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    2404 results match your criteria Clinical laboratory[Journal]

    1 OF 49

    Verification of Bioanalytical Method for Quantification of Exogenous Insulin (Insulin Aspart) by the Analyser Advia Centaur® XP.
    Clin Lab 2018 Mar;64(3):399-403
    Background: In a number of cases the monitoring of patients with type I diabetes mellitus requires measurement of the exogenous insulin levels. For the purpose of a clinical investigation of the efficacy of a medical device for application of exogenous insulin aspart, a verification of the method for measurement of this synthetic analogue of the hormone was needed. The information in the available medical literature for the measurement of the different exogenous insulin analogs is insufficient. Read More

    Erythropoietin Protects Erythrocytes Against Oxidative Stress-Induced Eryptosis In Vitro.
    Clin Lab 2018 Mar;64(3):365-369
    Background: Excessive eryptosis has been found in maintained hemodialysis or peritoneal dialysis patients. Signaling of triggering eryptosis includes oxidative stress, increased cytosolic Ca2+-activity, and ceramide. Erythropoietin (EPO) possesses the property of an antioxidant. Read More

    Serum CEA and CA19-9 Levels are Associated with the Presence and Severity of Colorectal Neoplasia.
    Clin Lab 2018 Mar;64(3):351-356
    Background: High concentrations of carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9) were found in the serum of patients with colon cancer. We performed the present work to investigate the association between elevated levels of serum biomarkers (CEA and CA19-9) and shortened survival in patients with colon cancer.

    Methods: We examined patients who underwent colonoscopies between 2001 and 2014 and measured and analyzed the serum CEA and CA19-9 levels of 362 participants. Read More

    Performance Verification of Alternative Quality Control Materials for Urine Albumin Assessment.
    Clin Lab 2018 Mar;64(3):345-349
    Background: Accurate detection of urine albumin is important for evaluating the progression of diabetic kidney disease. However, two levels of daily quality control may not be practically feasible in some small clinical laboratories owing to a small number of patient samples and high costs. We aimed to prepare homemade quality control material (HQM) to measure urine albumin and then verify its performance. Read More

    Concordance of Capillary Electrophoresis and Conventional Gel Electrophoresis in Two Different Groups of Patients with.
    Clin Lab 2018 Mar;64(3):339-344
    Background: Serum and urinary protein electrophoresis play an important role in the identification of monoclonal gammopathy. Recently, capillary electrophoresis (CE) has been adapted in many clinical laboratories because of several advantages such as short turnaround time, automation, and high reproducibility. However, there have been unsolved concerns for the concordance between conventional gel and automated capillary electrophoresis methods for protein separation in clinical specimens. Read More

    Retrospective Analysis of Thyroid Nodules: Thyroid Cancer Risk Factors in Suzhou, China.
    Clin Lab 2018 Mar;64(3):333-338
    Background: Thyroid nodules are common, frequently discovered in clinical practice and the incidence has increased in recent decades. They are clinically important primarily due to their malignant potential, because 2 to 5% are malignant. Correct identification of the malignancy in thyroid nodules is a diagnostic challenge, leading to potentially unnecessary surgery in patients for whom final histology is benign. Read More

    Investigation of Iron Metabolism for Regulating Megakaryopoiesis and Platelet Count According to the Mechanisms of Anemia.
    Clin Lab 2018 Mar;64(3):329-332
    Background: Iron deficiency anemia (IDA) is characterized by depletion of total body iron stores or a poor supply of plasma iron. By contrast, chronic inflammation makes iron unavailable for hematopoiesis through a cytokinemediated cascade and leads to a condition known as anemia of chronic disease (AOC). However, the laboratory data regarding the regulatory role of iron metabolism on platelet count has not been fully discussed yet. Read More

