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    6458 results match your criteria Clinical and Experimental Pharmacology and Physiology[Journal]

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    Morphine hyposensitivity in STZ-diabetic rats: Reversal by dietary L-arginine treatment.
    Clin Exp Pharmacol Physiol 2017 Sep 19. Epub 2017 Sep 19.
    School of Pharmacy, Faculty of Health and Behavioural Sciences, The University of Queensland, St Lucia Campus, Brisbane, Queensland, Australia, 4072.
    Painful diabetic neuropathy (PDN) is a long-term complication of diabetes. Defining symptoms include mechanical allodynia (pain due to light pressure or touch) and morphine hyposensitivity. In our previous work using the streptozotocin (STZ)-diabetic rat model of PDN, morphine hyposensitivity developed in a temporal manner with efficacy abolished at 3-months post-STZ and maintained for 6-months post-STZ. Read More

    MiR-30a-5p ameliorates spinal cord injury-induced inflammatory responses and oxidative stress by targeting Neurod 1 through MAPK/ERK signaling.
    Clin Exp Pharmacol Physiol 2017 Sep 19. Epub 2017 Sep 19.
    Department of Orthopedics, South Campus, Renji Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai, 200001, PR China.
    Spinal cord injury (SCI) is a major disability requiring more effective treatment than is currently available. MicroRNAs have been shown to effectively regulate gene expression at the translational level. The aim of the present study was to explore the potential role of miR-30-5p and possible mechanism in SCI. Read More

    (-)-Epigallocatechin-3-gallate and EZH2 inhibitor GSK343 have similar inhibitory effects and mechanisms of action on colorectal cancer cells.
    Clin Exp Pharmacol Physiol 2017 Sep 19. Epub 2017 Sep 19.
    Laboratory of Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, P. R. China.
    Epigallocatechin-3-gallate (EGCG) is a type of catechin. It exhibits excellent antioxidant effects and anti-tumor activities for cancer chemoprevention. The mechanism of anti-tumor effects of EGCG on different cancers has been studied for the past few decades, but remains controversial. Read More

    Relationship between Urinary Sodium-to-Potassium Ratio and Ambulatory Blood Pressure in Patients with Diabetes Mellitus.
    Clin Exp Pharmacol Physiol 2017 Sep 16. Epub 2017 Sep 16.
    Department of Medicine, The University of Melbourne, Melbourne, VIC, Australia.
    Previous studies investigating the relationship between sodium intake and blood pressure have mostly relied on dietary recall and clinic blood pressure measurement. In this cross-sectional study, we aimed to investigate the relationship between 24h urinary sodium and potassium excretion, and their ratio, with 24h ambulatory blood pressure parameters including nocturnal blood pressure dipping in patients with type 1 and 2 diabetes. We report that in 116 patients with diabetes, systolic blood pressure was significantly predicted by the time of day, age, the interaction between dipping status with time, and 24h urinary sodium-to-potassium ratio (R(2) =0. Read More

    Association of serum osteocalcin levels with major adverse cardiovascular events: a 4.4-year retrospective cohort study.
    Clin Exp Pharmacol Physiol 2017 Sep 16. Epub 2017 Sep 16.
    Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital; Shanghai Clinical Center for Diabetes; Shanghai Key Clinical Center for Metabolic Disease; Shanghai Key Laboratory of Diabetes Mellitus; Shanghai Diabetes Institute, Shanghai, China.
    We investigated whether the serum osteocalcin levels at baseline were associated with the incidence of major adverse cardiovascular events (MACE) in a population-based retrospective cohort study of Chinese subjects. Coronary angiography was used to diagnose coronary artery disease (CAD). Survival curves were analyzed by performing log-rank tests with Kaplan-Meier figures. Read More

    C1q/TNF-related protein 9 improves the anti-contractile effects of perivascular adipose tissue via the AMPK-eNOS pathway in diet-induced obese mice.
    Clin Exp Pharmacol Physiol 2017 Sep 13. Epub 2017 Sep 13.
    Department of Endocrinology, Affiliated Hospital of Weifang Medical University, Weifang, China.
    The anti-contractile property of perivascular adipose tissue (PVAT) is abolished through an endothelium-dependent pathway in obesity. C1q/tumor necrosis factor-related protein (CTRP)9 improved endothelial function by promoting endothelium-dependent vasodilatation. The aims of this study were to investigate whether CTRP9 improves the anti-contractile effect of PVAT and protects against PVAT dysfunction in obese mice. Read More

