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    2117 results match your criteria Clinical Hemorheology and Microcirculation [Journal]

    1 OF 43

    The effect of three different surgical techniques for colon anastomosis on regional postoperative microperfusion: Laser Doppler Flowmetry study in pigs.
    Clin Hemorheol Microcirc 2018 ;68(1):61-70
    Academic Department of Surgery, Medical Faculty, Charles University and Teaching Hospital, Hradec Kralove, Czech Republic.
    Background: The optimal surgical approach to reconnecting bowel ends safely after resection is of great importance.

    Objectives: This project is focused on assessment of the perianastomotic microcirculation quality in the short postoperative period when using three different anastomosis techniques in experimental animal.

    Methods: The experimental study involved 27 young female domestic pigs divided into three subgroups of 9 animals according to each surgical method of anastomosis construction in the sigmoid colon region: by manual suture, by stapler, or by gluing. Read More

    Lumbar sympathectomy reduces vascular permeability, possibly through decreased adenosine receptor A2a expression in the hind plantar skin of rats.
    Clin Hemorheol Microcirc 2018 ;68(1):5-15
    Southern Medical University, Guangzhou, China.
    Background: The effect of lumbar sympathectomy for the treatment of lower limb ischemia remains a matter of controversy.

    Methods: Sprague-Dawley rats were subjected to lumbar sympathectomy, after which Evans blue dye was injected into the hind plantar skin. Extravasation of dye was measured and compared with rats undergoing sham operation. Read More

    Noninvasive quantitative assessment of microcirculatory disorders of the upper extremities with 2D fluorescence optical imaging.
    Clin Hemorheol Microcirc 2018 Feb 2. Epub 2018 Feb 2.
    Department of Diagnostic and Interventional Radiology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
    Background: Quantitative Imaging of microcirculatory disorders is challenging.

    Objective: To investigate the feasibility of 2D Fluorescence Optical Imaging (FOI) for characterization and quantification of microcirculatory disorders in peripheral arterial occlusive disease (PAOD) of the upper extremity.

    Methods: 9 patients with various clinical presentations of PAOD of the upper extremity were included. Read More

    Chances and limitations of isolated mouse heart models for investigating the endothelial glycocalyx1.
    Clin Hemorheol Microcirc 2018 Feb 2. Epub 2018 Feb 2.
    Department of Anaesthesiology, University Hospital of Munich (LMU), Munich, Germany.
    Background: The endothelial glycocalyx plays a decisive role in maintaining vascular homeostasis. Previous animal models have mainly focused on in-vitro experiments or the isolated beating guinea pig heart. To further evaluate underlying mechanisms of up- and down regulation, knock-out animals seem to be a promising option. Read More

    Microhemodynamic indices to evaluate the effectiveness of herbal medicine in diabetes: A comparison between alpha-mangostin and curcumin in the retina of type 2 diabetic rats.
    Clin Hemorheol Microcirc 2018 Jan 10. Epub 2018 Jan 10.
    National Cardiovascular Center Research Institute, Osaka, Japan.
    Objective: This study was aimed to develop microhemodynamic indices to evaluate the effectiveness of herbal medicine in diabetic tissues.

    Methods: Male Sprague-Dawley rats were divided into four groups: normal control rats (Control), type 2 diabetic rats without (DM2) and with supplementation of alpha mangostin (DM2-MG) or curcumin (DM2-CUR). Alpha-mangostin or curcumin (200 mg/kg BW) were fed followed by i. Read More

    Rheopheresis as a causal therapy option for systemic scleroderma (SSc).
    Clin Hemorheol Microcirc 2017 ;67(3-4):229-240
    Department of Dermatology, University Medicine Greifswald, Greifswald, Germany.
    A complex pathomechanism accounts systemic sclerosis as a form of collagenosis. A triad of vasculopathy, autoinflammation, and dysbalance of the fibroblast function can be seen as cause, as well as symptomatic appearance. Comparative with other collagenoses, e. Read More

    Extracellular ATP activates eNOS and increases intracellular NO generation in Red Blood Cells.
    Clin Hemorheol Microcirc 2018 ;68(1):89-101
    Department of Physiology, Medical Faculty, Akdeniz University, Antalya, Turkey.
    Background: It has been well documented that ATP activates NOS enzymes and causes increased NO production in several cell types. Although RBC known to possesses eNOS enzyme activity, it has not been investigated whether RBC eNOS could be induced by extracellular ATP.

