis model. Read More

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http://comparative-hepatology.biomedcentral.com/articles/10.
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http://dx.doi.org/10.1186/1476-5926-10-11DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228663PMC
November 2011
7 Reads

The effect of cocoa supplementation on hepatic steatosis, reactive oxygen species and LFABP in a rat model of NASH.

Comp Hepatol 2011 Nov 14;10(1):10. Epub 2011 Nov 14.

School of Medicine, Deakin University, Waurn Ponds, Australia.

Background: Non alcoholic steatohepatitis is hypothesised to develop via a mechanism involving fat accumulation and oxidative stress. The current study aimed to investigate if an increase in oxidative stress was associated with changes in the expression of liver fatty acid binding protein in a rat model of non alcoholic steatohepatitis and whether cocoa supplementation attenuated those changes.

Methods: Female Sprague Dawley rats were fed a high fat control diet, a high fat methionine choline deficient diet, or one of four 12. Read More

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http://comparative-hepatology.biomedcentral.com/articles/10.
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http://dx.doi.org/10.1186/1476-5926-10-10DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3227569PMC
November 2011
9 Reads

Autoimmune liver disease - are there spectra that we do not know?

Comp Hepatol 2011 Sep 12;10. Epub 2011 Sep 12.

King Abdul Aziz University Hospital, Jeddah, Saudi Arabia.

Autoimmune liver diseases (AILDs) are common leading causes for liver cirrhosis and terminal stage of liver disease. They have variable prevalence among patients with liver disease and have two major clinical and biochemical presentations. Autoimmune hepatitis (AIH) is the typical example of hepatocellular AILD, but it can also be presented under a cholestatic pattern. Read More

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http://dx.doi.org/10.1186/1476-5926-10-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3179434PMC
September 2011
8 Reads
3 Citations

Evaluation of the effects of a VEGFR-2 inhibitor compound on alanine aminotransferase gene expression and enzymatic activity in the rat liver.

Comp Hepatol 2011 Aug 17;10. Epub 2011 Aug 17.

Drug Safety Research & Development, Pfizer Inc,, La Jolla, CA, Michigan State University, USA.

Background: Traditional assessment of drug-induced hepatotoxicity includes morphological examination of the liver and evaluation of liver enzyme activity in serum. The objective of the study was to determine the origin of drug-related elevation in serum alanine aminotransferase (ALT) activity in the absence of morphologic changes in the liver by utilizing molecular and immunohistochemical techniques.

Methods: Sixteen female Sprague-Dawley rats were divided into 2 groups (control and treated, n = 4 per group) and treated rats were dosed orally twice daily (400 mg/kg/day) for 7 days with a VEGFR-2 compound (AG28262), which in a previous study caused ALT elevation without morphological changes. Read More

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http://dx.doi.org/10.1186/1476-5926-10-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173283PMC
August 2011
11 Reads

Assessment and histological analysis of the IPRL technique for sequential in situ liver biopsy.

Comp Hepatol 2011 Aug 8;10. Epub 2011 Aug 8.

Faculty of Pharmacy, University of Sydney, NSW 2006, Australia.

Background: The isolated perfused rat liver (IPRL) is a technique used in a wide range of liver studies. Typically livers are assessed at treatment end point. Techniques have been described to biopsy liver in the live rat and post-hepatectomy. Read More

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http://dx.doi.org/10.1186/1476-5926-10-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199742PMC
August 2011
7 Reads

Situs inversus totalis and secondary biliary cirrhosis: a case report.

Comp Hepatol 2011 Aug 3;10. Epub 2011 Aug 3.

Ümraniye Education and Research Hospital, Department of Gastroenterology, Istanbul, Turkey.

Situs inversus totalis is is a congenital anomaly associated with various visceral abnormalities, but there is no data about the relationship between secondary biliary cirrhosis and that condition. We here present a case of a 58 year-old female with situs inversus totalis who was admitted to our clinic with extrahepatic cholestasis. After excluding all potential causes of biliary cirrhosis, secondary biliary cirrhosis was diagnosed based on the patient's history, imaging techniques, clinical and laboratory findings, besides histolopathological findings. Read More

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http://dx.doi.org/10.1186/1476-5926-10-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161837PMC
August 2011
9 Reads

Hepatic expression of multidrug resistance protein 2 in biliary atresia.

Comp Hepatol 2011 Aug 3;10. Epub 2011 Aug 3.

Department of Pediatric Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.

Background: Biliary atresia (BA) is an idiopathic inflammatory obliterative cholangiopathy of neonates, leading to progressive biliary cirrhosis. Hepatoportoenterostomy (Kasai procedure) can cure jaundice in 30% to 80% of patients. Postoperative clearance of jaundice is one of the most important factors influencing long-term outcomes of BA patients. Read More

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http://dx.doi.org/10.1186/1476-5926-10-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161838PMC
August 2011
7 Reads

Characterization of chronic HCV infection-induced apoptosis.

