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    6239 results match your criteria Clinical Biochemistry [Journal]

    1 OF 125

    Establishment of reference intervals for the salivary cortisol circadian cycle, by electrochemiluminescence (ECLIA), in healthy adults.
    Clin Biochem 2018 Feb 19. Epub 2018 Feb 19.
    Department of Medical Biology, CIUSSS de l'Estrie - CHUS, Fleurimont, Canada. Electronic address:
    Objectives: To determine salivary cortisol reference intervals in a healthy adult population, at 6 different time points during a 24-hour (h) period.

    Methods: In a prospective study, salivary cortisol concentrations were measured upon waking, one-hour post-waking and at specific times of the day: at 12 h00, 16 h00, 20 h00 and midnight. Samples were analyzed by the first and second-generation electrochemiluminescence assays (ECLIA) from Roche Cobas Cortisol®. Read More

    FGF 23, PTH and vitamin D status in end stage renal disease patients affected by VDR FokI and BsmI variants.
    Clin Biochem 2018 Feb 16. Epub 2018 Feb 16.
    Immuno-Rheumatology Research Laboratory, Rheumatology Department, La Rabta Hospital, University of Tunis-El Manar, Tunis, Tunisia; Laboratory of Clinical Biochemistry and Hormonology, Pasteur Institute of Tunis, Tunis El Manar University, Tunisia.
    Objectives: The aim of this study was to evaluate the association between two VDR SNPs FokI and BsmI and mineral status in ESRD patients.

    Design And Methods: Our case-control study included 100 patients with chronic renal failure in ESRD and 149 healthy subjects. We measured the serum Vitamin D levels and the serum intact PTH level by Electrochemiluminescence Technology (cobas E411 analyzer). Read More

    The effect of time of day testing and utility of 30 and 60 minute cortisol values in the 250 mcg ACTH stimulation test.
    Clin Biochem 2018 Feb 16. Epub 2018 Feb 16.
    Division of Endocrinology, Department of Medicine, Dalhousie University, Halifax, NS, Canada. Electronic address:
    Background: Despite widespread use of the 250-mcg Cosyntropin test (ACTH test) for the diagnosis of adrenal insufficiency (AI), the effect of time of day and the utility of performing both 30- and 60-min serum cortisol values remains unclear.

    Methods: We conducted a retrospective cohort study of all ACTH testing at the Halifax Neuropituitary Program, Nova Scotia, Canada between January 2006 and April 2016. Data were collected on age, gender, medication history, serum cortisol levels at 0, 30 and 60 min after ACTH administration, as well as time of and indication for testing. Read More

    The role of adiponectin and adipolin as anti-inflammatory adipokines in the formation of macrophage foam cells and their association with cardiovascular diseases.
    Clin Biochem 2018 Feb 13. Epub 2018 Feb 13.
    Department of Biochemistry, Institute Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
    Obesity is one of the major public health concerns that is closely associated with obesity-related disorders such as type 2 diabetes mellitus (T2DM), hypertension, and atherosclerosis. Atherosclerosis is a chronic disease characterized by excess cholesterol deposition in the arterial intima and the formation of foam cells. Adipocytokines or adipokines are secreted by the adipose tissue as endocrine glands; adiponectin and adipolin are among these adipokines that are associated with obese and insulin-resistant phenotypes. Read More

    Diurnal variation in admission troponin concentrations in patients with chest pain in the emergency department.
    Clin Biochem 2018 Feb 13. Epub 2018 Feb 13.
    Department of Medicine, Karolinska Institutet, Solna, SE-171 71 Stockholm, Sweden; Functional area of Emergency Medicine, Karolinska University Hospital, Huddinge, SE-141 86, Stockholm, Sweden. Electronic address:
    Background: Recent studies have suggested that there may be large diurnal variation in cardiac troponin T (cTnT) concentrations measured with a high sensitive assay.

    Objective: To investigate if clinically relevant diurnal variation in cTnT concentrations is present in patients with chest pain in the emergency department (ED).

