115 results match your criteria Chimerism[Journal]


Lost in translation? Microchimersim detection in experimental and clinical transplantation.

Chimerism 2015 Oct;6(4):51-53

a Department of Surgery, Division of Transplantation , University of Maryland School of Medicine , Baltimore , MD , USA.

The importance of further elucidating the properties surrounding microchimerism in various experi- mental models and clinical transplantation are limited by current techniques and the sensitivity of available platforms. Development of reliable methods and use routine use of microchimerism detection in clinical practice could guide clinical decision making regarding rejection, stable function, and tolerance. Read More

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http://dx.doi.org/10.1080/19381956.2016.1241373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293316PMC
October 2015
3 Reads

Microchimerism of male origin in a cohort of Danish girls.

Chimerism 2015 Oct 11;6(4):65-71. Epub 2016 Aug 11.

d University of Copenhagen , Department of Public Health , Copenhagen , Denmark.

Male microchimerism, the presence of a small number of male cells, in women has been attributed to prior pregnancies. However, male microchimerism has also been reported in women with only daughters, in nulliparous women and prepubertal girls suggesting that other sources of male microchimerism must exist. The aim of the present study was to examine the presence of male microchimerism in a cohort of healthy nulliparous Danish girls aged 10-15 y using DNA extracted from cells from whole blood (buffy coats) and report the association with potential sources of male cells. Read More

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http://dx.doi.org/10.1080/19381956.2016.1218583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293315PMC
October 2015
27 Reads

No irradiation required: The future of humanized immune system modeling in murine hosts.

Chimerism 2015 Apr 12;6(1-2):40-5. Epub 2016 May 12.

b Department of Surgery , University of Wisconsin - Madison , Madison , WI , USA.

Immunocompromised mice are an essential tool for human xenotransplantation studies, including human haematopoietic stem cell (HSC) biology research. Over the past 35 years, there have been many advances in the development of these mouse models, offering researchers increasingly sophisticated options for creating clinically relevant mouse-human chimeras. This addendum article will focus on our recent development of the "NSGW" mouse, which, among other beneficial traits, is genetically modified to obviate the need for myeloablative irradiation of the animals. Read More

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http://dx.doi.org/10.1080/19381956.2016.1162360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063082PMC
April 2015
5 Reads

Foreword.

Authors:
Dixon B Kaufman

Chimerism 2015 Apr 22;6(1-2). Epub 2016 Apr 22.

a Ray D. Owen Professor and Chair, Division of Transplantation, University of Wisconsin - Madison , Madison , WI , USA.

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http://dx.doi.org/10.1080/19381956.2015.1176759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5064458PMC
April 2015
5 Reads

Ray Owen and the history of naturally acquired chimerism.

Authors:
Aryn Martin

Chimerism 2015 Apr 19;6(1-2):2-7. Epub 2016 Apr 19.

a Department of Sociology , York University , Toronto , Canada.

This article interweaves a history of Ray Owen's early work with a broader account of the conceptual landscape of immunology in the mid 1950's. In particular, Owen's openness to the very possibility of chimeric phenomena is recognized. Read More

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http://dx.doi.org/10.1080/19381956.2016.1168561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063071PMC
April 2015
12 Reads

Stable mixed chimerism and tolerance to human organ transplants.

Authors:
Samuel Strober

Chimerism 2015 Apr 8;6(1-2):27-32. Epub 2016 Feb 8.

a Department of Medicine , Stanford University School of Medicine , Stanford , CA , USA.

Tolerance to combined kidney and hematopoietic cell transplant has been achieved in humans after establishment of mixed chimerism allowing for the withdrawal of immunosuppressive drugs. The seminal contributions of Ray Owen provided the scientific basis for the human protocol. Read More

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http://dx.doi.org/10.1080/19381956.2015.1115584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063066PMC
April 2015
3 Reads

Facilitating cells: Translation of hematopoietic chimerism to achieve clinical tolerance.

Chimerism 2015 Apr 8;6(1-2):33-9. Epub 2016 Jan 8.

a Institute for Cellular Therapeutics, University of Louisville , Louisville , KY , USA.

For over 50 y the association between hematopoietic chimerism and tolerance has been recognized. This originated with the brilliant observation by Dr. Ray Owen that freemartin cattle twins that shared a common placental blood supply were red blood cell chimeras, which led to the discovery that hematopoietic chimerism resulted in actively acquired tolerance. Read More

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https://www.tandfonline.com/doi/full/10.1080/19381956.2015.1
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http://dx.doi.org/10.1080/19381956.2015.1130780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063065PMC
April 2015
7 Reads

Deep-sequencing of the T-cell receptor repertoire in patients with haplo-cord and matched-donor transplants.

