877,075 results match your criteria Combination Products in Therapy [Journal]


The occipitoatlantal capsular ligaments are the primary stabilizers of the occipitoatlantal joint in the craniocervical junction: a finite element analysis.

J Neurosurg Spine 2019 Feb 15:1-9. Epub 2019 Feb 15.

2Department of Neurosurgery, Division of Pediatric Neurosurgery, University of Utah, Primary Children's Hospital, Salt Lake City, Utah.

OBJECTIVEThere is contradictory evidence regarding the relative contribution of the key stabilizing ligaments of the occipitoatlantal (OA) joint. Cadaveric studies are limited by the nature and the number of injury scenarios that can be tested to identify OA stabilizing ligaments. Finite element (FE) analysis can overcome these limitations and provide valuable data in this area. Read More

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http://dx.doi.org/10.3171/2018.10.SPINE181102DOI Listing
February 2019

Validation of a methodology for neuro-urological and lumbosacral stimulation studies in domestic pigs: a humanlike animal model.

J Neurosurg Spine 2019 Feb 15:1-11. Epub 2019 Feb 15.

7Neurosurgery, Pellegrin Hospital, Bordeaux, Nouvelle Aquitaine; and.

OBJECTIVESpinal cord injuries (SCIs) result in loss of movement and sensory feedback, but also organ dysfunction. Nearly all patients with complete SCI lose bladder control and are prone to kidney failure if intermittent catheterization is not performed. Electrical stimulation of sacral spinal roots was initially considered to be a promising approach for restoring continence and micturition control, but many patients are discouraged by the need for surgical deafferentation as it could lead to a loss of sensory functions and reflexes. Read More

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http://dx.doi.org/10.3171/2018.11.SPINE18676DOI Listing
February 2019

Using the entrapped bioprocess as the pretreatment method for the drinking water treatment receiving eutrophic source water.

Environ Pollut 2019 Feb 4;248:57-65. Epub 2019 Feb 4.

Graduate Institute of Environmental Engineering, National Taiwan University, Taipei, 10617, Taiwan. Electronic address:

Control of organic matter, nutrients and disinfection byproduct formation is a major challenge for the drinking water treatment plants on Matsu Islands, Taiwan, receiving source water from the eutrophic reservoirs. A pilot entrapped biomass reactor (EBR) system was installed as the pretreatment process to reduce organic and nitrogen contents into the drinking water treatment plant. The effects of hydraulic retention time (HRT) and combination of preceding physical treatment (ultraviolet and ultrasound) on the treatment performance were further evaluated. Read More

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http://dx.doi.org/10.1016/j.envpol.2019.01.128DOI Listing
February 2019

Changes in blood pressure associated with lead, manganese, and selenium in a Bangladeshi cohort.

Environ Pollut 2019 Feb 7;248:28-35. Epub 2019 Feb 7.

Division of Epidemiology and Biostatistics, University of Illinois at Chicago, School of Public Health, Chicago, IL, USA. Electronic address:

Background: Heavy metal contamination is widespread in Bangladesh. Previous studies have observed lead increases blood pressure over time. However, the role of other metal contaminants and essential micronutrients, which could also adversely affect blood pressure or act as protective factors, is understudied. Read More

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http://dx.doi.org/10.1016/j.envpol.2019.01.129DOI Listing
February 2019

The value of delta neutrophil index in neonatal sepsis diagnosis, follow-up and mortality prediction.

Early Hum Dev 2019 Feb 13;131:6-9. Epub 2019 Feb 13.

Division of Neonatology, Department of Pediatrics, University of Health Sciences, Etlik Zubeyde Hanim Women's Health Teaching and Research Hospital, 06010, Ankara, Turkey; Division of Neonatology, Department of Pediatrics, Yildirim Beyazit University, 06800, Ankara, Turkey.

Background: The complete blood cell count (CBC) and peripheral blood smear were the most commonly ordered tests for the diagnosis of neonatal sepsis. Delta neutrophil index (DNI) shows leucocyte differentiation and calculated while CBC is performed.

Aims: We aimed to evaluate the value of DNI in neonatal sepsis. Read More

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http://dx.doi.org/10.1016/j.earlhumdev.2019.02.003DOI Listing
February 2019

Adjuvant androgen deprivation therapy for poor-risk, androgen receptor-positive salivary duct carcinoma.

Eur J Cancer 2019 Feb 13;110:62-70. Epub 2019 Feb 13.

Department of Medical Oncology, Radboud University Medical Center, Geert Grooteplein Zuid 8, 6525 GA Nijmegen, the Netherlands. Electronic address:

Aim: Salivary duct carcinoma (SDC), an aggressive subtype of salivary gland cancer, is androgen receptor (AR)-positive in 67-96% of cases. In patients with locally recurrent and metastatic (R/M) AR-positive SDC, androgen deprivation therapy (ADT) has an overall response rate of 18-64.7%. Read More

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http://dx.doi.org/10.1016/j.ejca.2018.12.035DOI Listing
February 2019

Unsaturated fatty acids from flaxseed oil and exercise modulate GPR120 but not GPR40 in the liver of obese mice: a new anti-inflammatory approach.

J Nutr Biochem 2018 Dec 12;66:52-62. Epub 2018 Dec 12.

