168,656 results match your criteria Cold Spring Harbor protocols[Journal]
J Plant Physiol 2018 Dec 4;232:188-199. Epub 2018 Dec 4.
Department of Plants and Crops, Ghent University, Coupure links 653, 9000 Gent, Belgium. Electronic address:
We studied metabolic adaptations to cold stress in roses and identified genes in the carbohydrate pathway during acclimation and deacclimation. A field experiment with four rose cultivars belonging to different USDA plant hardiness zones was set up in Melle, Belgium (51° 0' N, 3° 48' E). The more cold-hardy cultivars ('Dagmar Hastrup' and 'John Cabot') reached their lowest LT value in December, indicating a rapid acclimation after the first occurrence of frost. Read More
J Clin Apher 2018 Dec 8. Epub 2018 Dec 8.
Department of Clinical Hematology - Blood Bank and Hemostasis, Onassis Cardiac Surgery Center, Kallithea, Greece.
Background: Perioperative therapeutic plasma exchange in patients with cardiovascular diseases poses several challenges, including alterations in intravascular volume, serum electrolytes, the coagulation cascade, and drug pharmacokinetics.
Methods: This review article summarizes different indications of plasma exchange for patients requiring cardiac surgery based on reported case reports and case series.
Results: The most common reported indication is plasma exchange for the management of allosensitized cardiac transplant candidate patients in combination with immunosuppressive regimens, which increases the likelihood of obtaining a cross-match-negative allograft, improving post-transplant clinical outcome. Read More
J Small Anim Pract 2018 Dec 11. Epub 2018 Dec 11.
Clinic for Small Animal Surgery and Reproduction, Ludwig-Maximilians-University, Munich 80539, Germany.
Objectives: To compare the efficacy of polyhexanide, cold argon plasma and saline in reducing bacterial bio-burden in dog bite wounds.
Materials And Methods: Prospective blinded randomised clinical trial. Dogs were randomly assigned to one of the treatment groups by lottery and bacterial cultures obtained before and after treatment were compared. Read More
Evolution 2018 Dec 11. Epub 2018 Dec 11.
Division of Ecology and Evolution, Research School of Biology, The Australian National University, 0200, Canberra, ACT, Australia.
Testing hypotheses on the drivers of clade evolution and trait diversification provides insight into many aspects of evolutionary biology. Often, studies investigate only the intrinsic biological properties of organisms as the causes of diversity, however extrinsic properties of a clade's environment, particularly geological history, may also offer compelling explanations. The Andes are a young mountain chain known to have shaped many aspects of climate and diversity of South America. Read More
Biotechnol Prog 2018 Dec 8:e2764. Epub 2018 Dec 8.
Department of Chemical Engineering, 1280 Main Street West, McMaster University, Hamilton, Ontario, Canada, L8S 4L7.
Rolling circle amplification (RCA), polymerase chain reaction (PCR), and loop-mediated isothermal amplification (LAMP), are powerful tools that can be used for gene manipulation, pathogen detection, and infectious disease diagnostics. However, these techniques require trained personnel, as the pipetting steps involved can lead to contamination and, consequently, erroneous results. Furthermore, many of the reagents used in molecular biology are thermally labile and must be kept within a cold-chain. Read More
Int Endod J 2018 Dec 8. Epub 2018 Dec 8.
Miadent Oral and Dental Health Center, Ankara, Turkey.
Aim: to compare the incidence, intensity and prediction of postoperative pain after glide path preparation with manual, continuous rotary and a novel reciprocating glide path instrument.
Methodology: The study included 240 patients, which were treated by 4 specialists according to a planned treatment protocol. Following access cavity preparation and prior to glide path preparation, the subjects were randomly assigned to 1 of the 3 groups according to the glide path instrument by choosing a sealed envelope containing the group name: R-Pilot (VDW, Munich, Germany), ProGlider (Dentsply Sirona, Ballaigues, Switzerland) and stainless-steel K-files (Dentsply Sirona) (n = 80). Read More
Eur J Cancer Care (Engl) 2018 Dec 10:e12976. Epub 2018 Dec 10.
