45,462 results match your criteria Clostridial Gas Gangrene


Clostridium septicum: A usual suspect? Aortic rupture following right hemicolectomy: A case report.

Int J Surg Case Rep 2018 Nov 22;54:51-54. Epub 2018 Nov 22.

Department of Surgery, Epworth Health, Melbourne Australia; Gastrointestinal Clinical Institute, Epworth HealthCare, Melbourne Australia.

Introduction: There is a known association between Clostridium Septicum, mycotic aneurysm and colon neoplasm.

Case Presentation: We report the case of a 90 year old female admitted with abdominal pain to the general surgery unit. Admission CT scan demonstrated a thick walled caecum and pericaecal inflammation suspicious for a perforated carcinoma. Read More

View Article
November 2018
1 Read

Bezlotoxumab Use as Adjunctive Therapy with the Third Fecal Microbiota Transplant in Refractory Recurrent Clostridium Difficile Colitis; A Case Report and Concise Literature Review.

Anaerobe 2018 Dec 3. Epub 2018 Dec 3.

ABIM Board Certified in Focused Practice in Hospital Medicine (FPHM), Mercy Clinic Hospitalist, Physician Building, 7301 Rogers Ave, Fort Smith, AR, 72903, United States. Electronic address:

Clostridium difficile is the most commonly reported pathogen to cause nosocomial infections in the United States with a high burden affecting morbidity, mortality and healthcare expenditure. The use of Fecal Microbiota Transplantation (FMT) is one of the current standard therapies for recurrent C. difficile infection (CDIr). Read More

View Article
December 2018

Gut microbiota signatures in cystic fibrosis: Loss of host CFTR function drives the microbiota enterophenotype.

PLoS One 2018 6;13(12):e0208171. Epub 2018 Dec 6.

Unit of Human Microbiome, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.

Background: Cystic fibrosis (CF) is a disorder affecting the respiratory, digestive, reproductive systems and sweat glands. This lethal hereditary disease has known or suspected links to the dysbiosis gut microbiota. High-throughput meta-omics-based approaches may assist in unveiling this complex network of symbiosis modifications. Read More

View Article
December 2018
1 Read

[Foodbourne Botulism: A Forgotten Disease].

Acta Med Port 2018 Nov 30;31(11):691-693. Epub 2018 Nov 30.

Serviço de Medicina Interna. Hospital Sousa Martins. Guarda. Portugal.

Botulism is a serious illness caused by exposure to botulinum toxin. It is manifested by flaccid, paralysis, symmetric and in descending pattern affecting cranial and peripheral nerves. Given the frequent need for invasive mechanical ventilation, these patients should be approached in an intensive care setting. Read More

View Article
November 2018

Radical SAM-dependent adenosylation catalyzed by l-tyrosine lyases.

Org Biomol Chem 2018 Dec 6. Epub 2018 Dec 6.

Department of Chemistry, Fudan University, Shanghai 200433, China.

The radical S-adenosylmethionine (SAM) superfamily is currently the largest known enzyme family. These enzymes reductively cleave SAM to produce a highly reactive 5'-deoxyadenosyl (dAdo) radical, which abstracts a hydrogen from the substrate and initiates diverse reactions. The canonic dAdo radical-mediated hydrogen abstraction can be changed to radical addition reactions by using olefin-containing substrate analogues, which result in adenosylation reactions. Read More

View Article
December 2018
1 Read

Linkages between epithelial microbiota and host transcriptome in the ileum during high grain challenges: implications for gut homeostasis in goats.

J Agric Food Chem 2018 Dec 6. Epub 2018 Dec 6.

A high-grain diet (HG) can result in ruminal subacute acidosis, which is detrimental to gut health and can lead to decreased productivity. This study investigated the ileal epithelial microbiota and its relationship with host epithelial function in goats fed an HG diet (concentrate: hay, 90: 10) and a Control diet (concentrate: hay, 55: 45) aiming to elucidate the mechanisms involved in ileal adaptation to subacute acidosis. The HG challenge increased ileal volatile fatty acid concentration (P = 0. Read More

View Article
December 2018
1 Read

Fecal Microbiota Transplantation Capsules with Targeted Colonic Versus Gastric Delivery in Recurrent Clostridium difficile Infection: A Comparative Cohort Analysis of High and Lose Dose.

