Search our Database of Scientific Publications and Authors

I’m looking for a

    5850 results match your criteria Clinical Chemistry and Laboratory Medicine CCLM [Journal]

    1 OF 117

    Osteocalcin value to identify subclinical atherosclerosis over atherosclerotic cardiovascular disease (ASCVD) risk score in middle-aged and elderly Chinese asymptomatic men.
    Clin Chem Lab Med 2018 May 19. Epub 2018 May 19.
    Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai, P.R. China.
    Background: Our study examined whether osteocalcin contributed to identifying carotid intima-media thickness (C-IMT) over the atherosclerotic cardiovascular disease (ASCVD) risk score.

    Methods: We recruited 618 middle-aged and elderly men from communities in Shanghai. Serum osteocalcin levels were determined using an electrochemiluminescence immunoassay. Read More

    Optimizing charge state distribution is a prerequisite for accurate protein biomarker quantification with LC-MS/MS, as illustrated by hepcidin measurement.
    Clin Chem Lab Med 2018 May 19. Epub 2018 May 19.
    Expert Center Clinical Chemistry Eindhoven, Eindhoven, The Netherlands.
    Background: Targeted quantification of protein biomarkers with liquid chromatography-tandem mass spectrometry (LC-MS/MS) has great potential, but is still in its infancy. Therefore, we elucidated the influence of charge state distribution and matrix effects on accurate quantification, illustrated by the peptide hormone hepcidin.

    Methods: An LC-MS/MS assay for hepcidin, developed based on existing literature, was improved by using 5 mM ammonium formate buffer as mobile phase A and as an elution solution for solid phase extraction (SPE) to optimize the charge state distribution. Read More

    Exploring the potential of mucin 13 (MUC13) as a biomarker for carcinomas and other diseases.
    Clin Chem Lab Med 2018 May 16. Epub 2018 May 16.
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
    Background: Mucin 13 (MUC13) is a cell surface glycoprotein aberrantly expressed in a variety of epithelial carcinomas. Thus far, the role of MUC13 in various diseases remains elusive. To the best of our knowledge, this is the first study to examine the potential of MUC13 as a serum biomarker in a variety of carcinomas and other conditions. Read More

    Harmonization activities of Noklus - a quality improvement organization for point-of-care laboratory examinations.
    Clin Chem Lab Med 2018 May 16. Epub 2018 May 16.
    The Norwegian Quality Improvement of Laboratory Examinations (Noklus), Haraldsplass Deaconess Hospital, Bergen, Norway.
    Noklus is a non-profit quality improvement organization that focuses to improve all elements in the total testing process. The aim is to ensure that all medical laboratory examinations are ordered, performed and interpreted correctly and in accordance with the patients' needs for investigation, treatment and follow-up. For 25 years, Noklus has focused on point-of-care (POC) testing in primary healthcare laboratories and has more than 3100 voluntary participants. Read More

    The meteoric rise and dramatic fall of Theranos: lessons learned for the diagnostic industry.
    Clin Chem Lab Med 2018 May 11. Epub 2018 May 11.
    Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, ON, Canada.
    In this piece we discuss and reflect on the conclusion of the Theranos saga in the light of its fraud conviction. Theranos (founded in 2003 by Elizabeth Holmes) was supposed to disrupt the diagnostic testing industry by developing technology which could perform dozens of tests using a tiny amount of blood from a finger-prick. As a result, Ms. Read More

    Analysis of protein glycation in human fingernail clippings with near-infrared (NIR) spectroscopy as an alternative technique for the diagnosis of diabetes mellitus.
    Clin Chem Lab Med 2018 May 11. Epub 2018 May 11.
    Department of Clinical Chemistry, Ghent University Hospital, Ghent, Belgium.
    Background: Glycated keratin allows the monitoring of average tissue glucose exposure over previous weeks. In the present study, we wanted to explore if near-infrared (NIR) spectroscopy could be used as a non-invasive diagnostic tool for assessing glycation in diabetes mellitus.