Nutritional Genomics Laboratory, LabGeN, School of Applied Sciences, UNICAMP, São Paulo, Brazil; Nutrigenomics and Lipids Research Center, CELN, School of Applied Sciences, UNICAMP, São Paulo, Brazil. Electronic address:

GPR120 and GPR40 were recently reported as omega-3 (ω3) receptors with anti-inflammatory properties. Physical exercise could increase the expression of these receptors in the liver, improving hepatic metabolism in obesity and type 2 diabetes. Our aim was to investigate GPR120/40 in the liver of lean and obese mice after acute or chronic physical exercise, with or without the supplementation of ω3 rich flaxseed oil (FS), as well as assess the impact of exercise and FS on insulin signaling and inflammation. Read More

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http://dx.doi.org/10.1016/j.jnutbio.2018.12.003DOI Listing
December 2018

Force-induced decline of TEA domain family member 1 contributes to osteoclastogenesis via regulation of Osteoprotegerin.

Arch Oral Biol 2019 Jan 31;100:23-32. Epub 2019 Jan 31.

Department of Orthodontics, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, China. Electronic address:

Objective: This study aims to investigate the responsiveness of transcription factor TEA domain family member 1 (TEAD1) to mechanical force and its impact on osteoclastogenesis as well as expression of Osteoprotegerin (OPG), an inhibitor for osteoclastogenesis playing crucial roles in mechanical stress-induced bone remodeling and orthodontic tooth movement (OTM).

Methods: We first analyzed the correlation between several transcription factors and OPG expression in human periodontal ligament cells (PDLCs). Then dynamic expression changes of TEAD1 with force application were analyzed due to its high correlation with OPG. Read More

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http://dx.doi.org/10.1016/j.archoralbio.2019.01.020DOI Listing
January 2019

Efficacy of vermitechnology integration with Upflow Anaerobic Sludge Blanket (UASB) and activated sludge for metal stabilization: A compliance study on fractionation and biosorption.

J Environ Manage 2019 Feb 13;236:603-612. Epub 2019 Feb 13.

Discipline of Earth Science, Indian Institute of Technology Gandhinagar, 382355, Gujarat, India. Electronic address:

Efficacy of vermi-transformation for metal partitioning and transformation from Upflow Anaerobic Sludge Blanket (UASB) and Activated Sludge (AS) was investigated. Sludge samples were mixed with cow dung (CD) in two combinations (1:1 (UASB/AS:CD)) & (2:1(UASB/AS: CD)). Fractionation study revealed that Zn, Cd & Pb were associated with reducible fractions, and Cr, Cu with oxidizable fractions. Read More

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http://dx.doi.org/10.1016/j.jenvman.2019.01.006DOI Listing
February 2019

S. epidermidis strains from artisanal cheese made from unpasteurized milk in Poland - Genetic characterization of antimicrobial resistance and virulence determinants.

Int J Food Microbiol 2019 Feb 7;294:55-59. Epub 2019 Feb 7.

Chair of Industrial and Food Microbiology, Faculty of Food Science, University of Warmia and Mazury, Plac Cieszyński 1, 10-726 Olsztyn, Poland.

In Poland artisanal cheese production is an important local economic activity. Artisanal cheese is usually produced using raw cow's milk, animal rennet and salt, without the addition of starter cultures. Coagulase negative staphylococci (CoNS) are often present in artisanal cheeses. Read More

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http://dx.doi.org/10.1016/j.ijfoodmicro.2019.02.004DOI Listing
February 2019

Metabolic shift of Staphylococcus aureus under sublethal dose of methicillin in the presence of glucose.

J Pharm Biomed Anal 2019 Feb 8;167:140-148. Epub 2019 Feb 8.

Department of Chemistry and Biochemistry, Miami University, Oxford, OH, 45056, USA; Department of Human Sciences, The Ohio State University, Columbus, OH, 43210, USA. Electronic address:

Traditional strategies in developing novel drugs to treat antibiotic-resistant S. aureus have not been very successful to date, therefore, there is an urgent need for creative usage of existing agents that can treat and control S. aureus infection. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.02.010DOI Listing
February 2019

ERK Inhibitor Enhances Everolimus Efficacy through the Attenuation of dNTP Pools in Renal Cell Carcinoma.

Mol Ther Nucleic Acids 2019 Jan 10;14:550-561. Epub 2019 Jan 10.

Department of Urology and Andrology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China. Electronic address:

The clinical efficiency of everolimus, an mammalian target of rapamycin (mTOR) inhibitor, is palliative as sequential or second-line therapy for renal cell carcinoma (RCC). However, the limited response of everolimus in RCC remains uncertain. In the present study, everolimus-resistant RCC models were established to understand the mechanisms and to seek combination approaches. Read More

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http://dx.doi.org/10.1016/j.omtn.2019.01.001DOI Listing
January 2019

A pan-genotypic Hepatitis C Virus NS5A amplification method for reliable genotyping and resistance testing.

J Clin Virol 2019 Jan 30;113:8-13. Epub 2019 Jan 30.

Institute of Virology, Heinrich-Heine-University, University Hospital, Düsseldorf, Germany.