Chimerism 2015 Jul 8;6(3):47-9. Epub 2016 Jan 8.

b Section of Hematology/Oncology, Department of Medicine, The University of Chicago , Chicago , IL , USA.

Haplo-cord transplant has emerged as a feasible and reliable approach for haematopoietic stem cell transplant in patients who are unable to find matched-donor. This approach provides fast myeloid recovery, low incidence of graft vs host disease (GVHD) and favorable graft versus leukemia (GVL) effects. T cell recovery plays an important role in preventing infectious complications; it also mediates the GVHD and the GVL effects. Read More

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http://dx.doi.org/10.1080/19381956.2015.1128624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154355PMC
July 2015
7 Reads

Microchimerism and regulation in living related kidney transplant families.

Chimerism 2014 ;5(3-4):80-5

a Department of Surgery ; School of Medicine and Public Health; University of Wisconsin ; Madison , WI USA.

Long-term harmful effects of immunosuppressive drugs and chronic rejection are a persistent impetus to establish methods to induce immunological tolerance to allografts. PCR-based studies have found evidence that humans and other placental mammals can have prolonged extremely low levels of maternal cells as well as other non-self cells, referred to as microchimerism. The persistence of these cells suggests a mechanism for the maintenance of the regulatory T-cell (Treg) responses frequently detected in offspring to non-inherited maternal antigens. Read More

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http://dx.doi.org/10.1080/19381956.2015.1111974DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063072PMC
August 2016
10 Reads

Exosomes: The missing link between microchimerism and acquired tolerance?

Chimerism 2014 17;5(3-4):63-7. Epub 2015 Dec 17.

a Department of Surgery; Division of Transplantation ; University of Wisconsin ; Madison , WI , USA.

It has become increasingly clear that the immune system of viviparous mammals is much more in the business of acquiring tolerance to non-self antigens, than it is in rejecting cells that express them (for a recent review, highlighting the role of Treg cells, see ref. (1) ). It is also clear that both self-tolerance, and acquired tolerance to non-self is a dynamic process, with a natural ebb and flow. Read More

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https://www.tandfonline.com/doi/full/10.1080/19381956.2015.1
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http://dx.doi.org/10.1080/19381956.2015.1082026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063070PMC
August 2016
6 Reads

Complete donor chimerism is a prerequisite for the effect of Predicted Indirectly ReCognizable HLA Epitopes (PIRCHE) on acute graft-versus-host disease.

Chimerism 2014 15;5(3-4):94-8. Epub 2015 Dec 15.

a Laboratory of Translational Immunology; University Medical Center Utrecht ; Utrecht , the Netherlands.

Predicted indirectly recognizable HLA epitopes (PIRCHE) computationally predict donor T-cell recognition of mismatched-HLA derived peptides following allogeneic haematopoietic stem-cell transplantation (allo-HSCT), as is evidenced by the correlation between presence of HLA-DPB1-derived PIRCHE and the occurrence of graft-vs.-host disease (GVHD). Complete donor T-cell chimerism associates with an increased GVHD risk compared to mixed patient and donor chimerism. Read More

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http://dx.doi.org/10.1080/19381956.2015.1097025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063064PMC
August 2016
13 Reads

Mixed chimerism evolution is associated with T regulatory type 1 (Tr1) cells in a β-thalassemic patient after haploidentical haematopoietic stem cell transplantation.

Chimerism 2014 9;5(3-4):75-9. Epub 2015 Dec 9.

b San Raffaele Telethon Institute for Gene Therapy (TIGET); Division of Regenerative Medicine; Stem Cells and Gene Therapy, IRCCS San Raffaele Scientific Institute ; Milan , Italy.

In a cohort of β-Thalassemia (β-Thal) transplanted with haploidentical-HSCT we identified one transplanted patient characterized by persistent mixed chimerism (PMC) for several months after HSCT. In this unique β-Thal patient we assessed the donor engraftment overtime after transplantation, the potential loss of the non-shared HLA haplotype, and the presence of CD49b(+)LAG-3(+) T regulatory type 1 (Tr1) cells, previously demonstrated to be associated with PMC after HLA-related HSCT for β-Thal. The majority of the patient's erythrocytes were of donor origin, whereas T cells were initially mostly derived from the recipient, no HLA loss, but an increased frequency of circulating Tr1 cells were observed. Read More

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http://dx.doi.org/10.1080/19381956.2015.1103423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063086PMC
August 2016
27 Reads

Positive effect of fetal cell microchimerism on tumor presentation and outcome in papillary thyroid cancer.

