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    2230 results match your criteria Clinical laboratory[Journal]

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    Quantification of Renal Function and Cardiovascular Mortality in Patients Admitted to the Emergency Department with Suspected Acute Coronary Syndromes.
    Clin Lab 2017 Sep;63(9):1457-1466
    Background: Increases in the novel serum marker cystatin C are detectable much earlier in the course of chronic kidney disease (CKD) even when levels of serum creatinine are still in the normal range. A major factor causing a decrease in serum creatinine is increasing age. Patients with CKD are more likely to develop cardiovascular disease (CVD) than a healthy population and to suffer premature deaths from CVD related to CKD. Read More

    UGT1A1 Gene Polymorphism Predicts Irinotecan-Induced Severe Neutropenia and Diarrhea in Chinese Cancer Patients.
    Clin Lab 2017 Sep;63(9):1339-1346
    Background: Irinotecan was widely used in colon cancer and lung cancer, etc., and adverse reactions occur some times. The primary aim of this research is to investigate the association between UGT1A1 gene polymorphisms and irinotecan-related adverse effect in Chinese Han population with a novel kind of gene chip technology. Read More

    Novel and Simple Approach to Estimating the Actual Incidence of Blood and Body Fluid Exposure.
    Clin Lab 2017 Sep;63(9):1481-1486
    Background: There is no current way to determine the actual blood and body fluid exposure (BBFE) incidence in hospitals. We propose a simple, reliable, and widely available method for the accurate estimation of BBFE.

    Methods: Data for BBFE for healthcare workers between 2006 and 2015 at Osaka University Hospital were retrospectively extracted from the electronic records. Read More

    Value of Red Cell Distribution Width in Assessing the Severity of Hepatitis B Virus-Related Decompensated Cirrhosis.
    Clin Lab 2017 Sep;63(9):1467-1474
    Background: Hepatitis B virus infection is the main reason for liver cirrhosis in China. Thus, evaluating the disease severity of hepatitis B-related decompensated cirrhosis (HBV-DeCi) is very important. The red cell distribution width (RDW) is thought to be correlated with the severity of various diseases. Read More

    Long Non-Coding RNA Expression Signature Hallmarks Promising Efficacy in Identification of Human Non-Small Cell Lung Cancer: a Meta-Analysis Study.
    Clin Lab 2017 Sep;63(9):1447-1456
    Background: The long non-coding RNAs (lncRNAs) are significantly altered in an expanding list of malignant neoplasms, suggesting that they might be popularized as potential biomarkers for cancer detection. This study sought to validate the diagnostic efficacy of lncRNA expression signature(s) as potential biomarker(s) for non-small cell lung cancer (NSCLC) diagnosis.

    Methods: We conducted the online databases search for all eligible studies. Read More

    Interleukin-24 Concentrations Not Related with Age, but Affect Pro- and Anti-Inflammatory Cytokines Differently in Healthy Donors.
    Clin Lab 2017 Sep;63(9):1379-1391
    Background: Interleukin 24 (IL-24) is expressed at different levels in a variety of tumor tissues and matched normal tissues and is regarded as a potential tumor biomarker as its expression levels in tumor tissues are associated with tumor patient prognosis. At present, the expression level of IL-24 in healthy human peripheral blood is unknown.

    Methods: In this study, 1940 blood samples were collected using different processing methods from healthy donors. Read More

    Interleukin 6 Receptor (IL6-R) Gene Polymorphisms Underlie Susceptibility to Rheumatoid Arthritis.
    Clin Lab 2017 Sep;63(9):1365-1369
    Background: Increased production of interleukin 6 (IL-6) is associated with rheumatoid arthritis that acts through its receptor, IL-6R (interleukin 6 receptor). Various single nucleotide polymorphisms in the IL6R gene conferring susceptibility to rheumatoid arthritis have been identified in various populations yet these associations have not been fully established. The present study was pursued with the aim to evaluate a possible association between three single nucleotide polymorphisms (rs2228145, rs4537545, rs4845617) of the IL6R gene and rheumatoid arthritis in Pakistani patients. Read More

    Increased Expression of Long Noncoding RNA RP11-62F24.2 in Gastric Cancer and Its Clinical Significance.
    Clin Lab 2017 Sep;63(9):1475-1479
    Background: Long noncoding RNAs (lncRNAs) are transcripts longer than 200 nucleotides and have no proteincoding capacity. In recent years, they have been believed to be major players in biological processes. However, there is limited understanding of the many lncRNAs' expressions and their clinical significances in gastric cancer. Read More