    The basis of differences in thermodynamic efficiency among skeletal muscles.
    Clin Exp Pharmacol Physiol 2017 Sep 11. Epub 2017 Sep 11.
    Auckland Bioengineering Institute, University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand.
    Muscles convert chemical free energy into mechanical work. The energy conversion occurs in two steps. First, free energy obtained from oxidation of metabolic substrates (ΔGS ) is transferred to ATP and, second, free energy from ATP hydrolysis (ΔGATP ) is converted into work by myosin cross-bridges. Read More

    Curcumin ameliorates severe influenza pneumonia via attenuating lung injury and regulating macrophage cytokines production.
    Clin Exp Pharmacol Physiol 2017 Aug 29. Epub 2017 Aug 29.
    Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu Province, China.
    Curcumin, an active phenolic agent extract from the Curcuma longa, exhibits excellent anti-cancer, anti-inflammation, and neuroprotective effects. We aimed to investigate the anti-influenza role of Curcumin in vitro and in vivo. The effect of Curcumin on replication of influenza A virus (IAV) was examined in human lung cancer cell line A549, as well as in a mouse model. Read More

    Blockade of 5-HT3 receptors with granisetron does not affect trigeminothalamic nociceptive transmission in rats: implication for migraine.
    Clin Exp Pharmacol Physiol 2017 Aug 29. Epub 2017 Aug 29.
    Department of Neuropharmacology, Valdman Institute of Pharmacology, First St. Petersburg Pavlov State Medical University, 6/8 Lev Tolstoy Street, St, Petersburg, 197022, Russia.
    A perspective way to expand the existing range of anti-migraine drugs seems to be the search for pharmacological agents with anti-cephalalgic properties among medicines approved for clinical indications other than migraine. Numerous experimental and clinical data imply that selective serotonin 5-HT3 receptor antagonists can be considered as potential anti-migraine agents. Therefore, the objective of our work was to examine the impact of selective 5-HT3 receptor blockade with granisetron on migraine-related nociceptive transmission within the spinal trigeminal nucleus (STN) and the ventroposteromedial nucleus of the thalamus (VPM). Read More

    (-)-Epigallocatechin-3-gallate and atorvastatin treatment down-regulates liver fibrosis related genes in non-alcoholic fatty liver disease.
    Clin Exp Pharmacol Physiol 2017 Aug 16. Epub 2017 Aug 16.
    Department of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, P. R. China.
    Non-alcoholic fatty liver disease (NAFLD) and associated advanced liver diseases have become prevalent conditions in many countries and are associated with increased mortality. Gene expression profiles in NAFLD have been examined recently but changes in expression elicited by chemical compound treatments have not been investigated. Since (-)-Epigallocatechin-3-gallate (EGCG) and atorvastatin (ATST) exhibit similar efficacy in NAFLD models, we reasoned that some common key genes might alter after treatment of EGCG and ATST. Read More

    Pharmacological modulation of C-X-C motif chemokine receptor 4 influences development of acute respiratory distress syndrome after lung ischemia-reperfusion injury.
    Clin Exp Pharmacol Physiol 2017 Aug 16. Epub 2017 Aug 16.
    Burn and Shock Trauma Research Institute, Department of Surgery, Loyola University Chicago, Stritch School of Medicine.
    Activation of CXC motif chemokine receptor 4 (CXCR4) has been reported to result in lung protective effects in various experimental models. The effects of pharmacological CXCR4 modulation on the development of acute respiratory distress syndrome (ARDS) after lung injury, however, are unknown. Thus, we studied whether blockade and activation of CXCR4 influences development of ARDS in a unilateral lung ischemia-reperfusion injury rat model. Read More

    Celecoxib inhibits osteoblast differentiation independent of cyclooxygenase activity.
    Clin Exp Pharmacol Physiol 2017 Aug 16. Epub 2017 Aug 16.
    Division of Applied Pharmacology, Department of Health Promotion, Kyushu Dental University, Kitakyushu, 803-8580, Japan.
    Non-steroidal anti-inflammatory drugs (NSAIDs) exert their effects primarily by inhibiting the activity of cyclooxygenase (COX), thus suppressing prostaglandin synthesis. Some NSAIDs are known to perform functions other than pain control, such as suppressing tumour cell growth, independent of their COX-inhibiting activity. To identify NSAIDs with COX-independent activity, we examined various NSAIDs for their ability to inhibit osteoblastic differentiation using the mouse pre-osteoblast cell line MC3T3-E1. Read More

