6,468 results match your criteria Clinical Biochemistry [Journal]


Laboratory intervention to improve the request of urinary albumin in primary care patients with arterial hypertension and financial implications.

Clin Biochem 2019 Apr 16. Epub 2019 Apr 16.

Department of Radiology, University of Missouri, Columbia, MO, USA.

Introduction: The request of Urinary albumin in primary care in Spain is insufficient to monitor patients with diabetes and hypertension (HTN). Our aim was to evaluate a strategy designed in consensus with general practitioners (GPs) to improve the request of urinary albumin in primary care patients with HTN according to guidelines, and to study its financial implications.

Materials And Methods: In a meeting with GPs, we decided that the Laboratory Information System (LIS) would automatically register the albumin-to-creatinine ratio (ACR) test in patients with HTN when the former had not been requested in the previous year. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120193006
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http://dx.doi.org/10.1016/j.clinbiochem.2019.04.012DOI Listing
April 2019
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Differential diagnosis between Alzheimer's disease and other dementias: Role of cerebrospinal fluid biomarkers.

Clin Biochem 2019 Apr 15. Epub 2019 Apr 15.

Centre for Memory Disturbances, Laboratory of Clinical Neurochemistry, Section of Neurology, Department of Medicine, University of Perugia, Perugia, Italy. Electronic address:

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http://dx.doi.org/10.1016/j.clinbiochem.2019.04.011DOI Listing

Identification of novel and rare CYP21A2 variants in Chinese patients with congenital adrenal hyperplasia due to 21-hydroxylase deficiency.

Authors:
Jing Xu Pin Li

Clin Biochem 2019 Apr 14. Epub 2019 Apr 14.

Department of Endocrinology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200062, China. Electronic address:

Objective: 21-hydroxylase deficiency (21-OHD) is the most common cause of congenital adrenal hyperplasia due to CYP21A2 gene mutation. The aim of study is to expand CYP21A2 mutational spectrum in the Chinese population and to provide novel genetic information in terms of ethnic diversity.

Design And Methods: 95 Chinese suspected 21-OHD patients with phenotypes varying from salt-wasting (SW) to nonclassic symptoms were recruited. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183137
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http://dx.doi.org/10.1016/j.clinbiochem.2019.04.008DOI Listing
April 2019
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Towards salivary C-reactive protein as a viable biomarker of systemic inflammation.

Clin Biochem 2019 Apr 14. Epub 2019 Apr 14.

Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4RN, UK. Electronic address:

C-reactive protein (CRP) is a commonly used marker of systemic inflammation, routinely measured in serum blood samples. However salivary samples offer a non-invasive and easily accessible alternative which would improve point of care (POC) testing for inflammation. Two major challenges restrict the use of saliva: the influence of the oral environment on CRP and its local production; and collecting a standardised sample given patient-dependent salivary flow rates. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183091
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http://dx.doi.org/10.1016/j.clinbiochem.2019.04.006DOI Listing
April 2019
2 Reads

Commonly used fluoroquinolones cross-react with urine drug screens for opiates, buprenorphine, and amphetamines.

Clin Biochem 2019 Apr 13. Epub 2019 Apr 13.

Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, 1301 Medical Center Drive, Nashville, TN 37232, United States.

Objectives: Fluoroquinolone antibiotics are commonly used in the treatment of infections and have previously been confirmed to cross-react with previous generations of opiates immunoassays. In this work we evaluated the cross-reactivity of the three fluoroquinolones in use at our institution with a panel of 10 urine drug screens.

Design And Methods: Drug preparations of levofloxacin, ciprofloxacin, and moxifloxacin that were designed for intravenous delivery were added to drug-free urine at varying concentrations. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120193009
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http://dx.doi.org/10.1016/j.clinbiochem.2019.04.009DOI Listing
April 2019
3 Reads

Differential expression of miR-155 and Let-7a in the plasma of childhood asthma: Potential biomarkers for diagnosis and severity.

Clin Biochem 2019 Apr 11. Epub 2019 Apr 11.

Department of Pediatric, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

Background: MicroRNAs (miRNAs) are emerging gene expression regulators and their expression has been linked to various biological processes. However, the role of miRNAs in the regulation of allergic inflammatory disease still not clearly understood.

Aim: Our study was designed to investigate circulating miR-155 and Let-7a expression levels in the plasma of asthmatic children and healthy controls. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.04.007DOI Listing

Severe hot flashes and hospitalization after supposed niacin flushing in a woman undergoing pre-employment urine drug testing.

