3,452 results match your criteria Chlamydial Genitourinary Infections


Pediatric Emergency Department Testing for Gonorrhea and Chlamydia in Children.

Pediatr Emerg Care 2022 Jun 21;38(6):247-252. Epub 2022 Feb 21.

From the Department of Pediatrics, Ann & Robert H. Lurie Children's Hospital.

Objective: This study aimed to describe trends in the utilization of nucleic acid amplification (NAAT) testing for gonorrhea and chlamydia in US pediatric emergency departments. Nucleic acid amplification has been recommended over genital culture by the American Academy of Pediatrics and Centers for Disease Control and Prevention for children evaluated for sexual abuse.

Methods: We conducted a multicenter study of children aged 12 months to 11 years tested for gonorrhea and chlamydia between 2004 and 2018 at 22 hospitals in the Pediatric Health Information System. Read More

View Article and Full-Text PDF

Emergence of genital infections due to Haemophilus pittmaniae and Haemophilus sputorum.

Infect Dis Now 2022 Jun 26;52(4):227-229. Epub 2022 Apr 26.

Laboratory of Microbiology, Virgen de las Nieves University Hospital-IBS Instituto de Investigación Biosanitaria de Granada, Granada, Spain; Department of Microbiology, School of Medicine and PhD Program in Clinical Medicine and Public Health, University of Granada-IBS, Granada, PC, 18010, Spain.

Background: We report for the first time the association of Haemophilus pittmaniae and Haemophilus sputorum with urethritis in men who have sex with men and who engage in unprotected intercourse.

Patients And Methods: A search for genital pathogens was conducted using urethral exudate cultures and PCR tests for Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma spp., and Ureaplasma spp. Read More

View Article and Full-Text PDF

Heterologous prime-boost vaccination based on Polymorphic protein D protects against intravaginal Chlamydia trachomatis infection in mice.

Sci Rep 2022 04 22;12(1):6664. Epub 2022 Apr 22.

Biochemistry and Immunity Laboratory, School of Medicine, University of Cuyo, IMBECU-CONICET, Centro Universitario, 5500, Mendoza, Argentina.

The control of the worldwide spread of sexually transmitted Chlamydia trachomatis (Ct) infection urgently demands the development of a preventive vaccine. In this work, we designed a vaccine based on a fragment of polymorphic protein D (FPmpD) that proved to be immunogenic enough to generate a robust systemic and mucosal IgG humoral immune response in two strains of mice. We used a heterologous prime-boost strategy, including simultaneous systemic and mucosal administration routes. Read More

View Article and Full-Text PDF

Screening of chlamydia trachomatis in non-partner donors: situation of stored donations and proposal for periodic screening.

Reprod Biomed Online 2022 06 9;44(6):1065-1070. Epub 2021 Dec 9.

Laboratory of Clinical Biology, Ghent University Hospital, Ghent, Belgium.

Research Question: Lack of guidance on the frequency of Chlamydia trachomatis screening in non-partner donors has led to heterogeneous testing protocols. C. trachomatis was checked in sperm donations unscreened for C. Read More

View Article and Full-Text PDF

Genetic and clinical characteristics of genital Chlamydia trachomatis infection in Guangzhou, China.

Infect Genet Evol 2022 Jul 18;101:105285. Epub 2022 Apr 18.

Medical Genetic Centre, Guangdong Women and Children's Hospital, Guangzhou Medical University, Guangzhou 511442, People's Republic of China; Medical Genetic Centre, Guangdong Women and Children Hospital, Guangzhou 511442, People's Republic of China. Electronic address:

Background: Genital Chlamydia trachomatis (CT) is one of the most common agents of sexually transmitted infections and can cause severe disorders. This study aimed to analyse the genetic and clinical characteristics of genital CT infection among women in Guangzhou, China.

Methods: From September 2020 to August 2021, a total of 8955 female patients were enrolled in this study. Read More

View Article and Full-Text PDF

Towards a Deeper Understanding of Pathogenetic Mechanisms: Editorial to the Special Issue " Pathogenicity and Disease".

Int J Mol Sci 2022 Apr 1;23(7). Epub 2022 Apr 1.

Department of Public Health and Infectious Diseases, University of Rome "Sapienza", P.le Aldo Moro 5, 00185 Rome, Italy.

, an obligate intracellular Gram-negative bacterium, is characterized by a wide range of different serotypes responsible for several local or systemic human diseases, including genital tract manifestations (D-K), trachoma (A-C), and lymphogranuloma venereum (L1-3) [... Read More

View Article and Full-Text PDF

Propionibacterium acnes in urine and semen samples from men with urinary infection.