    Eulogy John Ludbrook: surgeon, physiologist and biostatistician.
    Clin Exp Pharmacol Physiol 2017 Aug 12. Epub 2017 Aug 12.
    Baker Heart and Diabetes Institute, Melbourne, Australia.
    On the morning of Friday June 9, Professor John Ludbrook died peacefully in his sleep at the age of 87. John will be deeply missed by his family, friends and colleagues. John Ludbrook had a long and distinguished career as a teacher, educator, surgeon, physiologist and lastly as a statistician. Read More

    Micro-computed tomographic analysis of the radial geometry of intrarenal artery-vein pairs in rats and rabbits: Comparison with light microscopy.
    Clin Exp Pharmacol Physiol 2017 Aug 10. Epub 2017 Aug 10.
    Cardiovascular Disease Program, Biomedicine Discovery Institute and Department of Physiology, Monash University, Melbourne, Vic., Australia.
    We assessed the utility of synchrotron-radiation micro-computed tomography (micro-CT) for quantification of the radial geometry of the renal cortical vasculature. The kidneys of nine rats and six rabbits were perfusion fixed and the renal circulation filled with Microfil. In order to assess shrinkage of Microfil, rat kidneys were imaged at the Australian Synchrotron immediately upon tissue preparation and then post fixed in paraformaldehyde and reimaged 24 hours later. Read More

    5-HT modulates the rat mesenteric vasopressor outflow by 5-HT1D sympatholytic receptors.
    Clin Exp Pharmacol Physiol 2017 Aug 3. Epub 2017 Aug 3.
    Laboratory of Pharmacology, Department of Physiology and Pharmacology, Faculty of Pharmacy, University of Salamanca, Salamanca, Spain.
    5-hydroxytryptamine (5-HT) modulates noradrenergic activity in different cardiovascular territories, but its effect on the mesenteric vasopressor outflow has not yet been clarified. This study investigated the in vivo serotonergic influence, characterizing 5-HT receptors implicated, in sympathetic innervation of mesenteric vasculature. Wistar rats were anaesthetised and prepared for the in situ autoperfused rat mesentery, monitoring systemic blood pressure (SBP), heart rate (HR) and mesenteric perfusion pressure (MPP). Read More

    ELR(+) chemokine-mediated neutrophil recruitment is involved in 2,4,6-trinitrochlorobenzene-induced contact hypersensitivity.
    Clin Exp Pharmacol Physiol 2017 Aug 1. Epub 2017 Aug 1.
    Department of Physiology and Molecular Sciences, School of Pharmacy, Hoshi University, Shinagawa-ku, Tokyo, Japan.
    Contact dermatitis is a form of delayed-type hypersensitivity characterized by localized thickening, papules, redness and vesicles of the skin. A model of contact dermatitis involving repeated challenge of a hapten is adapted to assess dermatitis as characterized by skin thickening. Recently, it was reported that neutrophils have crucial roles in contact hypersensitivity. Read More

    Differential expression of two genes Oct-4 and MUC5AC associates with poor outcome in patients with gastric cancer.
    Clin Exp Pharmacol Physiol 2017 Aug 1. Epub 2017 Aug 1.
    General practitioner (GP), Doctor of Medicine (MD), Tehran and Tabriz, Iran.
    Gastric cancer (GC) is the most frequent leading cause of cancer-associated mortality worldwide that is linked to poor prognosis due to the lack of appropriate biomarkers. Our aim was to evaluate the MUC5AC and Oct-4 expression levels in GC and to assess their association with clinical factors. Immunohistochemical analysis (IHC) and qRT-PCR were performed in GC patients to examine the MUC5AC and Oct-4 expression levels. Read More

    The Changes in and Relationship between Plasma Nitric Oxide and Corticotropin-Releasing Hormone in Patients with Major Depressive Disorder.
    Clin Exp Pharmacol Physiol 2017 Jul 29. Epub 2017 Jul 29.
    Department of Psychiatry, The Fourth Affiliated Hospital, Zhejiang University, School of Medicine, Yiwu, 322000, Zhejiang, PR China.
    Because there is strong evidence of roles of the hypothalamus-pituitary-adrenal axis and nitric oxide (NO) synthase-NO system in depression, but the relationship between them is unknown, the aim of this study was to elucidate whether there is any correlation between NO and corticotropin-releasing hormone (CRH) in major depressive disorder (MDD) patients. In 16 outpatients with MDD and 18 healthy controls, the plasma amino acids citrulline (Cit) and arginine (Arg) were determined by high-performance liquid chromatography, and CRH levels was measured by radioimmunoassay. The Cit/Arg ratio was calculated as an index of NO synthesis. Read More