    Methods: We included all patients with chest pain, but no myocardial infarction (MI), and no other acute condition that may affect troponin concentrations in the ED at Karolinska University Hospital, Stockholm, Sweden, 2011-2014. Read More

    Serum ARCHITECT PIVKA-II reference interval in healthy Chinese adults: Sub-analysis from a prospective multicenter study.
    Clin Biochem 2018 Feb 12. Epub 2018 Feb 12.
    Department of Laboratory Medicine, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710000, China. Electronic address:
    Background: Protein induced by vitamin K absence or antagonist-II (PIVKA-II) has been widely used as a biomarker for liver cancer diagnosis in Japan for decades. However, the reference intervals for serum ARCHITECT PIVKA-II have not been established in the Chinese population. Thus, this study aimed to measure serum PIVKA-II levels in healthy Chinese subjects. Read More

    Serum and dietary zinc and copper in Iranian girls.
    Clin Biochem 2018 Feb 10. Epub 2018 Feb 10.
    Metabolic Syndrome Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:
    Objective: Girls with micronutrient deficiencies may have impaired growth and development, and furthermore this may also impact on their childbearing. We have investigated the relationship between serum zinc and copper concentrations, dietary zinc and copper intake and anthropometric and demographic parameters, and cardiovascular risk factors, in 408 girls living in northeastern Iran.

    Methods: A total of 408 healthy girls, aged 12-18 years old, were included in our study. Read More

    Paper spray mass spectrometry for the direct, semi-quantitative measurement of fentanyl and norfentanyl in complex matrices.
    Clin Biochem 2018 Feb 9. Epub 2018 Feb 9.
    Applied Environmental Research Laboratories (AERL), Department of Chemistry, Vancouver Island University, Nanaimo, BC, Canada; Department of Chemistry, University of Victoria, Victoria, BC, Canada; Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada; Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA. Electronic address:
    Background: Fentanyl is a potent, synthetic opioid at the centre of an international health crisis that has seen thousands of fatal overdoses. Most analytical methods focus on the detection of fentanyl in blood and/or urine (i.e. Read More

    A novel NMR-based assay to measure circulating concentrations of branched-chain amino acids: Elevation in subjects with type 2 diabetes mellitus and association with carotid intima media thickness.
    Clin Biochem 2018 Feb 9. Epub 2018 Feb 9.
    Department of Endocrinology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. Electronic address:
    Objectives: Plasma branched-chain amino acid (BCAA) levels, measured on nuclear magnetic resonance (NMR) metabolomics research platforms or by mass spectrometry, have been shown to be associated with type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD). We developed a new test for quantification of BCAA on a clinical NMR analyzer and used this test to determine the clinical correlates of BCAA in 2 independent cohorts.

    Design And Methods: The performance of the NMR-based BCAA assay was evaluated. Read More

    The role of external quality assessment in the verification of in vitro medical diagnostics in the traceability era.
    Clin Biochem 2018 Feb 8. Epub 2018 Feb 8.
    Research Centre for Metrological Traceability in Laboratory Medicine (CIRME), University of Milan, Milan, Italy.
    Once an in-vitro diagnostic (IVD) measuring system has been marketed and introduced into daily practice, the possible sources of degradation of its performance are numerous. It is therefore essential to put in place a continuous post-market surveillance of the quality of performance of the IVD system and of the laboratories that perform measurements in clinical setting. The participation to external quality assessment (EQA) schemes that meet specific metrological criteria is central to the evaluation of performance of clinical laboratories in terms of standardization and clinical suitability of their measurements. Read More

    Analytical and clinical validation of an LC-MS/MS method to measure thiopurine S-methyltransferase activity by quantifying d3-6-MMP.
    Clin Biochem 2018 Feb 6. Epub 2018 Feb 6.
    Specialist Chemistry, Canterbury Health Laboratories, Christchurch, New Zealand. Electronic address:
    Background: Identification of patients with thiopurine S-methyltransferase (TPMT) deficiency prior to thiopurine drug therapy has become routine clinical practice worldwide. To measure TPMT activity, traditional radiochemical assays have been replaced by chromatographic methods.

