6,436 results match your criteria Clinical Biochemistry [Journal]


The prognostic value of nitrotyrosine levels in coronary heart disease: long-term evaluation in the Acute Coronary Syndrome Registry Strategy (ERICO study).

Clin Biochem 2019 Feb 15. Epub 2019 Feb 15.

Center of Clinical and Epidemiological Research, Hospital Universitário, Universidade de Sao Paulo, Sao Paulo, Brazil. Electronic address:

Introduction: We aimed to analyze the association of nitrotyrosine (N-TYR) levels and long-term survival in an ongoing coronary heart disease (CHD) prospective cohort, the Acute Coronary Syndrome Registry Strategy (ERICO study).

Methods: N-TYR levels collected during acute and subacute phase from onset of acute coronary syndrome (ACS) symptoms (myocardial infarction and unstable angina) were evaluated in 342 patients. We calculated case-fatality rates (180-days, 1 year, 2 years and 4 years) and survival analyses up to 4 years using Kaplan-Meier curves and Cox regression with respective cumulative hazard ratios (95% confidence interval; 95%CI), according to N-TYR tertiles up to 4 years of follow-up. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.006DOI Listing
February 2019

SLC22A3 is associated with lipoprotein (a) concentration and cardiovascular disease in familial hypercholesterolemia.

Clin Biochem 2019 Feb 14. Epub 2019 Feb 14.

Lipids, Nutrition and Cardiovascular Prevention Clinic, Montreal Clinical Research Institute, Québec, Canada; Department of Medicine, Division of Experimental Medicine, McGill University, Québec, Canada; Department of Medicine, Division of Medical Biochemistry, McGill University, Québec, Canada. Electronic address:

Background And Aims: Several clinical and genetic factors have been shown to modulate the cardiovascular risk in subjects affected by familial hypercholesterolemia (FH). Genome wide association studies (GWAS) in the general population have identified several single nucleotide polymorphisms (SNPs) significantly associated with the risk of cardiovascular disease (CVD). This include the rs2048327 variant in the SLC22A3 gene. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.008DOI Listing
February 2019
1 Read

Correct implementation of the Hoffmann method.

Authors:
Daniel T Holmes

Clin Biochem 2019 Feb 13. Epub 2019 Feb 13.

University of British Columbia, Department of Pathology and Laboratory Medicine, Rm. G227 - 2211 Wesbrook Mall, Vancouver, BC V6T 2B5, Canada. Electronic address:

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.007DOI Listing
February 2019

Principal component and correlation analysis of biochemical and endocrine markers in a healthy pediatric population (CALIPER).

Clin Biochem 2019 Feb 11. Epub 2019 Feb 11.

CALIPER Program, Department of Pediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada; Department of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada. Electronic address:

Background: Reference intervals (i.e. normative ranges) established from a healthy reference population are essential to accurately interpret disease biomarkers. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.004DOI Listing
February 2019

Demethylation of the hypoxia induction factor 1 binding site of GPX3 at excess blood ammonia in propionic acidemia.

Clin Biochem 2019 Feb 10. Epub 2019 Feb 10.

Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Währinger Gürtel 18-20, Vienna, Austria.

Objectives: Elevated levels of metabolites such as ammonia and propionylcarnitine in propionic acidemia (PA) lead to an increased reactive oxygen species (ROS) production which could activate and stabilize the epigenetic regulated hypoxia-inducible factor-1α (HIF-1α). In order to evaluate the DNA methylation status of the HIF-1α binding site in PA, we investigated the antioxidant gluthatione peroxidase 3 gene (GPX3) promoter region.

Design And Methods: Using leukocyte DNA extracted from bloodspots collected 2-4 days after birth from diet free newborns, the cytosine phosphodiester bond guanine (CpG) dinucleotides of a HIF-1α binding site (CGTTTTTTACG) in the promoter region of GPX3 was retrospectively analysed. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.005DOI Listing
February 2019

A false positive case of high-sensitivity cardiac troponin in a patient with acute chest pain: Analytical study of the interference.

Clin Biochem 2019 Feb 6. Epub 2019 Feb 6.

UOC Chimica, Biochimica e Biologia Molecolare Clinica, Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma, Italy; Università Cattolica del Sacro Cuore, Roma, Italy.

We report a case of a heterophile antibodies interference in a new high-sensitivity troponin commercial immunoassay (cTNIH Siemens), observed in a patient with possible acute coronary syndrome (ACS). The analytical interference was investigated with standard laboratories procedures. The false positive result was found with different troponin methods and kits. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.002DOI Listing
February 2019
1 Read

Cardiac troponin T concentrations and patient-specific risk of myocardial infarction using the novel PALfx parameter.

