124 results match your criteria Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology [Journal]


A molecular survey of programmed cell death in rainbow trout: Structural and functional specifications of apoptotic key molecules.

Comp Biochem Physiol B Biochem Mol Biol 2019 Apr 28;230:57-69. Epub 2019 Jan 28.

Fish Genetics Unit, Institute of Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany. Electronic address:

Apoptosis is an integral part of homeostasis and supports multiple physiological processes such as development and immune defense, thereby directly targeting damaged or unwanted cells without affecting neighbor cells. In the present study, we characterized the apoptotic key factors caspase-3, -7, and - 8 as well as regulator protein TPT1 (translationally-controlled tumor protein 1) from rainbow trout (Oncorhynchus mykiss). We identified multiple single-nucleotide changes in their coding sequences and showed that the CASP3 gene is present in at least three variants. Read More

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http://dx.doi.org/10.1016/j.cbpb.2019.01.012DOI Listing

Hypoxia induces selective modifications to the acetylome in the brain of zebrafish (Danio rerio).

Comp Biochem Physiol B Biochem Mol Biol 2018 Oct 5;224:79-87. Epub 2018 Jan 5.

Department of Zoology, The University of British Columbia, 6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada.

Reversible protein acetylation is an important regulatory mechanism for modulating protein function. The cellular protein acetylome is in large part dictated by the cellular redox balance, and in particular [NAD]. While the relationship between hypoxia, redox balance, energy charge and resulting mitochondrial dysfunction has been examined in the context of hypoxia-linked pathologies, little is known about the direct effects of decreases in environmental oxygen on reversible lysine acetylation, and the resulting modifications to mitochondrial metabolism. Read More

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http://dx.doi.org/10.1016/j.cbpb.2017.12.018DOI Listing
October 2018
41 Reads

Metabolic suppression in the pelagic crab, Pleuroncodes planipes, in oxygen minimum zones.

Comp Biochem Physiol B Biochem Mol Biol 2018 Oct 27;224:88-97. Epub 2017 Dec 27.

Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada.

The pelagic red crab, Pleuroncodes planipes, is abundant throughout the Eastern Tropical Pacific in both benthic and pelagic environments to depths of several hundred meters. The oxygen minimum zones in this region reaches oxygen levels as low as 0.1kPa at depths within the crabs vertical range. Read More

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http://dx.doi.org/10.1016/j.cbpb.2017.12.017DOI Listing
October 2018
8 Reads
1.551 Impact Factor

The response of glutathione peroxidase 1 and glutathione peroxidase 7 under different oxidative stresses in black tiger shrimp, Penaeus monodon.

Comp Biochem Physiol B Biochem Mol Biol 2018 Mar 12;217:1-13. Epub 2017 Dec 12.

Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, PR China; Key Laboratory of Fishery Ecology and Environment, Guangdong Province, PR China. Electronic address:

Glutathione peroxidase (GPx) is part of the enzymatic antioxidant system that can eliminate the peroxides produced as effect of reactions of molecules with reactive oxygen species (ROS). We cloned two different GPx genes from Penaeus monodon (black tiger shrimp). Bioinformatics sequence analysis showed that PmGPx1 and PmGPx7 encoded polypeptides of 207 and 208 amino acids, respectively. Read More

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http://dx.doi.org/10.1016/j.cbpb.2017.12.009DOI Listing
March 2018
11 Reads

Comparative biochemistry of cytochrome c oxidase in animals.

Comp Biochem Physiol B Biochem Mol Biol 2018 Oct 26;224:170-184. Epub 2017 Nov 26.

Queen's University, Kingston, Ontario, Canada.

Cytochrome c oxidase (COX), the terminal enzyme of the electron transport system, is central to aerobic metabolism of animals. Many aspects of its structure and function are highly conserved, yet, paradoxically, it is also an important model for studying the evolution of the metabolic phenotype. In this review, part of a special issue honouring Peter Hochachka, we consider the biology of COX from the perspective of comparative and evolutionary biochemistry. Read More

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http://dx.doi.org/10.1016/j.cbpb.2017.11.005DOI Listing
October 2018
13 Reads

Short-term starvation and realimentation helps stave off Edwardsiella tarda infection in red sea bream (Pagrus major).

Comp Biochem Physiol B Biochem Mol Biol 2017 Apr 2;206:42-53. Epub 2017 Feb 2.

Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukouka 812-8581, Japan.

Dietary regime modifications have been an integral part of health and healing practices throughout the animal kingdom. Thus, to assess the effects of periodic starvation and refeeding schedule on the physiological and immunological perturbations in Edwardsiella tarda infected red sea bream, we conducted a 20day experiment using 4 treatment groups, namely, pre-fed placebo (PFP); pre-starved placebo (PSP); pre-fed infected (PFI); and pre-starved infected (PSI), wherein a 5h E. tarda infection was done on the 11th day. Read More

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http://dx.doi.org/10.1016/j.cbpb.2017.01.009DOI Listing
April 2017
10 Reads

Activation of the Tor/Myc signaling axis in intestinal stem and progenitor cells affects longevity, stress resistance and metabolism in drosophila.

Comp Biochem Physiol B Biochem Mol Biol 2017 Jan 29;203:92-99. Epub 2016 Sep 29.

Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenka str., Ivano-Frankivsk 76018, Ukraine. Electronic address:

The TOR (target of rapamycin) signaling pathway and the transcriptional factor Myc play important roles in growth control. Myc acts, in part, as a downstream target of TOR to regulate the activity and functioning of stem cells. Here we explore the role of TOR-Myc axis in stem and progenitor cells in the regulation of lifespan, stress resistance and metabolism in Drosophila. Read More

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http://dx.doi.org/10.1016/j.cbpb.2016.09.008DOI Listing
January 2017
15 Reads
1 Citation
1.551 Impact Factor

Effects of fasting and refeeding on gene expression of slc15a1a, a gene encoding an oligopeptide transporter (PepT1), in the intestine of Mozambique tilapia.

Comp Biochem Physiol B Biochem Mol Biol 2017 Jan 30;203:76-83. Epub 2016 Sep 30.

Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo 113-8657, Japan. Electronic address:

The tissue distribution of slc15a1a, a gene that encodes an oligopeptide transporter, PepT1, and its response to fasting and refeeding were investigated in the intestinal epithelium of Mozambique tilapia for a better understanding of its role on nutrient absorption. The slc15a1a was predominantly expressed in the absorptive epithelia of the anterior part of the intestine, suggesting that digested oligopeptides are primarily absorbed in the anterior intestine. The response of slc15a1a to fasting was evaluated at 1, 2, 4, 7 and 14days after the last feeding. Read More

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http://dx.doi.org/10.1016/j.cbpb.2016.09.006DOI Listing
January 2017
19 Reads

Regulation of SMAD transcription factors during freezing in the freeze tolerant wood frog, Rana sylvatica.

Comp Biochem Physiol B Biochem Mol Biol 2016 Nov 15;201:64-71. Epub 2016 Jul 15.

Institute of Biochemistry and Department of Biology, Carleton University, Ottawa, ON, Canada. Electronic address:

The wood frog, Rana sylvatica, survives sub-zero winter temperatures by undergoing full body freezing for weeks at a time, during which it displays no measurable brain activity, no breathing, and a flat-lined heart. Freezing is a hypometabolic state characterized by a global suppression of gene expression that is elicited in part by transcription factors that coordinate the activation of vital pro-survival pathways. Smad transcription factors respond to TGF-β signalling and are involved in numerous cellular functions from development to stress. Read More

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http://dx.doi.org/10.1016/j.cbpb.2016.07.003DOI Listing
November 2016
12 Reads
1 Citation
1.551 Impact Factor

Proglucagons in vertebrates: Expression and processing of multiple genes in a bony fish.

Comp Biochem Physiol B Biochem Mol Biol 2016 Sep 27;199:58-66. Epub 2016 Feb 27.

Department of Biochemistry and Microbiology, and Department of Biology, University of Victoria, Victoria, BC, Canada.

In contrast to mammals, where a single proglucagon (PG) gene encodes three peptides: glucagon, glucagon-like peptide 1 and glucagon-like peptide 2 (GLP-1; GLP-2), many non-mammalian vertebrates carry multiple PG genes. Here, we investigate proglucagon mRNA sequences, their tissue expression and processing in a diploid bony fish. Copper rockfish (Sebastes caurinus) express two independent genes coding for distinct proglucagon sequences (PG I, PG II), with PG II lacking the GLP-2 sequence. Read More

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http://dx.doi.org/10.1016/j.cbpb.2016.02.004DOI Listing
September 2016
7 Reads

Myostatin inhibitory region of fish (Paralichthys olivaceus) myostatin-1 propeptide.

Comp Biochem Physiol B Biochem Mol Biol 2016 Apr-May;194-195:65-70. Epub 2016 Jan 28.

Department of Human Nutrition, Food and Animal Sciences, University of Hawaii, 1955 East-West Rd., Honolulu, HI 96822, USA. Electronic address:

Myostatin (MSTN) is a potent negative regulator of skeletal muscle growth, and its activity is suppressed by MSTN propeptide (MSTNpro), the N-terminal part of MSTN precursor cleaved during post-translational MSTN processing. The current study examined which region of flatfish (Paralichthys olivaceus) MSTN-1 propeptide (MSTN1pro) is critical for MSTN inhibition. Six different truncated forms of MSTN1pro containing N-terminal maltose binding protein (MBP) as a fusion partner were expressed in Escherichia coli, and partially purified by an affinity chromatography for MSTN-inhibitory activity examination. Read More

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http://dx.doi.org/10.1016/j.cbpb.2016.01.010DOI Listing
December 2016
15 Reads

The potential role of polyamines in gill epithelial remodeling during extreme hypoosmotic challenges in the Gulf killifish, Fundulus grandis.

Comp Biochem Physiol B Biochem Mol Biol 2016 Apr-May;194-195:39-50. Epub 2016 Jan 11.

Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.

Polyamines are a family of low molecular weight organic cations produced in part by the coordinated actions of arginase II (Arg II) and ornithine decarboxylase (Odc). Although gill polyamine homeostasis is affected by acute transfer to fresh water, little is known of its function in fish osmoregulation. The current study investigated the role of polyamines in the compensatory response of hypoosmotic challenge in the euryhaline fish, Fundulus grandis. Read More

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http://dx.doi.org/10.1016/j.cbpb.2016.01.003DOI Listing
December 2016
19 Reads

Coloration reflects skin pterin concentration in a red-tailed lizard.

Comp Biochem Physiol B Biochem Mol Biol 2016 Mar 3;193:17-24. Epub 2015 Dec 3.