    Spectrum evaluation-assisted eicosanoid metabolomics for global eicosanoid profiling in human vascular endothelial cells.
    Clin Exp Pharmacol Physiol 2017 Jul 29. Epub 2017 Jul 29.
    Tianjin Key Laboratory of Metabolic Diseases, Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin, China.
    Eicosanoids are hundreds of metabolites derived from poly-unsaturated fatty acids (PUFAs), which regulate biological processes from multiple angles via a complex metabolic network. Targeted eicosanoid metabolomics is used to study the eicosanoid profile in biological samples but only for eicosanoids with available standards. To expand the coverage of eicosanoids detected, we identified the eicosanoids without available standards by estimation of the retention time and comparison of the MS/MS spectra with the reference ones which was collected in a database from literature. Read More

    The effect of tocopheryl phosphates (TPM) on the development of atherosclerosis in apolipoprotein-E deficient mice.
    Clin Exp Pharmacol Physiol 2017 Jul 26. Epub 2017 Jul 26.
    Department of Pharmacology, Monash University, Clayton, Melbourne, Victoria, Australia.
    α-Tocopheryl phosphate (TP) is a naturally occurring form of vitamin E found in the body. In the present study we compared the ability of an α-TP mixture (TPM) against a standard vitamin E supplement, α-tocopherol acetate (TA) on the development of atherosclerotic lesions in ApoE-deficient mice. Mice were maintained on either a normal chow diet for 24 weeks (Normal Diet), vs a group in which the final 8 weeks of the 24-week period mice were placed on a high fat (21%), high cholesterol (0. Read More

    Pharmacokinetic evaluation of oral itraconazole for antifungal prophylaxis in children.
    Clin Exp Pharmacol Physiol 2017 Jul 26. Epub 2017 Jul 26.
    Laboratory of Clinical Pharmacology and Pharmacogenetics, Department of Medical Sciences, Unit of Infectious Diseases, University of Torino, ASL Città di Torino, Amedeo di Savoia Hospital, Turin, Italy.
    Itraconazole is a first-generation triazole agent with an extended spectrum of activity; it is licensed in adults for superficial and systemic fungal infections; no recommendation has been yet established for use in children patients. Its variable and unpredictable oral bioavailability make it difficult to determine the optimal dosing regimen. Hence, therapeutic drug monitoring, highly available in clinical practice, may improve itraconazole treatment success and safety. Read More

    Effects of combination of mitiglinide with various oral antidiabetic drugs in streptozotocin-nicotinamide-induced type 2 diabetic rats and Zucker fatty rats.
    Clin Exp Pharmacol Physiol 2017 Jul 26. Epub 2017 Jul 26.
    Pharmacology Research Laboratory, R&D, Kissei Pharmaceutical Co., Ltd., Azumino, Nagano, Japan.
    We examined the effects of combining the rapid insulin secretagogue, mitiglinide, with various oral hypoglycaemic drugs including biguanides, pioglitazone, α-glucosidase inhibitors, and sodium-glucose co-transporter 2 inhibitors in a rat model of type 2 diabetes. The oral glucose tolerance test (OGTT) using glucose, sucrose, or a liquid meal was used to compare the effects of mitiglinide with those of the four oral hypoglycaemic drugs and examine their combined effects on blood glucose levels and insulin secretion in the rat model. The combination of mitiglinide with other oral hypoglycaemic drugs suppressed the plasma glucose levels more than either agent did alone. Read More

    Furosemide suppresses ileal and colonic contractility via interactions with GABA-A receptor in mice.
    Clin Exp Pharmacol Physiol 2017 Jul 26. Epub 2017 Jul 26.
    Department of Physiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
    The loop diuretic furosemide has an action to inhibit Na(+) -K(+) -2Cl(-) co-transporter at the thick ascending limb of Henle's loop resulting in diuresis. Furosemide also has the non-diuretic effects by binding to GABA-A receptor which may involve the gastrointestinal tract. The aim of this study was to investigate the effects of furosemide on smooth muscle contractions in mice ileum and proximal colon. Read More

    NS1643 enhances ionic currents in a G604S-WT hERG co-expression system associated with long QT syndrome 2.
    Clin Exp Pharmacol Physiol 2017 Jul 25. Epub 2017 Jul 25.
    Department of Cardiovascular Medicine, First Affiliated Hospital of Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education, Xi'an, Shaanxi, China.
    Loss of function mutations in the human ether-a-go-go-related gene (hERG) cause long QT syndrome type 2 (LQT2). Most LQT2 patients are heterozygous mutation carriers in which the mutant hERG exerts potent dominant-negative effects. 1, 3-bis-(2-hydroxy-5-trifluoromethyl-phenyl)-urea (NS1643) is known to enhance IKr in WT-hERG. Read More