    Concomitant Occurrence of Blastic Plasmacytoid Dendritic Cell Neoplasm and Acute Myeloid Leukaemia after Lenalidomide Treatment for.
    Clin Lab 2017 Sep;63(9):1513-1517
    Background: Myelodysplastic syndromes with chromosome 5 long arm deletion (5q-mds) may benefit from lenalidomide treatment. However, unresponsive patients have a high risk for clonal evolution and progression to acute myeloid leukemia. Case: We describe a 5q-patient treated with lenalidomide, who concomitantly developed acute myeloid leukemia and blastic plasmacytoid dendritic cell neoplasm, a rare and highly aggressive lymphoma. Read More

    Bacteremia Caused by Campylobacter Fetus in a Patient With Hepatitis B Virus Infection Complicated with Alcoholic Liver Cirrhosis.
    Clin Lab 2017 Sep;63(9):1523-1525
    Campylobacter fetus (C. fetus) bacteremia is rarely reported. In this article, a 51-year-old Chinese woman with severe hepatitis B virus infection complicated with alcoholic liver cirrhosis was admitted with a 3-month history of fatigue, jaundice, and edema of lower extremity, as well as presenting a high fever. Read More

    Serum IgG4 Concentration in IgG4-Related Disease.
    Clin Lab 2017 Sep;63(9):1323-1337
    Background: IgG4-related disease (IgG4-RD) is an immune-mediated and chronic fibroinflammatory condition that affects almost any organ and often involves multiple organs in the same patient. In this review article, we address the clinical utility of measuring serum immunoglobulin G subclass 4 concentration ([IgG4]) in IgG4-RD diagnosis and in disease monitoring.

    Methods: We discuss the latest literature on the relevance of [IgG4] to the investigation and management of IgG4RDs. Read More

    Impact of Moderate Altitude on Pro-Inflammatory Cytokines in Healthy Volunteers.
    Clin Lab 2017 Sep;63(9):1545-1548
    Background: The induction of microvascular inflammation and the effects on cytokine production in blood due to hypoxia has been shown in the past. We have previously reported a statistically significant increase of the pro-inflammatory cytokine interleukin-8 (IL-8) in normobaric hypoxia in the setting of a hypoxia-chamber. In the present study, we sought to analyze plasma levels of inflammatory cytokines in a real-life stetting in order to foster our knowledge on hypoxia induced microvascular inflammation at moderate altitude. Read More

    Prevalence of ST131 and ST1193 Among Bloodstream Isolates of Escherichia coli not Susceptible to Ciprofloxacin in a Tertiary Care University Hospital in Korea, 2013 - 2014.
    Clin Lab 2017 Sep;63(9):1541-1543
    We investigated the ST distribution of bloodstream isolates of E. coli that were not susceptible to ciprofloxacin (CIP-NS isolates), collected from 2013 to 2014 in a tertiary care hospital. Fifty-nine CIP-NS isolates were collected. Read More

    Systematic Comparison of IgM and IgG ABO Antibody Titers by Using Tube and Gel Card Techniques and its Relevance for ABO-Incompatible Kidney Transplantation.
    Clin Lab 2017 Sep;63(9):1393-1401
    Background: Although the determination of the ABO antibody titers is necessary for the decision-making in ABOincompatible (ABOi) kidney transplantations, various methods for the determination of the ABO antibody titers are being used. However, the absence of uniform standards makes their comparability far more difficult. Two of the most commonly used methods are the tube method and the gel card method. Read More

    Heat Shock Protein 90 Alpha, Lass B Member 1: a Key Regulative Gene of Monitoring Duodenal Tumor for Obesity Induced by High-Fat Diet.
    Clin Lab 2017 Sep;63(9):1357-1364
    Background: Effective methods of preventing and treating duodenal carcinoma, especially cancer induced by obesity (resulting from a high-fat diet) remains a longstanding challenge in medicine.