    Method: Inspired by the increasing number of isotope labelled sources that may be of benefit for the TPMT assay, a new LC-MS/MS method for TPMT activity was developed and validated. Read More

    Effects of co-existing autoimmune diseases on serum lipids and lipoprotein subclasses profile in paediatric patients with type 1 diabetes mellitus.
    Clin Biochem 2018 Feb 2. Epub 2018 Feb 2.
    Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
    Objective: Paediatric patients with type 1 diabetes mellitus (T1DM) frequently develop other autoimmune disorders; most commonly autoimmune thyroiditis (ATD) and celiac disease (CD). In this study we evaluated whether co-existing autoimmune diseases had significant impact on lipid and lipoprotein subclasses, as known cardiovascular risk factors in T1DM.

    Design And Methods: Study included 201 subjects with T1DM (14. Read More

    Measurement uncertainty: Friend or foe?
    Clin Biochem 2018 Feb 2. Epub 2018 Feb 2.
    Research Centre for Metrological Traceability in Laboratory Medicine (CIRME), University of Milan, Milano, Italy.
    The definition and enforcement of a reference measurement system, based on the implementation of metrological traceability of patients' results to higher order reference methods and materials, together with a clinically acceptable level of measurement uncertainty, are fundamental requirements to produce accurate and equivalent laboratory results. The uncertainty associated with each step of the traceability chain should be governed to obtain a final combined uncertainty on clinical samples fulfilling the requested performance specifications. It is important that end-users (i. Read More

    Serum uric acid in Friedreich Ataxia.
    Clin Biochem 2018 Feb 2. Epub 2018 Feb 2.
    Department of Neurosciences and Neurorehabilitation, IRCCS Bambino Gesù Children Hospital, Rome, Italy.
    Serum uric acid (UA) is a circulating antioxidant whose levels are typically lower in patients with idiopathic neurodegenerative diseases than healthy controls, reflecting a higher oxidative stress. Here we provided the first assessment of serum UA in Friedreich Ataxia (FRDA), an inherited neurodegenerative disorder, aimed at exploring novel disease biomarkers. Serum UA was measured in 19 FRDA patients and compared to 26 healthy controls (CTL). Read More

    Are laboratory tests always needed? Frequency and causes of laboratory overuse in a hospital setting.
    Clin Biochem 2018 Feb 2. Epub 2018 Feb 2.
    Department of Laboratory Medicine, Paracelsus Medical University, Salzburg, Austria.
    Background: Inappropriate utilization of laboratory resources is an increasing concern especially in high-throughput facilities. Until now, no reliable information has been published addressing to which extent laboratory results are actually used for clinical decision-making. Therefore, we aimed to close this gap using a novel retrospective approach including a survey of clinicians and nurses. Read More

    A novel method for quantitation of acylglycines in human dried blood spots by UPLC-tandem mass spectrometry.
    Clin Biochem 2018 Feb 2. Epub 2018 Feb 2.
    Newborn Screening Ontario, Children's Hospital of Eastern Ontario, 415 Smyth Road, Ottawa, Ontario, Canada; Children's Hospital of Eastern Ontario Research Institute, Ottawa, Canada; University of Ottawa, Department of Pediatrics, 451 Smyth Road, Ottawa, Ontario, Canada; University of Ottawa, Department of Pathology and Laboratory Medicine, 451 Smyth Road, Ottawa, Ontario, Canada.
    Background: Several acylcarnitines used as primary markers on dried blood filter papers (DBS) for newborn screening lack specificity and contribute to a higher false positive rate. The analysis of urine acylglycines is useful in the diagnosis of inborn errors of metabolism (IEM) including medium chain acyl-CoA dehydrogenase deficiency (MCADD), isovaleric acidemia, and beta-ketothiolase deficiency (BKTD). Currently, no method for analyzing acylglycines from DBS has been published. Read More