    Objective: The aim of the present study is to evaluate extracellular ATP mediated eNOS activation and NO production in RBC. Read More

    Pharmacosimulation of interruptions and its solution in intravenous administration of cangrelor.
    Clin Hemorheol Microcirc 2017 Oct 7. Epub 2017 Oct 7.
    Department of Internal Medicine, Division of Cardiology, Pulmonary Diseases, Vascular Medicine, University Hospital Düsseldorf, Düsseldorf, Germany.
    BACKGROUNDCangrelor is an intravenous adenosine diphosphate (ADP) P2Y12 receptor antagonist, which has to be administered as a bolus followed by immediate infusion. Nevertheless, in clinical routine deviations from the correct practice, such as delayed infusion onset or interruptions during infusion, may occur.

    Objective: The objective of the present study was to investigate the impact of administration delays on cangrelor concentration in a pharmacological simulation setting and to give possible solutions for the clinical practice. Read More

    Misinterpretation of the sublingual microcirculation during therapy with levosimendan.
    Clin Hemorheol Microcirc 2018 ;68(1):83-87
    Department of Anaesthesiology, Intensive Care and Pain Medicine, Münster, NRW, Germany.
    There is increasing evidence in the literature that preoperative treatment with levosimendan optimizes cardiopulmonary haemodynamics in patients scheduled for the implantation of a Left Ventricular Assist Device (LVAD). The present case report describes changes in sublingual microcirculation using incident dark field video microscopy in a patient, who received a continuous infusion of 0.5 mg/h levosimendan 12 h before LVAD implantation. Read More

    Quantitative parameters of contrast-enhanced ultrasonography for assessment of renal pathology: A preliminary study in chronic kidney disease.
    Clin Hemorheol Microcirc 2018 ;68(1):71-82
    Department of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
    Objective: To assess the severity of renal pathology in patients with chronic kidney disease (CKD) using contrast-enhanced ultrasonography (US).

    Methods: 275 patients with CKD who were proven by renal biopsy and 30 healthy adults were examined using conventional US and contrast-enhanced US. Ultrasonic parameters included renal length, cortical thickness, rise time (RT), peak intensity (PI), area under the time-intensity curve (AUC), wash-in slope (WIS) and time to peak (TTP). Read More

    Hemorheological parameters in Monoclonal Gammopathy of Undetermined Significance (MGUS).
    Clin Hemorheol Microcirc 2018 ;68(1):51-59
    Dipartimento Biomedico di Medicina Interna e Specialistica, Università di Palermo, Palermo, Italy.
    There is scarcity of information about the hemorheological pattern in subjects with Monoclonal Gammopathy of Undetermined Significance (MGUS). This preliminary research is focused on the behaviour of whole-blood and plasma viscosity, haematocrit and erythrocyte deformability in the above clinical condition. We enrolled 21 MGUS subjects (10 women and 11 men; mean age 66. Read More

    No effect on the short-term of a decrease in blood viscosity on insulin resistance.
    Clin Hemorheol Microcirc 2018 ;68(1):45-50
    Department of Health Science, Magna Græcia University of Catanzaro, Catanzaro, Italy.
    Background: Blood viscosity (BV) might influence glucose delivery to peripheral tissues and play an important role in insulin resistance and diabetes mellitus. However, the exact relationship between BV and insulin resistance is not yet clear.

    Objectives: Aim of the present study is to evaluate the effects of the acute reduction in BV on insulin resistance, in healthy male subjects. Read More

    Effects of venotonic drugs on the microcirculation: Comparison between Ruscus extract and micronized diosmine1.
    Clin Hemorheol Microcirc 2017 Oct 7. Epub 2017 Oct 7.
    Laboratory for Clinical and Experimental Research on Vascular Biology, Biomedical Center, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
    Background: Chronic venous disease of the lower limbs is a common public health problem worldwide with negative impact on quality of life and results with drugs used to treat it are sparse, probably due to lack of good experimental models.

    Objective: In this investigation we have tested the effects of two commonly used venotonic substances, Ruscus extract and micronized diosmine, on the microcirculation in vivo.

