Search Our Scientific Publications & Authors

J Glob Antimicrob Resist 2019 Apr 18. Epub 2019 Apr 18.

Cholera and Biofilm Research Laboratory, Pathogen Biology Group, Rajiv Gandhi Centre for Biotechnology, Thiruvanathapuram, Kerala 695014, India. Electronic address:

Objectives: Multidrug resistantKlebsiella pneumoniae with colistin resistance have been considered as a major concern in healthcare settings. The present study aims to evaluate the genome wide distribution of antibiotic resistance genes in K. pneumoniae CRKP I with high colistin resistance isolated from a patient. Read More

Microbes Infect 2019 Apr 17. Epub 2019 Apr 17.

Postdoctoral fellow, University of Copenhagen, BRIC Biotech Research & Innovation Centre, Lund Group, 2200 Copenhagen, Denmark. Electronic address:

Exp Parasitol 2019 Apr 17. Epub 2019 Apr 17.

Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, PR China. Electronic address:

The aim of this study was to observe the intestinal mucosal/systemic responses triggered by intranasal vaccination using recombinant Trichinella spiralis serine protease (rTsSP) and its capacity to elicit immune protection against larva challenge in a murine model. rTsSP coupled with cholera toxin B subunit (CTB) was used to vaccinate mice via intranasal route. The results revealed that intranasal vaccination with rTsSP plus CTB elicited significantly intestinal local sIgA response and a TsSP-specific systemic antibody response in vaccinated mice. Read More

Int J Biol Macromol 2019 Apr 16. Epub 2019 Apr 16.

Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur 741246, Nadia, West Bengal, India. Electronic address:

Type VI secretion systems (T6SS) plays a crucial role in Vibrio cholerae mediated pathogenicity. Tip of T6SS is homologous to gp27/gp5 complex or tail spike of T4 bacteriophage. VgrG-1 of V. Read More

Emerg Infect Dis 2019 May;25(5):856-864

In 2017, the exacerbation of an ongoing countrywide cholera outbreak in the Democratic Republic of the Congo resulted in >53,000 reported cases and 1,145 deaths. To guide control measures, we analyzed the characteristics of cholera epidemiology in DRC on the basis of surveillance and cholera treatment center data for 2008-2017. The 2017 nationwide outbreak resulted from 3 distinct mechanisms: considerable increases in the number of cases in cholera-endemic areas, so-called hot spots, around the Great Lakes in eastern DRC; recurrent outbreaks progressing downstream along the Congo River; and spread along Congo River branches to areas that had been cholera-free for more than a decade. Read More

Lancet Glob Health 2019 May;7(5):e568-e584

University of Maryland School of Medicine, Baltimore, MD, USA.

Background: Diarrheal diseases remain a leading cause of illness and death among children younger than 5 years in low-income and middle-income countries. The Global Enteric Multicenter Study (GEMS) has described the incidence, aetiology, and sequelae of medically attended moderate-to-severe diarrhoea (MSD) among children aged 0-59 months residing in censused populations in sub-Saharan Africa and south Asia, where most child deaths occur. To further characterise this disease burden and guide interventions, we extended this study to include children with episodes of less-severe diarrhoea (LSD) seeking care at health centres serving six GEMS sites. Read More

Open Forum Infect Dis 2019 Apr 11;6(4):ofz057. Epub 2019 Feb 11.

Infectious Diseases Division, Massachusetts General Hospital, Boston.

Background: Vibrio cholerae, the causative agent of cholera, is a major cause of diarrhea worldwide. Children under the age of 5 have the highest disease burden of cholera. Vibriocidal antibody responses following natural infection and oral cholera vaccination (OCV) are associated with protective immunity, but whether this holds uniformly true in young children is not known. Read More

Iran J Microbiol 2019 Feb;11(1):31-38

Enteric Diseases Division, Department of Microbiology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, India.

Background And Objectives: Non-typhoidal Salmonellosis, a zoonotic infection associated with acute gastroenteritis is caused by non-typhoidal salmonellae (NTS). The study was carried out to determine the prevalence of NTS serovars and their antimicrobial resistance along with the presence of the virulence gene ( gene) in poultry samples.

Materials And Methods: This is a prospective cross-sectional study carried out at the Enteric Diseases Division, Kasturba Medical College, Manipal, South India from January 2016- December 2017. Read More

Stem Cell Res Ther 2019 Apr 17;10(1):121. Epub 2019 Apr 17.

Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil.

Background: Retina and/or optic nerve injury may cause irreversible blindness, due to degeneration of retinal ganglion cells. We and others have previously shown that the intravitreal injection of mesenchymal stem cells (MSCs) protects injured retinal ganglion cells and stimulates their regeneration after optic nerve injury, but the long-term effects of this therapy are still unknown.

Methods: We injected rat MSC (rMSC) intravitreally in adult (3-5 months) Lister Hooded rats of either sex after optic nerve crush. Read More

PLoS Negl Trop Dis 2019 Apr 16;13(4):e0007263. Epub 2019 Apr 16.

Assistance Publique-Hôpitaux de Marseille (AP-HM), Marseille, France.

Background: In October 2010, Haiti was struck by a large-scale cholera epidemic. The Haitian government, UNICEF and other international partners launched an unprecedented nationwide alert-response strategy in July 2013. Coordinated NGOs recruited local rapid response mobile teams to conduct case-area targeted interventions (CATIs), including education sessions, household decontamination by chlorine spraying, and distribution of chlorine tablets. Read More

Zhongguo Zhong Yao Za Zhi 2019 Jan;44(2):405-408

Dongfang Hospital Affiliated to Beijing University of Chinese Medicine Beijing 100078,China.

Congchi Decoction in Zhouhou Beiji Fang is a prescription commonly used in treating light exterior wind-cold syndrome.Fistular Onion Stalk in the prescription has the effects in inducing sweat and dispelling exogenous evils and accelerating Yang Qi,and has been recorded in many medical books. In addition to be used to treat light exterior wind-cold syndrome,Fistular Onion Stalk is also used extensively and uniquely to treat restlessness after cholera,febrile disease,thoracic obstruction,Yin-Yang toxin syndrome,consumptive disease,bellyache due to spleen Qi deficiency according to Zhouhou Beiji Fang. Read More

Allergy 2019 Apr 16. Epub 2019 Apr 16.

Swiss Institute of Allergy and Asthma Research (SIAF), Davos, Switzerland.

Background: Many skin and mucosal inflammatory disorders, such as atopic dermatitis, have been associated with an impaired epithelial barrier function, which allows allergens, pollutants or microbes to enter the tissue and activate the immune response. The aim of this study was to establish a method to directly assess in vivo the epidermal barrier function by electrical impedance (EI) spectroscopy.

Methods: Mice epidermal barrier was damaged by epicutaneous application of proteases and cholera toxin and by tape stripping. Read More

Genes Environ 2019 5;41:11. Epub 2019 Apr 5.

1Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Tsushima-naka, Kita-ku, Okayama, 700-8530 Japan.

Background: Chloroethylnitrosourea (CENU) derivatives, such as nimustine (ACNU) and carmustine (BCNU), are employed in brain tumor chemotherapy due to their ability to cross the blood-brain barrier. They are thought to suppress tumor development through DNA chloroethylation, followed by the formation of interstrand cross-links (ICLs) that efficiently block replication and transcription. However, the alkylation of DNA and ICLs may trigger genotoxicity, leading to tumor formation as a side effect of the chemotherapeutic treatment. Read More

Int J Infect Dis 2019 Apr 12. Epub 2019 Apr 12.

Borneo Medical and Health Research Centre, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia. Electronic address:

Objectives: In this study we investigated an outbreak of Vibrio cholera O1 Ogawa serotype, occurred during December 2014 in Kudat district, situated in Sabah state of the Malaysian part of Borneo.

Methods: Active case detection and contact tracing were done at respective localities by house to house survey. Passive case detection was done among acute gastroenteritis patients attended at various health facilities. Read More

Sci Rep 2019 Apr 10;9(1):5865. Epub 2019 Apr 10.

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, United Kingdom.

Toxigenic Vibrio cholerae of the O139 serogroup have been responsible for several large cholera epidemics in South Asia, and continue to be of clinical and historical significance today. This serogroup was initially feared to represent a new, emerging V. cholerae clone that would lead to an eighth cholera pandemic. Read More

Int J Epidemiol 2019 Apr 9. Epub 2019 Apr 9.

Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.

Background: Several studies have shown that inactivated, whole-cell oral cholera vaccines (OCVs) confer both direct protection on vaccinees and herd protection on populations. Because our earlier cluster-randomized effectiveness trial (CRT) in urban Bangladesh failed to detect OCV herd protection, we reanalysed the trial to assess whether herd effects were masked in our original analysis.