    Methods: A total of 52 patients with diabetes mellitus and 107 healthy subjects were enrolled in this study. Read More

    Trueness, precision and stability of the LIAISON 1-84 parathyroid hormone (PTH) third-generation assay: comparison to existing intact PTH assays.
    Clin Chem Lab Med 2018 May 11. Epub 2018 May 11.
    DiaSorin Inc., Stillwater, MN, USA.
    Background: Over the past few decades, parathyroid hormone (PTH) immunoassays have progressed through successive generations resulting in increased specificity and accuracy for detecting circulating PTH. With the introduction of third-generation assays, in which the biologically active PTH(1-84) is specifically targeted, the PTH(7-84) and other fragments are not detected. The specific recognition of only PTH(1-84) whole molecule allows for more reliable standardization and calibration than with the existing assays. Read More

    Harmonization of urine albumin/creatinine ratio (ACR) results: a study based on an external quality assessment program in Polish laboratories.
    Clin Chem Lab Med 2018 May 11. Epub 2018 May 11.
    Department of Clinical Chemistry, Medical University of Gdańsk, Gdańsk, Poland.
    Background: The ratio of albumin to creatinine (ACR) is an important parameter used for detection of albuminuria in patients with early kidney damage. The aim of the study was to evaluate the harmonization of ACR results among Polish participants in an international external quality assessment (EQA) program, and to evaluate the impact of albumin and creatinine analytical performance on the harmonization of ACR results.

    Methods: We analyzed 182 results of albumin, 202 of creatinine, and 180 of ACR obtained from Polish laboratories in an EQA program organized by Labquality. Read More

    Evaluation of the clinical implementation of a large-scale online e-learning program on venous blood specimen collection guideline practices.
    Clin Chem Lab Med 2018 May 11. Epub 2018 May 11.
    Department of Nursing, Umeå University, Umeå, Sweden, Phone: +46 90 786 9115.
    Background: When performed erroneously, the venous blood specimen collection (VBSC) practice steps patient identification, test request management and test tube labeling are at high risk to jeopardize patient safety. VBSC educational programs with the intention to minimize risk of harm to patients are therefore needed. In this study, we evaluate the efficiency of a large-scale online e-learning program on personnel's adherence to VBSC practices and their experience of the e-learning program. Read More

    Harmonization of units and reference intervals of plasma proteins: state of the art from an External Quality Assessment Scheme.
    Clin Chem Lab Med 2018 May 11. Epub 2018 May 11.
    Centre of Biomedical Research, University-Hospital of Padova, Padova, Italy.
    Background: The need to harmonize laboratory information is particularly intense in the field of plasma proteins, considering their clinical impact and relevance in monitoring diseases.

    Methods: We evaluated units and reference intervals (RIs) utilized by participants of the External Quality Assessment Scheme (EQAS) for plasma proteins of the Centre of Biomedical Research. Moreover, we evaluated inter-laboratory analytical variability from 2001 to 2017. Read More

    mRNA expression profile in peripheral blood mononuclear cells based on ADRB1 Ser49Gly and Arg389Gly polymorphisms in essential hypertension - a case-control pilot investigation in South Indian population.
    Clin Chem Lab Med 2018 May 11. Epub 2018 May 11.
    Food and Drug Toxicology Research Centre, National Institute of Nutrition (Indian Council of Medical Research), Hyderabad, Telangana, India.
    Background: β1-Adrenoreceptor (ADRB1) genetic polymorphisms are widely studied for susceptibility to many cardiovascular diseases such as essential hypertension. However, the mRNA expression of ADRB1 is rarely studied.