    Methods: With regard to the objective, key genes were explored in the evolutional process in a group of normal, obese (high-fat diet), and duodenal tumor mice. Here, 23 genes were selected by the bioinformatics method. Read More

    Reference Intervals for Fecal Calprotectin in Adults Using Two Different Extraction Methods in the Uppsala-SCAPIS Cohort.
    Clin Lab 2017 Sep;63(9):1493-1496
    Background: Fecal calprotectin measurement is generally recommended to exclude inflammatory bowel disease (IBD) in patients with suspected IBD. A problem with the fecal calprotectin assays so far has been the rather long test-turnaround times. Recently a particle enhanced turbidimetric immunoassay (PETIA) for fecal calprotectin with assay times of approximately 10 minutes has been introduced on the European market. Read More

    Analysis of Hepatitis C Virus NS5A Region in Patients with Cirrhosis Using an Ultra-Deep Pyrosequencing Method.
    Clin Lab 2017 Sep;63(9):1439-1445
    Background: HCV (Hepatitis C Virus) is genetically more diverse than HBV and HIV (Human Immunodeficiency Virus) and exists as quasispecies within infected individuals. This is due to the lack of efficient proofreading of the viral RNA-dependent RNA polymerase. Consequently, quasispecies emerge depending on the mutation rate of the viral polymerase, which may display a high level of genetic variability in a population. Read More

    National Survey on Internal Quality Control Practice for Lipid Parameters in Laboratories of China from 2014 to 2016.
    Clin Lab 2017 Sep;63(9):1411-1419
    Background: To investigate the situation of Internal Quality Control (IQC) practice for total cholesterol, triglycerides, HDL-cholesterol and LDL-cholesterol from 2014 to 2016 in laboratories in China and provide improvement measurements.

    Methods: A web-based External Quality Assessment (EQA) system was used to collect IQC data of lipid parameters in laboratories which continuously participated in the national EQA programs in China from 2014 to 2016. Pass rate of the coefficients of variation (CVs) of two level quality controls in four lipid parameters were calculated according to six quality specifications for precision to evaluate the current status of precision level of the four lipid parameters and their change over time in China. Read More

    Guillain-Barre Syndrome Induced by Heat Stroke: a Case Report and Literature Review.
    Clin Lab 2017 Sep;63(9):1507-1511
    Background: Heat stroke is a potentially fatal condition that is caused by elevated core temperature. GuillainBarre syndrome (GBS) induced by heat stroke is extremely rare, and its manifestation with damage to multiple cranial nerves has not been described.

    Methods: We describe a rare case of GBS induced by heat stroke with damage to multiple cranial and peripheral nerves. Read More

    A Case of Cutaneous Involvement in T Lymphoblastic Lymphoma Leukemia.
    Clin Lab 2017 Sep;63(9):1497-1500
    Background: Cutaneous involvement is more prevalent in B lymphoblastic lymphoma (B-LBL) than T lymphoblastic lymphoma (T-LBL). The authors describe a rare case of cutaneous involvement in a 25-year-old woman with T lymphoblastic lymphoma leukemia.

    Methods: Hematologic investigation, bone marrow aspirate and biopsy, cytogenetic analysis and cutaneous lesion biopsy were performed. Read More

    Diagnostic Values of sVEGFR-1 and Endostatin in Malignant Pleural Effusions in Patients with Lung Cancer.
    Clin Lab 2017 Sep;63(9):1371-1378
    Background: The diagnosis of malignant pleural effusion (MPE) remains a common clinical challenge because of the sensitivity of conventional cytology for the detection is insufficient. Thus, a sensitive clinical marker for diagnosis is required. The aim of this study was to assess the role of two anti-angiogenic cytokines, soluble vascular endothelial growth factor receptor-1 (sVEGFR-1) and endostatin, in diagnosing MPE. Read More

    miRNAs Expressions and Interaction with Biological Systems in Patients with Alzheimer`s Disease. Using miRNAs as a Diagnosis and Prognosis Biomarker.
    Clin Lab 2017 Sep;63(9):1315-1321
    Background: A high percentage of patients develop Alzheimer`s disease (AD). The main signs are loss of memory and cognitive functions which have a significant impact on lifestyle. Numerous studies have been conducted to identify new biomarkers for early diagnosis of patients with AD. Read More

    Whole Exome Sequencing Identifies a c.C2566T Mutation in the Androgen Receptor in a Chinese Family.
    Clin Lab 2017 Sep;63(9):1533-1539
    Background: Whole exome sequencing (WES) is one of the most valuable tools for the detection of Mendelian diseases in clinical laboratory. We performed WES for a family of 46,XY disorders of gender development and compared the applicability of public databases for the subsequent phenotype studies of WES-identified mutations.