    Low Serum Levels of Pre-Surgical Total Cholesterol are Associated with Unfavorable Overall Survival in Patients with Operable Non-Small Cell Lung Cancer.
    Clin Lab 2018 Mar;64(3):321-327
    Background: Cholesterol is an essential building block of the cell membrane and an important molecule for cell signaling and function. The dysregulation of cholesterol metabolism has been linked to several diseases, including cancer. The aim of this study is to investigate whether serum cholesterol is associated with the survival outcomes of patients with non-small cell lung cancer (NSCLC). Read More

    Evaluation of Samples with DFS Staining Pattern Detected by Indirect Immunofluorescence Assay.
    Clin Lab 2018 Mar;64(3):393-397
    Background: In this study, we aimed to determine the presence of anti DFS70 antibody by a specific IB method in samples showing the DFS pattern and to determine the distribution of DFS pattern in different patient groups.

    Methods: 2,401 serum samples, which were received for ANA screening, were tested by IIF method at Akdeniz University Hospital Diagnostic Laboratory. Out of 139 samples with DFS pattern, 75 samples were tested for the presence of anti DFS70 antibody by IB and were included in the study. Read More

    Upregulation of Breast Cancer Resistance Protein Expression was Decreased in Plasma Membrane of Colon Cancer with Metastasis of Lymphatic Node.
    Clin Lab 2018 Mar;64(3):311-319
    Background: ATP-binding cassette sub-family G member 2 (ABCG2), an ABC transport protein involved in the efflux of anticancer compounds, has been reported to have altered expression levels in several cancers including breast, colon, and gastric cancer, etc. However, its expression change (up- or down-regulated in cancer) is still contradictory.

    Methods: We performed immunohistochemistry to examine ABCG2 expression in the microarray with 90 pairs of colon cancer and their adjacent normal tissues. Read More

    Mutations in Core Gene Region of Hepatitis B Virus in Patients with Chronic Hepatitis B.
    Clin Lab 2018 Mar;64(3):303-310
    Background: The HBV core protein plays a major role in host immune response. Mutations occurring in the HBV core gene may cause alterations in the major epitopes being effective in the host immune response. Until now, the persistent effects of core gene mutations on HBV infections have not been fully understood. Read More

    Combination of MicroRNAs and Cytokines: a Method for Better Evaluation of Acute-on-Chronic Liver Failure.
    Clin Lab 2018 Mar;64(3):247-256
    Background: The aim was to establish expression profiles of microRNAs (miRNAs) in Peripheral Blood Mononuclear Cells (PBMC) and of plasma cytokines from the patients with hepatitis B virus related acute-on-chronic liver failure (HBV-ACLF) and high-risk of acute-on-chronic liver failure (ACLF) patients as well as healthy people and to examine the relationships between these profiles and clinical features.

    Methods: Herein, we collected PBMCs and plasma from peripheral blood of HBV-ACLF and ACLF patients as well as healthy people. Microarray, real-time qPCR, and ELISA assays were used. Read More

    Identification of a Novel Nonsense Mutation in a Patient with Transfusion-Dependent Hb H Disease.
    Clin Lab 2018 Mar;64(3):371-374
    Background: Hb H disease is a form of α-thalassemia. The high clinical variability is influenced by the exact combination of mutations. Here we report on a 29-year-old female patient from Afghanistan who received regular blood transfusions since her childhood. Read More

    Comparison of New Liquid Chromatography-Mass Spectrometry Method and Enzyme-Multiplied Immunoassay Technique for Routine Therapeutic Drug Monitoring of Vancomycin in Chinese Patients.
    Clin Lab 2018 03;64(3):277-285
    Background: Therapeutic drug monitoring of vancomycin is very valuable due to the good correlation between trough levels and clinical outcome. Therefore, it is important to accurately determine the concentration of vancomycin in patient plasma for adequate dose-adjustment. The objective of this study was to develop a new liquid chromatography-mass spectrometry (LC-MS) method for determination of vancomycin in patient plasma and compare the results with those obtained from enzyme-multiplied immunoassay technique (EMIT). Read More

    A Proposed Kinetic Model for the Diagnostic and Prognostic Value of WT1 and p53 in Acute Myeloid Leukemia.
    Clin Lab 2018 Mar;64(3):357-363
    Background: Wilms tumor (WT1) and p53 proteins were identified in the pathogenesis of several malignancies, including hematological malignancies. As a result of their interaction and diverse context-specific functions, this study aimed to emphasize the diagnostic and prognostic impacts of WT1 and p53 expression in acute myeloid leukemia (AML).

