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    5638 results match your criteria Clinical Chemistry and Laboratory Medicine CCLM [Journal]

    1 OF 113

    Is irisin the new player in exercise-induced adaptations or not? A 2017 update.
    Clin Chem Lab Med 2017 Nov 11. Epub 2017 Nov 11.
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    Irisin is produced by a proteolytic cleavage of fibronectin type III domain-containing protein 5 (FNDC5) and has emerged as a potential mediator of exercise-induced energy metabolism. The purpose of this study was to review the results of studies that investigated irisin responses to acute and chronic exercise and provide an update. A comprehensive search in the databases of MEDLINE was performed (74 exercise studies). Read More

    Harmonization in laboratory medicine: more than clinical chemistry?
    Clin Chem Lab Med 2017 Nov 2. Epub 2017 Nov 2.
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    The goal of harmonizing laboratory information is to contribute to quality in patient care, ultimately improving upon patient outcomes and safety. The main focus of harmonization and standardization initiatives has been on analytical processes within the laboratory walls, clinical chemistry tests in particular. However, two major evidences obtained in recent years show that harmonization should be promoted not only in the analytical phase but also in all steps of the testing process, encompassing the entire field of laboratory medicine, including innovative areas (e. Read More

    Visual assessment of hemolysis affects patient safety.
    Clin Chem Lab Med 2017 Nov 2. Epub 2017 Nov 2.
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    Background: Manual handling of hemolyzed samples is not standardized and is vulnerable to errors. This study aimed to evaluate laboratory errors due to manual handling of hemolyzed samples and to assess the risk they might have for patient safety.

    Methods: Data were retrospectively obtained from a laboratory information system for 25 emergency tests from hemolyzed samples. Read More

    Reference intervals for stone risk factors in 24-h urine among healthy adults of the Han population in China.
    Clin Chem Lab Med 2017 Nov 2. Epub 2017 Nov 2.
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    Background: The aim of the study was to establish reference intervals for 24-h urinary stone risk factors in the healthy Chinese Han population.

    Methods: From May 2013 to July 2014, we collected and analyzed 24-h urine samples from healthy adult Han population during a cross-sectional study across China. The protocol for analysis of 24-h urine included volume, pH, oxalate, citrate, sodium, potassium, chloride, calcium, phosphorous, creatinine, urate, magnesium, the ion activity products of calcium oxalate (AP(CaOx) indexs) and calcium phosphate (AP(CaP) indexs). Read More


    Application of κ free light chains in cerebrospinal fluid as a biomarker in multiple sclerosis diagnosis: development of a diagnosis algorithm.
    Clin Chem Lab Med 2017 Oct 31. Epub 2017 Oct 31.
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    Background: The determination of κ free light chains (KFLC) in cerebrospinal fluid (CSF) by nephelometry is a feasible alternative to immunoglobulin G oligoclonal bands (OCB) in the evaluation of intrathecal synthesis of immunoglobulin in multiple sclerosis (MS) and other demyelinating diseases. The aim of this study was to assess the diagnostic value of KFLC and its inclusion in a procedure algorithm along with OCB interpretation.

    Methods: A cross-sectional study, which included 123 patients with a CSF OCB request, was carried out. Read More

    Reaching consensus on communication of critical laboratory results using a collective intelligence method.
    Clin Chem Lab Med 2017 Oct 23. Epub 2017 Oct 23.
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    Background: There is no consensus in the literature about what analytes or values should be informed as critical results and how they should be communicated. The main aim of this project is to establish consensual standards of critical results for the laboratories participating in the study. Among the project's secondary objectives, establishing consensual procedures for communication can be highlighted. Read More

    Machine learning in laboratory medicine: waiting for the flood?
    Clin Chem Lab Med 2017 Oct 23. Epub 2017 Oct 23.
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    This review focuses on machine learning and on how methods and models combining data analytics and artificial intelligence have been applied to laboratory medicine so far. Although still in its infancy, the potential for applying machine learning to laboratory data for both diagnostic and prognostic purposes deserves more attention by the readership of this journal, as well as by physician-scientists who will want to take advantage of this new computer-based support in pathology and laboratory medicine. Read More

    Strong impact on plasma protein profiles by precentrifugation delay but not by repeated freeze-thaw cycles, as analyzed using multiplex proximity extension assays.
    Clin Chem Lab Med 2017 Oct 17. Epub 2017 Oct 17.
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    Background A number of factors regarding blood collection, handling and storage may affect sample quality. The purpose of this study was to assess the impact on plasma protein profiles by delayed centrifugation and plasma separation and multiple freeze-thaw cycles. Methods Blood samples drawn from 16 healthy individuals were collected into ethylenediaminetetraacetic acid tubes and kept either at 4 °C or 22 °C for 1-36 h prior to centrifugation. Read More

