6,329 results match your criteria Clinical Biochemistry [Journal]


Two cases of Cushing's syndrome due to primary bilateral macronodular adrenal hyperplasia secondary to aberrant adrenal expression of hormone receptors.

Clin Biochem 2018 Jun 21. Epub 2018 Jun 21.

Servicio de Análisis Clínicos, Hospital Virgen de Los Lirios, Spain.

Objectives: Primary bilateral macronodular adrenal hyperplasia is an uncommon cause of endogenous Cushing's syndrome characterized by the presence of aberrant adrenal expression of ectopic receptors that regulate steroidogenesis by mimicking the events triggered by ACTH receptor activation. Receptors of this type have been described for several hormones. The aim of the study is to detect these receptors in two patients with ACTH-independent hypercortisolism by means of the application of a screening protocol. Read More

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Can copeptin and troponin T ratio predict final infarct size and myocardial salvage index in patients with ST-elevation myocardial infarction: A sub-study of the DANAMI-3 trial.

Clin Biochem 2018 Jun 19. Epub 2018 Jun 19.

Department of Cardiology, Herlev-Gentofte Hospital, Copenhagen, Denmark.

Background: Primary percutaneous coronary intervention (pPCI) is recommended in patients presenting with ST-elevation myocardial infarction (STEMI) within <12 h of symptom onset. However, patients-reported symptom duration is not always reliable. Cardiac specific troponin T (cTnT) and the endogenous stress marker copeptin have different temporal release patterns for myocardial infarction MI. Read More

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Focus on increased serum angiotensin-converting enzyme level: From granulomatous diseases to genetic mutations.

Clin Biochem 2018 Jun 18. Epub 2018 Jun 18.

Division of Cardiology, Cliniques Universitaires Saint-Luc, Université Catholique de Louvain, Brussels, Belgium; Pole of Cardiovascular Research, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.

Angiotensin I-converting enzyme (ACE) is a well-known zinc-metallopeptidase that converts angiotensin I to the potent vasoconstrictor angiotensin II and degrades bradykinin, a powerful vasodilator, and as such plays a key role in the regulation of vascular tone and cardiac function. Increased circulating ACE (cACE) activity has been reported in multiple diseases, including but not limited to granulomatous disorders. Since 2001, genetic mutations leading to cACE elevation have also been described. Read More

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Biochemical characterization of human tissue kallikrein 15 and examination of its potential role in cancer.

Clin Biochem 2018 Jun 18. Epub 2018 Jun 18.

Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada; Department of Clinical Biochemistry, University Health Network, Toronto, Canada; Mount Sinai Hospital, Lunenfeld-Tanenbaum Research Institute, Toronto, Canada; Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Canada. Electronic address:

Objective: Human tissue kallikrein 15 (KLK15) is the last cloned member of the KLK-related gene family. Despite being implicated in multiple cancers, its pathophysiological role remains unknown. We aimed to biochemically characterize KLK15 and preliminarily study its role in cancer. Read More

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June 2018
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Insulin resistance involvement in prevalence of familial dysbetalipoproteinemia in ε2ε2 subjects by Bayesian network modeling.

Clin Biochem 2018 Jun 18. Epub 2018 Jun 18.

Departments of Endocrinology, University of Groningen, University Medical Center Groningen, Groningen, RB 9700, The Netherlands.

Objective: Familial dysbetalipoproteinemia (FD) or Type III hyperlipoproteinemia is closely associated with the ε2ε2 genotype of the common APOE polymorphism although not all ε2 homozygotes develop FD indicating that additional factors play a role including insulin resistance (IR). The current study was undertaken to explore relationships and influences among factors, especially IR, that might elucidate FD progression pathways.

Methods: Bayesian network (BN) modeling, a probabilistic graphical exploratory data analysis tool that portrays relationships and influences among variables as simple diagrams, was applied to 52 e2e2 subjects. Read More

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Alzheimer's disease in the omics era.

Clin Biochem 2018 Jun 18. Epub 2018 Jun 18.

Tor Vergata University General Hospital, Dept. Experimental Medicine, Rome, Italy.