    Growth inhibitory effect of β-eudesmol on cholangiocarcinoma cells and its potential suppressive effect on heme oxygenase-1 production, STAT1/3 activation, and NF-κB downregulation.
    Clin Exp Pharmacol Physiol 2017 Jul 21. Epub 2017 Jul 21.
    Chulabhorn International College of Medicine, Thammasat University, Pathumthani, Thailand.
    Cholangiocarcinoma (CCA) is a progressively fatal form of cancer originating from the malignant transformation of hepatic biliary cholangiocytes. The present study reports for the first time in vitro growth inhibitory activities of β-eudesmol, the bioactive sesquiterpenoid present in the rhizome of Atractylodes lancea (Thunb) DC., with respect to its underlying potential effects on heme oxygenase-1 (HO-1) production, STAT1/3 phosphorylation, and NF-κB protein expression in human CCA cell line CL-6. Read More

    Specific α7 nicotinic acetylcholine receptor agonist ameliorates isoproterenol-induced cardiac remodelling in mice through TGF-β1/Smad3 pathway.
    Clin Exp Pharmacol Physiol 2017 Jul 21. Epub 2017 Jul 21.
    Department of Pharmacology, Xi'an Jiaotong University, Health Science Centre, Xi'an, China.
    It is well-accepted that inflammation plays an important role in the development of cardiac remodelling and that therapeutic approaches targeting inflammation can inhibit cardiac remodelling. Although a large amount of evidence indicates that activation of α7 nicotinic acetylcholine receptor (α7nAChR) causes an anti-inflammatory effect, the role of α7nAChR in cardiac remodelling and the underlying mechanism have not been established. To investigate the effect of the specific α7nAChR agonist, PNU282987, on cardiac remodelling induced by isoproterenol (ISO 60 mg/kg per day) in mice, the cardiomyocyte cross-sectional area (CSA) and collagen volume fraction were evaluated by hematoxylin and eosin (HE) and Masson staining, respectively. Read More

    Effects of nitric oxide on large-conductance Ca(2+) -activated K(+) currents in human cardiac fibroblasts through PKA and PKG-related pathways.
    Clin Exp Pharmacol Physiol 2017 Jul 21. Epub 2017 Jul 21.
    Department of Physiology, College of Medicine, Chung-Ang University, Seoul, Korea.
    The human cardiac fibroblast (HCF) is the most abundant cell type in the myocardium, and HCFs play critical roles in maintaining normal cardiac function. However, unlike cardiomyocytes, the electrophysiology of HCFs is not well established. In the cardiovascular system, Ca(2+) -activated K(+) (KCa) channels have distinct physiological and pathological functions, and nitric oxide (NO) plays a key role. Read More

    Angiotensin II-preconditioning is associated with increased PKCε/PKCδ ratio and prosurvival kinases in mitochondria.
    Clin Exp Pharmacol Physiol 2017 Jul 14. Epub 2017 Jul 14.
    Department of Physiology, University of Puerto Rico School of Medicine, San Juan, 00936-5067, Puerto Rico.
    Angiotensin II preconditioning (APC) has been shown to reproduce the cardioprotective effects of ischaemic preconditioning (IPC), however, molecular mechanisms mediating the effects of APC remain unknown. In this study, Langendorff-perfused rat hearts were subjected to IPC, APC or both (IPC/APC) followed by ischaemia-reperfusion (IR), to determine translocation of PKCε, PKCδ, Akt, Erk1/2, JNK, p38 MAPK and GSK-3β to mitochondria as an indicator of activation of the protein kinases. In agreement with previous observations, IPC, APC and IPC/APC increased the recovery of left ventricular developed pressure (LVDP), reduced infarct size (IS) and lactate dehydrogenase (LDH) release, compared to controls. Read More

    Dopamine D1 receptors induce apoptosis of osteosarcoma cells via changes of MAPK pathway.
    Clin Exp Pharmacol Physiol 2017 Jul 12. Epub 2017 Jul 12.
    Department of Orthopaedic Surgery of Emergency, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, China.
    This study explored the effects and mechanisms of dopamine D1 receptors (DR1) activation on the apoptosis of osteosarcoma cells (OS732).The DR1 agonist SKF-38393 decreased the viability of OS732 cells and increased their rate of apoptosis, whereas the DR1 antagonist SCH-23390 abolished the effects of SKF-38393. In OS732 cells, overexpression of DR1 increased the rate of apoptosis, caspase-9 and -3 expression, and the release of cytochrome c (Cyt c), reduced Bcl-2 expression, inhibited extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation, and induced phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) and c-Jun N-terminal kinase (JNK). Read More