Clin Biochem 2019 Feb 4. Epub 2019 Feb 4.

Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, SE-41345 Gothenburg, Sweden. Electronic address:

Background: Myocardial infarction (MI) is more likely if the heart damage biomarker cardiac troponin T (cTnT) is elevated in a blood sample from a patient with chest pain. There is no conventional method to estimate the risk of MI at a specific cTnT concentration. The purpose of this study was to evaluate the performance of a novel method that converts cTnT concentrations to patient-specific risks of MI. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.003DOI Listing
February 2019
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Insufficient harmonization of antibiotics assays - Polish experience with an external quality assessment program in the years 2011-2018.

Clin Biochem 2019 Feb 4. Epub 2019 Feb 4.

Department of Monitored Therapy and Pharmacogenetics, Medical University of Gdansk, Debinki 7, 80-210 Gdansk, Poland.

Introduction: Treatment with vancomycin and gentamycin requires strict monitoring of its serum concentration for proper dosage optimization. This study aimed to assess the quality and the harmonization of antibiotics assays in Polish laboratories.

Materials And Methods: 413 results of vancomycin and 148 results of gentamycin assays obtained from Polish laboratories in 30 international external quality assessment (EQA) surveys carried out from March 2011 to May 2018 were analyzed. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.02.001DOI Listing
February 2019

Impact of an electronic decision support rule on ESR/CRP co-ordering rates in a community health system and projected impact in the tertiary care setting and a commercially insured population.

Clin Biochem 2019 Jan 31. Epub 2019 Jan 31.

Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester 55905, MN, USA. Electronic address:

Introduction: Erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) are common laboratory assays used as markers of inflammation. ESR suffers from higher false positive and false negative rates than CRP. To that end, the American Board of Internal Medicine's (ABIM's) Choosing Wisely campaign has recommended against ESR testing for those with undiagnosed conditions in favor of CRP testing. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.009DOI Listing
January 2019
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Errors in measuring plasma free fatty acid concentrations with a popular enzymatic colorimetric kit.

Clin Biochem 2019 Jan 29. Epub 2019 Jan 29.

Division of Endocrinology, Diabetes and Metabolism, Endocrine Research Unit, Mayo Clinic, 200 1st Street SW, Rm 5-194 Joseph, Rochester, MN 55905, USA. Electronic address:

Objectives: Our goal was to test whether an enzymatic, colorimetric assay, the WAKO NEFA kit, provides information equivalent to liquid chromatography (LC) LC-based measures of free fatty acid (FFA).

Design & Methods: We reanalyzed nadir FFA samples from 109 volunteers from a previous study where we demonstrated that maximal suppression of FFA concentrations predicts metabolic abnormalities in humans; the results from the WAKO NEFA kit, which has been widely used for over three decades, could not replicate our findings. We conducted additional studies to directly compare results from this kit to our LC-mass spectrometry (LC/MS) method that was validated by our LC-UV detection method. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.008DOI Listing
January 2019

Fingerstick test quantifying humoral and cellular biomarkers indicative for M. leprae infection.

Clin Biochem 2019 Jan 26. Epub 2019 Jan 26.

Dept. of Infectious Diseases, Leiden University Medical Center, The Netherlands. Electronic address:

Objectives: New user-friendly diagnostic tests for detection of individuals infected by Mycobacterium leprae (M. leprae), the causative pathogen of leprosy, can help guide therapeutic and prophylactic treatment, thus positively contributing to clinical outcome and reduction of transmission. To facilitate point-of-care testing without the presence of phlebotomists, the use of fingerstick blood (FSB) rather than whole blood-derived serum is preferred. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183076
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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.007DOI Listing
January 2019
3 Reads

Aside from acute renal failure cases, are urinary markers of glomerular and tubular function useful in clinical practice?

Clin Biochem 2019 Jan 25. Epub 2019 Jan 25.

Sorbonne Université, UPMC Université Paris 06, INSERM UMRS_1155, Paris, France.; AP-HP, Service de Néphrologie et Dialyse, Hôpital Tenon, Paris, France.

The qualitative evaluation of proteinuria represents a crucial diagnostic step in clinical practice for the classification of renal diseases according to glomerular, tubulo-interstitial, mixed injury or related to monoclonal gammopathy. Combined with the quantitative evaluation, it also allows an assessment of the disease's severity and prognosis as well as the response to treatment. The development of the urine protein profile (UPP) combines specific urine protein assays on a urine spot analyzing glomerular protein markers such as albumin, transferrin and immunoglobulin G, and tubular markers such as alpha-1microglobulin and retinol binding protein, to generate a detailed quantitative and qualitative proteinuria assessment. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.006DOI Listing
January 2019
4 Reads

Proteome-wide onco-proteogenomic somatic variant identification in ER-positive breast cancer.