Background: Chronic infection with the Hepatitis C Virus (HCV) is associated with the risk of progressive liver disease. Although, HCV treatment options and viral cure rates have tremendously increased over the last decade, all currently licensed combination therapies contain inhibitors of the replication complex NS5A. Resistance-associated substitutions (RAS) in NS5A can limit the efficacy of therapy; however, resistance testing is routinely not recommended for all patients. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13866532193002
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http://dx.doi.org/10.1016/j.jcv.2019.01.012DOI Listing
January 2019
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Indocyanine green/doxorubicin-encapsulated functionalized nanoparticles for effective combination therapy against human MDR breast cancer.

Colloids Surf B Biointerfaces 2019 Feb 5;177:294-305. Epub 2019 Feb 5.

Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 300, Taiwan. Electronic address:

To overcome low therapeutic efficacy of chemotherapy against multidrug resistance (MDR) breast cancer, a combination therapy system based upon functionalized polymer nanoparticles comprising poly(γ-glutamic acid)-g-poly(lactic-co-glycolic acid) (γ-PGA-g-PLGA) as the major component was developed. The NPs were loaded with doxorubicin (DOX) and indocyanine green (ICG) for dual modality cancer treatment and coated with cholesterol-PEG (C-PEG) for MDR abrogation in treatment of human MDR breast cancer. The in vitro cellular uptake of the DOX/ICG loaded nanoparticles (DI-NPs) by MDR cancer cells was significantly enhanced owing to effective inhibition of the P-gp activity by C-PEG and γ-PGA receptor-mediated endocytosis. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2019.02.001DOI Listing
February 2019

Chk1 inhibition enhances cisplatin cytotoxicity and overcomes cisplatin resistance in small cell lung cancer by promoting mitotic cell death.

J Thorac Oncol 2019 Feb 13. Epub 2019 Feb 13.

Department of Oncology, Georgetown University Medical Center, Washington, DC. Electronic address:

Introduction: Platinum-based chemotherapy remains the standard treatment for patients with small cell lung cancer (SCLC), but the benefit of the treatment is often hampered by rapid development of drug resistance. Thus far, there is no targeted therapy available for SCLC. Over 90% of SCLC tumors harbor mutations in the tumor suppressor gene p53, an important DNA damage checkpoint regulator, and these tumor cells rely predominantly on the checkpoint kinases to control DNA damage response. Read More

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http://dx.doi.org/10.1016/j.jtho.2019.01.028DOI Listing
February 2019

The Combination of MEK inhibitor with Immunomodulatory Antibodies Targeting PD-1 and PD-L1 Results in Prolonged Survival in Kras/p53-Driven Lung Cancer.

J Thorac Oncol 2019 Feb 13. Epub 2019 Feb 13.

Section of Medical Oncology, Department of Internal Medicine; Developmental Therapeutics Translational Research Program, Yale Comprehensive Cancer Center, New Haven, Connecticut , USA. Electronic address:

Purpose: To characterize the tumor-infiltrating immune cells population in Kras/p53-driven lung tumors and to evaluate the combinatorial anti-tumor effect with MEK inhibitor (MEKi), trametinib, and immunomodulatory monoclonal antibodies (mAbs) targeting either programmed cell death protein-1 (PD-1) or programmed cell death protein ligand 1 (PD-L1) in vivo.

Experimental Design: Trp53;Kras;Rosa26 (PKL) genetically engineered mice were utilized to develop autochthonous lung tumors with intratracheal delivery of adenoviral Cre recombinase. Using these tumor-bearing lungs, tumor-infiltrating immune cells were characterized by both mass cytometry and flow cytometry. Read More

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http://dx.doi.org/10.1016/j.jtho.2019.02.004DOI Listing
February 2019

Viral genome integration of canine papillomavirus 16.

Papillomavirus Res 2019 Feb 13. Epub 2019 Feb 13.

Department of Pathology, Georgetown University Medical Center, Washington, DC, USA.

Papillomaviruses infect humans and animals, most often causing benign proliferations on skin or mucosal surfaces. Rarely, these infections persist and progress to cancer. In humans, this transformation most often occurs with high-risk papillomaviruses, where viral integration is a critical event in carcinogenesis. Read More

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http://dx.doi.org/10.1016/j.pvr.2019.02.002DOI Listing
February 2019

Leukotoxic activity of Fusobacterium necrophorum of cattle origin.

Anaerobe 2019 Feb 13. Epub 2019 Feb 13.

Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66506, USA. Electronic address:

Fusobacterium necrophorum is a Gram negative, rod-shaped and aero tolerant anaerobe. In animals, it is an opportunistic pathogen frequently associated with necrotic infections, generally called necrobacillosis, such as calf diphtheria, foot rot and liver abscesses in cattle. Two subspecies exist: subsp. Read More

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http://dx.doi.org/10.1016/j.anaerobe.2019.02.009DOI Listing
February 2019

Towards the first targeted therapy for triple-negative breast cancer: repositioning of clofazimine as a chemotherapy-compatible selective Wnt pathway inhibitor.

Cancer Lett 2019 Feb 13. Epub 2019 Feb 13.

