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    6181 results match your criteria Clinical Biochemistry [Journal]

    1 OF 124

    Neutrophil to lymphocyte ratio predict mortality and major adverse cardiac events in acute coronary syndrome: A systematic review and meta-analysis.
    Clin Biochem 2017 Nov 11. Epub 2017 Nov 11.
    Department of Vascular Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address:
    Objectives: Neutrophil to lymphocyte ratio (NLR) might be associated with the mortality or major adverse cardiac events (MACEs) in acute coronary syndrome (ACS) patients. We performed a meta-analysis to evaluate the correlation between NLR and mortality/MACEs in ACS.

    Methods: We assessed clinical trials through Pubmed, EMBASE, the Cochrane Library and Web of science in investigating the association between NLR and mortality/MACEs in ACS patients up to August 15, 2017. Read More

    Serum reference interval of ARCHITECT alpha-fetoprotein in healthy Chinese Han adults: Sub-analysis of a prospective multi-center study.
    Clin Biochem 2017 Nov 10. Epub 2017 Nov 10.
    Department of Laboratory Medicine, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710000, China. Electronic address:
    Objectives: Alpha-fetoprotein (AFP) has been widely used in clinical practice for decades. However, large-scale survey of serum reference interval for ARCHITECT AFP is still absent in Chinese population. This study aimed to measure serum AFP levels in healthy Chinese Han subjects, which is a sub-analysis of an ongoing prospective, cross-sectional, multi-center study (ClinicalTrials. Read More

    Clinical significance of the serum biomarker index detection in children with Henoch-Schonlein purpura.
    Clin Biochem 2017 Nov 9. Epub 2017 Nov 9.
    School of Medical Laboratory, Tianjin Medical University, No.1 Guangdong Road, Hexi District, Tianjin 300203, China. Electronic address:
    Objective: To explore a panel of serum biomarkers for laboratory diagnosis of pediatric Henoch-Schönlein purpura (HSP).

    Methods: The blood white blood cells (WBC) and serum levels of serum amyloid A (SAA), interleukin 6 (IL-6), immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), immunoglobulin E (IgE), C-reactive protein (CRP), complement component 3 (C3), complement component 4 (C4), and ASO (anti-streptolysin O) were detected in 127 patients with Henoch-Schonlein purpura (HSP), 110 cases of septicemia patients, and 121 healthy volunteers. The diagnostic ability of biomarkers selected from HSP and septicemia patients was analyzed by ROC curve. Read More

    Comparison of fasting and non-fasting lipid profiles in a large cohort of patients presenting at a community hospital.
    Clin Biochem 2017 Nov 9. Epub 2017 Nov 9.
    Centre Hospitalier Universitaire de Québec-Université Laval, Québec, QC, Canada.
    Objective: To compare the fasting and non-fasting lipid profile including ApoB in a cohort of patients from a community setting. Our purpose was to determine the proportion of results that could be explained by the known biological variation in the fasting state and to examine the additional impact of non-fasting on these same lipid parameters.

    Methods: 1093 adult outpatients with fasting lipid requests were recruited from February to September 2016 at the blood collection sites of the Moncton Hospital. Read More

    Parathyroid hormone related protein concentration in human serum and CSF correlates with age.
    Clin Biochem 2017 Nov 6. Epub 2017 Nov 6.
    NMS Labs, Willow Grove, PA, United States.
    Background: Parathyroid Hormone-Related Protein (PTHrP) is involved in intracellular calcium (Ca) regulation, and has been demonstrated to participate in regulation of Ca in brain cells, activation of neurons, and modulation of pain. However, there are conflicting reports regarding the presence of PTHrP in CSF.

    Design And Methods: PTHrP and Ca were quantified in paired CSF and serum samples using mass spectrometry-based methods. Read More

    Evaluation of the Roche® Elecsys and the Diasorin® Liaison S100 kits in the management of mild head injury in the emergency room.
    Clin Biochem 2017 Nov 7. Epub 2017 Nov 7.
    Emergency Department, Ghent University Hospital, Ghent, Belgium.
    Introduction: The aim of this single-center prospective study is to compare two commercially available S100ß kits (the Roche® Elecsys and the Diasorin® Liaison S100 kits) in terms of analytical and clinical performances in a population admitted in the emergency room for mild traumatic brain injury (mTBI).