Arch Ital Urol Androl 2022 Mar 29;94(1):62-64. Epub 2022 Mar 29.

Specialist Urology and Andrology Clinic, Salerno.

Objective: Propionibacterium acnes has been implicated in the pathogenesis of prostate disease as acute and chronic prostatic inflammation, benign prostatic hyperplasia and prostate cancer although it should still be clarified if Propionibacterium acnes (P. acnes) is a commensal or accidental prostate pathogen. Aiming to evaluate the pathogenic potential for genitourinary tract of Propionibacterium acnes, we investigated the frequency of P. Read More

View Article and Full-Text PDF

Prevalence, Anatomic Distribution, and Correlates of Chlamydia trachomatis and Neisseria gonorrhoeae Infections Among a Cohort of Men Who Have Sex With Men in Hanoi, Vietnam.

Sex Transm Dis 2022 Jul 12;49(7):504-510. Epub 2022 Mar 12.

Center for Training and Research on Substance Abuse and HIV, Hanoi Medical University, Hanoi, Vietnam.

Background: Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) disproportionately affect men who have sex with men (MSM). Data on the prevalence, anatomical distribution, and correlates of NG and CT infections among MSM in Vietnam are limited.

Methods: Between July 2017 and April 2019, MSM 16 years or older without HIV were enrolled into an observational cohort study. Read More

View Article and Full-Text PDF

Interleukin-27 (IL-27) Promotes Chlamydial Infection in the Female Genital Tract.

Infect Immun 2022 04 8;90(4):e0065121. Epub 2022 Mar 8.

Department of Microbiology, Immunology, and Molecular Genetics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA.

Intravaginal infection of mice with Chlamydia muridarum has been used for investigating the mechanisms of Chlamydia trachomatis-induced pathogenicity and immune responses. In the current study, the mouse model was used to evaluate the impact of interleukin-27 (IL-27) and its receptor signaling on the susceptibility of the female genital tract to chlamydial infection. Mice deficient in IL-27 developed significantly shortened courses of chlamydial infection in the female genital tract. Read More

View Article and Full-Text PDF

Differential expression of matrix metalloproteinases in the fallopian tube of women with Chlamydia trachomatis-associated tubal ectopic pregnancy.

Microb Pathog 2022 Apr 4;165:105468. Epub 2022 Mar 4.

Molecular Microbiology Laboratory, ICMR-National Institute of Pathology, Sriramachari Bhawan, Safdarjung Hospital Campus, Post Box No. 4909, New Delhi, 110029, India. Electronic address:

Chlamydia trachomatis is an established risk factor for ectopic pregnancy (EP) in fallopian tube (FT). Matrix metalloproteinases (MMPs) have potential role in disease pathogenesis, however, dysregulation of extracellular matrix by MMPs/TIMPs (tissue inhibitors of MMPs) in infection-associated EP remains unknown. The aim was to study the expression of MMP-2, -9, -14/TIMP-1, -2, -3 in C. Read More

View Article and Full-Text PDF

Comparative Analysis of the Incidence of Selected Sexually Transmitted Bacterial Infections in Poland in 2010-2015: A Retrospective Cohort Study.

J Clin Med 2022 Feb 14;11(4). Epub 2022 Feb 14.

Chair of Medical Sciences, Faculty of Health Sciences, Lomza State University of Applied Sciences, 18-400 Lomza, Poland.

Sexually transmitted infections are common infectious diseases. The main aim of this study was to perform a comparative analysis of the incidence of bacterial sexually transmitted infections in 2010-2015 in Poland, taking into account the administrative division of the country into provinces. This was a retrospective study. Read More

View Article and Full-Text PDF
February 2022

Genital Inflammation Is Not Associated With Decreased Vaginal Tenofovir Concentrations in Women Taking Oral PrEP.

J Acquir Immune Defic Syndr 2022 04;89(4):390-395

University of California San Diego, La Jolla, CA.

Background: We evaluated the association of inflammation and dysbosis on cervicovaginal fluid (CVF) tenofovir (TFV) concentrations in women taking oral tenofovir disoproxil fumarate/emtricitable for HIV pre-exposure prophylaxis (PrEP) in the United States.

Setting: Thirty-five women in a HIV PrEP implementation study attended their week 24 visit at a San Diego research clinic and provided CVF specimens.