Chimerism 2014 ;5(3-4):106-8

a Endocrine Unit; Fondazione IRCCS Ca' Granda ; Milan , Italy.

Studies on both circulating and tissue fetal cell microchimerism (FCM) favored its protective role in thyroid cancer, consistent with findings in other malignancies. Nevertheless, scanty data were available on the possible impact on the outcome of the disease. We demonstrated that FCM has a positive effect on thyroid cancer presentation and outcome. Read More

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http://dx.doi.org/10.1080/19381956.2015.1107254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066123PMC
August 2016
8 Reads

Transient mixed chimerism for allograft tolerance.

Chimerism 2015 Apr 30;6(1-2):21-6. Epub 2015 Oct 30.

a Department of Surgery , Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School , Boston , MA , USA.

Mixed chimerism discovered in Freemartin cattle by Ray Owen 70 years ago paved the way for research on immune tolerance. Since his discovery, significant progress has been made in the effort to induce allograft tolerance via mixed chimerism in various murine models. However, induction of persistent mixed chimerism has proved to be extremely difficult in major histocompatibility complex mismatched humans. Read More

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http://dx.doi.org/10.1080/19381956.2015.1111975DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5064472PMC
April 2015
32 Reads

Tolerance to noninherited maternal antigens, reproductive microchimerism and regulatory T cell memory: 60 years after 'Evidence for actively acquired tolerance to Rh antigens'.

Chimerism 2015 Apr 30;6(1-2):8-20. Epub 2015 Oct 30.

a Division of Infectious Diseases and Perinatal Institute, Cincinnati Children's Hospital, Cincinnati , OH , USA.

Compulsory exposure to genetically foreign maternal tissue imprints in offspring sustained tolerance to noninherited maternal antigens (NIMA). Immunological tolerance to NIMA was first described by Dr. Ray D. Read More

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http://dx.doi.org/10.1080/19381956.2015.1107253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063085PMC
April 2015
5 Reads

Mixed chimerism renders residual host dendritic cells incapable of alloimmunization of the marrow donor in the canine model of allogeneic marrow transplantation.

Chimerism 2015 Oct;6(4):54-64

a Division of Clinical Research, Fred Hutchinson Cancer Research Center , Seattle , WA , USA.

This study tested whether an alloimmune response can occur in the marrow donor when infused or injected with leukocytes from their mixed chimeric transplant recipient. Two mixed chimeras were produced after conditioning with three Gray total body irradiation, donor marrow infusion, and post-grafting immunosuppression. The marrow donors were then repeatedly infused and injected with leukocytes from their respective chimeric recipient. Read More

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http://dx.doi.org/10.1080/19381956.2016.1270483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293317PMC
October 2015
4 Reads

Leukodepleted blood components do not remove the potential for long-term transfusion-associated microchimerism in Australian major trauma patients.

Chimerism 2014 7;5(3-4):86-93. Epub 2015 Aug 7.

a Australian Red Cross Blood Service ; Sydney , NSW Australia.

Despite the introduction of leukodepleted blood components, it has been shown that donor leukocyte engraftment (microchimerism) remains a long-term consequence of red blood cell (RBC) transfusion. The incidence of microchimerism may be affected by international disparities in blood processing methods or variations in transfusion practices. This study was conducted to determine the prevalence of microchimerism in Australian trauma patients. Read More

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https://www.tandfonline.com/doi/full/10.1080/19381956.2015.1
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http://dx.doi.org/10.1080/19381956.2015.1052210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063084PMC
August 2016
20 Reads

Microchimerism in women with recurrent miscarriage.

Chimerism 2014 16;5(3-4):103-5. Epub 2015 Mar 16.

a Clinical Research Division; Fred Hutchinson Cancer Research Center ; Seattle , WA USA.

Miscarriage is the most common pregnancy complication, and recurrent miscarriage (3 or more consecutive pregnancy losses) affects 1-5% of couples. Maternal-fetal exchange and the persistence of exchanged material as microchimerism appears to be disrupted in complicated pregnancies. We recently conducted a longitudinal cohort study of microchimerism in women with recurrent miscarriage. Read More

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http://dx.doi.org/10.1080/19381956.2015.1017241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154341PMC
August 2016
17 Reads

Increased maternal T cell microchimerism in the allogeneic fetus during LPS-induced preterm labor in mice.

Chimerism 2014 16;5(3-4):68-74. Epub 2015 Mar 16.

a Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research ; San Francisco , CA USA.