Recent progresses in high-throughput technologies have led to a new scenario in investigating pathologies, named the "Omics era", which integrate the opportunity to collect large amounts of data and information at the molecular and protein levels together with the development of novel computational and statistical tools that are able to analyze and filter such data. Subsequently, advances in genotyping arrays, next generation sequencing, mass spectrometry technology, and bioinformatics allowed for the simultaneous large-scale study of thousands of genes (genomics), epigenetics factors (epigenomics), RNA (transcriptomics), metabolites (metabolomics) and proteins(proteomics), with the possibility of integrating multiple types of omics data ("multi -omics"). All of these technological innovations have modified the approach to the study of complex diseases, such as Alzheimer's Disease (AD), thus representing a promising tool to investigate the relationship between several molecular pathways in AD as well as other pathologies. Read More

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Association of baseline big endothelin-1 level with long-term prognosis among cardiac resynchronization therapy recipients.

Clin Biochem 2018 Jun 15. Epub 2018 Jun 15.

State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Serum concentration of big endothelin-1 (ET-1) has prognostic significance in heart failure. However, its prognostic value in cardiac resynchronization therapy (CRT) recipients has not been well-characterized.

Methods: A cohort of 367 consecutive patients who received CRT between January 2010 and December 2015 were enrolled, and categorized into three groups according to baseline big ET-1 tertiles: big ET-1 ≤ 0. Read More

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Corrigendum to 'Validation of high-sensitivity performance for a United States Food and Drug Administration cleared cardiac troponin I assay' [Clin. Biochem. 56 (2018) 4-10].

Clin Biochem 2018 Jun 18. Epub 2018 Jun 18.

University of Maryland School of Medicine, Department of Pathology, 685 West Baltimore Street, MSTF Room 2-54, Baltimore, MD 21201, USA.

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Corrigendum to 'Two novel DNA variants associated with glucose-6-phosphate dehydrogenase deficiency found in Argentine pediatric patients' [Clin. Biochem. 49 (2016) 808-810].

Clin Biochem 2018 Jun 20. Epub 2018 Jun 20.

Servicio de Hematología-Oncología, Hospital de Pediatría "Prof. Dr. Juan P. Garrahan,", Combate de los Pozos 1881, C1245AAM Buenos Aires, Argentina. Electronic address:

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June 2018
2 Reads

Comparing the performance of CA 15-3 CSF to cytology in a cohort of patients with breast cancer leptomeningeal metastasis.

Clin Biochem 2018 Jun 12. Epub 2018 Jun 12.

Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, United States. Electronic address:

Background And Objective: Leptomeningeal metastasis (LM) can occur as a late manifestation of breast cancer and has traditionally been diagnosed by CSF cytology; however, cytology suffers from low sensitivity and it is believed that many cases of LM go undiagnosed. Some studies have suggested the use of CA 15-3 in CSF (CA 15-3 CSF) to aid in the detection of LM. The purpose of this study was to compare the performance of CA 15-3 CSF to cytology for the detection and treatment monitoring of breast cancer LM. Read More

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Evaluation of BD Vacutainer® Barricor™ blood collection tubes for routine chemistry testing on a Roche Cobas® 8000 Platform.

Clin Biochem 2018 Jun 6. Epub 2018 Jun 6.

Department of Laboratory Medicine, Saint John Regional Hospital, Saint John, NB, Canada; Department of Pathology, Dalhousie University, Halifax, NS, Canada. Electronic address:

Introduction: Barricor™ Vacutainers® are a novel non-gel separator blood collection tube. These tubes enable faster pre-analytical processing which could reduce turnaround time and be beneficial in an acute care setting. We sought to evaluate the bias, stability, and integrity of plasma generated from these tubes compared to Plasma Separator Tubes™ (PST) for 50 routine chemistry analytes on a Roche Cobas® 8000 analyzer. Read More

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Micro RNA as a potential blood-based epigenetic biomarker for Alzheimer's disease.

Clin Biochem 2018 Jun 6. Epub 2018 Jun 6.

Department of Epidemiology and Preventive Medicine, Monash University, Melbourne 3004, Victoria, Australia; Disease Epigenetics, Murdoch Childrens Research Institute, and The University of Melbourne, Parkville, 3052, Victoria, Australia; INSERM, U1061, Neuropsychiatrie, Recherche Clinique et Epidémiologique, Univ. Montpellier, Montpellier 34000, France.