Methods: A total of 267 270 persons were randomized to 90 approximately equal-sized clusters. Read More

Proc Biol Sci 2019 Apr;286(1900):20182025

1 Wellcome Sanger Institute , Wellcome Genome Campus, Hinxton, Cambridgeshire CB10 1SA , UK.

The sixth global cholera pandemic lasted from 1899 to 1923. However, despite widespread fear of the disease and of its negative effects on troop morale, very few soldiers in the British Expeditionary Forces contracted cholera between 1914 and 1918. Here, we have revived and sequenced the genome of NCTC 30, a 102-year-old Vibrio cholerae isolate, which we believe is the oldest publicly available live V. Read More

JAMA 2019 Apr;321(14):1343

J Environ Sci Health A Tox Hazard Subst Environ Eng 2019 Apr 9:1-11. Epub 2019 Apr 9.

a Department of Chemistry, Faculty of Science , University of Kinshasa , Kinshasa , Democratic Republic of the Congo.

An epidemiological survey conducted among users of water points and medical institutions in the N'djili Kilambu neighborhood of Kinshasa in Democratic Republic of the Congo, indicates that waterborne diseases have already affected more than 60% of the patients admitted to local clinics between 2013 and 2017. In order to raise public and political awareness about this hazardous health issue resulting from the lack of safely managed sanitation systems, this study investigates the microbial quality of drinking water from local water resources. Water samples were collected from nine wells and streams used as drinking sources, and analyzed for Fecal Indicator Bacteria (FIB), including Escherichia coli, Enterococcus, and Total Coliforms. Read More

Am J Trop Med Hyg 2019 Apr 8. Epub 2019 Apr 8.

Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, Penang, Malaysia.

The diarrheal disease "cholera" is caused by , and is primarily confined to endemic regions, mostly in Africa and Asia. It is punctuated by outbreaks and creates severe challenges to public health. The disease-causing strains are most-often members of serogroups O1 and O139. Read More

Integr Med (Encinitas) 2018 Apr;17(2):20-22

Facilitated by the 2018 Institute for Functional Medicine Annual International Conference, to be held May 31 through June 2, 2018, in Hollywood, Florida. For more information, please visit http://www.ifm.org/.

Toxins (Basel) 2019 Apr 6;11(4). Epub 2019 Apr 6.

Department of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, PA 18015, USA.

Cholera toxin (CT), the major virulence factor of , is an AB5 toxin secreted through the type II secretion system (T2SS). Upon secretion, the toxin initiates endocytosis through the interaction of the B pentamer with the GM1 ganglioside receptor on small intestinal cells. In addition to the release of CT in the free form, the bacteria secrete CT in association with outer membrane vesicles (OMVs). Read More

Can J Infect Dis Med Microbiol 2019 4;2019:4164982. Epub 2019 Mar 4.

Department of Microbiology, Islamic Azad University-Karaj Branch, Karaj 3148635731, Iran.

[This corrects the article DOI: 10.1155/2018/4032531.]. Read More

Mucosal Immunol 2019 Apr 5. Epub 2019 Apr 5.

Adjuvant Research Group, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, D02 R590, Ireland.

Cholera is a severe diarrheal disease caused by the bacterium Vibrio cholerae (V. cholerae) that results in 3-4 million cases globally with 100,000-150,000 deaths reported annually. Mostly confined to developing nations, current strategies to control the spread of cholera include the provision of safe drinking water and improved sanitation and hygiene, ideally in conjunction with oral vaccination. Read More

Immunobiology 2019 Mar 22. Epub 2019 Mar 22.

Division of Clinical Medicine, National Institute of Cholera and Enteric Diseases, P-33 Scheme-XM, C.I.T. Road, Beliaghata, Kolkata, 700010, India. Electronic address:

Enteric fever, caused by Salmonella enterica serovars, Typhi (S. Typhi) and Paratyphi (S. Paratyphi) is a major public health challenge for the developing nations. Read More

Org Biomol Chem 2019 Apr;17(16):4049-4060

NIDDK, LBC, National Institutes of Health, 8 Center Drive, Bethesda, MD 20892-0815, USA.