Clin Biochem 2019 Apr 8. Epub 2019 Apr 8.

Department of Pathology & Laboratory Medicine, PennState University, Hershey Medical Center, Hershey, PA, USA.

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http://dx.doi.org/10.1016/j.clinbiochem.2019.04.005DOI Listing

Blood-based molecular signature of Alzheimer's disease via spectroscopy and metabolomics.

Clin Biochem 2019 Apr 4. Epub 2019 Apr 4.

Department of Analytical Chemistry, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic.

Objectives: With over 35 million cases worldwide, Alzheimer's disease (AD) represents the main cause of dementia. The differentiation of AD from other types of dementia is challenging and its early diagnosis is complicated. The established biomarkers are not only based on the invasive collection of cerebrospinal fluid, but also lack sufficient sensitivity and specificity. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.04.004DOI Listing
April 2019
1 Read

Neuro-inflammation and anti-inflammatory treatment options for Alzheimer's disease.

Clin Biochem 2019 Apr 4. Epub 2019 Apr 4.

Department of Neurology, Antalya Training and Research Hospital, Antalya, Turkey.

Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive memory loss and dementia. The pathological characteristics of AD include the deposition of amyloid beta (Aβ), neurofibrillary tangles, and neuronal loss. There is evidence showing the involvement of inflammation in AD, including activated microglia within and surrounding senile plaques. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.04.001DOI Listing
April 2019
2 Reads

Plasma amyloid beta levels and platelet mitochondrial respiration in patients with Alzheimer's disease.

Clin Biochem 2019 Apr 4. Epub 2019 Apr 4.

Department of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic. Electronic address:

Objectives: Altered amyloid metabolism and mitochondrial dysfunction play key roles in the development of Alzheimer's disease (AD). We asked whether an association exists between disturbed platelet mitochondrial respiration and the plasma concentrations of Aβ and Aβ in patients with AD.

Design And Methods: Plasma Aβ and Aβ concentrations and mitochondrial respiration in intact and permeabilized platelets were measured in 50 patients with AD, 15 patients with vascular dementia and 25 control subjects. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.04.003DOI Listing

Search for biomarkers of Alzheimer's disease: Recent insights, current challenges and future prospects.

Clin Biochem 2019 Apr 4. Epub 2019 Apr 4.

Department of Analytical Chemistry, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic. Electronic address:

Due to the trend of prolonged lifespan leading to higher incidence of age-related diseases, the demand for reliable biomarkers of dementia rises. In this review, we present novel biomarkers of high potential, especially those found in blood, urine or saliva, which could lead to a more comfortable patient experience and better time- and cost-effectivity, compared to the currently used diagnostic methods. We focus on biomarkers that might allow for the detection of Alzheimer's disease before its clinical manifestations. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.04.002DOI Listing
April 2019
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Prognostic significance of miR-34 rs4938723 T > C polymorphism in triple negative breast cancer patients.

Clin Biochem 2019 Mar 29. Epub 2019 Mar 29.

1(st) Propaedeutic Surgical, Department, Hippocrateio Hospital, University of Athens, Greece.

Objectives: The aim of this was the assessment of the prognostic role of the rs4938723 C > T polymorphism of the miR-34 in triple negative breast cancer patients.

Methods: Therefore formalin fixed paraffin embedded tissue samples from 114 triple negative breast cancer patients and blood samples from 124 healthy donors were genotyped and subsequently extensive statistical analysis was performed in order to investigate the clinical value of this polymorphism in triple negative breast cancer.

Results: Our statistical analysis disclosed that the majority of patients harboring ductal breast carcinoma (69. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.009DOI Listing
March 2019
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Biomarker panel in sleep apnea patients after an acute coronary event.

Clin Biochem 2019 Mar 28. Epub 2019 Mar 28.

Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Madrid, Spain; Respiratory Department, Group of Translational Research in Respiratory Medicine, Hospital Universitari Arnau de Vilanova and Santa Maria, IRB Lleida, Lleida, Catalonia, Spain.

Background: Acute coronary syndrome (ACS) is a major cause of death and closely related with obstructive sleep apnea (OSA). Our hypothesis is that several cardiovascular-related biomarkers could have a differential prognostic value for ACS severity in patients with OSA, and could also help (individually or combined) in the detection of OSA in patients after a coronary event.

Methods: Up to 361 consecutive individuals admitted due to ACS were included in the study. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.015DOI Listing
March 2019
2 Reads

Impact of testosterone assay standardization efforts assessed via accuracy-based proficiency testing.