As the prevalence of Alzheimer's disease (AD) increases, the search for a definitive, easy to access diagnostic biomarker has become increasingly important. Micro RNA (miRNA), involved in the epigenetic regulation of protein synthesis, is a biological mark which varies in association with a number of disease states, possibly including AD. Here we comprehensively review methods and findings from 26 studies comparing the measurement of miRNA in blood between AD cases and controls. Read More

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Reference intervals and diagnostic ranges for serum free κ and free λ immunoglobulin light chains vary by instrument platform: Implications for classification of patient results in a multi-center study.

Clin Biochem 2018 Jun 7. Epub 2018 Jun 7.

Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States. Electronic address:

Background: Serum free light chain (FLC) immunoglobulins are key biomarkers that aid in the diagnosis, prognosis and assessment of treatment response in patients with plasma cell disorders (PCD). Here we investigated the transference of manufacturer's reported κFLC, λFLC and κ to λ FLC reference intervals (RI) and established de novo FLC RI and diagnostic ranges on four instruments at three academic medical centers. In addition, we also compared the classification of patient FLC results using manufacturer's versus established RIs and diagnostic ranges. Read More

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June 2018
2 Reads

High-dose HOOK effect in urinary DcR2 assay in patients with chronic kidney disease.

Clin Biochem 2018 Jun 5. Epub 2018 Jun 5.

Department of Nephrology, Daping Hospital, Army Medical University, Chongqing 400042, China. Electronic address:

Background: Urinary DcR2 (uDcR2) is a biomarker for the early detection the tubulointerstitial injury (TII) in patients with chronic kidney disease (CKD), but the high-dose hook effect may lead to falsely low or even negative results when using an enzyme-linked immunosorbent assay (ELISA). This study aimed to investigate if the high-dose hook effect exists with ELISA testing, and to uncover a potential approach for reducing this effect.

Methods: 72 CKD patients were recruited and categorized into four groups based on TII scores. Read More

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Doubling immunochemistry laboratory testing efficiency with the cobas e 801 module while maintaining consistency in analytical performance.

Clin Biochem 2018 Jun 4. Epub 2018 Jun 4.

Roche Diagnostics GmbH, Mannheim, Germany.

Objectives: The new immunochemistry cobas e 801 module (Roche Diagnostics) was developed to meet increasing demands on routine laboratories to further improve testing efficiency, while maintaining high quality and reliable data.

Design And Methods: During a non-interventional multicenter evaluation study, the overall performance, functionality and reliability of the new module was investigated under routine-like conditions. It was tested as a dedicated immunochemistry system at four sites and as a consolidator combined with clinical chemistry at three sites. Read More

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June 2018
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Sources and performance criteria of uncertainty of reference measurement procedures.

Clin Biochem 2018 Jul 29;57:29-36. Epub 2018 May 29.

Centre for Metrological Traceability in Laboratory Medicine (CIRME), Dept. of Physiopatholgy and Transplantation, Università degli Studi di Milano, Milano, Italy.

Objective: This article wants to focus on the today available Reference Measurement Procedures (RMPs) for the determination of various analytes in Laboratory Medicine and the possible tools to evaluate their performance in the laboratories who are currently using them.

Methods: A brief review on the RMPs has been performed by investigating the Joint Committee for Traceability in Laboratory Medicine (JCTLM) database. In order to evaluate their performances, we have checked the organization of three international ring trials, i. Read More

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Effect of beef ingestion by humans on plasma concentrations of creatinine, urea, and cystatin C.

Clin Biochem 2018 May 26. Epub 2018 May 26.

Duke University Medical Center, Department of Pathology/Clinical Laboratories, Durham, NC, United States.

Purpose: The effect of eating meat on serum concentrations of creatinine has varied among previous reports, with some finding no effect and others finding 50-100% increases, which appears related to how the beef is cooked. For other analytes related to kidney function, urea is well known to increase following a protein meal, and the effect of eating meat on cystatin C concentrations has been studied once.

Methods: We had 32 participants eat a measured amount of cooked beef (5-6 or 10-12 oz; 142-170 or 284-340 g) and collected blood for measurements at 1 h before and immediately before eating beef, then at 1, 2, and 4 h after eating the beef. Read More

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Human kallikrein-related peptidase 12 (KLK12) splice variants discriminate benign from cancerous breast tumors.

Clin Biochem 2018 May 25. Epub 2018 May 25.