Glycoclusters displaying synthetic fragments of the O-specific polysaccharide (OSP) of Vibrio cholerae O1 serotype Inaba on a carbohydrate platform were prepared by Cu(i)-catalysed azide alkyne cycloaddition (CuAAC, click chemistry). The clusters were subsequently conjugated to BSA via squaric acid chemistry. Their immunoreactivity was compared with those of similar conventional conjugates, i. Read More

J Comp Neurol 2019 Apr 5. Epub 2019 Apr 5.

Department of Endocrinology and Metabolism & Laboratory of Endocrinology, Department of Clinical Chemistry, Amsterdam Neuroscience, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.

The hypothalamic neuropeptide Y (NPY) circuitry is a key regulator of feeding behavior. NPY also acts in the mesolimbic dopaminergic circuitry, where it can increase motivational aspects of feeding behavior through effects on dopamine output in the nucleus accumbens (NAc) and on neurotransmission in the ventral tegmental area (VTA). Endogenous NPY in the NAc originates from local interneurons and afferent projections from the hypothalamic arcuate nucleus (Arc). Read More

J Pharmacol Toxicol Methods 2019 Apr 1;97:59-66. Epub 2019 Apr 1.

Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark. Electronic address:

Introduction: Adipose tissues release adipokines, which regulate energy intake and expenditure. G protein-coupled receptors (GPCRs) and associated signaling pathways in adipocytes are potentially important drug targets for conditions with disturbed energy metabolism.

Methods: The aim of the current study was to compare signaling of endogenously expressed GPCRs between primary preadipocytes and differentiated adipocytes using a novel state-of-the-art unbiased method that measures dynamic mass redistribution (DMR) in real-time. Read More

Nature 2019 04;568(7750):10-11

Hist Cienc Saude Manguinhos 2019 Jan-Mar;26(1):187-207

Becaria postdoctoral, Instituto de Ciencias Humanas, Sociales y Ambientales/Centro Científico Tecnológico Conicet Mendoza. Mendoza - Provincia de Mendoza - Argentina. orcid.org/0000-0001-9665-6749.

The article takes a look into the disciplinary projects approved by the authorities in the province of Mendoza (Argentina) during the cholera epidemic that took place during the summer of 1886-1887. Although the projects were intended to ameliorate the sanitary conditions of the whole of the population, these were focused and applied more intensively on the underprivileged sectors and their areas of inhabitance. This follows the ideas and representations created by the State regarding the illness and its death during this period. Read More

Int J Med Microbiol 2019 Mar 22. Epub 2019 Mar 22.

Division of Pathophysiology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India. Electronic address:

Bacterial secreted proteases are the key factors that increase the virulence potential of different pathogens. Extraintestinal pathogenic E. coli (ExPEC) is a distinct pathotype that has unique ability to infect various body sites apart from the gastrointestinal tract causing several life-threatening diseases both in human and animals. Read More

Int J Environ Res Public Health 2019 Mar 27;16(7). Epub 2019 Mar 27.

Department of Environmental Health Sciences, School of Public Health & Health Sciences, University of Massachusetts Amherst, Amherst, MA 01003, USA 2 Department of Microbiology, Montana State University, Bozeman, MT 59717, USA.

The Little Bighorn River is the primary source of water for water treatment plants serving the local Crow Agency population, and has special significance in the spiritual and ceremonial life of the Crow tribe. Unfortunately, the watershed suffers from impaired water quality, with high counts of fecal coliform bacteria routinely measured during run-off events. A metagenomic analysis was carried out to identify potential pathogens in the river water. Read More

J Biomed Inform 2019 Mar 27:103160. Epub 2019 Mar 27.

Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran. Electronic address:

Crimean-Congo hemorrhagic fever (CCHF) is considered one of the major public health concerns with case fatality rates of up to 80%. Currently, there is no effective approved vaccine for CCHF. In this study, we used a computer-aided vaccine design approach to develop the first multi-epitope recombinant vaccine for CCHF. Read More

Med Mal Infect 2019 Mar 27. Epub 2019 Mar 27.

GCS biologie 85, laboratoire de biologie médicale, CHD Vendée, La Roche sur Yon, France.

Nutrition 2019 Jan 24;63-64:36-44. Epub 2019 Jan 24.