    Material And Method: 110 patients were enrolled from September 2014 to May 2015. Blood sample draws were performed within 3h after head trauma and the study population was split into pediatric and adult subpopulations (>18years of age). Read More

    Next generation sequencing as a follow-up test in an expanded newborn screening programme.
    Clin Biochem 2017 Oct 27. Epub 2017 Oct 27.
    University Medical Centre Ljubljana, University Children's Hospital, Bohoriceva 20, Ljubljana, Slovenia; University of Ljubljana, Faculty of Medicine, Korytkova ulica 2, Ljubljana, Slovenia. Electronic address:
    Objectives: Contrary to many western European countries, most south-eastern European countries do not have an expanded newborn screening (NBS) program using tandem mass spectrometry. This study would represent one of the first expanded NBS studies in south-eastern Europe and will enable the estimation of the incidences of IEM in Slovenia. We proposed an expanded NBS approach including next-generation sequencing (NGS) as a confirmational analysis. Read More

    Reproducibility of serum oxysterols and lanosterol among postmenopausal women: Results from EPIC-Heidelberg.
    Clin Biochem 2017 Nov 3. Epub 2017 Nov 3.
    Division of Cancer Epidemiology, German Cancer Research Center (DKFZ), Heidelberg, Germany. Electronic address:
    Background: Circulating oxysterols have been proposed as biological markers of disease risk. However, within-person reproducibility of circulating oxysterols over time is not well established.

    Methods: We evaluated the one-year reproducibility of 11 oxysterols and lanosterol among 30 postmenopausal women with repeat blood samples in the European Prospective Investigation into Cancer and Nutrition (EPIC) - Heidelberg, Germany cohort. Read More

    Moving standard deviation and moving sum of outliers as quality tools for monitoring analytical precision.
    Clin Biochem 2017 Oct 26. Epub 2017 Oct 26.
    Department of Laboratory Medicine, National University Hospital, Singapore; Biomedical Institute for Global Health Research and Technology, National University of Singapore, Singapore. Electronic address:
    Introduction: An increase in analytical imprecision (expressed as CVa) can introduce additional variability (i.e. noise) to the patient results, which poses a challenge to the optimal management of patients. Read More

    Combining high-sensitivity cardiac troponin and B-type natriuretic peptide in the detection of inducible myocardial ischemia.
    Clin Biochem 2017 Nov 8. Epub 2017 Nov 8.
    Cardiovascular Research Institute Basel (CRIB), Department of Cardiology, University Hospital Basel, University Basel, Switzerland. Electronic address:
    Background: Single biomarker approaches provide only moderate accuracy in the non-invasive detection of exercise-induced myocardial ischemia. We therefore assessed the combination of the two most promising single biomarkers: high-sensitivity cardiac troponin I (hs-cTnI) and B-type natriuretic peptide (BNP).

    Methods: Consecutive patients with suspected myocardial ischemia referred to stress myocardial perfusion single-photon emission tomography imaging (MPI) were enrolled. Read More

    Macro-pro-B-type natriuretic peptide (proBNP) and hidden macro-N-terminal proBNP: Case report.
    Clin Biochem 2017 Nov 2. Epub 2017 Nov 2.
    Department of Cardiovascular Medicine, Kyoto University Graduate School of Medicine, Japan.
    B-type natriuretic peptide (BNP) is a cardiac hormone widely used as a biomarker for heart failure. Here, we present the first report of extremely high levels of immunoreactive BNP caused by formation of macro-proBNP. A 70-year-old woman with left ventricular hypertrophy and normal systolic function presented with extremely high plasma levels of BNP (35,374pg/ml) and N-terminal proBNP (NT-proBNP; 30,600pg/ml). Read More