Clin Biochem 2019 Jan 23. Epub 2019 Jan 23.

Department of Laboratory Medicine and Pathobiology, University of Toronto, 1 King's College Circle, Toronto, ON M5S 1A8, Canada; Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Joseph and Wolf Lebovic Health Complex, 600 University Avenue, Toronto, ON M5G 1X5, Canada; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, 600 University Avenue, Toronto, ON M5G 1X5, Canada. Electronic address:

Background: Recent advances in mass spectrometric instrumentation and bioinformatics have critically contributed to the field of proteogenomics. Nonetheless, whether that integrative approach has reached the point of maturity to effectively reveal the flow of genetic variants from DNA to proteins still remains elusive. The objective of this study was to detect somatically acquired protein variants in breast cancer specimens for which full genome and transcriptome data was already available (BASIS cohort). Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.005DOI Listing
January 2019
1 Read

Unexpectedly higher diazoxon hydrolysis by serum paraoxonase-1 in coronary heart disease.

Clin Biochem 2019 Jan 16. Epub 2019 Jan 16.

Avda. Princip D'Espanya, 43892 Miami Playa, Tarragona, Spain. Electronic address:

Objectives: Low serum PON1 activities (paraoxon, phenyl-acetate or lactone substrates) are associated with coronary heart disease (CHD). We investigated the rate of diazoxon hydrolysis by PON1 in a population with CHD.

Design & Methods: Case- control study of 410 subjects with CHD and 274 controls. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.003DOI Listing
January 2019
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Circulating miR-26a, miR-106b, miR-107 and miR-133b stratify hepatocellular carcinoma patients according to their response to transarterial chemoembolization.

Clin Biochem 2019 Jan 15. Epub 2019 Jan 15.

Department of Cancer Biology, National Cancer Institute, Cairo University, Cairo, Egypt. Electronic address:

Background: A number of hepatocellular carcinoma (HCC) patients have developed resistance against transcatheter arterial chemoembolization (TACE) treatment. In this study, we aimed to develop a panel of microRNAs (miRs) biomarkers to predict clinical outcomes in HCC patients after TACE treatment.

Methods: The expression level of twenty miRs was evaluated in FFPE tissues collected from 33 HCC patients. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183091
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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.002DOI Listing
January 2019
8 Reads

Impact of clinical sample handling and processing on ultra-low level measurements of plasma cytokines.

Clin Biochem 2019 Jan 8. Epub 2019 Jan 8.

Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, United States; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115, United States. Electronic address:

Objectives: In this study, we evaluated the impact of clinical sample handling and processing on IL-6, IL-10, IFNγ, and IL-2 measurements in plasma.

Design And Methods: We collected whole blood samples and analyzed various pre-analytical parameters. We assessed the following: 1) cytokine stability in whole blood that was stored over a ten-hour period at room temperature and 4 °C; 2) cytokine stability in plasma over 6 h; 3) vigorous sample handling including repeated dropping and transport through a pneumatic transport system; and 4) freeze-thaw stability of cytokines in plasma. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2019.01.001DOI Listing
January 2019
1 Read

Comparative accuracy of optical sensor-based wearable system for non-invasive measurement of blood glucose concentration.

Clin Biochem 2019 Jan 7. Epub 2019 Jan 7.

Maale Carmel Mental Health Center Affiliated to Bruce Rappaport Medical Faculty, Technion, Tirat Carmel, Israel.

Non-invasive biosensors for indirect evaluation of routinely-measured blood components by sweat analysis have broad potential clinical applications. This trial tested a wrist-borne non-invasive glucose monitor (NIGM) to measure blood glucose (BG) levels using photoplethysmographic (PPG) optical sensors. Our aim was to determine the accuracy of the device in comparison with a standard, invasive clinical method for blood glucose monitoring. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.014DOI Listing
January 2019
6 Reads

Estimated costs of 51 commonly ordered laboratory tests in Canada.

Clin Biochem 2019 Jan 5. Epub 2019 Jan 5.

Department of Pathology and Laboratory Medicine, Calgary, Alberta, Canada; Family Medicine, Calgary, Alberta, Canada; Community Health Sciences, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada. Electronic address:

Introduction: Test cost display has been shown to reduce inappropriate laboratory test ordering practices in the United States. Unfortunately, such a system is limited in the Canadian publically funded healthcare environment. Many Canadian physicians inaccurately estimate the cost of laboratory tests, which may contribute to mis-utilization. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183105
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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.013DOI Listing
January 2019
9 Reads
2.229 Impact Factor

The estimation of uncertainty of measurement of glycated hemoglobin as an analytical performance specification and in the interpretation of its results.