    Methods: A case-control pilot study with 292 hypertensives and 324 controls was designed to evaluate the role of the Ser49Gly and Arg389Gly, which are commonly studied single nucleotide polymorphisms (SNP), in the mRNA levels of ADRB1, in conjunction with its genetic predisposition to essential hypertension. Read More

    Establishing normal values of total testosterone in adult healthy men by the use of four immunometric methods and liquid chromatography-mass spectrometry.
    Clin Chem Lab Med 2018 May 10. Epub 2018 May 10.
    Department of Laboratory Medicine, Ente Ospedaliero Cantonale, Lugano, Switzerland.
    Background: The total testosterone (T) cutoffs clinically adopted to define late-onset hypogonadism (LOH) do not consider the differences that exist between different analytical platforms, nor do they consider the body mass index (BMI) or age of the patient. We aimed at providing method, age and BMI-specific normal values for total T in European healthy men.

    Methods: A total of 351 eugonadal healthy men were recruited, and total T was measured with four automated immunometric assays (IMAs): ARCHITECT i1000SR (Abbott), UniCel DxI800 (Beckman Coulter), Cobas e601 (Roche), IMMULITE 2000 (Siemens) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Read More

    Metrological traceability and harmonization of medical tests: a quantum leap forward is needed to keep pace with globalization and stringent IVD-regulations in the 21st century!
    Clin Chem Lab Med 2018 May 7. Epub 2018 May 7.
    University of Reims Champagne-Ardenne, Faculty of Medicine, UMR CNRS/URCA n° 7369, and University Hospital of Reims, Department of Biochemistry, Reims, France.
    In our efforts to advance the profession and practice of clinical laboratory medicine, strong coordination and collaboration are needed more than ever before. At the dawn of the 21st century, medical laboratories are facing many unmet clinical needs, a technological revolution promising a plethora of better biomarkers, financial constraints, a growing scarcity of well-trained laboratory technicians and a sharply increasing number of International Organization for Standardization guidelines and new regulations to which medical laboratories should comply in order to guarantee safety and effectiveness of medical test results. Although this is a global trend, medical laboratories across continents and countries are in distinct phases and experience various situations. Read More

    Orbitrap™ high-resolution mass spectrometry for the identification of amoxicillin crystalluria.
    Clin Chem Lab Med 2018 May 5. Epub 2018 May 5.
    Laboratory of Renal Lithiasis Research, University Institute of Health Sciences Research (IUNICS-IdISBa), University of Balearic Islands, Palma de Mallorca, Spain.

    Improving IBD diagnosis and monitoring by understanding preanalytical, analytical and biological fecal calprotectin variability.
    Clin Chem Lab Med 2018 May 5. Epub 2018 May 5.
    Department of Medicine - DIMED, University of Padova, Padova, Italy.
    Background: The appropriate clinical use of fecal calprotectin (fCal) might be compromised by incomplete harmonization between assays and within- and between-subjects variability. Our aim was to investigate the analytical and biological variability of fCal in order to provide tools for interpreting fCal in the clinical setting.

    Methods: Experiments were conducted to investigate the effects of temperature and storage time on fCal. Read More

    Improved prospective risk analysis for clinical laboratories compensated for the throughput in processes.
    Clin Chem Lab Med 2018 May 5. Epub 2018 May 5.
    Laboratory of Clinical Chemistry and Haematology/Semen Bank, Rijnstate Hospital, PO Box 9555, 6800 TA Arnhem, The Netherlands.
    Background: Practical application of prospective risk analysis (PRA) in clinical laboratories should reflect processes as they are carried out, while making the PRA results obtained from different processes comparable. This means that not only STAT and standard testing and testing for critical and less critical parameters should be distinguished (as published), but also that the throughput in processes and process steps should be taken into account.