Methods: Women in the Adherence Enhancement Guided by Individualized Texting and Drug Levels study had their CVF specimens evaluated for (1) sexually transmitted bacterial (Neisseria gonorrhoeae, Chlamydia trachomatis, Gardnerella, and Trichomonas vaginalis), viral (human papillomavirus, cytomegalovirus and herpes simplex virus-1/2) and fungal (Candida) infections; (2) microbiome composition by 16 S sequencing (V3-V4 region); and (3) cytokine profiles by enzyme-linked immunoassay (Interleukin-8, macrophage Inflammatory protein-1a, macrophage Inflammatory Protein-1b and interferon-γ-inducible protein-10). Read More

View Article and Full-Text PDF

2021 European guideline on the management of Mycoplasma genitalium infections.

J Eur Acad Dermatol Venereol 2022 May 19;36(5):641-650. Epub 2022 Feb 19.

WHO Collaborating Centre for Gonorrhoea and other STIs, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University Hospital, Örebro University, Örebro, Sweden.

Mycoplasma genitalium infection contributes to 10-35% of non-chlamydial non-gonococcal urethritis in men. In women, M. genitalium is associated with cervicitis and pelvic inflammatory disease (PID) in 10-25%. Read More

View Article and Full-Text PDF

Combining Cellular Immunology With RNAseq to Identify Novel Chlamydia T-Cell Subset Signatures.

J Infect Dis 2022 Jun;225(11):2033-2042

Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, USA.

Chlamydia trachomatis serovars A-L cause important diseases of the eyes and reproductive tract by infecting epithelium lining those organs. A major hurdle for vaccine trials is finding a surrogate biomarker for protective immunity. Investigational data argues for T-cell biomarker(s) reflecting mucosal adaption, cytokine polarization, B-cell help, antibacterial effector mechanisms, or some combination thereof. Read More

View Article and Full-Text PDF

Galectins as potential therapeutic targets in STIs in the female genital tract.

Nat Rev Urol 2022 04 1;19(4):240-252. Epub 2022 Feb 1.

Laboratorio de Bioquímica e Inmunidad, Instituto de Bioquímica y Biotecnología, Facultad de Ciencias Médicas, Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), Universidad Nacional de Cuyo (UNCUYO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza, Argentina.

Every day, more than one million people worldwide acquire a sexually transmitted infection (STI). This public health problem has a direct effect on women's reproductive and sexual health as STIs can cause irreversible damage to fertility and can have negative consequences associated with discrimination and social exclusion. Infection with one sexually transmitted pathogen predisposes to co-infection with others, suggesting the existence of shared pathways that serve as molecular links between these diseases. Read More

View Article and Full-Text PDF

A Novel Cleavage Pattern of Complement C5 Induced by Infection the Chlamydial Protease CPAF.

Front Cell Infect Microbiol 2021 11;11:732163. Epub 2022 Jan 11.

Reproductive Medicine Center, Department of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, Changsha, China.

Urogenital infection is one of the most common bacterial sexually transmitted diseases globally. Untreated infections can ascend to the upper genital tract and establish a series of severe complications. Previous studies using C3 and C5 mice models demonstrated that C3-independent activation of C5 occurred during infection. Read More

View Article and Full-Text PDF

Evaluating the utility of the A.F. Genital System test for pathogen diagnosis in acute male urethritis.

Andrologia 2022 May 22;54(4):e14377. Epub 2022 Jan 22.

Faculty of Health Science, Avrasya University, Trabzon, Turkey.

This study evaluated the effectiveness of the A.F. Genital System (Liofilchem , Italy) in detecting pathogens compared with multiplex real-time polymerase chain reaction (PCR) in men with acute urethritis. Read More

View Article and Full-Text PDF

Single gene targeted nanopore sequencing enables simultaneous identification and antimicrobial resistance detection of sexually transmitted infections.

PLoS One 2022 21;17(1):e0262242. Epub 2022 Jan 21.

Applied Diagnostic Research and Evaluation Unit, Institute for Infection and Immunity, St George's, University of London, London, United Kingdom.

Objectives: To develop a simple DNA sequencing test for simultaneous identification and antimicrobial resistance (AMR) detection of multiple sexually transmitted infections (STIs).

Methods: Real-time PCR (qPCR) was initially performed to identify Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), Mycoplasma genitalium (MG) and Trichomonas vaginalis (TV) infections among a total of 200 vulvo-vaginal swab samples from female sex workers in Ecuador. qPCR positive samples plus qPCR negative controls for these STIs were subjected to single gene targeted PCR MinION-nanopore sequencing using the smartphone operated MinIT. Read More

View Article and Full-Text PDF
February 2022

Evidence for cGAS-STING Signaling in the Female Genital Tract Resistance to Chlamydia trachomatis Infection.