Departamento de Ecología Evolutiva, Estación Biológica de Doñana (CSIC), Calle Américo Vespucio s/n, 41092 Sevilla, Spain. Electronic address:

When integumentary tissue pigments are contained in chromatophores, tissue color might not depend exclusively on the amount of pigment. Whether coloration does or does not reflect pigment concentration may be very significant for intraspecific communication, for example when pigment concentration provides fitness-related information. We studied the pigment responsible for the orange/red ventral tail coloring in a lacertid lizard species (Acanthodactylus erythrurus), and whether the color was related to skin pigment concentration. Read More

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http://dx.doi.org/10.1016/j.cbpb.2015.11.011DOI Listing
March 2016
20 Reads

Mitochondrial reactive oxygen species production by fish muscle mitochondria: Potential role in acute heat-induced oxidative stress.

Comp Biochem Physiol B Biochem Mol Biol 2016 Jan 9;191:99-107. Epub 2015 Oct 9.

Department of Biological Sciences, University of Manitoba, Winnipeg, MB, Canada; Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, Canada. Electronic address:

Acute heat challenge is known to induce cell-level oxidative stress in fishes. Mitochondria are well known for the capacity to make reactive oxygen species (ROS) and as such are often implicated as a source of the oxidants associated with this thermally-induced oxidative stress. This implication is often asserted, despite little direct data for mitochondrial ROS metabolism in fishes. Read More

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http://dx.doi.org/10.1016/j.cbpb.2015.10.001DOI Listing
January 2016
10 Reads

The duplicated paired box protein 7 (pax7) genes differentially transcribed during Japanese flounder (Paralichthys olivaceus) embryogenesis.

Comp Biochem Physiol B Biochem Mol Biol 2015 Nov 12;189:62-8. Epub 2015 Aug 12.

Key Laboratory of Experimental Marine Biology, National and Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, Shandong 266071, People's Republic of China.

PAX are important regulators of developmental processes. PAX7 plays crucial roles in patterning of the dorsal central nervous system (CNS), neural crest (NC), and skeletal muscle. Here, we identified six spliced isoforms of pax7a and one pax7b and characterized their expression patterns. Read More

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http://dx.doi.org/10.1016/j.cbpb.2015.08.003DOI Listing
November 2015
34 Reads

cfMSP-1, an extremely acidic matrix protein involved in shell formation of the scallop Chlamys farreri.

Comp Biochem Physiol B Biochem Mol Biol 2015 Jul 28;185:34-41. Epub 2015 Mar 28.

Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Protein Science Laboratory of the Ministry of Education, Tsinghua University, Beijing 100084, China. Electronic address:

Matrix proteins play an important role in biomineralization by mollusks. In this study, we cloned and characterized an acidic protein (pI=3.36) homolog of cfMSP-1 that is highly expressed in the mantle transcriptome of the scallop Chlamys farreri. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10964959150004
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http://dx.doi.org/10.1016/j.cbpb.2015.03.004DOI Listing
July 2015
27 Reads

RNA interference mediated knockdown of the KDEL receptor and COPB2 inhibits digestion and reproduction in the parasitic copepod Lepeophtheirus salmonis.

Comp Biochem Physiol B Biochem Mol Biol 2014 Apr 29;170:1-9. Epub 2013 Dec 29.

SLRC-Sea Lice Research Centre, Institute of Marine Research, P.O. Box 1870, 5817 Bergen, Norway.

Retrograde transport of proteins from the endoplasmic reticulum to the Golgi is an essential part of the secretory pathway that all newly synthesised secreted and membrane proteins in eukaryotic cells undergo. The aim of this study was to characterise two components of the retrograde transport pathway in the parasitic copepod Lepeophtheirus salmonis (salmon louse) on a molecular and functional level. LsKDELR and LsCOPB2 were confirmed to be the salmon louse homologues of the chosen target proteins by sequence similarity. Read More

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http://dx.doi.org/10.1016/j.cbpb.2013.12.006DOI Listing
April 2014
9 Reads

Acute endocrine and nutritional co-regulation of the hepatic omy-miRNA-122b and the lipogenic gene fas in rainbow trout, Oncorhynchus mykiss.

Comp Biochem Physiol B Biochem Mol Biol 2014 Mar 11;169:16-24. Epub 2013 Dec 11.

INRA, UR 1067, Nutrition, Métabolisme, Aquaculture, Aquapôle, F-64310 Saint-Pée-sur-Nivelle, France. Electronic address:

Hepatic lipogenesis represents a crucial part of intermediary metabolism and is acutely regulated by endocrine factors and nutrients. The liver-specific and highly abundant microRNA-122 has emerged as a powerful regulator of lipogenesis in higher vertebrates, but little is known about its endocrine and nutritional regulation. In this study, we investigated the hypothesis that insulin regulates hepatic expression of omy-miRNA-122 isomiRNAs (omy-miRNA-122a and omy-miRNA-122b) by using in vivo and in vitro approaches. Read More

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http://dx.doi.org/10.1016/j.cbpb.2013.12.002DOI Listing
March 2014
12 Reads
1.551 Impact Factor

Molecular diversity and body distribution of saponins in the sea star Asterias rubens by mass spectrometry.

Comp Biochem Physiol B Biochem Mol Biol 2014 Feb 29;168:1-11. Epub 2013 Oct 29.