Clin Biochem 2019 Jan 25;63:92-96. Epub 2018 Oct 25.

Laboratorios Galindo SC, Av. Juárez 501, Oaxaca, Oaxaca, CP 68000, Mexico.

Background: Glycated hemoglobin (HbA1c) plays a key role in monitoring the glycemic state of an individual. Uncertainty of measurement (U) indicates the magnitude of the doubt about a measurement result. To properly classify an individual as under either good or poor glycemic control, it has been suggested that U of an HbA1c result should not exceed ±0. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.10.012DOI Listing
January 2019
1 Read

Novel nonsense mutation in the α1-globin gene [HBA1:C.49A>T] is responsible for non-deletion α-thalassemia.

Clin Biochem 2019 Jan 26;63:139-142. Epub 2018 Oct 26.

Servicio de Hematología y Hemoterapia, Hospital Clínico San Carlos, Madrid, Spain.

Background: In the α-thalassemia one of the less frequent mechanisms is the nonsense mutations, which generate the substitution of a triplet that encodes an amino acid for a stop codon and, therefore, protein synthesis stops prematurely. At present, 9 mutations of this type have been documented, 6 that affect the HBA2 gene and 3 that affect the HBA1 gene.

Objectives: We present a new mutation in CD16 of the HBA1 gene, where the change AAG>TAG generates a stop codon. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.10.015DOI Listing
January 2019
2 Reads

High-sensitivity C-reactive protein and cystatin C independently and jointly predict all-cause mortality among the middle-aged and elderly Chinese population.

Clin Biochem 2018 Dec 25. Epub 2018 Dec 25.

Department of Epidemiology and Biostatistics, School of Public Health, Medical College of Soochow University, 199 Renai Road, Suzhou 215123, PR China. Electronic address:

Studies investigating the relationship between high-sensitivity C-reactive protein (hs-CRP), cystatin C, and all-cause mortality yielded inconsistent results. Moreover, the joint effect of hs-CRP and cystatin C on mortality risk is largely unknown for the general population. In this study, we examined the associations between hs-CRP, cystatin C, and all-cause mortality using data from the China Health and Retirement Longitudinal Study (CHARLS). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183107
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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.012DOI Listing
December 2018
9 Reads

Development and validation of an ultra-high performance liquid chromatography - high resolution mass spectrometry method for the quantification of total and free teicoplanin in human plasma.

Clin Biochem 2018 Dec 25. Epub 2018 Dec 25.

Department of Diagnostic sciences, Ghent University, Corneel Heymanslaan 10, 9000 Ghent, Belgium; Department of Laboratory Medicine, Ghent University Hospital, Corneel Heymanslaan 10, 9000 Ghent, Belgium. Electronic address:

Objectives: The antibiotic teicoplanin, used for the treatment of infections caused by Gram-positive bacteria, is highly bound to plasma proteins (percentage protein binding, %PB, around 90%) and therapeutic plasma levels of total teicoplanin are 10-100 mg/L. Because of the low free concentrations (i.e. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.010DOI Listing
December 2018
1 Read

Benefit of point of care testing in patient with major hyperleukocytosis.

Clin Biochem 2018 Dec 25. Epub 2018 Dec 25.

CHU Lille, Service de Biochimie automatisée Protéines, F-59000 Lille, France.

We report a case of a child with major leukocytosis (800 × 10/L) leading to a false increase in plasma potassium and an unexpected spurious decrease in sodium. To suppress interferences due to hyperleukocytosis, our laboratory protocol consists of collecting blood on Clotting Activator/Serum tubes (CAS) and/or carrying samples by human courier. CAS tube analysis showed a decreased level of hyperkalemia and sodium within the reference range (consistent with point of care measurements). Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.011DOI Listing
December 2018
7 Reads

Identification of aspirin resistance using a PDW-miR92a-score: Validation in an intermittent claudication cohort.

Clin Biochem 2019 Feb 23;64:30-36. Epub 2018 Dec 23.

Biochemistry and Immunology, Lillebaelt Hospital, Denmark; Department of Regional Health Research, University of Southern Denmark, Denmark.

Objective: Aspirin is a widely used platelet inhibitor to prevent thrombotic events. However, in 25% of patients the antiplatelet effect is insufficient. The current study aimed to validate a newly developed PDW-miR92a-score as a biomarker of the individual response to aspirin enabling targeted antithrombotic therapy. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.009DOI Listing
February 2019
1 Read

Strategies for mitigating risk posed by biotin interference on clinical immunoassays.