    Methods: DNA samples from the two patients were analyzed by WES. Read More

    Interleukin 20 Gene Polymorphism (rs1518108) is not Associated with Sustained Virological Response in Iranian Patients with Hepatitis C Virus Infection.
    Clin Lab 2017 Sep;63(9):1431-1437
    Methods: This case-control study, was performed on 136 blood samples based on 81 patients with chronic HCV genotypes 1 and 3 including 64 SVR positive and 17 negative and 55 healthy individual controls. DNA was isolated from the samples and the frequency of the polymorphism was analyzed using a PCR-RFLP method. Finally, the products were detected on 3. Read More

    Undetectable Vancomycin Concentrations Utilizing a Particle Enhanced Turbidimetric Inhibition Immunoassay in a Patient with an Elevated IgM Level.
    Clin Lab 2017 Sep;63(9):1527-1532
    Background: A case of undetectable vancomycin concentrations with the use of a particle enhanced turbidimetric inhibition immunoassay is reported.

    Methods: A 73-year-old woman with B-cell lymphoma, chronic neutropenia with myelodysplastic syndrome and elevated IgM levels displayed repeated undetectable vancomycin concentrations, despite appropriate empiric vancomycin dosing. The vancomycin concentrations were processed utilizing a particle enhanced turbidimetric inhibition immunoassay (PETINIA). Read More

    Exogenous Brain-Derived Neurotrophic Factor at a 50 ng/mL Concentration has a Significant Protective Effect on Bilirubin-Induced Cerebral Cortex Neuronal Injury.
    Clin Lab 2017 Sep;63(9):1421-1429
    Background: Bilirubin encephalopathy is a common disabling disease in neonates. Brain-derived neurotrophic factor (BDNF) plays an important role in the repair and regeneration of nerves following injury, as well as the maintenance of neuronal growth and differentiation. This study aimed to investigate the effect of exogenous BDNF on the apoptosis and survival of in vitro cultured neurons injured by bilirubin as well as determine the optimal BDNF concentration. Read More

    Clinical Usefulness of Measuring Red Blood Cell Distribution Width in Patients with Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
    Clin Lab 2017 Sep;63(9):1403-1410
    Background: The red blood cell distribution width (RDW) is increased in chronic liver disease, but its clinical significance in hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is still unclear. The aim of the present study was to investigate the clinical significance of RDW in HBV-ACLF patients.

    Methods: The medical records of HBV-ACLF patients who were admitted to The Second Affiliated Hospital of Anhui Medical University between April 2012 and December 2015 were retrospectively reviewed. Read More

    Functional Genetic Variants in SPHK1 Affect Susceptibility to Gastric Cancer in a Chinese Population.
    Clin Lab 2017 Sep;63(9):1347-1356
    Background: An escalating number of studies have provided identified evidence that sphingosine kinase 1 (SPHK1) plays an essential role in carcinogenesis. This present study was devised to seek the possible correlation of two tag single-nucleotide polymorphisms (SNPs) in SPHK1 (rs3744037 T>C, rs346801 C>T) with the susceptibility to gastric cancer (GC).

    Methods: This present case-control study was comprised of 710 patients with GC and 710 gender- and age-matched cancer-free individuals. Read More

    MDR-1 and CYP3A5 Polymorphisms in Pediatric Idiopathic Nephrotic Syndrome: Impact on Susceptibility and Response to Steroids (Preliminary Results).
    Clin Lab 2017 Jul;63(7):1233-1242
    Background: Oral steroid treatment is the first line of therapy for childhood nephrotic syndrome (NS). However, resistance to this treatment has been observed in some patients. Here, we investigated the association of two steroid metabolism-related genes with susceptibility to childhood NS and the steroid response. Read More

    The Relationship Between Serum TARC, TSLP and POSTN Levels and Childhood Atopic Dermatitis.
    Clin Lab 2017 Jul;63(7):1071-1077
    Background: The aim of the study is to evaluate the relationship between serum periostin (POSTN), thymus and activation-regulated chemokine (TARC), and thymic stromal lymphopoietin (TSLP) levels and disease severity and atopy in children with atopic dermatitis.