Department of Cellular Physiology, G. Papanicolaou Research Center of Oncology, "Saint Savvas" Cancer Hospital of Athens, Athens GR-11522, Greece. Electronic address:

Objectives: As kallikrein-related peptidase 12 (KLK12) has been implicated in the cancer progression and alternative splicing plays significant role in this disease, the aim of this study was to examine the expression profile and the clinical impact of the KLK12 splice variants in breast cancer.

Design And Methods: Total RNA was isolated and reverse transcripted from 141 tissues. Afterwards, quantitative real-time PCR were conducted, followed by the performance of the comparative CT (2-ΔΔCT) method for relative quantification, whilst their correlation with the clinicopathological features of breast malignancies were assessed by statistical analysis. Read More

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Should detectable cardiac troponin concentrations in a healthy population be the only criterion for classifying high-sensitivity cardiac troponin assays?

Authors:
Peter A Kavsak

Clin Biochem 2018 Jun 24;56:1-3. Epub 2018 May 24.

McMaster University, Hamilton, ON, Canada. Electronic address:

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False-positive amphetamine results on several drug screening platforms due to mexiletine.

Clin Biochem 2018 May 23. Epub 2018 May 23.

Department of Laboratory Medicine and Pathology, Mayo Clinic Rochester, United States.

Objective: False-positive urine drug of abuse screening (UDS) results can have serious implications in clinical practice, particularly when confirmation assay results are not immediately available to providers making medical decisions. Often it is not possible to identify the specific medication or other interfering compound that is responsible for the false-positive UDS result. Even when a potential interference is reported in the literature or package insert for one assay, the applicability to other UDS platforms/assays is often unknown. Read More

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Measuring estrogens in women, men, and children: Recent advances 2012-2017.

Clin Biochem 2018 May 22. Epub 2018 May 22.

Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT 84108, USA. Electronic address:

The measurement of estrogens is important for diagnosing and monitoring the health of women, men, and children. For example, for postmenopausal women or women undergoing treatment for breast cancer with aromatase inhibitors, the measurement of extremely low concentrations of estrogens in serum, especially estradiol, is problematic but essential for proper medical care. Achieving superb analytical sensitivity and specificity has been and continues to be a challenge for the clinical laboratory, but is a challenge that is being taken seriously. Read More

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Sperm DNA fragmentation in the total and vital fractions before and after density gradient centrifugation: Significance in male fertility diagnosis.

Clin Biochem 2018 May 21. Epub 2018 May 21.

Centre for Reproductive Medicine, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium.

Background: Sperm DNA fragmentation measured by different techniques make comparisons impossible due to lack of standardization. Induction of DNA damage after sperm preparation in the entire fraction has been observed on independent occasions but findings are not consistent.

Methods: Men presenting at a University hospital setup for infertility treatment. Read More

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Novel generations of laboratory instruments should not worsen analytical quality: The case of GEM Premier 5000.

Clin Biochem 2018 May 22. Epub 2018 May 22.

Research Centre for Traceability in Laboratory Medicine (CIRME), University of Milan, Milan, Italy; Clinical Pathology Unit, ASST Fatebenefratelli-Sacco, Milan, Italy.

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Analytical performance of an immunoassay to measure proenkephalin.

Clin Biochem 2018 May 18. Epub 2018 May 18.

Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, United States; Division of Cardiology, Mayo Clinic, Rochester, MN 55905, United States.

Background: Endogenous opioids, enkephalins, are known to increase with acute kidney injury. Since the mature pentapeptides are unstable, we evaluated the performance of an assay that measures proenkephalin 119-159 (PENK), a stable peptide formed concomitantly with mature enkephalins.

Methods: PENK assay performance was evaluated on two microtiterplate/chemiluminescence sandwich immunoassay formats that required 18 or 1 h incubation times. Read More

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Case report: Uncommon Sulfamethoxazole crystalluria.

Clin Biochem 2018 May 17. Epub 2018 May 17.

Clinical Chemistry Department, CHU de Liège, University of Liège, Belgium.

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How low can you go? Analytical performance of five automated testosterone immunoassays.

Clin Biochem 2018 May 12. Epub 2018 May 12.

Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT 84108, USA. Electronic address:

Background: Testosterone is commonly measured using immunoassays, yet concerns with the accuracy and quality of testing by these methods exist, particularly for low testosterone concentrations. Study objectives were to evaluate selective performance characteristics, including functional sensitivity (FS), of 5 automated immunoassays for total testosterone.