Clin Biochem 2018 Dec 21. Epub 2018 Dec 21.

Calgary Laboratory Services, 9, 3535 Research Rd NW, Calgary, AB T2L 2K8, Canada; Department of Pathology and Laboratory Medicine, Cumming School of Medicine, University of Calgary, Health Sciences Centre, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada.

Recently, biotin use as an oral supplement has increased significantly among the general population. Biotin is a water soluble B-vitamin and is marketed to improve the cosmetic appearance of hair, skin, and nails. In addition, high-dose biotin (>5 mg/day) is prescribed to treat inborn errors of metabolism and multiple sclerosis. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.007DOI Listing
December 2018
3 Reads

An unusual case of alpha-1-antitrypsin deficiency: SZ/Z.

Clin Biochem 2019 Feb 21;64:49-52. Epub 2018 Dec 21.

Division of Respiratory Medicine, Department of Medicine, University of Toronto, Canadian Registry for Alpha-1 Antitrypsin Deficiency, Canada.

A female patient was first seen at age 65 due to a diagnosis of alpha-1-antitrypsin deficiency (AATD). She was a lifelong non-smoker, with no significant history of second hand smoke exposure. There was no prior family history of AATD or liver disease. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183096
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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.008DOI Listing
February 2019
5 Reads

Unidentified anion gap metabolic acidosis.

Clin Biochem 2018 Dec 19. Epub 2018 Dec 19.

Department of Emergency Medicine, Mayo Clinic, Rochester, MN, United States.

A 35-month-old female with nonketotic hyperglycinemia (NKH) presented to the Emergency department with severe hypoglycemia, fever, and several episodes of seizures. Due to worsening respiratory status, additional seizures and anion gap worsening metabolic acidosis the patient was transferred to the pediatric intensive care unit. The useful mnemonics for causes of high anion gap metabolic acidosis are the classic MUDPILES (representing Methanol, Uraemia, Diabetes, Paraldehyde, Iron (and Isoniazid), Lactate, Ethylene glycol, and Salicylate) and the more recently proposed GOLD MARK (Glycols [ethylene and propylene], Oxoproline, l-lactate, d-lactate, Methanol, Aspirin, Renal failure, and Ketoacidosis) as causes of the anion gap metabolic acidosis were all ruled out. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.006DOI Listing
December 2018
2 Reads

Corrigendum to "MicroRNAs as lung cancer biomarkers and key players in lung carcinogenesis" [Clinical Biochemistry 46 (2013) 918-925].

Clin Biochem 2019 Jan 14;63:162. Epub 2018 Dec 14.

IRCCS Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (I.R.S.T.) S.r.l., Meldola 47014, FC, Italy; Department of Pediatrics, Keck School of Medicine, Norris Comprehensive Cancer Center, University of Southern California, Saban Research Institute, Children's Center for Cancer and Blood Diseases, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA; Department of Molecular Microbiology and Immunology, Keck School of Medicine, Norris Comprehensive Cancer Center, University of Southern California, Saban Research Institute, Children's Center for Cancer and Blood Diseases, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.

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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.014DOI Listing
January 2019
1 Read

Steady state errors and risk of a QC strategy.

Clin Biochem 2019 Feb 12;64:37-43. Epub 2018 Dec 12.

RCPA Quality Assurance Programs, St Leonards, Sydney, NSW, Australia 2065. Electronic address:

Background: To minimise the risk of patient harm from results, laboratories should establish QC strategies and monitor the performance of assays in line with the analytical and clinical risk.

Methods: Steady state errors were calculated from a distribution normalized for an Analytical Performance Specification expressed as Assay Capability (imprecision) minus Assay Stability (drift). Inverting this error rate gave QC run length containing one error. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183113
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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.005DOI Listing
February 2019
8 Reads

Corrigendum to "Data mining: Seasonal and temperature fluctuations in thyroid-stimulating hormone". [Clinical Biochemistry (2018) 59-63].

Clin Biochem 2019 Feb 12;64:67. Epub 2018 Dec 12.

Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, No. 1 Shuaifu Yuan, Dongcheng District, Beijing 100730, China.

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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.002DOI Listing
February 2019
1 Read

A primer on patient-based quality control techniques.

Clin Biochem 2019 Feb 11;64:1-5. Epub 2018 Dec 11.

Sengkang General Hospital, Singapore; National University Hospital, Singapore.

Background: Patient-based Quality Control techniques have been described for more than fifty years and have been widely used routinely in hematology for forty. However, because of practical issues they have not been widely utilised in clinical chemistry laboratories. But recently because of the availability of middleware and a greater appreciation of the benefits of these processes there has been a willingness to investigate their use as a QC tool. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.004DOI Listing
February 2019
2 Reads

The potential of reducing AST testing in hospital settings.