Clin Biochem 2019 Mar 28. Epub 2019 Mar 28.

Centers for Disease Control and Prevention, Atlanta, GA, United States of America. Electronic address:

Background: We reported observations on analytical performance in testosterone measurements of various methods/assays from the study carried out using accuracy-based proficiency testing (PT) during 2012-2013. In 2016, we re-evaluated analytical performance of testosterone assays using accuracy-based PT to assess effectiveness of CDC efforts toward standardization.

Methods: Five single-donor human serum samples from female and male adult donors were analyzed for testosterone by New York State Department of Health-certified clinical laboratories using 16 immunoassays and LC-MS/MS methods. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.014DOI Listing
March 2019
2 Reads

Erratum to 'Editorial: Biomarkers in reproductive health' [Clinical Biochemistry 62 (2018) 1].

Clin Biochem 2019 Apr 28;66:108. Epub 2019 Mar 28.

Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, Avenue Mounier 52, 1200 Brussels, Belgium; Department of Clinical Biochemistry, Cliniques Universitaires Saint-Luc, Avenue Hippocrate 10, 1200 Brussels, Belgium. Electronic address:

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.011DOI Listing

Erratum to 'Oxytocin testing and reproductive health: Status and clinical applications' [Clinical Biochemistry 62 (2018) 55-61].

Authors:
Damien Gruson

Clin Biochem 2019 Apr 26;66:107. Epub 2019 Mar 26.

Department of Laboratory Medicine, Cliniques Universitaires St-Luc, Université Catholique de Louvain, Brussels, Belgium; Pôle de recherche en Endocrinologie, Diabète et Nutrition, Institut de Recherche Expérimentale et Clinique, Cliniques Universitaires St-Luc, Université Catholique de Louvain, Brussels, Belgium. Electronic address:

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.010DOI Listing

Development and validation of a LC-MS/MS method for the establishment of reference intervals and biological variation for five plasma steroid hormones.

Clin Biochem 2019 Mar 25. Epub 2019 Mar 25.

Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. Electronic address:

Background: With liquid chromatography-tandem mass spectrometry (LC-MS/MS) increasingly being used for the quantification of steroid hormones, there is a need for studies that re-establish reference intervals and biological variation in well-defined cohorts.

Methods: A plasma steroid hormone profiling method using LC-MS/MS for quantification of progesterone, 17-hydroxyprogesterone, androstenedione, testosterone and dihydrotestosterone was developed and validated. For reference interval assessment, 280 well-characterized healthy subjects from the LifeLines cohort were selected, including 40 women using oral contraceptive pills (OCP). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120193010
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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.013DOI Listing
March 2019
2 Reads

What is the optimal time for measuring glucose concentration to detect steroid-induced hyperglycemia in patients with rheumatic diseases?

Clin Biochem 2019 May 23;67:33-39. Epub 2019 Mar 23.

Division of Rheumatology, Department of Internal Medicine, Eskişehir Osmangazi University, Eskişehir, Turkey.

Objective: Corticosteroids may cause hyperglycemia and diabetes mellitus (DM). Development of DM during long-term steroid use has been well studied; however, data regarding the short-term effects of steroid therapy are scarce. In this study, we aimed to detect the actual time of short-term steroid-induced hyperglycemia in patients without previous impaired glucose metabolism, and the ideal time (which day and in relation to meals) of glucose measurement. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.012DOI Listing
May 2019
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Verification of separate measurement procedures where analytical determinations influence the clinical interpretation of GFR: Iohexol quantitation by HPLC and LC-MS/MS.

Clin Biochem 2019 May 21;67:16-23. Epub 2019 Mar 21.

Advanced Research and Diagnostic Laboratory, University of Minnesota, Minneapolis, MN, USA; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, USA. Electronic address:

Background: The glomerular filtration rate (GFR) is monitored clinically to follow renal function of a patient. This is commonly performed using endogenous compounds, which estimate GFR (eGFR). However, several conditions exists which may confound or render the eGFR inaccurate. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.007DOI Listing
May 2019
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Droplet digital PCR improves urinary exosomal miRNA detection compared to real-time PCR.

Clin Biochem 2019 May 21;67:54-59. Epub 2019 Mar 21.

Keenan Biomedical Research Centre, Li Ka Shing Knowledge Institute, St Michael's Hospital, Toronto, ON, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada. Electronic address:

Object: Quantification of urinary miRNAs can be challenging especially for low abundance miRNAs. We aimed to optimize the quantification of urinary exosomal miRNAs and compare the performance efficiency between droplet digital PCR (ddPCR) and real-time quantitative PCR (qPCR).