Comp Hepatol 2011 Jul 23;10(1). Epub 2011 Jul 23.

Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Egypt.

Background: To understand the complex and largely not well-understood apoptotic pathway and immune system evasion mechanisms in hepatitis C virus (HCV)-associated hepatocellular carcinoma (HCC) and HCV associated chronic hepatitis (CH), we studied the expression patterns of a number of pro-apoptotic and anti-apoptotic genes (Fas, FasL, Bcl-2, Bcl-xL and Bak) in HepG2 cell line harboring HCV- genotype-4 replication. For confirmation, we also assessed the expression levels of the same group of genes in clinical samples obtained from 35 HCC and 34 CH patients.

Methods: Viral replication was assessed in the tissue culture medium by RT-PCR, quantitative Real-Time PCR (qRT-PCR); detection of HCV core protein by western blot and inhibition of HCV replication with siRNA. Read More

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http://dx.doi.org/10.1186/1476-5926-10-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3160349PMC
July 2011
8 Reads

Effects of ischemic pre- and postconditioning on HIF-1α, VEGF and TGF-β expression after warm ischemia and reperfusion in the rat liver.

Comp Hepatol 2011 Jul 19;10(1). Epub 2011 Jul 19.

Department of Surgical Gastroenterology L, Aarhus University Hospital, Aarhus, Denmark.

Background: Ischemic pre- and postconditioning protects the liver against ischemia/reperfusion injuries. The aim of the present study was to examine how ischemic pre- and postconditioning affects gene expression of hypoxia inducible factor 1α (HIF-1α), vascular endothelial growth factor A (VEGF-A) and transforming growth factor β (TGF-β) in liver tissue.

Methods: 28 rats were randomized into five groups: control; ischemia/reperfusion; ischemic preconditioning (IPC); ischemic postconditioning (IPO); combined IPC and IPO. Read More

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http://comparative-hepatology.biomedcentral.com/articles/10.
Publisher Site
http://dx.doi.org/10.1186/1476-5926-10-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3155899PMC
July 2011
10 Reads

Characterization of Kupffer cells in livers of developing mice.

Comp Hepatol 2011 Jul 12;10(1). Epub 2011 Jul 12.

Department of Anatomy & Neurobiology, School of Medicine, University of California, Irvine CA, USA.

Background: Kupffer cells are well known macrophages of the liver, however, the developmental characteristics of Kupffer cells in mice are not well understood. To clarify this matter, the characteristics of Kupffer macrophages in normal developing mouse liver were studied using light microscopy and immunocytochemistry.

Methods: Sections of liver tissue from early postnatal mice were prepared using immunocytochemical techniques. Read More

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http://dx.doi.org/10.1186/1476-5926-10-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3148529PMC
July 2011
5 Reads

Hepatic oxidative stress in an animal model of sleep apnoea: effects of different duration of exposure.

Comp Hepatol 2011 Jul 5;10(1). Epub 2011 Jul 5.

Programa de Pós-Graduação em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Rio Grande do Sul, Brasil.

Background: Repeated apnoea events cause intermittent hypoxia (IH), which alters the function of various systems and produces free radicals and oxidative stress.

Methods: We investigated hepatic oxidative stress in adult mice subjected to intermittent hypoxia, simulating sleep apnoea. Three groups were submitted to 21 days of IH (IH-21), 35 days of IH (IH-35), or 35 days of sham IH. Read More

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http://dx.doi.org/10.1186/1476-5926-10-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144443PMC
July 2011
8 Reads

Expression of hepatocytic- and biliary-specific transcription factors in regenerating bile ducts during hepatocyte-to-biliary epithelial cell transdifferentiation.

Comp Hepatol 2010 Dec 2;9. Epub 2010 Dec 2.

Department of Pathology, School of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.

Background: Under compromised biliary regeneration, transdifferentiation of hepatocytes into biliary epithelial cells (BEC) has been previously observed in rats, upon exposure to BEC-specific toxicant methylene dianiline (DAPM) followed by bile duct ligation (BDL), and in patients with chronic biliary liver disease. However, mechanisms promoting such transdifferentiation are not fully understood. In the present study, acquisition of biliary specific transcription factors by hepatocytes leading to reprogramming of BEC-specific cellular profile was investigated as a potential mechanism of transdifferentiation in two different models of compromised biliary regeneration in rats. Read More

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http://dx.doi.org/10.1186/1476-5926-9-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014870PMC
December 2010
15 Reads

Response of sinusoidal mouse liver cells to choline-deficient ethionine-supplemented diet.