    What information on measurement uncertainty should be communicated to clinicians, and how?
    Clin Biochem 2018 Feb 2. Epub 2018 Feb 2.
    Department of Laboratory Medicine, University-Hospital of Padova, Via Giustiniani 2, 35128 Padova, Italy.
    The communication of laboratory results to physicians and the quality of reports represent fundamental requirements of the post-analytical phase in order to assure the right interpretation and utilization of laboratory information. Accordingly, the International Standard for clinical laboratories accreditation (ISO 15189) requires that "laboratory reports shall include the information necessary for the interpretation of the examination results". Measurement uncertainty (MU) is an inherent property of any quantitative measurement result which express the lack of knowledge of the true value and quantify the uncertainty of a result, incorporating the factors known to influence it. Read More

    Determining the utility of creatinine delta checks: A large retrospective analysis.
    Clin Biochem 2018 Feb 1. Epub 2018 Feb 1.
    Department of Laboratory Medicine and Pathology, University of Minnesota, 420 Delaware St SE, MMC 609, Minneapolis, MN 55455, United States. Electronic address:
    Introduction: Delta checks are a long-standing practice for identifying errors in the laboratory. However, with the decrease in errors due to laboratory automation, their utility is unclear. The objective of this retrospective analysis was to determine whether establishment of a creatinine delta check would be an effective means for capturing true laboratory error. Read More

    Assessment of biotin interference with qualitative point-of-care hCG test devices.
    Clin Biochem 2018 Jan 29. Epub 2018 Jan 29.
    Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH, United States; The Geisel School of Medicine at Dartmouth, Hanover, NH, United States. Electronic address:
    Biotin is commonly used as a dietary supplement for the claimed benefits of promoting healthy hair and nail growth and is available without a prescription at doses up to 10mg/capsule. Biotin-mediated interference in immunoassay testing is an emerging issue for clinical laboratories and previous studies have indicated that biotin at regularly encountered doses may interfere with these assays. In this study, we evaluated the effect of supplemental biotin on seven POC urine hCG test devices using purified biotin and urine collected from four volunteers consuming 10mg biotin/day. Read More

    Establishing reference intervals for ALT, AST, UR, Cr, and UA in apparently healthy Chinese adolescents.
    Clin Biochem 2018 Jan 30. Epub 2018 Jan 30.
    Department of Laboratory Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan, PR China. Electronic address:
    Background: The current child-specific reference intervals (RIs) are inadequate or even unavailable for many analyses in China. Many of the RIs used in Chinese laboratories were derived from Chinese adult standards or from foreign studies. The aim of this study was to establish specific RIs for alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea (UR), creatinine (Cr) and uric acid (UA) for apparently healthy Chinese adolescents. Read More

    Diagnostic implications of enumerating and reporting beta fraction(s) for the detection of beta-migrating monoclonal immunoglobulins in serum protein electrophoresis.
    Clin Biochem 2018 Jan 31. Epub 2018 Jan 31.
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada.
    Background: Modern serum protein electrophoresis (SPE) resolves serum proteins in 6 major fractions, splitting beta (B) into beta1 (B1) and beta2 (B2). Beta-migrating monoclonal immunoglobulins (B-MC) commonly integrate into normal-looking peak shape(s) but may increase the fraction value, forming the basis for reflex testing. The objectives of this study were (1) to ascertain the value of beta fraction(s) reporting, and (2) to compare the diagnostic performance between different beta-flagging approaches, particularly ↑B versus ↑B1 and/or ↑B2. Read More

    Performance evaluation of the new measurement channels on the automated Sysmex XN-9000 hematology analyzer.
    Clin Biochem 2018 Feb 2. Epub 2018 Feb 2.
    Department of Molecular Medicine and Haematology, National Health Laboratory Service, Johannesburg, South Africa.
    Background: The automated Sysmex XN-9000 hematology system has been designed to meet the throughput and efficiency requirements of high volume laboratories with predominantly abnormal samples. New measurement channels have been introduced namely the white cell nucleated (WNR), white cell differential (WDF), white cell precursor (WPC) and fluorescent platelet (PLT-F) channels.