Methods: We optimized a number of parameters for ddPCR such as annealing temperatures, annealing time and PCR cycle number. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.008DOI Listing
May 2019
2.229 Impact Factor

Consumption of Movantik™ (Naloxegol) results in detection of naloxone in the patient's urine evaluated by confirmatory urine drug testing.

Clin Biochem 2019 May 16;67:48-53. Epub 2019 Mar 16.

Elite Medical Laboratory Solutions, Tomball, TX, United States of America.

Background: Many patients on chronic opioid therapy suffer from constipation, one of the most common side effect of opioids. Movantik™ (naloxegol) is an opioid antagonist that is recently introduced in the market to treat opioid-induced constipation and contains naloxegol as the active ingredient. Naloxegol is a pegylated (polyethylene glycol-modified) derivative of α-naloxol. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.006DOI Listing

Acute kidney failure and discrepant values of urinary proteins: When the case is not "crystal clear".

Clin Biochem 2019 May 16;67:60-62. Epub 2019 Mar 16.

Nephrology Unit, Valencia University Clinic Hospital, Spain.

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.004DOI Listing
May 2019
4 Reads

Circulating NT-proBNP but not soluble corin levels were associated with preeclampsia in pregnancy-associated hypertension.

Clin Biochem 2019 May 16;67:12-15. Epub 2019 Mar 16.

Department of Cardiovascular Medicine, Cleveland Clinic, Cleveland, OH, United States of America; Center for Clinical Genomics, Cleveland Clinic, Cleveland, OH, United States of America; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, United States of America. Electronic address:

Background: Corin is a serine protease known to convert B-type natriuretic peptide (BNP) prohormone into BNP and its amino-terminal fragment (NT-proBNP). In mice lacking corin, high blood pressure and proteinuria were found at late gestational stages, with associated delayed trophoblast invasion and impaired spiral artery remodeling in the uterus. We hypothesize that both NT-proBNP and soluble corin elevation predict the presence of preeclampsia in pregnant patients with hypertension. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475478PMC
May 2019
1 Read

Long-term biological variation of high-sensitivity cardiac troponin T using minimal important differences and reference change values in stable outpatients with cardiovascular disease.

Clin Biochem 2019 May 11;67:7-11. Epub 2019 Mar 11.

Zentrum für Innere Medizin, Klinik für Kardiologie, Angiologie und Pneumologie, Universitätsklinikum Heidelberg, Germany.

Objective: To evaluate the long term biological variation of high-sensitivity cardiac troponin T (hs-cTnT) in stable outpatients with cardiovascular disease (CVD).

Methods: After applying 8 exclusion criteria to 965 patients, hs-cTnT was measured at index visit and at a 12-month interval in 169 stable outpatients presenting for routine follow-up visits for any CVD. Stability was defined as absence of any endpoint within the follow-up period. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.003DOI Listing
May 2019
1 Read

FGF23: Clinical usefulness and analytical evolution.

Clin Biochem 2019 Apr 7;66:1-12. Epub 2019 Mar 7.

Department of Laboratory Medicine, Cliniques Universitaires St-Luc, Université Catholique de Louvain, Brussels, Belgium; Pôle de recherche en endocrinologie, diabète et nutrition, Institut de recherche expérimentale et clinique, Cliniques universitaires Saint-Luc et Université catholique de Louvain, Bruxelles, Belgium. Electronic address:

Fibroblast Growth Factor 23 (FGF23) is a key hormone for the regulation of phosphate homeostasis. Over the past decades, FGF23 was the subject of intense research in the fields of nephrology and the cardiology. It presents a remarkable correlation with well-established biomarkers of cardiovascular disorders in both chronic kidney disease (CKD) and heart failure (HF) patients. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.002DOI Listing
April 2019
1 Read

Corrigendum to "Unexpectedly higher diazoxon hydrolysis by serum paraoxonase-1 in coronary heart disease" [Clinical Biochemistry 65 (2019) 21-23].

Clin Biochem 2019 Apr 6;66:106. Epub 2019 Mar 6.

Avda. Princip D'Espanya, 43892 Miami Playa, Tarragona, Spain. Electronic address:

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http://dx.doi.org/10.1016/j.clinbiochem.2019.03.001DOI Listing

Reference percentiles for paired arterial and venous umbilical cord blood gases: An indirect nonparametric approach.

Clin Biochem 2019 May 2;67:40-47. Epub 2019 Mar 2.