Fetal surgery is a promising strategy to treat fetuses with severe congenital abnormalities but its clinical applications are often limited by preterm labor. In normal pregnancy, multiple mechanisms protect the semi-allogeneic fetus from attack by maternal T cells. Maternal microchimerism (the presence of maternal cells in the fetus) has been suggested to be one mechanism of maternal-fetal tolerance in that it exposes the fetus to non-inherited maternal antigens and leads to the generation of fetal regulatory T cells that can suppress a maternal T cell response. Read More

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http://dx.doi.org/10.1080/19381956.2014.1002703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063068PMC
August 2016
20 Reads

Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry.

Chimerism 2014 30;5(3-4):99-102. Epub 2014 Oct 30.

a Laboratory for Experimental Feto-maternal Medicine; Department of Obstetrics and Fetal Medicine ; University Medical Center Hamburg-Eppendorf ; Hamburg , Germany.

Maternal microchimerism, which occurs naturally during gestation in hemochorial placental mammals upon transplacental migration of maternal cells into the fetus, is suggested to significantly influence the fetal immune system. In our previous publication, we explored the sensitivity of quantitative polymerase chain reaction and flow cytometry to detect cellular microchimerism. With that purpose, we created mixed cells suspensions in vitro containing reciprocal frequencies of wild type cells and cells positive for enhanced green fluorescent protein or CD45. Read More

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http://dx.doi.org/10.4161/19381956.2014.959827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063069PMC
August 2016
19 Reads

Feto-maternal allo-immunity, regulatory T cells and predisposition to auto-immunity. Does it all start in utero?

Chimerism 2014 ;5(2):59-62

The University of Queensland; UQ Centre for Clinical Research; Experimental Dermatology Group; Brisbane, QLD Australia.

During gestation, maternal cells traffic to the fetus leading to the natural phenomenon of microchimerism. Although their persistence in offspring has been associated with several autoimmune disorders, the precise role of maternal cells in these disorders remains unclear. We aimed to evaluate whether alloreactive maternal T cells could directly trigger a graft-vs. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199809PMC
http://dx.doi.org/10.4161/chim.29844DOI Listing
October 2015
2 Reads

Maternal microchimerism: friend or foe in type 1 diabetes?

Chimerism 2014 ;5(2):21-3

Diabetes and Metabolism Unit; School of Clinical Sciences; University of Bristol; Bristol, UK.

Increased levels of non-inherited maternal HLA alleles have been detected in the periphery of children with type 1 diabetes and an increased frequency of maternal cells have been identified in type 1 diabetes pancreas. It is now clear that the phenotype of these cells is pancreatic, supporting the hypothesis that maternal cells in human pancreas are derived from multipotent maternal progenitors. Here we hypothesize how increased levels of maternal cells could play a role in islet autoimmunity. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199804PMC
http://dx.doi.org/10.4161/chim.29870DOI Listing
May 2015
7 Reads

Donor parity no longer a barrier for female-to-male hematopoietic stem cell transplantation.

Chimerism 2014 ;5(2):56-8

Europdonor Foundation; Leiden, the Netherlands.

Allogeneic hematopoietic stem cell transplantation (HSCT) is a widely applied treatment for disorders mainly involving the hematopoietic system. The success of this treatment depends on many different patient- and donor-specific factors. Based on higher CD34+ yields and superior clinical outcomes associated with the use of male donors, males are generally seen as the preferred HSCT donor. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199808PMC
http://dx.doi.org/10.4161/chim.29562DOI Listing
October 2015
4 Reads

Does microchimerism mediate kin conflicts?

Authors:
David Haig

Chimerism 2014 ;5(2):53-5

Department of Organismic and Evolutionary Biology; Harvard University; Cambridge, MA USA.

Fetal microchimerism (FMc) is predicted to promote the fitness of the fetus and maternal microchimerism (MMc) to promote the fitness of the mother. Offspring and mothers benefit from each other's health. Therefore, microchimeric cells should usually not be harmful to their host. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199807PMC
http://dx.doi.org/10.4161/chim.29122DOI Listing
October 2015
19 Reads

Naturally acquired microchimerism: implications for transplantation outcome and novel methodologies for detection.

Chimerism 2014 ;5(2):24-39

Department of Immunohematology and Blood Transfusion; Leiden University Medical Center; Leiden, the Netherlands.

Microchimerism represents a condition where one individual harbors genetically distinct cell populations, and the chimeric population constitutes <1% of the total number of cells. The most common natural source of microchimerism is pregnancy. The reciprocal cell exchange between a mother and her child often leads to the stable engraftment of hematopoietic and non-hematopoietic stem cells in both parties. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199805PMC
http://dx.doi.org/10.4161/chim.28908DOI Listing
October 2015
16 Reads

Microchimeric fetal cells play a role in maternal wound healing after pregnancy.