Dig Dis Sci 2018 Dec 5. Epub 2018 Dec 5.

Finch Therapeutics, Somerville, MA, USA.

Background: Fecal microbiota transplantation (FMT) is an effective therapy for recurrent Clostridium. difficile infection (rCDI). FMT capsules have emerged, and it is unknown if delivery location and dose impact efficacy. Read More

View Article
December 2018
1 Read

Increased sensitivity of microbiological testing of cornea organ culture medium by additional resin treatment.

BMJ Open Ophthalmol 2018 10;3(1):e000173. Epub 2018 Nov 10.

Institute for Transfusion Medicine, University Tissue Bank, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Objective: This validation study investigates the treatment of cornea organ culture medium (Modified Eagle Medium, Biochrom GmbH, Berlin, Germany) with RESEP, a new medical device for antibiotics removal, before microbiological testing with BACTEC blood culture bottles.

Methods And Analysis: 10-100 colony forming units of and were inoculated in 9mL of cornea organ culture medium. In group A, the medium was withdrawn with RESEP and treated for 20 min at room temperature, and then inoculated in BACTEC Plus Aerobic/F/Anaerobic/F blood culture bottles. Read More

View Article
November 2018
2 Reads

Microbiota profile in new-onset pediatric Crohn's disease: data from a non-Western population.

Gut Pathog 2018 29;10:49. Epub 2018 Nov 29.

7Physics Department, Boston University, Boston, USA.

Background: The role of microbiota in Crohn's disease (CD) is increasingly recognized. However, most of the reports are from Western populations. Considering the possible variation from other populations, the aim of this study was to describe the microbiota profile in children with CD in Saudi Arabia, a non-Western developing country population. Read More

View Article
November 2018
1 Read

The antimicrobial peptide CopA3 inhibits toxin A-induced viability loss and apoptosis in neural cells.

J Microbiol Biotechnol 2018 Nov 16. Epub 2018 Nov 16.

Divison of Life Science and Chemistry, College of Natural Science, Daejin University, Pocheon, Gyeonggido, South Korea.

Numerous studies have reported that enteric neurons involved in controlling neurotransmitter secretion and motility in the gut critically contribute to the progression of gut inflammation. toxins, which cause severe colonic inflammation, are also known to affect enteric neurons. Our previous study showed that toxin A directly induces neural cell toxicities, such as viability loss and apoptosis. Read More

View Article
November 2018

Convergence of Bigelow and Arrhenius models over a wide range of heating temperatures.

Int J Food Microbiol 2018 Nov 20;291:173-180. Epub 2018 Nov 20.

Université de Brest, EA3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, UMT14.01 SporeRisk, 6 rue de l'Université, F-29334 Quimper, France.

The heat resistance of the bacterial spores of Moorella thermoacetica, Clostridium sporogenes, Geobacillus stearothermophilus and Bacillus coagulans was determined over a wide range of temperatures using the capillary method and thermoresistometer Mastia. The results showed that the two experimental methods gave similar heat resistance values excepted for Geobacillus stearothermophilus. The effect of temperature on thermal resistance was evaluated using the Arrhenius and Bigelow models. Read More

View Article
November 2018

Diversity and metabolism of xylose and glucose fermenting microbial communities in sequencing batch or continuous culturing.

FEMS Microbiol Ecol 2018 Dec 3. Epub 2018 Dec 3.

Delft University of technology, Department of Biotechnology, Van der Maasweg 9, 2629 HZ Delft, the Netherlands.

A mechanistic understanding of microbial community establishment and product formation in open fermentative systems can aid the development of bioprocesses utilising organic waste. Kinetically, a single rate-limiting substrate is expected to result in one dominant species. Four enrichment cultures were operated to ferment either xylose or glucose in a sequencing batch reactor (SBR) or a continuous-flow stirred tank reactor (CSTR) mode. Read More

View Article
December 2018

Pharmacologic Approach to Management of Clostridium difficile Infection.