Services de Biochimie, Laboratoire de Biologie Médicale Multi-Sites, Hôpitaux Est-Sud-Nord-Edouard Herriot, Hospices Civils de Lyon (HCL), Lyon, France.

Background: Reference intervals for arterial and venous umbilical cord blood gas (UCBG) parameters are scarce, are mainly focused on pH, pO2, pCO2 and base deficit, and are usually assessed using parametric tests, despite a generally skewed data distribution. Here, the purpose is to determine reference percentiles for nine parameters of concomitant arterial and venous UCBG (CAV-UCBG) from neonates at birth, using nonparametric tests.

Methods: Results of CAV-UCBG, assayed over a 4. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.014DOI Listing
May 2019
2.229 Impact Factor

lncRNA RP5-916L7.2 correlates with advanced tumor stage, and promotes cells proliferation while inhibits cells apoptosis through targeting miR-328 and miR-939 in tongue squamous cell carcinoma.

Clin Biochem 2019 May 28;67:24-32. Epub 2019 Feb 28.

Department of Oral and Maxillofacial Surgery, Cangzhou Central Hospital, Cangzhou, China.

Background: This study aimed to investigate the correlation of lncRNA RP5-916L7.2 with tumor features of tongue squamous cell carcinoma (TSCC), and its effect on cells proliferation and apoptosis as well as its target miRNAs in TSCC cells.

Methods: 30 TSCC patients underwent surgery were consecutively enrolled, tumor tissue and paired adjacent tissue were obtained for lncRNAs determination. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.013DOI Listing

Plasma free metanephrines for diagnosis of neuroblastoma patients.

Clin Biochem 2019 Apr 26;66:57-62. Epub 2019 Feb 26.

Central Laboratory of Analyses, IRCCS Istituto Giannina Gaslini, Genoa, Italy. Electronic address:

Introduction: A substantial number of patients with neuroblastoma (NB) have increased excretion of catecholamines and metanephrines. Here, we have investigated the diagnostic role of plasma free metanephrines (PFM), metanephrine (MN), normetanephrine (NMN) and 3-methoxytyramine (3MT) for NB, the most common extra-cranial solid tumour in children.

Methods: PFM were quantified by using a commercial IVD-CE LC-MS/MS method on a TSQ Quantiva coupled to an Ultimate 3000. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183123
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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.012DOI Listing
April 2019
10 Reads

Identification of SAA and ACTB as potential biomarker of patients with severe HFMD using iTRAQ quantitative proteomics.

Clin Biochem 2019 May 25;67:1-6. Epub 2019 Feb 25.

Fujian Medical University Teaching Hospital, First Hospital of Putian, China.

Hand, foot and mouth disease (HFMD) is an infectious disease caused by a variety of enterovirus infections, and the most common types of virus infections are the newenterovirus71 (EV71) and coxsackievirus A group 16 (CoxA16). A small fraction of HFMD will cause further severe HFMD. A rapid and accurate diagnosis biomarker of severe HFMD is important for the timely treatment. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.011DOI Listing
May 2019
2 Reads

Evaluation of LeadCare Ultra® as an initial screen for elevated blood lead levels.

Clin Biochem 2019 Apr 19;66:95-99. Epub 2019 Feb 19.

Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, United States of America. Electronic address:

Objective: The LeadCare Ultra® (LCU) was compared to inductively coupled plasma mass spectrometry ICP-MS for use as a screening test for elevated blood lead levels (BLLs) in capillary samples from children.

Methods: During the validation, method comparisons between LCU and ICP-MS were analyzed to determine the bias above, near, and below the BLL cut-off of 5 μg/dL. Additionally, capillary samples that screened positive by LCU (above the 5 μg/dL cut-off) were compared to venous samples analyzed by ICP-MS for confirmatory testing. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.010DOI Listing

Explore potential plasma biomarkers of acute respiratory distress syndrome (ARDS) using GC-MS metabolomics analysis.

Clin Biochem 2019 Apr 16;66:49-56. Epub 2019 Feb 16.

Departmen of Emergency and Critical Care Medicine, The First Affiliated Hospital of Chongqing Medical University, China. Electronic address:

Objectives: The aim of this study was to analyse the metabolomics of patients with acute respiratory distress syndrome (ARDS) for the identification of metabolic markers with potential diagnostic and prognostic value.