    Methods: The performance of the new measurement channels was evaluated with regards to precision, accuracy, linearity, carryover, throughput and stability. Read More

    The predictive value of NT-proBNP and hs-TnT for risk of death in cardiac surgical patients.
    Clin Biochem 2018 Jan 31. Epub 2018 Jan 31.
    Division of Medicine, Akershus University Hospital, Lørenskog, Norway; Center for Heart Failure Research, Institute of Clinical Medicine, University of Oslo, Oslo, Norway. Electronic address:
    Background: European System for Cardiac Operative Risk Evaluation II (EuroSCORE II) is used for risk stratification before cardiac surgery, but whether N-terminal pro-B-type natriuretic peptide (NT-proBNP) and high-sensitivity troponin T (hs-TnT) may add prognostic information to EuroSCORE II is not known.

    Methods: Preoperative (n=640) and postoperative (n=629) blood samples were available from cardiac surgical patients with 961-day follow-up (FINNAKI Heart study; cohort #1). The accuracy of a parsimonious risk model with NT-proBNP measurements was also tested in 90 patients with respiratory failure after cardiac surgery (FINNALI study; cohort #2). Read More

    Trajectories of eGFR decline over a four year period in an Indigenous Australian population at high risk of CKD-the eGFR follow up study.
    Clin Biochem 2018 Feb 2. Epub 2018 Feb 2.
    Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia; Department of Medicine, Royal Darwin Hospital, Darwin, North Territory, Australia.
    Being able to estimate kidney decline accurately is particularly important in Indigenous Australians, a population at increased risk of developing chronic kidney disease and end stage kidney disease. The aim of this analysis was to explore the trend of decline in estimated glomerular filtration rate (eGFR) over a four year period using multiple local creatinine measures, compared with estimates derived using centrally-measured enzymatic creatinine and with estimates derived using only two local measures.

    Method: The eGFR study comprised a cohort of over 600 Aboriginal Australian participants recruited from over twenty sites in urban, regional and remote Australia across five strata of health, diabetes and kidney function. Read More

    iTRAQ analysis of urinary proteins: Potential use of gelsolin and osteopontin to distinguish benign thyroid goiter from papillary thyroid carcinoma.
    Clin Biochem 2018 Jan 23. Epub 2018 Jan 23.
    University of Malaya Centre for Proteomics Research, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address:
    Background: Benign thyroid goiter (BTG) and papillary thyroid carcinoma (PTC) are often interchangeably misdiagnosed.

    Methods: Pooled urine samples of patients with BTG (n=10), patients with PTC (n=9) and healthy controls (n=10) were subjected to iTRAQ analysis and immunoblotting.

    Results: The ITRAQ analysis of the urine samples detected 646 proteins, 18 of which showed significant altered levels (p<0. Read More

    Random uncertainty of photometric determination of hemolysis index on the Abbott Architect c16000 platform.
    Clin Biochem 2018 Jan 16. Epub 2018 Jan 16.
    Clinical Pathology Unit, 'Luigi Sacco' University Hospital, Milan, Italy.
    Background: Automatic photometric determination of the hemolysis index (HI) on serum and plasma samples is central to detect potential interferences of in vitro hemolysis on laboratory tests. When HI is above an established cut-off for interference, results may suffer from a significant bias and undermine clinical reliability of the test. Despite its undeniable importance for patient safety, the analytical performance of HI estimation is not usually checked in laboratories. Read More

    Analytical and pre-analytical performance characteristics of a novel cartridge-type blood gas analyzer for point-of-care and laboratory testing.
    Clin Biochem 2018 Jan 12. Epub 2018 Jan 12.
    Department of Laboratory Medicine, Ghent University Hospital, Ghent, Belgium.
    Background: Point-of-care blood gas test results may benefit therapeutic decision making by their immediate impact on patient care. We evaluated the (pre-)analytical performance of a novel cartridge-type blood gas analyzer, the GEM Premier 5000 (Werfen), for the determination of pH, partial carbon dioxide pressure (pCO), partial oxygen pressure (pO), sodium (Na), potassium (K), chloride (Cl), ionized calcium (Ca), glucose, lactate, and total hemoglobin (tHb).