    Methods: Sixty children with atopic dermatitis and 31 healthy controls were included in the study. The disease severity was measured by SCORAD scores. Read More

    A 3-Month-Old Child with Acute Gastroenteritis with Enterovirus D68 Detected from Stool Specimen.
    Clin Lab 2017 Jul;63(7):1269-1272
    Enterovirus D68 (EV-D68) is known to be causative agent of mild to severe upper and lower respiratory illnesses in sporadic cases and outbreaks. We present a case report of a 3-month-old child with acute gastroenteritis who visited a pediatric clinic in Kyushu area in Japan in 2015. A stool sample collected from the patient was screened for diarrheal viruses by multiplex RT-PCR. Read More

    Mutational Screening of PKD1 and PKD2 Genes in Iranian Population Diagnosed with Autosomal Dominant Polycystic Kidney Disease.
    Clin Lab 2017 Jul;63(7):1261-1267
    Background: Autosomal Dominant Polycystic Kidney Disease (ADPKD) is the fourth most frequent cause of endstage renal disease (ESDR), occurring at a varying frequency of 1/400 to 1/800 persons. The disease affects all ethnic groups worldwide, and there is a need for population based studies to be carried out for better diagnostic, genetic counselling, and treatment purpose.

    Methods: Eighteen unrelated probands (10 males and 8 females) with a familial history of ADPKD were selected for the study. Read More

    Preparation of Commutable Frozen Human Serum Candidate Reference Materials for Standardization of Sodium Measurements.
    Clin Lab 2017 Jul;63(7):1243-1250
    Background: The measurement of serum sodium is important in the diagnosis of diseases. However, the accuracy and comparability of results in clinical laboratories are not ideal. The aim of this study was to prepare the candidate reference materials (RMs) to standardize serum sodium measurements. Read More

    Investigation of Fc Receptor-Like 3 (FCRL-3) Gene Polymorphism (rs7528684) with Susceptibility to Allergic Asthma in Iranian North-Western Azeri Population.
    Clin Lab 2017 Jul;63(7):1301-1305
    Background: Asthma is a clinical setting in which multiple cellular and molecular mechanisms are involved. Additionally, increasing genetic studies have provided evidence that single nucleotide polymorphisms (SNPs) in asthma relevant genes confer susceptibility to the disease. Fc receptor-like (FCRL) 3, a transmembrane molecule basically involved in B-cell signaling, mediates immune-disorders including allergy. Read More

    External Quality Assessment of First-Trimester Prenatal Biochemical Screening in China.
    Clin Lab 2017 Jul;63(7):1171-1177
    Background: Free β subunit of human chorionic gonadotropin (free β-hCG) and pregnancy-associated plasma protein A (PAPP-A) are two important biomarkers in first-trimester prenatal screening. This study intended to reflect their analytical performance in clinical laboratories and main platforms of the 2015 External Quality Assessment (EQA) scheme for the first-trimester biochemical screening in China.

    Methods: Ten lyophilized EQA samples, divided into two sets and analyzed in two cycles 20151 and 20152, were distributed to each participant in 2015. Read More

    Differential Expression of MicroRNAs in Calcific Aortic Stenosis.
    Clin Lab 2017 Jul;63(7):1163-1170
    Background: Calcific aortic stenosis (CAS) is the most common heart valve disorder. To explore the underlying mechanisms, we investigated whether key microRNAs in calcified aortic valves are differentially expressed compared to those in the non-calcified valves.

    Methods: Calcified aortic valves from patients with aortic stenosis and non-calcified aortic valves (control) from patients with aortic insufficiency (n = 8 per group) were obtained during cardiac valve replacement surgery. Read More

    Effects of Passive Smoking on Glycemic Parameters and Lipid Profiles in a Chinese Female Population.
    Clin Lab 2017 Jul;63(7):1147-1152
    Background: The adverse effects of exposure to second-hand cigarette smoke on glucose and lipid parameters in women and the underlying mechanisms are not well understood. In this prospective community-based study, we examined the effects of passive smoking on glycemic parameters and lipid profiles in a Chinese female population.

    Methods: Of 3197 healthy women enrolled (30 - 75 years), 2082 self-reported passive exposure to smoke (≥ 15 minutes/day, 3 days/week, > 1 year) and 1115 had no smoke exposure (control group). Read More

    Assessment of Glycated Albumin as a Useful Indicator for Renal Dysfunction in Diabetic and Nondiabetic Population.
    Clin Lab 2017 Jul;63(7):1129-1137
    Background: Glycated albumin (GA) reflects serum glucose of the preceding 2 - 3 weeks and plays an important role in diabetes mellitus (DM). This study aimed at investigating whether GA can assess renal dysfunction in population.