Department of Cell Physiology and Metabolism, Translational Research Centre in Oncohaematology, Faculty of Medicine, University of Geneva, Geneva, Switzerland; School of Biomedicine, Far Eastern Federal University, Vladivostok, Russia. Electronic address:

Wnt signaling is overactivated in triple-negative breast cancer (TNBC) and several other cancers, and its suppression emerges as an effective anticancer treatment. However, no drugs targeting the Wnt pathway exist on the market nor in advanced clinical trials. Here we provide a comprehensive body of preclinical evidence that an anti-leprotic drug clofazimine is effective against TNBC. Read More

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http://dx.doi.org/10.1016/j.canlet.2019.02.018DOI Listing
February 2019

Identification and characterization of small molecule inhibitors of the ubiquitin ligases Siah1/2 in melanoma and prostate cancer cells.

Cancer Lett 2019 Feb 13. Epub 2019 Feb 13.

Cancer Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, 92037, USA. Electronic address:

Inhibition of ubiquitin ligases with small molecule remains a very challenging task, given the lack of catalytic activity of the target and the requirement of disruption of its interactions with other proteins. Siah1/2, which are E3 ubiquitin ligases, are implicated in melanoma and prostate cancer and represent high-value drug targets. We utilized three independent screening approaches in our efforts to identify small-molecule Siah1/2 inhibitors: Affinity Selection-Mass Spectrometry, a protein thermal shift-based assay and an in silico based screen. Read More

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http://dx.doi.org/10.1016/j.canlet.2019.02.012DOI Listing
February 2019

A comparison of apremilast monotherapy and combination therapy for psoriatic arthritis in a real life setting: data from the Leeds Combined Psoriatic Service.

J Am Acad Dermatol 2019 Feb 13. Epub 2019 Feb 13.

NIHR Leeds Biomedical Research Centre, Leeds Teaching Hospitals NHS Trust, Leeds, UK; Leeds Institute of Rheumatic and Musculoskeletal Medicine, University of Leeds, UK.

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http://dx.doi.org/10.1016/j.jaad.2019.02.014DOI Listing
February 2019

Sequential activation of anticancer therapy triggered by tumor microenvironment-selective imaging.

J Control Release 2019 Feb 13. Epub 2019 Feb 13.

College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Republic of Korea. Electronic address:

The combination of imaging and anticancer therapy has recently emerged as a promising strategy. However, nonspecific imaging signals and distribution of anticancer drugs at normal tissues limit the specificity of the combination therapy. To overcome the challenges, we designed a system which can selectively visualize cancer tissues and initiate the subsequent action of therapeutic molecules in tumor microenvironment. Read More

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http://dx.doi.org/10.1016/j.jconrel.2019.02.012DOI Listing
February 2019

Clinical Manifestations, Prognosis And Vaccination Status Of Patients With Rubella Virus-Associated Uveitis.

Am J Ophthalmol 2019 Feb 13. Epub 2019 Feb 13.

Department of Ophthalmology, Erasmus University Medical Center, Rotterdam, The Netherlands.

Purpose: To assess the clinical and laboratory manifestations and vaccination status of uveitis patients positive for Rubella virus (RV) in aqueous humor and investigate its relationship to Fuchs Uveitis Syndrome (FUS).

Methods: Retrospective study of all uveitis patients, positive for RV in aqueous humor analysis (polymerase chain reaction (PCR) and/or Goldmann-Witmer coefficient (GWC)) between January 2010 and October 2016 at the ophthalmology departments in the Erasmus Medical Center (Rotterdam) and University Medical Center Utrecht. Outcomes of aqueous analyses of FUS patients during this period were assessed. Read More

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http://dx.doi.org/10.1016/j.ajo.2019.02.002DOI Listing
February 2019

Nox2-mediated platelet activation by glycoprotein (GP) VI: effect of rivaroxaban alone and in combination with aspirin.

Biochem Pharmacol 2019 Feb 13. Epub 2019 Feb 13.

Department of Internal Medicine and Medical Specialties, Sapienza University of Rome, Rome, Italy. Electronic address:

Factor Xa (FXa) has been reported to activate platelet via interaction with glycoprotein (GP) VI but the underlying mechanism has not been fully elucidated. We investigated if Nox2-derived oxidative stress is implicated in FXa-induced platelet aggregation (PA), and the effect of a FXa inhibitor, namely rivaroxaban, with or without aspirin (ASA), on PA. We performed an in vitro study measuring convulxin-induced PA, thromboxane (Tx) B and isoprostanes biosynthesis, soluble Nox2-dp (sNox2-dp), a marker of Nox2 activation, soluble GPVI (sGPVI) and PLA activation in platelets from healthy subjects (n=5) added with and without a Nox2 inhibitor. Read More

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http://dx.doi.org/10.1016/j.bcp.2019.02.016DOI Listing
February 2019

Placental Growth Factor Blunts Uterine Artery Responses to Angiotensin II.

BJOG 2019 Feb 16. Epub 2019 Feb 16.

Department of Obstetrics and Gynecology, Baylor College of Medicine and Texas Children's Hospital-Pavilion for Women, Houston, TX, United States.

Objective: Changes in maternal serum concentration of placental growth factor (PlGF) and vascular response to intravascular infusion of Angiotensin II (Ang II) follow a bell-shaped curve pattern during gestation. This study evaluates the effects of PlGF and soluble vascular endothelial growth factor receptor-1 (sFlt-1) on responses of human uterine arteries (UA) to Ang II.