Division of Hematology/Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Lin-Ko, Taiwan.
The purpose of this study was to evaluate the longitudinal incidence, severity, pattern of changes or predictors of oxaliplatin-induced peripheral neuropathy (OXAIPN) in Taiwanese patients with colorectal cancer. A longitudinal repeated measures study design was employed, and 77 participants were recruited from the colorectal and oncology departments of two teaching medical centres in Taiwan. Physical examinations were performed, and self-reports regarding adverse impacts of OXAIPN and quality of life were obtained at five time points throughout 12 cycles of chemotherapy (C/T). Read More
J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.
Histology and Cell Biology Department, Faculty of Medicine, Minia University, Minia, Egypt.
Omega-3 fatty acids are gaining attention as a therapeutic agent of many diseases. Their protective effect in a variety of diseases has been demonstrated. To the best of our knowledge, this is the first study on omega-3 fatty acids related to acute cold-restraint stress (CRS) induced hepatic dysfunction in rats. Read More
J Pharm Pharmacol 2018 Dec 10. Epub 2018 Dec 10.
Department of Pharmacology, Post Graduate Institute of Medical Education & Research (PGIMER), Chandigarh, India.
Objectives: The role of nuclear factor-2 erythroid related factor-2 (Nrf2) activator, berberine (BBR), has been established in rat model of streptozotocin induced diabetic neuropathy. Around 30-40% of cancer patients, on paclitaxel (PTX) chemotherapy develop peripheral neuropathy. The present study was contemplated with the aim of establishing the neuropathy preventive role of BBR, in paclitaxel induced peripheral neuropathy model in rats. Read More
Environ Sci Pollut Res Int 2018 Dec 8. Epub 2018 Dec 8.
Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, 250100, PR China.
In the present study, the performance of surface flow constructed wetlands (SFCWs) added with different dosage of biochar (group A 0%, group B 10%, group C 20%; v/v) was investigated, to evaluate the effect of biochar on nitrogen removal of a constructed wetland. No significant difference was observed in NH-N removal among three groups even during different seasons. Labile organic carbon released from biochar distinctly enhanced denitrification process, which improved NO-N removal efficiency by 4. Read More
Cogn Affect Behav Neurosci 2018 Dec 7. Epub 2018 Dec 7.
Dipartimento di Psicologia, Università di Bologna, Bologna, Italy.
The ventromedial prefrontal cortex (vmPFC) is known to play a key role in reward processing and decision making. However, its relative contribution to affect-rich ("hot") and affect-poor ("cold") decisions is not fully understood. Damage to vmPFC is associated with impaired performance on laboratory tasks of decision making under ambiguity and risk. Read More
Mol Biol Evol 2018 Dec 7. Epub 2018 Dec 7.
Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, USA.
Meiotic recombination comprises crossovers and non-crossovers. Recombination, crossover in particular, shuffles mutations and impacts both the level of genetic polymorphism and the speed of adaptation. In many species, the recombination rate varies across the genome with hot- and cold-spots. Read More
Heliyon 2018 Dec 1;4(12):e00999. Epub 2018 Dec 1.
Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS-Institut Armand-Frappier, Institute of Nutraceutical and Functional Foods, 531 Boulevard des Prairies, Laval, Québec, H7V 1B7, Canada.
Some pathogens might develop favorable global adaptation in response to certain stress treatments resulting in enhanced virulence and/or resistance to a different stress. β-lactam resistance, as well as and genes involved in this resistance, were studied to evaluate their possible role in O157:H7 () radioresistance. adapted to 25, 15 or 7 μg/mL of kanamycin or carbenicillin, were produced and treated with sensitization (0. Read More
Transplant Direct 2018 Nov 23;4(11):e400. Epub 2018 Oct 23.
Division of Organ Transplantation, Department of Surgery, UMass Memorial Medical Center, University of Massachusetts, Worcester, MA.