Chimerism 2014 ;5(2):40-52

Anu Research Centre; Department of Obstetrics and Gynaecology; University College Cork; Cork University Maternity Hospital; Cork, Ireland.

Fetal cells persist in mothers for decades after delivery: in a phenomenon called fetal microchimerism. While persistent fetal cells were first implicated in autoimmune disease, parallel studies in animal and human pregnancy now suggest that microchimeric fetal cells play a role in the response to tissue injury. The aim of this study was to investigate the impact of fetal microchimeric cells in the adult wound, using caesarean section (CS) as a model of wound healing in pregnancy. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199806PMC
http://dx.doi.org/10.4161/chim.28746DOI Listing
October 2015
2 Reads

Maternal microchimerism in biliary atresia: are maternal cells effector cells, targets, or just bystanders?

Authors:
Toshihiro Muraji

Chimerism 2014 Jan-Mar;5(1):1-5. Epub 2014 Mar 26.

Department of Pediatric Surgery; Child Health and Cancer Research Center; Ibaraki Children's Hospital; Ibaraki, Japan.

The etiology of biliary atresia (BA) is unknown; however, the liver histology is similar to that observed in immune-mediated hepatic disorders. Liver fibrosis in BA progresses even after bile drainage has been achieved by the Kasai operation. Maternal microchimerism has been purported to play a part in the pathogenesis of BA as well as certain autoimmune diseases. Read More

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http://dx.doi.org/10.4161/chim.28576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988115PMC
December 2014
12 Reads

Pregnancy-induced maternal regulatory T cells, bona fide memory or maintenance by antigenic reminder from fetal cell microchimerism?

Chimerism 2014 Jan-Mar;5(1):16-9. Epub 2014 Feb 19.

Division of Infectious Diseases; Cincinnati Children's Hospital Medical Center; Cincinnati, OH USA.

Long-term maintenance of immune components with defined specificity, without antigen is the hallmark feature of immunological memory. However, there are fundamental differences in how memory CD8(+) compared with CD4(+) T cells are maintained. After complete antigen elimination, CD8(+) T cells can persist as a self-renewing numerically stable cell population, and therefore satisfy the most stringent definition of "memory. Read More

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http://dx.doi.org/10.4161/chim.28241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988116PMC
December 2014
6 Reads

Analysis of maternal microchimerism in rhesus monkeys (Macaca mulatta) using real-time quantitative PCR amplification of MHC polymorphisms.

Chimerism 2014 Jan-Mar;5(1):6-15. Epub 2014 Jan 17.

Division of Experimental Medicine; Department of Medicine; University of California San Francisco; San Francisco, CA USA.

Although pregnancy-associated microchimerism is known to exist in humans, its clinical significance remains unclear. Fetal microchimerism has been documented in rhesus monkeys, but the trafficking and persistence of maternal cells in the monkey fetus and infant have not been fully explored. To investigate the frequency of maternal microchimerism in the rhesus monkey (Macaca mulatta), a real-time polymerase chain reaction (PCR) strategy was developed and validated to target polymorphic major histocompatibility complex (MHC) gene sequences. Read More

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http://dx.doi.org/10.4161/chim.27778DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988117PMC
December 2014
4 Reads

Meeting report of the First Symposium on Chimerism.

Chimerism 2013 Oct-Dec;4(4):132-5. Epub 2013 Nov 18.

Fred Hutchinson Cancer Research Center; University of Washington; Seattle, WA USA.

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http://dx.doi.org/10.4161/chim.27168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921194PMC
November 2014
19 Reads

Symptotic detection of chimerism: Y does it matter?

Authors:
Peter Geck

Chimerism 2013 Oct-Dec;4(4):144-6. Epub 2013 Nov 15.

Department of Integrative Physiology and Pathobiology; Tufts University School of Medicine; Boston, MA, USA.

Microchimerism (MC), transplacental acquisition of allogeneic cells from the mother (maternofetal MC) or from the fetus (fetomaternal MC) has been in the focus of research recently. Amplicons using Y-chromosome specific SRY and DYS14 sequences have been used as markers to trace cells from a male fetus in the mother. The sensitivity of these markers in formaldehyde fixed paraffin embedded samples, however, is less than optimal. Read More

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http://dx.doi.org/10.4161/chim.27095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921198PMC
November 2014
3 Reads

Chimerism in transfusion medicine: the grandmother effect revisited.

Chimerism 2013 Oct-Dec;4(4):119-25. Epub 2013 Nov 6.

Department of Pathology; The Johns Hopkins Hospital; Baltimore, MD USA.