Crit Care Nurs Q 2019 Jan/Mar;42(1):2-11

Department of Pharmacy, St Elizabeth Healthcare, Edgewood, Kentucky (Drs Martin, Vincent, Gillian, Moore, and Ratermann); Department of Pharmacy Services, University of Cincinnati Medical Center, and Division of Pharmacy Practice and Administrative Sciences, James L. Winkle College of Pharmacy, Cincinnati, Ohio (Dr Droege).

Clostridium difficile is a gram-positive, anaerobic, spore-forming bacterium that is the leading cause of nosocomial infections in hospitals in the United States. Critically ill patients are at high risk for C. difficile infection (CDI) and face potentially detrimental effects, including prolonged hospitalization, risk of recurrent disease, complicated surgery, and death. Read More

View Article
December 2018

Prevention of Clostridium difficile Infection in Critically Ill Adults.

Pharmacotherapy 2018 Dec 3. Epub 2018 Dec 3.

Department of Pharmacy, Mayo Clinic, Rochester, MN.

The incidence and severity of Clostridium difficile infection (CDI) remain high across intensive care units in the United States despite national efforts to decrease this escalating health care burden. Most published literature and guidelines address treatment rather than prevention, yet this approach may be too downstream to limit morbidity and mortality from the disease and its complications. Mechanisms to successfully prevent CDI include reducing modifiable risk factors and minimizing horizontal transmission of C. Read More

View Article
December 2018

Enhanced butanol production by optimization of medium parameters using YM1.

Saudi J Biol Sci 2018 Nov 15;25(7):1308-1321. Epub 2016 Feb 15.

School of Biosciences and Biotechnology, Faculty of Sciences and Technology, Universiti Kebangsaan Malaysia, 43600 UKM, Bangi, Selangor, Malaysia.

A new isolate of the solvent-producing YM1 was used to produce butanol in batch culture fermentation. The effects of glucose concentration, butyric acid addition and C/N ratio were studied conventionally (one-factor-at-a-time). Moreover, the interactions between glucose concentration, butyric acid addition and C/N ratio were further investigated to optimize butanol production using response surface methodology (RSM). Read More

View Article
November 2018
1 Read

Administration of antibiotics contributes to cholestasis in pediatric patients with intestinal failure via the alteration of FXR signaling.

Exp Mol Med 2018 Nov 30;50(12):155. Epub 2018 Nov 30.

Department of Pediatric Surgery, Xin Hua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

The link between antibiotic treatment and IF-associated liver disease (IFALD) is unclear. Here, we study the effect of antibiotic treatment on bile acid (BA) metabolism and investigate the involved mechanisms. The results showed that pediatric IF patients with cholestasis had a significantly lower abundance of BA-biotransforming bacteria than patients without cholestasis. Read More

View Article
November 2018

Microbial balance in the intestinal normal microbiome and its association with diabetes, obesity and allergic disease.

Microb Pathog 2018 Nov 29. Epub 2018 Nov 29.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

Recent studies have been considered to symbiotic interactions of the human gastrointestinal microbiota and human lifestyle-related disorders. The human gastrointestinal microbiota continuously stimulates the immune system against opportunistic and pathogen bacteria from infancy. Changes in gastrointestinal microbiota have been associated with numbers of human diseases such as allergic diseases, autoimmune encephalitis, atherosclerosis, colorectal cancer, obesity, diabetes etc. Read More

View Article
November 2018
1 Read
2.000 Impact Factor

Review of Management Options for Active-Phase Peyronie's Disease.

Sex Med Rev 2018 Nov 29. Epub 2018 Nov 29.

Department of Urology and Pediatric Urology, Julius-Maximilians-University of Würzburg, Würzburg, Germany.

Introduction: Peyronie's disease (PD) is a fibrotic disorder that leads to plaque formation in ∼3-9% of adult men. Connective tissue buildup results in plaque formation, penile curvature and shortening, and in some cases, pain. In the active phase of PD, penile pain is a common symptom, often accompanied by progression in curvature. Read More

View Article
November 2018

Botulinum toxin in women's health: An update.

Maturitas 2019 Jan 22;119:21-24. Epub 2018 Oct 22.