Methods: The enrolled subjects included adult patients with ARDS that met the Berlin definition and healthy controls matched based on age, gender, and body mass index (BMI). Plasma samples were collected from 37 patients with ARDS and 28 healthy controls. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.009DOI Listing
April 2019
1 Read

The prognostic value of nitrotyrosine levels in coronary heart disease: long-term evaluation in the Acute Coronary Syndrome Registry Strategy (ERICO study).

Clin Biochem 2019 Apr 15;66:37-43. Epub 2019 Feb 15.

Center of Clinical and Epidemiological Research, Hospital Universitário, Universidade de Sao Paulo, Sao Paulo, Brazil. Electronic address:

Introduction: We aimed to analyze the association of nitrotyrosine (N-TYR) levels and long-term survival in an ongoing coronary heart disease (CHD) prospective cohort, the Acute Coronary Syndrome Registry Strategy (ERICO study).

Methods: N-TYR levels collected during acute and subacute phase from onset of acute coronary syndrome (ACS) symptoms (myocardial infarction and unstable angina) were evaluated in 342 patients. We calculated case-fatality rates (180-days, 1 year, 2 years and 4 years) and survival analyses up to 4 years using Kaplan-Meier curves and Cox regression with respective cumulative hazard ratios (95% confidence interval; 95%CI), according to N-TYR tertiles up to 4 years of follow-up. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.006DOI Listing

SLC22A3 is associated with lipoprotein (a) concentration and cardiovascular disease in familial hypercholesterolemia.

Clin Biochem 2019 Apr 14;66:44-48. Epub 2019 Feb 14.

Lipids, Nutrition and Cardiovascular Prevention Clinic, Montreal Clinical Research Institute, Québec, Canada; Department of Medicine, Division of Experimental Medicine, McGill University, Québec, Canada; Department of Medicine, Division of Medical Biochemistry, McGill University, Québec, Canada. Electronic address:

Background And Aims: Several clinical and genetic factors have been shown to modulate the cardiovascular risk in subjects affected by familial hypercholesterolemia (FH). Genome wide association studies (GWAS) in the general population have identified several single nucleotide polymorphisms (SNPs) significantly associated with the risk of cardiovascular disease (CVD). This include the rs2048327 variant in the SLC22A3 gene. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.008DOI Listing
April 2019
2 Reads

Correct implementation of the Hoffmann method.

Authors:
Daniel T Holmes

Clin Biochem 2019 Feb 13. Epub 2019 Feb 13.

University of British Columbia, Department of Pathology and Laboratory Medicine, Rm. G227 - 2211 Wesbrook Mall, Vancouver, BC V6T 2B5, Canada. Electronic address:

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.007DOI Listing
February 2019
1 Read

Principal component and correlation analysis of biochemical and endocrine markers in a healthy pediatric population (CALIPER).

Clin Biochem 2019 Apr 11;66:29-36. Epub 2019 Feb 11.

CALIPER Program, Department of Pediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada; Department of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada. Electronic address:

Background: Reference intervals (i.e. normative ranges) established from a healthy reference population are essential to accurately interpret disease biomarkers. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.004DOI Listing

Demethylation of the hypoxia induction factor 1 binding site of GPX3 at excess blood ammonia in propionic acidemia.

Clin Biochem 2019 Apr 10;66:100-102. Epub 2019 Feb 10.

Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Währinger Gürtel 18-20, Vienna, Austria.

Objectives: Elevated levels of metabolites such as ammonia and propionylcarnitine in propionic acidemia (PA) lead to an increased reactive oxygen species (ROS) production which could activate and stabilize the epigenetic regulated hypoxia-inducible factor-1α (HIF-1α). In order to evaluate the DNA methylation status of the HIF-1α binding site in PA, we investigated the antioxidant gluthatione peroxidase 3 gene (GPX3) promoter region.

Design And Methods: Using leukocyte DNA extracted from bloodspots collected 2-4 days after birth from diet free newborns, the cytosine phosphodiester bond guanine (CpG) dinucleotides of a HIF-1α binding site (CGTTTTTTACG) in the promoter region of GPX3 was retrospectively analysed. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.005DOI Listing

A false positive case of high-sensitivity cardiac troponin in a patient with acute chest pain: Analytical study of the interference.

Clin Biochem 2019 Apr 6;66:103-105. Epub 2019 Feb 6.

UOC Chimica, Biochimica e Biologia Molecolare Clinica, Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma, Italy; Università Cattolica del Sacro Cuore, Roma, Italy.