Clin Biochem 2019 Feb 10;64:57-59. Epub 2018 Dec 10.

Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada; McMaster University Medical Centre, Hamilton Health Sciences, Hamilton, Ontario, Canada.

Background: Aspartate aminotransferase (AST) is regularly ordered with alanine aminotransferase (ALT) to assess liver integrity. In many situations, AST testing provides little or no added clinical value, since ALT is more specific and the both enzyme activities highly correlate. The objective of this study is to determine the potential reduction in AST testing, if not performed when ALT results are within reference intervals (RI). Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.003DOI Listing
February 2019
1 Read

Evaluation of TERT promoter mutations in urinary cell-free DNA and sediment DNA for detection of bladder cancer.

Clin Biochem 2019 Feb 4;64:60-63. Epub 2018 Dec 4.

Department of Urology, University Hospital and Faculty of Medicine, Technische Universität Dresden, Dresden, Germany; National Center for Tumor Diseases (NCT), Partner site Dresden, Dresden, Germany. Electronic address:

Background: Cell-free DNA (cfDNA) is proposed to be a valuable source of biomarkers in liquid biopsies for various diseases as it is supposed to partially originate from tumor cells. However, data about the diagnostic implications of cfDNA in urine for the detection of bladder cancer (BCa) is sparse.

Methods: We evaluated the usability of urinary cfDNA for diagnostic purposes compared to urine sediment DNA (sDNA) in 53 BCa patients and 36 control subjects by analyzing two abundant point-mutations (C228T/C250T) in the TERT promoter using Next-Generation Sequencing. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183094
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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.009DOI Listing
February 2019
10 Reads

Overexpression of heat shock protein HSP90AA1 and translocase of the outer mitochondrial membrane TOM34 in HCV-induced hepatocellular carcinoma: A pilot study.

Clin Biochem 2019 Jan 3;63:10-17. Epub 2018 Dec 3.

Department of Biochemistry, Faculty of Medicine, Northern Border University, Arar, Saudi Arabia; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. Electronic address:

Objective: Identification of new molecular markers to enhance early diagnosis, prognosis and/or treatment of hepatocellular carcinoma (HCC) is a need. TOM34 (34 kDa-translocase of the outer mitochondrial membrane) protein expression deregulation has demonstrated to be involved in the growth of many cancers. Here, we aimed at evaluating serum TOM34 and some heat shock proteins (HSPA4, HSPA1B, and HSP90AA1) expressions in hepatitis C virus (HCV)-related cirrhosis and HCV-induced HCC relative to controls and correlating these expressions to the clinicopathological data. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183087
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http://dx.doi.org/10.1016/j.clinbiochem.2018.12.001DOI Listing
January 2019
18 Reads
2.229 Impact Factor

Circulating Gas6 is associated with reduced human carotid atherosclerotic plaque burden in high risk cardiac patients.

Clin Biochem 2019 Feb 1;64:6-11. Epub 2018 Dec 1.

Department of Medicine, Division of Cardiology, Cardiovascular Imaging Network at Queen's, Queen's University, Kingston, Ontario, Canada; Department of Biomedical and Molecular Science, Queen's University, Kingston, Ontario, Canada. Electronic address:

Objective: Pre-clinical studies suggest that growth arrest-specific protein 6 (Gas6), a member of the vitamin K dependent family of proteins, is implicated in atherosclerosis. A role for Gas6 in stabilizing atherosclerotic plaque has been suggested. Our aim was to determine the association between Gas6 and measures of carotid artery atherosclerosis in humans undergoing elective coronary angiography. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.018DOI Listing
February 2019
7 Reads

Reply to "Analytical performance assessment of a novel cartridge-based blood gas analyzer".

Clin Biochem 2019 Jan 30;63:156-157. Epub 2018 Nov 30.

Clinical Pathology Unit, ASST Fatebenefratelli-Sacco, Milan, Italy.

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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.015DOI Listing
January 2019
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Bile acid metabolism is altered in those with insulin resistance after gestational diabetes mellitus.

Clin Biochem 2019 Feb 30;64:12-17. Epub 2018 Nov 30.

Department of Clinical Biochemistry (Viapath), King's College Hospital, London, UK.

Background: Bile acids (BAs) are known mediators of glucose metabolism that are altered in type 2 diabetes mellitus (T2DM) and gestational diabetes mellitus (GDM). We hypothesised that post-prandial BA fractions are changed in women with Insulin resistance (IR) after recovery from GDM using homeostatic model assessment (HOMA-IR).