Comp Hepatol 2010 Oct 13;9. Epub 2010 Oct 13.

Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany.

Background: Proliferation of oval cells, the bipotent precursor cells of the liver, requires impeded proliferation and loss of hepatocytes as well as a specific micro-environment, provided by adjacent sinusoidal cells of liver. Despite their immense importance for triggering the oval cell response, cells of hepatic sinusoids are rarely investigated. To elucidate the response of sinusoidal liver cells we have employed a choline-deficient, ethionine-supplemented (CDE) diet, a common method for inducing an oval cell response in rodent liver. Read More

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http://dx.doi.org/10.1186/1476-5926-9-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964607PMC
October 2010
6 Reads

Adoptive transfer of splenocytes to study cell-mediated immune responses in hepatitis C infection using HCV transgenic mice.

Comp Hepatol 2010 Aug 20;9. Epub 2010 Aug 20.

Infectious Disease and Vaccine Research Centre, Children's Hospital of Eastern Ontario Research Institute, Ottawa, ON, Canada.

Background: Hepatitis C virus (HCV) is a major cause of chronic hepatitis and a health problem affecting over 170 million people around the world. We previously studied transgenic mice that express HCV Core, Envelope 1 and Envelope 2 proteins predominantly in the liver, resulting in steatosis, liver and lymphoid tumors, and hepatocellular carcinoma. Herein, the immune-mediated cell response to hepatitis C antigens was evaluated by adoptive transfers of carboxyfluorescein succinimidyl ester (CFSE) labelled splenocytes from HCV immunized mice into HCV transgenic mice. Read More

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http://dx.doi.org/10.1186/1476-5926-9-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2936292PMC
August 2010
19 Reads

An insertion mutation in ABCB4 is associated with gallbladder mucocele formation in dogs.

Comp Hepatol 2010 Jul 3;9. Epub 2010 Jul 3.

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-6610, USA.

Background: ABCB4 functions as a phosphatidylcholine translocater, flipping phosphatidylcholine across hepatocyte canalicular membranes into biliary canaliculi. In people, ABCB4 gene mutations are associated with several disease syndromes including intrahepatic cholestasis of pregnancy, progressive familial intrahepatic cholestasis (type 3), primary biliary cirrhosis, and cholelithiasis. Hepatobiliary disease, specifically gallbladder mucocele formation, has been recognized with increased frequency in dogs during the past decade. Read More

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http://dx.doi.org/10.1186/1476-5926-9-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2904718PMC
July 2010
11 Reads

Chlamydia trachomatis growth inhibition and restoration of LDL-receptor level in HepG2 cells treated with mevastatin.

Comp Hepatol 2010 Jan 28;9. Epub 2010 Jan 28.

Cambridge Theranostics Ltd, Babraham Research Campus, Babraham, Cambridge, CB2 4AT, UK.

Background: Perihepatitis is rare but consistently occurring extragenital manifestation of untreated Chlamydia trachomatis infection. Despite of possible liver involvement in generalized C. trachomatis infection, the ability of the pathogen to propagate in the hepatic cells and its impact on liver functions is not thoroughly investigated. Read More

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http://comparative-hepatology.biomedcentral.com/articles/10.
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http://dx.doi.org/10.1186/1476-5926-9-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2835644PMC
January 2010
12 Reads

Daytime food restriction alters liver glycogen, triacylglycerols, and cell size. A histochemical, morphometric, and ultrastructural study.

Comp Hepatol 2010 Feb 23;9. Epub 2010 Feb 23.

Instituto de Neurobiología, Campus UNAM-UAQ, Juriquilla, Querétaro, 76001 QRO, México.

Background: Temporal restriction of food availability entrains circadian behavioral and physiological rhythms in mammals by resetting peripheral oscillators. This entrainment underlies the activity of a timing system, different from the suprachiasmatic nuclei (SCN), known as the food entrainable oscillator (FEO). So far, the precise anatomical location of the FEO is unknown. Read More

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http://dx.doi.org/10.1186/1476-5926-9-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838809PMC
February 2010
6 Reads

Keratin 19 marks poor differentiation and a more aggressive behaviour in canine and human hepatocellular tumours.

Comp Hepatol 2010 Feb 18;9(1). Epub 2010 Feb 18.

Department of Clinical Sciences of Companion Animals, Faculty of Veterinary medicine, Utrecht University, Utrecht, The Netherlands.

Background: The expression of Keratin 19 (K19) was reported in a subset of hepatocellular carcinomas (HCCs). K19 positive HCCs are associated with an increased malignancy compared to K19 negative HCCs. No suitable mouse models exist for this subtype of HCC, nor is the incidence of K19 expression in hepatocellular neoplasia in model animals known. Read More

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http://dx.doi.org/10.1186/1476-5926-9-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2834617PMC
February 2010
9 Reads

Increased sinusoidal flow is not the primary stimulus to liver regeneration.