Mass Spectrometry Research Group, Interdisciplinary Center for Mass Spectrometry, University of Mons - UMONS, 23 Place du Parc, B-7000 Mons, Belgium. Electronic address:

Saponins are natural molecules that the common sea star Asterias rubens produces in the form of steroid glycosides bearing a sulfate group attached on the aglycone part. In order to highlight the inter-organ and inter-individual variability, the saponin contents of five distinct body components, namely the aboral body wall, the oral body wall, the stomach, the pyloric caeca and the gonads, from different individuals were separately analyzed by mass spectrometry. MALDI-ToF experiments were selected as the primary tool for a rapid screening of the saponin mixtures, whereas LC-MS and LC-MS/MS techniques were used to achieve chromatographic separation of isomers. Read More

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http://www.vliz.be/imisdocs/publications/279430.pdf
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http://linkinghub.elsevier.com/retrieve/pii/S109649591300176
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http://dx.doi.org/10.1016/j.cbpb.2013.10.004DOI Listing
February 2014
14 Reads

Chemical fingerprinting and phylogenetic mapping of saponin congeners from three tropical holothurian sea cucumbers.

Comp Biochem Physiol B Biochem Mol Biol 2013 Nov-Dec;166(3-4):182-93. Epub 2013 Sep 12.

The Marine Science Institute, University of the Philippines, Diliman, Quezon City, Philippines. Electronic address:

Holothurians are sedentary marine organisms known to produce saponins (triterpene glycosides), secondary metabolites exhibiting a wide range of biological activities. In this paper, we investigated the saponin contents of semi-purified and membranolytic HPLC fractionated extracts from the body wall of three species of Holothuriidae as an attempt to examine its chemical diversity in relation to phylogenetic data. MALDI-FTICR MS and nano-HPLC-chip Q-TOF MS were used for mass profiling and isomer separation, respectively giving a unique chemical saponin fingerprint. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10964959130014
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http://dx.doi.org/10.1016/j.cbpb.2013.09.002DOI Listing
June 2014
10 Reads

Molecular cloning and expression analysis of two β-defensin genes in the blunt snout bream (Megalobrama amblycephala).

Comp Biochem Physiol B Biochem Mol Biol 2013 Sep 20;166(1):91-8. Epub 2013 Jul 20.

Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China.

β-Defensins are a group of cysteine-rich, cationic antimicrobial peptides that play important roles in innate immune system against pathogenic microbes invading. In this study, the part-length cDNA sequences of two β-defensin genes (maΒD-1, maΒD-2) in blunt snout bream (Megalobrama amblycephala) were identified. Homology analysis showed that the cDNA sequences of maΒD-1 and maΒD-2 had high similarities to those in common carp and zebrafish. Read More

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http://dx.doi.org/10.1016/j.cbpb.2013.07.006DOI Listing
September 2013
13 Reads
2 Citations
1.550 Impact Factor

Characterization of the expression profiles of adipogenesis-related factors, ZNF423, KLFs and FGF10, during preadipocyte differentiation and abdominal adipose tissue development in chickens.

Comp Biochem Physiol B Biochem Mol Biol 2013 Jul 19;165(3):189-95. Epub 2013 Apr 19.

Animal Science, Department of Biological Production, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo, 183-8509, Japan.

Adipogenesis is controlled by a complicated process involving certain transcriptional events. In chicken adipogenesis, peroxisome proliferator-activated receptor γ (PPARγ) is a key regulator of preadipocyte differentiation and abdominal fat accumulation. However, in a recent study in mammals, some novel factors related to regulation of adipogenesis, including preadipocyte differentiation, were identified in mammals. Read More

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http://dx.doi.org/10.1016/j.cbpb.2013.04.002DOI Listing
July 2013
10 Reads

Sex-specific expression, synthesis and localization of aromatase regulators in one-year-old Atlantic salmon ovaries and testes.

Comp Biochem Physiol B Biochem Mol Biol 2013 Apr 4;164(4):236-46. Epub 2013 Feb 4.

Department of Biology, Centre for Biomedical Research, University of Victoria, Victoria, British Columbia, Canada V8W 3N5.

Transcripts for dax1, foxl2, mis and sf1 are co-expressed in the somatic companion cells of teleost germ cells. These regulatory factors function, in part, to modulate the transcription of aromatase, particularly cyp19a, the terminal enzyme of estrogen biosynthesis. At least two separate aromatase loci exist in teleost fish that encode distinct isoforms. Read More

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http://dx.doi.org/10.1016/j.cbpb.2013.01.004DOI Listing
April 2013
11 Reads

Genetic ontogeny of pancreatic enzymes in Labrus bergylta larvae and the effect of feed type on enzyme activities and gene expression.

Comp Biochem Physiol B Biochem Mol Biol 2013 Mar 20;164(3):176-84. Epub 2012 Dec 20.

National Institute of Nutrition and Seafood Research, PO Box 2029, Nordnes, 5817 Bergen, Norway.

A newly cultivated wrasse species, Labrus bergylta, have shown great potential for use in Atlantic salmon (Salmo salar) farms in the battle against sea lice (Lepeoptheirus salmonis) infections. Hatchery reared L. bergylta were studied from 2 to 55 DPH to examine the molecular basis of digestive ontogeny related to the pancreas. Read More

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http://dx.doi.org/10.1016/j.cbpb.2012.12.001DOI Listing
March 2013
14 Reads

White shrimp Litopenaeus vannamei catalase: gene structure, expression and activity under hypoxia and reoxygenation.