Methods: FS, imprecision, assay interference, limit of blank, linearity, and accuracy were assessed using the Abbott ARCHITECT i2000, SIEMENS ADVIA Centaur and IMMULITE 2000, Beckman Coulter DxI 800, and Roche MODULAR E170. Read More

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May 2018
2 Reads

The importance of low level QC for high sensitivity troponin assays.

Clin Biochem 2018 May 8. Epub 2018 May 8.

ACT Pathology, Garran, ACT 2605, Australia; Australian National University Medical School, Canberra, ACT 200, Australia.

Background: With the advent of the new high-sensitivity troponin assays, it is becoming critical to measure troponin accurately to low concentrations. To ensure assay performance is acceptable, appropriate QC must be run.

Methods: In addition to the routine use of commercial QC material, we prepared pools of human QC material with low troponin concentrations close to the limit of quantitation, and ran these regularly on our laboratory analysers. Read More

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Validation of high-sensitivity performance for a United States Food and Drug Administration cleared cardiac troponin I assay.

Clin Biochem 2018 Jun 9;56:4-10. Epub 2018 May 9.

University of Maryland School of Medicine, Department of Pathology, 685 West Baltimore Street, MSTF Room 2-54, Baltimore, MD 21201, USA.

Background: High-Sensitivity (hs) cardiac troponin (cTn) assays are categorized by two criteria: (i) cTn values above the limit of detection (LoD) for >50% of male and female healthy cohorts of ≥300 individuals; (ii) imprecision ≤10% total %CV for sex-specific 99th-percentile clinical decision values (CDVs). No documented hs-Tn assay has yet been FDA-cleared.

Methods: The PATHFAST cTnI-II assay's LoD was 2. Read More

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June 2018
1 Read

Different top-down approaches to estimate measurement uncertainty of whole blood tacrolimus mass concentration values.

Clin Biochem 2018 Jul 8;57:56-61. Epub 2018 May 8.

Laboratori de Referència d'Enzimologia Clínica, Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain.

Background: Values of mass concentration of tacrolimus in whole blood are commonly used by the clinicians for monitoring the status of a transplant patient and for checking whether the administered dose of tacrolimus is effective. So, clinical laboratories must provide results as accurately as possible. Measurement uncertainty can allow ensuring reliability of these results. Read More

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The potential role of a turbidimetric heart-type fatty acid-binding protein assay to aid in the interpretation of persistently elevated, non-changing, cardiac troponin I concentrations.

Clin Biochem 2018 May 8. Epub 2018 May 8.

Department of Medicine, McMaster University, Hamilton, ON, Canada; Division of Emergency Medicine, McMaster University, Hamilton, ON, Canada.

Background: Elevated and non-changing high-sensitivity cardiac troponin (hs-cTn) concentrations may suggest a process other than acute injury, possibly due to chronic condition(s) causing the elevation, an analytical error/interference or the formation of macrocomplexes. Heart-type fatty acid binding protein (H-FABP) might be useful in this setting to identify the etiology of abnormally high and non-changing cTn concentrations which could aid clinical decision making in the hospital setting.

Methods: We analytically validated the H-FABP assay (Randox) on the Abbott ARICHTECTc8000 platform, testing imprecision, linearity, stability, and matrix comparison. Read More

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High-sensitivity cardiac troponin I and risk of incident atrial fibrillation hospitalisation in an Australian community-based cohort: The Busselton health study.

Clin Biochem 2018 May 7. Epub 2018 May 7.

School of Population and Global Health, University of Western Australia, Crawley, Western Australia, Australia.

Objective: The identification of individuals at risk of atrial fibrillation (AF) remains a challenge. We investigated whether high-sensitive cardiac troponin I (hs-cTnI) is an independent predictor of incident atrial fibrillation (AF) hospitalisation in an Australian community-based cohort.

Methods: Serum hs-cTnI was measured in 1641 men and 2189 women in the Busselton Health Study 1994/1995 Cohort aged 25-84 years at baseline. Read More

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An approach to analytical validation and testing of body fluid assays for the automated clinical laboratory.

Clin Biochem 2018 May 5. Epub 2018 May 5.