    The copeptin response after physical activity is not associated with cardiac biomarkers or asymptomatic coronary artery disease: The North Sea Race Endurance Exercise Study (NEEDED) 2013.
    Clin Biochem 2017 Oct 26. Epub 2017 Oct 26.
    Cardiology Department, Stavanger University Hospital, Stavanger, Norway; Department of Electrical Engineering and Computer Science, University of Stavanger, Norway.
    Background: Copeptin concentrations increase both during acute coronary syndrome and following physical exercise. The relationship between copeptin increase following physical exercise and coronary artery disease (CAD) is uncertain. The aim of this study was to 1) describe the copeptin response following strenuous physical exercise, and 2) investigate the determinants of exercise induced copeptin concentrations, particularly in relation to cardiac biomarkers and CAD. Read More

    Monoclonal protein reference change value as determined by gel-based serum protein electrophoresis.
    Clin Biochem 2017 Oct 24. Epub 2017 Oct 24.
    Division of Biochemistry, The Ottawa Hospital, Department of Pathology and Laboratory Medicine, University of Ottawa, Ottawa, Ontario, Canada. Electronic address:
    Introduction: The International Myeloma Working Group recommendations for monitoring disease progression or response include quantitation of the involved monoclonal immunoglobulin. They have defined the minimum change criteria of ≧25% with an absolute change of no <5g/L for either minimal response or progression. Limited evidence is available to accurately determine the magnitude of change in a monoclonal protein to reflect a true change in clinical status. Read More

    Fibroblast growth factor 23 in patients with acute dyspnea: Data from the Akershus Cardiac Examination (ACE) 2 Study.
    Clin Biochem 2017 Oct 24. Epub 2017 Oct 24.
    Division of Medicine, Akershus University Hospital, Lørenskog, Norway; Center for Heart Failure Research, University of Oslo, Oslo, Norway. Electronic address:
    Background: Circulating fibroblast growth factor 23 (FGF23) concentrations have been linked to left ventricular remodeling and unfavorable cardiovascular outcomes, but whether FGF23 is associated with heart failure (HF) diagnosis and outcome in unselected patients with dyspnea is unknown. Accordingly, we assessed the diagnostic and prognostic properties of FGF23 in patients presenting to the emergency department with acute dyspnea.

    Methods And Results: FGF23 was measured in 314 patients admitted with acute dyspnea and the diagnostic and prognostic merit was compared to that of N-terminal pro-B-type natriuretic peptide (NT-proBNP). Read More

    Candidate recommendations for protein electrophoresis reporting from the Canadian Society of Clinical Chemists Monoclonal Gammopathy Working Group.
    Clin Biochem 2017 Oct 20. Epub 2017 Oct 20.
    Grand River Hospital, Kitchener, ON, Canada.
    Protein electrophoresis is commonly used as an aid in the diagnosis of monoclonal gammopathies and is performed in many laboratories in Canada and throughout the world. However, unlike many other diagnostic tests, there is limited guidance for standardization and neither guidance nor specific recommendations for clinical reporting of serum (SPE) or urine (UPE) protein electrophoresis and immunotyping available in the literature. Therefore, a Canadian effort was undertaken to recommend standards that cover all aspects of clinical reporting with an ultimate goal towards reporting standardization. Read More

    Clustered interventions to reduce inappropriate duplicate laboratory tests in an Irish tertiary hospital.
    Clin Biochem 2017 Oct 19. Epub 2017 Oct 19.
    Centre for Interventions in Infection, Inflammation & Immunity (4i) and Graduate-Entry Medical School, University of Limerick, Limerick, Ireland. Electronic address:
    Background: There is increasing emphasis on understanding the rate, and avoidable costs, of inappropriate laboratory testing in hospitals, especially associated with duplication of tests following transfer of patients from one hospital to another. While studies of inappropriate testing have been reported previously, there are no published data relevant to Ireland.

    Aims: To determine the baseline rate of inappropriate testing for a subset of clinical parameters, specifically, full blood counts (FBC), biochemistry profiles (Bio) and coagulation (Coag) screens for geriatric patients transferring to and from University Hospital Limerick (UHL). Read More

    Diagnostic value of alpha-1-fetoprotein (AFP) as a biomarker for hepatocellular carcinoma recurrence after liver transplantation.
    Clin Biochem 2017 Oct 17. Epub 2017 Oct 17.
    Department of Clinical Pharmacology, University Medical Center Göttingen, Kreuzbergring 36, 37075 Göttingen, Germany. Electronic address:
    Background: Alpha-1-fetoprotein (AFP) is used to monitor progression, evaluate response to therapy and predict recurrence of hepatocellular carcinoma (HCC) in liver transplantation (LTx) patients. To date, the diagnostic value of serum AFP determinations for detecting tumor recurrence in HCC patients after LTx is unclear.