Comp Biochem Physiol B Biochem Mol Biol 2013 Jan 8;164(1):44-52. Epub 2012 Nov 8.

Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo, Sonora, Mexico.

Catalase (EC 1.11.1. Read More

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http://dx.doi.org/10.1016/j.cbpb.2012.10.004DOI Listing
January 2013
9 Reads

Effects of recombinant gonadotropin hormones on the expression of vitellogenin, gonadotropin subunits and gonadotropin receptors in cinnamon clownfish, Amphiprion melanopus.

Comp Biochem Physiol B Biochem Mol Biol 2012 Aug 26;162(4):73-80. Epub 2012 Apr 26.

Division of Marine Environment & BioScience, Korea Maritime University, Busan 606-791, Republic of Korea.

Gonadotropins (GTHs) are the key regulators of reproduction in vertebrates. The present study investigated autoregulatory effects of gonadotropins, using recombinant FSH (rFSH) and LH (rLH) in cinnamon clownfish (Amphiprion melanopus). Experiments were carried out to investigate the actions of cinnamon clownfish rFSH and rLH on expression of GTH subunits, GTH receptors, and vitellogenin (Vtg) mRNA in vivo and in vitro. Read More

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http://dx.doi.org/10.1016/j.cbpb.2012.04.006DOI Listing
August 2012
53 Reads

A goose-type lysozyme gene in Japanese scallop (Mizuhopecten yessoensis): cDNA cloning, mRNA expression and promoter sequence analysis.

Comp Biochem Physiol B Biochem Mol Biol 2012 May 17;162(1-3):34-43. Epub 2012 Feb 17.

College of Life Science, Liaoning Normal University, Dalian, 116029, PR China.

Lysozyme is an important component of the immune response against bacteria that is characterized by its ability to break down bacterial cell-walls. We constructed a high-quality cDNA library from mantle tissue of adult Japanese scallop (Mizuhopecten yessoensis). The EST which is high homology with g-type lysozyme genes of other species was found in the cDNA library. Read More

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http://dx.doi.org/10.1016/j.cbpb.2012.02.002DOI Listing
May 2012
15 Reads

Evidence for transcriptional regulation of the urea transporter in the gill of the Gulf toadfish, Opsanus beta.

Comp Biochem Physiol B Biochem Mol Biol 2011 Oct 30;160(2-3):72-80. Epub 2011 Jun 30.

Department of Biology, University of Ottawa, Ottawa, Ontario, Canada.

Ureotelic Gulf toadfish (Opsanus beta) do not excrete urea continuously; instead, urea is accumulated internally until a branchial urea transport mechanism is activated to facilitate the excretion of urea in distinct pulses. This unusual pulsatile urea excretion pattern is regulated, in part, by permissive declines in circulating cortisol concentrations. The current study examined toadfish urea transporter (tUT) and glucocorticoid receptor (GR) transcript levels in toadfish gill following chronic (days) and acute (hours) changes in corticosteroid activity. Read More

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http://dx.doi.org/10.1016/j.cbpb.2011.06.004DOI Listing
October 2011
40 Reads

Molecular pathways involved in the cyclic activity of frog (Pelophylax esculentus) Harderian gland: Influence of temperature and testosterone.

Comp Biochem Physiol B Biochem Mol Biol 2011 Jan 25;158(1):71-6. Epub 2010 Sep 25.

Dipartimento di Scienze della Vita, Seconda Università di Napoli, via Vivaldi 43, 81100 Caserta, Italy.

The Harderian gland of Pelophylax esculentus (previously: Rana esculenta) shows seasonal secretory activity changes. Specifically, the secretory activity reaches a maximum during the hottest months, i.e. Read More

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http://dx.doi.org/10.1016/j.cbpb.2010.09.007DOI Listing
January 2011
10 Reads

Identification of parvalbumin-like protein as a major protein of common carp (Cyprinus carpio L) spermatozoa which appears during final stage of spermatogenesis.

Comp Biochem Physiol B Biochem Mol Biol 2010 Oct 1;157(2):220-7. Epub 2010 Jul 1.

Department of Gamete and Embryo Biology, Semen Biology Group, Institute of Animal Reproduction and Food Research, 10-747 Olsztyn, Poland.

Parvalbumin is well known as the major fish allergen that is typically present in high amounts in muscles, where it functions in calcium buffering and is involved in the relaxation process in fast-twitch muscles. We show in our current study that parvalbumin-like protein is present in high amounts in carp spermatozoa. It is the first report to demonstrate the presence of parvalbumin-like protein in fish spermatozoa. Read More

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http://dx.doi.org/10.1016/j.cbpb.2010.06.007DOI Listing
October 2010
13 Reads

Carbohydrates and glycoforms of the major egg perivitellins from Pomacea apple snails (Architaenioglossa: Ampullariidae).

Comp Biochem Physiol B Biochem Mol Biol 2010 Sep 13;157(1):66-72. Epub 2010 May 13.

Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CONICET CCT La Plata-Universidad Nacional de La Plata, 60 y 120, (1900) La Plata, Argentina.