    Investigation of morin-induced insulin secretion in cultured pancreatic cells.
    Clin Exp Pharmacol Physiol 2017 Jul 12. Epub 2017 Jul 12.
    Department of Food Science, College of Agriculture, National Pingtung University of Science and Technology, Pingtung, Taiwan.
    Morin is a flavonoid contained in guava that is known to reduce hyperglycemia in diabetes. Insulin secretion has been demonstrated to increase following the administration of morin. The present study is designed to investigate the potential mechanism(s) of morin-induced insulin secretion in the MIN6 cell line. Read More

    (Pro)renin receptor activation increases profibrotic markers and fibroblast-like phenotype through MAPK-dependent ROS formation in mouse renal collecting duct cells.
    Clin Exp Pharmacol Physiol 2017 Jul 11. Epub 2017 Jul 11.
    Department of Physiology, School of Medicine, Tulane University, New Orleans, LA, USA.
    Recent studies suggested that activation of the PRR upregulates profibrotic markers through reactive oxygen species (ROS) formation; however, the exact mechanisms have not been investigated in CD cells. We hypothesized that activation of the PRR increases the expression of profibrotic markers through MAPK-dependent ROS formation in CD cells. Mouse renal CD cell line (M-1) was treated with recombinant prorenin plus ROS or MAPK inhibitors and PRR-shRNA to evaluate their effect on the expression of profibrotic markers. Read More

    AQP4-knockout aggravation of isoprenaline-induced myocardial injury is mediated by p66Shc and endoplasmic reticulum stress.
    Clin Exp Pharmacol Physiol 2017 Jul 8. Epub 2017 Jul 8.
    Institute of Nephrology, Medical School of Southeast University, Nanjing, China.
    Aquaporin 4 (AQP4) is a type of water channel protein that maintains the water balance of cardiomyocytes. However, the physiological role of AQP4 in cardiovascular disease is poorly understood. We wanted to explore whether p66Shc and endoplasmic reticulum stress participates in AQP4 knockout (KO)-mediated cardiac injury. Read More

    Adipose-derived mesenchymal stem cells therapy for acute kidney injury induced by ischemia-reperfusion in a rat model.
    Clin Exp Pharmacol Physiol 2017 Jul 8. Epub 2017 Jul 8.
    Department of Anesthesiology, Zhujiang Hospital of Southern Medical University, Guangzhou, China.
    Acute kidney injury (AKI) represents a group of complicated syndromes with a high mortality rate. The administration of adipose-derived mesenchymal stem cells (ADMSCs) has been tested as a possible treatment method for AKI. The long-term evaluation of AKI induced by ischemia/reperfusion (IR) and the probable renal protection of ADMSCs are limited. Read More

    Distribution of CYP2C8 and CYP2C9 amino acid substitution alleles in South Indian diabetes patients: A genotypic and computational protein phenotype study.
    Clin Exp Pharmacol Physiol 2017 Jul 7. Epub 2017 Jul 7.
    Department of Biotechnology, KL University, Vaddeswaram, Andhra Pradesh, India.
    The CYP2C8 and CYP2C9 are two major isoforms of the cytochrome P450 enzyme family, which is involved in drug response, detoxification, and disease development. This study describes the differential distribution of amino acid substitution variants of CYP2C8 (*2-I269F & *3-R139K) and CYP2C9 (*2-C144R & *3-L359A) genes in 234 type 2 diabetes mellitus (T2DM) patients and 218 healthy controls from Andhra Pradesh, South India. Single locus genotype analysis has revealed that homozygous recessive genotypes of 2C8*2-TT (P ≤ . Read More

    Hypertonic saline inhibits airway smooth muscle contraction by inhibiting Ca(2+) sensitization.
    Clin Exp Pharmacol Physiol 2017 Oct 24;44(10):1053-1059. Epub 2017 Aug 24.
    Institute for Medical Biology and Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan, Hubei, China.
    The effects of hypertonic solution on airway smooth muscle (ASM) contraction and the underlying mechanisms are largely unknown. We found that hypertonic saline (HS) inhibited acetylcholine (ACh)-induced contraction of ASM from the mouse trachea and human bronchi. In single mouse ASM cells (ASMCs), ACh induced an increase in intracellular Ca(2+) that was further enhanced by 5% NaCl, indicating that the HS-induced inhibition of ASM contraction was not mediated by a decrease in cytosolic Ca(2+) . Read More