Infect Immun 2022 02 3;90(2):e0067021. Epub 2022 Jan 3.

Department of Microbiology, Immunology and Molecular Genetics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA.

Sexually transmitted Chlamydia trachomatis can ascend to the upper genital tract due to its resistance to innate immunity in the lower genital tract. C. trachomatis can activate the cGAS-STING signaling pathway in cultured cells via either cGAS or STING. Read More

View Article and Full-Text PDF
February 2022

[Urethral microbiota in healthy men and patients with urethritis].

Urologiia 2021 Dec(6):136-140

Peoples Friendship University of Russia, Department of professional Development of the Medical Men, Endoscopic Urology clinic, Moscow, Russia.

Non-gonococcal urethritis (NGU) is a common disease in men. The main cause of NGU is sexually transmitted infections, which can cause various complications, including reproductive ones. Chlamydia trachomatis and Mycoplasma genitalium are the most common causes of NGU, but no known viral or bacterial pathogens are detected in 28-50% of cases. Read More

View Article and Full-Text PDF
December 2021

Comparison of Female Genital Tract Cytokine and Microbiota Signatures Induced by Initiation of Intramuscular DMPA and NET-EN Hormonal Contraceptives - a Prospective Cohort Analysis.

Front Immunol 2021 9;12:760504. Epub 2021 Dec 9.

Institute of Infectious Disease and Molecular Medicine (IDM), University of Cape Town, Cape Town, South Africa.

Background: Cervicovaginal inflammation, bacterial microbiota and hormonal contraceptives all influence sexual and reproductive health. To date, the effects of intramuscular depo-medroxyprogesterone acetate (DMPA-IM) versus injectable norethisterone enanthate (NET-EN) on vaginal microbiota or cytokines have not been compared back-to-back, although data suggest that DMPA-IM and NET-EN have different pharmacokinetic and biologic activities. This study aimed at comparing the effects of DMPA-IM versus NET-EN initiation on cervicovaginal cytokines and microbiota in women at high risk for sexually transmitted infections (STIs) assigned to the respective contraceptives. Read More

View Article and Full-Text PDF
February 2022

Provokes Seminal Inflammation among Infertile Males.

Int J Mol Sci 2021 Dec 15;22(24). Epub 2021 Dec 15.

Andrology Centre, Tartu University Hospital, Ludvig Puusepa 8 Street, 50406 Tartu, Estonia.

The impact of sexually transmitted infections (STI) on male fertility is controversial. Aims: To investigate the prevalence of urethritis-associated STIs (chlamydia, gonorrhoeae, , trichomoniasis) among infertile males; to analyze the effect of STIs on semen parameters and blood PSA. Case-control study. Read More

View Article and Full-Text PDF
December 2021

Th1/Th17 T cell Tissue-Resident Immunity Increases Protection, But Is Not Required in a Vaccine Strategy Against Genital Infection With .

Front Immunol 2021 2;12:790463. Epub 2021 Dec 2.

Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark.

The requirement for vaccine-induced tissue-resident immunity for protection against one or repeated infections with is still not fully resolved. In this study, our aim was to investigate to which degree tissue-resident Th1/Th17 T cells in the genital tract (GT) could add to the protection mediated by circulating immunity. Out of several mucosal vaccine strategies, a strategy termed SIM (for simultaneous intrauterine and parenteral immunization with CAF01 adjuvanted CTH522), was superior in generating genital tract tissue-resident Th1/Th17 T cell immunity. Read More

View Article and Full-Text PDF
February 2022

Copan Walk Away Specimen Processor (WASP) Automated System for Pathogen Detection in Female Reproductive Tract Specimens.

Front Cell Infect Microbiol 2021 17;11:770367. Epub 2021 Nov 17.

Department of Clinical Laboratory, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.

Objective: Automation is increasingly being applied in clinical laboratories; however, preanalytical processing for microbiology tests and screening is still largely performed using manual methods owing to the complex procedures involved. To promote automation of clinical microbiology laboratories, it is important to assess the performance of automated systems for different specimen types separately. Therefore, the aim of this study was to explore the potential clinical application of the Copan Walk Away Specimen Processor (WASP) automated preanalytical microbiology processing system in the detection of pathogens in female reproductive tract specimens and its feasibility in optimizing diagnostic procedures. Read More

View Article and Full-Text PDF
January 2022

Prediction model for gonorrhea, chlamydia, and trichomoniasis in the emergency department.