    Methods: Total imprecision was estimated according to the CLSI EP5-A2 protocol. Read More

    Establishment of trimester-specific reference range for thyroid hormones during pregnancy.
    Clin Biochem 2018 Jan 11. Epub 2018 Jan 11.
    Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:
    Objective: Physiological gestational changes are associated with alterations in thyroid function which require different biochemical interpretation from that of non-pregnant women and necessitate established pregnancy-specific reference ranges. We aimed to identify the trimester-specific ranges of thyroid markers in a healthy population of pregnant Iranian women.

    Methods: In this self-sequential study, data were extracted from The Tehran Thyroid and Pregnancy Study; a total of 314 women were tested during the 1st, 2nd and 3rd trimesters for serum levels of thyrotropin (TSH), thyroxine (T4), free thyroxine index (FT4I) and thyroid peroxidase antibody (TPOAb). Read More

    Combined creatinine-cystatin C CKD-EPI equation significantly underestimates measured glomerular filtration rate in people with type 2 diabetes mellitus.
    Clin Biochem 2018 Jan 10. Epub 2018 Jan 10.
    Postgraduate Program in Medical Sciences: Endocrinology, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil; Endocrine Unit, Hospital de Clínicas de Porto Alegre, Brazil. Electronic address:
    Aim: To evaluate the accuracy of creatinine and cystatin C (cysC) equations to estimate glomerular filtration rate (GFR) in type 2 diabetes mellitus (DM) patients and healthy adults.

    Methods: Case-control study including 84 patients with type 2 DM and 100 healthy adults with measured GFR (mGFR)≥60mL/min/1.73m. Read More

    Simultaneous determination of voriconazole, posaconazole, itraconazole and hydroxy-itraconazole in human plasma using LCMS/MS.
    Clin Biochem 2018 Jan 9. Epub 2018 Jan 9.
    Clinical Biochemistry Unit, Alfred Pathology Service, Melbourne, Australia; Monash University, Melbourne, Australia.
    Introduction: Invasive fungal infections are an increasing cause of mortality and morbidity in high risk patient populations such as those on immunosuppressive therapy. Triazole antifungals are recommended for the prevention and treatment of such infections. The aim of this study was to develop and validate a simple, sensitive and robust LCMS/MS method for the simultaneous analysis in human plasma of three frequently used antifungal drugs: voriconazole, posaconazole, and itraconazole. Read More

    CLSI-based transference and verification of CALIPER pediatric reference intervals for 29 Ortho VITROS 5600 chemistry assays.
    Clin Biochem 2018 Jan 4. Epub 2018 Jan 4.
    Department of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada; CALIPER Program, Pediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada. Electronic address:
    Background: Evidence-based reference intervals (RIs) are essential to accurately interpret pediatric laboratory test results. To fill gaps in pediatric RIs, the Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) project developed an age- and sex-specific pediatric RI database based on healthy pediatric subjects. Originally established for Abbott ARCHITECT assays, CALIPER RIs were transferred to assays on Beckman, Roche, Siemens, and Ortho analytical platforms. Read More

    Simple and efficient screening of patients with Fabry disease with high resolution melting.
    Clin Biochem 2018 Jan 4. Epub 2018 Jan 4.
    Post-Graduation Program on Genetics and Molecular Biology, UFRGS, Porto Alegre, RS 91501-970, Brazil; Gene Therapy Center, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-903, Brazil; Department of Genetics, UFRGS, Porto Alegre, RS 91501-970, Brazil. Electronic address:
    Background: Fabry disease (FD [MIM: 301500]) is a disorder caused by mutations in the alpha-galactosidase gene (GLA), which presents great allelic heterogeneity. The development of fast screening methods may reduce costs and length of diagnosis, being particularly important for screening programs of high-risk female patients. Therefore, the purpose of this study was to develop a pre-sequencing genetic screening method based on high resolution melting (HRM) analysis. Read More

    Comparing analytical outliers and the percent of emergency department patients with results above the 99th percentile upper reference limit for 2 conventional and one high sensitivity troponin assay.
    Clin Biochem 2018 Jan 2. Epub 2018 Jan 2.
    Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First St SW, Rochester, MN 55905, United States; Department of Cardiology, Mayo Clinic, 200 First St SW, Rochester, MN 55905, United States.
    Objectives: We compared rates of analytical outliers, and percent of emergency department (ED) patients with cardiac troponin (cTn) values above the 99th percentile upper reference limit (URL), for two conventional and one high sensitivity cTn assay.