Background: It remains controversial if arterial perfusion in addition to portal vein perfusion during machine preservation improves liver graft quality. Comparative studies using both techniques are lacking. We studied the impact of using single or dual machine perfusion of donation after circulatory death rat livers. Read More
Plast Reconstr Surg Glob Open 2018 Oct 3;6(10):e1839. Epub 2018 Oct 3.
Division of Plastic Surgery, University of Alberta, Edmonton, Alberta, Canada.
The nose plays a critical role in olfaction, air filtration and humidification, and facial aesthetics. Most nasal amputations result from animal bites, human bites, and lacerations from glass. Successful replantation yields the best aesthetic and functional outcomes and is preferred compared with multistage nasal reconstruction. Read More
Front Microbiol 2018 26;9:2824. Epub 2018 Nov 26.
Department of Food Science, Faculty of Agriculture and Food Sciences, Institute of Nutrition and Functional Foods, Université Laval, Québec City, QC, Canada.
The aim of this study was to evaluate the impact of chitosan film, with bacteriocin divergicin 35 incorporate, on growth of in Cold smoked salmon. The simples of Cold-smoked wild salmon were inoculated with and treated with chitosan (100 kDa, 94.7% de-acetylated) and divergicin M35 was stored for 3 weeks at 4-8°C. Read More
Proteome Sci 2018 24;16:20. Epub 2018 Nov 24.
1Department of Bioresource Engineering, Macdonald Campus, McGill University, 21,111 Lakeshore Boulevard, Sainte-Anne-de-Bellevue, Quebec H9X 3V9 Canada.
Background: Abiotic stress reduces photosynthetic yield and plant growth, negatively impacting global crop production and is a major constraint faced by agriculture. However, the knowledge on the impact on plants under extremely high irradiance is limited. We present the first in-depth proteomics analysis of plants treated with a method developed by our research group to generate a light gradient using an extremely intense light. Read More
Trauma Case Rep 2018 Dec 27;18:42-45. Epub 2018 Nov 27.
Department of Orthopaedic and Traumatology, Penang General Hospital, Georgetown, Malaysia.
Open traumatic scapulothoracic dissociation is a rare and devastating injury. We are reporting a 21-year-old male factory worker who sustained a traumatic open scapulothoracic dissociation. His left arm was caught in conveyer belts resulting the arm, scapula, clavicle, and pectoral muscle torn from the body by tremendous traction force. Read More
J Transplant 2018 8;2018:9205083. Epub 2018 Nov 8.
Montreal Heart Institute, Université de Montréal, Montreal, QC, Canada.
Background: Recent changes in the demographic of cardiac donors and recipients have modulated the rate and risk, associated with posttransplant diabetes mellitus (PTDM). We investigated the secular trends of the risk of PTDM at 1 year and 3 years after transplantation over 30 years and explored its effect on major outcomes.
Methods: Three hundred and three nondiabetic patients were followed for a minimum of 36 months, after a first cardiac transplantation performed between 1983 and 2011. Read More
PLoS One 2018 11;13(12):e0208721. Epub 2018 Dec 11.
Institute for Materials Discovery, University College London, London, United Kingdom.
The present work investigates the process of frosting formation on arched copper samples with different surface temperatures, calculated the thickness of the frost layer by using the scale method, and analyzed frost lodging, melting, and other phenomena that appeared during the frost-formation process. The results showed that the frosting process on an arched surface can be divided into ice-film formation, rapid growth of the frost layer, and stable growth of the frost layer. Meanwhile, the phenomena of frost-branch breakage, lodging, and melting were observed. Read More
PLoS One 2018 11;13(12):e0202375. Epub 2018 Dec 11.
Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi'an, China.
Circular RNAs (circRNAs) are a class of endogenous noncoding RNAs, which increasingly drawn researchers' attention in recent years as their importance in regulating gene expression at the transcriptional and post-transcriptional levels. With the development of high-throughput sequencing and bioinformatics, circRNAs have been widely analysed in animals, but the understanding of characteristics and function of circRNAs is limited in plants, especially in maize. Here, 3715 unique circRNAs were predicted in Zea mays systematically, and 8 of 12 circRNAs were validated by experiments. Read More
Front Microbiol 2018 23;9:2873. Epub 2018 Nov 23.