    Methods: These substances were given orally, by gavage, during two weeks, twice daily and observations were made using the hamster cheek pouch preparation. Read More

    Intestinal ischemia-reperfusion leads to early systemic micro-rheological and multiorgan microcirculatory alterations in the rat.
    Clin Hemorheol Microcirc 2018 ;68(1):35-44
    Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Hungary.
    Background: Intestinal ischemia-reperfusion (I/R) is a potentially life-threatening situation and its pathomechanism is not fully understood yet.

    Objective: To investigate the early micro-rheological, microcirculatory and morphological consequences of intestinal I/R in a rat model.

    Methods: CD rats were anesthetized and subjected to Control (n = 7) or I/R (n = 7) groups. Read More

    Erythrocyte deformability and hemorheological profile in multiple myeloma.
    Clin Hemorheol Microcirc 2018 ;68(1):25-34
    Dipartimento Biomedico di Medicina Interna e Specialistica, Università degli Studi di Palermo, Palermo, Italy.
    The hemorheological profile in multiple myeloma (MM) has been extensively studied. Our investigation regarded the behavior of whole-blood viscosity, plasma viscosity and erythrocyte deformability in MM. We enrolled 24 MM patients; 13 of them had been recently diagnosed and were at the initial stage of therapy, 6 were on consolidation/conservation therapy and 5 had achieved a complete remission. Read More

    Assessing the effect of different operation techniques on postoperative duplex ultrasound quality after carotid endarterectomy.
    Clin Hemorheol Microcirc 2018 ;68(1):17-24
    Department of General, Thoracic, Vascular and Transplantation Surgery, University Medicine Rostock, Germany.
    Background: Duplex ultrasound is the first choice in diagnostics and surveillance of stenoses of the internal carotid arteries before and even after surgery. Therefore, the quality of duplex ultrasound is crucial to investigate these vascular pathologies.

    Objective: Aim of this study was the evaluation whether different surgical techniques affect the postoperative quality of duplex ultrasound. Read More

    Clinical relevance of early sublingual microcirculation monitoring in septic shock patients.
    Clin Hemorheol Microcirc 2017 Oct 7. Epub 2017 Oct 7.
    Department of Anaesthesia, Pain Management and Perioperative Medicine's, Faculty of Medicine, Dalhousie University, Halifax, Canada.
    Introduction: Although microcirculation dysfunction plays unique role in septic shock, translation of microcirculation to clinical practices is limited by current semi-quantities analysis and unclear clinical relevance of microcirculation monitoring. Our aim was to critically evaluate the characteristic nature and relevant clinical important of microcirculation.

    Evidence Acquisition: Pubmed (2000 to August 2015) were searched to identify observation, case-control, intervention and randomized clinical studies evaluating the relationship between microcirculation alterations and mortality, morbidity and drug responses. Read More

    Glycocalyx in vivo measurement.
    Clin Hemorheol Microcirc 2017 ;67(3-4):499-503
    Department of Anaesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada.
    The endothelial glycocalyx (EG) lining the endoluminal surface of the capillaries has been proposed as a key component of the microcirculation and a major player in microvascular pathology. Recent advances in the understanding of its physiological role and clinical significance have been made upon the development of methods allowing EG assessment in clinical medicine. Laboratory methods can assess the amount of EG damage by measuring levels of its degradation products (e. Read More

    Microcirculation research in community hospitals - challenges and chances.
    Clin Hemorheol Microcirc 2017 ;67(3-4):511-514
    Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, Canada.
    Community hospitals provide ideal conditions for large clinical studies because of the high volume of unselected patients admitted every year. With regard to microcirculatory studies, there are still some feasibility problems which are not solved yet. First of all, the lack of reliable automated software to analyze microcirculatory images represents the most important issue. Read More

    Preliminary clinical evaluation of automated analysis of the sublingual microcirculation in the assessment of patients with septic shock: Comparison of automated versus semi-automated software.
    Clin Hemorheol Microcirc 2017 ;67(3-4):489-498
    Department of Anaesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, Nova Scotia, Canada.
    Introduction: The outcome of patients in septic shock has been shown to be related to changes within the microcirculation. Modern imaging technologies are available to generate high resolution video recordings of the microcirculation in humans. However, evaluation of the microcirculation is not yet implemented in the routine clinical monitoring of critically ill patients. Read More

    Short-term hypoxia and vasa recta function in kidney slices.
    Clin Hemorheol Microcirc 2017 ;67(3-4):475-484
    Institute of Vegetative Physiology, Charité-Universitätsmedizin Berlin, Germany.
    Background: Descending vasa recta (DVR) supply the inner part of outer renal medulla an area at risk for hypoxic damages.