    Objective: A retrospective, single-center, cross-sectional, non-interventional study was performed with the objective of determining post-transplant cut-off AFP values for detecting HCC recurrence post LTx. Read More

    External validation of heart-type fatty acid binding protein, high-sensitivity cardiac troponin, and electrocardiography as rule-out for acute myocardial infarction.
    Clin Biochem 2017 Oct 18. Epub 2017 Oct 18.
    Faculty of Medicine, The University of Queensland, Brisbane, Australia; Department of Emergency Medicine, Royal Brisbane and Women's Hospital, Brisbane, Australia; Faculty of Health, Queensland University of Technology, Australia. Electronic address:
    Objective: To externally validate a clinical decision rule incorporating heart fatty acid binding protein (h-FABP), high-sensitivity troponin (hs-cTn) and electrocardiogram (ECG) for the detection of acute myocardial infarction (AMI) on presentation to the Emergency Department. We also investigated whether this clinical decision rule improved identification of AMI over algorithms incorporating hs-cTn and ECG only.

    Design And Methods: This study included data from 789 patients from the Brisbane ADAPT cohort and 441 patients from the Christchurch TIMI RCT cohort. Read More

    Effect of storage time, temperature, antioxidant and thawing on fatty acid composition of plasma, serum and red blood cells - A pilot biobank study.
    Clin Biochem 2017 Oct 18. Epub 2017 Oct 18.
    National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029, Nordnes, N-5817 Bergen, Norway.
    Background: It studies on the factors that affect the stability of fatty acid profiles from human blood specimens are generally performed by evaluating the effect of a single factor on an individual fatty acid and excluding a considerable amount of data from the total fatty acid profiles.

    Methods: The stability of fatty acids from plasma, serum and red blood cells (RBC) was evaluated in terms of time, temperature, antioxidant and thawing. The fatty acids were methylated and analyzed by gas chromatography. Read More

    Stability of proinsulin in whole blood.
    Clin Biochem 2017 Oct 16. Epub 2017 Oct 16.
    Diabetes Research Unit Cymru, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, UK.
    Proinsulin, the precursor for insulin, is secreted in higher concentrations when β-cells are under stress and previous studies have shown that elevated proinsulin could be used as a marker for individuals in a pre-diabetic state. The aim of this study was to assess the stability of proinsulin across a wide concentration range (3-882 and 2-187pmol/L; total and intact proinsulin respectively) in whole blood to determine whether it could be used in routine clinical care. 51 subjects (26 normal glucose tolerance, 17 impaired glucose tolerance and 8 type 2 diabetes) had blood taken into EDTA tubes at 0, 60 & 120min following a glucose load. Read More

    Pre-operative and post-operative changes in CRP and other biomarkers sensitive to inflammatory status in patients with severe obesity undergoing laparoscopic sleeve gastrectomy.
    Clin Biochem 2017 Oct 16. Epub 2017 Oct 16.
    Surgery of the Faculty of Medicine, Memorial University, St. John's, NL A1B 3V6, Canada.
    Introduction: C-reactive protein (CRP) is often elevated in patients living with severe obesity (BMI≥35kg/m(2)). However, there is limited information on how CRP, and other inflammation responsive biomarkers, change in response to weight loss following laparoscopic sleeve gastrectomy (LSG). We studied how CRP, ferritin and albumin change following LSG surgery in relation to obesity, metabolic syndrome (MetS) ATPIII risk components and diabetes mellitus (DM). Read More

    Description of analytical method and clinical utility of measuring serum 7-alpha-hydroxy-4-cholesten-3-one (7aC4) by mass spectrometry.
    Clin Biochem 2017 Oct 16. Epub 2017 Oct 16.
    C.E.N.T.E.R, Mayo Clinic, Rochester, MN, United States.
    Background: Imbalance of bile acids (BA) homeostasis in the gastrointestinal tract can lead to chronic diarrhea or constipation when BA in the colon are in excess or low, respectively. Since both disturbances of bowel function can result from other etiologies, identifying BA imbalance is important to tailor treatment strategies. Serum concentrations of 7-alpha-hydroxy-4-cholesten-3-one (7aC4), a precursor in bile acid synthesis, reflect BA homeostasis. Read More