    Validation of the Six Sigma Z-score for the quality assessment of clinical laboratory timeliness.
    Clin Chem Lab Med 2017 Oct 17. Epub 2017 Oct 17.
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    Background: The International Federation of Clinical Chemistry and Laboratory Medicine has introduced in recent times the turnaround time (TAT) as mandatory quality indicator for the postanalytical phase. Classic TAT indicators, namely, average, median, 90th percentile and proportion of acceptable test (PAT), are in use since almost 40 years and to date represent the mainstay for gauging the laboratory timeliness. In this study, we investigated the performance of the Six Sigma Z-score, which was previously introduced as a device for the quantitative assessment of timeliness. Read More

    Strategies to define performance specifications in laboratory medicine: 3 years on from the Milan Strategic Conference.
    Clin Chem Lab Med 2017 Oct;55(12):1849-1856
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    Measurements in clinical laboratories produce results needed in the diagnosis and monitoring of patients. These results are always characterized by some uncertainty. What quality is needed and what measurement errors can be tolerated without jeopardizing patient safety should therefore be defined and specified for each analyte having clinical use. Read More

    How to reduce scientific irreproducibility: the 5-year reflection.
    Clin Chem Lab Med 2017 Oct;55(12):1845-1848
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    We discuss in depth six causes of scientific irreproducibility and their ramifications for the clinical sciences: fraud, unfounded papers published by prominent authorities, bias, technical deficiencies, fragmented science and problems with big data. Some proposed methods to combat this problem are briefly described, including an effort to replicate results from some high impact papers and a proposal that authors include detailed preclinical data in papers with supposedly high translational value. We here advocate for a 5-year reflection on papers with seemingly high clinical/translational potential, published alongside the original paper where authors reflect on the quality, reproducibility and impact of their findings. Read More

    The venous thromboembolic risk and the clot wave analysis: a useful relationship?
    Clin Chem Lab Med 2017 Oct 14. Epub 2017 Oct 14.
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    Background: Hospitalized patients with acute medical conditions have higher venous thromboembolism (VTE) risk. A patient with a final Padua Prediction Score (PPS) of ≥4 is considered to be at high risk for VTE. The aim of this study was to investigate on a possible relationship between PPS, the dynamics of the clot formation, i. Read More

    Indirect method for validating transference of reference intervals.
    Clin Chem Lab Med 2017 Oct 14. Epub 2017 Oct 14.
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    Background: Transference of reference intervals (RIs) from multicentre studies are often verified by use of a small number of samples from reference individuals or by the use of one serum sample (Serum X for NORIP RI). Despite recommended and appropriate methods, both have inconveniencies and drawbacks. Several attempts have been made to develop an indirect method, which uses historical data from the laboratory. Read More

    Intragenic hypomethylation of DNMT3A in patients with myelodysplastic syndrome.
    Clin Chem Lab Med 2017 Oct 14. Epub 2017 Oct 14.
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    Background: DNMT3A is a DNA methyltransferase that acts in de novo methylation. Aberrant expression of DNMT3A has been reported in several human diseases, including myelodysplastic syndrome (MDS). However, the pattern of DNMT3A methylation remains unknown in MDS. Read More

    Laboratory performance of sweat conductivity for the screening of cystic fibrosis.
    Clin Chem Lab Med 2017 Oct 9. Epub 2017 Oct 9.
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    Background: There are several complementary English-language guidelines for the performance of the sweat chloride test. These guidelines also incorporate information for the collection of conductivity samples. However, recommendations for the measurement and reporting of sweat conductivity are less clear than for sweat chloride. Read More

    Differences in levels of albumin, ALT, AST, γ-GT and creatinine in frail, moderately healthy and healthy elderly individuals.
    Clin Chem Lab Med 2017 Oct 9. Epub 2017 Oct 9.
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    Background: Reference intervals are widely used as decision tools, providing the physician with information about whether the analyte values indicate ongoing disease process. Reference intervals are generally based on individuals without diagnosed diseases or use of medication, which often excludes elderly. The aim of the study was to assess levels of albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine and γ-glutamyl transferase (γ-GT) in frail, moderately healthy and healthy elderly indivuduals. Read More