    Methods: Twelve bone marrow (BM) biopsies were obtained from AML patients who were diagnosed in accordance with the French-American-British diagnostic criteria. Read More

    Using Blood and Plasma MicroRNAs as a Non-Invasive Biomarker in Patients with Colorectal Cancer.
    Clin Lab 2018 Mar;64(3):257-262
    Background: A high percentage of oncological patients die yearly because of colorectal cancer (CRC). Worldwide, CRC represents the fourth leading cause of death among oncological patients. Numerous studies have been conducted in order to identify new biomarkers for the early diagnosis of patients with CRC. Read More

    Low Serum Albumin Correlates with Poor Survival in Gastric Cancer Patients.
    Clin Lab 2018 Mar;64(3):239-245
    Background: Preoperative nutritional status as evidenced by serum albumin measurement is associated with cancer prognosis but the clinical significance of this for patients with gastric cancer (GC) is unclear. Therefore, we evaluated an association between preoperative serum albumin and GC patient survival in the Fujian area which has a higher incidence for GC in China.

    Methods: GC patients (n = 309) who underwent surgical treatment at Fuzhou General Hospital between 2010 and 2013 were retrospectively assessed using a Kaplan-Meier method and log-rank test, as well as univariate and multivariate Cox model analyses, to confirm a correlation between patient survival and preoperative serum albumin. Read More

    Impact of a Missense Variation (p.S150R:AGC>AGG) in the XRCC2 Gene on Susceptibility to Colorectal Cancer.
    Clin Lab 2018 Mar;64(3):233-237
    Background: The X-ray repair complementing defective repair in Chinese hamster cells 2 (XRCC2) is an important protein in response to DNA double-strand breaks (DSBs) in human cells. XRCC2, as a functional protein in the homologous recombination repair (HRR) process, has been identified to have several polymorphisms which might be associated with the risk of cancer. Therefore, we aimed to investigate a novel missense variation (AGC>AGG, p. Read More

    Blood Donation - Are We Willing? - a Cross-Sectional Study Among Dental Students.
    Clin Lab 2018 Mar;64(3):225-232
    Background: To assess the knowledge, attitude, and practice towards blood donation among a cohort of dental students.

    Methods: This cross-sectional study included all the undergraduate and post-graduate dental students of Panineeya Institute of Dental Sciences and Hospital. A self-administered questionnaire comprised of four parts. Read More

    Prevalence of Glucose-6-Phosphate Dehydrogenase Deficiency in Sichuan, China.
    Clin Lab 2018 Mar;64(3):383-386
    Background: Our goals were to screen newborns and characterize the occurrence of glucose-6-phosphate dehydrogenase (G6PD) deficiency in southwestern China. Meanwhile, we would like to analyze the factors that might affect the results of neonatal dried blood spots for glucose-6-phosphate dehydrogenase screening test, to improve the clinical quality control level, effectively reduce the external factors in the process of detection.