Transfusion therapy is complicated by the production of alloantibodies to antigens present in the donor and lacking in the recipient through the poorly-understood but likely multi-factorial process of alloimmunization. The low prevalence of alloimmunization in transfused patients (6.1%) (1) suggests that processes central to immunologic tolerance may be operating in the vast majority of transfused patients who do not produce alloantibodies. Read More

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http://dx.doi.org/10.4161/chim.26912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921192PMC
November 2014
23 Reads

Trogocytosis as a mechanistic link between chimerism and prenatal tolerance.

Chimerism 2013 Oct-Dec;4(4):126-31. Epub 2013 Oct 11.

Department of Surgery; Cincinnati Children's Hospital Medical Center; Cincinnati, OH USA.

In utero hematopoietic cellular transplantation (IUHCT) holds great promise for the treatment of congenital diseases of cellular dysfunction such as sickle cell disease, immunodeficiency disorders and inherited metabolic disorders. However, repeated failures in clinical cases of IUHCT that do not involve an immunodeficiency disease force a closer examination of the fetal immune system. While the mechanisms regulating T cell tolerance have been previously studied, the educational mechanisms leading to NK cell tolerance in prenatal chimeras remain unknown. Read More

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http://www.tandfonline.com/doi/abs/10.4161/chim.26666
Publisher Site
http://dx.doi.org/10.4161/chim.26666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921193PMC
November 2014
7 Reads

Trogocytosis in allogeneic transplants: donor cells take on the recipients identity.

Authors:
Ian M Rogers

Chimerism 2013 Oct-Dec;4(4):142-3. Epub 2013 Oct 11.

Lunenfeld-Tanenbaum Research Institute; Mount Sinai Hospital; Toronto, ON Canada; Physiology Department; University of Toronto; Toronto, ON Canada; Obstetrics and Gynaecology Department; University of Toronto; Toronto, ON Canada.

Trogocytosis has been identified as a mechanism of cell communication between immune cells. Unlike the more common receptor-ligand signaling, trogocytosis results in the transfer of intact and functional surface proteins between cells. For example, antigen presenting cells in contact with T cells exchange proteins which results in the T-cell acquiring antigen presentation capabilities. Read More

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http://dx.doi.org/10.4161/chim.26648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921197PMC
November 2014
6 Reads

The health effects of fetal microchimerism can be modeled in companion dogs.

Chimerism 2013 Oct-Dec;4(4):139-41. Epub 2013 Sep 30.

Comparative Oncology and Epigenetics Laboratory; Department of Veterinary Medicine and Surgery; University of Missouri; Columbia, MO USA.

Fetal microchimerism (FMC) has been described to have a range of effects on health and disease. Y-chromosomal DNA has been detected in Golden Retrievers suggesting persistent FMC. In that report, nine dogs had evidence of microchimerism without prior pregnancy. Read More

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http://dx.doi.org/10.4161/chim.26509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921196PMC
November 2014
5 Reads

Detection and quantification of chimerism by droplet digital PCR.

Chimerism 2013 Jul-Sep;4(3):102-8. Epub 2013 Jun 20.

Department of Pathology and Laboratory Medicine; Ann & Robert H. Lurie Children's Hospital of Chicago; Chicago, IL USA.

Accurate quantification of chimerism and microchimerism is proving to be increasingly valuable for hematopoietic cell transplantation as well as non-transplant conditions. However, methods that are available to quantify low-level chimerism lack accuracy. Therefore, we developed and validated a method for quantifying chimerism based on digital PCR technology. Read More

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http://dx.doi.org/10.4161/chim.25400DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782543PMC
April 2014
5 Reads

Complex chimerism: pregnancy after solid organ transplantation.

Chimerism 2013 Jul-Sep;4(3):71-7. Epub 2013 Jun 25.

Department of Obstetrics & Gynecology; University of Washington; Seattle, WA USA.

Thousands of women with organ transplantation have undergone successful pregnancies, however little is known about how the profound immunologic changes associated with pregnancy might influence tolerance or rejection of the allograft. Pregnant women with a solid organ transplant are complex chimeras with multiple foreign cell populations from the donor organ, fetus, and mother of the pregnant woman. We consider the impact of complex chimerism and pregnancy-associated immunologic changes on tolerance of the allograft both during pregnancy and the postpartum period. Read More

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http://dx.doi.org/10.4161/chim.25401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782547PMC
April 2014
3 Reads

Chimerism of bone marrow mesenchymal stem/stromal cells in allogeneic hematopoietic cell transplantation: is it clinically relevant?

Chimerism 2013 Jul-Sep;4(3):78-83. Epub 2013 Jul 11.