We report a case of a heterophile antibodies interference in a new high-sensitivity troponin commercial immunoassay (cTNIH Siemens), observed in a patient with possible acute coronary syndrome (ACS). The analytical interference was investigated with standard laboratories procedures. The false positive result was found with different troponin methods and kits. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.002DOI Listing
April 2019
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Cardiac troponin T concentrations and patient-specific risk of myocardial infarction using the novel PALfx parameter.

Clin Biochem 2019 Apr 4;66:21-28. Epub 2019 Feb 4.

Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, SE-41345 Gothenburg, Sweden. Electronic address:

Background: Myocardial infarction (MI) is more likely if the heart damage biomarker cardiac troponin T (cTnT) is elevated in a blood sample from a patient with chest pain. There is no conventional method to estimate the risk of MI at a specific cTnT concentration. The purpose of this study was to evaluate the performance of a novel method that converts cTnT concentrations to patient-specific risks of MI. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.003DOI Listing
April 2019
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Insufficient harmonization of antibiotics assays - Polish experience with an external quality assessment program in the years 2011-2018.

Clin Biochem 2019 Apr 4;66:91-94. Epub 2019 Feb 4.

Department of Monitored Therapy and Pharmacogenetics, Medical University of Gdansk, Debinki 7, 80-210 Gdansk, Poland.

Introduction: Treatment with vancomycin and gentamycin requires strict monitoring of its serum concentration for proper dosage optimization. This study aimed to assess the quality and the harmonization of antibiotics assays in Polish laboratories.

Materials And Methods: 413 results of vancomycin and 148 results of gentamycin assays obtained from Polish laboratories in 30 international external quality assessment (EQA) surveys carried out from March 2011 to May 2018 were analyzed. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.001DOI Listing

Impact of an electronic decision support rule on ESR/CRP co-ordering rates in a community health system and projected impact in the tertiary care setting and a commercially insured population.

Clin Biochem 2019 Apr 31;66:13-20. Epub 2019 Jan 31.

Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester 55905, MN, USA. Electronic address:

Introduction: Erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) are common laboratory assays used as markers of inflammation. ESR suffers from higher false positive and false negative rates than CRP. To that end, the American Board of Internal Medicine's (ABIM's) Choosing Wisely campaign has recommended against ESR testing for those with undiagnosed conditions in favor of CRP testing. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.009DOI Listing
April 2019
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Errors in measuring plasma free fatty acid concentrations with a popular enzymatic colorimetric kit.

Clin Biochem 2019 Apr 29;66:83-90. Epub 2019 Jan 29.

Division of Endocrinology, Diabetes and Metabolism, Endocrine Research Unit, Mayo Clinic, 200 1st Street SW, Rm 5-194 Joseph, Rochester, MN 55905, USA. Electronic address:

Objectives: Our goal was to test whether an enzymatic, colorimetric assay, the WAKO NEFA kit, provides information equivalent to liquid chromatography (LC) LC-based measures of free fatty acid (FFA).

Design & Methods: We reanalyzed nadir FFA samples from 109 volunteers from a previous study where we demonstrated that maximal suppression of FFA concentrations predicts metabolic abnormalities in humans; the results from the WAKO NEFA kit, which has been widely used for over three decades, could not replicate our findings. We conducted additional studies to directly compare results from this kit to our LC-mass spectrometry (LC/MS) method that was validated by our LC-UV detection method. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450739PMC
April 2019
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Fingerstick test quantifying humoral and cellular biomarkers indicative for M. leprae infection.

Clin Biochem 2019 Apr 26;66:76-82. Epub 2019 Jan 26.

Dept. of Infectious Diseases, Leiden University Medical Center, The Netherlands. Electronic address:

Objectives: New user-friendly diagnostic tests for detection of individuals infected by Mycobacterium leprae (M. leprae), the causative pathogen of leprosy, can help guide therapeutic and prophylactic treatment, thus positively contributing to clinical outcome and reduction of transmission. To facilitate point-of-care testing without the presence of phlebotomists, the use of fingerstick blood (FSB) rather than whole blood-derived serum is preferred. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183076
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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.007DOI Listing
April 2019
15 Reads

Aside from acute renal failure cases, are urinary markers of glomerular and tubular function useful in clinical practice?

Clin Biochem 2019 Mar 25;65:1-6. Epub 2019 Jan 25.

Sorbonne Université, UPMC Université Paris 06, INSERM UMRS_1155, Paris, France.; AP-HP, Service de Néphrologie et Dialyse, Hôpital Tenon, Paris, France.