The Royal Women's Hospital, 20 Flemington Road Parkville, Victoria 3052 Australia; The University of Melbourne, Department of Obstetrics and Gynaecology, 7thFloor Royal Women's Hospital, 20 Flemington Road Parkville, Victoria 3052 Australia. Electronic address:

Botulinum toxin, from the bacteria Clostridium botulinum, acts by block the pre-synaptic release of acetylcholine at the neuromuscular junction, resulting in temporary muscle paralysis. There are several clinically available formulations, the most extensively studied being of onabotulinumtoxinA. This article outlines three of the common clinical applications of onabotulinumtoxinA relevant to women's health: the management of symptoms of overactive bladder, chronic migraine and facial lines. Read More

View Article
January 2019

A trial of pulsed xenon ultraviolet disinfection to reduce Clostridioides difficile infection.

Am J Infect Control 2018 Nov 27. Epub 2018 Nov 27.

Mayo Clinic, Rochester, MN.

Background: An intervention was designed to test whether the addition of an ultraviolet (UV) disinfection step after terminal cleaning would be helpful in reducing Clostridium difficile infection (CDI) rates in a real-world situation.

Methods: This study was a quasi-experimental design using 3 units as intervention units for the intervention and 3 similar units as control units. Intervention units 2 hematology and bone marrow transplant units and one medical-surgical unit at a large teaching hospital in the Midwest. Read More

View Article
November 2018

Diffusion network of CO in FeFe-Hydrogenase.

J Chem Phys 2018 Nov;149(20):204108

Department of Chemical Engineering, University of New Hampshire, 33 Academic Way, Durham, New Hampshire 03824, USA.

FeFe-hydrogenase is an efficient enzyme to produce H under optimal conditions. However, the activity of this enzyme is highly sensitive to the presence of inhibitory gases CO and O that cause irreversible damage to the active site. Therefore, a detailed knowledge of the diffusion pathways of these inhibitory gases is necessary to develop strategies for designing novel enzymes that are tolerant to these gases. Read More

View Article
November 2018

The Fatty Acid Synthesis Protein Enoyl-ACP Reductase II (FabK) is a Target for Narrow-Spectrum Antibacterials for Clostridium difficile Infection.

ACS Infect Dis 2018 Dec 1. Epub 2018 Dec 1.

Clostridium difficile infection (CDI) is an antibiotic-induced microbiota shift disease of the large bowel. While there is a need for narrow-spectrum CDI antibiotics, it is unclear which cellular proteins are appropriate drug targets to specifically inhibit C. difficile. Read More

View Article
December 2018

The predictive value of quantitative nucleic acid amplification detection of Clostridium difficile toxin gene for faecal sample toxin status and patient outcome.

PLoS One 2018 5;13(12):e0205941. Epub 2018 Dec 5.

Leeds Teaching Hospitals NHS Trust, and University of Leeds, Leeds, United Kingdom.

Background: Laboratory diagnosis of Clostridium difficile infection (CDI) remains unsettled, despite updated guidelines. We investigated the potential utility of quantitative data from a nucleic acid amplification test (NAAT) for C. difficile toxin gene (tg) for patient management. Read More

View Article
December 2018
1 Read

Modulation of intestinal microbiota, morphology and mucin composition by dietary insect meal inclusion in free-range chickens.

BMC Vet Res 2018 Dec 4;14(1):383. Epub 2018 Dec 4.

Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy.

Background: Gut health in poultry depends on the balance between the host, intestinal microbiota, intestinal microscopic features and diet. The effects of insect meal (a promising alternative protein source for poultry feed) on chicken gut morphology have recently been reported, but no data about intestinal microbiota and mucin composition modulation are available. The present study evaluated the effects of dietary Tenebrio molitor (TM) meal inclusion on gut health of free-range chickens by intestinal microbiota, morphology and mucin composition characterization. Read More

View Article
December 2018
1 Read

Exotic Tourist Destinations and Transmission of Infections by Swimming Pools and Hot Springs-A Literature Review.

Int J Environ Res Public Health 2018 Dec 3;15(12). Epub 2018 Dec 3.

Department of Biomedical Sciences, University of West Attica, 12243 Egaleo, Greece.