    Methods: Building on our previously published PRA, a method was developed to compensate for the throughput in processes and process steps. Read More

    Verification of reference intervals in routine clinical laboratories: practical challenges and recommendations.
    Clin Chem Lab Med 2018 May 5. Epub 2018 May 5.
    Department of Pediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, ON, Canada.
    Reference intervals (RIs) are fundamental tools used by healthcare and laboratory professionals to interpret patient laboratory test results, ideally enabling differentiation of healthy and unhealthy individuals. Under optimal conditions, a laboratory should perform its own RI study to establish RIs specific for its method and local population. However, the process of developing RIs is often beyond the capabilities of an individual laboratory due to the complex, expensive and time-consuming process to develop them. Read More

    Harmonization in autoimmune thyroid disease diagnostics.
    Clin Chem Lab Med 2018 May 5. Epub 2018 May 5.
    Laboratory of Clinical Pathology, S. Maria degli Angeli Hospital, Pordenone, Italy.
    In this review we analyze all aspects of total testing process regarding the measurement of antithyroid peroxidase, antithyroglobulin and antithyrotropin receptor autoantibodies. The main critical points related to the preanalytical, analytical and postanalytical steps of autoimmune thyroid disease diagnostics are considered, focusing on harmonization of autoimmune thyroid tests request, retesting intervals, terminology of thyroid autoantibodies, measurement units and definition of reference limits. Harmonization in thyroid autoantibody testing is a relevant example of feasible harmonization in autoimmunology. Read More

    Laboratory sample stability. Is it possible to define a consensus stability function? An example of five blood magnitudes.
    Clin Chem Lab Med 2018 May 5. Epub 2018 May 5.
    Sociedad Española de Medicina de Laboratorio - Comisión de Calidad Extra analítica, Barcelona, Spain.
    Background: The stability limit of an analyte in a biological sample can be defined as the time required until a measured property acquires a bias higher than a defined specification. Many studies assessing stability and presenting recommendations of stability limits are available, but differences among them are frequent. The aim of this study was to classify and to grade a set of bibliographic studies on the stability of five common blood measurands and subsequently generate a consensus stability function. Read More

    Toward harmonization of clinical molecular diagnostic reports: findings of an international survey.
    Clin Chem Lab Med 2018 May 5. Epub 2018 May 5.
    The Chinese University of Hong Kong, Department of Chemical Pathology, Hong Kong SAR, P.R. China.
    Background: The International Organization for Standardization (ISO) 15189 standard provides recommendations for the postexamination reporting phase to enhance quality in clinical laboratories. The purpose of this study was to encourage a broad discussion on current reporting practices for molecular diagnostic tests by conducting a global survey of such practices.

    Methods: The International Federation of Clinical Chemistry and Laboratory Medicine's Committee for Molecular Diagnostics (IFCC C-MD) surveyed laboratories on selected ISO 15189 recommendations and topics. Read More

    Quantification of human complement C2 protein using an automated turbidimetric immunoassay.
    Clin Chem Lab Med 2018 May 5. Epub 2018 May 5.
    The Binding Site Group Limited, Birmingham, UK.
    Background: The measurement of complement components is clinically useful where a deficiency is suspected, or where excessive activation and consumption are present in disease. C2 deficiency carries an increased risk of developing systemic lupus erythematosus, recurrent infections and atherosclerosis. In this study, we have evaluated The Binding Site's Human Complement C2 SPAPLUS® assay. Read More

    Establishing reference intervals for sex hormones and SHBG in apparently healthy Chinese adult men based on a multicenter study.
    Clin Chem Lab Med 2018 May 5. Epub 2018 May 5.
    Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, P.R. China.
    Background: Measuring sex hormones is essential in diagnosing health issues such as testicular dysfunction, male infertility and feminization syndrome. However, there are no reports on reference intervals (RIs) in Chinese men. We conducted a nationwide multicenter study to establish RIs for seven sex hormones (luteinizing hormone [LH], follicle-stimulating hormone [FSH], prolactin [PRL], total testosterone [TT], free testosterone [FT], bioavailable testosterone [BAT] and estrogen [E2]), as well as sex hormone-binding globulin (SHBG). Read More