Geomicrobiology, GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany.
Deep-sea hydrothermal vents may provide one of the largest reservoirs on Earth for hydrogen-oxidizing microorganisms. Depending on the type of geological setting, hydrothermal environments can be considerably enriched in hydrogen (up to millimolar concentrations). As hot, reduced hydrothermal fluids ascend to the seafloor they mix with entrained cold, oxygenated seawater, forming thermal and chemical gradients along their fluid pathways. Read More
J Vis Exp 2018 Nov 20(141). Epub 2018 Nov 20.
Institute of Vegetables, Zhejiang Academy of Agricultural Sciences; State Key Lab Breeding Base for Sustainable Control of Plant Pest and Disease, Zhejiang Academy of Agricultural Sciences;
MicroRNAs (miRNAs) are endogenous small non-coding RNAs of about 20 - 24 nt, known to play important roles in plant development and adaptation. There is an accumulating evidence showing that the expressions of certain miRNAs are altered when grafting, an agricultural practice commonly used by farmers to improve crop tolerance to biotic and abiotic stresses. Bottle gourd is an inherently climate-resilient crop compared to many other major cucurbits, including watermelon, rendering it one of the most widely used rootstocks for the latter. Read More
Med Sci Sports Exerc 2018 Dec 5. Epub 2018 Dec 5.
Department of Kinesiology, Kansas State University, Manhattan, KS.
Introduction: the aim of the current investigation was to examine if dietary nitrate supplementation would improve vascular control in hypertensive post-menopausal women (PMW). We tested the hypotheses that acute dietary nitrate supplementation would 1) significantly decrease arterial blood pressure (BP) at rest and during exercise; 2) increase limb blood flow during steady-state exercise; and 3) improve functional sympatholysis during steady-state (SS) exercise.
Methods: ten hypertensive PMW underwent a randomized, double-blind placebo-controlled trial with a nitrate-rich (NR) or nitrate poor (NP) supplement. Read More
Int J Rehabil Res 2018 Dec 10. Epub 2018 Dec 10.
Istituti Clinici Scientifici Maugeri, IRCCS, Bioengineering Unit of Veruno Institute (NO) Italy.
The aim of this study was to analyse the Cold Intolerance Symptom Severity (CISS) questionnaire in its Italian validated version, using Rasch analysis, to gain insights for a possible refinement of the questionnaire. The CISS was administered to a convenience sample of 96 consecutively recruited outpatients with upper limb peripheral nerve injury. Data were analysed using Rasch analysis. Read More
Exp Anim 2018 Dec 10. Epub 2018 Dec 10.
School of Energy and Environment Engineering, Zhongyuan University of Technology.
Inhalation of pathogenic bacteria transported by particulate matter (PM) presents an important potential threat to human health. Therefore, the pulmonary toxicity in mice caused by Staphylococcus aureus (S. aureus) and PM as individual matter and mixtures was studied. Read More
Scand J Pain 2018 Dec 7. Epub 2018 Dec 7.
School of Psychology, Keele University, Keele, Staffordshire, UK.
Background and aims Prior research indicates that swearing increases pain tolerance and decreases pain perception in a cold pressor task. In two experiments, we extend this research by testing whether taboo hand gesticulations have a similar effect. Methods Study 1 focused on males and females who, across two trials, submerged an extended middle finger (taboo) and an extended index finger (control) in ice water until discomfort necessitated removal. Read More
J Endocrinol 2019 Feb;240(2):257-269
Naomi Berrie Diabetes Center, Department of Pathology and Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University, New York, New York, USA.