A growing number of people undertake international travel, and yet faster growth of such travel is expected in the tropics. Information on the hazards presented by pool and hot spring waters in tropical countries is very limited. This review aims to collate available information on pool water quality, alongside data on cases and outbreaks associated with swimming in pools in tropical regions affecting both local populations and travellers. Read More

View Article
December 2018

À la chirurgie s’ajoute désormais la possibilité d’un médicament par voie injectable.

Rev Prat 2017 Jun;67(6):628-631

Service d'urologieandrologie, CHU Carémeau, Nîmes, France.

Lapeyronie's disease. Lapeyronie's disease is a common condition responsible for an acquired penile curvature due to a fibrous albugineal plaque, affecting 3.4 to 9% of the male population. Read More

View Article

Among inpatients, ischemic bowel disease predisposes to Clostridium difficile infection with concomitant higher mortality and worse outcomes.

Eur J Gastroenterol Hepatol 2019 Jan;31(1):109-115

Department of Medicine, North Shore Medical Center, Salem.

Background And Aims: Clinical conditions resulting in hypoxia, hypoperfusion, anaerobic milieu within the gut, and intestinal epithelial breakdown, such as seen in heart failure, precipitates Clostridium difficile infection (CDI). Given that ischemic bowel disease (IB) typically results in similar changes within the gut, we investigated the relationship between CDI and IB, and the impact of CDI on the clinical outcomes of IB.

Patients And Methods: We initially performed a cross-sectional analysis on the 2014 Healthcare Cost and Utilization Project - Nationwide Inpatient Sample (NIS) patient's discharge records of ages 18 years and older, by estimating the crude and adjusted odds ratio (aOR) of CDI and IB as the outcome and predictor respectively. Read More

View Article
January 2019
1 Read

Clostridium difficile toxins induce VEGF-A and vascular permeability to promote disease pathogenesis.

Nat Microbiol 2018 Dec 3. Epub 2018 Dec 3.

Division of Gastroenterology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.

Clostridium difficile infection (CDI) is mediated by two major exotoxins, toxin A (TcdA) and toxin B (TcdB), that damage the colonic epithelial barrier and induce inflammatory responses. The function of the colonic vascular barrier during CDI has been relatively understudied. Here we report increased colonic vascular permeability in CDI mice and elevated vascular endothelial growth factor A (VEGF-A), which was induced in vivo by infection with TcdA- and/or TcdB-producing C. Read More

View Article
December 2018

The impact of co-infection on colonisation of the chicken.

Infect Immun 2018 Dec 3. Epub 2018 Dec 3.

Pathobiology and Population Sciences, Royal Veterinary College, Hawkshead Lane, North Mymms, AL9 7TA, UK.

can cause the disease coccidiosis in chickens. The direct and often detrimental impact of this parasite on chicken health, welfare and productivity is well recognised, however less is known about the secondary effects infection may have on other gut pathogens. is the leading cause of human bacterial food-borne disease in many countries and has been demonstrated to exert negative effects on poultry welfare and production in some broiler lines. Read More

View Article
December 2018
1 Read

Ferredoxin-linked flavoenzyme defines a family of pyridine nucleotide-independent thioredoxin reductases.

Proc Natl Acad Sci U S A 2018 Dec 3. Epub 2018 Dec 3.

Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas, 37008 Salamanca, Spain

Ferredoxin-dependent thioredoxin reductase was identified 35 y ago in the fermentative bacterium [Hammel KE, Cornwell KL, Buchanan BB (1983) 80:3681-3685]. The enzyme, a flavoprotein, was strictly dependent on ferredoxin as reductant and was inactive with either NADPH or NADH. This early work has not been further pursued. Read More

View Article
December 2018

Environmental disinfection with photocatalyst as an adjunctive measure to control transmission of methicillin-resistant Staphylococcus aureus: a prospective cohort study in a high-incidence setting.

BMC Infect Dis 2018 Dec 3;18(1):610. Epub 2018 Dec 3.

Department of Internal Medicine and AIDS Research Institute, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.

Background: Environmental disinfection with continuously antimicrobial surfaces could offer superior control of surface bioburden. We sought to decide the efficacy of photocatalyst antimicrobial coating in reducing methicillin-resistant Staphylococcus aureus (MRSA) acquisition in high incidence setting.