    Recent initiatives in harmonization of hemostasis practice.
    Clin Chem Lab Med 2018 May 3. Epub 2018 May 3.
    Section of Clinical Biochemistry, Department of Neurological, Biomedical and Movement Sciences, University of Verona, Verona, Italy.
    Accepting that standardizing and harmonizing laboratory practice has considerable value within laboratory medicine, including within the field of hemostasis, this paper concentrates on some recent and important initiatives in harmonizing hemostasis practice. Harmonization of hemostasis practice to improve clinical diagnosis and management is best driven by evidence and, in the absence of evidence, by consensus and expert opinion. To such end, there are various groups involved in such initiatives, and recent initiatives by these groups are highlighted in this review. Read More

    Harmonization of External Quality Assessment Schemes and their role - clinical chemistry and beyond.
    Clin Chem Lab Med 2018 May 1. Epub 2018 May 1.
    Department of Clinical Chemistry and Laboratory Medicine, Leiden University Medical Center, Leiden, The Netherlands.
    The commentary tries to reply to the following three questions: Are External Quality Assessment Schemes (EQAS) really fit for purpose? Are all schemes equivalent and sufficiently harmonized? Is the role of EQAS similar and necessary in all branches of laboratory medicine? Although the reply to the first two questions is, unfortunately, negative for several reasons (lack of commutable material with reference method values, EQAS with different scopes, etc.), the reply to the third one is positive: EQAS are a necessary source of information on trueness and accuracy and must be fully developed for all the branches of the clinical laboratory. Read More

    National surveys on internal quality control for blood gas analysis and related electrolytes in clinical laboratories of China.
    Clin Chem Lab Med 2018 May 1. Epub 2018 May 1.
    Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, P.R.China.
    Background: Internal quality control (IQC) is essential for precision evaluation and continuous quality improvement. This study aims to investigate the IQC status of blood gas analysis (BGA) in clinical laboratories of China from 2014 to 2017.

    Methods: IQC information on BGA (including pH, pCO2, pO2, Na+, K+, Ca2+, Cl-) was submitted by external quality assessment (EQA) participant laboratories and collected through Clinet-EQA reporting system in March from 2014 to 2017. Read More

    Updated review of postmortem biochemical exploration of hypothermia with a presentation of standard strategy of sampling and analyses.
    Clin Chem Lab Med 2018 May 1. Epub 2018 May 1.
    CURML, Centre Universitaire Romand de Médecine Légale, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
    Hypothermia is defined as a core body temperature below 35°C and can be caused by environmental exposure, drug intoxication, metabolic or nervous system dysfunction. This lethal pathology with medico-legal implications is complex to diagnose because macroscopic and microscopic lesions observed at the autopsy and the histological analysis are suggestive but not pathognomonic. Postmortem biochemical explorations have been progressively developed through the study of several biomarkers to improve the diagnosis decision cluster. Read More

    Procalcitonin guidance in patients with lower respiratory tract infections: a systematic review and meta-analysis.
    Clin Chem Lab Med 2018 May 1. Epub 2018 May 1.
    Faculty of Medicine, University of Basel, Basel, Switzerland.
    Although effective for bacterial lower respiratory tract infections (LRTIs), antibiotic treatment is often incorrectly prescribed for non-bacterial LRTIs. Procalcitonin has emerged as a promising biomarker to diagnose bacterial infections and guide antibiotic treatment decisions. As part of a regulatory submission to the U. Read More

    Prognostic implications of detectable cardiac troponin I below the 99th percentile in patients admitted to an emergency department without acute coronary syndrome.
    Clin Chem Lab Med 2018 May 1. Epub 2018 May 1.
    Cardiology Department, University Hospital Germans Trias Pujol, Badalona, Spain.
    Background: Detectable troponin below the 99th percentile may reflect an underlying cardiac abnormality which might entail prognostic consequences. This study aimed to investigate the prognosis of patients admitted to an emergency department (ED) with detectable troponin below the 99th percentile reference limit who did not present with an acute coronary syndrome (ACS).