Mayo Clinic, Department of Laboratory Medicine and Pathology, 200 First Street SW, Rochester, MN 55905, USA. Electronic address:

Background: Biochemical analysis of body fluids may lend insight into the pathogenesis of disease. Most commercially-available clinical laboratory methods are intended for blood-derived specimens and/or urine and laboratories must characterize the analytical performance of these methods for other specimen types such as body fluids. The aim of this work is to demonstrate one approach for characterizing analytical performance of assays for clinical analysis of body fluids. Read More

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May 2018
2 Reads

Triple test with tumor markers CYFRA 21.1, HE4, and ProGRP might contribute to diagnosis and subtyping of lung cancer.

Clin Biochem 2018 May 2. Epub 2018 May 2.

Altinbaş University School of Medicine, Department of Chest Diseases, Turkey.

Background And Aim: Early diagnosis and histological subtyping are important issues in the management of patients with lung cancer (LC). The aim of this study is to investigate the diagnostic value of a panel of serum tumor markers in newly diagnosed patients with LC.

Methods: Venous blood samples were collected from 99 patients with LC (42 adenocarcinoma, 35 squamous, and 22 small cell carcinoma) and 30 patients with benign lung disease. Read More

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Spurious testosterone laboratory results in a patient taking synthetic alkaline phosphatase (asfotase alfa).

Clin Biochem 2018 Apr 27. Epub 2018 Apr 27.

Department of Internal Medicine, Division Diabetes, Endocrinology, and Metabolism, University of Nebraska Medical Center, Omaha, NE, United States.

Objectives: We report a case of discordant total and free testosterone values in a patient with hypogonadism and juvenile hypophosphatasia after he initiated treatment with asfotase alfa, recombinant tissue non-specific alkaline phosphatase.

Methods: Total testosterone was evaluated using immunoassay pre and post initiation of therapy with asfotase alfa, and free testosterone was evaluated using radioimmunoassay and LC-MS/MS while on asfotase alfa therapy.

Results: Total testosterone measured by immunoassay was normal prior to therapy with asfotase alfa, and was low post initiation of therapy. Read More

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In response to a letter to Editor by Vivek Kumar Garg., et al. on our paper: "Dysregulation of serum NADPH oxidase1 and ferritin levels provides insights into diagnosis of Parkinson's disease". Hemmati-Dinarvand, M., et al. 2017; 50 (18):1087-1092.

Clin Biochem 2018 Jun 24;56:118-119. Epub 2018 Apr 24.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

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June 2018
1 Read

Aggrecanase degradation of type III collagen is associated with clinical knee pain.

Clin Biochem 2018 Apr 25. Epub 2018 Apr 25.

Fibrosis Biology, Biomarkers and Research, Nordic Bioscience, Herlev, Denmark.

There is a lack of biochemical markers for non-invasive and objective assessment of symptomatic osteoarthritis (OA). Aggrecanase activity has been shown to be associated with joint deterioration and symptomatic disease through the degradation of extracellular matrix proteins, such as type III collagen. Our study aimed to identify and develop a novel biomarker by measuring an aggrecanase-mediated type III collagen neoepitope, and correlate levels of this biomarker with OA joint pain. Read More

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Diagnostic and prognostic value of H-FABP in acute coronary syndrome: Still evidence to bring.

Clin Biochem 2018 Apr 24. Epub 2018 Apr 24.

Department of Biopathology and Medical Biotechnologies, Section of Clinical Biochemistry and Clinical Molecular Medicine, University of Palermo, Italy; Department of Laboratory Medicine, University-Hospital, Palermo, Italy. Electronic address:

The assessment of chest pain patients presenting to the emergency area (EA) is still a clinical challenge, as the majority of patients are not diagnosed with acute coronary syndrome (ACS). New generation high sensitivity c-Tn (hs-cTn) assays have showed better performances compared to the standard c-Tn. However, hs-Tn still presents some limitations. Read More

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April 2018
1 Read

Analytical validation and investigation on reference intervals of aldosterone and renin in Chinese Han population by using fully automated chemiluminescence immunoassays.

Clin Biochem 2018 Jun 22;56:89-94. Epub 2018 Apr 22.

Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Ave., 1095 Wuhan, Hubei, PR China. Electronic address:

Background: The aldosterone/renin ratio (ARR) is recommended to screen for primary aldosteronism (PA) in hypertension. We estimated fully automated chemiluminescence immunoassays (CLIA) for plasma aldosterone concentrations (PAC) and plasma direct renin concentrations (PRC) and investigated their reference intervals in Chinese Han population.