Am J Emerg Med 2022 Jan 7;51:313-319. Epub 2021 Nov 7.

Department of Nutrition, Case Western Reserve University, Cleveland, OH, United States of America.

Objective: History and physical examination findings can be unreliable for prediction of genitourinary tract infections and differentiation of urinary tract infections from sexually transmitted infections (STIs). The study objective was to develop a prediction tool to more accurately identify patients with STIs.

Methods: A retrospective review of 64,490 emergency department (ED) encounters between April 18, 2014, and March 7, 2017, where patients age 18 years or older had urinalysis and urine culture or testing for gonorrhea, chlamydia, or trichomonas, was used to develop a prediction model for men and women with Neisseria gonorrhoeae or Chlamydia trachomatis, or both, and for women with Trichomonas vaginalis. Read More

View Article and Full-Text PDF
January 2022

TESTIS ABNORMALITIES IN A POPULATION OF SOUTH AUSTRALIAN KOALAS (PHASCOLARCTOS CINEREUS).

J Wildl Dis 2022 01;58(1):158-167

School of Animal and Veterinary Sciences, Faculty of Sciences, Roseworthy Campus, University of Adelaide, Roseworthy, South Australia 5371, Australia.

Testis asymmetry, in which the testes in an individual differ in size, has recently been reported in koalas (Phascolarctos cinereus) in the Mount Lofty Ranges population of South Australia. We describe the morphology and histology of both testes from affected individuals in this population (n=56) and the parameters of koalas with normal-sized testes based on age and breeding season (n=56). Morphologic measurements included testis weight, length, width, and volume; histologic parameters included seminiferous tubule diameter, seminiferous epithelial height, and seminiferous tubule (interstitial tissue ratio and presence or absence of spermatozoa). Read More

View Article and Full-Text PDF
January 2022

Triple site sexually transmitted infection testing as a crucial component of surveillance for men who have sex with men: A prospective cohort study.

Int J STD AIDS 2022 02 22;33(2):114-122. Epub 2021 Oct 22.

Crusaid Kobler AIDS Center, 26738Tel Aviv Sourasky Medical Center, Tel Aviv, Israel.

(CT) and (NG) infections are common among men who have sex with men (MSM). Many oropharyngeal and anorectal infections remain asymptomatic. We aimed to evaluate triple-site screening following PrEP introduction. Read More

View Article and Full-Text PDF
February 2022

Human genital antibody-mediated inhibition of Chlamydia trachomatis infection and evidence for ompA genotype-specific neutralization.

PLoS One 2021 18;16(10):e0258759. Epub 2021 Oct 18.

Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America.

The endocervix, the primary site of Chlamydia trachomatis (Ct) infection in women, has a unique repertoire of locally synthesized IgG and secretory IgA (SIgA) with contributions from serum IgG. Here, we assessed the ability of genital and serum-derived IgG and IgA from women with a recent positive Ct test to neutralize Ct elementary bodies (EBs) and inhibit inclusion formation in vitro in human endocervical epithelial cells. We also determined if neutralization was influenced by the major outer membrane protein (MOMP) of the infecting strain, as indicated by ompA gene sequencing and genotyping. Read More

View Article and Full-Text PDF
November 2021

Fallopian tubal infertility: the result of Chlamydia trachomatis-induced fallopian tubal fibrosis.

Mol Cell Biochem 2022 Jan 15;477(1):205-212. Epub 2021 Oct 15.

The First People's Hospital of Chenzhou, The First School of Clinical Medicine, Southern Medical University, Chenzhou, 423000, People's Republic of China.

Chlamydia trachomatis is one of the most common pathogens of sexually transmitted diseases, and its incidence in genital tract infections is now 4.7% in south China. Infertility is the end result of C. Read More

View Article and Full-Text PDF
January 2022

High Prevalence of Dominated Vaginal Microbiome Among Kenyan Secondary School Girls: Negative Effects of Poor Quality Menstrual Hygiene Management and Sexual Activity.

Front Cell Infect Microbiol 2021 21;11:716537. Epub 2021 Sep 21.

Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.

The vaginal microbiome (VMB) impacts numerous health outcomes, but evaluation among adolescents is limited. We characterized the VMB 16S rRNA gene amplicon sequencing, and its association with Bacterial vaginosis (BV) and sexually transmitted infections (STIs; chlamydia, gonorrhea, trichomoniasis) among 436 schoolgirls in Kenya, median age 16.9 years. Read More

View Article and Full-Text PDF
October 2021