    Methods: This study involved an evaluation of G6PD data for 20,644 newborns from a universal newborn screening program. Read More

    Pearson Syndrome, A Medical Diagnosis Difficult to Sustain Without Genetic Testing.
    Clin Lab 2018 Mar;64(3):375-377
    Background: The detection of sideroblastic anemia in a newborn may suggest developing Pearson syndrome. The prognosis of these patients is severe and death occurs in the first 3 years of life, so it is important to find new ways of diagnosis. Case Presentation: In the case of our patient the diagnosis was supported only at the age of 5 months, highlighting the difficulties of diagnosis at this age. Read More

    STAT1 and STAT6 Act as Antagonistic Regulators of PPARγ in Diabetic Patients with and without Cardiovascular Diseases.
    Clin Lab 2018 Mar;64(3):287-294
    Background: The processes that mediate an inflammatory environment and increase atherosclerosis in diabetes are not well understood. Peroxisome proliferator-activated receptors (PPARs) are a subgroup of the nuclear hormone receptor superfamily of ligand-activated transcription factors which play an important role in the pathogenesis of type 2 diabetes mellitus (T2DM) and atherosclerosis. PPARγ promotes changes in lipid metabolism, especially in fatty acid (FA) trafficking, and the activity of PPARγ could be modulated by diabetes phenotype patients. Read More

    Hyperin Enhances the Sensitivity of HCT8/VCR Colon Cancer Cell Line to Vincristine by Down-Regulating P-Glycoprotein.
    Clin Lab 2018 Mar;64(3):269-275
    Background: Long-term chemotherapy reduces the sensitivity of colon cancer cells to chemotherapeutics like vincristine (VCR) and lead to drug resistance, which has become a major barrier for colon cancer treatment. Calcium antagonists are used as clinical tumor multidrug resistance reversal agents to regulate the P-glycoprotein (P-gp) level and block efflux pump function now, but they have significant side effects. Hyperin as active component with low toxicity in traditional Chinese medicine has calcium antagonistic effect. Read More

    Short-Term Effect of High-Dose Pantoprazol on Serum and Urinary Magnesium Levels.
    Clin Lab 2018 Mar;64(3):263-268
    Background: Proton pump inhibitor (PPI) induced hypomagnesemia is a completely unexplained issue and cases are still being reported. Long-term use is the main factor, but there are a few articles stating that it may also emerge with short-term use. We aimed to evaluate the changes of serum and urine magnesium levels during shortterm high dose pantoprazol treatment. Read More

    Comparison of a Newly Developed HPV Genotyping Assay (Mojin HPV Kit) with Cobas 4800 HPV Test for Detection of High-Risk HPV in Specimens Collected in SurePath Solution.
    Clin Lab 2018 Mar;64(3):387-391
    Background: Human papillomavirus (HPV) detection based on cervical cytology specimens is useful for cervical cancer screening. The aim of this study was to compare Mojin HPV kit (a newly developed HPV genotyping assay) with the Cobas 4800 HPV test in detecting high-risk (HR) HPV.

    Methods: A total of 347 cervical exfoliated cell specimens were tested using the Mojin HPV kit and Cobas 4800 HPV test. Read More

    Could Endocan, a Marker of Inflammation and Endothelial Dysfunction, be a New Diagnostic Marker for Fibromyalgia?
    Clin Lab 2018 Mar;64(3):405-410
    Background: Although the pathophysiology of fibromyalgia has not yet been fully clarified, inflammation and endothelial dysfunction have been suggested to occur in patients with fibromyalgia. In recent years, endocan has been reported as an important biomarker for inflammation and endothelial dysfunction. In this study, we investigated this mechanism by measuring the serum endocan levels in fibromyalgia patients. Read More

    Measurement Differences Between Two Immunoassay Systems for LH and FSH: A Comparison of Roche Cobas e601 vs. Abbott Architect i2000sr.
    Clin Lab 2018 Mar;64(3):295-301
    Background: Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) regulate the growth and reproductive activity of gonadal tissue and determine the concentration of LH is essential for the prediction of ovulation. Collectively, FSH and LH are important measurements to ascertain the causes of infertility as well as diagnosing disorders such as polycystic ovary syndrome and pituitary and gonadal dysfunction. This study compares the correlation between LH and FSH measurements during examination with two different systems, Architect i2000sr (Abbott Laboratories; Lake Bluff, IL, USA) and Cobas e601 (Roche; Geneva, Switzerland), and assesses the differences between these systems. Read More

