Measurement of Lipoprotein-Associated Phospholipase A2 by Use of 3 Different Methods: Exploration of Discordance between ELISA and Activity Assays.
- Celalettin Topbas,
- Alan Swick,
- Morteza Razavi,
- N Leigh Anderson,
- Terry W Pearson,
- Cory Bystrom
Clin Chem 2018 Jan 10. Epub 2018 Jan 10.
Cleveland HeartLab, Cleveland, OH;
Background: Lipoprotein-associated phospholipase A2 (Lp-PLA2), an enzyme associated with inflammation, is used as a biomarker for cardiovascular disease risk. Both the concentration and activity of Lp-PLA2 have been shown to be clinically relevant. However, there is a discordance between the serum concentration of Lp-PLA2 measured by the standard ELISA-based immunoassays and the activity of this enzyme, leading to substantial discordance in risk categorization depending on assay format. Read More