The qualitative evaluation of proteinuria represents a crucial diagnostic step in clinical practice for the classification of renal diseases according to glomerular, tubulo-interstitial, mixed injury or related to monoclonal gammopathy. Combined with the quantitative evaluation, it also allows an assessment of the disease's severity and prognosis as well as the response to treatment. The development of the urine protein profile (UPP) combines specific urine protein assays on a urine spot analyzing glomerular protein markers such as albumin, transferrin and immunoglobulin G, and tubular markers such as alpha-1microglobulin and retinol binding protein, to generate a detailed quantitative and qualitative proteinuria assessment. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.006DOI Listing
March 2019
4 Reads

Proteome-wide onco-proteogenomic somatic variant identification in ER-positive breast cancer.

Clin Biochem 2019 Apr 23;66:63-75. Epub 2019 Jan 23.

Department of Laboratory Medicine and Pathobiology, University of Toronto, 1 King's College Circle, Toronto, ON M5S 1A8, Canada; Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Joseph and Wolf Lebovic Health Complex, 600 University Avenue, Toronto, ON M5G 1X5, Canada; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, 600 University Avenue, Toronto, ON M5G 1X5, Canada. Electronic address:

Background: Recent advances in mass spectrometric instrumentation and bioinformatics have critically contributed to the field of proteogenomics. Nonetheless, whether that integrative approach has reached the point of maturity to effectively reveal the flow of genetic variants from DNA to proteins still remains elusive. The objective of this study was to detect somatically acquired protein variants in breast cancer specimens for which full genome and transcriptome data was already available (BASIS cohort). Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.005DOI Listing
April 2019
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Unexpectedly higher diazoxon hydrolysis by serum paraoxonase-1 in coronary heart disease.

Clin Biochem 2019 Mar 16;65:21-23. Epub 2019 Jan 16.

Avda. Princip D'Espanya, 43892 Miami Playa, Tarragona, Spain. Electronic address:

Objectives: Low serum PON1 activities (paraoxon, phenyl-acetate or lactone substrates) are associated with coronary heart disease (CHD). We investigated the rate of diazoxon hydrolysis by PON1 in a population with CHD.

Design & Methods: Case- control study of 410 subjects with CHD and 274 controls. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.003DOI Listing
March 2019
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Circulating miR-26a, miR-106b, miR-107 and miR-133b stratify hepatocellular carcinoma patients according to their response to transarterial chemoembolization.

Clin Biochem 2019 Mar 15;65:45-52. Epub 2019 Jan 15.

Department of Cancer Biology, National Cancer Institute, Cairo University, Cairo, Egypt. Electronic address:

Background: A number of hepatocellular carcinoma (HCC) patients have developed resistance against transcatheter arterial chemoembolization (TACE) treatment. In this study, we aimed to develop a panel of microRNAs (miRs) biomarkers to predict clinical outcomes in HCC patients after TACE treatment.

Methods: The expression level of twenty miRs was evaluated in FFPE tissues collected from 33 HCC patients. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183091
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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397777PMC
March 2019
14 Reads

Impact of clinical sample handling and processing on ultra-low level measurements of plasma cytokines.

Clin Biochem 2019 Mar 8;65:38-44. Epub 2019 Jan 8.

Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, United States; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115, United States. Electronic address:

Objectives: In this study, we evaluated the impact of clinical sample handling and processing on IL-6, IL-10, IFNγ, and IL-2 measurements in plasma.

Design And Methods: We collected whole blood samples and analyzed various pre-analytical parameters. We assessed the following: 1) cytokine stability in whole blood that was stored over a ten-hour period at room temperature and 4 °C; 2) cytokine stability in plasma over 6 h; 3) vigorous sample handling including repeated dropping and transport through a pneumatic transport system; and 4) freeze-thaw stability of cytokines in plasma. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.001DOI Listing
March 2019
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Comparative accuracy of optical sensor-based wearable system for non-invasive measurement of blood glucose concentration.

Clin Biochem 2019 Mar 7;65:15-20. Epub 2019 Jan 7.

Maale Carmel Mental Health Center Affiliated to Bruce Rappaport Medical Faculty, Technion, Tirat Carmel, Israel.

Non-invasive biosensors for indirect evaluation of routinely-measured blood components by sweat analysis have broad potential clinical applications. This trial tested a wrist-borne non-invasive glucose monitor (NIGM) to measure blood glucose (BG) levels using photoplethysmographic (PPG) optical sensors. Our aim was to determine the accuracy of the device in comparison with a standard, invasive clinical method for blood glucose monitoring. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.014DOI Listing
March 2019
14 Reads