    Objective: We hypothesize increased vasoreactivity after hypoxia/re-oxygenation (H/R) in DVR, which might contribute to the reduced medullary perfusion after an ischemic event.

    Methods: Live kidney slices (200μm) from SD rats were used for functional experiments. Read More

    Leukocyte-reduced platelet-rich plasma stimulates the in vitro proliferation of adipose-tissue derived mesenchymal stem cells depending on PDGF signaling.
    Clin Hemorheol Microcirc 2017 ;67(2):183-196
    Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany.
    Background: Platelet-rich Plasma (PRP) is suggested as xenoprotein-free cell-culture medium replacement for animal-derived supplements.

    Objective: The aim of this study was to investigate PRP-triggered signaling in adipose derived mesenchymal stem cells (ASCs).

    Methods: PRP was obtained from 4 male patients. Read More

    Reduced amputation rate with isovolemic hemodilution in critical limb ischemia patients.
    Clin Hemorheol Microcirc 2017 ;67(2):197-208
    Department of Mechanical Engineering and Mechanics, Drexel University, Philadelphia, PA, USA.
    Aims: Critical limb ischemia (CLI) patients are characterized by intractable pain in spite of medication, non-healing ulcers, and gangrene. The objective of this study was to investigate whether or not isovolemic hemodilution treatment can reduce the rate of major amputations in CLI.

    Methods: 28 patients were studied who had tissue loss on Rutherford Grade III, Category 5 or 6. Read More

    Microcirculation measurements: Barriers for use in clinical routine.
    Clin Hemorheol Microcirc 2017 ;67(3-4):505-509
    Department of Anesthesiology, Surgical Intensive Care, Emergency and Pain Medicine, Ruhr-University Bochum, Klinikum Herford, Herford, Germany.
    Background: In patients with shock, inflammation and sepsis alterations in microcirculation are common problems. Although the pathophysiologic consequences are well understood, measurements of microcirculation have not entered clinical routine so far.

    Objective: To characterize the requirements for clinical microcirculation measurement techniques and the barriers for implementation into routine practice. Read More

    Hyperspectral imaging as a novel diagnostic tool in microcirculation of wounds.
    Clin Hemorheol Microcirc 2017 ;67(3-4):467-474
    Department of Dermatology of the University Medicine Greifswald, Sauerbruchstrasse, Greifswald, Germany.
    Monitoring of perfusion is a cornerstone in surgery, phlebology and basic science to proof wound healing by interventions. In chronic wound management it is of utmost importance to realize and parametrize wound bed perfusion to verify actual, and plan further treatment by noninvasive diagnostics. Up to now monitoring is based on visual inspection of wounds as conventionally practiced over more than decades. Read More

    Breast sensitivity after mastectomy and autologous reconstruction.
    Clin Hemorheol Microcirc 2017 ;67(3-4):459-465
    Center of Plastic and Reconstructive Surgery, University Medical Center Regensburg, Germany.
    Background: Breast Carcinoma is the most common malign tumor disease in women. 20-30% of these tumors have to be treated by removing all of the breast tissue. After that kind of therapeutic procedure, most of the women are severely traumatized and ask for reconstruction. Read More

    Presentation of a variation of the chorioallantoic membrane set up as a potential model for individual therapy for squamous cell carcinoma of the oropharynx.
    Clin Hemorheol Microcirc 2017 ;67(3-4):453-457
    Department of Plastic, Hand, and Reconstructive Surgery, University Medical Center Regensburg, Regensburg, Germany.
    The chorioallantoic membrane of fertilized chicken eggs in an early phase of breeding presents an approved test situation for the growth and treatment of human cancer cells.These models work due to the inoculation of cells into the membrane that stays within the egg shell during the time of investigation. In this study a modification of this model is presented. Read More

    Comparison of computed tomography (CT) and contrast-enhanced ultrasound (CEUS) for the quantitative evaluation of an ablation defect following radiofrequency ablation of malignant liver lesions.
    Clin Hemorheol Microcirc 2017 ;67(3-4):445-451
    Department of Radiology, University Medical Center Regensburg, Regensburg, Germany.
    Objective: To compare contrast-enhanced computed tomography (CT) and contrast-enhanced ultrasound (CEUS) for the quantitative evaluation of an ablation defect following radiofrequency ablation of malignant liver lesions in a prospective study.