    Korean pediatric reference intervals for FT4, TSH, and TPO Ab and the prevalence of thyroid dysfunction: A population-based study.
    Clin Biochem 2017 Oct 9. Epub 2017 Oct 9.
    Department of Pediatrics, Chungnam National University College of Medicine, Daejeon, South Korea. Electronic address:
    Background: The appropriate age-related reference intervals for thyroid-stimulating hormone (TSH) and free thyroxine (FT4) are important in interpreting the results of thyroid function tests in children and adolescents. However, these reference intervals are scanty. This study aimed to establish the reference intervals for FT4, TSH, and thyroid peroxidase antibody (TPO Ab), and identify the epidemiological prevalence of thyroid dysfunction in a Korean pediatric population. Read More

    Serum interleukin measurement may help identify thyroid cancer patients with active disease.
    Clin Biochem 2017 Oct 5. Epub 2017 Oct 5.
    Laboratory of Cancer Molecular Genetics, Faculty of Medical Sciences (FCM), University of Campinas (Unicamp), 126, Tessalia Vieira de Camargo St., Campinas, SP, Brazil.
    Background: Investigate the clinical utility of serum interleukin dosages of IL-2, IL-2R, IL-4, IL-6, IL-6R, IL-8, IL-10 and IL-12 in the diagnosis and characterization of patients with DTC. In particular, verify ILs utility in the identification of individuals who are evolving disease-free or with the active disease.

    Methods: We evaluated 200 patients with malignant nodules (100 patients disease-free and 100 patients with recurrence/active disease); 60 benign nodules and 100 healthy controls, serum levels were assessed by ELISA. Read More

    Simultaneous determination of fluoxetine and norfluoxetine in dried blood spots using high-performance liquid chromatography-tandem mass spectrometry.
    Clin Biochem 2017 Oct 5. Epub 2017 Oct 5.
    Laboratory of Analytical Toxicology, Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, RS, Brazil. Electronic address:
    Background: Therapeutic drug monitoring (TDM) of the widely prescribed antidepressant fluoxetine (FLU) is recommended in certain situations, such as occurrence of toxicity, inadequate response or suspect of poor adherence. Dried blood spot (DBS) sampling is an increasingly studied alternative for TDM, particularly for outpatients, due to its ease of collection and inherent stability.

    Objectives: The aim of this study was to develop and validate an LC-MS/MS assay for the simultaneous quantification of FLU and norfluoxetine (NFLU) in DBS. Read More

    Peaks and tails: Evaluation of irregularities in capillary serum protein electrophoresis.
    Clin Biochem 2017 Sep 28. Epub 2017 Sep 28.
    Medica (Medizinische Laboratorien Dr. F. Kaeppeli AG), Zürich, Switzerland.
    The increased analytical sensitivity of capillary electrophoresis detects additional irregularities that are suspicious for a monoclonal component. This is most noticeable in the beta-1-, beta-2- and gamma-globulin fractions. The causes of non-monoclonal irregularities are manifold, but are rarely reported back to the ordering physician. Read More

    Is cathelicidin a novel marker of diabetic microangiopathy in patients with type 1 diabetes?
    Clin Biochem 2017 Sep 28. Epub 2017 Sep 28.
    Department of Internal Medicine and Diabetology, Poznan University of Medical Sciences, Raszeja Hospital, Mickiewicza 2, 60-834 Poznan, Poland.
    Aim: The aim was to evaluate the relationship between higher serum cathelicidin levels with the occurrence of chronic microangiopathic complications in patients with diabetes mellitus type 1 (DM1).