To better understand how glycans contribute to the multiple roles of perivitellins in embryo development, the carbohydrate moieties and glycoforms of the carotenoglycoproteins ovorubin and scalarin from the eggs of Pomaceacanaliculata (Lamarck, 1822) and Pomaceascalaris (d'Orbigny, 1835) were studied. All subunits of both proteins are glycosylated and appear to be glycoforms with isoelectric points ranging from approximately 5.3 to approximately 9. Read More

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http://dx.doi.org/10.1016/j.cbpb.2010.05.004DOI Listing
September 2010
9 Reads

Functional characterization of the chicken cationic amino acid transporter-2 isoforms.

Comp Biochem Physiol B Biochem Mol Biol 2010 Aug 23;156(4):279-86. Epub 2010 Apr 23.

Department of Animal and Avian Sciences, University of Maryland, College Park, 20742, USA.

Utilization of lysine and arginine by mammalian skeletal muscle is due in large part to the system y(+) cationic amino acid transporters (CAT), particularly CAT-2A. The chicken CAT-2 (cCAT-2) gene is alternatively spliced to produce three isoforms (cCAT-2A/B/C). Chicken (Gallus gallus) CAT-2 isoforms were transiently and stably expressed in mammalian cell lines to determine cCAT-2 isoform cellular localization and transport properties. Read More

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http://dx.doi.org/10.1016/j.cbpb.2010.04.005DOI Listing
August 2010
6 Reads

Cloning and characterization of the activin like receptor 1 homolog (Pf-ALR1) in the pearl oyster, Pinctada fucata.

Comp Biochem Physiol B Biochem Mol Biol 2010 Jul 10;156(3):158-67. Epub 2010 Mar 10.

Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, Beijing 100084, China.

The signal transduction mechanisms in mollusks are still elusive since the genome information is incomplete and cell lines are not available. In previous study, we cloned a highly conserved Smad3 homolog (designated as Pf-Smad3) from the pearl oyster, Pinctada fucata. It seems that transforming growth factor beta (TGFbeta) signaling may play similar roles in the oyster as in vertebrate. Read More

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http://dx.doi.org/10.1016/j.cbpb.2010.02.012DOI Listing
July 2010
9 Reads

Dietary protein hydrolysates and free amino acids affect the spatial expression of peptide transporter PepT1 in the digestive tract of Atlantic cod (Gadus morhua).

Comp Biochem Physiol B Biochem Mol Biol 2010 May 6;156(1):48-55. Epub 2010 Feb 6.

Department of Biology, University of Bergen, Norway.

The effects of dietary inclusions of size-fractionated peptides and free amino acids (FAAs) on Peptide Transporter 1 (PepT1) mRNA levels were assessed along the length of the intestine of juvenile Atlantic cod (Gadus morhua). Five groups of fish (10-15g) were fed for 46days on diets containing approximately 42% protein, provided either as fish meal (FM, control diet) or as a combination of FM with whole fish hydrolysate (FH), retenate after ultrafiltration of FH (UFR), nanofiltered retenate of FH (NFR), or a mix of FAAs, at a 30% level of FM substitution. PepT1 mRNA expression was assessed in pyloric caeca (S1) and the remainder of the intestine divided into four equally long segments (S2-S5). Read More

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http://dx.doi.org/10.1016/j.cbpb.2010.02.002DOI Listing
May 2010
12 Reads

Purification, characterization and cDNA cloning of a novel lectin from the jellyfish Nemopilema nomurai.

Comp Biochem Physiol B Biochem Mol Biol 2010 May 25;156(1):12-8. Epub 2010 Jan 25.

Department of Marine Bioscience, Fukui Prefectural University, Obama, Japan.

A lectin (designated NnL) from a jellyfish Nemopilema nomurai was purified by ion-exchange chromatography followed by affinity chromatography. The apparent molecular mass of NnL was 28kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing and nonreducing conditions. NnL agglutinated horse erythrocytes, the gram-positive bacterium Bacillus subtilis and gram-negative bacterium Escherichia coli K12. Read More

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http://dx.doi.org/10.1016/j.cbpb.2010.01.008DOI Listing
May 2010
10 Reads

Allometric scaling of fatty acyl chains in fowl liver, lung and kidney, but not in brain phospholipids.

Comp Biochem Physiol B Biochem Mol Biol 2010 Mar 2;155(3):301-8. Epub 2009 Dec 2.

Kaposvár University, Faculty of Animal Science, Laboratory of Animal Product Qualification, 7400 Kaposvár, Guba S. u. 40., Hungary.

The phospholipid (PL) fatty acyl chain (FA) composition (mol%) was determined in the kidney, liver, lung and brain of 8 avian species ranging in body mass from 150g (Japanese quail, Coturnix coturnix japonica) to 19kg (turkey, Meleagris gallopavo). In all organs except the brain, docosahexaenoic acid (C22:6 n3, DHA) was found to show a negative allometric scaling (allometric exponent: B=-0.18; -0. Read More

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http://dx.doi.org/10.1016/j.cbpb.2009.11.018DOI Listing
March 2010
7 Reads

The primary structure of a novel riboflavin-binding protein of emu (Dromaius novaehollandiae).

Comp Biochem Physiol B Biochem Mol Biol 2009 May 10;153(1):95-100. Epub 2009 Feb 10.

Department of Fermentation Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture, Japan.