    Methods: We measured 3008 samples from 1931 ED patients by Roche e411 4th generation Troponin T (cTnT); and Abbott STAT Troponin I (cTnI) and high sensitivity troponin I (hscTnI) on an Architect i2000. Within 24h of initial measurement, samples were aliquoted, re-centrifuged, and repeated in duplicate by all methods. Read More

    Association of vascular indices with novel circulating biomarkers as prognostic factors for cardiovascular complications in patients with type 2 diabetes mellitus.
    Clin Biochem 2017 Dec 27. Epub 2017 Dec 27.
    2nd Department of Cardiology, Medical School, University of Ioannina, Ioannina 45110, Greece; Michaelidion Cardiac Center, Medical School, University of Ioannina, Ioannina 45110, Greece.
    Background: The pathophysiology of atherosclerosis in type 2 diabetes mellitus (T2DM) is multifactorial. The association of vascular indices with circulating biomarkers of inflammation and insulin resistance and their role in the long-term cardiovascular prognosis in T2DM patients were currently investigated.

    Patients And Methods: Patients with T2DM and poor glycemic control without known cardiovascular diseases (n=119) at baseline were enrolled and followed for about 9years. Read More

    Comparative evaluation of neonatal bilirubin using Radiometer whole blood co-oximetry and plasma bilirubin methods from Roche Diagnostics and Ortho Clinical Diagnostics.
    Clin Biochem 2017 Dec 20. Epub 2017 Dec 20.
    Department of Pathology & Laboratory Medicine, Division of Clinical Biochemistry, Saskatoon Health Region, Saskatoon, Saskatchewan, Canada. Electronic address:
    Background: Clinically significant variation has been reported within and between plasma and whole blood total bilirubin methods used to identify neonates for whom clinical intervention for hyperbilirubinemia may be required.

    Objective: To evaluate total bilirubin measurements between the Radiometer whole blood co-oximeter and plasma bilirubin methods from Roche Diagnostics and Ortho Clinical Diagnostics using neonatal specimens.

    Methods: Total bilirubin levels were analyzed by whole blood co-oximetry (Radiometer® ABL90). Read More

    Validation of the Friedewald formula for the estimation of low density lipoprotein cholesterol in a sub-Saharan African population.
    Clin Biochem 2017 Dec 20. Epub 2017 Dec 20.
    South African Medical Research Council, University of Cape Town, Cape Town, South Africa.
    Background: Low density lipoprotein cholesterol (LDL-C) levels are used to estimate cardiovascular disease (CVD) risk and to guide prescriptions. To circumvent the challenges of direct LDL-C measurement, guidelines recommend the use of Friedewald formula derived LDL-C levels. Despite reported limitations of this formula, its validity in sub-Saharan Africans has not been adequately investigated. Read More

    Elevation of serum pyruvate kinase M2 (PKM2) in IBD and its relationship to IBD indices.
    Clin Biochem 2017 Dec 19. Epub 2017 Dec 19.
    Drug Discovery and Development Research Group, College of Pharmacy and Nutrition, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan, Canada. Electronic address:
    Objectives: Endoscopy remains the gold standard to diagnose and evaluate inflammatory bowel disease (IBD) activity. Current biomarkers or their combinations cannot adequately predict IBD risk, diagnosis, progression or relapse, and response to therapy. Pyruvate kinase M2 (PKM2) is emerging as a significant mediator of the inflammatory process. Read More