    Methods: The study group consisted of 62 patients with DM1 (35 men), aged 30 (24-38) years and with duration of DM1 12 (9-17) years. Patients were divided into two groups depending on the level of cathelicidin, with cut-off point 24. Read More

    Beta-glucuronidase activity in dried blood spots: Reduced technique with biochemical parameters determined.
    Clin Biochem 2017 Sep 27. Epub 2017 Sep 27.
    Graduate Program in Biological Sciences: Biochemistry, Department of Biochemistry, Institute of Basic Healthcare Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
    Introduction: Mucopolysaccharidoses (MPS) occur due to deficiency in the activity of enzymes that catalyze the breakdown of glycosaminoglycans. MPS VII is caused by deficiency of the beta-glucuronidase enzyme (GUSB).

    Objectives: This study aimed to enhance the technique to measure GUSB activity by reducing the amount of reagents and the size of the DBS, as well as to determine some biochemical parameters of enzyme of healthy individuals. Read More

    Effect of a low glycemic index diet versus a high-cereal fibre diet on markers of subclinical cardiac injury in healthy individuals with type 2 diabetes mellitus: An exploratory analysis of a randomized dietary trial.
    Clin Biochem 2017 Sep 25. Epub 2017 Sep 25.
    Clinical Nutrition and Risk Factor Modification Centre, St. Michael's Hospital, Toronto, Ontario, Canada; Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada; Li Ka Shing Knowledge Institute, St. Michael's Hospital, Toronto, Ontario, Canada; Division of Endocrinology and Metabolism, St. Michael's Hospital, Toronto, Ontario, Canada. Electronic address:
    Background: Markers of subclinical cardiac injury are elevated in individuals with type 2 diabetes mellitus (T2DM) compared to healthy individuals. Low glycemic index (LGI) diets may improve both diabetes and cardiovascular risk but their effects on cardiac injury and fibrosis have not been previously studied. To test the effect of a LGI diet on markers of subclinical cardiac injury and fibrosis, we assessed the effect of a LGI compared with a high-cereal fibre diet on high-sensitivity cardiac troponin I (hs-cTnI) and galectin-3 in otherwise healthy individuals with T2DM in an exploratory analysis of a completed randomized trial. Read More

    Reference change values of plasma and urine NGAL in cardiac surgery with cardiopulmonary bypass.
    Clin Biochem 2017 Sep 23. Epub 2017 Sep 23.
    Département d'Anesthésie-Réanimation, Hôpital Bichat-Claude Bernard, Assistance Publique des Hôpitaux de Paris (AP-HP), Paris, France. Electronic address:
    Objective: As with any biomarker, interpretation of changes of NGAL concentration must consider its variability in a specific clinical setting. The aim of this study was to calculate the reference change value (RCV) and the index of individuality (II) of plasma and urine NGAL in the context of coronary artery bypass graft surgery with cardiopulmonary bypass, in patients without postoperative acute kidney injury.

    Methods: This prospective single-center observational study included patients with a preoperative glomerular filtration rate of >30mlmin(-1) 1. Read More

    Influence of diurnal variation and fasting on serum iron concentrations in a community-based population.
    Clin Biochem 2017 Sep 23. Epub 2017 Sep 23.
    Department of Pathology and Laboratory Medicine, Cumming School of Medicine, University of Calgary, 3330 Hospital Dr NW, Calgary, Alberta T2N 4N1, Canada; Calgary Laboratory Services, 3535 Research Rd NW, Calgary, Alberta T2L 2K8, Canada; Departments of Family Medicine and Community Health Services, Cumming School of Medicine, University of Calgary, 3330 Hospital Dr NW, Calgary, Alberta, Canada T2N 4N1. Electronic address:
    Objectives: Serum iron is an important clinical test to help identify cases of iron deficiency or overload. Fluctuations caused by diurnal variation and diet are thought to influence test results, which may affect clinical patient management. We examined the impact of these preanalytical factors on iron concentrations in a large community-based cohort. Read More

    Causes, consequences and management of sample hemolysis in the clinical laboratory.
    Clin Biochem 2017 Sep 22. Epub 2017 Sep 22.
    Department of Laboratory Medicine, Ziekenhuis Netwerk Antwerpen, Antwerp, Belgium.
    Preanalytical hemolysis of blood samples is a common problem in medical practice, especially in emergency departments. Several potential influences on sample hemolysis have been investigated, including sampling techniques, centrifugation and sample transport. In particular, the use of intravenous catheters and the vacuum sampling technique have often been demonstrated to provoke hemolysis. Read More