    Materials And Methods: 22 radiofrequency ablations of malignant liver tumors were performed. The ablation defects were then measured and evaluated by two independent examiners via contrast-enhanced computed tomography (CT) and contrast-enhanced ultrasound (CEUS) using a 1-5 MHz multifrequency convex ultrasound transducer (LOGIQ E9, GE) with respect to the short-axis diameter, the long-axis diameter, and the area. Read More

    Postoperative control of functional muscle flaps for facial palsy reconstruction: Ultrasound guided tissue monitoring using contrast enhanced ultrasound (CEUS) and ultrasound elastography.
    Clin Hemorheol Microcirc 2017 ;67(3-4):435-444
    Department of Radiology, University Medical Center Regensburg, Germany.
    Background And Objectives: Facial paralysis causes excruciating impairments including facial asymmetry, limited eye closure, oral incontinence and social dysfunction. Modern plastic surgical reconstructions render favorable results with well-perfused dynamic muscle flaps. Post-operative tissue monitoring is a critical determinant for success. Read More

    Does Virtual Touch IQ elastography help to improve the preoperative diagnosis of parotid tumors: A prospective trial.
    Clin Hemorheol Microcirc 2017 ;67(3-4):425-434
    Institute of Radiology, Ludwig-Maximilians-Universität München, Munich, Germany.
    Background: It is difficult to assess tumors of the parotid gland preoperatively. However, it is essential for the surgeon to know which kind of tumor is present. Ultrasound is the method of choice, but there is still no reliable differential diagnostic tool for determining whether a tumor is malignant or benign. Read More

    Investigation of the acute plantar fasciitis with contrast-enhanced ultrasound and shear wave elastography - first results.
    Clin Hemorheol Microcirc 2017 ;67(3-4):415-423
    Department of Radiology and Interdisciplinary Ultrasound, University Hospital Regensburg, Regensburg, Germany.
    Background: The plantar fasciitis is a common disease with a high prevalence in public and a frequent cause of heel pain.

    Objective: In our pilot study, we wanted to characterise the feasibility of shear-wave elastography and contrast-enhanced ultrasound (CEUS) in the assessment of the plantar fasciitis.

    Methods: 23 cases of painful heels were examined by B-Mode ultrasound, Power Doppler (PD), shear wave elastography and contrast-enhanced ultrasound before anti-inflammatory radiation. Read More

    The diagnostic value of contrast-enhanced ultrasound (CEUS) as a new technique for imaging of vascular complications in renal transplants compared to standard imaging modalities.
    Clin Hemorheol Microcirc 2017 ;67(3-4):407-413
    Department of Radiology, Ludwig-Maximilians-University Munich - Grosshadern Campus, Munich, Germany.
    Background: Vascular complications in renal transplant patients are a well-known issue in post transplant patient care. If malfunctioning of the renal transplant is suspected to be caused by vascular complications an early diagnosis and therapy is required to maintain the renal transplant. Computed tomography (CT), digital substraction angiography (DSA) and radioisotope renography are the gold standard imaging modalities to diagnose vascular complications. Read More

    Effect of irreversible electroporation of prostate cancer on microcirculation: Imaging findings in contrast-enhanced T1-weighted 3D MRI.
    Clin Hemorheol Microcirc 2017 ;67(3-4):399-405
    Department of Radiology, University Medical Center Regensburg, Germany.
    Objectives: Irreversible electroporation (IRE) is a novel focal ablation technique applicable for treatment of prostate cancer (PCa). We aim to evaluate imaging findings of T1-weighted contrast-enhanced MRI after percutaneous IRE of low-risk PCa.