Comp Hepatol 2010 Jan 20;9. Epub 2010 Jan 20.

Surgical Research Laboratory, Institute of Clinical Medicine, University of Tromsoe, Tromsoe, Norway.

Background: Hemodynamic changes in the liver remnant following partial hepatectomy (PHx) have been suggested to be a primary stimulus in triggering liver regeneration. We hypothesized that it is the increased sinusoidal flow per se and hence the shear-stress stimulus on the endothelial surface within the liver remnant which is the main stimulus to regeneration. In order to test this hypothesis we wanted to increase the sinusoidal flow without performing a concomitant liver resection. Read More

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http://dx.doi.org/10.1186/1476-5926-9-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2819042PMC
January 2010
5 Reads

Serum levels of soluble Fas, soluble tumor necrosis factor-receptor II, interleukin-2 receptor and interleukin-8 as early predictors of hepatocellular carcinoma in Egyptian patients with hepatitis C virus genotype-4.

Comp Hepatol 2010 Jan 5;9(1). Epub 2010 Jan 5.

Background: Liver disease progression from chronic hepatitis C virus (HCV) infection to hepatocellular carcinoma (HCC) is associated with an imbalance between T-helper 1 and T-helper 2 cytokines. Evaluation of cytokines as possible candidate biomarkers for prediction of HCC was performed using soluble Fas(sFas), soluble tumor necrosis factor receptor-II (sTNFR-II), interleukin-2 receptor (IL-2R) and interleukin-8 (IL-8).

Results: The following patients were recruited: 79 with HCV infection, 30 with HCC, 32 with chronic liver disease associated with elevated liver enzyme levels (with or without cirrhosis) in addition to 17 with chronic HCV with persistent normal alanine aminotransferase levels (PNALT). Read More

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http://dx.doi.org/10.1186/1476-5926-9-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2819041PMC
January 2010
22 Reads

Immunohistochemical study of the phenotypic change of the mesenchymal cells during portal tract maturation in normal and fibrous (ductal plate malformation) fetal liver.

Comp Hepatol 2009 Jul 14;8. Epub 2009 Jul 14.

INSERM U889, Université Bordeaux2, F-33076 Bordeaux, France.

Background: In adult liver, the mesenchymal cells, portal fibroblasts and vascular smooth muscle cells can transdifferentiate into myofibroblasts, and are involved in portal fibrosis. Differential expression of markers, such as alpha-smooth muscle actin (ASMA), h-caldesmon and cellular retinol-binding protein-1 allows their phenotypic discrimination. The aim of our study was to explore the phenotypic evolution of the mesenchymal cells during fetal development in normal liver and in liver with portal fibrosis secondary to ductal plate malformation in a series of Meckel-Gruber syndrome, autosomal recessive polycystic kidney disease and Ivemark's syndrome. Read More

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http://dx.doi.org/10.1186/1476-5926-8-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2721154PMC
July 2009
5 Reads

Early effect of a single intravenous injection of ethanol on hepatic sinusoidal endothelial fenestrae in rabbits.

Comp Hepatol 2009 Jul 13;8. Epub 2009 Jul 13.

Center for Molecular and Vascular Biology, Department of Molecular and Cellular Medicine, University of Leuven, Herestraat 49, Leuven, 3000, Belgium.

Background: It has been postulated that ethanol affects hepatic sinusoidal and perisinusoidal cells. In the current experimental study, we investigated the early effect of a single intravenous dose of ethanol on the diameter of liver sinusoidal endothelial fenestrae in New Zealand White rabbits. The diameter of fenestrae in these rabbits is similar to the diameter found in humans with healthy livers. Read More

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http://comparative-hepatology.biomedcentral.com/articles/10.
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http://dx.doi.org/10.1186/1476-5926-8-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2715370PMC
July 2009
9 Reads

Comparison of different methods to obtain and store liver biopsies for molecular and histological research.

Comp Hepatol 2009 Jul 8;8. Epub 2009 Jul 8.

Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University Utrecht, Yalelaan 104, 3584 CM Utrecht, the Netherlands.

Background: To minimize the necessary number of biopsies for molecular and histological research we evaluated different sampling techniques, fixation methods, and storage procedures for canine liver tissue. For addressing the aim, three biopsy techniques (wedge biopsy, Menghini, True-cut), four storage methods for retrieval of RNA (snap freezing, RNAlater, Boonfix, RLT-buffer), two RNA isolation procedures (Trizol and RNAeasy), and three different fixation protocols for histological studies (10% buffered formalin, RNAlater, Boonfix) were compared. Histological evaluation was based on hematoxylin-eosin (HE) and reticulin (fibrogenesis) staining, and rubeanic acid and rhodanine stains for copper. Read More

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http://dx.doi.org/10.1186/1476-5926-8-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2717914PMC
July 2009
11 Reads

Bile constituents in hibernating golden-mantled ground squirrels (Spermophilus lateralis).