Methods: We performed prospective cohort study involving patients hospitalized in medical intensive care unit. Read More

View Article
December 2018

Food waste fermentation in a leach bed reactor: Reactor performance, and microbial ecology and dynamics.

Bioresour Technol 2018 Nov 19;274:153-161. Epub 2018 Nov 19.

Department of Civil and Environmental Engineering, University of Waterloo, 200 University Avenue West, Ontario N2L3G1, Canada. Electronic address:

Food waste fermentation was investigated in a leach bed reactor operated at acidic, neutral and alkaline conditions. Highest solids reduction of 87% was obtained at pH 7 in 14 days of reaction time with minimum mixing. The concentration of volatile fatty acids increased to 28. Read More

View Article
November 2018

Impacts of dietary silver nanoparticles and probiotic administration on the microbiota of an in-vitro gut model.

Environ Pollut 2018 Nov 12;245:754-763. Epub 2018 Nov 12.

Department of Food Environmental and Nutritional Sciences, Università degli Studi di Milano, via Celoria 2, 20133, Milano, Italy.

Ingestion of silver nanoparticles (AgNPs) is inevitable linked to their widespread use in food, medicines and other consumer products. However, their effects on human microbiota at non-lethal concentrations remain poorly understood. In this study, the interactions among 1 μg mL AgNPs, the intestinal microbiota, and the probiotic Bacillus subtilis (BS) were tested using in-vitro batch fermentation models inoculated with human fecal matter. Read More

View Article
November 2018
2 Reads

An enhanced green fluorescence protein (EGFP)-based reporter assay for quantitative detection of sporulation in Clostridium perfringens SM101.

Int J Food Microbiol 2018 Nov 19;291:144-150. Epub 2018 Nov 19.

Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Ourai Kita, Izumisano, Osaka 598-8531, Japan. Electronic address:

Clostridium perfringens type F is a spore-forming anaerobe that causes bacterial food-borne illness in humans. The disease develops when ingested vegetative cells reach the intestinal tract and begin to form spores that produce the diarrheagenic C. perfringens enterotoxin (CPE). Read More

View Article
November 2018

A series of three cases of severe Clostridium difficile infection in Australia associated with a binary toxin producing clade 2 ribotype 251 strain.

Anaerobe 2018 Nov 27. Epub 2018 Nov 27.

School of Veterinary & Life Sciences, Murdoch University, Murdoch, WA, 6150, Australia; School of Biomedical Sciences, The University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, WA, Australia; Department of Microbiology, PathWest Laboratory Medicine, Queen Elizabeth II Medical Centre, Nedlands, WA, 6009, Australia; School of Medical & Health Sciences, Edith Cowan University, Joondalup, WA, 6027, Australia.

Three patients with severe Clostridium difficile infection (CDI) caused by an unusual strain of C. difficile, PCR ribotype (RT) 251, were identified in New South Wales, Australia. All cases presented with severe diarrhoea, two had multiple recurrences and one died following a colectomy. Read More

View Article
November 2018

N-acetyltransferases from three different organisms displaying distinct selectivity toward hexosamines and N-terminal amine of peptides.

Carbohydr Res 2018 Nov 22;472:72-75. Epub 2018 Nov 22.

College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin, 300353, PR China. Electronic address:

N-acetyltransferases are a family of enzymes that catalyze the transfer of the acetyl moiety (COCH) from acetyl coenzyme A (Acetyl-CoA) to a primary amine of acceptor substrates from small molecules such as aminoglycoside to macromolecules of various proteins. In this study, the substrate selectivity of three N-acetyltransferases falling into different phylogenetic groups was probed against a series of hexosamines and synthetic peptides. GlmA from Clostridium acetobutylicum and RmNag from Rhizomucor miehei, which have been defined as glucosamine N-acetyltransferases, were herein demonstrated to be also capable of acetylating the free amino group on the very first glycine residue of peptide in spite of varied catalytic efficiency. Read More

View Article
November 2018
1 Read

The Gut Microbiome: A Difficult Target for Translational Studies of Clostridium Difficile Colonization.

J Pediatr Gastroenterol Nutr 2018 Nov 27. Epub 2018 Nov 27.

Inflammatory Bowel Disease Center, Division of Gastroenterology, Hepatology and Nutrition, Boston's Children's Hospital, Boston, MA, USA.