    Methods: We analysed the clinical data of all consecutive patients admitted to the ED during the years 2012 and 2013 in whom cardiac troponin was requested by the attending clinician (cTnI Ultra Siemens, Advia Centaur). Read More

    Computer-assisted interventions in the clinical laboratory process improve the diagnosis and treatment of severe vitamin B12 deficiency.
    Clin Chem Lab Med 2018 May 1. Epub 2018 May 1.
    Department of Radiology, University of Missouri, Columbia, MO, USA.
    Background: Severe vitamin B12 deficiency can result in serious complications if undiagnosed or untreated. Our aim was to test the efficacy of interventions in the laboratory process to improve the detection and the treatment of severe vitamin B12 deficiency.

    Methods: Quasi-experimental investigation with a retrospective 7-year pre-intervention period and 29-month post-intervention follow-up in a university hospital. Read More

    A quantitative LC-MS/MS method for insulin-like growth factor 1 in human plasma.
    Clin Chem Lab Med 2018 May 1. Epub 2018 May 1.
    Bioanalytical Laboratory, PRA Health Sciences, Amerikaweg 18, 9407 TK Assen, The Netherlands.
    Background: Insulin-like growth factor 1 (IGF1) is a biomarker with various applications in medicine and also in doping control.

    Methods: A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed that employs 15N-IGF1 as an internal standard. The method features urea-based IGF1/IGFBP-complex dissociation which is directly followed by tryptic digestion. Read More

    Diagnostic laboratory tests for systemic autoimmune rheumatic diseases: unmet needs towards harmonization.
    Clin Chem Lab Med 2018 May 1. Epub 2018 May 1.
    Experimental Laboratory of Immunological and Rheumatologic Researches, IRCCS Istituto Auxologico Italiano, Via Zucchi 18, 20095 Cusano Milanino, Milan, Italy.
    Autoantibodies are helpful tools not only for the diagnosis and the classification of systemic autoimmune rheumatic diseases (SARD) but also for sub-grouping patients and/or for monitoring disease activity or specific tissue/organ damage. Consequently, the role of the diagnostic laboratory in the management of SARD is becoming more and more important. The advent of new techniques raised the need of updating and harmonizing our use/interpretation of the assays. Read More

    Fibroblast growth factor 23 and renal function among young and healthy individuals.
    Clin Chem Lab Med 2018 May 1. Epub 2018 May 1.
    Labormedizinisches Zentrum Dr. Risch, Vaduz, Principality ofLiechtenstein.
    Background: Fibroblast growth factor 23 (FGF-23), an osteocyte hormone involved in the regulation of phosphate metabolism, is associated with incident and progressive chronic kidney disease. We aimed to assess the association of FGF-23 with renal parameters, vascular function and phosphate metabolism in a large cohort of young and healthy individuals.

    Methods: Healthy individuals aged 25-41 years were included in a prospective population-based study. Read More

    External quality assessment schemes for glucose measurements in Germany: factors for successful participation, analytical performance and medical impact.
    Clin Chem Lab Med 2018 May 1. Epub 2018 May 1.
    Institut für Klinische Chemie und Pathobiochemie, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany.
    Background: Determination of blood glucose concentration is one of the most important measurements in clinical chemistry worldwide. Analyzers in central laboratories (CL) and point-of-care tests (POCT) are both frequently used. In Germany, regular participation in external quality assessment (EQA) schemes is mandatory for laboratories performing glucose testing. Read More

    Quantification of vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid in urine using a dilute-and-shoot and ultra-high pressure liquid chromatography tandem mass spectrometry method.
    Clin Chem Lab Med 2018 May 1. Epub 2018 May 1.
    Laboratoire des Catecholamines et Peptides, Service de Pharmacologie Clinique, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
    Background: Urinary vanillylmandelic acid (VMA) and homovanillic acid (HVA) are biomarkers for the diagnosis and follow-up of neuroblastoma, whereas urinary 5-hydroxyindoleacetic acid (5-HIAA) is used to assess a carcinoid tumor. These analytes are conventionally analyzed in a single run by chromatography (LC) coupled with electrochemical detection (LC-ECD) using commercial kits. A rapid dilute-and-shoot LC tandem mass spectrometry (LC-MS/MS) assay was validated in order to replace the LC-ECD method and therefore improve analytical specificity and throughput. Read More