Methods: 45 women median age 44(31-47) with previous GDM, including 20 with HOMA-IR >2. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.016DOI Listing
February 2019
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Thyrotropin receptor antibody: A novel risk indicator for pregnancy loss.

Clin Biochem 2019 Feb 1;64:44-48. Epub 2018 Dec 1.

Department of Transfusion Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, PR China. Electronic address:

Background: Thyroid autoantibody has been associated with adverse pregnancy outcomes. However, thyroid-receptor antibody (TRAb) has not been considered as a potential risk assessment indicator for adverse pregnancy outcomes. Therefore, we assessed the role of TRAb in evaluation of the risk of adverse pregnancy outcomes. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.017DOI Listing
February 2019
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User competency is still a major factor affecting analytical performance of glucose meters in patient service.

Clin Biochem 2019 Jan 30;63:66-71. Epub 2018 Nov 30.

Discipline of Laboratory Medicine, Eastern Health Authority, St. John's, NL, Canada; Faculty of Medicine, Memorial University of Newfoundland, 300 Prince Philip Dr., St. John's, NL, Canada.

Objectives: This study investigated the analytical performance of glucose meters while in use for 1.5 years in a hospital setting. The effect of user competency, strip stability and strip lot number variation on the performance of blood glucose meters was investigated. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.008DOI Listing
January 2019
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Reference intervals for HbA and HbF and cut-off value of HbA for β-thalassemia carrier screening in a Guizhou population of reproductive age.

Clin Biochem 2018 Nov 29. Epub 2018 Nov 29.

Department of Laboratory, Guizhou Provincial People's Hospital, No.83 East Zhongshan Road, Guiyang 550002, Guizhou, PR China; Medical college, Guizhou University, South Jiaxiu Road, Guiyang 550025, Guizhou, PR China. Electronic address:

Objective: The aims of this study were to establish the reference intervals for HbA2 and HbF in a Guizhou population of reproductive age, and to determine the cut-off value of HbA for β-thalassemia carrier screening.

Methods: Hemoglobin analysis was performed on 832 individuals without hypochromic microcytic anemia to calculate the reference intervals for HbA and HbF. Three hundred and ninety one β-thalassemia carriers and non β-thalassemia individuals were analyzed for their HbA levels followed by detecting β-globin gene mutations, then cut-off value of HbA for β-thalassemia carrier screening was determined using ROC curve analysis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183065
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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.007DOI Listing
November 2018
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A simpler diagnostic formula for screening nonalcoholic fatty liver disease.

Clin Biochem 2019 Feb 29;64:18-23. Epub 2018 Nov 29.

Xi'an Medical University, Xi'an, China. Electronic address:

Objective: To increase the accuracy of non-invasive diagnosis of nonalcoholic fatty liver disease (NAFLD), clinical and laboratory NAFLD indicators were integrated into a diagnostic formula.

Methods: A total of 141 patients with clinically diagnosed NAFLD and 30 healthy controls were enrolled. We collected case history, body weight, height and mass index (BMI), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transpeptidase, blood urea nitrogen and blood uric acid (UA), serum creatinine, plasma total cholesterol, triglyceride, low density lipoprotein, glycosylated hemoglobin, fasting plasma glucose, fasting insulin, ultrasonic tests, Fibroscans, and other data. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.011DOI Listing
February 2019
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High-sensitivity cardiac troponin T increases after stress echocardiography.

Clin Biochem 2019 Jan 28;63:18-23. Epub 2018 Nov 28.

Department of Anesthesiology, 660 S. Euclid Ave., 63110 St. Louis, MO, United States. Electronic address:

Introduction: Exercise (ESE) and dobutamine stress echocardiography (DSE) have high sensitivity and specificity to detect inducible myocardial ischemia in patients with significant coronary artery disease (CAD). High-sensitivity cardiac troponin (hs-cTn) assays detect troponin concentrations in the ng/L range. The aim of this study was to determine the kinetics of hs-cTnT in patients undergoing ESE and DSE and possible association of hs-cTnT with inducible myocardial ischemia. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.013DOI Listing
January 2019
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RDW, NLR and RLR in predicting liver failure and prognosis in patients with hepatitis E virus infection.

Clin Biochem 2019 Jan 28;63:24-31. Epub 2018 Nov 28.

Department of Pathology and Hepatology, Beijing 302 Hospital, Beijing, China. Electronic address:

Background & Aims: Hepatitis E virus (HEV) infection contributes to substantial proportion of acute liver injury. This study aims to evaluate the ability of red cell distribution width (RDW), neutrophil to lymphocyte ratio (NLR) and RDW to lymphocyte ratio (RLR) in predicating the development of liver failure following HEV infection and the prognosis.