Fat remodeling has been extensively explored through protein deacetylation, but not yet acetylation, as a viable therapeutic approach in the management of obesity and related metabolic disorders. Here, we investigated the functions of key acetyltransferases CBP/p300 in adipose remodeling and their physiological effects by generating adipose-specific deletion of CBP (Cbp-AKO), p300 (p300-AKO) and double-knockout (Cbp/p300-AKO) models. We demonstrated that Cbp-AKO exhibited marked brown remodeling of inguinal WAT (iWAT) but not epididymal WAT (eWAT) after cold exposure and that this pattern was exaggerated in diet-induced obesity (DIO). Read More
J Nucl Med 2018 Dec 7. Epub 2018 Dec 7.
Children's Hospital Boston, United States.
The purpose of this study was to assess image quality and quantitative brain PET across a multi-center consortium. All academic centers and children's hospitals in the Pediatric Brain Tumor Consortium (PBTC) scanned a phantom developed by the Society of Nuclear Medicine and Molecular Imaging Clinical Trials Network (SNMMI CTN) for the validation of brain PET studies associated with clinical trials. The phantom comprises 2 separate, fillable sections: a resolution/uniformity section and a clinical simulation section. Read More
Proc Natl Acad Sci U S A 2018 Dec 10. Epub 2018 Dec 10.
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907;
Enterovirus D68 (EV-D68) belongs to a group of enteroviruses that contain a single positive-sense RNA genome surrounded by an icosahedral capsid. Like common cold viruses, EV-D68 mainly causes respiratory infections and is acid-labile. The molecular mechanism by which the acid-sensitive EV-D68 virions uncoat and deliver their genome into a host cell is unknown. Read More
Int J Food Microbiol 2018 Nov 29;291:181-188. Epub 2018 Nov 29.
French Agency for Food, Environmental and Occupational Health & Safety (Anses), Laboratory for Food Safety, Université Paris-Est, Maisons-Alfort F-94701, France. Electronic address:
Intraspecific variability of the behavior of most foodborne pathogens is well described and taken into account in Quantitative Microbial Risk Assessment (QMRA), but factors (strain origin, serotype, …) explaining these differences are scarce or contradictory between studies. Nowadays, Whole Genome Sequencing (WGS) offers new opportunities to explain intraspecific variability of food pathogens, based on various recently published bioinformatics tools. The objective of this study is to get a better insight into different existing bioinformatics approaches to associate bacterial phenotype(s) and genotype(s). Read More
Biomed Pharmacother 2018 Dec 5;110:409-419. Epub 2018 Dec 5.
Agricultural Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig, 44519, Egypt. Electronic address:
Sexual dysfunction in the epileptic patient is difficult to confirm whether it is ailment or therapy related. Antiepileptic drugs often use in reproductive age, through reproductive progress and maturation. On the other side, cold-pressed oils are rich in bioactive phytochemicals with health-promoting traits. Read More
Ecotoxicol Environ Saf 2018 Dec 6;170:253-258. Epub 2018 Dec 6.
Key Laboratory of Environment and Health (HUST), Ministry of Education & Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, Hubei, China; State Key Laboratory of Environmental Health (Incubation), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, Hubei, China. Electronic address:
Prenatal life exposure to fine particulate matter (aerodynamic diameter less than or equal to 2.5 µm, PM) has been linked with increased risk of adverse fetal development and birth outcomes in previous studies. However, to our knowledge, no study has investigated the association of maternal PM with the risk of fetal distress, which is a harmful fetal status and may lead to fetal brain damage, even fetal death. Read More
Genomics 2018 Dec 6. Epub 2018 Dec 6.
School of Computer Science, Xidian University, Xi'an, 710071, PR China.
Meiotic recombination plays an important role in the process of genetic evolution. Previous researches have shown that the recombination rates provide important information about the mechanism of recombination study. However, at present, most methods ignore the hidden correlation and spatial autocorrelation of the DNA sequence. Read More
Int J Biol Macromol 2018 Dec 4. Epub 2018 Dec 4.