    Role of peripheral vascular resistance as an indicator of cardiovascular abnormalities in patients with Parkinson's disease.
    Clin Exp Pharmacol Physiol 2017 Jul 5. Epub 2017 Jul 5.
    Department of Hygiene, Epidemiology and Ergonomy, Faculty of Health Sciences, Ludwik Rydygier Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Torun, Bydgoszcz, Poland.
    The aim of this study was to evaluate cardiovascular autonomic modulation in response to an orthostatic stress in healthy subjects and Parkinson's disease (PD). The study included 47 controls and 56 PD patients divided into groups (vasoconstrictor PD, vasodilator PD, control) according to vasodilation/vasoconstriction response during 70° head up tilt test. Using impedance cardiography (ICG) and electrocardiography (ECG) we measured stroke volume, cardiac output, left ventricular work index, left ventricular ejection time, acceleration index, index of contractility, Heather index, thoracic fluid content, total peripheral resistance, total arterial compliance. Read More

    Antitumor bioactive peptides isolated from marine organisms.
    Clin Exp Pharmacol Physiol 2017 Jul 4. Epub 2017 Jul 4.
    Department of Medical Oncology, Sir Run Run Shaw Hospital Affiliated to Zhejiang University, School of Medicine, Hangzhou, 310019, China.
    Marine organisms are an important source of antitumor active substances. Thus, pharmaceutical research in recent years has focused on exploring new antitumor drugs derived from marine organisms, and, many peptide drugs with strong antitumor activities have been successfully extracted. Based on different mechanisms, this paper reviews the research on several typical antitumor bioactive peptides in marine drugs and the latest progress therein. Read More

    Down-regulation of long non-coding RNA MALAT1 by RNA interference inhibits proliferation and induces apoptosis in multiple myeloma.
    Clin Exp Pharmacol Physiol 2017 Oct 24;44(10):1032-1041. Epub 2017 Aug 24.
    Department of Hematology, Affiliated Shengjing Hospital of China Medical University, Shenyang, Liaoning Province, China.
    Multiple myeloma (MM) is a neoplastic plasma-cell disorder characterized by abnormal proliferation of monoclonal plasma cells in the bone marrow. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), an evolutionarily highly conserved long non-coding RNA was originally identified in metastatic non-small cell lung cancer and has been reported to be up-regulated in many other cancers. However, the function of MALAT1 in MM remains unknown. Read More

    Salinomycin overcomes acquired tamoxifen resistance through AIB1 and inhibits cancer cell invasion in endocrine resistant breast cancer.
    Clin Exp Pharmacol Physiol 2017 Oct 24;44(10):1042-1052. Epub 2017 Aug 24.
    Department of Pharmacology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
    Salinomycin is a monocarboxylic polyether ionophore isolated from Streptomyces albus. It has been widely used as an antibiotic in veterinary medicine in poultry. A recent study demonstrated that salinomycin selectively inhibits human breast cancer stem cells; one possible mechanism of tamoxifen resistance. Read More

    Lower muscle tissue is associated with higher pulse wave velocity: A systematic review and meta-analysis of observational study data.
    Clin Exp Pharmacol Physiol 2017 Oct 24;44(10):980-992. Epub 2017 Aug 24.
    Bone and Muscle Health Research Group, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Australia.
    Muscle loss and arterial stiffness share common risk factors and are commonly seen in the elderly. We aimed to synthesise the existing literature on studies that have examined this association. We searched electronic databases for studies reporting correlations or associations between a measure of muscle tissue and a measure of arterial stiffness. Read More

    The use of antioxidant agents for chemotherapy-induced peripheral neuropathy treatment in animal models.
    Clin Exp Pharmacol Physiol 2017 Oct 24;44(10):971-979. Epub 2017 Aug 24.
    Federal University of Sergipe, Department of Pharmacy, Lagarto, Sergipe, Brazil.
    Antineoplastic drugs such as cisplatin, oxaliplatin, paclitaxel and vincristin are widely used in the treatment of several solid and blood tumours. However, the severity of peripheral neuropathy caused by these agents can affect the patient's quality of life. The major symptoms of chemotherapy-induced peripheral neuropathy (CIPN) involve: sensory loss, paresthesia, dysesthaesia, numbness, tingling, temperature sensitivity, allodynia and hyperalgesia, in a "stocking and glove" distribution. Read More

    Ferulic acid alleviates symptoms of preeclampsia in rats by upregulating vascular endothelial growth factor.
    Clin Exp Pharmacol Physiol 2017 Oct 24;44(10):1026-1031. Epub 2017 Aug 24.
    Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
    Preeclampsia is a complication affecting pregnant women worldwide, which leads to maternal and fetal morbidity and mortality. In this study, we evaluated the efficacy of ferulic acid (FA) on an N(ω) -nitro-L-arginine methyl ester hydrochloride (L-NAME) induced rat model of preeclampsia. L-NAME was administered to pregnant rats to induce preeclampsia. Read More