    Methods: 3818 individuals attending physical examination were enrolled in this cross-sectional study and divided into five groups: healthy controls, impaired fasting glucose, DM without renal complications, DM with albuminuria, and nondiabetic chronic kidney disease patients. Read More

    Correlation of Fasting Versus Postprandial Plasma Glucose with HbA1c in Chinese Type 2 Diabetic Patients Taking Different Hypoglycemic Agents.
    Clin Lab 2017 Jul;63(7):1293-1300
    Background: Currently, it is not well understood how the contribution of basal versus postprandial plasma glucose changes in the management of type 2 diabetes mellitus (DM) with different treatment regimens. This study aimed to investigate the relationships between glycated hemoglobin (HbA1c) and fasting as well as postprandial blood glucose and to determine how these relationships changed during DM progression in Chinese diabetic patients with different treatment regimens.

    Methods: Continuous glucose monitoring was conducted in 228 type 2 DM patients with stable glucose levels. Read More

    Limitations of Preoperative PFA-200 as a Predictor of Postoperative Blood Loss in Total Knee Arthroplasty: According to Use of Tranexamic Acid.
    Clin Lab 2017 Jul;63(7):1121-1128
    Background: Recently, anti-fibrinolytic agents (tranexamic acid) have been used to reduce blood loss or and the need for transfusion. We evaluated the clinical utility of PFA-200 as a predictor of postoperative blood loss in a clinical setting, where hemostasis can be affected by variables such as existing comorbidities and medication use.

    Methods: We analyzed retrospectively 243 TKA patients having PFA-200 results between March 2014 and May 2016. Read More

    Lack of Association between SLC6A11 Genetic Polymorphisms and Drug Resistant Epilepsy in Chinese Han Population.
    Clin Lab 2017 Jul;63(7):1113-1120
    Background: Epilepsy is one of the most common chronic neurological diseases. A recent study has implicated that the genetic variants of the SLC6A11 gene encoding GAT-3, an astrocytic GABA transporter, may influence the efficacy of antiepileptic drugs (AEDs) in the Korean population.

    Methods: This study aims to investigate the possible associations between SLC6A11 gene and drug resistance in Chinese epilepsy patients. Read More

    Homocysteine Metabolism and Hematological Parameters in Early Stage of Phenytoin Treated Epileptic Children.
    Clin Lab 2017 Jul;63(7):1089-1097
    Background: Long-term antiepileptic drug (AED) therapy has been associated with metabolic consequences that lead to an increase in risk of atherosclerosis in patients with epilepsy. Earlier published studies showed conflicting results about the levels of hematological parameters, serum homocysteine, folate, and vitamin B12, in epileptics treated with phenytoin monotherapy. Therefore, we evaluated homocysteine metabolism and hematological parameters in early stage of phenytoin treated epileptic children. Read More

    Epigenetic Modification of MicroRNA-205 and its Association with Glioblastoma Multiform.
    Clin Lab 2017 Jul;63(7):1079-1088
    Background: Deregulated expressions of tumor-suppressive microRNAs (miRNAs) by epigenetic aberrations has a critical role in tumorigenesis. The aim of the present study was to investigate the epigenetic aberrations of miR205 and to understand how this modification may contribute to molecular events in glioblastoma multiform (GBM).

    Methods: Quantitative RT-PCR and bisulfite genomic sequencing techniques were used to investigate gene expression and methylation levels of miR-205 in GBM tissues (n = 23), their matched adjacent normal tissues (n = 23) and glioblastoma U87MG cell line. Read More

    Study of MYOC Gene Mutation in POAG Patients in Zahedan, Iran.
    Clin Lab 2017 Jul;63(7):1283-1291
    Background: The second cause of blindness in the world is glaucoma that begins with visual impairments and, in many cases, ends with irreversible visual loss. Primary open-angle glaucoma (POAG) is the most common type of glaucoma, which causes irreversible optic nerve damage in adults. Glaucoma shows an unknown etiology, but there is strong evidence regarding the role of genetic factors in disease establishment. Read More

    A Modified Combination of Two Methods for Isolation of Polymorphonuclear Granulocytes from Human Blood for Polymerase Chain Reaction (PCR).
    Clin Lab 2017 Jul;63(7):1279-1282
    Background: The aim of the study was to determine a rapid and simple method for isolation of polymorphonuclear (PMN) granulocytes from human blood appropriate for PCR of extracted DNA.

    Methods: A modification of the combined dextran plus Ficoll-Hypaque methods was the final method used in 41 samples of normal white cells from humans. The changes encompassed a lower dextran-blood ratio (2:1), a shorter centrifugation time of leukocytes, a small volume of Ficoll-Hypaque suspension, and a decreased speed in the last centrifugation. Read More

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