Department of Transfusion Medicine and Cell Therapy; Kyoto University Hospital; Kyoto, Japan.

Multipotent mesenchymal stem/stromal cells (MSCs) have been extensively used as a transplantable cell source for regenerative medicine and immunomodulatory therapy. Specifically in allogeneic hematopoietic stem cell transplantation (HSCT), co-transplantation or post-transplant infusion of MSCs derived from bone marrow (BM) of non-self donors has been implicated in accelerating hematopoietic recovery, ameliorating graft-vs.-host disease, and promoting tissue regeneration. Read More

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http://dx.doi.org/10.4161/chim.25609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782548PMC
April 2014
33 Reads

A discussion of immune tolerance and the layered immune system hypothesis.

Chimerism 2013 Jul-Sep;4(3):62-70. Epub 2013 May 3.

Department of Cell and Molecular Biology; Karolinska Institutet; Stockholm, Sweden.

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http://dx.doi.org/10.4161/chim.24914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782546PMC
April 2014
5 Reads

Fetal cellular microchimerism in miscarriage and pregnancy termination.

Chimerism 2013 Oct-Dec;4(4):136-8. Epub 2013 May 3.

Department of Obstetrics and Gynecology; University of Washington; Seattle, WA USA; Division of Clinical Research; Fred Hutchinson Cancer Research Center; Seattle, WA USA.

Fetal cells transfer to the mother during pregnancy and can persist long-term as microchimerism. Acquisition of microchimerism may also occur during pregnancy loss, either miscarriage or pregnancy termination. Because nearly half of all pregnancies end in loss, we recently investigated the magnitude of fetal cell transfer during pregnancy loss and whether obstetric clinical factors impacted cell transfer. Read More

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http://dx.doi.org/10.4161/chim.24915DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921195PMC
November 2014
18 Reads

Fetal microchimeric cells in autoimmune thyroid diseases: harmful, beneficial or innocent for the thyroid gland?

Chimerism 2013 Oct-Dec;4(4):111-8. Epub 2013 May 20.

Autoimmune thyroid diseases (AITD) show a female predominance, with an increased incidence in the years following parturition. Fetal microchimerism has been suggested to play a role in the pathogenesis of AITD. However, only the presence of fetal microchimeric cells in blood and in the thyroid gland of these patients has been proven, but not an actual active role in AITD. Read More

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http://dx.doi.org/10.4161/chim.25055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921191PMC
November 2014
5 Reads

Safety of treatment with DLA-identical or unrelated mesenchymal stromal cells in DLA-identical canine bone marrow transplantation.

Chimerism 2013 Jul-Sep;4(3):95-101. Epub 2013 May 29.

Clinical Research Division; Fred Hutchinson Cancer Research Center; Seattle, WA USA.

Background: Although in vitro and in vivo experiments have suggested that mesenchymal stromal cells (MSC) may have important immunomodulatory functions in allogeneic hematopoietic cell transplantation (HCT), results from clinical studies have been inconsistent. In the current study we investigate the safety of dog leukocyte antigen (DLA) identical or third party unrelated MSC in DLA-identical HCT.

Results: There were no differences between treatment groups in depth of granulocyte or platelet nadirs, time to granulocyte or platelet engraftment, rate of acute GVHD or rejection. Read More

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http://dx.doi.org/10.4161/chim.25110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782551PMC
April 2014
10 Reads

Molecular chimerism in IgE-mediated allergy: B-and T-cell tolerance toward highly immunogenic exogenous antigens.

Chimerism 2013 Jan-Mar;4(1):29-31

Division of Transplantation, Department of Surgery, Vienna General Hospital, Medical University of Vienna, Vienna, Austria.

Specific immunotherapy is the only curative treatment currently available for IgE-mediated allergy and preventive strategies are lacking altogether. We have recently reported that molecular chimerism induces durable tolerance in experimental models of allergy, thus potentially providing a new approach for the treatment and prevention of allergic diseases. Molecular chimerism is a gene-therapy approach for tolerance induction toward defined disease-causing antigens. Read More

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http://dx.doi.org/10.4161/chim.24071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654736PMC
December 2013

Combined treatment with regulatory T cells and vascularized bone marrow transplantation creates mixed chimerism and induces donor-specific tolerance to vascularized composite allografts without cytoreductive conditioning.

Chimerism 2013 Jan-Mar;4(1):20-2

Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan.