    Detecting molecular forms of antithrombin by LC-MRM-MS: defining the measurands.
    Clin Chem Lab Med 2018 May 1. Epub 2018 May 1.
    Department of Clinical Chemistry and Laboratory Medicine, Leiden University Medical Center, Leiden, TheNetherlands.
    Background: Antithrombin (AT) is a critical regulator of coagulation, and its overall activity is typically measured using functional tests. A large number of molecular forms of AT have been identified and each individual carries multiple molecular proteoforms representing variable activities. Conventional functional tests are completely blind for these proteoforms. Read More

    HCV core antigen comes of age: a new opportunity for the diagnosis of hepatitis C virus infection.
    Clin Chem Lab Med 2018 May;56(6):880-888
    Department of Laboratory Medicine, University-Hospital of Padova, Padova, Italy.
    The diagnosis of hepatitis C virus (HCV) infection has been traditionally based on the detection of the host antibody response. Although antibody assays are available in different formats and are fairly accurate, they cannot distinguish between an ongoing infection with HCV replicative activity and a past infection where HCV has been cleared, spontaneously or after a successful therapy. As a chronic infection is mostly asymptomatic until the late clinical stages, there is a compelling need to detect active HCV infection by simple and reproducible methods. Read More

    Measurement uncertainty and metrological traceability of whole blood cyclosporin A mass concentration results obtained by UHPLC-MS/MS.
    Clin Chem Lab Med 2018 Apr 24. Epub 2018 Apr 24.
    Laboratori de Referència d'Enzimologia Clínica, Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain.
    Background: Traceable and accurate results of cyclosporine A (CsA) mass concentrations in whole blood are required to ensure the monitoring of immunosuppressive therapy in transplant recipients. Metrological traceability and measurement uncertainty can allow ensuring reliability and comparability of these results over time and space. In this study, we provide a practical and detailed example of how the traceability and uncertainty of mass concentration of CsA results, obtained using an ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) procedure, can be described and estimated. Read More

    High microRNA-28-5p expression in colorectal adenocarcinoma predicts short-term relapse of node-negative patients and poor overall survival of patients with non-metastatic disease.
    Clin Chem Lab Med 2018 May;56(6):990-1000
    Department of Biochemistry and Molecular Biology, National and Kapodistrian University of Athens, Athens, Greece, Phone: +30 2107274306, Fax: +30 2107274158.
    Background: MicroRNAs (miRNAs) may function either as oncogenes or tumor suppressors and are heavily involved in the initiation and progression of cancer, and in metastasis of tumor cells. MicroRNA-28-5p (miR-28-5p) targets several cancer-related genes and is hence involved in cell proliferation, migration, invasion and epithelial-mesenchymal transition. In this study, we investigated the potential diagnostic and prognostic significance of miR-28-5p expression in colorectal adenocarcinoma, the most frequent type of colorectal cancer (CRC). Read More

    Uncertainty evaluation in clinical chemistry, immunoassay, hematology and coagulation analytes using only external quality assessment data.
    Clin Chem Lab Med 2018 Apr 23. Epub 2018 Apr 23.
    Department of Clinical Laboratory, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, P.R. China.
    Background: Measurement uncertainty (MU) is a parameter associated with the result of a measurement that characterizes its dispersion. We report results for estimating MU following the application of a top-down procedure using only proficiency test data to establish uncertainty levels for various analytes.

    Methods: Data were obtained from 142 laboratories participating in the Beijing Center for Clinical Laboratory (BCCL) proficiency testing/external quality assessment (PT/EQA) schemes. Read More

    1 OF 117