Department of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:
In this study, the influence of Persian gum (PG) on the properties of whey protein concentrate (WPC) emulsion gel prepared through cold set gelation method (incorporation of CaCl) was investigated. The mean droplet size of emulsions was analyzed prior to gelation and the emulsion gel samples were characterized by rheological studies, scanning electron microscopy (SEM) and water holding capacity (WHC) measurement. Results showed PG affected the droplet size of the initial emulsions and the matrix of gel systems. Read More
Int J Biol Macromol 2018 Dec 4. Epub 2018 Dec 4.
Natural Products & Green Chemistry Division, Central Salt & Marine Chemicals Research Institute, G. B Marg, Bhavnagar 364002, Gujarat, India; Academy of Scientific and Innovative Research (AcSIR), Central Salt & Marine Chemicals Research Institute, G. B Marg, Bhavnagar 364002, Gujarat, India. Electronic address:
Herein, we demonstrate the preparation of superabsorbent hydrogel (CMA-g-PAm) materials using anionic and cold water soluble carboxymethyagarose (CMA, a seaweed polysaccharide-based derivative) with polyacrylamide (PAm) through rapid microwave assisted grafting technique. The successful fabrication of CMA-g-PAm was verified by FT-IR, SEM, XRD, TGA and CHN analyzer. Effects of initiator, crosslinker, and monomer on the swelling behavior and grafting parameters have been thoroughly investigated. Read More
Chemosphere 2018 Dec 1;219:15-27. Epub 2018 Dec 1.
Department of Pharmacology, Federal University of Paraná, P.O. Box 19031, 81530-980, Curitiba, Paraná, Brazil. Electronic address:
This study combined data of the concentrations of metals, polycyclic aromatic hydrocarbons (PAHs) and pharmaceuticals and personal hygiene products (PPCPs) in the sediments and the biological responses of the Atherinella brasiliensis fish in two different sites and climate seasons in the Estuarine-Lagoon Complex of Iguape-Cananéia, Southeast Brazil. The presence of metals, PAHs, and PPCPs were observed in the sediments demonstrating the contamination throughout the system with contributions of sewage and residues disposal, oil and combustion of biomass and fossil fuels. Higher contaminations were identified in the point of greater human presence (C - Cananéia City), especially during the cold-dry season. Read More
Neuropharmacology 2018 Dec 5. Epub 2018 Dec 5.
pharmazentrum frankfurt/ZAFES, Institut für Klinische Pharmakologie, Klinikum der Goethe-Universität Frankfurt, Theodor Stern Kai 7, 60590, Frankfurt am Main, Germany. Electronic address:
Inhibitor-kappaB kinase epsilon (IKKε, Ikbke) constitutes an NF-κB activating kinase with high homology to the classical I-κB kinase subunits, IKKα and IKKβ. It is expressed in nociceptive neurons in the spinal cord and in dorsal root ganglia (DRG) and involved in inflammatory nociception. Under inflammatory conditions, IKKε deficient mice show significantly less nociceptive behavior in comparison to wild type mice associated with reduced activation of NF-κB and attenuated NF-κB-dependent gene expression. Read More
J Environ Sci (China) 2019 Feb 7;76:339-348. Epub 2018 Jun 7.
Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, Kumamoto 862-8502, Japan. Electronic address:
Traffic vehicles, many of which are powered by port fuel injection (PFI) engines, are major sources of particulate matter in the urban atmosphere. We studied particles from the emission of a commercial PFI-engine vehicle when it was running under the states of cold start, hot start, hot stabilized running, idle and acceleration, using a transmission electron microscope and an energy-dispersive X-ray detector. Results showed that the particles were mainly composed of organic, soot, and Ca-rich particles, with a small amount of S-rich and metal-containing particles, and displayed a unimodal size distribution with the peak at 600 nm. Read More
J Surg Res 2019 Feb 27;234:7-12. Epub 2018 Sep 27.
Division for Endocrine Surgery, Department of Human Pathology in Adulthood and Childhood "G. Barresi," University Hospital G. Martino, University of Messina, Messina, Italy.