Methods: PAC and PRC were measured on a fully automated analyzer (LIAISON XL, DiaSorin, Italy). Read More

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Alpha-fetoprotein in pericardial, peritoneal, and pleural fluids: A body fluid matrix evaluation.

Clin Biochem 2018 Jun 21;56:109-112. Epub 2018 Apr 21.

ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA; Department of Pathology, University of Utah, 500 Chipeta Way, Salt Lake City, UT 84108, USA. Electronic address:

Objectives: Alpha-fetoprotein (AFP) measurement in pericardial, peritoneal (ascites), and pleural fluids is sometimes requested by clinicians as supportive evidence in the evaluation of suspected malignancy. As commercially available, Food and Drug Administration (FDA)-cleared AFP assays are not validated for these fluid types, laboratories must complete additional validation studies to comply with regulatory requirements for body fluid testing. The objective of this study was therefore to conduct a matrix evaluation for these body fluid types using the Beckman Access AFP assay on the UniCel DxI 800 immunoassay system. Read More

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June 2018
2 Reads

Order of draw practices in venous blood sampling at clinical biochemistry departments in the Danish health care system.

Clin Biochem 2018 Jun 21;56:113-116. Epub 2018 Apr 21.

Department of Technology, Faculty of Health and Technology, University College Copenhagen, Copenhagen, Denmark; National Food Institute, Technical University of Denmark, Lyngby, Denmark.

Background: Deviation in blood collection procedures is a central source of preanalytical variation affecting overall analytical and diagnostic precision. The order of draw of venous sampling is suspected to affect analytical results, in particular for coagulation analysis. Here we compare the procedures in venous blood sampling among clinical biochemistry departments to assess the uniformity of order of blood draw and adherence to international guidelines in the Danish health care system. Read More

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Commutability and traceability in EQA programs.

Clin Biochem 2018 Jun 20;56:102-104. Epub 2018 Apr 20.

RCPAQAP, St Leonards, Sydney, NSW, Australia.

Objectives: The concept of commutability of samples has focused laboratories on the importance of traceability. However, the critical role of External Quality Assurance (EQA) in achieving the primary role of traceability (i.e. Read More

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Elevated IgM against Nε-(Carboxyethyl)lysine-modified Apolipoprotein A1 peptide 141-147 in Taiwanese with Alzheimer's disease.

Clin Biochem 2018 Jun 20;56:75-82. Epub 2018 Apr 20.

School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan; Ph.D. Program in Medical Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan. Electronic address:

Objective: Advanced glycation end products (AGEs) are involved in the pathogenesis of Alzheimer's disease (AD). Specific AGEs and related autoantibodies may be early AD markers. Apolipoprotein A1 (ApoA1) and its post-translational modifications (PTMs) are associated with neurodegeneration and thus selected to test the hypothesis. Read More

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June 2018
1 Read

Mass spectrometric measurement of urinary kynurenine-to-tryptophan ratio in children with and without urinary tract infection.

Clin Biochem 2018 Jun 19;56:83-88. Epub 2018 Apr 19.

Departments of Pathology and Immunology, School of Medicine, Washington University, St. Louis, Missouri, USA; Departments of Pediatrics, School of Medicine, Washington University, St. Louis, Missouri, USA. Electronic address:

Background: Indoleamine-2,3-dioxygenase (IDO) catalyzes the first step of tryptophan (Trp) catabolism, yielding kynurenine (Kyn) metabolites. The kynurenine-to-tryptophan (K/T) ratio is used as a surrogate for biological IDO enzyme activity. IDO expression is increased during Escherichia coli urinary tract infection (UTI). Read More

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June 2018
1 Read

Reference materials for molecular diagnostics: Current achievements and future strategies.

Clin Biochem 2018 Jun 18;56:11-17. Epub 2018 Apr 18.

Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, China. Electronic address:

Background: Molecular diagnoses have become more widespread in many areas of laboratory medicine where qualitative or quantitative approaches are used to detect nucleic acids. The increasing number of assay methods and the targets for molecular diagnostics contribute to variability in the test results among clinical laboratories. Thus, reference materials (RMs) are required to enhance the comparability of results. Read More

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June 2018
1 Read

Potential diagnostic role of circulating MiRNAs in breast cancer: Implications on clinicopathological characters.

Clin Biochem 2018 Jun 19;56:47-54. Epub 2018 Apr 19.

Medical Oncology Department, National Cancer Institute, Cairo, Egypt.