    Distribution of IL28B rs12979860 and rs8099917 Genotypes in Patients with Chronic Hepatitis C Virus Infection in Tianjin, China.
    Clin Lab 2018 Apr;64(4):543-550
    Background: Two single-nucleotide polymorphisms (SNPs) rs12979860 (C/T) and rs8099917 (T/G), near the interleukin 28B (IL28B, also known as IFN lambda-3, IFNL3) gene, have a strong association with both spontaneous and treatment-induced hepatitis C virus (HCV) clearance. IL28B polymorphisms have shown a marked differential distribution with regional and ethnic variation. The study aimed to investigate the distribution of IL28B rs12979860 and rs8099917 genotypes in patients with chronic hepatitis C (CHC) in Tianjin, China. Read More

    Abnormally Expressed Long Non-Coding RNAs in Nasopharyngeal Carcinoma: a Meta-Analysis.
    Clin Lab 2018 Apr;64(4):585-595
    Background: Dysregulated long non-coding RNAs (lncRNAs) have been extensively explored in nasopharyngeal carcinoma (NPC) research. The current study focused on elucidating the overall diagnostic and prognostic performances of abnormally expressed lncRNAs in NPC.

    Methods: We performed a systematic literature search based on the online databases. Read More

    SATB2-Associated Syndrome: a Case Report of a De Novo Nonsense Mutation in SATB2 from China and Review of Literature.
    Clin Lab 2018 Apr;64(4):627-637
    Background: To study the clinical and genetic features from a Chinese child with SATB2-associated syndrome (SAS) and review of literature.

    Methods: The girl, 2 years 3 months old, is admitted to the Department of Pediatric Rehabilitation in our hospital. This patient has mental retardation, language development disorder, cleft palate II0, micrognathia, malocclusion, irritability and bilateral oblique palpebral fissure as a clinical manifestation and is treated for 3 months. Read More

    Different Biological Characteristics of Macrophages from BALB/c Mice and Severe Combined Immunodeficient Mice.
    Clin Lab 2018 Apr;64(4):535-541
    Background: Our previous research has found that absence of lymphocytes during development may affect innate activity of immune cells. To better understand the effect of lymphocyte defects on macrophages during development, we compared the biological characteristics of peritoneal macrophages (PMa) from BALB/c and severe combined immunodeficient (SCID) mice.

    Methods: Cytokines such as TNF-α, IL-6, and IL-10 produced by PMa and mRNA of those cytokines of PMa, phagocytosis of chicken red cells by PMa, and cell surface markers of PMa from BALB/c and SCID mice were tested. Read More

    Expression Analysis of MicroRNA-222 in Breast Cancer.
    Clin Lab 2018 Apr;64(4):491-496
    Background: miR-221 and miR-222 are homologous miRNAs located in tandem, within 1 kb from each other, on human x chromosome. Recent studies declared that microRNA-222 is aberrantly expressed in various malignancies. The goal of this research was to measure the expression level of has-miR-222-3P and reveal its diagnostic and prognostic importance in breast malignancy. Read More

    miR-342-5p Expression Levels in Coronary Artery Disease Patients and its Association with Inflammatory Cytokines.
    Clin Lab 2018 Apr;64(4):603-609
    Background: Atherosclerosis is a progressive inflammatory disease and is the main underlying mechanism of coronary artery disease (CAD). Immune system cells and cytokines play pivotal roles in the development of atherosclerosis. Several studies have shown the role of microRNA in the inflammatory processes of atherosclerosis, and miR-342-5p has been shown to be involved in macrophage activation during atherosclerosis and cytokine secretion. Read More

    Thrombotic Microangiopathy-Like Hemolysis in Vitamin B12 Deficiency-Related Macrocytic Anemia.
    Clin Lab 2018 Apr;64(4):639-643
    Background: Hemolytic features in patients with pernicious anemia have not been emphasized.