    GFR estimation based on standardized creatinine and cystatin C: a European multicenter analysis in older adults.
    Clin Chem Lab Med 2017 Oct 6. Epub 2017 Oct 6.
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    Background: Although recommended by the Kidney Disease Improving Global Outcomes, the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPICR) creatinine equation was not targeted to estimate glomerular filtration rate (eGFR) among older adults. The Berlin Initiative Study (BIS1CR) equation was specifically developed in older adults, and the Lund-Malmö revised (LMRCR) and the Full Age Spectrum (FASCR) equations have shown promising results in older adults. Our aim was to validate these four creatinine equations, including addition of cystatin C in a large multicenter cohort of Europeans ≥70 years. Read More

    Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients.
    Clin Chem Lab Med 2017 Oct 6. Epub 2017 Oct 6.
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    Background: Colorimetric albumin assays based on binding to bromocresol purple (BCP) and bromocresol green (BCG) yield different results in chronic kidney disease. Altered dye binding of carbamylated albumin has been suggested as a cause. In the present study, a detailed analysis was carried out in which uremic toxins, acute phase proteins and Kt/V, a parameter describing hemodialysis efficiency, were compared with colorimetrically assayed (BCP and BCG) serum albumin. Read More

    Interlaboratory variability of urinary iodine measurements.
    Clin Chem Lab Med 2017 Sep 23. Epub 2017 Sep 23.
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    Background: The iodine status of populations is usually assessed by median urinary iodine concentrations (UIC) in population-based studies, but it is unclear to which extent UIC are comparable across different laboratories. The aim of our study was to investigate the variability of UIC measurements across three well-established German laboratories with long-term clinical-chemical expertise in iodine measurements and to compare these results to the gold standard inductively coupled plasma mass spectrometry (ICP-MS).

    Methods: UIC levels were measured from 303 urine samples derived from the "Dortmund Nutritional and Anthropometric Longitudinally Designed Study" and from volunteers of the University Medicine Greifswald at four different German laboratories. Read More

    Autovalidation and automation of the postanalytical phase of routine hematology and coagulation analyses in a university hospital laboratory.
    Clin Chem Lab Med 2017 Sep 23. Epub 2017 Sep 23.
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    Background: The need to satisfy high-throughput demands for laboratory tests continues to be a challenge. Therefore, we aimed to automate postanalytical phase in hematology and coagulation laboratory by autovalidation of complete blood count (CBC) and routine coagulation test results (prothrombin time [PT], international normalized ratio [PT-INR], activated partial thromboplastin time [APTT], fibrinogen, antithrombin activity [AT] and thrombin time [TT]). Work efficacy and turnaround time (TAT) before and after implementation of automated solutions will be compared. Read More

    Evaluation of analytical performance of a new high-sensitivity immunoassay for cardiac troponin I.
    Clin Chem Lab Med 2017 Sep 23. Epub 2017 Sep 23.
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    Background: The study aim was to evaluate and compare the analytical performance of the new chemiluminescent immunoassay for cardiac troponin I (cTnI), called Access hs-TnI using DxI platform, with those of Access AccuTnI+3 method, and high-sensitivity (hs) cTnI method for ARCHITECT platform.

    Methods: The limits of blank (LoB), detection (LoD) and quantitation (LoQ) at 10% and 20% CV were evaluated according to international standardized protocols. For the evaluation of analytical performance and comparison of cTnI results, both heparinized plasma samples, collected from healthy subjects and patients with cardiac diseases, and quality control samples distributed in external quality assessment programs were used. Read More

    Insulin autoimmune syndrome (Hirata's disease) in an Italian patient: a case report and review of the literature.
    Clin Chem Lab Med 2017 Sep 16. Epub 2017 Sep 16.
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    We describe the case of a 54-year-old Caucasian Italian male experiencing episodes of hypoglycemia, occurring mainly after meals. He had never been exposed to insulin and was taking ramipril, flecainide and acetylsalicylic acid. An oral glucose tolerance test (OGTT) showed high blood glucose levels diagnostic for diabetes mellitus at 120 min and hypoglycemia with inappropriately high insulin levels at 240 min. Read More

    Making sense of a haemolysis monitoring and reporting system: a nationwide longitudinal multimethod study of 68 Australian laboratory participant organisations.
    Clin Chem Lab Med 2017 Sep 15. Epub 2017 Sep 15.
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    Background: The key incident monitoring and management systems (KIMMS) quality assurance program monitors incidents in the pre- and postanalytical phases of testing in medical laboratories. Haemolysed specimens have been found to be the most frequent preanalytical error and have major implications for patient care. The aims of this study were to assess the suitability of KIMMS for quality reporting of haemolysis and to devise a meaningful method for reporting and monitoring haemolysis. Read More

    MEF2C loss-of-function mutation associated with familial dilated cardiomyopathy.
    Clin Chem Lab Med 2017 Sep 13. Epub 2017 Sep 13.
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    Background: The MADS-box transcription factor myocyte enhancer factor 2C (MEF2C) is required for the cardiac development and postnatal adaptation and in mice-targeted disruption of the MEF2C gene results in dilated cardiomyopathy (DCM). However, in humans, the association of MEF2C variation with DCM remains to be investigated.