Background: Circulating miRNAs are stable in body fluids and resembles their levels in cancer tissue/cells. They have been expressed in many cancers among them is breast cancer. Authors aimed to investigate the expression levels of three circulating oncomiRNAs (miRNA-21, miRNA-222 and miRNA-373) in serum samples as a minimally non-invasive method for early detection of breast cancer, and study their relation with clinicopathological characters. Read More

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June 2018
3 Reads

Serum retinol binding protein 4 and galectin-3 binding protein as novel markers for postmenopausal nonalcoholic fatty liver disease.

Clin Biochem 2018 Jun 19;56:95-101. Epub 2018 Apr 19.

Affiliated Hangzhou People's Hospital of Nanjing Medical University, Hangzhou, Zhejiang, China; Hangzhou Women's Hospital (Hangzhou Maternity and Child Health Care Hospital), Hangzhou, Zhejiang, China. Electronic address:

Objective: To investigate the differential protein expression before and after menopause in women with nonalcoholic fatty liver disease (NAFLD) and to explore novel markers for menopausal NAFLD.

Methods: Eight serum samples collected from pre- or post-menopausal women with NAFLD were analysed by iTRAQ 2D-LC-MS/MS. Two protein candidates were selected and verified by enzyme-linked immunosorbent assay (ELISA) in serum samples collected from a one hundred and fifty-three female population subsequently, including 51 in post-menopausal status with NAFLD, 41 in pre-menopausal with NAFLD, 19 healthy individuals in post-menopausal status and 42 healthy pre-menopausal women. Read More

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June 2018
1 Read

Ensuring suitable quality of clinical measurements through design.

Clin Biochem 2018 Jul 17;57:48-55. Epub 2018 Apr 17.

Abbott, Abbott Park, IL, USA. Electronic address:

To design and deliver high quality, safe and effective products, manufacturers of in vitro diagnostic (IVD) products follow a structured, traceable process for controlling the uncertainty of results reported from their measurement systems. This process and its results however, are not often shared in detail with those outside of the manufacturing company. The objective of this paper is to facilitate discussion by describing some of the best practices used during the IVD design and development process, highlighting some design challenges manufacturers face, and to offer ideas for how IVD manufacturers and laboratories could work together to drive further improvement to public health. Read More

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July 2018
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Assessment of hair cortisol as a potential biomarker for possible adrenal suppression due to inhaled corticosteroid use in children with asthma: A retrospective observational study.

Clin Biochem 2018 Jun 16;56:26-32. Epub 2018 Apr 16.

Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada; Maccabi-Kahn Research Institute, Tel Aviv, Israel.

Background: Inhaled corticosteroids (ICS) are the recommended long-term control therapy for asthma in children. However, concern exists regarding potential adrenal suppression with chronic ICS use. Our pilot study reported that hair cortisol in children was 50% lower during ICS therapy than prior to therapy, suggestive of adrenal suppression. Read More

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June 2018
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Serum concentrations of folate vitamers in patients with a newly diagnosed prostate cancer or hyperplasia.

Clin Biochem 2018 Jun 16;56:41-46. Epub 2018 Apr 16.

Department of Clinical Chemistry and Laboratory Medicine, Saarland University Hospital, D-66421 Homburg/Saar, Germany. Electronic address:

Background: Folate is required for synthesis of methyl groups and DNA in growing cells. The association between folate and prostate cancer (PCa) is not conclusive.

Methods: We investigated concentrations of folate vitamers, S-adenosylhomocysteine (SAH) and S-adenosylmethionine (SAM) in blood of men with PCa (n = 129) or benign prostatic hyperplasia (BPH) (n = 73) who were recruited just after the first diagnosis. Read More

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June 2018
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Systemic redox biomarkers and their relationship to prognostic risk markers in autosomal dominant polycystic kidney disease and IgA nephropathy.

Clin Biochem 2018 Jun 12;56:33-40. Epub 2018 Apr 12.

Department of Medicine, Stavanger University Hospital, Stavanger, Norway. Electronic address:

Background: Oxidative stress is evident from an early stage in chronic kidney disease (CKD). Therefore, we investigated redox biomarkers in polycystic kidney disease (ADPKD) and IgA nephropathy (IGAN).

Methods: This is a case-control study with three groups: ADPKD (n = 54), IGAN (n = 58) and healthy controls (n = 86). Read More

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June 2018
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