Comp Hepatol 2009 May 26;8. Epub 2009 May 26.

School of Life Sciences, University of Nevada Las Vegas, 4505 Maryland Parkway, Las Vegas Nevada 89154 USA.

Background: Golden-mantled ground squirrels (S. lateralis) are anorexic during the winter and survive by exploiting hibernation to reduce energetic demands. The liver normally plays a critical role in fueling and regulating metabolism and one might expect significant changes in hepatobiliary function with hibernation. Read More

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http://dx.doi.org/10.1186/1476-5926-8-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2692966PMC
May 2009
10 Reads

Low affinity glucocorticoid binding site ligands as potential anti-fibrogenics.

Comp Hepatol 2009 May 11;8. Epub 2009 May 11.

Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, UK.

Background: Pregnane X receptor (PXR) agonists inhibit liver fibrosis. However, the rodent PXR activator pregnenolone 16alpha carbonitrile (PCN) blocks, in vitro, hepatic stellate cell-to-myofibroblast trans-differentiation and proliferation in cells from mice with a disrupted PXR gene, suggesting there is an additional anti-fibrogenic drug target for PCN. The role of the low affinity glucocorticoid binding site (LAGS) - which may be identical or associated with the progesterone receptor membrane component 1 (PGRMC1) - in mediating this anti-fibrogenic effect has been examined, since binding of dexamethasone to the LAGS in liver microsomal membranes has previously been shown to be inhibited by PCN. Read More

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http://dx.doi.org/10.1186/1476-5926-8-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688476PMC
May 2009
8 Reads

Phospholipase C beta 4 in mouse hepatocytes: rhythmic expression and cellular distribution.

Comp Hepatol 2008 Oct 28;7. Epub 2008 Oct 28.

Department of Biology and Program in Neuroscience, College of Charleston, Charleston, SC 29424, USA.

Background: Circadian regulated physiological processes have been well documented in the mammalian liver. Phospholipases are important mediators of both cytoplasmic and nuclear signaling mechanisms in hepatocytes, and despite a potentially critical role for these enzymes in regulating the temporal aspect of hepatic physiology, their involvement in the circadian liver clock has not been the subject of much investigation. The phospholipase C beta4 (PLCbeta4) enzyme is of particular interest as it has been linked to circadian clock function. Read More

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http://dx.doi.org/10.1186/1476-5926-7-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2583973PMC
October 2008
6 Reads

Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications.

Comp Hepatol 2008 Oct 6;7. Epub 2008 Oct 6.

Duke University, Environmental Sciences and Policy Division, Nicholas School of the Environment and Earth Sciences, LSRC A333, Durham, NC, USA.

Background: A novel transparent stock of medaka (Oryzias latipes; STII), recessive for all pigments found in chromatophores, permits transcutaneous imaging of internal organs and tissues in living individuals. Findings presented describe the development of methodologies for non invasive in vivo investigation in STII medaka, and the successful application of these methodologies to in vivo study of hepatobiliary structure, function, and xenobiotic response, in both 2 and 3 dimensions.

Results: Using brightfield, and widefield and confocal fluorescence microscopy, coupled with the in vivo application of fluorescent probes, structural and functional features of the hepatobiliary system, and xenobiotic induced toxicity, were imaged at the cellular level, with high resolution (< 1 microm), in living individuals. Read More

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http://comparative-hepatology.biomedcentral.com/articles/10.
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http://dx.doi.org/10.1186/1476-5926-7-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2586619PMC
October 2008
7 Reads

A new parameter using serum lactate dehydrogenase and alanine aminotransferase level is useful for predicting the prognosis of patients at an early stage of acute liver injury: a retrospective study.

Comp Hepatol 2008 Aug 14;7. Epub 2008 Aug 14.

Department of Medicine and Bioregulatory Science, Graduate School of Medical Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.

Background: Although most patients with severe acute hepatitis are conservatively cured, some progress to acute liver failure (ALF) with a high rate of mortality. Based on the evidence that over-activation of macrophages, followed by disturbance of the hepatic microcirculation, plays a key role in ALF, we hypothesized that the production of serum lactate dehydrogenase (LDH) might increase in the liver under hypoxic conditions and could be an indicator to discriminate between conservative survivors and fatal patients at an early stage.