Design: Experimental. Read More

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http://dx.doi.org/10.1111/1471-0528.15656DOI Listing
February 2019

A multicenter phase II study of temozolomide plus disulfiram and copper for recurrent temozolomide-resistant glioblastoma.

J Neurooncol 2019 Feb 15. Epub 2019 Feb 15.

Washington University School of Medicine, St. Louis, MO, USA.

Purpose: Preclinical studies have suggested promising activity for the combination of disulfiram and copper (DSF/Cu) against glioblastoma (GBM) including re-sensitization to temozolomide (TMZ). A previous phase I study demonstrated the safety of combining DSF/Cu with adjuvant TMZ for newly diagnosed GBM. This phase II study aimed to estimate the potential effectiveness of DSF/Cu to re-sensitize recurrent GBM to TMZ. Read More

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http://dx.doi.org/10.1007/s11060-019-03125-yDOI Listing
February 2019

Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy.

Methods Mol Biol 2019 ;1948:247-260

Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, Germany.

Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling is ideally suited to study structure, dynamics, and interactions of intrinsically disordered proteins as alpha-synuclein.Here we describe all steps required for a corresponding study: the spin labeling procedure, sample preparation, spectroscopic experimental procedure, and data analysis. Read More

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http://dx.doi.org/10.1007/978-1-4939-9124-2_19DOI Listing
January 2019

In Situ Peroxidase Labeling and Mass Spectrometry of Alpha-Synuclein in Rat Cortical Neurons.

Methods Mol Biol 2019 ;1948:235-246

Ann Romney Center for Neurologic Disease, Department of Neurology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.

In this chapter, we describe a novel ascorbate peroxidase (APEX)-based labeling method that in combination with mass spectrometry identifies proteins in the immediate vicinity of αSyn in living rat cortical neurons. To isolate these interactions, we transduced primary cortical neurons with a lentivirus encoding APEX2 tagged to the C-terminus of alpha-synuclein (αSyn) and under the control of a synapsin promoter. Neural protein lysates were then incubated with streptavidin magnetic beads, washed, eluted from the beads, and digested overnight. Read More

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http://dx.doi.org/10.1007/978-1-4939-9124-2_18DOI Listing
January 2019

Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes.

Authors:
Matteo Rovere

Methods Mol Biol 2019 ;1948:123-143

Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.

α-Synuclein's physiology and pathology have been linked by numerous reports to its ability to bind and remodel membranes, especially at synaptic terminals. It is therefore critical for researchers investigating the determinants of these interactions to rely on methods capable of providing an accurate and complete physicochemical snapshot of the binding events. Circular dichroism (CD) and isothermal titration calorimetry (ITC) are established techniques for the study of binding equilibria in biological systems and, especially when used in combination, allow a thorough characterization of the protein-lipid interplay. Read More

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http://dx.doi.org/10.1007/978-1-4939-9124-2_11DOI Listing
January 2019

Association and gene-gene interaction analyses for polymorphic variants in CTLA-4 and FOXP3 genes: role in susceptibility to autoimmune thyroid disease.

Endocrine 2019 Feb 15. Epub 2019 Feb 15.

Department of Genetics, Osmania University, Hyderabad, Telangana, India.

Purpose: Polymorphic variants of cytotoxic T-lymphocyte antigen-4 (CTLA-4) and forkhead box protein P3 (FOXP3) genes are implicated in dysregulated immune homeostasis and autoimmune disorders. We analyzed the association between CTLA-4 rs231775 and FOXP3 rs3761548, rs3761549 polymorphisms and predisposition to autoimmune thyroid disease (AITD), inclusive of Hashimoto's thyroiditis (HT) and Graves' disease (GD) in South-Indian population.

Methods: A total of 355 AITD subjects (comprising 275 HT and 80 GD) and 285 randomly selected age- and sex-matched control subjects were genotyped for the aforementioned polymorphisms by PCR-RFLP method. Read More

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http://dx.doi.org/10.1007/s12020-019-01859-3DOI Listing
February 2019

How do we perform backward serial recall?

Mem Cognit 2019 Feb 15. Epub 2019 Feb 15.

MRC Cognition and Brain Sciences Unit, University of Cambridge, 15 Chaucer Road, Cambridge, CB2 7EF, UK.

Following Conrad (1965, Journal of Verbal Learning and Verbal Behavior, 4, 161-169) it is often assumed that backward verbal serial recall is performed by repeated forward scans through the list and then recalling the last remaining item. Direct evidence for this peel-off strategy is relatively weak, and there has to date been no examination of its potential role in the recall of spatial sequences. To examine the role of this strategy in both verbal and spatial domains, two experiments examined response output times for forward and backward recall. Read More

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http://dx.doi.org/10.3758/s13421-018-0889-2DOI Listing
February 2019

Dynamic gas extraction of iodine in combination with a silver triangular nanoplate-modified paper strip for colorimetric determination of iodine and of iodine-interacting compounds.

Mikrochim Acta 2019 Feb 15;186(3):188. Epub 2019 Feb 15.