    Dysregulation of serum NADPH oxidase1 and ferritin levels provides insights into diagnosis of Parkinson's disease.
    Clin Biochem 2017 Sep 20. Epub 2017 Sep 20.
    Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:
    Objective: Parkinson's disease (PD) is a common neurodegenerative disease. Oxidative stress is considered as a key modulator in the development of PD. This study aimed to investigate associations between serum NOX1 (NADPH oxidase1), ferritin, selenium (Se), and uric acid (UA) levels and clinical parameters in patients with PD. Read More

    IgE monoclonal gammopathy: A case report and literature review.
    Clin Biochem 2017 Sep 21. Epub 2017 Sep 21.
    Biology Department, Hôpital d'Instruction des Armées Percy, Percy Military Hospital, 101 Avenue Henri Barbusse, 92140 Clamart, France.
    Immunoglobulin E (IgE) gammopathy is a rare disorder, accounting for just 0.1% of all patients with multiple myeloma (MM). Herein, we report a case of IgE monoclonal gammopathy without any biological and clinical symptoms, and we review 63 published cases in the literature. Read More

    Method performance and clinical workflow outcomes associated with meconium and umbilical cord toxicology testing.
    Clin Biochem 2017 Sep 21. Epub 2017 Sep 21.
    Department of Pathology, Ohio State University Wexner Medical Center, Columbus, OH, United States. Electronic address:
    Objective: Neonatal abstinence syndrome (NAS) is a rising concern with unknown long-term effects. It is apparent that higher cost of care, impact on the community and reduced quality of life are associated with similar etiologies (e.g. Read More

    Removal of uremic retention products by hemodialysis is coupled with indiscriminate loss of vital metabolites.
    Clin Biochem 2017 Sep 18. Epub 2017 Sep 18.
    Division of Nephrology and Hypertension, School of Medicine, University of California Irvine, Irvine, CA 92897, USA. Electronic address:
    Background: Although dialysis ameliorates uremia and fluid and electrolytes disorders, annual mortality rate remains high in dialysis population reflecting its shortcoming in replacing renal function. Unlike the normal kidney, dialysis causes dramatic shifts in volume and composition of body fluids and indiscriminate removal of vital solutes. Present study was undertaken to determine the impact of hemodialysis on plasma metabolites in end-stage renal disease (ESRD) patients. Read More

    Combining Nordtest method and bootstrap resampling for measurement uncertainty estimation of hematology analytes in a medical laboratory.
    Clin Biochem 2017 Sep 18. Epub 2017 Sep 18.
    Center of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, China. Electronic address:
    Background: Measurement uncertainty (MU) is a metrological concept, which can be used for objectively estimating the quality of test results in medical laboratories. The Nordtest guide recommends an approach that uses both internal quality control (IQC) and external quality assessment (EQA) data to evaluate the MU. Bootstrap resampling is employed to simulate the unknown distribution based on the mathematical statistics method using an existing small sample of data, where the aim is to transform the small sample into a large sample. Read More

    Sensitive and selective quantification of total and free itraconazole and hydroxyitraconazole in human plasma using ultra-performance liquid chromatography coupled to tandem mass spectrometry.
    Clin Biochem 2017 Sep 18. Epub 2017 Sep 18.
    Department of Clinical Pharmacy, Oita University Hospital, Hasama-machi, Oita, Japan.
    Objectives: Protein-free (unbound) drug concentrations have been reported to be better biomarker of pharmacodynamics compared with total drug concentrations. In this study, we developed and validated an assay for the quantification of total and free itraconazole and hydroxyitraconazole, a main metabolite with antifungal activity, in human plasma using ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS).