Results: To confirm this hypothesis, we developed a new parameter with serum alanine aminotransferase (ALT) and LDH: the ALT-LDH index = serum ALT/(serum LDH - median of normal LDH range). Read More

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http://comparative-hepatology.biomedcentral.com/articles/10.
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http://dx.doi.org/10.1186/1476-5926-7-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2527551PMC
August 2008
9 Reads

Relation between lipogranuloma formation and fibrosis, and the origin of brown pigments in lipogranuloma of the canine liver.

Comp Hepatol 2008 May 12;7. Epub 2008 May 12.

Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.

Background: In a previous study we confirmed that canine hepatic lipogranuloma, defined as lesions consisting of small round cells which contain lipid vacuoles and brown pigments in their cytoplasm, was an assembly of Kupffer cells and/or macrophages, and that the cytoplasmic brown pigments in the lesions were hemosiderin and ceroid. However, the pathogenesis of the lesion remains unclear. Kupffer cells (resident macrophages) play a key role in hepatic fibrogenesis due to the production of cytokines including TGF-beta. Read More

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http://comparative-hepatology.biomedcentral.com/articles/10.
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http://dx.doi.org/10.1186/1476-5926-7-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2391139PMC
May 2008
21 Reads

The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells.

Comp Hepatol 2008 May 5;7. Epub 2008 May 5.

Department of Cell Biology and Histology, IMB, Department of Medicine, IKM, Department of Orthopaedic Surgery, IKM, University of Tromsø, Norway.

Background: Liver sinusoidal endothelial cells (LSECs) are specialized scavenger cells, with crucial roles in maintaining hepatic and systemic homeostasis. Under normal physiological conditions, the oxygen tension encountered in the hepatic sinusoids is in general considerably lower than the oxygen tension in the air; therefore, cultivation of freshly isolated LSECs under more physiologic conditions with regard to oxygen would expect to improve cell survival, structure and function. In this study LSECs were isolated from rats and cultured under either 5% (normoxic) or 20% (hyperoxic) oxygen tensions, and several morpho-functional features were compared. Read More

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http://comparative-hepatology.biomedcentral.com/articles/10.
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http://dx.doi.org/10.1186/1476-5926-7-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2408922PMC
May 2008
8 Reads

Sustained virological and biochemical responses to lamivudine and adefovir dipivoxil combination in a chronic hepatitis B infection despite mutations conferring resistance to both drugs.

Comp Hepatol 2008 Mar 12;7. Epub 2008 Mar 12.

Laboratoire de Virologie moléculaire et structurale, CHU de Grenoble BP 217, 38043 Grenoble cedex 09, France.

Background: Sequential monotherapies of nucleotide analogs used in chronic hepatitis B treatment can lead to the selection of a resistance mutation to each antiviral drug.

Case Presentation: A patient with chronic hepatitis B was successively treated with lamivudine monotherapy, lamivudine-adefovir dual therapy, adefovir monotherapy and again with an adefovir-lamivudine dual therapy. Lamivudine-associated mutations (rtL180M and rtM204V/I) followed by adefovir-associated mutations (rtN236T and rtA181V) emerged during the two monotherapy regimens. Read More

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http://comparative-hepatology.biomedcentral.com/articles/10.
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http://dx.doi.org/10.1186/1476-5926-7-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2322943PMC
March 2008
26 Reads

Over-expression of glutamine synthase in focal nodular hyperplasia (part 1): early stages in the formation support the hypothesis of a focal hyper-arterialisation with venous (portal and hepatic) and biliary damage.

Comp Hepatol 2008 Feb 29;7. Epub 2008 Feb 29.

Service d'Anatomie Pathologique Hôpital Pellegrin, CHU Bordeaux, France.

Background: Most focal nodular hyperplasia (FNH) cases are diagnosed by chance. We studied a case of pre-FNH. We used glutamine synthase as an immunohistochemical marker for perivenous zones. Read More

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http://dx.doi.org/10.1186/1476-5926-7-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2292678PMC
February 2008
10 Reads

Plasma redox status is impaired in the portacaval shunted rat--the risk of the reduced antioxidant ability.

Comp Hepatol 2008 Feb 5;7. Epub 2008 Feb 5.

Surgery I Department, School of Medicine, Complutense University of Madrid, Spain.

Background: Portacaval shunting in rats produces a reduction of hepatic oxidant scavenging ability. Since this imbalance in hepatic oxidant/antioxidant homeostasis could coexist with systemic changes of oxidant stress/antioxidant status, plasma oxidants and antioxidant redox status in plasma of portacaval shunted-rats were determined.

Results: Male Wistar male: Control (n = 11) and with portacaval shunt (PCS; n = 11) were used. Read More

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http://comparative-hepatology.biomedcentral.com/articles/10.
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http://dx.doi.org/10.1186/1476-5926-7-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2262055PMC
February 2008
7 Reads

Establishment of an early liver fibrosis model by the hydrodynamics-based transfer of TGF-beta1 gene.