    Targeting protein for Xenopus kinesin-like protein 2 knockdown enhances radiation sensitivity of human lung squamous carcinoma cell.
    Clin Exp Pharmacol Physiol 2017 Oct 24;44(10):1060-1068. Epub 2017 Aug 24.
    Department of Radiotherapy, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
    The targeting protein for Xenopus kinesin-like protein 2 (TPX2) has been demonstrated to be associated with the tumourigenesis of many cancers. In the present study, we investigated the role and preliminary mechanism of TPX2 in the resistance of lung squamous carcinoma to radiation therapy. The results showed that SK-MES-1R and NCI-H226R cells were more resistant to X-ray irradiation than the parental cells (SK-MES-1 and NCI-H226). Read More

    A co-drug conjugate of naringenin and lipoic acid mediates neuroprotection in a rat model of oxidative stress.
    Clin Exp Pharmacol Physiol 2017 Oct 24;44(10):1008-1016. Epub 2017 Aug 24.
    Department of Biomedicinal Chemistry, Mokwon University, Daejeon, Korea.
    Using our in vitro and in vivo models of oxidative stress, the current study was designed to determine the neuroprotective potential of naringenin, alone or in combination with lipoic acid. In our mixed neuronal culture exposed to hypoxia and subsequent reoxygenation, naringenin was shown to provide significant neuroprotection against cell death at a concentration of 2.5 μmol/L. Read More

    Opposite actions of urotensin II and relaxin-2 on cellular expression of fibronectin in renal fibrosis: A preliminary experimental study.
    Clin Exp Pharmacol Physiol 2017 Oct 24;44(10):1069-1071. Epub 2017 Aug 24.
    Chair of Nephrology, Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy.
    Our aim was to evaluate the role of urotensin II, urantide (urotensin II receptor antagonist) and relaxin-2 on the cellular expression of fibronectin as a surrogate marker for renal fibrosis. We employed LLC-PK1 renal tubular epithelial cells and assessed the influence on the fibrotic process of the above-mentioned substances by using anti-fibronectin antibodies in western blot analysis. The addition of urotensin II increased fibronectin expression. Read More

    Immunosuppressive efficacy of tetrandrine combined with methylprednisolone against mitogen-activated peripheral blood mononuclear cells of haemodialysis patients.
    Clin Exp Pharmacol Physiol 2017 Sep;44(9):924-931
    Department of Clinical Pharmacology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Japan.
    Immunosuppressive therapy for prevention of acute rejection episode occasionally causes serious adverse effects, and thus it is important to develop new therapeutic approach for renal transplant recipients. This study evaluated the immunosuppressive pharmacodynamics of tetrandrine (TET) and/or methylprednisolone (MP) in haemodialysis patients in vitro by using the peripheral blood mononuclear cells (PBMCs) isolated from whole blood of haemodialysis patients. The median (range) of MP IC50 values against the proliferation of patients PBMCs was 7. Read More

    Effect of iloprost on biomarkers in patients with congenital heart disease-pulmonary arterial hypertension.
    Clin Exp Pharmacol Physiol 2017 Sep;44(9):914-923
    Department of Pharmacy, Zhongshan Hospital, Fudan University, Shanghai, China.
    Some biomarkers play important roles in the endothelial dysfunction of patients with pulmonary arterial hypertension (PAH), including nitric oxide (NO), endothelin-1 (ET-1), asymmetric dimethylarginine (ADMA), galectin-3 (Gal-3), B-type natriuretic peptide (BNP), and uric acid (UA). However, studies on these biomarkers in pulmonary artery blood in congenital heart disease-PAH (CHD-PAH) and the effect of iloprost on the regulation of biomarkers are lacking. This study investigated potential CHD-PAH biomarkers and their association with the severity of disease. Read More

    Long non-coding RNAs in aging organs and tissues.
    Clin Exp Pharmacol Physiol 2017 Jun 11. Epub 2017 Jun 11.
    Geriatrics Institute of Zhejiang Province & Zhejiang Provincial Key Lab of Geriatrics, Zhejiang Hospital, Hangzhou, 310013, PR China.
    The aging process directly impacts on bodily functions on multiple levels, including a reduced ability to resist stress, damage and disease. Besides changes in metabolic control, the aging process coincides with the altered long non-coding RNAs (lncRNAs) expression, which are ≧200nt long class of non-protein coding RNAs. The majority of non-coding transcripts of mammalian organs and tissues are expressed in developmentally regulated and cell-type specific manners. Read More

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