Department of Chemistry, Lomonosov Moscow State University, Leninskie gory, 1/3, 119991, Moscow, Russia.

A method is described for sensitive and selective detection of iodine by using a paper strip modified with silver triangular nanoplates (AgTNPs). It is based on the extraction of iodine from a solution into a flow of air via dynamic gas extraction and transferring it through a reactive paper modified with AgTNPs. The interaction of AgTNPs with iodine results in a color change from blue to white. Read More

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http://dx.doi.org/10.1007/s00604-019-3300-5DOI Listing
February 2019

Combined and Singular Effects of Dietary PrimaLac® and Potassium Diformate (KDF) on Growth Performance and Some Physiological Parameters of Rainbow Trout (Oncorhynchus mykiss).

Probiotics Antimicrob Proteins 2019 Feb 15. Epub 2019 Feb 15.

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand.

The present study was conducted to assess the effects of combined and singular dietary administration of PrimaLac® and potassium diformate (KDF) on growth performance, feed utilization, digestive enzymes activity, and some physiological parameters of rainbow trout (Oncorhynchus mykiss) juvenile. Three hundred sixty rainbow trout juveniles (25 ± 1.8 g) were randomly stocked in 300-L tanks (30 fish/tank), and fed three times daily on a basal diet (control), diets incorporated with 12 g kg KDF (FT), 1. Read More

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http://dx.doi.org/10.1007/s12602-019-9523-2DOI Listing
February 2019

Efficacy of nintedanib and docetaxel in patients with advanced lung adenocarcinoma treated with first-line chemotherapy and second-line immunotherapy in the nintedanib NPU program.

Clin Transl Oncol 2019 Feb 15. Epub 2019 Feb 15.

M.D. Anderson Cancer Center, Madrid, Spain.

Purpose: Both nintedanib/docetaxel and anti-PD-1/PD-L1 immunotherapies have demonstrated efficacy as second-line treatment of patients with advanced lung adenocarcinoma. This is the first report on the efficacy of the nintedanib/docetaxel combination following first-line platinum-based chemotherapy and subsequent immunotherapy in a real-world setting.

Methods/patients: From May 2014 to December 2015, 390 patients in 108 Spanish centres enrolled in the nintedanib named patient use program. Read More

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http://dx.doi.org/10.1007/s12094-019-02053-7DOI Listing
February 2019

Complete protein assignment from sets of spectra recorded overnight.

J Biomol NMR 2019 Feb 15. Epub 2019 Feb 15.

Department of Chemistry and Molecular Biology, University of Gothenburg, 40530, Gothenburg, Sweden.

A flexible and scalable approach for protein NMR is introduced that builds on rapid data collection via projection spectroscopy and analysis of the spectral input data via joint decomposition. Input data may originate from various types of spectra, depending on the ultimate goal: these may result from experiments based on triple-resonance pulse sequences, or on TOCSY or NOESY sequences, or mixtures thereof. Flexible refers to the free choice of spectra for the joint decompositions depending on the purpose: assignments, structure, dynamics, interactions. Read More

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http://dx.doi.org/10.1007/s10858-019-00226-8DOI Listing
February 2019

Responses of olive plants exposed to different irrigation treatments in combination with heat shock: physiological and molecular mechanisms during exposure and recovery.

Planta 2019 Feb 15. Epub 2019 Feb 15.

Department of Life Science, Centre for Functional Ecology (CFE), University of Coimbra, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal.

Main Conclusion: A water-deficit period, leading to stomatal control and overexpression of protective proteins (sHSP and DHN), contributes to olive´s tolerance to later imposed stress episodes. Aquaporins modulation is important in olive recovery. Olive is traditionally cultivated in dry farming or in high water demanding irrigated orchards. Read More

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http://dx.doi.org/10.1007/s00425-019-03109-2DOI Listing
February 2019

Delivery Systems as Vital Tools in Drug Repurposing.

AAPS PharmSciTech 2019 Feb 15;20(3):116. Epub 2019 Feb 15.

Advanced Drug Delivery Laboratory, Department of Pharmaceutical Sciences, College of Pharmacy, Northeast Ohio Medical University, 4209 State Route 44, Rootstown, Ohio, 44272, USA.

The process of developing an old drug for new indications is now a widely accepted strategy of shortening drug development time, reducing drug costs, and improving drug availability, especially for rare and neglected diseases. In this mini-review, we highlighted the impact of drug delivery systems in the fulfillment of crucial aspects of drug repurposing such as (i) maximizing the repurposed drug effects on a new target, (ii) minimizing off-target effects, (iii) modulating the release profiles of drug at the site of absorption, (iv) modulating the pharmacokinetics/in vivo biodistribution of the repurposed drug, (v) targeting/modulating drug retention at the sites of action, and (vi) providing a suitable platform for therapeutic application of combination drugs. Read More

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http://dx.doi.org/10.1208/s12249-019-1333-zDOI Listing
February 2019

Private information conflict: Lasius niger ants prefer olfactory cues to route memory.

Anim Cogn 2019 Feb 15. Epub 2019 Feb 15.

Animal Comparative Economics Laboratory, Department of Zoology and Evolutionary Biology, University of Regensburg, Regensburg, Germany.