Emu riboflavin-binding protein (RBP) was purified from egg white and yolk, and its N-terminal amino acid sequence was determined. The molecular mass of emu RBP was estimated at approximately 48 and 45 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, i.e. Read More

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http://dx.doi.org/10.1016/j.cbpb.2009.02.004DOI Listing
May 2009
10 Reads

Enzymatic activity of alpha-L-fucosidase and L-fucokinase across vertebrate animal species.

Comp Biochem Physiol B Biochem Mol Biol 2009 Aug 24;153(4):359-64. Epub 2009 Apr 24.

Department of Chemistry, Fort Hays State University, Hays, KS 67601 USA.

The oligosaccharide portion of glycoproteins is known to modulate protein structure, function, and turnover. Our laboratory is interested in the metabolism of L-fucose, a normal constituent of eukaryotic glycoproteins. L-fucose is unique in that it is the only levorotatory sugar utilized in mammalian systems. Read More

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http://dx.doi.org/10.1016/j.cbpb.2009.04.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413248PMC
August 2009
7 Reads

VGD and MLD-motifs containing heterodimeric disintegrin viplebedin-2 from Vipera lebetina snake venom. Purification and cDNA cloning.

Comp Biochem Physiol B Biochem Mol Biol 2009 Jul 16;153(3):253-60. Epub 2009 Mar 16.

National Institute of Chemical Physics and Biophysics, Akadeemia tee 23,Tallinn 12618, Estonia.

We have previously demonstrated that the fibrinolytic enzyme lebetase is synthesized with disintegrin-like domain that is cleaved posttranslationally (Siigur et al., 1996). Now we isolated a heterodimeric disintegrin viplebedin-2 containing this disintegrin-like part from Vipera lebetina venom using size-exclusion chromatography on Sephadex G-100 sf and HPLC on C18 column. Read More

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http://dx.doi.org/10.1016/j.cbpb.2009.03.002DOI Listing
July 2009
11 Reads

Molecular characterization of iron binding proteins, transferrin and ferritin heavy chain subunit, from the bumblebee Bombus ignitus.

Comp Biochem Physiol B Biochem Mol Biol 2009 Jan 16;152(1):20-7. Epub 2008 Sep 16.

College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Republic of Korea.

Transferrin and ferritin are iron-binding proteins involved in transport and storage of iron as part of iron metabolism. Here, we describe the cDNA cloning and characterization of transferrin (Bi-Tf) and the ferritin heavy chain subunit (Bi-FerHCH), from the bumblebee Bombus ignitus. Bi-Tf cDNA spans 2340 bp and encodes a protein of 706 amino acids and Bi-FerHCH cDNA spans 1393 bp and encodes a protein of 217 amino acids. Read More

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http://dx.doi.org/10.1016/j.cbpb.2008.09.082DOI Listing
January 2009
9 Reads

Phenotypic plasticity of gut structure and function during periods of inactivity in Apostichopus japonicus.

Comp Biochem Physiol B Biochem Mol Biol 2008 Jul 28;150(3):255-62. Epub 2008 Mar 28.

Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, PR China.

Apostichopus japonicus is a common sea cucumber that undergoes seasonal inactivity phases and ceases feeding during the summer months. We used this sea cucumber species as a model in which to examine phenotypic plasticity of the digestive tract in response to food deprivation. We measured the body mass, gross gut morphology and digestive enzyme activities of A. Read More

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http://dx.doi.org/10.1016/j.cbpb.2008.03.011DOI Listing
July 2008
11 Reads

Identification, characterization and expression of cathelicidin in the pouch young of tammar wallaby (Macropus eugenii).

Comp Biochem Physiol B Biochem Mol Biol 2008 Mar 11;149(3):524-33. Epub 2008 Jan 11.

Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of Veterinary Science, University of Sydney, NSW, 2006, Australia.

Antimicrobial peptides, such as cathelicidin, are an evolutionarily old defense system. However they have more complex actions than just simply their antimicrobial effects, including immunoregulation and interaction with the adaptive immune system. In this study we have characterized several novel cathelicidin-like peptides from the tammar wallaby (Macropus eugenii). Read More

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http://dx.doi.org/10.1016/j.cbpb.2007.12.002DOI Listing
March 2008
22 Reads

Vitellogenesis in both sexes of gonochoristic mud shrimp, Upogebia major (Crustacea): analyses of vitellogenin gene expression and vitellogenin processing.

Comp Biochem Physiol B Biochem Mol Biol 2008 Apr 17;149(4):589-98. Epub 2007 Dec 17.

Laboratory of Animal Behavior and Evolution, Graduate School of Natural Science and Technology, Okayama University, Tsushima 3-1-1, Okayama 700-8530, Japan.

The mud shrimp, Upogebia major is a gonochoristic species with distinct sexual dimorphism; however, the male has the "ovarian part of testis" in the gonad and mature-looking eggs appear in a similar reproductive cycle to the female. Vitellogenesis of U. major was investigated focusing on the characterization of vitellogenin (Vg) gene expression and Vg processing. Read More

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http://dx.doi.org/10.1016/j.cbpb.2007.12.003DOI Listing
April 2008
25 Reads

Goat vitronectin: characterization and binding to Staphylococcus aureus.

Comp Biochem Physiol B Biochem Mol Biol 2008 Mar 17;149(3):410-8. Epub 2007 Nov 17.

Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, India.