Background: Recurrent laryngeal nerve (RLN) injury is the most common and serious complication after thyroid surgery. However, little is known about the temperature threshold leading to RLN injury. In this study, we investigated threshold temperatures that cause RLN injury during thyroid surgery using continuous intraoperative neuromonitoring in swine models. Read More
Elife 2018 Dec 11;7. Epub 2018 Dec 11.
Cold Spring Harbor Laboratory, Cold Spring Harbor, United States.
Successful treatment decisions in cancer depend on the accurate assessment of patient risk. To improve our understanding of the molecular alterations that underlie deadly malignancies, we analyzed the genomic profiles of 17,879 tumors from patients with known outcomes. We find that mutations in almost all cancer driver genes contain remarkably little information on patient prognosis. Read More
Biol Res 2018 Dec 8;51(1):55. Epub 2018 Dec 8.
Fundación Científica y Cultural Biociencia, José Domingo Cañas 2280, Santiago, 7750132, Chile.
Background: The Antarctic continent is a source of extreme microorganisms. Millions of years of isolation have produced unique biodiversity with adaptive responses to its extreme environment. Although the Antarctic climate is mainly cold, the presence of several geothermal sites, including thermal springs, fumaroles, hot soils and hydrothermal vents, provides ideal environments for the development of thermophilic and hyperthermophilic microorganisms. Read More
Genome Biol 2018 Dec 7;19(1):215. Epub 2018 Dec 7.
The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK.
Background: DNA is subject to constant chemical modification and damage, which eventually results in variable mutation rates throughout the genome. Although detailed molecular mechanisms of DNA damage and repair are well understood, damage impact and execution of repair across a genome remain poorly defined.
Results: To bridge the gap between our understanding of DNA repair and mutation distributions, we developed a novel method, AP-seq, capable of mapping apurinic sites and 8-oxo-7,8-dihydroguanine bases at approximately 250-bp resolution on a genome-wide scale. Read More
BMC Infect Dis 2018 Dec 7;18(1):638. Epub 2018 Dec 7.
China National Center for Food Safety Risk Assessment, Beijing, 100022, China.
Background: Hand, foot and mouth disease (HFMD) has become a substantial threat recently. However few studies have quantified spatiotemporal heterogeneity of HFMD and detected spatiotemporal interactive effect of potential driving factors on this disease.
Methods: Using GeoDetector and Bayesian space-time hierarchy model, we characterized the epidemiology of HFMD in Henan, one of the largest population provinces in China, from 2012 to 2013, and quantified the impacts of potential driving factors. Read More
Epigenetics 2018 Dec 11. Epub 2018 Dec 11.
a Centre for Sustainable Aquatic Research, College of Science, Swansea University , Swansea , SA2 8PP , UK.
Stress experienced during early life may have lasting effects on the immune system, with impacts on health and disease dependent on the nature and duration of the stressor. The epigenome is especially sensitive to environmental stimuli during early life and represents a potential mechanism through which stress may cause long-lasting health effects. However, the extent to which the epigenome responds differently to chronic vs acute stressors is unclear, especially for non-mammalian species. Read More
Foodborne Pathog Dis 2018 Dec 11. Epub 2018 Dec 11.
3 Institute of Meat Hygiene and Technology , Belgrade, Serbia .
The aim of this study was to determine the effects of different temperatures and storage time on Staphylococcus aureus growth, sea gene expression, and synthesis of staphylococcal enterotoxin A (SEA) in the pasteurized and UHT-pasteurized milk. Pasteurized and UHT-pasteurized milk were inoculated with 3.98 log CFU/mL of S. Read More
Annu Rev Biomed Eng 2018 Dec 10. Epub 2018 Dec 10.
Center for Engineering in Medicine and BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA; email: , ,
Successful stabilization and preservation of biological materials often utilize low temperatures and dehydration to arrest molecular motion. Cryoprotectants are routinely employed to help the biological entities survive the physicochemical and mechanical stresses induced by cold or dryness. Molecular interactions between biomolecules, cryoprotectants, and water fundamentally determine the outcomes of preservation. Read More