Foraging animals use a variety of information sources to navigate, such as memorised views or odours associated with a goal. Animals frequently use different information sources concurrently, to increase navigation accuracy or reliability. While much research has focussed on conflicts between individually learned (private) information and social information, conflicts between private information sources have been less broadly studied. Read More

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http://dx.doi.org/10.1007/s10071-019-01248-3DOI Listing
February 2019

First-in-class positron emission tomography tracer for the glucagon receptor.

EJNMMI Res 2019 Feb 15;9(1):17. Epub 2019 Feb 15.

Antaros Medical AB, Uppsala Science Park, Dag Hammarskjölds Väg 14B, Mölndal, SE-751 83, Uppsala, Sweden.

The glucagon receptor (GCGR) is emerging as an important target in anti-diabetic therapy, especially as part of the pharmacology of dual glucagon-like peptide-1/glucagon (GLP-1/GCG) receptor agonists. However, currently, there are no suitable biomarkers that reliably demonstrate GCG receptor target engagement.

Methods: Two potent GCG receptor peptide agonists, S01-GCG and S02-GCG, were labeled with positron emission tomography (PET) radionuclide gallium-68. Read More

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http://dx.doi.org/10.1186/s13550-019-0482-0DOI Listing
February 2019

Cerebrospinal fluid biomarkers for understanding multiple aspects of Alzheimer's disease pathogenesis.

Cell Mol Life Sci 2019 Feb 15. Epub 2019 Feb 15.

Centre of Excellence in Alzheimer's Disease Research and Care, School of Medical and Health Sciences, Edith Cowan University, 270 Joondalup Drive, Joondalup, WA, Australia.

Alzheimer's disease (AD) is a multifactorial age-related brain disease. Numerous pathological events run forth in the brain leading to AD. There is an initial long, dormant phase before the clinical symptoms become evident. Read More

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http://dx.doi.org/10.1007/s00018-019-03040-5DOI Listing
February 2019
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[Comments on the guidelines (2018) of the European Society of Cardiology (ESC) and the European Society of Hypertension (ESH) on the management of arterial hypertension].

Internist (Berl) 2019 Feb 15. Epub 2019 Feb 15.

Institut für Klinische Pharmakologie und Toxikologie, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, und Berlin Institute of Health, Charitéplatz 1, Berlin, 10117, Deutschland.

Arterial hypertension represents one of the most frequent chronic diseases that can lead to complications, such as stroke, dementia, heart attack, heart failure and renal failure. By 2025 the number of hypertensive patients will increase to approximately 1.6 billion people worldwide. Read More

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http://dx.doi.org/10.1007/s00108-019-0576-9DOI Listing
February 2019

Cost-effective and robust genotyping using double-mismatch allele-specific quantitative PCR.

Sci Rep 2019 Feb 15;9(1):2150. Epub 2019 Feb 15.

Center for Medical Genetics Ghent, Ghent University, Ghent, 9000, Belgium.

For a wide range of diseases, SNPs in the genome are the underlying mechanism of dysfunction. Therefore, targeted detection of these variations is of high importance for early diagnosis and (familial) screenings. While allele-specific PCR has been around for many years, its adoption for SNP genotyping or somatic mutation detection has been hampered by its low discriminating power and high costs. Read More

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http://dx.doi.org/10.1038/s41598-019-38581-zDOI Listing
February 2019

Multimaterial actinic spatial control 3D and 4D printing.

Nat Commun 2019 Feb 15;10(1):791. Epub 2019 Feb 15.

Department of Chemistry, University of Washington, Seattle, WA, 98195, USA.

Production of objects with varied mechanical properties is challenging for current manufacturing methods. Additive manufacturing could make these multimaterial objects possible, but methods able to achieve multimaterial control along all three axes of printing are limited. Here we report a multi-wavelength method of vat photopolymerization that provides chemoselective wavelength-control over material composition utilizing multimaterial actinic spatial control (MASC) during additive manufacturing. Read More

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http://dx.doi.org/10.1038/s41467-019-08639-7DOI Listing
February 2019

Rare gut microbiota associated with breeding success, hormone metabolites and ovarian cycle phase in the critically endangered eastern black rhino.

Microbiome 2019 Feb 15;7(1):27. Epub 2019 Feb 15.

School of Earth and Environmental Sciences, University of Manchester, Manchester, UK.

Background: Host microbiomes play a role in hormone production and subsequent fertility in humans, but this is less well understood in non-model organisms. This is of particular relevance to species in zoo-based conservation breeding programmes, as relationships between host microbiome composition and reproductive output may allow for the development of microbial augmentation strategies to improve success. Here, we characterise faecal bacterial communities of breeding and non-breeding eastern black rhino (Diceros bicornis michaeli) using 16S rRNA gene amplicon sequencing and quantify progestagen and glucocorticoid metabolite concentrations through enzyme immunoassays to identify such relationships. Read More

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http://dx.doi.org/10.1186/s40168-019-0639-0DOI Listing
February 2019

Repeat hepatectomy with systemic chemotherapy might improve survival of recurrent liver metastasis from colorectal cancer-a retrospective observational study.

World J Surg Oncol 2019 Feb 15;17(1):33. Epub 2019 Feb 15.