Vitronectin (Vn) is a multifunctional protein present in plasma and in the extracellular matrix. Previous studies have demonstrated binding of many bacteria to human Vn. In this study, we have characterized goat Vn and studied its interaction with S. Read More

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http://dx.doi.org/10.1016/j.cbpb.2007.11.001DOI Listing
March 2008
7 Reads

Carotenoids and throat pouch coloration in the great frigatebird (Fregata minor).

Comp Biochem Physiol B Biochem Mol Biol 2008 Feb 30;149(2):370-7. Epub 2007 Oct 30.

Department of Biology, University of Miami, Coral Gables, FL 33146, USA.

Carotenoid pigments are a common source of red, orange, and yellow coloration in vertebrates. Animals cannot manufacture carotenoids and therefore must obtain them in their diet to produce carotenoid-based coloration. Male great frigatebirds (Fregata minor) display a bright red inflated gular pouch as part of their elaborate courtship display. Read More

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http://dx.doi.org/10.1016/j.cbpb.2007.10.010DOI Listing
February 2008
5 Reads

The role of GlcNAc in formation and function of extracellular matrices.

Authors:
Bernard Moussian

Comp Biochem Physiol B Biochem Mol Biol 2008 Feb 26;149(2):215-26. Epub 2007 Oct 26.

Max-Planck-Institut für Entwicklungsbiologie, Spemannstr. 35, 72076 Tübingen, Germany.

N-acetylglucosamine (GlcNAc) is an abundant hexose that as a monomer or as part of macromolecules plays multiple roles in eukaryotic cells. Especially as a residue of oligo- and polysaccharides and conjugated with lipids and proteins, GlcNAc contributes to the function and architecture of extracellular matrices. Several human disorders caused by mutations in genes coding for enzymes dealing with GlcNAc have been identified. Read More

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http://dx.doi.org/10.1016/j.cbpb.2007.10.009DOI Listing
February 2008
8 Reads

Increase of stress resistance and lifespan of Caenorhabditis elegans by quercetin.

Comp Biochem Physiol B Biochem Mol Biol 2008 Feb 16;149(2):314-23. Epub 2007 Oct 16.

Institute of Toxicology, Heinrich-Heine University, Düsseldorf, Germany.

The health beneficial effects of a diet rich in fruits and vegetables are, at least in part, attributed to polyphenols that are present in many herbal edibles. Although many in vitro studies revealed a striking variety of biochemical and pharmacological properties data about the beneficial effects of polyphenols in whole organisms, especially with respect to ageing, are quite limited. We used the well established model organism Caenorhabditis elegans to elucidate the protective effects of quercetin, the main representative of the flavonol class of polyphenols, in vivo. Read More

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http://dx.doi.org/10.1016/j.cbpb.2007.10.004DOI Listing
February 2008
8 Reads

Epiphragmin, the major protein of epiphragm mucus from the vineyard snail, Cernuella virgata.

Comp Biochem Physiol B Biochem Mol Biol 2007 Oct 31;148(2):192-200. Epub 2007 May 31.

CSIRO Molecular & Health Technologies, Sydney Laboratory, P.O.Box 184, North Ryde, NSW 1670, Australia.

The organic fraction of epiphragm mucus from the snail Cernuella virgata (Mollusca: Helicidae) consists predominantly of protein (17-23 dry wt.%) rather than carbohydrate (< or =0.4-2. Read More

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http://dx.doi.org/10.1016/j.cbpb.2007.05.009DOI Listing
October 2007
6 Reads

The response of digestive proteases to abrupt salinity decrease in the euryhaline sparid Sparus aurata L.

Comp Biochem Physiol B Biochem Mol Biol 2007 Jun 3;147(2):156-63. Epub 2007 Mar 3.

Department of Biochemistry & Biotechnology, University of Thessaly, 26 Ploutonos Street, 41221 Larissa, Greece.

The response of the digestive proteases to abrupt salinity change was studied in juvenile gilthead sea bream (Sparus aurata) for 15 days after transfer from 33 per thousand to 21 per thousand. Salinity decrease affected significantly neither the activity of total acid proteases in stomach, nor the activities of total alkaline proteases and major serine proteases--trypsin and chymotrypsin--in the alkaline part of the intestine. The activity of the major proteases was significantly different between the alkaline segments of the intestine, with the posterior intestine presenting the highest activities followed by the pyloric caeca. Read More

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http://dx.doi.org/10.1016/j.cbpb.2006.12.021DOI Listing
June 2007
18 Reads

Tissue distribution and characterization of cholinesterase activity in six earthworm species.

Comp Biochem Physiol B Biochem Mol Biol 2007 Jun 7;147(2):340-6. Epub 2007 Feb 7.

Laboratoire de Toxicologie Environnementale, UMR 406 INRA/UAPV Ecologie des invertébrés Site AGROPARC 84914 Avignon Cedex 09 France.

To validate cholinesterase activity as a biomarker of pesticide exposure, we characterized the tissue distribution (whole body, nervous tissue and crop/gizzard), activity at two seasons of cholinesterase in six different species of earthworms collected in an unpolluted field: Lumbricus terrestris, Lumbricus castaneus, Aporrectodea nocturna, Aporrectodea caliginosa, Allolobophora chlorotica and Aporrectodea rosea. The major part of total cholinesterase activity was found in the nervous tissue while activity in crop/gizzard was weak. The level of the total cholinesterase activity was stable for each species considered throughout the year (spring and autumn). Read More

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http://dx.doi.org/10.1016/j.cbpb.2007.01.022DOI Listing
June 2007
7 Reads