Comp Hepatol 2007 Oct 19;6. Epub 2007 Oct 19.

Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.

Background: Liver fibrosis represents a significant and severe health care problem and there are no efficient drugs for therapy so far. Preventing the progression of fibrogenesis and revival endogenous repair activities is an important strategy for both current and future therapies. Many studies of liver fibrosis consist of animal testing with various hepatotoxins. Read More

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http://dx.doi.org/10.1186/1476-5926-6-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2104536PMC
October 2007
5 Reads

Major HGF-mediated regenerative pathways are similarly affected in human and canine cirrhosis.

Comp Hepatol 2007 Jul 31;6. Epub 2007 Jul 31.

Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.

Background: The availability of non-rodent animal models for human cirrhosis is limited. We investigated whether privately-owned dogs (Canis familiaris) are potential model animals for liver disease focusing on regenerative pathways. Several forms of canine hepatitis were examined: Acute Hepatitis (AH), Chronic Hepatitis (CH), Lobular Dissecting Hepatitis (LDH, a specific form of micronodulair cirrhosis), and Cirrhosis (CIRR). Read More

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http://dx.doi.org/10.1186/1476-5926-6-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971050PMC
July 2007
5 Reads

Evolving concepts of liver fibrogenesis provide new diagnostic and therapeutic options.

Comp Hepatol 2007 Jul 30;6. Epub 2007 Jul 30.

Institute of Clinical Chemistry and Pathobiochemistry, RWTH-University Hospital, Aachen, Germany.

Despite intensive studies, the clinical opportunities for patients with fibrosing liver diseases have not improved. This will be changed by increasing knowledge of new pathogenetic mechanisms, which complement the "canonical principle" of fibrogenesis. The latter is based on the activation of hepatic stellate cells and their transdifferentiation to myofibroblasts induced by hepatocellular injury and consecutive inflammatory mediators such as TGF-beta. Read More

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http://dx.doi.org/10.1186/1476-5926-6-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1994681PMC
July 2007
6 Reads

Regeneration in pig livers by compensatory hyperplasia induces high levels of telomerase activity.

Comp Hepatol 2007 Jul 2;6. Epub 2007 Jul 2.

Department of Gastroenterology and Hepatology with Sections Infectious Disease and Tropical Medicine, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.

Background: Several highly proliferative human cells transiently activate telomerase, a ribonucleoprotein with reverse transcriptase activity, to counterbalance replication-associated telomere erosion and to increase stress resistance. Quiescent human hepatocytes exhibit very low or undetectable levels of telomerase activity. However, hepatocytes display a remarkable proliferative capability following liver injury. Read More

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http://dx.doi.org/10.1186/1476-5926-6-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1920532PMC
July 2007
9 Reads

Glucocorticoids activate TGF-beta induced PAI-1 and CTGF expression in rat hepatocytes.

Comp Hepatol 2007 May 2;6. Epub 2007 May 2.

Institute of Clinical Chemistry and Pathobiochemistry, University Hospital RWTH Aachen, Pauwelsstr, 30, D-52074 Aachen, Germany.

Background: In addition to the activation of hepatic stellate cells TGF-beta govern apoptosis and growth control of hepatocytes in liver injury. In non-parenchymal cells, TGF-beta induces plasminogen activator inhibitor 1 (PAI-1) and connective tissue growth factor (CTGF) expression, which are involved in extra cellular matrix formation. Both genes were also regulated by glucocorticoids, which in certain cases showed antagonistic effects to the TGF-beta-Smad 3 pathway. Read More

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http://dx.doi.org/10.1186/1476-5926-6-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1868771PMC
May 2007
9 Reads

CIDE-A is expressed in liver of old mice and in type 2 diabetic mouse liver exhibiting steatosis.

Comp Hepatol 2007 May 1;6. Epub 2007 May 1.

Edison Biotechnology Institute, Ohio University, Athens, OH 45701, USA.

Background: Increased levels of circulating fatty acids caused by insulin resistance and increased adipocyte lipolysis can accumulate within the liver resulting in steatosis. This steatosis sensitizes the liver to inflammation and further injury which can lead to liver dysfunction. We performed microarray analysis on normal mouse liver tissue at different ages and type 2 diabetic liver exhibiting steatosis to identify differentially expressed genes involved in lipid accumulation and liver dysfunction. Read More

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http://dx.doi.org/10.1186/1476-5926-6-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1868770PMC
May 2007
4 Reads

Effects of lamivudine on serum albumin levels correlate with pretreatment HBV-DNA levels in cirrhotic patients.

Comp Hepatol 2007 May 1;6. Epub 2007 May 1.

Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Japan.