    Methods: A total of 13 male patients underwent IRE of focal low-risk PCa and were included in this analysis. Read More

    Ultrasound elastography in diagnosis and follow-up for patients with chronic recurrent parotitis.
    Clin Hemorheol Microcirc 2017 ;67(3-4):389-397
    Institute of Radiology, Ludwig-Maximilians-Universität München, Munich, Germany.
    Purpose: Chronic recurrent parotitis (CRP) is a non-obstructive disease with episodes characterized by painful swelling of the parotid gland. It presents in both a juvenile and an adult form, with no clear information on its actual origin. Diagnosis is based on patient medical history and ultrasound examination but is frequently not correctly identified. Read More

    Reduced microperfusion due to portal vein thrombosis: Impact on the outcome of percutaneous thermal tumor ablation.
    Clin Hemorheol Microcirc 2017 ;67(3-4):383-388
    Department of Radiology, University Hospital Regensburg, Regensburg, Germany.
    Purpose: To determine the influence of pre-interventionally existing portal vein thrombosis on the ablation success of percutaneous tumor ablation of HCC.

    Materials And Methods: 15 patients with HCC and pre-existing portal vein thrombosis underwent thermal tumor ablation. We retrospectively analyzed the pre- and post-interventionally performed CT and MRI scans in terms of technical success as well as the complication rate. Read More

    Quantitative evaluation of real-time maximum liver capacity (LiMAx) and time intensity curve (TIC) analysis in CEUS-based microperfusion.
    Clin Hemorheol Microcirc 2017 ;67(3-4):373-382
    Department of Radiology, University Hospital Regensburg, Regensburg, Germany.
    Purpose: To compare the diagnostic performance of real-time maximum liver capacity (LiMAx) with dynamic contrast-enhanced ultrasound (CEUS)-based liver microcirculation.

    Materials And Methods: 23 patients underwent liver function capacity (LiMAx) test and consecutive or previous CEUS examinations. A bolus injection of 1. Read More

    Vascular malformations of upper and lower extremity - from radiological interventional therapy to surgical soft tissue reconstruction - an interdisciplinary treatment.
    Clin Hemorheol Microcirc 2017 ;67(3-4):355-372
    Center of Plastic, Hand and reconstructive Surgery, University Hospital Regensburg, Germany.
    This article presents our experience in managing peripheral vascular malformations of upper and lower extremities over a 4-year period in a series of 46 patients of the Department of Plastic Surgery treated in the Interdisciplinary Center of Vascular Anomalies (ICVA) at the University of Regensburg. The patients presented vascular malformations of upper and lower extremity and were selected from our prospective vascular anomalies file archive from 2012 to 2016. During this period in the ICVA at University of Regensburg were performed more than 1400 radiological interventional treatments in patients with vascular malformations. Read More

    Reference range and variability of Laser-Doppler-Fluxmetry.
    Clin Hemorheol Microcirc 2017 ;67(3-4):347-353
    Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
    The Laser Doppler technique (Laser-Doppler-Fluxmetry, LDF), a noninvasive method to estimate skin blood flow (LDF), is frequently used in research and clinical routine [1]. Here, the measurements were carried out with a new Laser Doppler system, the DOP-system, which allows to measure frequency spectra in four different frequency windows according to the velocities in venules (low velocity), capillaries (low to medium velocities), and in arteries (with high and very high velocities). However, the diagnostic reliability or the effectiveness of the LDF has not yet been evaluated sufficiently, which is indispensable, where medical diagnostics and therapy controls are concerned. Read More

    The mechanisms of late scaffold thrombosis.
    Clin Hemorheol Microcirc 2017 ;67(3-4):343-346
    Kardiologie I, University Medical Center Mainz and Deutsches Zentrum für Herz und Kreislauf Forschung, Mainz, Germany.
    Coronary scaffolds have been recently developed to address the long-term limitations of metallic drug eluting stents. Concerns have however been expressed on the safety of these devices, with evidence of both early and late scaffold thrombosis. While early thrombosis has been associated with incomplete scaffold expansion, leading to flow disturbances, blood recirculation, and platelet activation, the pathophysiology of late events remains less understood. Read More

    Divisive influence of interleukin-1 receptor antagonist polymorphisms in melanoma patients.
    Clin Hemorheol Microcirc 2017 ;67(3-4):319-326
    Section of Plastic and Reconstructive Surgery, Department of Surgery, Yale University School of Medicine, New Haven, CT, USA.
    Objective: The interleukin-1 receptor antagonist (IL-1RA) contributes to tumor survival and progression in multiple cancer entities. IL-1RA polymorphisms influence IL-1RA expression patterns and function. A known polymorphism was correlated with clinical outcomes in melanoma patients with particularly aggressive disease. Read More