    Design & Methods: Plasma sample was ultra-filtrated for the measurement of free itraconazole and hydroxyitraconazole concentrations. Read More

    Assessing precision, bias and sigma-metrics of 53 measurands of the Alinity ci system.
    Clin Biochem 2017 Sep 14. Epub 2017 Sep 14.
    Abbott, Abbott Park, IL, USA.
    Assay performance is dependent on the accuracy and precision of a given method. These attributes can be combined into an analytical Sigma-metric, providing a simple value for laboratorians to use in evaluating a test method's capability to meet its analytical quality requirements. Sigma-metrics were determined for 37 clinical chemistry assays, 13 immunoassays, and 3 ICT methods on the Alinity ci system. Read More

    Analytical sensitivity and diagnostic performance of serum protein electrophoresis on the HYDRAGEL 30 PROTEIN(E) β1-β2 Sebia Hydrasys system.
    Clin Biochem 2017 Sep 12. Epub 2017 Sep 12.
    Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada; DynaLIFE(MEDICAL LABS), Edmonton, Alberta, Canada. Electronic address:
    Background: Serum protein electrophoresis (SPE) and immunofixation electrophoresis (IFE) are used in the diagnosis and monitoring of plasma cell dyscrasias. IFE is considered the most sensitive method for the detection of monoclonal proteins (M-proteins), but it is not quantitative. The goal of this study was to establish the analytical sensitivity and diagnostic performance of SPE on the Sebia Hydrasys using HYDRAGEL 30 PROTEIN(E) β1-β2. Read More

    Sex-specific 99th percentiles derived from the AACC Universal Sample Bank for the Roche Gen 5 cTnT assay: Comorbidities and statistical methods influence derivation of reference limits.
    Clin Biochem 2017 Sep 13. Epub 2017 Sep 13.
    Department of Laboratory Medicine and Pathology, Hennepin County Medical Center, University of Minnesota, Minneapolis, MN, USA. Electronic address:
    Objectives: Our purpose was to determine a) overall and sex-specific 99th percentile upper reference limits (URL) and b) influences of statistical methods and comorbidities on the URLs.

    Methods: Heparin plasma from 838 normal subjects (423 men, 415 women) were obtained from the AACC (Universal Sample Bank). The cobas e602 measured cTnT (Roche Gen 5 assay); limit of detection (LoD), 3ng/L. Read More

    IgG subclasses quantitation: Analytical performance of The Binding Site SPAPLUS® human assay and comparison with Siemens BNII® assay.
    Clin Biochem 2017 Sep 11. Epub 2017 Sep 11.
    Service of Clinical Pathology, La Fe University Hospital, Av Fernando Abril Martorell 106, 46026, Valencia, Spain.
    Objectives: Accurate evaluation of analyzers is highly recommended before these devices are broadly introduced for routine testing. Concerning quantification of IgG subclasses (IgGSc), standardization has not yet been reached and thus different assays might lead to different results. Here we report the analytical performances of The Binding Site (TBS) SPAPLUS® human IgGSc assay and the concordance with the Siemens BNII® human IgGSc assay. Read More

    Analytical verification and method comparison of the ADVIA Centaur® Intact Parathyroid Hormone assay.
    Clin Biochem 2017 Sep 11. Epub 2017 Sep 11.
    Department of Biochemistry and Molecular Genetics & Core Lab (CDB), Hospital Clínic, IDIBAPS, Barcelona, Catalonia, Spain. Electronic address:
    Objectives: This study is the first verification of the novel iPTH Siemens ADVIA Centaur® Intact Parathyroid Hormone (iPTHm) chemiluminescence immunoassay based on monoclonal antibodies. We also compared the iPTH results obtained using this assay with the previous ADVIA Centaur® Parathyroid Hormone assay (iPTHp) based on polyclonal antibodies.

    Design And Methods: The analytical performance study of iPTHm assay included LoD, LoQ, intra- and inter-assay reproducibility, and linearity. Read More

    Biochemical differences in the mass and activity tests of lipoprotein-associated phospholipase A2 explain the discordance in results between the two assay methods.
    Clin Biochem 2017 Sep 9. Epub 2017 Sep 9.
    Diazyme Laboratories, Inc., 12889 Gregg Ct., Poway, CA 92064, United States.
    Objectives: There are two platforms for the detection of Lp-PLA2 in sera or plasmas: by its enzymatic activity (PLAC® activity test) and by its mass concentration (PLAC® mass test). It has been long recognized that these two platforms are not correlated well. The underlying cause for this is therefore investigated by the biochemical characterization of the two PLAC tests. Read More

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