    Methods: Seven Japanese patients at 60 - 88 years of age with vitamin B12 deficiency-related hemolytic anemia were assessed.

    Results: Serum vitamin B12 levels in these cases were 46 - 89 pg/mL (normal reference range: 233 - 914 pg/mL). Read More

    Human Neutrophil Alloantigen Genotype Frequencies Among Syrian Population.
    Clin Lab 2018 Apr;64(4):597-601
    Background: Human neutrophil antigens (HNA) are able to provoke allo- and autoimmune antibodies which lead to reactions like autoimmune and neonatal neutropenia. However, until now no data about HNA genotype distribution in Syrian population exists. The aim of this study was to determine the HNA allele frequencies in the largest group asking for asylum in Germany since 2015. Read More

    MiR-300 Serves as Potential Biomarker to Screen Knee Osteoarthritis Patients by Targeting TNFα.
    Clin Lab 2018 Apr;64(4):577-584
    Background: This study mainly explored the serum level of miR-300 with the risk of knee OA, thereby evaluating their diagnostic ability for treatment of knee osteoarthritis (KOA) patients.

    Methods: In the current study, we evaluated the level of TNFα in KOA patients and HCs. The serum was used to quantify the level of TNF-α by way of a sandwich enzyme-linked immunosorbent assay. Read More

    Cerebral Venous Sinus Thrombosis with Cerebral Hemorrhage Presenting with Status Epilepticus in Early Pregnancy.
    Clin Lab 2018 Apr;64(4):611-614
    Background: CVST is an important cause of stroke during late pregnancy and in the puerperium, but it is seldom encountered during early pregnancy. We report a case of a 34-year-old woman presenting with convulsive status epilepticus in early pregnancy.

    Methods: In this case, the cranial computed tomography detected hemorrhage. Read More

    Detection Performance of Circulating MicroRNA-210 for Renal Cell Carcinoma: a Meta-Analysis.
    Clin Lab 2018 Apr;64(4):569-576
    Background: Renal cell carcinoma (RCC) is the most common neoplasm of the adult kidney. miR-210 was a known oncogene with tumor-promoting effects in many types of cancer. This meta-analysis aimed to evaluate the potential diagnostic value of circulating miR-210. Read More

    Molecular Mechanism Exploration of Ischemic Stroke by Integrating mRNA and miRNA Expression Profiles.
    Clin Lab 2018 Apr;64(4):559-568
    Background: Stroke is one of the leading causes of death worldwide, and ischemic stroke accounts for about 80 percent of all cases. Ischemic stroke is usually caused by a blockage in an artery to the brain. However, its molecular mechanisms remain largely unclear. Read More

    Subclasses of Monoclonal (Type I) Immunoglobulin G Cryoglobulins: Report on Two Distinct Cases with Myeloma.
    Clin Lab 2018 Apr;64(4):615-618
    Background: While different clinical manifestations of IgM and IgG monoclonal cryoglobulins have been demonstrated, little is known about the roles of IgG subclasses in the pathophysiology of these conditions.

    Methods: In two cases of myeloma-associated monoclonal (type I) cryoglobulinemia with quite distinct clinical and biological features, serum samples were analyzed using an original IgG subclass-specific immunoblotting technique.

    Results: The first case had painful arthritis of hands and feet, with skin purpura and a sharp decrease of complement C4 level, and the cryoglobulin was of IgG1 subclass. Read More

    Serum Free Light Chain Predict Overall Survival and Response to Therapy in Patients with Newly Diagnosed Multiple Myeloma.
    Clin Lab 2018 Apr;64(4):551-558
    Background: Multiple myeloma is characterized by underlying clinical and biological heterogeneity, which translates to variable responses to treatment and outcome.