Methods: 93 healthy controls, 152 current/recent HEV infected patients without liver failure (HEV-non-LF) and 62 HEV patients who developed liver failure (HEV-LF) were enrolled in the study. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.012DOI Listing
January 2019
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Changes in lecithin: cholesterol acyltransferase, cholesteryl ester transfer protein and paraoxonase-1 activities in patients with colorectal cancer.

Clin Biochem 2019 Jan 28;63:32-38. Epub 2018 Nov 28.

Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Serbia.

Background: Previous studies revealed decreased level of high-density lipoprotein cholesterol (HDLC) as important factor for development of colorectal cancer (CRC). Quantity and structure of HDL particles depend on activities of lipid transfer proteins lecithin:cholesterol acyltransferase (LCAT) and cholesteryl ester transfer protein (CETP), but this topic is largely unexplored in CRC. The main objective of this study was to investigate activities of LCAT and CETP in patients with CRC. Read More

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January 2019
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Time from venipuncture to cell isolation: Impact on granulocyte-reactive antibody testing.

Clin Biochem 2019 Jan 15;63:72-78. Epub 2018 Nov 15.

Medical University of Vienna, Department of Blood Group Serology and Transfusion Medicine, Austria.

Background And Objectives: Classical neutrophil-reactive antibody testing depends on the quick isolation of neutrophils from freshly taken whole blood. To allow a better logistic preparation before testing, the influence of time interval between venipuncture and cell isolation has been evaluated in this study.

Materials And Methods: Neutrophils and whole leukocytes were isolated from EDTA whole blood immediately (T0) as well as 4, 8 and 24 h after blood donation (T4, T8 and T24). Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.10.017DOI Listing
January 2019
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Corticotropin hormone assay interference: A case series.

Clin Biochem 2019 Jan 10;63:143-147. Epub 2018 Nov 10.

Division of Endocrinology, Diabetes, Metabolism, and Nutrition, Mayo Clinic, Rochester, MN, United States. Electronic address:

Measuring the plasma corticotropin (ACTH) concentration is an important step in determining the underlying cause in patients with hypercortisolemia. Interfering substances in immunoassays can lead to erroneous results impacting clinical management. We describe a case series of 12 patients, the majority of whom were being investigated for possible Cushing's syndrome and in whom inconsistencies between the clinical picture and biochemical testing raised concerns of assay interference. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.006DOI Listing
January 2019
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Limitations of the Hoffmann method for establishing reference intervals using clinical laboratory data.

Clin Biochem 2019 Jan 9;63:79-84. Epub 2018 Nov 9.

Center for Clinical Epidemiology and Evidence-based Medicine, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.

Objective: To examine the effect of different limits of acceptability on the Hoffmann method for selecting "healthy" populations from laboratory test data.

Methods: Thyroid stimulating hormone (TSH) and free thyroxine (FT) were measured in Shunyi Maternal and Children's Hospital of Beijing Children's Hospital. The sample size of participants of TSH and FT for reference intervals (RIs) establishment was 10,864 and 10,799, respectively. Read More

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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.005DOI Listing
January 2019
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MMP7 sensitivity of mutant ECM proteins: An indicator of melanoma survival rates and T-cell infiltration.

Clin Biochem 2019 Jan 8;63:85-91. Epub 2018 Nov 8.

Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, United States; Immunology Program, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, United States. Electronic address:

Objective: To assess the potential impact of mutant ECM amino acids (AA) on melanoma-related matrix metalloproteinase-7 (MMP7) activity.

Design And Methods: We applied a novel scripted algorithm, based on the MEROPS database, to reveal mutant-dependent sensitivity changes across the cancer genome atlas, melanoma dataset.

Results: This approach revealed a strong bias in favor of mutant AA dependent protease sensitivity increases. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183091
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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.004DOI Listing
January 2019
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Baseline serum/cerebrospinal fluid ratio of carcinoembryonic antigen and carbohydrate antigen series biomarkers in non-neoplastic diseases: a cross-sectional study on 224 patients.

Clin Biochem 2019 Jan 4;63:135-138. Epub 2018 Nov 4.

Department of Neurology, Chinese PLA General Hospital, China.

Background: The measurement of carcinoembryonic antigen, carbohydrate antigen series biomarkers in cerebrospinal fluid (CSF), is useful for the diagnosis of brain metastasis and leptomeningeal metastases to a certain extent. Their serum/CSF ratios may be of benefit to earlier diagnosis and treatment. However, the normal reference values of the ratios were not available. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120183092
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http://dx.doi.org/10.1016/j.clinbiochem.2018.11.003DOI Listing
January 2019
11 Reads