We demonstrate herein that combination treatment with regulatory T cells (Tregs) and vascularized bone marrow transplantation (VBMT) can achieve stable mixed chimerism and long-term transplantation tolerance to vascularized composite allografts (VCA) without requiring cytoreductive recipient conditioning in rats. An appreciable number of Tregs of recipient origin was shown at the interface between recipient and transplanted VCA tissues, implicating a significant role for Tregs in protecting VCA from rejection. This cytoreduction-free protocol using co-treatment with Tregs and VBMT warrants further investigation toward potential clinical application for VCA transplantation. Read More

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http://dx.doi.org/10.4161/chim.23349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654733PMC
December 2013
5 Reads

Reconsidering the bio-detection of tolerance in renal transplantation.

Chimerism 2013 Jan-Mar;4(1):15-7

Institut National de la Santé et de la Recherche Médicale INSERM U643, Nantes, France.

Operational tolerance in kidney transplantation tolerance is rare phenomenon. It concerns recipients who keep a good function of their graft without immunosuppressors for more than one year. A critical need in the field of transplantation tolerance is the identification of biomarkers able to detect precociously tolerance phenotype in stable recipient in order to adapt treatment and progressively stop immunosuppressive therapy. Read More

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http://dx.doi.org/10.4161/chim.23347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654731PMC
December 2013
28 Reads

A feasibility study on the prediction of acute graft-vs.-host disease before hematopoietic stem cell transplantation based on fetomaternal tolerance.

Chimerism 2013 Jul-Sep;4(3):84-6. Epub 2013 Sep 12.

Department of Pediatrics and Cell Transplantation; Mie University Graduate School of Medicine; Edobashi, Japan.

The contact between the immune systems of mother and child during pregnancy affects an immune response of the child against noninherited maternal antigens (NIMA) and the mother against inherited paternal antigens (IPA). However, the immunologic effects of developmental exposure to NIMA or IPA are heterogeneous, and can be either tolerogenic or immunogenic. Although we have reported that prediction of acute graft-vs. Read More

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http://dx.doi.org/10.4161/chim.24718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782549PMC
April 2014
3 Reads

Five-year clinical effects of donor bone marrow cells infusions in kidney allograft recipients: improved graft function and higher graft survival.

Chimerism 2013 Jul-Sep;4(3):87-94. Epub 2013 May 31.

Immunology Department; School of Medicine; Hamadan University of Medical Sciences; Hamadan, Iran.

Augmentation of microchimerism in solid organ transplant recipients by donor bone marrow cells (DBMC) infusion may promote immune hyporesponsiveness and consequently improve long-term allograft survival. Between March 2005 and July 2007, outcomes for 20 living unrelated donor (LURD) primary kidney recipients with concurrent DBMC infusion (an average of 2.19 ± 1. Read More

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http://dx.doi.org/10.4161/chim.24719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782550PMC
April 2014
9 Reads

Comparison of different blood sample processing methods for sensitive detection of low level chimerism by RHD real-time PCR assay.

Chimerism 2013 Jan-Mar;4(1):9-14. Epub 2013 Jan 1.

Sanquin-LUMC J.J. van Rood Center for Transfusion Medicine, Division Research, Leiden, The Netherlands.

The rhesus D blood group, which is expressed on the red blood cells (RBC) of 85% of the Caucasian population, is one of the most immunogenic RBC antigens, inducing D antibody formation in up to 20-80% of D-negative transfusion recipients and about 10% of pregnancies at risk. Pregnancy-induced D-antibodies can persist for many years, but the mechanisms underlying this persistence are unclear. The LOTUS study, a long-term follow-up study of mothers from severely affected children with hemolytic disease of the fetus and newborn investigates, among other endpoints, whether persistent feto-maternal chimerism is associated with long-term maternal anti-D persistence. Read More

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http://dx.doi.org/10.4161/chim.24248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654739PMC
December 2013
16 Reads

Microchimerism and allogeneic transplantation: we need the proof in the pudding.

Chimerism 2013 Jul-Sep;4(3):109-10. Epub 2013 Mar 19.

Weil Cornell Medical College; New York City, NY USA.

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http://dx.doi.org/10.4161/chim.24358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782544PMC
April 2014
23 Reads

Chimerism and use of mesenchymal stem cells in umbilical cord blood transplantation.

Chimerism 2013 Jan-Mar;4(1):34-5. Epub 2013 Jan 1.

Centre for Allogeneic Stem Cell Transplantation, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.

We performed a retrospective single-center analysis of 50 umbilical cord blood transplantations (UCBTs), focusing on chimerism development. Complete donor chimerism (DC) was associated with acute graft-vs.-host disease (aGVHD) grades II-IV for the CD3 (+) cell lineage (p = 0. Read More

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http://dx.doi.org/10.4161/chim.24073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654738PMC
December 2013
5 Reads