Background: Lamivudine treatment has been recently demonstrated to increase the serum albumin levels in cirrhotic patients with hepatitis B virus (HBV) infection, but the precise mechanism remains unclear. We hypothesized that the improvement of hypoalbuminemia by lamivudine may be attributable to the reduction of HBV replication itself, rather than to cessation of hepatitis. In order to confirm this hypothesis, in this study we evaluated factors which correlated with the increase in serum albumin levels. Read More

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http://dx.doi.org/10.1186/1476-5926-6-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1884174PMC
May 2007
8 Reads

Interactions between estrogen- and Ah-receptor signalling pathways in primary culture of salmon hepatocytes exposed to nonylphenol and 3,3',4,4'-tetrachlorobiphenyl (congener 77).

Comp Hepatol 2007 Apr 13;6. Epub 2007 Apr 13.

Department of Biology, Norwegian University of science and Technology (NTNU), Høgskoleringen 5, 7491 Trondheim, Norway.

Background: The estrogenic and xenobiotic biotransformation gene expressions are receptor-mediated processes that are ligand structure-dependent interactions with estrogen-receptor (ER) and aryl hydrocarbon receptor (AhR), probably involving all subtypes and other co-factors. The anti-estrogenic activities of AhR agonists have been reported. In teleost fish, exposure to AhR agonists has been associated with reduced Vtg synthesis or impaired gonadal development in both in vivo- and in vitro studies. Read More

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http://dx.doi.org/10.1186/1476-5926-6-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1855068PMC
April 2007
7 Reads

TGF-beta dependent regulation of oxygen radicals during transdifferentiation of activated hepatic stellate cells to myofibroblastoid cells.

Comp Hepatol 2007 Feb 20;6. Epub 2007 Feb 20.

Department of Medicine I, Division Institute of Cancer Research, Medical University of Vienna, Borschke-Gasse 8a, A-1090 Vienna, Austria.

Background: The activation of hepatic stellate cells (HSCs) plays a pivotal role during liver injury because the resulting myofibroblasts (MFBs) are mainly responsible for connective tissue re-assembly. MFBs represent therefore cellular targets for anti-fibrotic therapy. In this study, we employed activated HSCs, termed M1-4HSCs, whose transdifferentiation to myofibroblastoid cells (named M-HTs) depends on transforming growth factor (TGF)-beta. Read More

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http://dx.doi.org/10.1186/1476-5926-6-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1804283PMC
February 2007
14 Reads

Comparison of tissue pressure and ablation time between the LeVeen and cool-tip needle methods.

Comp Hepatol 2006 Dec 21;5:10. Epub 2006 Dec 21.

Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Japan.

Background: Radio frequency ablation (RFA) has been accepted clinically as a useful local treatment for hepatocellular carcinoma (HCC). However, intrahepatic recurrence after RFA has been reported which might be attributable to increase in intra-tumor pressure during RFA. To reduce the pressure and ablation time, we developed a novel method of RFA, a multi-step method in which a LeVeen needle, an expansion-type electrode, is incrementally and stepwise expanded. Read More

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http://dx.doi.org/10.1186/1476-5926-5-10DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1764754PMC
December 2006
18 Reads

Sensitivity to endothelin-1 is decreased in isolated livers of endothelial constitutive nitric oxide synthase knockout mice.

Comp Hepatol 2006 Dec 5;5. Epub 2006 Dec 5.

Department of Clinical Pharmacology, University of Berne, Murtenstrasse 35, 3010 Berne, Switzerland.

Background: Hepatic sinusoidal resistance is regulated by vasoactive factors including endothelin-1 (ET-1) and nitric oxide (NO). In the absence of NO, vasoconstrictor response to endothelin is expected to predominate. Therefore, we hypothesized sensitivity to endothelin to be increased in mice lacking the endothelial cell NO synthase gene. Read More

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http://dx.doi.org/10.1186/1476-5926-5-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1697821PMC
December 2006
7 Reads

Feeding and fasting controls liver expression of a regulator of G protein signaling (Rgs16) in periportal hepatocytes.

Comp Hepatol 2006 Nov 23;5. Epub 2006 Nov 23.

Department of Pharmacology, UT Southwestern Medical Center, 6001 Forest Park Dr, Dallas, TX 75390-9041, USA.

Background: Heterotrimeric G protein signaling in liver helps maintain carbohydrate and lipid homeostasis. G protein signaling is activated by binding of extracellular ligands to G protein coupled receptors and inhibited inside cells by regulators of G protein signaling (RGS) proteins. RGS proteins are GTPase activating proteins, and thereby regulate Gi and/or Gq class G proteins. Read More

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http://dx.doi.org/10.1186/1476-5926-5-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1687201PMC
November 2006
8 Reads