    Methods: The coding regions and splicing boundaries of the MEF2C gene were sequenced in 172 unrelated patients with idiopathic DCM. Read More

    Irregular analytical errors in diagnostic testing - a novel concept.
    Clin Chem Lab Med 2017 Sep 13. Epub 2017 Sep 13.
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    Background: In laboratory medicine, routine periodic analyses for internal and external quality control measurements interpreted by statistical methods are mandatory for batch clearance. Data analysis of these process-oriented measurements allows for insight into random analytical variation and systematic calibration bias over time. However, in such a setting, any individual sample is not under individual quality control. Read More

    Can a combination of average of normals and "real time" External Quality Assurance replace Internal Quality Control?
    Clin Chem Lab Med 2017 Sep 13. Epub 2017 Sep 13.
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    Internal Quality Control and External Quality Assurance are separate but related processes that have developed independently in laboratory medicine over many years. They have different sample frequencies, statistical interpretations and immediacy. Both processes have evolved absorbing new understandings of the concept of laboratory error, sample material matrix and assay capability. Read More

    Stability of routine biochemical analytes in whole blood and plasma/serum: focus on potassium stability from lithium heparin.
    Clin Chem Lab Med 2017 Sep 9. Epub 2017 Sep 9.
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    Background: Blood specimens are transported from clinical departments to the biochemistry laboratory by hospital courier service, sometimes over long distances. The aim of this study was to assess the stability of common biochemical analytes in venous blood under our routine transport conditions and to evaluate analyte stability after prompt or delayed centrifugation.

    Methods: We investigated pre- and postanalytical contributions of 32 biochemical analytes in plasma and serum samples from 10 patients (healthy adults and patients from intensive care units). Read More

    Comparative study of the diagnostic and prognostic value of antibodies against chimeric citrullinated synthetic peptides and CCP3/CCP3.1 assays.
    Clin Chem Lab Med 2017 Aug 29. Epub 2017 Aug 29.
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    Background: The objective of the study was to compare the diagnostic yield of home-made ELISA tests based on synthetic chimeric fibrin/filaggrin citrullinated peptides (CFFCPs) with CCP3 and CCP3.1 commercial tests to detect anti-citrullinated protein/peptide antibodies (ACPAs) in rheumatoid arthritis (RA) patients. The prognostic value is also studied in a cohort of patients with early RA. Read More

    The effects of dry ice exposure on plasma pH and coagulation analyses.
    Clin Chem Lab Med 2017 Aug 28. Epub 2017 Aug 28.
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    Background: We recently observed that exposure to dry ice lowered sample pH and increased clotting times in lupus anticoagulant analyses, and that such changes could be prevented by placing samples at -80°C after dry ice exposure. In the current study, we sought to evaluate the effects of dry ice exposure on pH and various commonly used coagulation analyses.

    Methods: Citrated plasma from 30 healthy blood donors was allocated to four preanalytical regimes: (1) immediate analysis of fresh plasma or (2) storage at -20°C; (3) storage at -20°C followed by dry ice exposure for 24 h or (4) storage at -20°C followed by dry ice exposure for 24 h and storage at -80°C for 24 h before analysis. Read More

    Update in diagnosis and management of primary aldosteronism.
    Clin Chem Lab Med 2017 Aug 28. Epub 2017 Aug 28.
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    Primary aldosteronism (PA) is a group of disorders in which aldosterone is excessively produced. These disorders can lead to hypertension, hypokalemia, hypervolemia and metabolic alkalosis. The prevalence of PA ranges from 5% to 12% around the globe, and the most common causes are adrenal adenoma and adrenal hyperplasia. Read More

    Use of the sFlt-1/PlGF ratio to rule out preeclampsia requiring delivery in women with suspected disease. Is the evidence reproducible?
    Clin Chem Lab Med 2017 Aug 25. Epub 2017 Aug 25.
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    Background: Soluble fms-like tyrosine kinase 1 (sFlt-1) to placental growth factor (PlGF) ratio has been proven to predict preeclampsia occurrence.

    Methods: Blood samples from 195 pregnant women with suspected preeclampsia were obtained at obstetric triage admission or from the high-risk pregnancy outpatient office. Serum PlGF and sFlt-1 were measured by an electrochemiluminescence immunoassay (ECLIA) on the immunoanalyser Cobas e601 (Roche Diagnostics) and the corresponding ratio was calculated. Read More

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