School of Community Science and Technology, Indian Institute of Engineering Science and Technology, Howrah, West Bengal, India. Electronic address:

Objectives: The role of probiotic yogurt in the prevention and treatment of hypercholesterolemia has attracted global attention. Mounting evidence has indicated that probiotics and prebiotics improve lipid metabolism by lowering low-density lipoprotein cholesterol concentration in plasma of hypercholesterolemic patients. The present study aimed to develop synbiotic soy yogurt that had a greater cholesterol-lowering effect in hypercholesterolemic mice compared with control soy yogurt. Read More

Genes Immun 2019 Mar 29. Epub 2019 Mar 29.

Centre de ressources en information scientifique, Institut Pasteur, Paris, France.

The Institut Pasteur was created, thanks to worldwide generosity with the aim to welcome and treat rabies patients, to provide a place for scientific research and to offer new teaching programs in microbiology. Louis Pasteur invited his main collaborators, who had accompanied him during his previous investigations at École Normale Supérieure, to join him in his new institute. They contributed to the principle discoveries of Pasteur, such as the fight against spontaneous generation, the identification of the ferments of putrefaction, the fight against the silk worm disease, the research on wine and beer, and the set-up of the first vaccines against avian cholera, anthrax, swine erysipelas, and rabies. Read More

Nat Commun 2019 03 27;10(1):1390. Epub 2019 Mar 27.

Department of Microbiology and Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.

The AB toxins cholera toxin (CT) from Vibrio cholerae and heat-labile enterotoxin (LT) from enterotoxigenic Escherichia coli are notorious for their roles in diarrheal disease, but their effect on other intestinal bacteria remains unexplored. Another foodborne pathogen, Campylobacter jejuni, can mimic the GM1 ganglioside receptor of CT and LT. Here we demonstrate that the toxin B-subunits (CTB and LTB) inhibit C. Read More

Int J Mol Sci 2019 Mar 26;20(6). Epub 2019 Mar 26.

Laboratory of Cellular and Molecular Physiology, Department of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Catania 95123, Italy.

Despite the relevant research efforts, the causes of amyotrophic lateral sclerosis (ALS) are still unknown and no effective cure is available. Many authors suggest that ALS is a multi-system disease caused by a network failure instead of a cell-autonomous pathology restricted to motoneurons. Although motoneuronal loss is the critical hallmark of ALS given their specific vulnerability, other cell populations, including muscle and glial cells, are involved in disease onset and progression, but unraveling their specific role and crosstalk requires further investigation. Read More

J Vet Pharmacol Ther 2019 May 25;42(3):355-360. Epub 2019 Mar 25.

College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China.

Pasteurella multocida is the causative agent of fowl cholera, and florfenicol (FF) has potent antibacterial activity against P. multocida and is widely used in the poultry industry. In this study, we established a P. Read More

Vaccine 2019 Mar 22. Epub 2019 Mar 22.

Department of Internal Medicine, Naval Hospital of Crete, Chania, Greece; Department of Theology, School of Theology, National and Kapodistrian University of Athens, Athens, Greece. Electronic address:

Cell Immunol 2019 Apr 19;338:32-42. Epub 2019 Mar 19.

Division of Biochemistry, Center for Health Disparity and Molecular Medicine, Loma Linda University, School of Medicine, Loma Linda, CA 92354, United States. Electronic address:

Autoantigen-specific immunotherapy promises effective treatment for devastating tissue specific autoimmune diseases like multiple sclerosis (MS) and type 1 diabetes (T1D). Because activated dendritic cells (DCs) stimulate the differentiation of autoreactive T cells involved in the initiation of autoimmunity, blocking the activation of DCs may be an effective strategy for inhibiting tissue specific autoimmunity. Following this approach, immature DCs were shown to remain inactive after treatment with chimeric fusion proteins composed of the cholera toxin B subunit adjuvant linked to autoantigens like proinsulin (CTB-INS). Read More

Philos Trans R Soc Lond B Biol Sci 2019 May;374(1772):20180089

1 Department of Plant and Microbial Biology, University of California , 111 Koshland Hall, Berkeley, CA 94720 , USA.

CRISPR-Cas systems function as adaptive immune systems by acquiring nucleotide sequences called spacers that mediate sequence-specific defence against competitors. Uniquely, the phage ICP1 encodes a Type I-F CRISPR-Cas system that is deployed to target and overcome PLE, a mobile genetic element with anti-phage activity in Vibrio cholerae. Here, we exploit the arms race between ICP1 and PLE to examine spacer acquisition and interference under laboratory conditions to reconcile findings from wild populations. Read More

Biochem Biophys Rep 2019 Jul 22;18:100619. Epub 2019 Feb 22.

Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.

Mouse melanoma B16-BL6 cells are useful cells for cancer metastatic studies. To understand the metastatic principle at molecular levels, it is necessary to carry out experiments in which cancer cells and their normal counterparts are compared. However, unlike normal human melanocytes, preparation of normal mouse melanocytes is quite difficult due to the lack of marketing and insufficient information on an established protocol for primary culture of mouse melanocytes. Read More

J Biotechnol 2019 Apr 18;296:83-92. Epub 2019 Mar 18.

Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences, Zhejiang Sci-Tech University, 5 Second Avenue, Xiasha Higher Education Zone, Hangzhou, 310018, China. Electronic address:

Silkworms are an economically important insect.Silkworm pupae are also a nutrient-rich food and can be used as a pharmaceutical intermediate.The N-terminus of Aβ includes 1-15 amino acid residues with a B cell surface antigen that is necessary to produce antibody and prevent the adverse reactions observed in response to the full Aβ42 peptide. Read More

Curr Infect Dis Rep 2019 Mar 20;21(4):15. Epub 2019 Mar 20.

Infectious Disease & Geographic Medicine, Redwood City Kaiser, The Kaiser Permanente Medical Group, Travel Medicine Services, 1192 Veterans Blvd, Redwood City, CA, 94063, USA.

Purpose Of Review: Infectious diseases are a risk when traveling internationally, and it is important to know the potential disease burden of a region and take appropriate preventative actions before traveling. For individuals with HIV, there are special considerations and contradictions for various vaccines and medications as well as interactions with likely antiviral drugs. The purpose of this review is to summarize the vaccine and medication recommendations for travelers with HIV infection. Read More

Arch Biochem Biophys 2019 Apr 17;665:143-151. Epub 2019 Mar 17.

Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia, 741246, West Bengal, India. Electronic address:

CgtA is an essential bacterial GTPase protein involved in multiple cellular activities. In the presence of 50S ribosome, its GTPase activity increases significantly. Through sequential deletions of CgtA protein of Vibrio cholerae (CgtA) we found that its N terminal Obg domain is essential for ribosome binding and augmenting the ribosome mediated GTPase activity. Read More

FASEB J 2019 Mar 20:fj201802652RR. Epub 2019 Mar 20.

Veterans Affairs San Diego Healthcare System, San Diego, California, USA.

Interventions that preserve motor neurons or restore functional motor neuroplasticity may extend longevity in amyotrophic lateral sclerosis (ALS). Delivery of neurotrophins may potentially revive degenerating motor neurons, yet this approach is dependent on the proper subcellular localization of neurotrophin receptor (NTR) to plasmalemmal signaling microdomains, termed membrane/lipid rafts (MLRs). We previously showed that overexpression of synapsin-driven caveolin-1 (Cav-1) ( SynCav1) increases MLR localization of NTR [ e. Read More

Am J Trop Med Hyg 2019 Mar 18. Epub 2019 Mar 18.

Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.

A comprehensive targeted intervention (CTI) was designed and deployed in the neighborhoods of cholera cases in the Kathmandu Valley with the intent of reducing rates among the neighbors of the case. This was a feasibility study to determine whether clinical centers, laboratories, and field teams were able to mount a rapid, community-based response to a case within 2 days of hospital admission. Daily line listings were requested from 15 participating hospitals during the monsoon season, and a single case initiated the CTI. Read More

Mol Microbiol 2019 Mar 15. Epub 2019 Mar 15.

LOEWE Center for Synthetic Microbiology - SYNMIKRO, Philipps-Universität Marburg, Marburg, Germany.

Vibrio cholerae is an aquatic bacterium with the potential to infect humans and cause the cholera disease. While most bacteria have single chromosomes, the V. cholerae genome is encoded on two replicons of different size. Read More

Iran J Microbiol 2018 Dec;10(6):400-408

Department of Chemistry, University of Zabol, Zabol, Iran.

Background And Objectives: The use of plants for the synthesis of nanoparticles has received attention. The present study aimed to evaluate the antibacterial effects of silver nanoparticles synthesized by leaf extract against and .

Materials And Methods: Silver nanoparticles were obtained by reacting silver nitrate solution 2 mM and leaf extract. Read More