    Adipogenic differentiation of human adipose derived mesenchymal stem cells in 3D architectured gelatin based hydrogels (ArcGel).
    Clin Hemorheol Microcirc 2017 ;67(3-4):297-307
    Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
    Polymeric matrices mimicking multiple functions of the ECM are expected to enable a material induced regeneration of tissues. Here, we investigated the adipogenic differentiation of human adipose derived mesenchymal stem cells (hADSCs) in a 3D architectured gelatin based hydrogel (ArcGel) prepared from gelatin and L-lysine diisocyanate ethyl ester (LDI) in an one-step process, in which the formation of an open porous morphology and the chemical network formation were integrated. The ArcGel was designed to support adipose tissue regeneration with its 3D porous structure, high cell biocompatibility, and mechanical properties compatible with human subcutaneous adipose tissue. Read More

    Engineering of cell-laden gelatin-based microgels for cell delivery and immobilization in regenerative therapies.
    Clin Hemorheol Microcirc 2017 ;67(3-4):251-259
    Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
    Cell-based therapies often face the challenge of low cell retention and viability upon transplantation. Hence, biomaterials, which can immobilize transplanted cells, while at the same time support cell viability, are essential for successful clinical application. Noteworthy, biomaterials in the micrometer range such as microcapsules or microspheres have the advantage of a minimally invasive introduction into tissue. Read More

    Cardiac frequency and cutaneous microcirculation during and after exercising in the view of physical condition.
    Clin Hemorheol Microcirc 2017 ;67(3-4):221-227
    Furtwangen University, Villingen-Schwenningen, Germany.
    Introduction: In the field of cardiovascular diseases an ergometer test is a common diagnostical method in which a change in microcirculation can be reached. In this paper cardiac frequency and cutaneous microcirculation during and after exercising will be compared with each other.

    Materials And Methods: The cutaneous microcirculation of  6 healthy volunteers (2 females, 4 males) is measured. Read More

    Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures.
    Clin Hemorheol Microcirc 2017 ;67(3-4):327-342
    Department of Cell Biology and Tissue Engineering, Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Senftenberg, Germany.
    Background: Tetrazolium-based assays are optimized to assess proliferation/toxicity of monolayer or suspension cells in microtiter plates. With regard to tissue engineering and regenerative medicine the need for in vivo like 3D microtissues has an increasing relevance. Applying tetrazolium-based assays to 3D culture systems is technically more challenging. Read More

    Effects of Tacrolimus or Sirolimus on the adhesion of vascular wall cells: Controlled in-vitro comparison study.
    Clin Hemorheol Microcirc 2017 ;67(3-4):309-318
    Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
    In drug eluting stents the cytostatic drugs Sirolimus or Tacrolimus are used to inhibit blood vessel restenosis by limiting the proliferation of smooth muscle cells. However, the cytostatic activity of both drugs was shown to be not cell specific and could also affect the stent endothelialisation, respectively. Currently, only limited in vitro data are available about the impact of Sirolimus and Tacrolimus on endothelial cell proliferation over a broad concentration range. Read More

    Folate receptor mediated genetic modification of human mesenchymal stem cells via folic acid-polyethylenimine-grafted poly(N-3-hydroxypropyl)aspartamide.
    Clin Hemorheol Microcirc 2017 ;67(3-4):279-295
    Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
    Mesenchymal stem cells (MSCs) are targeted as vehicles for cell mediated gene therapy. Here we report on a macromolecular carrier, which was designed aiming at successful targeted gene delivery into MSCs through the mediation of folate receptor and reduced cytotoxicity compared to established cationic polymer vector - polyethylenimine with a weight average molecular weight (Mw) of 25,000 Dalton (PEI25K). The carrier PHPA-PEI1800-FA was synthesized in a two-step procedure. Read More

    Modulation of the mesenchymal stem cell migration capacity via preconditioning with topographic microstructure.
    Clin Hemorheol Microcirc 2017 ;67(3-4):267-278
    Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
    Controlling mesenchymal stem cells (MSCs) behavior is necessary to fully exploit their therapeutic potential. Various approaches are employed to effectively influence the migration capacity of MSCs. Here, topographic microstructures with different microscale roughness were created on polystyrene (PS) culture vessel surfaces as a feasible physical preconditioning strategy to modulate MSC migration. Read More

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