View Article
November 2018

Prevalence of Clostridium perfringens netE and netF toxin genes in the feces of dogs with acute hemorrhagic diarrhea syndrome.

J Vet Intern Med 2018 Nov 30. Epub 2018 Nov 30.

Department of Clinical Veterinary Medicine, Clinic of Small Animal Internal Medicine, Ludwig Maximilian University of Munich, Munich, Germany.

Background: Recently, novel pore-forming toxin genes designated netE and netF were identified in a Clostridium perfringens type A strain isolated from a dog with acute hemorrhagic diarrhea.

Objectives: Pore-forming toxins could play an important role in the disease pattern of acute hemorrhagic diarrhea syndrome (AHDS) in dogs. Thus, we aimed to determine the prevalence of C. Read More

View Article
November 2018

[Change of bacterial community structure during cellulose degradation by the microbial consortium].

Sheng Wu Gong Cheng Xue Bao 2018 Nov;34(11):1794-1808

Heilongjiang Provincial Engineering Technology Research Center of Straws Resources Utilization, Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Agro-Waste in Cold Region, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China.

In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth. We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes. Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively. Read More

View Article
November 2018

Management of adult Clostridium difficile digestive contaminations: a literature review.

Eur J Clin Microbiol Infect Dis 2018 Nov 29. Epub 2018 Nov 29.

Institut de Chimie Radicalaire ICR, UMR CNRS 7273, Laboratoire de Pharmaco-Chimie Radicalaire, Faculté de Pharmacie, Aix Marseille Université, 27 Boulevard Jean Moulin-CS 30064, 13385, Marseille Cedex 05, France.

Clostridium difficile infections (CDI) dramatically increased during the last decade and cause a major public health problem. Current treatments are limited by the high disease recurrence rate, severity of clinical forms, disruption of the gut microbiota, and colonization by vancomycin-resistant enterococci (VRE). In this review, we resumed current treatment options from official recommendation to promising alternatives available in the management of adult CDI, with regard to severity and recurring or non-recurring character of the infection. Read More

View Article
November 2018
4 Reads

High Clostridium difficile contamination rates of domestic and imported potatoes compared to some other vegetables in Slovenia.

Food Microbiol 2019 Apr 30;78:194-200. Epub 2018 Oct 30.

National Laboratory for Health, Environment and Food, Prvomajska 1, 2000 Maribor, Slovenia; Medical Faculty Maribor, University of Maribor, Taborska 8, 2000 Maribor, Slovenia. Electronic address:

Clostridium difficile, recently reclassified to Clostridioides difficile, is among most important causes of intestinal infections in humans. Zoonotic potential and foodborne transmissions are considered to be partially involved in C. difficile spread. Read More

View Article

Use of fluorescent CTP1L endolysin cell wall-binding domain to study the evolution of Clostridium tyrobutyricum during cheese ripening.

Food Microbiol 2019 Apr 29;78:11-17. Epub 2018 Sep 29.

Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Departamento de Tecnología de Alimentos, Carretera de La Coruña Km 7, 28040, Madrid, Spain.

Clostridium tyrobutyricum is a bacteria of concern in the cheese industry, capable of surviving the manufacturing process and causing butyric acid fermentation and late blowing defect of cheese. In this work, we implement a method based on the cell wall-binding domain (CBD) of endolysin CTP1L, which detects C. tyrobutyricum, to monitor its evolution in cheeses challenged with clostridial spores and in the presence or absence of reuterin, an anti-clostridial agent. Read More

View Article

Effect of process parameters on enhanced biohydrogen production from tequila vinasse via the lactate-acetate pathway.

Bioresour Technol 2018 Nov 15;273:618-626. Epub 2018 Nov 15.

Department of Environmental Technology, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Av. Normalistas 800, Col. Colinas de la Normal, C.P. 44270, Guadalajara, Jalisco, Mexico. Electronic address:

In this study, a lactate-type fermentation entailing the consumption of lactate and acetate (lactate-acetate pathway) is proposed to deal with lactic acid bacteria (LAB) inhibition during the production of biohydrogen (bioH) from tequila vinasse. The effects of total solids content, substrate concentration, nutrient formulation and inoculum addition on bioH production performance were investigated. Batch experiments were performed in a 3-L completely mixed reactor at 35 °C and pH 6. Read More

View Article
November 2018

Gut Microbiota Modulates Interactions Between Polychlorinated Biphenyls and Bile Acid Homeostasis.