    Methods: To assess the roles of serum free light chain (sFLC) and K/L FLC ratio (rFLC) in the diagnoses and prognoses of multiple myeloma (MM), sFLC levels and K/L ratios were measured in 112 patients with newly diagnosed multiple myeloma using the Freelite automated immunoassay.

    Results: Abnormal sFLC and/or rFLC levels were detected 99. Read More

    Hsa-miR-3658 Promotes Cell Proliferation, Migration and Invasion by Effecting LASS2 in Bladder Cancer.
    Clin Lab 2018 Apr;64(4):515-525
    Background: Hsa-miR-3658 is upregulated in various tumors, but its expression in bladder cancer has been rarely studied.

    Methods: In this study, hsa-miR-3658 expressions in several bladder cancer cell lines were examined, and its effect on the malignant degree of bladder cancer and whether hsa-miR-3658 regulates tumor biological behaviors through LASS2 and its downstream molecular pathway were studied and validated.

    Results: It was found miR-3658 expressions differed among different cell lines, which may be an influence factor on the malignancy. Read More

    Review on Vitamin K Deficiency and its Biomarkers: Focus on the Novel Application of PIVKA-II in Clinical Practice.
    Clin Lab 2018 Apr;64(4):413-424
    Background: Vitamin K (VK) is a co-factor of the γ-glutamyl carboxylase that catalyzes the conversion of glutamate residues to γ-carboxyglutamate in VK-dependent proteins. The carboxylation reaction imparts the essential calcium-binding residues for the biological function of several proteins involved in the process of coagulation and bone metabolism. VK deficiency is frequently encountered in newborns and can lead to fatal hemorrhagic complications. Read More

    Circulating Levels of IL-35 and Gene Expression of FoxP3 in Coronary Artery Disease: Is There Any Interplay Between Them and 25-Hydroxyvitamin D3?
    Clin Lab 2018 Apr;64(4):483-490
    Background: We aimed to evaluate interleukin-35 (IL-35) serum levels and the forkhead box P3 (FoxP3) expression in peripheral blood mononuclear cells (PBMCs) of coronary artery disease (CAD) patients compared with the non-CAD group. Also, we examined the possible relationship between gene expression of FoxP3 and serum levels of IL-35 with several CAD-related clinical parameters.

    Methods: This study was conducted on 40 men with CAD and 40 men with a normal coronary artery. Read More

    Quantitative Hepatitis B e Antigen: A Better Predictor of Hepatitis B Virus DNA than Quantitative Hepatitis B Surface Antigen.
    Clin Lab 2018 Apr;64(4):443-449
    Background: Hepatitis B surface antigen is usually secreted by infected hepatocytes in the form of subviral particles rather than infectious virions, while the hepatitis B e antigen originates from the core gene and is modified and secreted by hepatocytes into the circulation and functions as a marker of active viral replication. This study aimed to study the relationship between HBV DNA and quantitative hepatitis B surface and e antigen in Malaysian patients.

    Methods: A total of 82 chronic hepatitis B patients were recruited for this study from the Hepatology Department of Selayang Hospital. Read More

    The Effects of Epstein-Barr Virus Nuclear Antigen 2 (EBNA2) on B-Lymphocytic Activity.
    Clin Lab 2018 Apr;64(4):425-431
    Background: Epstein-Barr virus is a worldwide disease that can cause a wide range of human diseases, the person will become a lifelong carrier of the virus once infected. To investigate the mechanism of Epstein-Barr virus nuclear antigen 2 (EBNA2) on B-lymphocytic activity, sera from 35 patients with infectious mononucleosis and from 34 cases without Epstein-Barr virus infection are collected for experimental analysis.

    Methods: Quantitative real-time PCR, western blot, and MTT assays were used to evaluate the relative expression level of EBNA2 and to examine its impact on cell vitality. Read More

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