Department of Surgery Fujita Health University, 1-98 Dengakugakubo Kutsukake-cho, Toyoake City, Aichi, 470-1192, Japan.

Background: Although hepatectomy for metastatic colorectal cancer (mCRC) prolongs survival in up to 40% of people, recurrence rates approach 70%. We used a multidisciplinary approach to treat recurrent liver metastases, including chemotherapy, surgery, and palliative care. On the other hand, development of chemotherapeutic agents is remarkable and improves long-term survival. Read More

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http://dx.doi.org/10.1186/s12957-019-1575-yDOI Listing
February 2019

A new prokaryotic expression vector for the expression of antimicrobial peptide abaecin using SUMO fusion tag.

BMC Biotechnol 2019 Feb 15;19(1):13. Epub 2019 Feb 15.

MicroSynbiotiX Ltd, 11011 N Torrey Pines Rd Ste. #135, La Jolla, CA, 92037, USA.

Background: Despite the growing demand for antimicrobial peptides (AMPs) for clinical use as an alternative approach against antibiotic-resistant bacteria, the manufacture of AMPs relies on expensive, small-scale chemical methods. The small ubiquitin-related modifier (SUMO) tag is industrially practical for increasing the yield of recombinant proteins by increasing solubility and preventing degradation in expression systems.

Results: A new vector system, pKSEC1, was designed to produce AMPs, which can work in prokaryotic systems such as Escherichia coli and plant chloroplasts. Read More

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http://dx.doi.org/10.1186/s12896-019-0506-xDOI Listing
February 2019

Acupuncture as an Adjunctive Treatment for Angina Due to Coronary Artery Disease: A Meta-Analysis.

Med Sci Monit 2019 Feb 16;25:1263-1274. Epub 2019 Feb 16.

Department of Gynecology, The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing, China (mainland).

BACKGROUND In traditional Chinese medicine, acupuncture has been used to treat angina due to coronary artery disease (CAD). The aim of this systematic review of the literature and meta-analysis was to identify published randomized controlled trials (RCTs) that quantified the effectiveness of adjunctive acupuncture treatment in patients with angina due to CAD who were also treated with Western or Chinese medicine. MATERIAL AND METHODS A systematic review of the literature included a search of the PubMed, Embase, Cochrane library, and China National Knowledge Infrastructure (CNKI) databases, from their inception to September 2018. Read More

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http://dx.doi.org/10.12659/MSM.913379DOI Listing
February 2019

Micron resolution, high-fidelity three-dimensional vascular optical imaging phantoms.

J Biomed Opt 2019 Feb;24(2):1-4

Wellcome Trust-EPSRC Centre for Interventional and Surgical Sciences, London, United Kingdom.

Microscopic and mesoscale optical imaging techniques allow for three-dimensional (3-D) imaging of biological tissue across millimeter-scale regions, and imaging phantom models are invaluable for system characterization and clinical training. Phantom models that replicate complex 3-D geometries with both structural and molecular contrast, with resolution and lateral dimensions equivalent to those of imaging techniques (<20  μm), have proven elusive. We present a method for fabricating phantom models using a combination of two-photon polymerization (2PP) to print scaffolds, and microinjection of tailored tissue-mimicking materials to simulate healthy and diseased tissue. Read More

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http://dx.doi.org/10.1117/1.JBO.24.2.020502DOI Listing
February 2019

Non-destructive detection of matrix stabilization correlates with enhanced mechanical properties of self-assembled articular cartilage.

J Tissue Eng Regen Med 2019 Feb 15. Epub 2019 Feb 15.

Department of Biomedical Engineering, University of California Irvine, Irvine, CA, USA.

Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents. A non-destructive solution to monitor tissue quality and maturation would greatly reduce costs and accelerate the development of tissue engineered products. The objectives of this study were to 1) determine whether matrix stabilization with exogenous lysyl oxidase like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, 2) evaluate whether label-free, non-destructive, Fluorescence Lifetime Imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, 3) form quantitative relationships between destructive and nondestructive measurements to determine whether the strength of these correlations are sufficient to replace destructive testing methods, and 4) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking. Read More

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http://dx.doi.org/10.1002/term.2824DOI Listing
February 2019

Low-intensity pulsed ultrasound combination with iPSCs-NCSCs and GDF5 promotes sciatic nerve regeneration and functional recovery.

J Tissue Eng Regen Med 2019 Feb 15. Epub 2019 Feb 15.

Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, P. R. China.

The treatment of lengthy peripheral nerve defect is challenging in the field of nerve regeneration. Our previous studies have shown that low-intensity pulsed ultrasound (LIPUS) could promote the proliferation, cell viability, and neural differentiation of induced pluripotent stem cells-derived neural crest stem cells (iPSCs-NCSCs) and improve the regeneration of damaged peripheral nerve. In this study, the mechanical signal transduction pathway of LIPUS promoting iPSCs-NCSCs proliferation and differentiation was further explored, and the effects of LIPUS combined with iPSCs-NCSCs, perfluorotributylamine (PFTBA), and growth differentiation factor 5 (GDF5) on the repair of peripheral nerve injury were evaluated. Read More

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http://dx.doi.org/10.1002/term.2823DOI Listing
February 2019
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