Toxicol Sci 2018 Dec;166(2):269-287

Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington 98105.

The gut microbiome is increasingly recognized as a second genome that contributes to the health and diseases of the host. A major function of the gut microbiota is to convert primary bile acids (BAs) produced from cholesterol in the liver into secondary BAs that activate distinct host receptors to modulate xenobiotic metabolism and energy homeostasis. The goal of this study was to investigate to what extent oral exposure to an environmentally relevant polychlorinated biphenyl (PCBs mixture), namely the Fox River mixture, impacts gut microbiome and BA homeostasis. Read More

View Article
December 2018

Effects of dietary supplementation of alfalfa meal on growth performance, carcass characteristics, meat and egg quality, and intestinal microbiota in Beijing-you chicken.

Poult Sci 2018 Nov 29. Epub 2018 Nov 29.

Beijing Research and Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.

This study was conducted to investigate the effects of alfalfa meal supplementation on growth performance, carcass characteristics, meat and egg quality, and intestinal microbiota in chickens. A total of 600 healthy 20-wk female Beijing-you chickens (a local Chinese chicken breed) were selected and randomly assigned into 4 dietary treatments: 0, 5, 8, and 10% alfalfa meal supplementation. Chickens were raised in a free-range system for 56 d. Read More

View Article
November 2018
1 Read

The first molecular detection of Clostridium perfringens from pneumonic cases associated with foot and mouth disease in cattle and buffalo in Egypt.

Trop Anim Health Prod 2018 Nov 28. Epub 2018 Nov 28.

Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.

Panting syndrome and respiratory infection have been recorded in complicated cases of foot and mouth disease (FMD) in cattle. However, investigations on the causative agents of respiratory disease in such cases are scarce. In this study, 30 animals (13 buffalo and 17 cattle) suffering from respiratory distress associated with signs of FMD were examined. Read More

View Article
November 2018
1 Read
0.820 Impact Factor

Effects of proton pump inhibitor on the human gut microbiome profile in multi-ethnic groups in Singapore.

Singapore Med J 2018 Nov 29. Epub 2018 Nov 29.

Department of Gastroenterology and Hepatology, Changi General Hospital, Singapore.

Introduction: The objectives of this study were to examine the effects of ethnicity, gender, and a proton pump inhibitor (PPI), omeprazole, on the human gut microbiome. PPIs are commonly used for the treatment of acid-related disorders. We hypothesised that PPI therapy might perturb microbial communities and alter the gut microbiome. Read More

View Article
November 2018
1 Read

Outpatient Parenteral Therapy for Complicated Infections: A Snapshot of Processes and Outcomes in the Real World.

Open Forum Infect Dis 2018 Nov 24;5(11):ofy274. Epub 2018 Oct 24.

Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland.

Background: In the United States, patients discharged on outpatient parenteral antimicrobial therapy (OPAT) are often treated by home health companies (HHCs) or skilled nursing facilities (SNFs). Little is known about differences in processes and outcomes between these sites of care.

Methods: We performed a retrospective study of 107 patients with complicated infections discharged on OPAT from 2 academic medical centers. Read More

View Article
November 2018

Different Influences of Bacterial Communities on Fe (III) Reduction and Phosphorus Availability in Sediments of the Cyanobacteria- and Macrophyte-Dominated Zones.

Front Microbiol 2018 14;9:2636. Epub 2018 Nov 14.

Department of Civil and Environmental Engineering, Faculty of Construction and Environment, The Hong Kong Polytechnic University, Kowloon, China.

Little is known about the effects of bacterial community on iron (Fe) and phosphorus (P) cycles in sediments under different primary producer habitats in different seasons. Lake Taihu has both the cyanobacteria- and macrophyte-dominated lake zones. In this work, the abundance and structure of bacterial community was investigated using qPCR and 16S rRNA gene high throughput sequencing, respectively. Read More

View Article
November 2018