    How to best use high-sensitivity cardiac troponin in patients with suspected myocardial infarction.
    Clin Biochem 2017 Dec 16. Epub 2017 Dec 16.
    Cardiovascular Research Institute Basel (CRIB), Department of Cardiology, University Hospital Basel, University of Basel, Basel, Switzerland. Electronic address:
    High-sensitivity cardiac troponin (hs-cTn) assays have been used clinically by thousands of physicians in many countries throughout the world since their clinical introduction seven years ago. In the early diagnosis of myocardial infarction (MI), beyond doubt the most important indication of hs-cTn assays, these simple, inexpensive and highly reproducible tools complement detailed clinical assessment including chest pain characteristics and the electrocardiogram. Hs-cTn assays for the first time allowed the precise quantification of cardiomyocyte injury around the 99th percentile and thereby substantially increased the accuracy of MI detection from blood obtained at presentation to the emergency department (ED). Read More

    Two Thyroid Stimulating Hormone assays correlated in clinical practice show disagreement in subclinical hypothyroidism patients.
    Clin Biochem 2017 Dec 15. Epub 2017 Dec 15.
    Molecular Innovation and Biotechnology Laboratory, Medical Postgraduation Division, Universidade Nove de Julho (Uninove), São Paulo, SP, Brazil. Electronic address:
    Thyroid Stimulating Hormone (TSH) levels are related to the pituitary gland's ability to detect thyroid hormone concentration. Many studies have analyzed the correlation between TSH and T4, demonstrating a complex system correlation. This complex system may vary among different TSH levels and patients. Read More

    Triglycerides and glycated hemoglobin for screening insulin resistance in obese patients.
    Clin Biochem 2017 Dec 7. Epub 2017 Dec 7.
    PHYMEDEXP, CNRS, INSERM, Univ Montpellier, Department of Nutrition and Diabetes, CHU Montpellier, Montpellier, France.
    Objective: Assessment of insulin resistance (IR) is essential in non-diabetic patients with obesity. Thus study aims to identify the best determinants of IR and to propose an original approach for routine assessment of IR in obesity.

    Design And Patients: All adult with obesity defined by a body mass index ≥30kg/m, evaluated in the Nutrition Department between January 2010 and January 2015 were included in this cross-sectional study. Read More

    Hyperbaric oxygen ameliorated the lesion scope and nerve function in acute spinal cord injury patients: A retrospective study.
    Clin Biochem 2017 Dec 5. Epub 2017 Dec 5.
    Department of Hyperbaric Oxygen, Beijing Chaoyang Hospital Affiliated to Capital University of Medical Science, China. Electronic address:
    Objective: This is a retrospective study to assess the therapeutic effect of hyperbaric oxygen (HBO) in early treatment of acute spinal cord injury (SCI) using magnetic resonance imaging (MRI) and electrophysiology in diagnosing.

    Methods: Forty acute SCI patients from Sun Yat-Sen Memorial Hospital who were assigned into HBO treatment were included during August 2013 to October 2014.The patients with adverse reactions or contraindications for HBO were assigned as controls. Read More

    Impact of long distance rowing on biological health: A pilot study.
    Clin Biochem 2018 Feb 2;52:142-147. Epub 2017 Dec 2.
    Division of Laboratory Medicine, Department of Genetics and Laboratory Medicine, Geneva University Hospitals, 4 rue Gabrielle-Perret-Gentil, 1205 Geneva, Switzerland; Department of Human Protein Sciences, Faculty of Medicine, University of Geneva, 1 rue Michel Servet, 1206 Geneva, Switzerland.
    Objectives: To determine the impact of long distance rowing (160km, nonstop) on standard biological parameters and to study the relation between inflammation, myocardial necrosis, lipid profile, heart rate and energy expenditure.

    Methods: Electrolytes, lipid profile, C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), procalcitonin (PCT), high-sensitive troponin T (hs-cTnT), and N-terminal pro-brain natriuretic peptide (NT-proBNP), were measured on non-fasting venous blood samples collected 8h before and after the rowing race on five healthy competitors. Heart rate and energy expenditure were measured using sporting self-measurement devices. Read More

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